CN110291957A - It is a kind of based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate and its application method - Google Patents

It is a kind of based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate and its application method Download PDF

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CN110291957A
CN110291957A CN201910622452.6A CN201910622452A CN110291957A CN 110291957 A CN110291957 A CN 110291957A CN 201910622452 A CN201910622452 A CN 201910622452A CN 110291957 A CN110291957 A CN 110291957A
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bacteria residue
sparassis crispa
cucumber seedling
raising substrate
seedling
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CN110291957B (en
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王涛
陈永快
雷锦桂
廖水兰
黄语燕
兰婕
吴宝意
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Scientific Technical Personnel Training Center Fujian Academy Of Agricultural Sciences
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Scientific Technical Personnel Training Center Fujian Academy Of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/23Wood, e.g. wood chips or sawdust
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

It is the cucumber seedling-raising substrate and its application method of raw material that the present invention provides a kind of based on Sparassis crispa bacteria residue, the cucumber seedling-raising substrate is 1: 1: 1~3: 1: 1 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf by forming from decomposed Sparassis crispa bacteria residue, perlite and turf.The cucumber seedling-raising substrate is that A:Ca (NO is added in every 200L water using the formula of nutrient solution for cultivating cucumber seedling, seedling raising process3)2For 16kg, KNO3For 6kg;B:KH2PO4For 3kg, MgSO4For 10kg, EDTA-Fe 460g, MnSO4For 120g, ZnSO4For 60g, CuSO4For 4g, (NH4)6Mo7O24·4H2O is 1.5g, H3BO3For 60g.So that cucumber seedling-raising substrate performance is good, the cucumber yield cultivated is high.

Description

It is a kind of based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate and its application method
Technical field
The present invention relates to batch production cucumber seedling-raising technical fields, are specifically designed a kind of Huang based on Sparassis crispa bacteria residue for raw material Melon seedling medium and its application method.
Background technique
In recent years, with the rapid development of China's industrialized agriculture, soilless cultivation mode is promptly promoted and applied, cucumber It is one of main cultivated crop.Vegetable soilless culture is then to transplant the factorial production using after plastics cave dish substrate seedling, because This, industrial hole plate substrate seedling is the basis of soilless cultivation.The matrix that hole plate seedling growth uses is wanted to create for root system good The conditions such as water, gas, fertilizer, therefore to select the matrix of light weight, retentiveness, good permeability.
Seedling medium is mainly based on turf, and turf is rich in organic matter, and quality is light, retentiveness and good permeability, is a kind of Ideal seedling medium.But turf is expensive and non-renewable, and exhaustive exploitation can make resource exhausted, causes serious ecological ring Border problem.To reduce production cost, cheap material is selected partially or completely to substitute the turf in matrix formulations, is formed Available vegetable plug seedling compounding matrix formulations become the hot spot of China's research in production.
Bacteria residue refers to remaining culture medium fertilizer after edible mushroom harvest.Currently, bacteria residue be primarily used to cultivation matrix, Feed, organic fertilizer, ecological environment repair materials, soil improvement etc., but bacteria residue in above-mentioned application using limited and grind Study carefully that depth is inadequate, is in this way both the waste to resource, serious pollution can be also caused to environment, therefore make good use of bacteria residue and be not only Resource utilization problem, and be also of great significance to environmental protection.
Bacteria residue currently used for crop seedling has mushroom residue, oyster mushroom mushroom slag, agrocybe mushroom slag, Pleurotus eryngii mushroom slag, acupuncture needle Mushroom mushroom slag, straw mushroom mushroom slag and seafood mushroom mushroom slag etc., different cultivars bacteria residue physicochemical property difference is larger, matrix formulations and crop varieties There is larger difference, and has no that Sparassis crispa bacteria residue is reported as seedling medium.Sparassis crispa is a kind of Rare edible fungus, is known as " kings of ten thousand mushrooms " are the very rare rare dual-purpose of drug and food mushroom in the world, vitamin, minerals and polyphenoils rich in Matter, medical value are high.China is the country that third successfully cultivates Sparassis crispa, and Fujian Province is as the big province of Edible Fungi, mesh Sparassis crispa batch production volume production is realized in preceding multiple areas.Sparassis crispa bacteria residue after harvesting has wide on soilless substyate culture Application prospect, develops cultivation matrix with bacteria residue, can turn waste into wealth, and abundant waste secondary use has significant ecological benefits.
Summary of the invention
The technical problems to be solved by the present invention are: providing a kind of cucumber seedling-raising substrate based on Sparassis crispa bacteria residue for raw material And its application method can reduce turf in the actual production process instead of conventional soilless raising seedling substrate to a certain extent It uses, saves and use turf resource, and the cucumber seedling planted is healthy and strong, while also can promote the resource of Sparassis crispa bacteria residue Change is recycled, and has apparent economic benefit, social benefit and ecological benefits.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention are as follows:
A kind of is the cucumber seedling-raising substrate of raw material based on Sparassis crispa bacteria residue, and the cucumber seedling-raising substrate is by from decomposed silk ball Bacterium bacteria residue, perlite and turf composition, are 1: 1: 1~3: 1: 1 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf.
The beneficial effects of the present invention are:
1, the present invention is creatively selected from decomposed Sparassis crispa bacteria residue as matrix raw material, it is decomposed after Sparassis crispa bacteria residue Cleaning, being compounded in physicochemical property with turf, perlite realizes good complementary, has played respective advantage, obtained Huang Melon seedling medium is suitable in aspect of performance such as conductivity, bulk density, total porosity, aeration porosity, water-holding porosity, pH values In cucumber seedling-raising;Volume ratio from decomposed Sparassis crispa bacteria residue and turf, perlite is compound for cucumber to educate at 1: 1: 1~3: 1: 1 The planting percent of Miao Shi, cucumber seedling are high, and the indexs such as soluble sugar, root activity and yield of cucumber seedling are best;
2, the present invention provides a kind of new approaches, obtained cucumber for the recycling recycling of discarded Sparassis crispa bacteria residue Seedling medium can partially substitute turf, reduce seedling cost;It is environmentally protective, applying pesticides are not necessarily in seedling raising process;Germ, worm's ovum It is few, be conducive to the cucumber seedling for cultivating health.
A kind of application method based on the cucumber seedling-raising substrate that Sparassis crispa bacteria residue is raw material, the cucumber seedling-raising substrate are used In cultivating cucumber seedling, seedling raising process uses nutrient solution, and the formula of the nutrient solution is that A:Ca (NO is added in every 200L water3)2For 16kg, KNO3For 6kg;B:KH2PO4For 3kg, MgSO4For 10kg, EDTA-Fe 460g, MnSO4For 120g, ZnSO4For 60g, CuSO4For 4g, (NH4)6Mo7O24·4H2O is 1.5g, H3BO3For 60g.
The beneficial effects of the present invention are: the cucumber seedling-raising substrate and above-mentioned nutritious liquid prescription use, the Huang of cultivation For melon seedling in later period cultivation production test, fruit yield is better than conventional seedbed system formula, increases economic efficiency.
Detailed description of the invention
Fig. 1 is influence of the cucumber seedling-raising substrate of eight kinds of formulas of the embodiment of the present invention to cucumber seedling emergence rate;
Fig. 2 is influence of the cucumber seedling-raising substrate of eight kinds of formulas of the embodiment of the present invention to cucumber seedling root volume;
Fig. 3 is influence of the cucumber seedling-raising substrate of eight kinds of formulas of the embodiment of the present invention to cucumber seedling leaf area.
Specific embodiment
To explain the technical content, the achieved purpose and the effect of the present invention in detail, below in conjunction with embodiment and cooperate attached Figure is explained.
A kind of is the cucumber seedling-raising substrate of raw material based on Sparassis crispa bacteria residue, and the cucumber seedling-raising substrate is by from decomposed silk ball Bacterium bacteria residue, perlite and turf composition, are 1: 1: 1~3: 1: 1 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf.
Using the Sparassis crispa bacteria residue after harvesting contain organic matter abundant and N, P, K element the characteristics of, it is decomposed after Sparassis crispa Bacteria residue is clean, and being compounded in physicochemical property with turf, perlite realizes good complementary, has played respective advantage, obtained Cucumber seedling-raising substrate the aspect of performance such as conductivity, bulk density, total porosity, aeration porosity, water-holding porosity, pH value all It is suitable for cucumber seedling-raising.
It wherein, is 1: 1~3: 1 from the volume ratio of decomposed Sparassis crispa bacteria residue and perlite.
Preferably, it is 1 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf in the cucumber seedling-raising substrate: 1∶1。
Preferably, it is 2 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf in the cucumber seedling-raising substrate: 1∶1。
Preferably, it is 3 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf in the cucumber seedling-raising substrate: 1∶1。
Preferably, in the cucumber seedling-raising substrate, from the partial size of decomposed Sparassis crispa bacteria residue are as follows: 2mm.
Specifically, the raw material from decomposed Sparassis crispa bacteria residue is the cultivation remaining waste of Sparassis crispa, by quality percentage Than meter, consisting of: sawdust accounts for 60-80%, starch accounts for 8-18%, wheat bran accounts for 10-20%, ammonium sulfate 0.1%, peptone 0.1- 0.5%, brown sugar 1.4~1.8%, the sum of each component weight percent is 100%.It cultivates in raw material used in Sparassis crispa, sawdust, Wheat bran is that the agricultural wastes for being easier to obtain have the function of environmental protection using these as raw material.Using this cultivation silk ball In the Sparassis crispa bacteria residue of bacterium remaining waste production, crude protein rich in, amino acid, mycoprotein enzyme, vitamin, mine The nutriments such as prime element also containing nutrients such as N, P, K, while including a large amount of microorganisms and mycelium residual.
It is detailed, it is described from decomposed Sparassis crispa bacteria residue the preparation method comprises the following steps: by after the de- bag crushing of Sparassis crispa bacteria residue, it uses High temperature aerobic composting technology, bacteria residue heap body carry out overlay film stacking by stacking length 2m, width 2m, height 1m, about 4m3, add water tune Bacteria residue heap body water content is saved to 55wt%-65wt%;After heap temperature reaches 50 DEG C or more, remove film, and keep 5-7d Progress turning is primary, and later when temperature reaches 50 DEG C or more, turning again, heap temperature is down to room temperature after 35-40d, embroiders The decomposed completion of coccus bacteria residue;Sparassis crispa bacteria residue after will be decomposed is dried, is crushed and 2mm is spare after sieving excessively, and Sparassis crispa bacteria residue is made.
The rotten agent of any rush is not added in entire digest process from decomposed Sparassis crispa bacteria residue, has effects that health environment-friendly.
Specifically, the physical and chemical index from decomposed Sparassis crispa bacteria residue are as follows: bulk density 0.17-0.19gcm-3, total pore space Spend 83.8-84.9%, aeration porosity 24.1-24.8%, water-holding porosity 59.6-60.1%, organic matter 72-73%, total nitrogen 0.4-0.5%, full phosphorus 0.05-0.06%, full potassium 0.03-0.05%, pH value 6.4-6.5, conductivity 2.1-2.3mScm-1
From physicochemical property as can be seen that having the spy of water-retaining property height, higher, the slightly biased acid of conductivity from decomposed Sparassis crispa bacteria residue Point.In addition, also there is low bulk density from decomposed Sparassis crispa bacteria residue in the case where keeping high porosity, reasonable pH value and conductivity And the characteristics of high organic content.Bulk density is too high to be easy to cause matrix consolidation, and porosity is relatively low, and make moisture in matrix, air, Thermal condition is poor.Organic matter has mineralising, humification, and high organic content has the advantage that 1, is capable of providing work Object nutrient;2, there is water conservation, fertilizer conservation and buffer function;3, promote the formation of crumb structure, improve matrix physical property;4, humic The physiological activity of acid is high;5, the residual hazard and heavy metal pollution of pesticide in matrix is mitigated or eliminated.
A kind of application method according to the above-mentioned cucumber seedling-raising substrate based on Sparassis crispa bacteria residue for raw material, the cucumber Seedling medium uses nutrient solution for cultivating cucumber seedling, seedling raising process, and the formula of the nutrient solution is to add A in every 200L water: Ca(NO3)2For 16kg, KNO3For 6kg;B:KH2PO4For 3kg, MgSO4For 10kg, EDTA-Fe 460g, MnSO4For 120g, ZnSO4For 60g, CuSO4For 4g, (NH4)6Mo7O24·4H2O is 1.5g, H3BO3For 60g.
The cucumber seedling-raising substrate and above-mentioned nutritious liquid prescription use, and the planting percent of cucumber seedling is high, cucumber seedling it is solvable Property sugar, the indexs such as root activity and yield it is best, the cucumber seedling of cultivation is in later period cultivation production test, fruit yield Better than conventional seedbed system formula, increase economic efficiency.
Further to disclose rather than the present invention is limited, the present invention is described in further detail below in conjunction with example.
The embodiment of the present invention uses CK, T1、T2、T3、T4、T5、T6、T7The seedling medium of totally eight kinds of formulas, in the same terms Under seedling growth test data compare, this eight kinds formula seedling mediums it is as shown in Table 1 below.Wherein, T1、T2、T3、T4、T5、 T6、T7For the seedling medium of the bacteria residue containing Sparassis crispa, CK is the control seedling medium without Sparassis crispa bacteria residue.
The composition of eight kinds of formula seedling mediums in 1 present invention of table
Experimental condition: the seedling growth test of eight kinds of formula seedling mediums is in Sino-Israel Model Farm, Fujian Academy of Agricultural Sciences film Greenhouse, glasshouse and bio-chemical characteristics room carry out, and terminate 139 days by a definite date to receipts statistics is produced from nursery is started.By the examination of table 1 It tests design and prepares bacteria residue cucumber seedling-raising substrate, be sufficiently mixed uniformly filling seedling culture hole plate, by seed flat planting in acupuncture point, cover base Matter simultaneously sprinkles profoundly water.Each processing repeats 3 disks, every 72 cave of disk.It is poured after emergence using the uniform nutrient solution of industrial seedling rearing mechanical arm Mode, nutrient solution prescription when nursery are as follows: A:Ca (NO is added in every 200L water3)2For 16kg, KNO3For 6kg;B:KH2PO4For 3kg, MgSO4For 10kg, EDTA-Fe 460g, MnSO4For 120g, ZnSO4For 60g, CuSO4For 4g, (NH4)6Mo7O24·4H2O is 1.5g, H3BO3For 60g.
Bulk density, total porosity, ventilating slit, water holding hole are referring to the saturation extraction of Lian Zhaohuang, and pH measurement is using accurate Acidometer, EC measurement use conductivity meter.Plant height is measured with graduated scale, from rhizome base portion to growing point;Stem thickness vernier Slide calliper rule measure, on the basis of the internode of cotyledon lower part;Leaf area is measured with Leaf area determination instrument, and measurement includes expansion Per a piece of cotyledon and true leaf;Root body volume is measured with drainage;The analysis day that the fresh dry weight of root, stem, leaf is 0.1mg with sensibility reciprocal It is flat to measure;Root/shoot ratio=underground part fresh weight/overground part fresh weight;Seedling vigorous index=(stem thickness/plant height+underground part dry weight/ground Portion's dry weight) × complete stool dry weight.Malonaldehyde (MDA) content is measured using thiobarbituricacidα- method, and chlorophyll content uses 95% second Alcohol extracting is followed the example of, and soluble sugar content is measured using anthrone colorimetry, and content of soluble protein is using the bright method measurement of coomassie, dried meat Propylhomoserin is measured using sulfosalicylic acid method, and root activity is measured using TTC method.Test data is carried out using Excel and DPS software Statistical analysis.
Specific test result is as follows:
1, the determination of physical appearance of the cucumber seedling-raising substrate of eight kinds of formulas is as a result, as shown in Table 2 below.
The physical property of the seedling medium of 2 eight kinds of table formulas
As shown in Table 2, except formula T1Outside, each formulation media bulk density containing Sparassis crispa bacteria residue is all remarkably higher than formula CK, Significant difference.It is formulated T1、T2、T3With the increase of Sparassis crispa bacteria residue content, value of bulk density is in rising trend, is formulated T4、T5、T6Bulk density Value difference is different less, is formulated T7The bulk density of full Sparassis crispa bacteria residue is maximum, significant with each formula variation.In addition to being formulated T4Ventilating slit Lower than formula CK, remaining formula is obviously higher than formula CK;Water holding hole is in addition to being formulated T1Lower than formula CK, remaining formula is high In formula CK.The total porosity being respectively formulated be in 68.93~84.34% in the range of, meet matrix physical property index 54~ 96% ideal range.
2, the chemical property determination of the cucumber seedling-raising substrate of eight kinds of formulas is as a result, as shown in Table 3 below.
The chemical property of the different seedling mediums of table 3
From table 3 it can be seen that the EC value for the cucumber seedling-raising substrate being respectively formulated is below 2.5mS/cm, it is respectively formulated pH value range Between 6.40-6.85, meet the appropriate pH of Vegetable culture medium: 6.0~7.5.Except formula T1Outside, containing Sparassis crispa bacteria residue The content of organic matter of each formulation media be all remarkably higher than formula CK, and the total nitrogen, full phosphorus, full potassium content that are formulated CK are significantly higher than Remaining formula.It is formulated T4、T5、T6With the increase of Sparassis crispa bacteria residue content, the content of organic matter is increased, and total nitrogen, full phosphorus, full potassium contain Amount decline, is formulated T7The content of organic matter highest of full Sparassis crispa bacteria residue, there are significant differences with remaining formula, and full phosphorus, complete Potassium content is lower than formula CK and remaining formula.
3, influence of the cucumber seedling-raising substrate of eight kinds of formulas to cucumber seedling emergence rate, in detail as shown in Figure 1.
It will be seen from figure 1 that after planting 5d, the corresponding cucumber emergence rate of the cucumber seedling-raising substrate of all formulas have been more than 95%, illustrate that each matrix formulations are able to satisfy the needs of seedling sprouting.Wherein emergence rate highest formula T4, T6 is more than formula CK reaches 100%, and formula T5 emergence rate is identical as formula CK, and is formulated T1、T2、T3、T7Emergence rate is lower than formula CK.Illustrate to embroider Coccus bacteria residue cannot replace turf completely, and after a certain amount of turf is added, cucumber seedling effect is more preferably.
4, influence of the cucumber seedling-raising substrate of eight kinds of formulas to cucumber seedling growth and development, it is as shown in Table 4 below.
Influence of the cucumber seedling-raising substrate of 4 eight kinds of table formulas to cucumber seedling growth and development
Table 4 is the growth indexes dependency number of different number of days after the cucumber emergence that the cucumber seedling-raising substrate of different formulations is cultivated According to.10d and 15d after emergence is behaved oneself best with being formulated CK in terms of plant height, plumular axis length, stem thickness, is formulated T after emergence7Performance It is worst;The root long of 10d, root/shoot ratio and seedling vigorous index are to be formulated T after emergence1Behave oneself best;15d after emergence, root long is to be formulated T2 Show optimal, root/shoot ratio is to be formulated T3It puts up the best performance, seedling vigorous index is still formula T1Behave oneself best;20d after emergence is formulated T5's Plant height is significantly higher than remaining formula, and the plumular axis for being formulated CK is long maximum, but with formula T4、T5Difference is not significant;Stem thickness, root long are to match Square T1Behave oneself best, root/shoot ratio, seedling vigorous index are to be formulated T2It shows optimal.
5, influence of the cucumber seedling-raising substrate of eight kinds of formulas to cucumber seedling root volume, in detail as shown in Figure 2.Eight kinds formula Influence of the cucumber seedling-raising substrate to cucumber seedling leaf area, in detail as shown in Figure 3.
Figure it is seen that the 20d after emergence, is formulated T2Root volume it is maximum, and be noticeably greater than formula CK and formula T4、 T5, formula CK is minimum, but with formula T4、T5Difference is not significant, is formulated T6、T7Root volume is close.The root volume size of cucumber seedling It is followed successively by T2> T3> T7> T6> T1> T4> T5> CK.
From figure 3, it can be seen that the 20d after emergence, is formulated T5Leaf area it is maximum, with formula CK, T1、T2Significant difference.It is yellow The leaf area size of melon seedling is successively T5> T4> T3> T6> T7> T2> T1> CK.
In summary cucumber seedling growth index thinks, adds stem thickness, root volume, leaf of the Sparassis crispa bacteria residue to cucumber seedling Area and root/shoot ratio have facilitation.
6, influence of the cucumber seedling-raising substrate of eight kinds of formulas to cucumber seedling physical signs, see Table 5 for details shown in content.
Influence of the cucumber seedling-raising substrate of 5 eight kinds of table formulas to cucumber seedling physical signs
By table 5 it can be concluded that, during cucumber seedling growth, soluble sugar content in blade exists fall before after on The trend risen.10d after emergence is formulated T4Soluble sugar content highest, with formula CK, T7Difference is not significant, with remaining formula variation Significantly;15d after emergence, each formulation soluble sugared content decline, formula CK soluble sugar content are significantly higher than remaining formula;Emergence 20d afterwards, formula CK soluble sugar content still show as declining, remaining formulation soluble sugared content increases, and is formulated T4Soluble sugar Content is significantly higher than remaining formula, is embodied in T4> T1> T6> T7> T3> CK > T5> T2.Soluble sugar is as intermediate The growth, development and resistance that product takes part in plant such as form at the processes, and the controllable blade and blade of soluble sugar in plant leaf is green Cellulose content influences intensity of photosynthesis.Test result shows to be formulated T4Soluble sugar content highest illustrates the photosynthetic work of the formula It is strong with ability, cucumber seedling growth can be promoted.
With the growth of cucumber seedling, soluble protein is gradually increased.After emergence between 10d to 15d, each formulation soluble Property protein content accumulation it is less, 15 between 20d after emergence, and soluble protein content accumulation is more.10d and emergence after emergence 15d afterwards, the soluble protein content for being formulated CK are higher than remaining seedling medium formula.20d after emergence is formulated T3Soluble protein contains Highest is measured, with formula T1、T5、T7Significant difference, it is not significant with remaining formula variation, and it is formulated T1、T5、T7Soluble protein content Lower than formula CK.Except T1、T5、T7Outside, generally with the increase of Sparassis crispa bacteria residue usage amount, content of soluble protein is therewith Increase, specific manifestation are as follows: T3> T2 > T6> T4> CK > T7> T5> T1.Soluble protein can participate in a variety of metabolism and live Dynamic, soluble protein content is the important indicator for measuring seedling metabolism degree in blade.Compare formula T2、T3、T4、T6It was found that can Dissolubility protein content increases as Sparassis crispa bacteria residue usage amount increases, and illustrates that use ratio is not higher than 75% Sparassis crispa bacterium Slag can promote the accumulation of cucumber seedling soluble protein;But when bacteria residue usage amount is 100%, soluble protein is instead Decline, illustrates that 100% Sparassis crispa bacteria residue is unfavorable for the accumulation of soluble protein.
During cucumber growth, formula CK chlorophyll content is continued to decline with the growth of cucumber seedling, remaining formula Chlorophyll content shows as rising after falling before.10d after emergence, formula CK chlorophyll content are significantly higher than remaining formula, secondly It is formula T3.15d after emergence, formula T4 chlorophyll content are higher than formula CK, but not significant with formula CK difference.20d after emergence, The chlorophyll content of cucumber seedling-raising substrate formula is above formula CK, is formulated T5Chlorophyll content highest, but with formula T6、T7Nothing Significant difference.The content size of seedling Determination of Chlorophyll is successively T5> T7> T6> T3> T4> T1> T2> CK.Chlorophyll conduct Main photosynthetic pigments participate in the photosynthesis of plant, reflect the photosynthetic capacity of plant.Experiments have shown that formula T5Chlorophyll contain Highest is measured, the chlorophyll content of remaining formula is above formula CK.
During entire cucumber seedling-raising, proline content shows the trend of rising.10d after emergence, T5Proline contains Highest is measured, formula CK content is minimum;15d after emergence is formulated T1、T2Proline content be higher than formula CK, remaining formula is below It is formulated CK;20d after emergence is formulated T2Proline content be significantly higher than formula CK, with formula T1、T5Difference is not significant, with remaining Formula variation is significant.In whole process, it is formulated T2Proline content rise most fast, aggregate performance T2> T5> T1> T3> T6 > CK > T7> T4.Proline is important one of osmotic adjustment, and it is degeneration-resistant that the intracorporal proline content of plant can reflect its Property degree.In this test, it is formulated T1、T2、T3、T5、T6Proline content be higher than formula CK proline content, be formulated T4、T7 Proline content lower than formula CK proline content, illustrate matrix of the Sparassis crispa bacteria residue use ratio between 50~75% Formula is conducive to Leaf of Cucumber Seedling accumulation proline.
10d after cucumber emergence, is formulated T2、T4MDA content be significantly higher than formula CK and remaining formula;15d after emergence, matches Square T5MDA content increase it is most fast, be significantly higher than each formula;20d after emergence is formulated CK, T1、T4、T5MDA content decline, It is formulated T2、T3、T6、T7MDA content increase, be formulated T1MDA content lower than formula CK MDA content, but with formula CK difference It is not significant.MDA content size in seedling is successively T7> T3> T4> T2> T6> T5> CK > T1.MDA is as plant body fat Matter peroxidating index indicates the power of Genes For Plant Tolerance adverse circumstance ability.Control formula CK and formula T in this test1MDA content most Low, the resistance of seedling is most strong, is formulated T7MDA content highest, the resistance of seedling is most weak.
10d after cucumber emergence, is formulated T1Root activity is significantly higher than formula CK, remaining formula vigor, which is substantially less than, to be formulated CK is formulated T4Root activity is minimum;15d after emergence is formulated T4、T7Root activity shows as increasing, remaining formula vigor shows For decline, it is formulated CK root activity highest, is formulated T1Take second place, the two difference is not significant;20d after emergence is formulated T1, CK root system it is living Power continues to decline, remaining formula root activity increases, and is formulated T4Root activity is significantly higher than remaining formula, is embodied in T4> T6> T7> T5> T2> T1> T3> CK.Root activity is that the synthesis of the growth for measuring plant root, metabolism and absorption function refers to Mark, is able to reflect the growth and development in plant above ground portion.The root activity of bacteria residue cucumber seedling-raising substrate is above control in this test Turf cucumber seedling-raising substrate is formulated T4Root activity highest, illustrate the tetrazole reduction intensity highest of the seedlings root.
In conclusion Sparassis crispa bacteria residue: turf: perlite=1: 1: 1 soluble sugar content, root activity index are better than Remaining formula, Sparassis crispa bacteria residue optimal use ratio are 33%.
7, influence of the cucumber seedling-raising substrate of eight kinds of formulas to yield after cucumber cultivation
The end of nursery after sowing one month, is colonized in glasshouse coco bran matrix strips for the cucumber seedling obtained after nursery In, test is cultivated after carrying out nursery.During cultivated cucumber, the nutrient solution prescription that uses are as follows: add A in every 100L water: MgSO4For 7.36kg, KH2PO4For 3.2kg, H3BO3For 45.76g, MnSO4For 25.83g, ZnSO4For 3.5g, CuSO4For 1.28g NH4)6Mo7O24·4H2O is 0.32g;B:Ca (NO3)2For 11.8kg, KNO3For 6.56kg, EDTA-Fe 320g.
Influence of the cucumber seedling-raising substrate of 6 eight kinds of table formulas to cucumber yield
Table 6 is the cucumber yield harvested in -139 days 57 days after nursery.As can be seen from the table, single plant fruit number and yield Decline with the increase of Sparassis crispa bacteria residue use ratio, is formulated T4Single fruit number is significantly higher than remaining formula, is formulated T7Single fruit digital display writes Lower than remaining formula, single fruit number size is followed successively by T4> T1> CK > T5> T6> T3> T2> T7.It is formulated T2、T5、T7Single fruit weight It is all larger than formula CK, but all not significant with formula CK difference, is embodied in T5> T2> T7> CK > T6> T4> T3> T1.It is single Every 667m after strain yield and conversion2Yield is to be formulated T4、T5Performance preferably, is significantly higher than formula CK and remaining formula, formula CK、T1、T2Single plant yield and every 667m2Volume variance is not significant, is formulated T7Yield is minimum, and there are conspicuousnesses with each formula Difference.Yield size is followed successively by T4> T5> CK > T1> T2> T6> T3> T7
As known from Table 6, it is formulated T2It is showed preferably in root/shoot ratio, the seedling vigorous index of nursery stage, better than formula T4、T5, after cultivation Yield is inferior to formula T4、T5.It is formulated T4In single fruit number, single plant yield and every 667m2It puts up the best performance in terms of yield, is formulated T5Yield Take second place, is superior to compare.Illustrate that the ratio of Sparassis crispa bacteria residue part substitution turf is conducive to improve cucumber between 33~50% Yield, substitute the ratio of turf completely at 50~66%, yield is approached with compare, and the yield of Sparassis crispa bacteria residue is most entirely It is low.
From the foregoing, it will be observed that when Sparassis crispa bacteria residue: turf: when the volume ratio of perlite is 1: 1: 1, the cucumber seedling of cultivation is in single plant It is superior to remaining formula in terms of fruit number, yield, therefore, Sparassis crispa bacteria residue optimal use ratio is 33%.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to this patent range of the present invention and dress Decorations, all should belong to covering scope of the invention.

Claims (9)

1. it is a kind of based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate, which is characterized in that the cucumber seedling-raising substrate by It is formed from decomposed Sparassis crispa bacteria residue, perlite and turf, is 1: 1 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf: 1~3: 1: 1.
2. it is according to claim 1 based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate, which is characterized in that it is described It is 1: 1: 1 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf in cucumber seedling-raising substrate.
3. it is according to claim 1 based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate, which is characterized in that it is described It is 2: 1: 1 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf in cucumber seedling-raising substrate.
4. it is according to claim 1 based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate, which is characterized in that it is described It is 3: 1: 1 from the volume ratio of decomposed Sparassis crispa bacteria residue, perlite and turf in cucumber seedling-raising substrate.
5. it is according to claim 1 based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate, which is characterized in that it is described In cucumber seedling-raising substrate, from the partial size of decomposed Sparassis crispa bacteria residue are as follows: 2mm.
6. it is according to claim 1 based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate, which is characterized in that it is described from The raw material of decomposed Sparassis crispa bacteria residue is the cultivation remaining waste of Sparassis crispa, by mass percentage, consisting of: sawdust accounts for 60-80%, starch account for 8-18%, wheat bran accounts for 10-20%, ammonium sulfate 0.1%, peptone 0.1-0.5%, and brown sugar 1.4~ 1.8%, the sum of each component weight percent is 100%.
7. it is according to claim 6 based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate, which is characterized in that it is described After from decomposed Sparassis crispa bacteria residue the preparation method comprises the following steps: Sparassis crispa bacteria residue to be taken off to bag crushing, using high temperature aerobic composting technology, bacteria residue Heap body presses stacking length 2m, width 2m, height 1m, about 4m3Carry out overlay film stacking, add water adjust bacteria residue heap body water content to 55wt%-65wt%;After heap temperature reaches 50 DEG C or more, remove film, and keep 5-7d progress turning primary, works as later When temperature reaches 50 DEG C or more, turning again, heap temperature is down to room temperature, the decomposed completion of Sparassis crispa bacteria residue after 35-40d;It will Sparassis crispa bacteria residue after decomposed is dried, is crushed and 2mm is spare after sieving excessively, and Sparassis crispa bacteria residue is made.
8. it is according to claim 7 based on Sparassis crispa bacteria residue be raw material cucumber seedling-raising substrate, which is characterized in that it is described From the physical and chemical index of decomposed Sparassis crispa bacteria residue are as follows: bulk density 0.17-0.19gcm-3, total porosity 83.8-84.9%, ventilating slit Spend 24.1-24.8%, water-holding porosity 59.6-60.1%, organic matter 72-73%, total nitrogen 0.4-0.5%, full phosphorus 0.05- 0.06%, full potassium 0.03-0.05%, pH value 6.4-6.5, conductivity 2.1-2.3mScm-1
9. a kind of based on being made described in claim 1-8 any one based on the cucumber seedling-raising substrate that Sparassis crispa bacteria residue is raw material With method, which is characterized in that the cucumber seedling-raising substrate uses nutrient solution, the battalion for cultivating cucumber seedling, seedling raising process The formula of nutrient solution is that A:Ca (NO is added in every 200L water3)2For 16kg, KNO3For 6kg;B:KH2PO4For 3kg, MgSO4For 10kg, EDTA-Fe is 460g, MnSO4For 120g, ZnSO4For 60g, CuSO4For 4g, (NH4)6Mo7O24·4H2O is 1.5g, H3BO3For 60g。
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CN110972895A (en) * 2019-12-10 2020-04-10 太仓绿丰农业资源开发有限公司 Novel seedling culture substrate for resisting cucumber downy mildew
CN112841190A (en) * 2021-01-29 2021-05-28 福州市蔬菜科学研究所 Coating agent for cruciferous vegetable seeds and preparation method and using method thereof
CN114271172A (en) * 2021-03-30 2022-04-05 福州市蔬菜科学研究所 Tomato composite matrix formula
CN114303881A (en) * 2021-03-30 2022-04-12 福州市蔬菜科学研究所 Tomato and eggplant seedling culture substrate taking sparassis crispa residues as main material

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CN109452128A (en) * 2018-12-13 2019-03-12 山东省农业科学院农业资源与环境研究所 A kind of preparation method of the cucumber soilless raising seedling substrate based on Uricularia polytricha bacteria residue

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CN106069675A (en) * 2016-06-24 2016-11-09 广东森维绿联科技有限公司 A kind of leaf vegetables plant soil-less culturing substrate and cultural method thereof
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CN110972895A (en) * 2019-12-10 2020-04-10 太仓绿丰农业资源开发有限公司 Novel seedling culture substrate for resisting cucumber downy mildew
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