CN109006163A - A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method - Google Patents
A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method Download PDFInfo
- Publication number
- CN109006163A CN109006163A CN201810714956.6A CN201810714956A CN109006163A CN 109006163 A CN109006163 A CN 109006163A CN 201810714956 A CN201810714956 A CN 201810714956A CN 109006163 A CN109006163 A CN 109006163A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- ganoderma
- cultivar
- bacterium bag
- ganoderma tsugae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to ganoderma lucidum artificial cultivation technique fields, more particularly to a kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method, by mass percentage, culture medium includes sawdust 25-30%, cotton seed hull 35-40%, wheat bran 15-20%, corncob 8-12%, maize flour 2-5%, gypsum 1-3%, calcium superphosphate 0.5-1.5%, lime 0.5-1.5%, above-mentioned each ingredient total moisture content 60-70%, and adds 0.5-0.7g sodium selenite in every kg broth.The present invention can make Ganoderma tsugae fructification quality close, and high-quality, sesame is neat out, make Ganoderma tsugae increase harvest.
Description
Technical field
The invention belongs to ganoderma lucidum artificial cultivation technique fields, and in particular to a kind of Ganoderma tsugae cultivar cultural method, training
Support base and culture medium preparation method.
Background technique
Ganoderma tsugae also known as ganoderma tsugae, Chinese hemlock spruce tree sesame, Ganoderma Lucidum belong to Ganodermataceae Ganoderma, are China Northeast Forest Areas spies
Some lucidum varieties are mainly grown on 700~1400m of height above sea level Korean pine and broad-leaved mixed forests, the fir in needle mixed forest, fallen leaves
Pine, the stump of dragon spruce, in rotten wood, is a kind of extremely rare precious herbal medicine, is mainly distributed on China's cool temperature zone and (originates in Korean pine
Changbaishan area) area.Fructification is annual, and suberin has handle to wooden.Cap kidney shape, semicircle or close fan-shaped, capping is red
Brown has paint shape gloss, lid edge is thin or blunt, sometimes involute to aubergine.Bacterial context pale, white to canescence, mycelia without
Color is to filbert.Succulence when bacterial context is fresh, it is light after doing.Stem is thick and short, surface atropurpureus, has paint shape gloss.Stem
It is unclear with the boundary line of cap, normal horizontal obtuse angle.Spore is oval, and top is often truncate.Current wild Ganoderma tsugae resource quantity
It is limited and fewer and fewer.Studies have shown that Ganoderma tsugae rich in nutrition content, contain amino acid needed by human, nucleosides, micro-
Secondary element, vitamin and bioactive polysaccharide, triterpene compound, alkaloid etc., while still maintenance human health institute is necessary
Dietary fiber good source.Medical research proves that Ganoderma tsugae is removed to hepatitis, arthritis, diabetes, hypertension, high blood
The diseases such as rouge, neurasthenia are effectively outer, also have significant curative effect to the big lethal disease coronary heart disease of the mankind three, cancer, cerebral hemorrhage；Tool
Have it is antitumor, improve immunity of organisms, it is antiviral, remove free radical, anti-aging, antithrombotic, anticoagulation the effects of；Ganoderma tsugae
In alkaloid have improve coronary blood flow, reduce myocardial oxygen consumption, enhancing cardiac muscle and body anti-anoxia ability and
The diseases such as coronary heart disease, hypertension, hyperlipemia, hepatitis, myasthenia are had significant curative effect by the effect for reducing cholesterol；Song Shanling
Gradient elution adenosine, uridine, adenine, uracil isoreactivity ingredient in sesame are that Ganoderma tsugae has mutation-resisting functional
Material base；Contain 18 kinds of amino acid in Ganoderma tsugae fructification, wherein essential amino acid content is compared with other edible mushrooms height
The average content of the lower methionine of content in 40%, especially edible mushroom, Ganoderma tsugae is much higher than other edible mushrooms.Study table
Bright Ganoderma tsugae fructification contains the various trace elements such as Mn, Cr, Cu, Fe, Ca, Mg, Zn, Se, Ge, and Ganoderma tsugae also contains greatly
The vitamin of amount, wherein the content of VitC, VitE and carrotene is higher, has significant anti-oxidant, activity of fighting against senium.
Currently, China's ganoderma lucidum industry mainly based on planting industry, is main ganoderma lucidum producting and exporting country in the world.But
Based on peasant household's cultivation, scale, standardized plantation are less, and new plantation, cultivation technique research and development strength be not strong, mostly uses with original
Wood is the planting patterns of raw material, and the more forest reserves are lost.Meanwhile the planting patterns based on peasant household, ganoderma lucidum quality are difficult
With control, cause part ganoderma lucidum second-rate, agriculture is residual and heavy metals exceeding standard, and the ganoderma lucidum source of goods of high-quality is insufficient, it is difficult to for processing
Product provides quality raw materials.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of Ganoderma tsugae cultivar cultural method, culture medium and trainings
Base preparation method is supported, Ganoderma tsugae is planted for standardization artificial cultivation and guidance is provided.
The invention is realized in this way on the one hand a kind of Ganoderma tsugae Cultivar culture medium is provided, and by mass percentage, packet
Include sawdust 25-30%, cotton seed hull 35-40%, wheat bran 15-20%, corncob 8-12%, maize flour 2-5%, gypsum 1-3%, mistake
Calcium phosphate 0.5-1.5%, lime 0.5-1.5%, above-mentioned each ingredient total moisture content 60-70%, and add in every kg broth
0.5-0.7g sodium selenite.
Including sawdust 28%, cotton seed hull 37%, wheat bran 18%, corncob 10%, maize flour further, in mass ratio,
3%, gypsum 2%, calcium superphosphate 1%, lime 1%, above-mentioned each ingredient total moisture content 65%, and add in every kg broth
0.6g sodium selenite.
The present invention also provides a kind of preparation methods of Ganoderma tsugae Cultivar culture medium, include the following steps:
1) using the log or sawdust of one or more pine trees in abies holophylla, black pine, larch, 95-105 is heated up to after crushing
DEG C boiling 8-10 hours, sunning allowed particles of pine to give full play to grease and antibacterial substance, and it is standby that raft base-material is collected together into after drying completely
With；
2) each ingredient of culture medium is weighed according to the formula as described in any of the above-described, is mixed；
3) culture medium in step 2) is sterilized in high-pressure steam sterilizing pan, pressure 0.15MPa, time 1.5-2h,
It is down to 30 DEG C or less；
4) culture medium of the 1.2kg to sterilize in the 5kg raft base-material and step 3) that prepare in step 1) is packed into polybag
In, it is uniformly mixed, obtains Ganoderma tsugae Cultivar culture medium.
The present invention also provides a kind of cultural methods of Ganoderma tsugae cultivar, include the following steps:
A) Ganoderma tsugae Cultivar culture medium is prepared according to above-mentioned method；
B) in sterile board, toward culture medium on be inoculated with lucid ganoderma stock culture, inoculated polybag is placed on by sterilizing, divulging information
In dry culturing room, 23-27 DEG C of temperature, culture 40-50 days, until mycelia covers with polybag；
C) after Duan Muyong aerosol disinfection box fumigation 25-35min being inoculated with, bundle, the plastics in opening steps b) are bundled into
Bag ties section wood after the culture medium for covering with mycelia in polybag to be sprinkled into the surface layer and gap of section wood bundle near Alcohol Flame
Tying immediately is put into polybag；
D) polybag equipped with the section wood bundle for connecting kind is placed in culturing room, temperature is 26-30 DEG C, humidity 40-
65%, ventilation 6-8 hour, culture are loosened tying rope and are continued to cultivate to 45-55 days daily after 15-20 days, mycelia purseful, followed by
Continuous culture 15-20 days, until mycelia physiological maturity, forms bacterium bag, can descend ground.
Further, bacterium bag cultured in step d) progress hayashishita is imitated wild corresponding cultivation: late May is raw by mycelia
The mature bacterium bag of reason is placed in standing forest, is in the ranks put along slope as wide 80-100cm, the double-deck bacterium bag of long 30-50m, between bacterium bag
Away from for 5-10cm, the mixture 1cm of upper earthing and biomass, keeping furrow face wet, plastic film canopy is set on furrow；It cultivates to young sesame
It breaks ground after showing up, water management follows alternation of wetting and drying, i.e. the both ends moisturizing of night closing plastic film canopy, daytime opens；Plastics
Micro- spray is disposed in film canopy, respectively sprays 10-20min in the morning, afternoon and evening, when the cap edge of ganoderma lucidum fruitbody becomes bronzing, reduces spray
Water；
When white growth circle in cap edge starts to disappear, color also starts from light to dark, and cap no longer increases, and spore is continuous
When distributing, ganoderma lucidum fruitbody is harvested.
Further, bacterium bag cultured in step d) is carried out hayashishita stump pseudo-wild cultivating: late May is by diameter
The pine of 20-30cm is cut into the juggle of 50cm long, and the hole for boring a length 300mm on juggle with the electric drill of diameter 110cm is standby
With, by bacterium bag and pine section be transported to cultivation woods in, on the ground dig diameter 130cm, the hole of deep 40cm, every hole fills 200 or so bacterium bags
And remove bacterium bag crust, then pine section is stood in bacterium bag hole, bacterium bag is also placed in the hole of juggle, then earthing 20cm,
Pine section exposes soil face 30cm, and bacterium bag is all buried sternly, spacing >=1m of each cave ganoderma lucidum, and late growth temperature is controlled in 26-28
DEG C, it keeps relative air humidity in 80-90% or so, divulges information 8-10 hours daily；
When white growth circle in cap edge starts to disappear, color also starts from light to dark, and cap no longer increases, and spore is continuous
When distributing, ganoderma lucidum fruitbody is harvested.
Compared with the prior art, the advantages of the present invention are as follows:
A kind of culture medium and cultural method for cultivating loose China fir Linzhi's cultivar is provided, keeps Ganoderma tsugae fructification quality tight
Close, high-quality, sesame is neat out, makes Ganoderma tsugae increase harvest.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below with reference to embodiment, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein is used only for explaining the present invention, it is not used to
Limit the present invention.
4 kinds of culture medium batching modes are designed as, investigation and comparison, which filters out, is suitable for the optimal cultivar culture of Ganoderma tsugae
The batching mode of base.A: cotton seed hull 88%, wheat bran 10%, sugar 1%, gypsum 1%, water content 65%；B: sawdust 77%, wheat bran
18%, maize flour 3%, sugar 1%, gypsum 1%, water content 65%；C: corncob 40%, sawdust 45%, wheat bran 10%, corn
Face 4%, gypsum 1%, water content 65%；D: sawdust 28%, cotton seed hull 37%, wheat bran 18%, corncob 10%, maize flour 3%,
Gypsum 2%, calcium superphosphate 1%, lime 1%, water content 65%.
Influence of 4 kinds of different Cultivar culture mediums to Ganoderma tsugae fructification is as shown in table 1, as it can be seen from table 1 participating in the experiment
4 kinds of Cultivar culture mediums in, Ganoderma tsugae bacterial strain yield highest in A proportion Cultivar culture medium, total output reaches
5050g, but fructification quality is loose；The yield column second of Ganoderma tsugae bacterial strain, total output reach on D proportion culture medium
4400g, but fructification quality is close, it is high-quality.Thus, synthesis of the Ganoderma tsugae bacterial strain in D proportion Cultivar culture medium is commented
Valence highest, biological efficiency column second, up to 10.5%, but fructification quality is close, high-quality, this illustrates that cotton seed skin can improve
The yield of Ganoderma tsugae fructification, in ingredient plus a certain amount of sawdust helps to improve the quality of fructification.
Table 1
Embodiment 1,
The cultural method of Ganoderma tsugae cultivar, includes the following steps:
1) using the log or sawdust of one or more pine trees in abies holophylla, black pine, larch, 100 DEG C are heated up to after crushing
Boiling 9 hours, sunning allowed particles of pine to give full play to grease and antibacterial substance, and it is spare that raft base-material is collected together into after drying completely；
2) each ingredient of culture medium is weighed according to following formulas, is mixed: by mass percentage, including sawdust 28%,
Cotton seed hull 37%, wheat bran 18%, corncob 10%, maize flour 3%, gypsum 2%, calcium superphosphate 1%, lime 1%, it is above-mentioned each
Ingredient total moisture content 65%, and add 0.6g sodium selenite in every kg broth.
3) culture medium in step 2) is sterilized in high-pressure steam sterilizing pan, pressure 0.15MPa, time 1.7h, drop
To 30 DEG C or less；
4) culture medium of the 1.2kg to sterilize in the 5kg raft base-material and step 3) that prepare in step 1) is packed into polybag
In, it is uniformly mixed, obtains Ganoderma tsugae Cultivar culture medium.
5) in sterile board, toward culture medium on be inoculated with lucid ganoderma stock culture, inoculated polybag is placed on by sterilizing, divulging information
In dry culturing room, 25 DEG C of temperature, culture 45 days, until mycelia covers with polybag；
6) will inoculation Duan Muyong aerosol disinfection box fumigation 30min after, be bundled into bundle, opening steps 5) in polybag,
After the culture medium for covering with mycelia in polybag to be sprinkled into the surface layer and gap of section wood bundle near Alcohol Flame, section wood bundle is put into
Tying immediately in polybag；
7) polybag equipped with the section wood bundle for connecting kind is placed in culturing room, temperature is 28 DEG C, and humidity 42% is logical daily
Wind 7h, culture loosen tying rope and continue culture to 50 days after 17 days, mycelia purseful is cultivated for 17 days, until mycelia physiology
Maturation forms bacterium bag, can descend ground.
Bacterium bag cultured in step 7) progress hayashishita is imitated wild corresponding cultivation: late May is by mycelia physiological maturity
Bacterium bag is placed in standing forest, is in the ranks put along slope as wide 90cm, the double-deck bacterium bag of long 40m, and bacterium bag spacing is 7cm, upper earthing and
The mixture 1cm of biomass keeps furrow face wet, plastic film canopy is set on furrow；After culture breaks ground to young sesame and shows up, water management
Alternation of wetting and drying is followed, i.e. the both ends moisturizing of night closing plastic film canopy, daytime opens；Micro- spray is disposed in plastic film canopy, it is early
Middle evening each spray 15min, when the cap edge of ganoderma lucidum fruitbody becomes bronzing, reduces water spray；
When white growth circle in cap edge starts to disappear, color also starts from light to dark, and cap no longer increases, and spore is continuous
When distributing, ganoderma lucidum fruitbody is harvested.
Embodiment 2,
The cultural method of Ganoderma tsugae cultivar, includes the following steps:
1) using the log or sawdust of one or more pine trees in abies holophylla, black pine, larch, 95 DEG C of steamings are heated up to after crushing
It boils 8 hours, sunning allows particles of pine to give full play to grease and antibacterial substance, and it is spare that raft base-material is collected together into after drying completely；
2) each ingredient of culture medium is weighed according to following formulas, is mixed: by mass percentage, including sawdust 25%,
Cotton seed hull 35%, wheat bran 15%, corncob 8%, maize flour 2%, gypsum 1%, calcium superphosphate 0.5%, lime 0.5%, it is above-mentioned
Each ingredient total moisture content 60%, and add 0.5g sodium selenite in every kg broth.
3) culture medium in step 2) is sterilized in high-pressure steam sterilizing pan, pressure 0.15MPa, time 1.5h, drop
To 30 DEG C or less；
4) culture medium of the 1.2kg to sterilize in the 5kg raft base-material and step 3) that prepare in step 1) is packed into polybag
In, it is uniformly mixed, obtains Ganoderma tsugae Cultivar culture medium.
5) in sterile board, toward culture medium on be inoculated with lucid ganoderma stock culture, inoculated polybag is placed on by sterilizing, divulging information
In dry culturing room, 23 DEG C of temperature, culture 40 days, until mycelia covers with polybag；
6) will inoculation Duan Muyong aerosol disinfection box fumigation 25min after, be bundled into bundle, opening steps 5) in polybag,
After the culture medium for covering with mycelia in polybag to be sprinkled into the surface layer and gap of section wood bundle near Alcohol Flame, section wood bundle is put into
Tying immediately in polybag；
7) polybag equipped with the section wood bundle for connecting kind is placed in culturing room, temperature is 26 DEG C, and humidity 40% is logical daily
Wind 6h, culture loosen tying rope and continue culture to 45 days after 15 days, mycelia purseful is cultivated for 15 days, until mycelia physiology
Maturation forms bacterium bag, can descend ground.
Bacterium bag cultured in step 7) is carried out hayashishita stump pseudo-wild cultivating: late May is by the pine of diameter 20cm
It is cut into the juggle of 50cm long, the hole for boring a length 300mm on juggle with the electric drill of diameter 110cm is spare, by bacterium bag and pine
Juggle is transported in cultivation woods, digs diameter 130cm on the ground, the hole of deep 40cm, every hole fills 200 or so bacterium bags and shells bacterium bag crust
From, then pine section is stood in bacterium bag hole, bacterium bag is placed into the hole of juggle, then earthing 20cm, the native face of pine section exposing
30cm, bacterium bag are all buried sternly, spacing >=1m of each cave ganoderma lucidum, and late growth temperature is controlled at 26 DEG C, keep air relatively wet
Degree is divulged information 8 hours daily 80% or so；
When white growth circle in cap edge starts to disappear, color also starts from light to dark, and cap no longer increases, and spore is continuous
When distributing, ganoderma lucidum fruitbody is harvested.
Embodiment 3,
The cultural method of Ganoderma tsugae cultivar, includes the following steps:
1) using the log or sawdust of one or more pine trees in abies holophylla, black pine, larch, 105 DEG C are heated up to after crushing
Boiling 10 hours, sunning allowed particles of pine to give full play to grease and antibacterial substance, and it is spare that raft base-material is collected together into after drying completely；
2) each ingredient of culture medium is weighed according to following formulas, is mixed: by mass percentage, including sawdust 30%,
Cotton seed hull 40%, wheat bran 20%, corncob 12%, maize flour 5%, gypsum 3%, calcium superphosphate 1.5%, lime 1.5%, it is above-mentioned
Each ingredient total moisture content 70%, and add 0.7g sodium selenite in every kg broth.
3) culture medium in step 2) is sterilized in high-pressure steam sterilizing pan, pressure 0.15MPa, time 2h are down to
30 DEG C or less；
4) culture medium of the 1.2kg to sterilize in the 5kg raft base-material and step 3) that prepare in step 1) is packed into polybag
In, it is uniformly mixed, obtains Ganoderma tsugae Cultivar culture medium.
5) in sterile board, toward culture medium on be inoculated with lucid ganoderma stock culture, inoculated polybag is placed on by sterilizing, divulging information
In dry culturing room, 27 DEG C of temperature, culture 50 days, until mycelia covers with polybag；
6) will inoculation Duan Muyong aerosol disinfection box fumigation 35min after, be bundled into bundle, opening steps 5) in polybag,
After the culture medium for covering with mycelia in polybag to be sprinkled into the surface layer and gap of section wood bundle near Alcohol Flame, section wood bundle is put into
Tying immediately in polybag；
7) polybag equipped with the section wood bundle for connecting kind is placed in culturing room, temperature is 30 DEG C, and humidity 65% is logical daily
Wind 8h, culture loosen tying rope and continue culture to 55 days after 20 days, mycelia purseful is cultivated for 20 days, until mycelia physiology
Maturation forms bacterium bag, can descend ground.
Bacterium bag cultured in step 7) progress hayashishita is imitated wild corresponding cultivation: late May is by mycelia physiological maturity
Bacterium bag is placed in standing forest, is in the ranks put along slope as wide 100cm, the double-deck bacterium bag of long 50m, and bacterium bag spacing is 10cm, upper earthing
With the mixture 1cm of biomass, keeps furrow face wet, plastic film canopy is set on furrow；After culture breaks ground to young sesame and shows up, moisture pipe
Reason follows alternation of wetting and drying, i.e. the both ends moisturizing of night closing plastic film canopy, daytime opens；Micro- spray is disposed in plastic film canopy,
20min is respectively sprayed in the morning, afternoon and evening, when the cap edge of ganoderma lucidum fruitbody becomes bronzing, reduces water spray；
When white growth circle in cap edge starts to disappear, color also starts from light to dark, and cap no longer increases, and spore is continuous
When distributing, ganoderma lucidum fruitbody is harvested.
Ganoderma lucidum obtained in embodiment 1,2,3 is detected, it is found that sodium selenite can be improved ganoderma lucidum fruitbody
Yield increases that Se content and fruiting in ganoderma lucidum body are neat, improve ganoderma lucidum vivo protein, polysaccharide, amino acid content.
Claims (6)
1. a kind of Ganoderma tsugae Cultivar culture medium, which is characterized in that by mass percentage, including sawdust 25-30%, cotton seed hull
35-40%, wheat bran 15-20%, corncob 8-12%, maize flour 2-5%, gypsum 1-3%, calcium superphosphate 0.5-1.5%, lime
0.5-1.5%, above-mentioned each ingredient total moisture content 60-70%, and add 0.5-0.7g sodium selenite in every kg broth.
2. Ganoderma tsugae Cultivar culture medium as described in claim 1, which is characterized in that in mass ratio, including sawdust 28%,
Cotton seed hull 37%, wheat bran 18%, corncob 10%, maize flour 3%, gypsum 2%, calcium superphosphate 1%, lime 1%, it is above-mentioned each
Ingredient total moisture content 65%, and add 0.6g sodium selenite in every kg broth.
3. a kind of preparation method of Ganoderma tsugae Cultivar culture medium, which comprises the steps of:
1) using the log or sawdust of one or more pine trees in abies holophylla, black pine, larch, 95-105 DEG C of steaming is heated up to after crushing
It boils 8-10 hours, sunning allows particles of pine to give full play to grease and antibacterial substance, and it is spare that raft base-material is collected together into after drying completely；
2) each ingredient that culture medium is weighed according to formula as described in claim 1 or 2 is any, mixes；
3) culture medium in step 2) is sterilized in high-pressure steam sterilizing pan, pressure 0.15MPa, time 1.5-2h are down to
30 DEG C or less；
4) culture medium of the 1.2kg to sterilize in the 5kg raft base-material prepared in step 1) and step 3) is fitted into polybag, is mixed
It closes uniformly, obtains Ganoderma tsugae Cultivar culture medium.
4. a kind of cultural method of Ganoderma tsugae cultivar, which comprises the steps of:
A) Ganoderma tsugae Cultivar culture medium is prepared according to the method for claim 3；
B) in sterile board, toward culture medium on be inoculated with lucid ganoderma stock culture, by inoculated polybag be placed on by sterilizing, aeration-drying
Culturing room in, 23-27 DEG C of temperature, culture 40-50 days, until mycelia covers with polybag；
C) bundle will be bundled into after inoculation Duan Muyong aerosol disinfection box fumigation 25-35min, the polybag in opening steps b),
After the culture medium for covering with mycelia in polybag is nearby sprinkled into the surface layer and gap of section wood bundle by Alcohol Flame, section wood bundle is put into modeling
Tying immediately in material bag；
D) polybag equipped with the section wood bundle for connecting kind is placed in culturing room, temperature is 26-30 DEG C, humidity 40-65%, daily
Ventilation 6-8 hours, culture loosen tying rope and continue culture to 45-55 days after 15-20 days, mycelia purseful is cultivated for 15-
20 days, until mycelia physiological maturity, forms bacterium bag, can descend ground.
5. the cultural method of Ganoderma tsugae cultivar as claimed in claim 4, which is characterized in that will be cultured in step d)
Bacterium bag carries out hayashishita and imitates wild corresponding cultivation: the bacterium bag of mycelia physiological maturity is placed in standing forest by late May, is in the ranks put along slope
It puts as wide 80-100cm, the double-deck bacterium bag of long 30-50m, bacterium bag spacing is 5-10cm, the mixture 1cm of upper earthing and biomass,
It keeps furrow face wet, plastic film canopy is set on furrow；After culture breaks ground to young sesame and shows up, water management follows alternation of wetting and drying, i.e. night
Between close plastic film canopy both ends moisturizing, daytime open；Micro- spray is disposed in plastic film canopy, respectively sprays 10-20min in the morning, afternoon and evening,
When the cap edge of ganoderma lucidum fruitbody becomes bronzing, water spray is reduced；
When white growth circle in cap edge starts to disappear, color also starts from light to dark, and cap no longer increases, and spore constantly distributes
When, harvest ganoderma lucidum fruitbody.
6. the cultural method of Ganoderma tsugae cultivar as claimed in claim 4, which is characterized in that will be cultured in step d)
Bacterium bag carries out hayashishita stump pseudo-wild cultivating: the pine of diameter 20-30cm is cut into the juggle of 50cm long by late May, uses diameter
The hole that the electric drill of 110cm bores a length 300mm on juggle is spare, and bacterium bag and pine section are transported in cultivation woods, dug on the ground
Diameter 130cm, the hole of deep 40cm, every hole fill 200 or so bacterium bags and remove bacterium bag crust, and pine section is then stood on bacterium bag
In hole, bacterium bag is also placed in the hole of juggle, then earthing 20cm, pine section exposes soil face 30cm, and bacterium bag is all buried sternly, each cave
Spacing >=1m of ganoderma lucidum, late growth temperature are controlled at 26-28 DEG C, are kept relative air humidity in 80-90% or so, are led to daily
Wind 8-10 hours；
When white growth circle in cap edge starts to disappear, color also starts from light to dark, and cap no longer increases, and spore constantly distributes
When, harvest ganoderma lucidum fruitbody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810714956.6A CN109006163A (en) | 2018-07-03 | 2018-07-03 | A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810714956.6A CN109006163A (en) | 2018-07-03 | 2018-07-03 | A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109006163A true CN109006163A (en) | 2018-12-18 |
Family
ID=65521418
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810714956.6A Pending CN109006163A (en) | 2018-07-03 | 2018-07-03 | A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109006163A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110115196A (en) * | 2019-05-22 | 2019-08-13 | 吉林大学 | A kind of ganoderma lucidum cultivation method |
| CN112166950A (en) * | 2020-10-13 | 2021-01-05 | 大兴安岭地区农业林业科学研究院 | Near-natural cultivation method and cultivation device for ganoderma tsugae |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631078A (en) * | 2003-12-23 | 2005-06-29 | 马士贵 | Wild-imitating mushroom edible fungus seed and cultivation method thereof |
| CN101288374A (en) * | 2008-06-08 | 2008-10-22 | 李朝谦 | Breeding method of selenium-enriched log glossy ganoderma spore powder |
| CN102870595A (en) * | 2012-09-13 | 2013-01-16 | 广东省微生物研究所 | Artificial cultivation method capable of realizing highland cultivation of wild ganoderma tsugae murr |
| CN104041330A (en) * | 2014-07-09 | 2014-09-17 | 通化吉通实业有限公司 | Ganoderma tsugae imitating wild short-cut wood cultivation method |
| CN104255294A (en) * | 2014-09-15 | 2015-01-07 | 董学堂 | Physical-combination planting process of living grafted lucid ganoderma ornamental bonsai |
| CN104798599A (en) * | 2015-04-22 | 2015-07-29 | 吉林农业大学 | Method for planting ganoderma tsugae by imitating wild growth in forest land |
| CN105493883A (en) * | 2014-09-25 | 2016-04-20 | 申茂军 | Ganoderma tsugae cultivation technology |
| CN105706732A (en) * | 2016-01-27 | 2016-06-29 | 泰安市农业科学研究院 | Method for cultivating ganoderma applanatum |
| CN105940960A (en) * | 2016-06-15 | 2016-09-21 | 句容市方山茶场 | Soil-covered cultivation method of interplanting lucid ganoderma in tea garden |
| CN106305135A (en) * | 2016-08-17 | 2017-01-11 | 吴庆旺 | Coriolus and lucid ganoderma edible mushroom and preparation method thereof |
| CN106613324A (en) * | 2016-11-16 | 2017-05-10 | 广西壮族自治区林业科学研究院 | Ganoderma undergrowth bionics wild cultivation method |
-
2018
- 2018-07-03 CN CN201810714956.6A patent/CN109006163A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631078A (en) * | 2003-12-23 | 2005-06-29 | 马士贵 | Wild-imitating mushroom edible fungus seed and cultivation method thereof |
| CN101288374A (en) * | 2008-06-08 | 2008-10-22 | 李朝谦 | Breeding method of selenium-enriched log glossy ganoderma spore powder |
| CN102870595A (en) * | 2012-09-13 | 2013-01-16 | 广东省微生物研究所 | Artificial cultivation method capable of realizing highland cultivation of wild ganoderma tsugae murr |
| CN104041330A (en) * | 2014-07-09 | 2014-09-17 | 通化吉通实业有限公司 | Ganoderma tsugae imitating wild short-cut wood cultivation method |
| CN104255294A (en) * | 2014-09-15 | 2015-01-07 | 董学堂 | Physical-combination planting process of living grafted lucid ganoderma ornamental bonsai |
| CN105493883A (en) * | 2014-09-25 | 2016-04-20 | 申茂军 | Ganoderma tsugae cultivation technology |
| CN104798599A (en) * | 2015-04-22 | 2015-07-29 | 吉林农业大学 | Method for planting ganoderma tsugae by imitating wild growth in forest land |
| CN105706732A (en) * | 2016-01-27 | 2016-06-29 | 泰安市农业科学研究院 | Method for cultivating ganoderma applanatum |
| CN105940960A (en) * | 2016-06-15 | 2016-09-21 | 句容市方山茶场 | Soil-covered cultivation method of interplanting lucid ganoderma in tea garden |
| CN106305135A (en) * | 2016-08-17 | 2017-01-11 | 吴庆旺 | Coriolus and lucid ganoderma edible mushroom and preparation method thereof |
| CN106613324A (en) * | 2016-11-16 | 2017-05-10 | 广西壮族自治区林业科学研究院 | Ganoderma undergrowth bionics wild cultivation method |
Non-Patent Citations (2)
| Title |
|---|
| 李福强等: "松杉灵芝仿野生栽培技术", 《吉林林业科技》 * |
| 陈若霞等: "小直径果木枝条仿段木栽培灵芝关键技术", 《食用菌》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110115196A (en) * | 2019-05-22 | 2019-08-13 | 吉林大学 | A kind of ganoderma lucidum cultivation method |
| CN112166950A (en) * | 2020-10-13 | 2021-01-05 | 大兴安岭地区农业林业科学研究院 | Near-natural cultivation method and cultivation device for ganoderma tsugae |
| CN112166950B (en) * | 2020-10-13 | 2022-07-19 | 大兴安岭地区农业林业科学研究院 | Near-natural cultivation method and cultivation device for ganoderma tsugae |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104041330B (en) | Ganoderma tsugae imitates wild juggle cultivation method | |
| CN105940960B (en) | A kind of soil covering culture method of interplanting ganoderma lucidum in tea gardens | |
| CN101707966B (en) | Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same | |
| CN103864491B (en) | A kind of Ganoderma tsugae culture material and cultivating method thereof being suitable for the Northeast's cultivation | |
| CN105900690A (en) | Pleurotus ostreatus cultivation method | |
| CN104798601A (en) | Cultivation method for lentinula edodes | |
| CN102150615A (en) | Method for culturing, planting and mycorrhizal production of dendrobium officinale kimura et migo | |
| CN106358755A (en) | Interplanting cultivation method of lentinula edodes, morchella vulgarises and kudzuvine roots | |
| CN105900686A (en) | Tremella substitute cultivation method | |
| KR101383550B1 (en) | Growth-promoting rice using effective micro-organisms and cultivation method thereof | |
| CN106888894A (en) | A kind of implantation methods of selenium-rich pawpaw | |
| CN107360858A (en) | A kind of breeding method of mushroom edible mushroom | |
| CN104012303A (en) | Cultivating method of pleurotus geesteranus edible mushroom | |
| CN103444433B (en) | Artificial cultivation method for Trametes cinnabarina | |
| CN109006163A (en) | A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method | |
| CN1557122A (en) | Schizophyllum commune artificial cultivation and breeding technology | |
| CN107125017A (en) | A kind of breeding method of mushroom | |
| CN105110852A (en) | Liquid strain culture medium for shiitake mushrooms and method for culturing shiitake mushrooms in three-dimensional bag hanging mode | |
| CN106748362A (en) | A kind of method for planting dendrobium candidum in the tree | |
| CN106688622A (en) | Culture medium formula of artificially cultivated armillaria mellea and cultivation method | |
| KR100723068B1 (en) | Method for culturing flammulina velutipes including ginseng saponin | |
| CN107736203A (en) | A kind of as if field winter bamboo shoot implantation methods | |
| CN106941960A (en) | A kind of cultivation matrix of roxburgh anoectochilus terminal bud | |
| CN105766578A (en) | High-quality imitating-wild agaric chaff dendrobium nobile planting method | |
| CN105052543B (en) | A kind of method of artificial cultivation Chinese wax umbrella |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181218 |
|
| RJ01 | Rejection of invention patent application after publication |