CN105706732A - Method for cultivating ganoderma applanatum - Google Patents
Method for cultivating ganoderma applanatum Download PDFInfo
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- CN105706732A CN105706732A CN201610056328.4A CN201610056328A CN105706732A CN 105706732 A CN105706732 A CN 105706732A CN 201610056328 A CN201610056328 A CN 201610056328A CN 105706732 A CN105706732 A CN 105706732A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
Abstract
The invention belongs to the field of habituated culture of rare wild medicinal fungi and particularly relates to a method for cultivating ganoderma applanatum.The method comprises the steps of fungus type selection, breeder seed and cultivation seed preparation, mycelium culture and ganoderma applanatum cultivation.According to the method, a fruiting strain is obtained out of ganoderma applanatum sporocarp which is obtained from the wild through a tissue isolation method, then the fruiting strain is cultivated into original mother seeds which are high in stability, great in metabolic capability and high in safety through system breeding, and plantation is conducted by preparing the mother seeds, original seeds and cultivation seeds and controlling the environmental condition, so that the ganoderma applanatum sporocarp is obtained.By means of the cultivation method, the cultivated ganoderma applanatum is high in output and high in polysaccharide content, and the method is low in cost and easy to operate and is a ganoderma applanatum cultivation method which is of high development and promotional value.
Description
Technical field
The invention belongs to the domestication of rare wild medical fungi to cultivate, more specifically to the cultural method of a kind of Ganoderma applanatum (Pers.) Pat.
Background technology
Ganoderma applanatum (Pers.) Pat (Ganodermaapplanatum) is under the jurisdiction of Basidiomycotina (Basidiomycotina), Hymenomycetes (Hymenomycetes), Aphyllophorales (Aphyllophorales), Ganoderma Cordycepps (Ganodermataceae), Ganoderma (Ganoderma), for the medicinal fungi that China is traditional, Ganoderma applanatum (Pers.) Pat is put down, bitter in the mouth, having the multiple efficacies such as adjustment immunity of organisms, antitumor, inhibiting bacteria and diminishing inflammation, clinic is used for treatment hepatitis B, esophageal carcinoma, pulmonary tuberculosis, neurasthenia etc..Along with constantly finding and its health-care effect prominent of the new medicinal efficacy of Ganoderma applanatum (Pers.) Pat, market demand significantly increases, and has a extensive future.
In recent years due to the destruction of ecological environment with excessively excavate, Ganoderma applanatum (Pers.) Pat wild resource is fewer and feweri.The research of Ganoderma applanatum (Pers.) Pat has focused largely on the aspects such as separation and the pharmacology activity research of extract both at home and abroad at present, wherein Ganoderma Applanatum Polysaccharides is always up the focus of research, the polysaccharide and other multiple active components that Ganoderma applanatum (Pers.) Pat contains can suppress hepatitis B virus, regulate body's immunity, suppress platelet aggregation, develop the medicine into treating hepatitis B, such as " compound recipe Ganoderma applanatum (Pers. Ex Wallr) Pat. sheet ", " liver must be answered " and " Ganoderma applanatum (Pers. Ex Wallr) Pat. liver Thailand " etc..Ganoderma applanatum (Pers.) Pat domestication culture studies is less, has a strong impact on the exploitation of Ganoderma applanatum (Pers.) Pat wild resource.In addition rapidly going up of cotton seed hulls price causes that cultivation cost increases, and is therefore badly in need of the new Ganoderma applanatum (Pers.) Pat bacterial strain of cultivation, NEW TYPE OF COMPOSITE cultivation formula and breeding method.
Summary of the invention
The technical problem to be solved in the present invention is to provide the cultural method of a kind of new varieties Ganoderma applanatum (Pers.) Pat, it is achieved the generation material artificial culture of Ganoderma applanatum (Pers.) Pat, solves the Sustainable Use Problems of Ganoderma applanatum (Pers.) Pat natural resources.
For solving above-mentioned technical problem, the present invention provides the cultural method of a kind of Ganoderma applanatum (Pers.) Pat, described method to include strain selection, female preparation, original seed and the preparation of cultigen, mycelia culture, Ganoderma applanatum (Pers.) Pat cultivation planted.
(1) strain selects:
Choose wild Ganoderma applanatum (Pers.) Pat as kind of a source, extract the parent in wild Ganoderma applanatum (Pers.) Pat, through the acclimatization culture to parent, by the Ganoderma applanatum (Pers.) Pat strain of the former parent acclimatization culture Cheng Xin of wild Ganoderma applanatum (Pers.) Pat.
(2) female preparation planted:
Preparation expands mother's kind, adds PDA culture medium, access the Ganoderma applanatum (Pers.) Pat strain in above-mentioned (1) after sterilizing cooling in test tube, and cultivation temperature is 25 DEG C～30 DEG C, cultivates 7-10 days, and mycelia covers with media surface becomes ripe female kind.
(3) preparation of original seed:
Selecting materials and get the raw materials ready, choosing cotton seed hulls, corn cob and weed tree sawdust is cultivation matrix, dries standby；
Prepared by original seed, the pedigree seed culture medium configured is loaded in original seeds bottle, autoclaving 1.5kg/cm2 keeps 3h, it is cooled to less than 30 DEG C female kind ripe in above-mentioned (2) aseptically inoculated, being placed under 20 DEG C～25 DEG C conditions lucifuge and cultivate, mycelia covers with original seeds bottle becomes ripe original seed.
(4) preparation of cultigen:
Cultivar culture medium not sugaring; the same pedigree seed culture medium of all the other proportionings; cultivating bag adopts 17cm × 39cm polypropylene plastics pocket; atmospheric steam sterilizing is carried out in time after pack; 12h is maintained when temperature reaches 100 DEG C; after truce, stewing 12h can take the dish out of the pot the bag that dries in the air again, treats that pocket temperature is down to less than 28 DEG C and is aseptically inoculated by maturation original seed in above-mentioned (3), and cultigen condition of culture is identical with Primary spawn condition.
(5) Ganoderma applanatum (Pers.) Pat is cultivated
Cultivation, manually builds booth, whole furrow, ripe bacterium rod cultured in (4) is moved into booth, carries out wall fruiting；
Go out sesame management, in the growth course of Ganoderma applanatum (Pers.) Pat, keep growing temperatures 25 DEG C～30 DEG C, relative air humidity 85%～90%, and ventilation；
Ganoderma applanatum (Pers.) Pat is gathered in the crops, and when white growth circle in cap edge disappears, cap color is pitchy, can gather during color even.
Preferably, in described step (3), broad-leaved weed tree sawdust is chestnut, fruit tree, sawdust, and culture medium raw material percentage by weight consists of cotton seed hulls 39%, corn cob 20%, weed tree sawdust 28%, wheat bran 8%, Calx 1%, Gypsum Fibrosum 1%, sugared 1% Radix Angelicae Dahuricae 1%, Camphora 1%.
Preferably, in described step (4), Cultivar culture medium raw material weight percentage ratio consists of cotton seed hulls 40%, corn cob 20%, weed tree sawdust 28%, wheat bran 8%, Calx 1%, Gypsum Fibrosum 1%, the Radix Angelicae Dahuricae 1%, Camphora 1%.
Preferably, the wall fruiting in described step (5) refers to maturation bacterium rod code is become wall, stays 2cm space between bag and bag, puts height and is advisable with 5～7 floor heights, and the soil of every mu of area can cultivate 25000 bags.
Select technique scheme provide the benefit that: adopt this method and step cultivation Ganoderma applanatum (Pers.) Pat, selection by wild Ganoderma applanatum (Pers.) Pat mother plant domestication Ganoderma applanatum (Pers.) Pat new varieties mycelia pure white dense, neat in edge；Cap is semicircle, capping pitchy, cap size 9.18cm × 6.14cm, thick 1.5～2.5cm；Stem is short, is 0.40～0.50cm, diameter 2.4～2.6cm；Yield is high, dry sesame biological efficiency more than 10%；Fruitbody polysaccharide content is high, reaches 2101.064 μ g/g, has good medicinal health value and ornamental value.The composite cultivation substrate formula simultaneously optimized takes full advantage of local forestry leftover bits and pieces and stalk resource, reduces production cost, increases economic efficiency further.The present invention can cultivate the Ganoderma applanatum (Pers.) Pat sporophore of high-quality, high yield in a word, and have cost operation low, easy, can be widely used in Ganoderma applanatum (Pers.) Pat extensive artificial and produce, it is achieved the sustainable use of Ganoderma applanatum (Pers.) Pat natural resources.
Detailed description of the invention
Below technical scheme is illustrated, in order to those skilled in the art understand.
The cultural method of a kind of Ganoderma applanatum (Pers.) Pat, described method includes preparation, original seed and the preparation of cultigen, mycelia culture, the Ganoderma applanatum (Pers.) Pat cultivation that strain selects, mother plants.
(1) strain selects:
Choose wild Ganoderma applanatum (Pers.) Pat as kind of a source, extract the parent in wild Ganoderma applanatum (Pers.) Pat, through the acclimatization culture to parent, by the Ganoderma applanatum (Pers.) Pat strain of the former parent acclimatization culture Cheng Xin of wild Ganoderma applanatum (Pers.) Pat.
(2) female preparation planted:
Preparation expansion mother plant, addition PDA culture medium in test tube, accesses the Ganoderma applanatum (Pers.) Pat strain in above-mentioned (1) after sterilizing cooling, and cultivation temperature is 25 DEG C～30 DEG C, and cultivation 7d mycelia covers with media surface becomes ripe female kind.
(3) preparation of original seed:
Selecting materials and get the raw materials ready, choosing cotton seed hulls, corn cob and weed tree sawdust is cultivation matrix, dries standby；
Prepared by original seed, the culture medium configured is loaded in original seeds bottle, autoclaving 1.5kg/cm2 keeps 3h, it is cooled to less than 30 DEG C female kind ripe in above-mentioned (2) aseptically inoculated, being placed under 20 DEG C～25 DEG C conditions lucifuge and cultivate, mycelia covers with original seeds bottle becomes ripe original seed.
(4) preparation of cultigen:
Cultivar culture medium not sugaring; the same pedigree seed culture medium of all the other proportionings; cultivating bag adopts 17cm × 39cm polypropylene plastics pocket; atmospheric steam sterilizing is carried out in time after pack; maintain 12h when temperature reaches 100 DEG C, after truce, stewing 12h can take the dish out of the pot the bag that dries in the air again, treats that pocket temperature is down to less than 28 DEG C and is aseptically inoculated by maturation original seed in above-mentioned (3); cultigen condition of culture is identical with Primary spawn condition, and mycelia becomes ripe bacterium rod after covering with cultivating bag.
(5) Ganoderma applanatum (Pers.) Pat is cultivated:
Cultivation, manually builds booth, whole furrow, ripe bacterium rod cultured in (4) is moved into booth, carries out wall fruiting；
Go out sesame management, in the growth course of Ganoderma applanatum (Pers.) Pat, keep growing temperatures 25 DEG C～30 DEG C, relative air humidity 85%～90%, and ventilation；
Ganoderma applanatum (Pers.) Pat is gathered in the crops, and when cap is no longer grown up, without white growing point, can gather during color even in edge.
In described step (3), broad-leaved weed tree sawdust is chestnut, fruit tree, sawdust etc., and culture medium raw material percentage by weight consists of cotton seed hulls 39%, corn cob 20%, weed tree sawdust 28%, wheat bran 8%, Calx 1%, Gypsum Fibrosum 1%, sugar 1%, the Radix Angelicae Dahuricae 1%, Camphora 1%；In step (4), Cultivar culture medium raw material weight percentage ratio consists of cotton seed hulls 40%, corn cob 20%, weed tree sawdust 28%, wheat bran 8%, Calx 1%, Gypsum Fibrosum 1%, the Radix Angelicae Dahuricae 1%, Camphora 1%；Wall fruiting in step (5) refers to maturation bacterium rod code is become wall, stays 2cm space between bag and bag, puts height and is advisable with 5～7 floor heights, and the soil of every mu of area can cultivate 25000 bags.
The Ganoderma applanatum (Pers.) Pat of cultivation, new varieties mycelia is pure white dense, neat in edge；Cap semicircle or subcircular, capping pitchy, diameter 6～10cm, thick 1.5～2.5cm；Stem is short, is 0.40～0.50cm, diameter 2.4～2.6cm；Yield is high, dry sesame biological efficiency more than 10%；Fruitbody polysaccharide content is high, reaches 2101.064 μ g/g, has good medicinal health value and ornamental value.
The present invention is further illustrated by above-mentioned specific embodiment, but this example does not limit the scope of the invention.In addition any change that the present invention is done by those of ordinary skill in the related art, without departing from the essence of the present invention, all drops on equivalence in claims of the present invention limited range.
Claims (3)
1. the cultural method of a Ganoderma applanatum (Pers.) Pat, it is characterised in that: described method includes preparation, original seed and the preparation of cultigen, mycelia culture, the Ganoderma applanatum (Pers.) Pat cultivation that strain selects, mother plants, and sequentially includes the following steps:
(1) strain selects:
Choose wild Ganoderma applanatum (Pers.) Pat as kind of a source, extract the parent in wild Ganoderma applanatum (Pers.) Pat, through the acclimatization culture to parent, by the Ganoderma applanatum (Pers.) Pat strain of the former parent acclimatization culture Cheng Xin of wild Ganoderma applanatum (Pers.) Pat；
(2) female preparation planted:
Preparation expands mother's kind, adds PDA culture medium, access the Ganoderma applanatum (Pers.) Pat strain in above-mentioned (1) after sterilizing cooling in test tube, and cultivation temperature is 25 DEG C～30 DEG C, cultivates 7-10 days, and mycelia covers with media surface becomes ripe female kind；
(3) preparation of original seed:
Selecting materials and get the raw materials ready, choosing cotton seed hulls, corn cob and weed tree sawdust is cultivation matrix, dries standby；
Prepared by original seed, the culture medium configured is loaded in original seeds bottle, autoclaving 1.5kg/cm2 keeps 3h, it is cooled to less than 30 DEG C female kind ripe in above-mentioned (2) aseptically inoculated, being placed under 20 DEG C～25 DEG C conditions lucifuge and cultivate, mycelia covers with original seeds bottle becomes ripe original seed；
(4) preparation of cultigen:
Cultivar culture medium not sugaring; the same pedigree seed culture medium of all the other proportionings; cultivating bag adopts 17cm × 39cm polypropylene plastics pocket; atmospheric steam sterilizing is carried out in time after pack; maintain 12h when temperature reaches 100 DEG C, after truce, stewing 12h can take the dish out of the pot the bag that dries in the air again, treats that pocket temperature is down to less than 28 DEG C and is aseptically inoculated by maturation original seed in above-mentioned (3); cultigen condition of culture is identical with Primary spawn condition, and mycelia becomes ripe bacterium rod after covering with cultivating bag；
(5) Ganoderma applanatum (Pers.) Pat is cultivated:
Cultivation, manually builds booth, whole furrow, ripe bacterium rod cultured in (4) is moved into booth, carries out wall fruiting；
Go out sesame management, in the growth course of Ganoderma applanatum (Pers.) Pat, keep growing temperatures 25 DEG C～30 DEG C, relative air humidity 85%～90%, and ventilation；
Ganoderma applanatum (Pers.) Pat is gathered in the crops, and when cap is no longer grown up, without white growing point, can gather during color even in edge.
2. the cultural method of described a kind of Ganoderma applanatum (Pers.) Pat is required according to right 1, it is characterized in that: in described step (3), broad-leaved weed tree sawdust is chestnut, fruit tree, Sophora japonica L., sawdust, culture medium raw material percentage by weight consists of cotton seed hulls 39%, corn cob 20%, weed tree sawdust 28%, wheat bran 8%, Calx 1%, Gypsum Fibrosum 1%, sugar 1%, the Radix Angelicae Dahuricae 1%, Camphora 1%.
3. the cultural method of described a kind of Ganoderma applanatum (Pers.) Pat is required according to right 1, it is characterised in that: in described step (4), Cultivar culture medium raw material weight percentage ratio consists of cotton seed hulls 40%, corn cob 20%, weed tree sawdust 28%, wheat bran 8%, Calx 1%, Gypsum Fibrosum 1%, the Radix Angelicae Dahuricae 1%, Camphora 1%.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244464A (en) * | 2016-08-15 | 2016-12-21 | 淄博寿康农业科技发展有限公司 | The cultural method of Ganderma lucidum strain |
| CN106748064A (en) * | 2016-12-08 | 2017-05-31 | 河池市技术开发中心 | It is a kind of to promote culture medium of elegant precious mushroom growth and preparation method thereof |
| CN107318460A (en) * | 2017-07-04 | 2017-11-07 | 金寨县云凡绿色中药材有限责任公司 | A kind of ecology planting method of ganoderma lucidum |
| CN109006163A (en) * | 2018-07-03 | 2018-12-18 | 沈阳农业大学 | A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method |
| CN109156262A (en) * | 2018-08-28 | 2019-01-08 | 江阴市锦杉灵芝种植专业合作社 | A kind of cultural method of high triterpene ganoderma lucidum |
| CN110115196A (en) * | 2019-05-22 | 2019-08-13 | 吉林大学 | A kind of ganoderma lucidum cultivation method |
| CN111788988A (en) * | 2020-05-19 | 2020-10-20 | 石狮昌隆农业种植有限公司 | Method and device for efficiently and artificially cultivating lucid ganoderma |
| CN111955282A (en) * | 2020-08-25 | 2020-11-20 | 广西民族师范学院 | Ecological efficient planting method for subtropical ganoderma lucidum |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244464A (en) * | 2016-08-15 | 2016-12-21 | 淄博寿康农业科技发展有限公司 | The cultural method of Ganderma lucidum strain |
| CN106748064A (en) * | 2016-12-08 | 2017-05-31 | 河池市技术开发中心 | It is a kind of to promote culture medium of elegant precious mushroom growth and preparation method thereof |
| CN107318460A (en) * | 2017-07-04 | 2017-11-07 | 金寨县云凡绿色中药材有限责任公司 | A kind of ecology planting method of ganoderma lucidum |
| CN109006163A (en) * | 2018-07-03 | 2018-12-18 | 沈阳农业大学 | A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method |
| CN109156262A (en) * | 2018-08-28 | 2019-01-08 | 江阴市锦杉灵芝种植专业合作社 | A kind of cultural method of high triterpene ganoderma lucidum |
| CN110115196A (en) * | 2019-05-22 | 2019-08-13 | 吉林大学 | A kind of ganoderma lucidum cultivation method |
| CN110115196B (en) * | 2019-05-22 | 2022-07-01 | 吉林大学 | Lucid ganoderma cultivation method |
| CN111788988A (en) * | 2020-05-19 | 2020-10-20 | 石狮昌隆农业种植有限公司 | Method and device for efficiently and artificially cultivating lucid ganoderma |
| CN111955282A (en) * | 2020-08-25 | 2020-11-20 | 广西民族师范学院 | Ecological efficient planting method for subtropical ganoderma lucidum |
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Inventor after: Lan Yufei Inventor after: Wang Qingwu Inventor after: An Xiurong Inventor after: Wei Chengxiang Inventor after: Tang Lina Inventor after: Li Xiumei Inventor after: Yu Qingwei Inventor after: Cong Qianqian Inventor after: Cui Xiao Inventor before: Lan Yufei |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160629 |