CN103704106B - A kind of vinegar residue biological active substrate for pepper seedling raising cultivation and preparation method thereof - Google Patents
A kind of vinegar residue biological active substrate for pepper seedling raising cultivation and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of vinegar residue biological active substrate for pepper seedling raising cultivation and preparation method thereof, belong to field of plant cultivation.This vinegar residue biological active substrate formula comprises mixed substrate and NEB-F fungi, and described mixed substrate carries out mixture by the peat composed of rotten mosses, vinegar grain and vermiculite by the volume of 1 ~ 3:1 ~ 3:2 to obtain, and this formula is applicable to pepper seedling raising and cultivation.The present invention reduces the consumption of the non-renewable resources peat composed of rotten mosses in growing nursery and culture matrix, and facilitate the eco-utilization of vinegar grain, reach the object reducing environmental pollution and damage by disease and insect propagation, because drawing materials in ground, not only significantly reduce the production cost of cultivation matrix, improve yield of hot pepper and quality, and applying of organic ecological soilless culture technology can be accelerated.
Description
Technical field
The invention belongs to grow seedlings of vegetable technical field of cultivation, be specifically related to a kind of vinegar residue biological active substrate formula for pepper seedling raising and cultivation.
Background technology
Along with developing rapidly of facilities horticulture, cultivation technique without soil and point disk seedling growing technology, just in large area, apply, facilitate organic solid base Quality Research, development and application on a large scale.The point disk seedling growing of China generally adopts the light ground mass material mixtures such as the peat composed of rotten mosses, vermiculite, perlite to make seedling medium, and along with the popularization of point disk seedling growing technology, the demand of the peat composed of rotten mosses constantly increases; The peat composed of rotten mosses derives from marshland, and be non-renewable resources, exhaustive exploitation can destroy the ecological balance.Therefore, utilize wide material sources, focus that the cheap research and development being convenient to again the Peat substitution matrix of scale commodity production become this field.
Zhenjiang, Jiangsu is the township of famous " aromatic vinegar ", " rice vinegar ", therefore has every year and also can produce a large amount of waste vinegar grains, pose a big pressure to ecotope.In recent years, utilize vinegar residue fermented synthesis vegetables organic raising seedling and cultivation matrix, also achieve good effect, but because pure vinegar residue substrate exists the shortcomings such as particle is thick, ventilating slit is large, 2 ~ 3 kinds of matrix mixtures should be selected during use, regulate its physicochemical character, to adapt to the needs of plant growth.
Research shows, adds PGPR can improve vegetable crop disease resistance, promote plant strain growth and improve crop yield in matrix.In order to meet the requirement to organic substrate several functions in capsicum soilless culture and point disk seedling growing, there is the necessity of research and development bioactive material further, common way is that inoculation has bioactive plant growth-promoting rhizobacteria (plantgrowthpromotingrhizobacteria, PGPR) in finished product matrix.Arbuscular mycorrhiza (arbuscularmycorrhizae, AM) is a kind of modal endotrophic mycorrhiza, also known as vesicle-arbuscular mycorrhiza (vesiculararbuscularmycorrhizae, VA), is a kind of mycorhiza in PGPR, the most extensive in distributed in nature.The current research of the majority on PGPR is all about adding the impact of bacillus subtilis (Bacillussubtilis (Ehrenberg) Cohn.) for plant in matrix, these researchs have all been paid close attention to matrix and have been added PGPR to aspects such as the effects of the impact of vegetable crop disease resistance and growth promoting effects, raising output, but also do not understand completely about the mechanism of PGPR Promoting plant growth.And at present, little to the report improving its effect about adding VA fungi in soilless culture organic matrix.
Capsicum is one of vegetables that man of China cultivated area is larger, and capsicum is main mainly with seedling raising and transplanting on producing.Although some have been had to utilize the peat composed of rotten mosses, vermiculite as the seedling medium formula of raw material domestic at present, but due to these capsicums seedling medium for various component interpolation lack system research, proportioning between each component does not have Uniform provisions, cannot meet the demand of capsicum to nutrition.Meanwhile, the current screening about PGPR and application study report are a lot, lot of documents proves, PGPR has significant facilitation to plant growth, and effect is stable, there is stronger application potential in agricultural production, but these researchs are all conceived to application PGPR develops the aspect such as biological organic fertilizer, biologic product, and utilize PGPR improving organic substrate proterties, to improve the report of its effect actually rare.
Summary of the invention
The present invention is directed to the blank in above-mentioned field, the vinegar residue biological active substrate that a kind of new pepper seedling raising is cultivated is provided, compared with conventional pepper seedling raising medium, utilize vinegar grain to replace the peat composed of rotten mosses, decrease the consumption of the non-renewable resources peat composed of rotten mosses in growing nursery and culture matrix, and facilitate the eco-utilization of vinegar grain, reach the object reducing environmental pollution and damage by disease and insect propagation, because drawing materials in ground, significantly reducing the production cost of cultivation matrix, improving yield of hot pepper and quality.
The object of the invention is to realize in the following manner:
A kind of vinegar residue biological active substrate for pepper seedling raising and cultivation, comprise mixed substrate and NEB-F fungi, this mixed substrate is carried out mixture by the volume of 1 ~ 3:1 ~ 3:2 obtained by the peat composed of rotten mosses, vinegar grain and vermiculite, preferably carries out according to the volume of 1:3:2 that mixture obtains.
The basic physicochemical character of this mixed substrate is: unit weight 0.1 ~ 0.26gcm
-3; Total pore space 54% ~ 96%; WGR 1:1.5 ~ 1:4; Nitrogen 12.83 ~ 18.75gkg
-1; Phosphorus 2.33 ~ 5.03gkg
-1; Potassium 12.07 ~ 17.26gkg
-1; PH6.26 ~ 7.0; EC1.16 ~ 1.65mScm
-1.The basic physicochemical character of preferred mixed substrate is: unit weight 0.18gcm
-3; Total pore space 63.4%; WGR 1.53; Nitrogen 17.75gkg
-1; Phosphorus 3.46gkg
-1; Potassium 17.26gkg
-1; PH6.26; EC1.65mScm
-1.
Pepper seedling nursery vinegar residue biological active substrate used is: every cubic metre of mixed substrate adds the VA fungi NEB-F fungi bacterium liquid of the 0.03-0.09% of 60 ~ 90L, and preferably every cubic metre of mixed substrate adds the VA fungi NEB-F fungi bacterium liquid of 0.03% of 75L.Seedling nursery can be point disk seedling growing.
Pepper cultivation vinegar residue biological active substrate used is: every cube of mixed substrate adds the NEB-F fungi bacterium liquid of 2.4 ~ 3.6L0.03-0.09%.The preferably NEB-F fungi bacterium liquid of every cube of mixed substrate interpolation 3L0.03%.Described pepper cultivation can be Seeding planting.
The peat composed of rotten mosses, vinegar grain and vermiculite are first carried out mixture by the volume of 1 ~ 3:1 ~ 3:2 by the preparation method of the above-mentioned vinegar residue biological active substrate for pepper seedling raising and cultivation, the 0.03-0.09%VA fungi NEB-F fungi bacterium liquid of every cubic metre of mixed substrate pouring 60 ~ 90L, after abundant mixing, for plug pepper seedling; After field planting, every cube of mixed substrate adds the NEB-F fungi bacterium liquid of 2.4 ~ 3.6L0.03-0.09%.
Preferably the peat composed of rotten mosses, vinegar grain and vermiculite are first carried out mixture by the volume of 1:3:2 by the preparation method of the above-mentioned vinegar residue biological active substrate for pepper seedling raising and cultivation, with deionized water and the commercially available fungi stoste containing 100%NEB-F bacterium liquid by volume proportioning be 0.03%VA fungi NEB-F fungi bacterium liquid, the 0.03%VA fungi NEB-F fungi bacterium liquid of every cubic metre of mixed substrate pouring 75L, after abundant mixing, for plug pepper seedling; After field planting, the bioactive material for cultivating is that every cube of mixed substrate adds 3L0.03%NEB-F fungi bacterium liquid.
Mechanism of the present invention is: along with the popularization of point disk seedling growing technology, the traditional matrices demands such as the peat composed of rotten mosses are constantly increased, but the peat composed of rotten mosses of China focuses mostly on northeastward, transport high cost when south uses, the peat composed of rotten mosses is non-renewable resources simultaneously, excessive exploitation can destroy the ecological balance, and yield and product quality decline all to some extent in recent years.And vinegar grain is as trade waste, itself just there is cheap, convenient, nutritious advantage of drawing materials, and containing a large amount of cellulose and protein, by regulating pH value, being added into microbial bacterial agent, the cultivation matrix that physicochemical property is all suitable for plant growth can be formed after fermentation.Utilize vinegar grain to substitute the peat composed of rotten mosses, be conducive to saving peat composed of rotten mosses resource, more can reduce the pollution to environment and destruction, reduce the cost of peasant planting capsicum.
The viewpoint that the current mechanism about PGPR Promoting plant growth compares accreditation has: produce plant hormone, as growth hormone, the basic element of cell division and gibberellin; Symbiotic nitrogen fixation; The mineralization of the solubilization of inorganic phosphate and organic phosphate or other nutritive element; To the antagonism of pathogenic microorganism, produce addicted to iron element, synthetic antibiotic, enzyme or Fungicidal compounds and compete nutrition with harmful microorganism.
The present invention is proved by a large amount of creative works, matrix adds VA fungi NEB-F, capsicum rhizosphere microflora is impelled to transform from " fungi type " of low fertility to " bacteria type " of high fertility, improve rhizospheric microorganism diversity and enzymic activity, contribute to the stability and the concordance that maintain the capsicum rhizosphere ecosystem, promote that capsicum is to the conversion of available nutrient and absorption, thus facilitate pepper seedling growth, and improve output.Therefore, by adding VA fungi NEB-F in matrix, be the effective way improving organic substrate effect.
Beneficial effect of the present invention compared with the prior art:
1. the present invention is a kind of vinegar residue biological active substrate formula for pepper seedling raising and cultivation, trade waste vinegar grain is adopted to replace peat composed of rotten mosses crop base starting material, decrease the consumption of the non-renewable resources peat composed of rotten mosses in growing nursery and culture matrix, and facilitate the eco-utilization of vinegar grain, reaching and reduce environmental pollution and damage by disease and insect propagation, because drawing materials in ground, not only significantly reducing the production cost of cultivation matrix, improve yield of hot pepper and quality, and applying of organic ecological soilless culture technology can be accelerated.
2. in vinegar grain mixed substrate, with the addition of VA fungi NEB-F bacterium liquid, facilitate the growth of pepper seedling and improve yield of hot pepper, under identical management condition, its Hot Pepper Seedling cultivated all is better than existing conventional seedbed system matrix after chlorophyll content in leaf blades, seedling vigorous index and field planting in pepper fruit output.
Accompanying drawing explanation
Fig. 1 is that vinegar residue biological active substrate of the present invention affects schematic diagram to output after pepper seedling field planting.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1.
T1: by the base starting materials such as the peat composed of rotten mosses, vinegar grain, vermiculite by volume proportioning become the peat composed of rotten mosses: vinegar grain: vermiculite=1:3:2, the basic physicochemical character of mixed substrate is: unit weight 0.18gcm
-3; Total pore space 63.4%; WGR 1.53; Nitrogen 17.75gkg
-1; Phosphorus 3.46gkg
-1; Potassium 17.26gkg
-1; PH6.26; EC1.65mScm
-1.The 0.03%VA fungi NEB-F of every cubic metre of mixed substrate pouring 75L, can obtain the vinegar residue biological active substrate of pepper seedling nursery of the present invention.
After capsicum field planting, by adding 0.03%NEB-F fungi bacterium liquid 3L in every cubic metre of mixed substrate, the vinegar residue biological active substrate of pepper cultivation of the present invention can be obtained.
Experimental example 2.
T2: by the base starting materials such as the peat composed of rotten mosses, vinegar grain, vermiculite by volume proportioning become the peat composed of rotten mosses: vinegar grain: vermiculite=1:3:2, the basic physicochemical character of mixed substrate is: unit weight 0.18gcm
-3; Total pore space 63.4%; WGR 1.53; Nitrogen 17.75gkg
-1; Phosphorus 3.46gkg
-1; Potassium 17.26gkg
-1; PH6.26; EC1.65mScm
-1.The 0.06%VA fungi NEB-F of every cubic metre of mixed substrate pouring 75L, can obtain the vinegar residue biological active substrate of pepper seedling nursery.After capsicum field planting, by adding 0.06%NEB-F fungi bacterium liquid 3L in every cubic metre of mixed substrate, the vinegar residue biological active substrate of pepper cultivation can be obtained.
Experimental example 3.
T3: by the base starting materials such as the peat composed of rotten mosses, vinegar grain, vermiculite by volume proportioning become the peat composed of rotten mosses: vinegar grain: vermiculite=1:3:2, the basic physicochemical character of mixed substrate is: unit weight 0.18gcm
-3; Total pore space 63.4%; WGR 1.53; Nitrogen 17.75gkg
-1; Phosphorus 3.46gkg
-1; Potassium 17.26gkg
-1; PH6.26; EC1.65mScm
-1.The 0.09%VA fungi NEB-F of every cubic metre of mixed substrate pouring 75L, can obtain the vinegar residue biological active substrate of pepper seedling nursery.After capsicum field planting, by adding 0.09%NEB-F fungi bacterium liquid 3L in every cubic metre of mixed substrate, the vinegar residue biological active substrate of pepper cultivation can be obtained.
Below application experiment is carried out to the vinegar residue biological active substrate that above-described embodiment method prepares, experimental technique and result as follows:
For examination material
(1) for examination capsicum variety: Soviet Union green pepper No. five, seed is provided by Inst of vegetables, Jiangsu Academy of Agricultural Sciences.
(2) mixed substrate raw material: vinegar grain, the peat composed of rotten mosses and vermiculite provide by Zhenjiang training flower bud matrix development in science and technology Co., Ltd; NEB-F VA Mycorrhizal Fungi purchased from American NEB (NEB) company.
Test method: by pepper seed seed soaking, vernalization, choose pepper seed sowing that is full, germination neat and consistent, employing vinegar residue substrate (peat composed of rotten mosses: vinegar grain: vermiculite=1:3:2), and carry out nursery in the mixture vinegar residue biological active substrate of every cubic metre of mixed substrate interpolation 75LNEB-F microbial inoculum (0.03% ~ 0.09%).Control seedling is in the modernization glass greenhouse of 18 DEG C ~ 28 DEG C to be cultivated.Grow to 8 true leaves until pepper seedling and after buddingging, be colonizated in cultivation tray, the strain of every basin 3.Vinegar residue substrate (the peat composed of rotten mosses: vinegar grain: vermiculite=1:3:2), and in field planting after 5 days, the mixture vinegar residue biological active substrate of adding 3LNEB-F microbial inoculum (0.03% ~ 0.09%) in pepper seedling rhizosphere carries out nursery is adopted in cultivation tray.
Investigation method: field planting after growth of seedling to 8 true leaf is buddingged, measures growth indexes; Respectively at adopting the 1st, 3,6,9,12,15 day of VA fungi NEB-F process after capsicum field planting, measure micro organism quantity, the enzymic activity of rhizosphere matrix; Process the photosynthetic gas exchange parameter and chlorophyll fluorescence parameters that within the 15th day, measure pepper plant; When pepper fruit is ripe, gathers in time, and add up single plant yield.
Interpretation of result
Table 1. vinegar residue biological active substrate of the present invention is to the effect of pepper seedling physiological growth
A, b represent the significance level that difference reaches 0.05.Lower same.
As can be seen from Table 1 with contrast the matrix (peat composed of rotten mosses: vermiculite=2:1, clear water pouring is adopted not add microbial inoculum) compare, the impact of T1, T2, T3 matrix on overground part growths such as the SL A of pepper seedling, stem are thick is little, but the under ground portion that T1, T2, T3 seedling medium all facilitates pepper seedling grows, show as that stem is thick, root dry weight increases, reach the effect of nurturing staff, this is consistent higher than CK with the pepper seedling seedling vigorous index of experimental example.Wherein the T1 seedling medium facilitation of growing to pepper seedling is comparatively large, and seedling vigorous index adds 81.25%, reaches the significance level of difference.
Table 2 vinegar residue biological active substrate of the present invention is on the impact of Pepper Leaves chlorophyll content
Chlorophyll is photosynthetic material base, and photosynthesis is one of main determining factor of phytobiology output, and therefore, chlorophyll content weighs one of whether healthy and strong important indicator of pepper seedling.As can be seen from Table 2 compared with the control, T1, T2, T3 seedling medium all improves chlorophyll a, b content and the chlorophyll total amount of Pepper Leaves, but little on the impact of chlorophyll a/chlorophyll b.Wherein T3 seedling medium is comparatively large to the facilitation of the chlorophyll total amount of Pepper Leaves, improves 124.67% compared with the control, reaches the significance level of difference.
In addition, as can be seen from Figure 1, after the pepper seedling field planting that T1 matrix is cultivated, percentage of fertile fruit increase shows as output and raises, and adds 90.1% than contrast; After the pepper seedling field planting that T2, T3 matrix is cultivated, output, also in varying degrees higher than contrast, illustrates and add the fruit yield that VA mycorhiza can significantly improve pepper seedling in matrix.
Above-mentioned test is only for illustrating technical conceive of the present invention and feature; main purpose is to announce out a kind of vinegar residue biological active substrate formula for pepper seedling raising and cultivation; person skilled in the art can be understood content of the present invention and be implemented; can not limit the scope of the invention with this; all equivalences done according to Spirit Essence of the present invention change or modify, and all should be encompassed in protection scope of the present invention.
Claims (7)
1. the vinegar residue biological active substrate for capsicum, it comprises the vinegar residue biological active substrate for pepper seedling raising and the vinegar residue biological active substrate for pepper cultivation, it is characterized in that comprising mixed substrate and NEB-F fungi, this mixed substrate carries out mixture by the peat composed of rotten mosses, vinegar grain and vermiculite by the volume of 1:3:2 to obtain; Wherein, the vinegar residue biological active substrate that pepper seedling nursery is used is: every cubic metre of mixed substrate adds the NEB-F fungi bacterium liquid of the 0.03-0.09% of 60 ~ 90L; Pepper cultivation vinegar residue biological active substrate used is: every cubic metre of mixed substrate adds the NEB-F fungi bacterium liquid of 2.4 ~ 3.6L0.03-0.09%.
2. the vinegar residue biological active substrate for capsicum according to claim 1, is characterized in that pepper seedling nursery vinegar residue biological active substrate used is: every cubic metre of mixed substrate adds the VA fungi NEB-F fungi bacterium liquid of 0.03% of 75L.
3. the vinegar residue biological active substrate for capsicum according to claim 1, is characterized in that seedling nursery is point disk seedling growing.
4. the vinegar residue biological active substrate for capsicum according to claim 1, is characterized in that pepper cultivation vinegar residue biological active substrate used is: every cube of mixed substrate adds the NEB-F fungi bacterium liquid of 3L0.03%.
5. the vinegar residue biological active substrate for capsicum according to claim 1 or 4, is characterized in that described pepper cultivation is Seeding planting.
6. the preparation method for the vinegar residue biological active substrate of capsicum, it is characterized in that first the peat composed of rotten mosses, vinegar grain and vermiculite being carried out mixture by the volume of 1:3:2, the 0.03-0.09%VA fungi NEB-F fungi bacterium liquid of every cubic metre of mixed substrate pouring 60 ~ 90L, after abundant mixing, for plug pepper seedling; After field planting, every cube of mixed substrate adds the NEB-F fungi bacterium liquid of 2.4 ~ 3.6L0.03-0.09%.
7. the preparation method of the vinegar residue biological active substrate for capsicum according to claim 6, it is characterized in that first the peat composed of rotten mosses, vinegar grain and vermiculite being carried out mixture by the volume of 1:3:2, the 0.03%VA fungi NEB-F fungi bacterium liquid of every cubic metre of mixed substrate pouring 75L, after abundant mixing, for plug pepper seedling; After field planting, every cube of mixed substrate adds the NEB-F fungi bacterium liquid of 3L0.03%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104082025B (en) * | 2014-07-24 | 2016-03-23 | 寿光市新世纪种苗有限公司 | A kind of method of cultivating the disease-resistant seedling of color green pepper |
| CN105875303A (en) * | 2016-04-08 | 2016-08-24 | 西双版纳傣族自治州农业科学研究所 | Method for mechanically raising rice seedlings |
| CN106478297A (en) * | 2016-10-17 | 2017-03-08 | 北京绿穑生物科技有限责任公司 | A kind of novel microbial seedling medium and its preparation method and application |
| CN112960995A (en) * | 2021-03-03 | 2021-06-15 | 太仓绿丰农业资源开发有限公司 | Method for producing organic fertilizer by airflow membrane fermentation of tailed vegetables, product and application thereof |
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| CN102652487A (en) * | 2012-05-11 | 2012-09-05 | 江苏大学 | Method for resisting blight by combining mycorrhizal fungi and antagonistic bacteria in cultivating vinegar residue matrix |
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