CN1631078A - Wild-imitating mushroom edible fungus seed and cultivation method thereof - Google Patents
Wild-imitating mushroom edible fungus seed and cultivation method thereof Download PDFInfo
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Abstract
The invention relates to a wild-imitating mushroom edible fungus seed and cultivation method, wherein the first batch of separate original bacterial are collected, and tissue disconnection or spore segregation are carried out to cultivate the original bacterial on the stock culture media to obtain the stock culture. It only takes 20-30 days before the stock culture can be ready, and can be applied to the first, second and third level bacterial transferring expansion or cultivation directly, the second level bacterial culturing cycle can be shortened substantially. The bacterial are preserved in China General Microbiological Culture Collection Center, with the collection number being CGMCC1068.
Description
The present invention relates to a kind of imitative wild mushroom class edible bacterium and cultural method, the Mycophyta of microorganism belonging to genus, this bacterial classification are applicable to mushroom class edible mushroom and medicinal fungus such as cultivating cultivating ganoderma, mushroom, hedgehog hydnum.
Mushroom class edible mushroom major parts such as China glossy ganoderma, hedgehog hydnum, mushroom are artificial cultivations, and wild bacterium is fewer and feweri; The promptly female kind of the wild juggle bacterium of the Nature breeds and needed for 1～2 year, and the secondary original seed needs 20～30 days, and three-class strain needs 1～2 month; General female plant under 2～6 ℃ of environment about 2 months just aging, can only change usually and expand 3～5 times, change and expand manyly more, spawn degeneration is serious more, kind property is poor, yields poorly, cultivation cycle is long, therefore need select a kind of imitative wild edible bacterial classification and cultural method newly.
The objective of the invention is in order to protect forest resources; overcome the deficiency that existing artificial breeding technique exists; a kind of new imitative wild mushroom class edible bacterium and this culture of strains method are provided; this bacterial classification shortened greatly from the cultivation time that mother plants three-class strain; it is good to plant property; female kind preservation time is long, and female kind can be used for the commentaries on classics expansion of one, two, three bacterial classification or directly cultivates.
The objective of the invention is to reach by following measure:
Should imitate wild mushroom class edible bacterium, be to gather first batch of only original strain, by separate tissue or spore separation method, tissue isolation is to get piece of tissue in only stalk entity capsule, the spore separation method is to get fruit body lamella piece of tissue, this isolated original strain cultivated on mother culture media and obtain mother's kind of imitative wild mushroom class edible bacterium, it is characterized in that:
A) on mother culture media, mycelia is pure white pure strong, is the fine hair shape, and dense material feeding is fast, and 24～27 ℃ of cultivations, 20～sky covers with the full bottle of 750g wide-mouth bottle;
B) mycelia suits to grow on the meta-alkalescence medium, pH value 6.0～7.5;
C) after mother plants long getting well, can be applicable to the commentaries on classics expansion of one, two, three bacterial classification or directly apply to cultivation;
D) this mother plants and can not degenerate more than 3 years storing in 2～5 ℃ under the sealing of paraffin;
E) the anti-assorted bacterium of mycelia, mushroom class sheets such as its fruit body mushroom, hedgehog hydnum, glossy ganoderma are big and plump, utilize this mother to plant the cultivation original seed and only need 5～8 days, cultivate three-class strain and need 10～18 days;
This culture presevation is at China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is: CGMCC1068.Preservation date is: on December 11st, 2003.
The cultural method of the imitative wild mushroom class edible bacterium of the present invention is that a) the above-mentioned preserving number of employing is the bacterial classification of CGMCC1068; B) preparation of first class inoculum and medium is:
Culture medium raw material prescription and proportioning:
The fruit tree branch of diameter 1.2～1.8cm, wild branch or its mixed branch 75～85%, millet powder: corn flour: analysis for soybean powder=1: 1.2: 0.8 wherein a kind of or its mixture 10～18%, brown sugar 2～6%, quicklime 0.8～1.2%, gypsum 0.8～1.5%, composite fertilizer 0.8～1.5%, composite fertilizer select phosphate fertilizer, fused calcium magnesium phosphate, setting sun board composite fertilizer etc. for use, 0.02% mushroom is strong plain, and clear water is standby;
Compound method:
Culture medium raw material is put into clear water make soak, down soaked branches 2～4 days with this soak in 15～30 ℃, it is standby in the wide-mouth bottle with humidity to be that 60～68% soaked branch sections are packed into again; Getting 32～40% wood chip mixes with magma at the bottom of 60～68% the immersion, the wood chip spice is placed compacting on bottle interior branch section, wood chip thickness is 1～2 centimeter, the capping bottleneck places in the pressure cooker and sterilized 1.5～2 hours, be cooled to 30 ± 2 ℃ of taking-up cartonnings and connect original strain, put under 24～27 ℃ of isoperibols and cultivated 20～30 days, mycelia is covered with full bottle.
When mother plants the need preservation, melted paraffin wax can be potted in the wood chip charge level in the inoculation bottle, general every bottle adds 2～3 little spoons, is as the criterion to seal the wood chip charge level, uses Polypropylence Sheet capping bottleneck again, and this mother plants and can preservation not degenerate more than 3 years under 2～5 ℃ of temperature; When needing, only need open to seal with wax and just can use with female the kind.
The present invention can also realize by following technical scheme:
The preparation method of second class inoculum and medium is: place the aqueous solution that contains 1～6% quicklime and the strong element of 0.02% mushroom to soak 2～3 days iblet, then the iblet rinsing was gone into pan boiling for 2～3 times 10～15 minutes, use water rinse 3～5 times; Again iblet was dried in the air 12～20 hours in the moon place, iblet that dries well and land plaster are mixed bottling thoroughly in 100: 0.8 ratio, pressure cooker sterilization 1.5～2hr is inserted in the bottleneck capping, takes out kind of a bottle cartonning inoculation when being cooled to 30 ± 2 ℃, cultivates 5～8 days mycelia down in 24～27 ℃ and covers with full bottle.
Three-class strain and cultural method are:
A) culture medium prescription and proportioning:
55～70% cotton seed hullss, 20～30% corncob, the mixture of wood chip or its are a kind of, and the mixture of 5～15% wheat bran, rice bran, corn flour, jowar face or its are a kind of, gypsum 1～1.5%, composite fertilizer 1～1.5%, lime 1～1.5%, mushroom strong plain 0.02%;
B) compound method:
Culture medium raw material is mixed; add the iblet that dries well with the lime water processing in 1/5～1/2 ratio; jowar grain or wheat; it is strong plain to add mushroom in the batch mixing; making the strong plain content in compound of mushroom is 0.02%; add the water spice; make its water content reach 65～68%, in the plastic sack of more moisture spice being packed into, bag end cover non-cotton cover; go into pressure cooker sterilization 1.5～2hr; when waiting to be cooled to 30 ± 2 ℃, in the punching inoculation of bag body middle part, hole depth is as the criterion not penetrate; fill up bacterial classification rear enclosed aperture in the hole; non-cotton cover is overlapped in two spoonfuls of inoculations of binding of two ends one side of bag then, cultivates down in 24～27 ℃ and can cover with the bacterium bag in 10～18 days.
The present invention has following advantage:
1, sturdy, the anti-assorted bacterium of mycelia, eat assorted bacterium, mycelia heatproof, cold-resistant;
2, female all property are good, and the preservation time is long, and can be used for the commentaries on classics expansion or the directly cultivation of one, two, three bacterial classification, and the cultivation time of planting three-class strain from mother shortens greatly, shortens 2～3 times cultivation time than liquid spawn;
3, entity output height, the medium source is wide, cost is low, and cultivation method is easy to promote operation.
Below by embodiment the present invention is described in detail.
Embodiment 1:
First class inoculum culture medium prescription (by weight)
Fruit tree branch 78kg or wild branch (mountain hazel, the twigs of the chaste tree, bitter leaf tree) 78kg
Corn flour: millet powder: analysis for soybean powder=1.2: 1: 0.8 common 16kg
Brown sugar 3kg
Gypsum 1kg
Fused calcium magnesium phosphate 1kg
Quicklime 1kg
Mushroom is strengthened plain 20g
The preparation cultural method:
Above-mentioned culture medium raw material being put into clear water make soak, soaked branch 2～4 days with this soak down in 20～30 ℃, do not have branch to be advisable with soak, is standby in 60～68% soaked branch segment dress wide-mouth bottles again with humidity; Get 35% the wood chip that sieves with 65% immersion the end magma of branch mix and mix thoroughly, the wood chip spice is placed compacting on bottle interior branch section, wood chip thickness is 1～2 centimeter, bottleneck seals with one deck brown paper one deck Polypropylence Sheet, go into pressure cooker in the 2hr that sterilizes more than 100 ℃, be cooled to 30 ± 2 ℃ of taking-up cartonnings and connect the piece of tissue that original strain promptly separates, put under 24～27 ℃ of isoperibols and cultivated 20～30 days, mycelia is covered with full bottle.Female plant long good after, can be applicable to one, two, three bacterial classification changes and expands, and can also directly cultivate fruiting with female the kind; When mother plants when needing preservation, need do sterile working at inoculating hood or superclean bench, the paraffin of fusing is potted in wood chip charge level in the inoculation bottle, use Polypropylence Sheet capping bottleneck again, this mother plants and can preservation not degenerate more than 3 years under 2～5 ℃ of temperature.
Embodiment 2:
Preparation of second class inoculum medium and cultural method:
Place the aqueous solution that contains 5% quicklime and the strong element of 0.02% mushroom to soak 2～3 days the iblet of full seed, then iblet being gone into a pot fire for 2～3 times with water rinse boiled 10～15 minutes, not having living core with iblet is advisable, then iblet is pulled in the clear water out rinsing 3～5 times, iblet being placed on cloudy place dried in the air 12～20 hours again, iblet does not have percentage of damage will reach 95～100%, mix iblet and gypsum thoroughly bottling in 100: 0.8 ratio then, and be contained under the shoulder, pressure cooker sterilization 1.5～2 hours is inserted in the bottleneck capping, takes out kind of a bottle cartonning inoculation when being cooled to 30 ± 2 ℃ naturally; The female kind switching of wooden unit is that the female kind of long good branch section is inserted in the second class inoculum medium, every bottle of equidistant four blocks of wood ferfas kinds of inserting, wood branch block length equates that with iblet height in the bottle cultivated 5～8 days the inoculation back, can cover with mycelia in the bottle under 24～27 ℃ of temperature.
Embodiment 3:
Three-class strain and cultural method:
A) culture medium prescription and proportioning:
61.98% cotton seed hulls, 25% corncob and wood chip (corncob: wood chip=1: 1)
10% wheat bran and corn flour (wheat bran: corn flour=1: 1), gypsum 1%, phosphate fertilizer 1%, lime 1%, 0.02% mushroom is strong plain.
B) preparation cultural method:
Culture medium raw material is mixed the back add the iblet (medium compound 60%) that the processing of 40% usefulness lime water dries well; It is strong plain to add mushroom then in compound, makes its content in compound reach 0.02%, adds the water spice, makes the spice water content reach 65～68%, piles vexed diel; Again moisture spice is packed in the plastic sack, bag end cover non-cotton cover, go into pressure cooker sterilization 1.5～2 hours, when waiting to reduce to 30 ± 2 ℃, in the punching inoculation of bag body middle part, hole depth is as the criterion not penetrate, fill up bacterial classification rear enclosed aperture in the hole, non-cotton cover is overlapped in two spoonfuls of inoculations of binding of two ends one side of bag then, the bacterium bag is pressed # font discharging 4～6 floor heights, ventilates every day 1～2 hour.Cultivated 10～18 days down in 24～27 ℃, can cover with the bacterium bag.
Claims (6)
1, imitative wild mushroom class edible bacterium is to gather first batch of only original strain, by separate tissue or spore separation method, this isolated original strain is cultivated on mother culture media and mother's kind of obtaining imitating wild mushroom class edible bacterium, it is characterized in that:
A) on mother culture media, mycelia is pure white pure strong, is the fine hair shape, and dense material feeding is fast, 24～27 ℃ of cultivations, covers with the full bottle of 750g wide-mouth bottle in 20～30 days;
B) mycelia suits to grow on the meta-alkalescence medium, pH value 6.0～7.5;
C) after mother plants long getting well, can be applicable to the commentaries on classics expansion of one, two, three bacterial classification or directly apply to cultivation;
D) this mother plants and can not degenerate more than 3 years storing in 2～5 ℃ under the sealing of paraffin;
E) the anti-assorted bacterium of mycelia, mushroom class sheets such as its fruit body mushroom, hedgehog hydnum, glossy ganoderma are big and plump, utilize this mother to plant the cultivation original seed and only need 5～8 days, cultivate three-class strain and need 10～18 days;
This culture presevation is at China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is: CGMCC1068.
2, the cultural method of imitative wild mushroom class edible bacterium is characterized in that:
A) bacterial classification is the described bacterial classification of claim 1;
B) preparation of first class inoculum and medium
Culture medium raw material prescription and proportioning:
The fruit tree branch of diameter 1.2～1.8cm, wild branch or its mixed branch 75～85%, millet powder: corn flour: analysis for soybean powder=1: 1.2: 0.8 wherein a kind of or its mixture 10～18%, brown sugar 2～6%, quicklime 0.8～1.2%, gypsum 0.8～1.5%, composite fertilizer 0.8～1.5%, 0.02% mushroom is strong plain, and clear water is standby;
Compound method:
Culture medium raw material is put into clear water make soak, down soaked branches 2～4 days with this soak in 15～30 ℃, it is standby in the wide-mouth bottle with humidity to be that 60～68% soaked branch sections are packed into again; Getting 32～40% wood chip mixes with magma at the bottom of 60～68% the immersion, the wood chip spice is placed compacting on bottle interior branch section, wood chip thickness is 1～2 centimeter, the capping bottleneck places sterilization in the pressure cooker, be cooled to 30 ± 2 ℃ of taking-up cartonnings and connect original strain, put under 24～27 ℃ of isoperibols and cultivated 20～30 days, mycelia is covered with full bottle.
3, according to the described cultural method of claim 2, it is characterized in that the paraffin that will melt is potted in the wood chip charge level in the inoculation bottle, use Polypropylence Sheet capping bottleneck again, this mother plants and can preservation not degenerate more than 3 years under 2～5 ℃ of temperature.
4, according to the described cultural method of claim 2, the preparation method who it is characterized in that second class inoculum and medium is: place the aqueous solution that contains 1～6% quicklime and the strong element of 0.02% mushroom to soak 2～3 days iblet, then the iblet rinsing was gone into pan boiling for 2～3 times 10～15 minutes, use water rinse 3～5 times after taking the dish out of the pot again, again iblet was dried in the air 12～20 hours in the moon place, iblet that dries well and land plaster are mixed bottling thoroughly in 100: 0.8 ratio, pressure cooker sterilization 1.5～2hr is inserted in the bottleneck capping, take out kind of a bottle cartonning inoculation when being cooled to 30 ± 2 ℃, cultivate 5～8 days full bottles down in 24～27 ℃.
5, according to the described cultural method of claim 4, the inoculation method that it is characterized in that making second class inoculum is to adopt that wooden unit is female plants switching, be that female kind of long good branch section inserted in the second class inoculum medium, every bottle of equidistant four ferfas kinds wood branch piece of inserting, the length of wood branch piece equates with the height of maize culture medium.
6,, it is characterized in that three-class strain and cultural method are according to the described cultural method of claim 2:
A) culture medium prescription and proportioning:
55～70% cotton seed hullss, 20～30% corncob, the mixture of wood chip or its are a kind of, and the mixture of 5～15% wheat bran, rice bran, corn flour, jowar face or its are a kind of, gypsum 1～1.5%, composite fertilizer 1～1.5%, lime 1～1.5%, mushroom strong plain 0.02%;
B) compound method:
Culture medium raw material is mixed, add in 1/5～1/2 ratio and handle iblet, jowar grain or the wheat that dries well; It is strong plain to add mushroom in the compound, and making the strong plain content in compound of mushroom is 0.02%, adds the water spice, make its water content reach 65～68%, in the plastic sack of again spice being packed into, bag end cover non-cotton cover, go into pressure cooker sterilization 1.5～2hr, when waiting to be cooled to 30 ± 2 ℃, in the punching inoculation of bag body middle part, hole depth is as the criterion not penetrate, fill up bacterial classification rear enclosed aperture in the hole, non-cotton cover is overlapped in two spoonfuls of inoculations of binding of two ends one side of bag then, cultivates down in 24～27 ℃ and can cover with the bacterium bag in 10～18 days.
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Cited By (17)
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| CN102487727A (en) * | 2011-12-26 | 2012-06-13 | 湖南省春华生物科技有限公司 | Method for producing pleurotus eryngii quel strains |
| CN102690141A (en) * | 2012-01-15 | 2012-09-26 | 河南科技大学 | Black fungus culture medium and preparation method thereof, and method for ensuring original strain inoculation survival percent of black fungus |
| CN103004474A (en) * | 2012-12-31 | 2013-04-03 | 陕西众兴菌业科技有限公司 | Method for solving problem of bacterial variation degradation of liquid-strain nutrition liquid |
| CN103238459A (en) * | 2012-02-14 | 2013-08-14 | 贵州省生物研究所 | Breeding method of multi-spore lingzhi mushroom |
| CN103355101A (en) * | 2013-07-27 | 2013-10-23 | 何寒 | Lucid ganoderma stock culture and preparation method thereof |
| CN103435391A (en) * | 2013-08-21 | 2013-12-11 | 福建省农业科学院食用菌研究所 | Optimized formula and preparation process for W2000 liquid stock of mushroom breathing bag cultivated species |
| CN103688758A (en) * | 2013-12-15 | 2014-04-02 | 张爱美 | Original ecological cultivation method for trametes |
| CN104303845A (en) * | 2014-11-03 | 2015-01-28 | 河北大学 | Method for cultivating lucid ganoderma through thorns crumbs |
| CN104311257A (en) * | 2014-09-30 | 2015-01-28 | 汤阴县食用菌研究所 | Mother strain culture medium formula of wood-rotting edible fungi and preparation method of mother strain culture medium |
| CN104429591A (en) * | 2014-11-03 | 2015-03-25 | 河北大学 | Method for cultivating auricularia polytricha through thorn scraps |
| CN104478547A (en) * | 2014-11-03 | 2015-04-01 | 河北大学 | Method for utilizing vitex negundo var ineica scrap to culture pholiota adiposa |
| CN105191666A (en) * | 2015-09-24 | 2015-12-30 | 榕江县苗岭菌类种植加工厂 | Method for imitating wild cultivation of ganoderma atrum |
| CN106576896A (en) * | 2016-11-22 | 2017-04-26 | 曹晓龙 | Method for preparing stock seeds of high-mountain ganoderma lucidum |
| CN106665124A (en) * | 2017-01-18 | 2017-05-17 | 贵州省山地资源研究所有限公司 | Cultivation method for returning coriaria sinia mushroom to nature |
| CN108617410A (en) * | 2018-08-13 | 2018-10-09 | 轩先森 | A kind of culture medium of edible fungus material and the preparation method and application thereof made by hazel material |
| CN109006163A (en) * | 2018-07-03 | 2018-12-18 | 沈阳农业大学 | A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method |
| CN111602557A (en) * | 2020-06-23 | 2020-09-01 | 常德市永春堂生物科技有限公司 | Lucid ganoderma cultivation method |
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2003
- 2003-12-23 CN CN 200310119185 patent/CN1631078A/en active Pending
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| CN102487727A (en) * | 2011-12-26 | 2012-06-13 | 湖南省春华生物科技有限公司 | Method for producing pleurotus eryngii quel strains |
| CN102690141A (en) * | 2012-01-15 | 2012-09-26 | 河南科技大学 | Black fungus culture medium and preparation method thereof, and method for ensuring original strain inoculation survival percent of black fungus |
| CN102690141B (en) * | 2012-01-15 | 2013-12-25 | 河南科技大学 | Black fungus culture medium and preparation method thereof, and method for ensuring original strain inoculation survival percent of black fungus |
| CN103238459A (en) * | 2012-02-14 | 2013-08-14 | 贵州省生物研究所 | Breeding method of multi-spore lingzhi mushroom |
| CN103004474A (en) * | 2012-12-31 | 2013-04-03 | 陕西众兴菌业科技有限公司 | Method for solving problem of bacterial variation degradation of liquid-strain nutrition liquid |
| CN103004474B (en) * | 2012-12-31 | 2013-11-27 | 陕西众兴菌业科技有限公司 | Method for solving problem of bacterial variation degradation of liquid-strain nutrition liquid |
| CN103355101A (en) * | 2013-07-27 | 2013-10-23 | 何寒 | Lucid ganoderma stock culture and preparation method thereof |
| CN103435391A (en) * | 2013-08-21 | 2013-12-11 | 福建省农业科学院食用菌研究所 | Optimized formula and preparation process for W2000 liquid stock of mushroom breathing bag cultivated species |
| CN103688758A (en) * | 2013-12-15 | 2014-04-02 | 张爱美 | Original ecological cultivation method for trametes |
| CN104311257A (en) * | 2014-09-30 | 2015-01-28 | 汤阴县食用菌研究所 | Mother strain culture medium formula of wood-rotting edible fungi and preparation method of mother strain culture medium |
| CN104303845A (en) * | 2014-11-03 | 2015-01-28 | 河北大学 | Method for cultivating lucid ganoderma through thorns crumbs |
| CN104429591A (en) * | 2014-11-03 | 2015-03-25 | 河北大学 | Method for cultivating auricularia polytricha through thorn scraps |
| CN104478547A (en) * | 2014-11-03 | 2015-04-01 | 河北大学 | Method for utilizing vitex negundo var ineica scrap to culture pholiota adiposa |
| CN105191666A (en) * | 2015-09-24 | 2015-12-30 | 榕江县苗岭菌类种植加工厂 | Method for imitating wild cultivation of ganoderma atrum |
| CN106576896A (en) * | 2016-11-22 | 2017-04-26 | 曹晓龙 | Method for preparing stock seeds of high-mountain ganoderma lucidum |
| CN106665124A (en) * | 2017-01-18 | 2017-05-17 | 贵州省山地资源研究所有限公司 | Cultivation method for returning coriaria sinia mushroom to nature |
| CN109006163A (en) * | 2018-07-03 | 2018-12-18 | 沈阳农业大学 | A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method |
| CN108617410A (en) * | 2018-08-13 | 2018-10-09 | 轩先森 | A kind of culture medium of edible fungus material and the preparation method and application thereof made by hazel material |
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