CN104641938A - Quasi wild ganoderma lipsiense cultivation process - Google Patents
Quasi wild ganoderma lipsiense cultivation process Download PDFInfo
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Abstract
The invention discloses a quasi wild ganoderma lipsiense cultivation process. According to the quasi wild ganoderma lipsiense cultivation process, the cultivation of quasi wild ganoderma lipsiense is realized through mother strain acclimatization and culture, original strain acclimatization and culture, product mycelium culture, ganoderma lipsiense mycelium culture, ganoderma lipsiense sporocarp culture and conidial powder collection. The quasi wild ganoderma lipsiense cultivation process has the beneficial effects that a wild ganoderma lipsiense strain is acclimatized into a quasi wild ganoderma lipsiense strain which can be subjected to artificial cultivation through an acclimatizing process; the cultured quasi wild ganoderma lipsiense has the effects of the wild ganoderma lipsiense; the yield of the quasi wild ganoderma lipsiense can be improved; a large amount of quasi wild ganoderma lipsiense conidial powder can be collected; the revenue of the cultivation of the quasi wild ganoderma lipsiense is improved; the economic benefits are improved; the market requirement is met; the quasi wild ganoderma lipsiense cultivation process is suitable for large-batch artificial cultivation and is favorable to popularization.
Description
Technical field
The present invention relates to Ganodermataceae technical field of cultivation, particularly relate to a kind of accurate wild ganoderma lipsiense planting technique.
Background technology
Ganoderma lipsiense is also known as the flat glossy ganoderma of artist's conk, one's old mother bacterium, maple bacterium, and fruit body is large-scale or super-huge, stockless or almost stockless, and cap is semicircle, flat hemispherical or flat, surperficial grey, gradual change brown, has concentricity rib, sometimes have knurl, cot glue cutin, edge is thinner.The shallow maroon of bacterial context, bacterium hole circle shape, being born in the snag of the broad-leaved trees such as poplar, birch, willow, oak, falling in wood and stump, is one of important wood decay fungi, and timber xylem can be caused to form white rot, pharmaceutically acceptable, among the people as cancer therapy drug at China and Japan.The moist pulmonary tuberculosis of hearsay can also be controlled, have pain relieving, heat-clearing, eliminate indigestion, hemostasis, clears phlegm.
Because wild ganoderma lipsiense output is few, effective, therefore expensive, and the ganoderma lipsiense of artificial commonsense method cultivation lacks effect that wild ganoderma lipsiense has, although output adds, the ganoderma lipsiense effect of artificial commonsense method cultivation does not reach the effect of wild ganoderma lipsiense completely, therefore, develop effect that a kind of ganoderma lipsiense cultivated both had had wild ganoderma lipsiense, the ganoderma lipsiense planting technique that can improve again ganoderma lipsiense output is that market needed badly.
Summary of the invention
The present invention will solve above-mentioned prior art Problems existing, a kind of accurate wild ganoderma lipsiense planting technique is provided, by by wild ganoderma lipsiense strain domestication, again by domestication after wild ganoderma lipsiense bacterial classification cultivate, the wild ganoderma lipsiense of the standard cultivated both had had effect of wild ganoderma lipsiense, the output of ganoderma lipsiense can be improved again, meet the demand in market.
The present invention solves the technical scheme that its technical problem adopts: the wild ganoderma lipsiense planting technique of this standard, and it is undertaken by the following step:
The first step, female kind tames cultivation, choose tri-distilled water, corn flour, land plaster, leaf of elm tree adopts culture dish to be made into mother culture media, be dispensed into again in test tube, wherein the consumption of tri-distilled water is 1kg ~ 3kg, the consumption of corn flour is 7kg ~ 9kg, the consumption of leaf of elm tree is 0.5kg ~ 0.8kg, the content of land plaster in mother culture media is 0.2%, wherein the process of leaf of elm tree obtains leaf of elm tree powder after leaf of elm tree crushing and screening is put 70 order ~ 90 orders, 1.8Mpa pressure autoclaving 60 minutes, after cooling, mother culture media test tube and wild ganoderma lipsiense are put into inoculating hood sterilization 40 minutes, then choose mycelia that wild ganoderma lipsiense is grown on trees place to be linked in mother culture media as bacterial classification and to carry out first time and tame cultivation, the mother's kind after domestication is obtained after mycelium germination,
Second step, original seed domestication is cultivated, choose tri-distilled water, corn flour, paddy, ginkgo leaf, land plaster adopts culture dish to be made into pedigree seed culture medium, wherein tri-distilled water, corn flour, paddy, ginkgo leaf, the content ratio of land plaster is 1:1:1.5:2:0.2, the process of paddy is boiled by life paddy to shell to ftracture, the process of ginkgo leaf obtains ginkgo leaf powder after ginkgo leaf crushing and screening is put 60 order ~ 70 orders, 1.5Mpa pressure autoclaving 3 hours, carry out disinfection again after cooling, after sterilization, the mother of acquisition is planted to be linked in pedigree seed culture medium and carry out second time and tame cultivation, namely the original seed after domestication is obtained after mycelium germination,
3rd step, produce kind of a cultural hypha, choose tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, maple leaves adopts culture dish to be made into and produces kind of a medium, wherein tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, the content ratio of maple leaves is 1:1:2:2:0.2:3, wherein the process of maple leaves obtains maple leaves powder after maple leaves crushing and screening is put 80 order ~ 100 orders, sterilizing 10 hours at 100 DEG C of temperature, carry out disinfection again after cooling, after sterilization, the production kind of acquisition is linked into produce in kind of medium and cultivates, namely the production kind bacterial classification after domestication is obtained after mycelium germination,
4th step, ganoderma lipsiense mycelia is cultivated, choosing arbor, to be made into diameter be 3cm ~ 5cm, length is the linden of 35cm ~ 45cm, then 3 ~ 5 inoculation mouths are got respectively at the two ends of linden, then be one be assembled in high density polyethylene (HDPE) bacterium bag by linden with three, again the bacterium bag installed is carried out high-temperature steam sterilization processing, sterilising temp is 97 DEG C ~ 100 DEG C, sterilization time is 8 hours ~ 11 hours, after bacterium bag naturally cools to normal temperature, production kind bacterial classification after domestication is linked into the linden two ends of bacterium bag, the booth that ventilation is good is moved into after inoculation, cultural hypha is carried out in shaded place or air conditioning chamber, the temperature of cultivating is 23 DEG C ~ 25 DEG C, relative moisture is 68% ~ 74%, till mycelia covers with bacterium bag,
5th step, ganoderma lipsiense fruit body is cultivated, and is transported into by the bacterium bag covering with mycelia in fully stocked wood, the broad-leaved mountain forest ground that shelters from heat or light and imbeds in humus soil the cultivation carrying out accurate wild ganoderma lipsiense fruit body again, and when imbedding, half bacterium bag dew is on the ground;
6th step, the collection of conidial powder, the collection of conidial powder is carried out when ganoderma lipsiense fruit body maturity reaches 70% ~ 75%, nylon paper is first got in side openings during collection, then stem nylon paper being passed ganoderma lipsiense fruit body is layered on the ground below ganoderma lipsiense fruit body cap, then nylon paper is adopted to put ganoderma lipsiense fruit body on the spot all around, then blank sheet of paper on covering on the top of ganoderma lipsiense fruit body, conidial powder drops on nylon paper, the conidial powder dropped on nylon paper is collected.
Further, wood chip is made up of wooden grain, and the size of wooden grain is 1.5cm*1.5cm*1.5cm.
The wild ganoderma lipsiense planting technique of standard of the present invention, as bacterial classification in the first step using wild ganoderma lipsiense, then cultivated by mother culture media and carry out first time domestication, the object adding leaf of elm tree in first time domestication is the growing environment of being simulated wild ganoderma lipsiense by leaf of elm tree, reduce wild ganoderma lipsiense bacterial classification to the resistance of artificial environment, wild ganoderma lipsiense bacterial classification is successfully tamed, choose the mycelia that wild ganoderma lipsiense is grown on trees place and can improve strain domestication success rate as bacterial classification, wild ganoderma lipsiense bacterial classification is tamed smoothly, in second step be using cultivate after mother plant as bacterial classification, then cultivated by pedigree seed culture medium and carry out second time domestication, the object adding ginkgo leaf in second time domestication is the growing environment of being simulated wild ganoderma lipsiense by ginkgo leaf further, because ginkgo leaf is different from the leaf of elm tree in the first step, the effect of the wild living environment of planization can be reached, thus allow the wild ganoderma lipsiense of taming through first time can not resist artificial culture environment, thus reach the object of domestication further, in 3rd step be using cultivate after original seed as bacterial classification, then third time domestication is carried out by producing kind of a medium culture, the maple leaves added in the third step and ginkgo leaf, leaf of elm tree is also different, and the object adding maple leaves is also the wild living environment of simulation, reduce the resistance to artificial environment of wild ganoderma lipsiense bacterial classification, thus wild ganoderma lipsiense bacterial classification can be allowed to tame completely become can by tame growth kind, the bacterial classification that third time has tamed rear acquisition just can carry out artificial cultivation as production kind, being changed over by the bacterial classification of wild ganoderma lipsiense by domestication step can by the wild ganoderma lipsiense production kind of tame standard, pass through the first step, second step, the domestication incubation of the 3rd step makes wild ganoderma lipsiense bacterial classification change the wild ganoderma lipsiense bacterial classification that is as the criterion, effect of wild ganoderma lipsiense can either be retained, have again and can carry out tame ability, 4th step and the 5th step are produced by wild for standard ganoderma lipsiense to plant to carry out cultural hypha and the cultivation carrying out accurate wild ganoderma lipsiense fruit body, because the wild ganoderma lipsiense of standard can adapt to artificial culture environment completely in the process of domestication, and the accreditation remained wild living environment, therefore, directly just wild for standard ganoderma lipsiense can be carried out the cultivation of fruit body, do not need to conform again, reduce the cultivation that some are unnecessary, save planting time, in the production kind medium of the 3rd step, the object adding maple leaves is to simulate grows wild environment, allow wild ganoderma lipsiense bacterial classification can retain effect of wild ganoderma lipsiense in domestication process, the mycelia vitality of planting is produced strong after adding maple leaves, growth fast, be beneficial to the cultivation of accurate wild ganoderma lipsiense, at mother culture media, pedigree seed culture medium, produce in kind of medium that to add land plaster be toughness in order to increase mycelia and viscosity, be convenient to mycelia grow in linden, the collection of conidial powder is carried out in the 6th step, containing a large amount of active ingredient in conidial powder, adopt the 6th one step process can prevent conidial powder from dropping in the environment of surrounding, conidial powder is collected on nylon paper, the output of conidial powder can be improved, improve the income of the accurate wild ganoderma lipsiense of cultivation, improve productivity effect, in the production kind medium of the 3rd step, wood chip adopts wooden grain to form, and the size of wooden grain is 1.5cm*1.5cm*1.5cm, length, width and height are respectively 1.5cm, accurate wild ganoderma lipsiense bacterial classification can be convenient to like this inoculate, in the present invention, female kind, original seed, what production kind referred to is all bacterial classification, the effect of high-temperature steam is not only sterilization, and be the resistance of arbor to kill, so that the linden that arbor is made is transformed into the state of rotten wood, because wild ganoderma lipsiense only grows on rotten wood, this makes it possible to the growing environment of simulating wild ganoderma lipsiense, be convenient to the growth of accurate wild ganoderma lipsiense, the shape of linden is made fan-shaped, such three lindens just constitute a cylinder, be convenient to pack, because the active constituent content in conidial powder is higher than the content of active ingredient in ganoderma lipsiense, therefore, the collection of conidial powder can improve the benefit of overall accurate wild ganoderma lipsiense.
The effect that the present invention is useful is: the wild ganoderma lipsiense planting technique of standard of the present invention, by domestication process, wild ganoderma lipsiense strain domestication is become can carry out the wild ganoderma lipsiense bacterial classification of tame standard, the wild ganoderma lipsiense of the standard cultivated both had had effect of wild ganoderma lipsiense, the output of accurate wild ganoderma lipsiense can be improved again, be applicable to artificial a large amount of cultivation, on the basis retaining wild ganoderma lipsiense effect, add the cultivation quantity of accurate wild ganoderma lipsiense, the planting time of accurate wild ganoderma lipsiense can also be shortened, and accurate wild ganoderma lipsiense conidial powder can be collected in a large number, improve the income of cultivating accurate wild ganoderma lipsiense, improve economic benefit, meet the demand in market, be beneficial to popularization.
Embodiment
The invention will be further described below:
Embodiment one:
The wild ganoderma lipsiense planting technique of this standard, it is undertaken by the following step:
The first step, female kind tames cultivation, choose tri-distilled water, corn flour, land plaster, leaf of elm tree adopts culture dish to be made into mother culture media, be dispensed into again in test tube, wherein the consumption of tri-distilled water is 1kg, the consumption of corn flour is 7kg, the consumption of leaf of elm tree is 0.5kg, the content of land plaster in mother culture media is 0.2%, wherein the process of leaf of elm tree obtains leaf of elm tree powder after leaf of elm tree crushing and screening is put 70 orders, 1.8Mpa pressure autoclaving 60 minutes, after cooling, mother culture media test tube and wild ganoderma lipsiense are put into inoculating hood sterilization 40 minutes, then choose mycelia that wild ganoderma lipsiense is grown on trees place to be linked in mother culture media as bacterial classification and to carry out first time and tame cultivation, the mother's kind after domestication is obtained after mycelium germination,
Second step, original seed domestication is cultivated, choose tri-distilled water, corn flour, paddy, ginkgo leaf, land plaster adopts culture dish to be made into pedigree seed culture medium, wherein tri-distilled water, corn flour, paddy, ginkgo leaf, the content ratio of land plaster is 1:1:1.5:2:0.2, the process of paddy is boiled by life paddy to shell to ftracture, the process of ginkgo leaf obtains ginkgo leaf powder after ginkgo leaf crushing and screening is put 60 orders, 1.5Mpa pressure autoclaving 3 hours, carry out disinfection again after cooling, after sterilization, the mother of acquisition is planted to be linked in pedigree seed culture medium and carry out second time and tame cultivation, namely the original seed after domestication is obtained after mycelium germination,
3rd step, produce kind of a cultural hypha, choose tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, maple leaves adopts culture dish to be made into and produces kind of a medium, wherein tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, the content ratio of maple leaves is 1:1:2:2:0.2:3, wherein the process of maple leaves obtains maple leaves powder after maple leaves crushing and screening is put 80 orders, sterilizing 10 hours at 100 DEG C of temperature, carry out disinfection again after cooling, after sterilization, the production kind of acquisition is linked into produce in kind of medium and cultivates, namely the production kind bacterial classification after domestication is obtained after mycelium germination,
4th step, ganoderma lipsiense mycelia is cultivated, choosing arbor, to be made into diameter be 3cm, length is the linden of 35cm, then 3 inoculation mouths are got respectively at the two ends of linden, then be one be assembled in high density polyethylene (HDPE) bacterium bag by linden with three, again the bacterium bag installed is carried out high-temperature steam sterilization processing, sterilising temp is 97 DEG C, sterilization time is 8 hours, after bacterium bag naturally cools to normal temperature, production kind bacterial classification after domestication is linked into the linden two ends of bacterium bag, the booth that ventilation is good is moved into after inoculation, cultural hypha is carried out in shaded place or air conditioning chamber, the temperature of cultivating is 23 DEG C, relative moisture is 68%, till mycelia covers with bacterium bag,
5th step, ganoderma lipsiense fruit body is cultivated, and is transported into by the bacterium bag covering with mycelia in fully stocked wood, the broad-leaved mountain forest ground that shelters from heat or light and imbeds in humus soil the cultivation carrying out accurate wild ganoderma lipsiense fruit body again, and when imbedding, half bacterium bag dew is on the ground;
6th step, the collection of conidial powder, the collection of conidial powder is carried out when ganoderma lipsiense fruit body maturity reaches 70%, nylon paper is first got in side openings during collection, then stem nylon paper being passed ganoderma lipsiense fruit body is layered on the ground below ganoderma lipsiense fruit body cap, then nylon paper is adopted to put ganoderma lipsiense fruit body on the spot all around, then blank sheet of paper on covering on the top of ganoderma lipsiense fruit body, conidial powder drops on nylon paper, the conidial powder dropped on nylon paper is collected.
Further, wood chip is made up of wooden grain, and the size of wooden grain is 1.5cm*1.5cm*1.5cm.
Embodiment two:
The wild ganoderma lipsiense planting technique of this standard, it is undertaken by the following step:
The first step, female kind tames cultivation, choose tri-distilled water, corn flour, land plaster, leaf of elm tree adopts culture dish to be made into mother culture media, be dispensed into again in test tube, wherein the consumption of tri-distilled water is 2kg, the consumption of corn flour is 8kg, the consumption of leaf of elm tree is 0.6kg, the content of land plaster in mother culture media is 0.2%, wherein the process of leaf of elm tree obtains leaf of elm tree powder after leaf of elm tree crushing and screening is put 70 order ~ 90 orders, 1.8Mpa pressure autoclaving 60 minutes, after cooling, mother culture media test tube and wild ganoderma lipsiense are put into inoculating hood sterilization 40 minutes, then choose mycelia that wild ganoderma lipsiense is grown on trees place to be linked in mother culture media as bacterial classification and to carry out first time and tame cultivation, the mother's kind after domestication is obtained after mycelium germination,
Second step, original seed domestication is cultivated, choose tri-distilled water, corn flour, paddy, ginkgo leaf, land plaster adopts culture dish to be made into pedigree seed culture medium, wherein tri-distilled water, corn flour, paddy, ginkgo leaf, the content ratio of land plaster is 1:1:1.5:2:0.2, the process of paddy is boiled by life paddy to shell to ftracture, the process of ginkgo leaf obtains ginkgo leaf powder after ginkgo leaf crushing and screening is put 65 orders, 1.5Mpa pressure autoclaving 3 hours, carry out disinfection again after cooling, after sterilization, the mother of acquisition is planted to be linked in pedigree seed culture medium and carry out second time and tame cultivation, namely the original seed after domestication is obtained after mycelium germination,
3rd step, produce kind of a cultural hypha, choose tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, maple leaves adopts culture dish to be made into and produces kind of a medium, wherein tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, the content ratio of maple leaves is 1:1:2:2:0.2:3, wherein the process of maple leaves obtains maple leaves powder after maple leaves crushing and screening is put 90 orders, sterilizing 10 hours at 100 DEG C of temperature, carry out disinfection again after cooling, after sterilization, the production kind of acquisition is linked into produce in kind of medium and cultivates, namely the production kind bacterial classification after domestication is obtained after mycelium germination,
4th step, ganoderma lipsiense mycelia is cultivated, choosing arbor, to be made into diameter be 4cm, length is the linden of 40cm, then 4 inoculation mouths are got respectively at the two ends of linden, then be one be assembled in high density polyethylene (HDPE) bacterium bag by linden with three, again the bacterium bag installed is carried out high-temperature steam sterilization processing, sterilising temp is 98 DEG C, sterilization time is 9 hours, after bacterium bag naturally cools to normal temperature, production kind bacterial classification after domestication is linked into the linden two ends of bacterium bag, the booth that ventilation is good is moved into after inoculation, cultural hypha is carried out in shaded place or air conditioning chamber, the temperature of cultivating is 24 DEG C, relative moisture is 70%, till mycelia covers with bacterium bag,
5th step, ganoderma lipsiense fruit body is cultivated, and is transported into by the bacterium bag covering with mycelia in fully stocked wood, the broad-leaved mountain forest ground that shelters from heat or light and imbeds in humus soil the cultivation carrying out accurate wild ganoderma lipsiense fruit body again, and when imbedding, half bacterium bag dew is on the ground;
6th step, the collection of conidial powder, the collection of conidial powder is carried out when ganoderma lipsiense fruit body maturity reaches 72%, nylon paper is first got in side openings during collection, then stem nylon paper being passed ganoderma lipsiense fruit body is layered on the ground below ganoderma lipsiense fruit body cap, then nylon paper is adopted to put ganoderma lipsiense fruit body on the spot all around, then blank sheet of paper on covering on the top of ganoderma lipsiense fruit body, conidial powder drops on nylon paper, the conidial powder dropped on nylon paper is collected.
Further, wood chip is made up of wooden grain, and the size of wooden grain is 1.5cm*1.5cm*1.5cm.
Embodiment three:
The wild ganoderma lipsiense planting technique of this standard, it is undertaken by the following step:
The first step, female kind tames cultivation, choose tri-distilled water, corn flour, land plaster, leaf of elm tree adopts culture dish to be made into mother culture media, be dispensed into again in test tube, wherein the consumption of tri-distilled water is 3kg, the consumption of corn flour is 9kg, the consumption of leaf of elm tree is 0.8kg, the content of land plaster in mother culture media is 0.2%, wherein the process of leaf of elm tree obtains leaf of elm tree powder after leaf of elm tree crushing and screening is put 90 orders, 1.8Mpa pressure autoclaving 60 minutes, after cooling, mother culture media test tube and wild ganoderma lipsiense are put into inoculating hood sterilization 40 minutes, then choose mycelia that wild ganoderma lipsiense is grown on trees place to be linked in mother culture media as bacterial classification and to carry out first time and tame cultivation, the mother's kind after domestication is obtained after mycelium germination,
Second step, original seed domestication is cultivated, choose tri-distilled water, corn flour, paddy, ginkgo leaf, land plaster adopts culture dish to be made into pedigree seed culture medium, wherein tri-distilled water, corn flour, paddy, ginkgo leaf, the content ratio of land plaster is 1:1:1.5:2:0.2, the process of paddy is boiled by life paddy to shell to ftracture, the process of ginkgo leaf obtains ginkgo leaf powder after ginkgo leaf crushing and screening is put 70 orders, 1.5Mpa pressure autoclaving 3 hours, carry out disinfection again after cooling, after sterilization, the mother of acquisition is planted to be linked in pedigree seed culture medium and carry out second time and tame cultivation, namely the original seed after domestication is obtained after mycelium germination,
3rd step, produce kind of a cultural hypha, choose tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, maple leaves adopts culture dish to be made into and produces kind of a medium, wherein tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, the content ratio of maple leaves is 1:1:2:2:0.2:3, wherein the process of maple leaves obtains maple leaves powder after maple leaves crushing and screening is put 100 orders, sterilizing 10 hours at 100 DEG C of temperature, carry out disinfection again after cooling, after sterilization, the production kind of acquisition is linked into produce in kind of medium and cultivates, namely the production kind bacterial classification after domestication is obtained after mycelium germination,
4th step, ganoderma lipsiense mycelia is cultivated, choosing arbor, to be made into diameter be 5cm, length is the linden of 45cm, then 5 inoculation mouths are got respectively at the two ends of linden, then be one be assembled in high density polyethylene (HDPE) bacterium bag by linden with three, again the bacterium bag installed is carried out high-temperature steam sterilization processing, sterilising temp is 100 DEG C, sterilization time is 11 hours, after bacterium bag naturally cools to normal temperature, production kind bacterial classification after domestication is linked into the linden two ends of bacterium bag, the booth that ventilation is good is moved into after inoculation, cultural hypha is carried out in shaded place or air conditioning chamber, the temperature of cultivating is 25 DEG C, relative moisture is 74%, till mycelia covers with bacterium bag,
5th step, ganoderma lipsiense fruit body is cultivated, and is transported into by the bacterium bag covering with mycelia in fully stocked wood, the broad-leaved mountain forest ground that shelters from heat or light and imbeds in humus soil the cultivation carrying out accurate wild ganoderma lipsiense fruit body again, and when imbedding, half bacterium bag dew is on the ground;
6th step, the collection of conidial powder, the collection of conidial powder is carried out when ganoderma lipsiense fruit body maturity reaches 75%, nylon paper is first got in side openings during collection, then stem nylon paper being passed ganoderma lipsiense fruit body is layered on the ground below ganoderma lipsiense fruit body cap, then nylon paper is adopted to put ganoderma lipsiense fruit body on the spot all around, then blank sheet of paper on covering on the top of ganoderma lipsiense fruit body, conidial powder drops on nylon paper, the conidial powder dropped on nylon paper is collected.
Further, wood chip is made up of wooden grain, and the size of wooden grain is 1.5cm*1.5cm*1.5cm.
Choose 100 parts of embodiment of the present invention one, the wild ganoderma lipsiense of standard that embodiment two, embodiment three are cultivated and wild ganoderma lipsiense, the booth manual method ganoderma lipsiense of cultivating contrast, concrete outcome is as follows:
The ganoderma lipsiense that the wild ganoderma lipsiense of standard of the embodiment of the present invention one, embodiment two, embodiment three being cultivated again and wild ganoderma lipsiense, booth manual method are cultivated carries out the contrast in production efficiency, and comparing result is as follows:
From two comparing results above, the wild ganoderma lipsiense of standard of the embodiment of the present invention, effect can reach the effect of wild ganoderma lipsiense, output time and output are also better than the ganoderma lipsiense of common manual method cultivation, and the conidial powder output collected is also far away higher than the ganoderma lipsiense that wild ganoderma lipsiense and manual method are cultivated, therefore, the standard of the embodiment of the present invention wild ganoderma lipsiense planting technique is better than common manual method.
The feature of the embodiment of the present invention is: become can carry out the wild ganoderma lipsiense bacterial classification of tame standard by wild ganoderma lipsiense strain domestication by domestication process, the wild ganoderma lipsiense of the standard cultivated both had had effect of wild ganoderma lipsiense, the output of accurate wild ganoderma lipsiense can be improved again, be applicable to artificial a large amount of cultivation, on the basis retaining wild ganoderma lipsiense effect, add the cultivation quantity of accurate wild ganoderma lipsiense, the planting time of accurate wild ganoderma lipsiense can also be shortened, and accurate wild ganoderma lipsiense conidial powder can be collected in a large number, improve the income of cultivating accurate wild ganoderma lipsiense, improve economic benefit, meet the demand in market, be beneficial to popularization.
Although the present invention is described by reference to preferred embodiment, those skilled in the art should understand, and in the scope of claims, can do the various change in form and details.
Claims (2)
1. the wild ganoderma lipsiense planting technique of standard, is characterized in that it is undertaken by the following step:
(1) female kind tames cultivation, choose tri-distilled water, corn flour, land plaster, leaf of elm tree adopts culture dish to be made into mother culture media, be dispensed into again in test tube, wherein the consumption of tri-distilled water is 1kg ~ 3kg, the consumption of corn flour is 7kg ~ 9kg, the consumption of leaf of elm tree is 0.5kg ~ 0.8kg, the content of land plaster in mother culture media is 0.2%, wherein the process of leaf of elm tree obtains leaf of elm tree powder after leaf of elm tree crushing and screening is put 70 order ~ 90 orders, 1.8Mpa pressure autoclaving 60 minutes, after cooling, mother culture media test tube and wild ganoderma lipsiense are put into inoculating hood sterilization 40 minutes, then choose mycelia that wild ganoderma lipsiense is grown on trees place to be linked in mother culture media as bacterial classification and to carry out first time and tame cultivation, the mother's kind after domestication is obtained after mycelium germination,
(2) original seed domestication is cultivated, choose tri-distilled water, corn flour, paddy, ginkgo leaf, land plaster adopts culture dish to be made into pedigree seed culture medium, wherein tri-distilled water, corn flour, paddy, ginkgo leaf, the content ratio of land plaster is 1:1:1.5:2:0.2, the process of paddy is boiled by life paddy to shell to ftracture, the process of ginkgo leaf obtains ginkgo leaf powder after ginkgo leaf crushing and screening is put 60 order ~ 70 orders, 1.5Mpa pressure autoclaving 3 hours, carry out disinfection again after cooling, after sterilization, the mother of acquisition is planted to be linked in pedigree seed culture medium and carry out second time and tame cultivation, namely the original seed after domestication is obtained after mycelium germination,
(3) kind of a cultural hypha is produced, choose tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, maple leaves adopts culture dish to be made into and produces kind of a medium, wherein tri-distilled water, wood chip, wheat bran, brown sugar, land plaster, the content ratio of maple leaves is 1:1:2:2:0.2:3, wherein the process of maple leaves obtains maple leaves powder after maple leaves crushing and screening is put 80 order ~ 100 orders, sterilizing 10 hours at 100 DEG C of temperature, carry out disinfection again after cooling, after sterilization, the production kind of acquisition is linked into produce in kind of medium and cultivates, namely the production kind bacterial classification after domestication is obtained after mycelium germination,
(4) ganoderma lipsiense mycelia cultivation, choosing arbor, to be made into diameter be 3cm ~ 5cm, length is the linden of 35cm ~ 45cm, then 3 ~ 5 inoculation mouths are got respectively at the two ends of linden, then be one be assembled in high density polyethylene (HDPE) bacterium bag by linden with three, again the bacterium bag installed is carried out high-temperature steam sterilization processing, sterilising temp is 97 DEG C ~ 100 DEG C, sterilization time is 8 hours ~ 11 hours, after bacterium bag naturally cools to normal temperature, production kind bacterial classification after domestication is linked into the linden two ends of bacterium bag, the booth that ventilation is good is moved into after inoculation, cultural hypha is carried out in shaded place or air conditioning chamber, the temperature of cultivating is 23 DEG C ~ 25 DEG C, relative moisture is 68% ~ 74%, till mycelia covers with bacterium bag,
(5) ganoderma lipsiense fruit body is cultivated, and is transported into by the bacterium bag covering with mycelia in fully stocked wood, the broad-leaved mountain forest ground that shelters from heat or light and imbeds in humus soil the cultivation carrying out accurate wild ganoderma lipsiense fruit body again, and when imbedding, half bacterium bag dew on the ground;
(6) collection of conidial powder, the collection of conidial powder is carried out when ganoderma lipsiense fruit body maturity reaches 70% ~ 75%, nylon paper is first got in side openings during collection, then stem nylon paper being passed ganoderma lipsiense fruit body is layered on the ground below ganoderma lipsiense fruit body cap, then nylon paper is adopted to put ganoderma lipsiense fruit body on the spot all around, then blank sheet of paper on covering on the top of ganoderma lipsiense fruit body, conidial powder drops on nylon paper, the conidial powder dropped on nylon paper is collected.
2. the wild ganoderma lipsiense planting technique of standard according to claim 1, is characterized in that: in described step (3), wood chip is made up of wooden grain, and the size of wooden grain is 1.5cm*1.5cm*1.5cm.
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Application publication date: 20150527 Assignee: Longquan Wild Ganoderma Research Institute Assignor: Zhejiang meidiyuan Biotechnology Co.,Ltd. Contract record no.: X2022980023335 Denomination of invention: Cultivation Methods of Quasi wild Ganoderma Lucidum Granted publication date: 20170111 License type: Common License Record date: 20221127 |