CN111955282A - Ecological efficient planting method for subtropical ganoderma lucidum - Google Patents

Abstract

The invention relates to the technical field of ganoderma lucidum planting, and discloses an ecological efficient planting method for subtropical ganoderma lucidum, wherein a wild ganoderma lucidum variety grown in subtropical indigenous area is adopted, and a culture medium for bag cultivation is prepared by taking checked wood, Baggu, mulberry twigs and bagasse which have resource advantages in subtropical area as raw materials, and the culture is classified and produced by adopting strains, wherein each grade adopts an adaptive culture medium; in the process of culturing the third-level strains of the lucid ganoderma, a first-level strain culture medium, a second-level strain culture medium, a third-level strain formula and a ground culture medium are respectively prepared, so that the requirements of different nutrients in the growth process of each level of strains are met, the problems of single tree species, unreasonable culture medium ingredients and insufficient nutrient substance accumulation when one culture medium is directly adopted are solved, and a foundation is laid for high-quality and high-yield culture of the lucid ganoderma; meanwhile, the waste bagasse is recycled, so that resources are fully utilized, and waste materials are changed into valuable materials.

Description

Ecological efficient planting method for subtropical ganoderma lucidum

Technical Field

The invention relates to the technical field of ganoderma lucidum planting, in particular to an ecological and efficient subtropical ganoderma lucidum planting method.

Background

The ganoderma lucidum is named as red ganoderma, wood ganoderma, fungus ganoderma and ganoderma lucidum, and is the traditional famous and precious edible and medicinal fungi in China. The lucid ganoderma has obvious curative effects on regulating blood sugar, controlling blood pressure, assisting tumor chemotherapy, protecting liver, promoting sleep and the like. The Ganoderma belongs to medium-high temperature wood rot fungi, and has a growth temperature of 26-28 deg.C, and is suitable for growth in tropical and subtropical regions. For example, Guangxi belongs to tropical and subtropical climates, the rain and heat resources are rich, the climate is mild, the average annual air temperature is 16-23 ℃, the annual rainfall is 1000-2800mm, the forest coverage rate is 61.4 percent, the natural climate conditions are particularly suitable for the growth and propagation of the lucid ganoderma, and most places have the tradition of cultivating the lucid ganoderma.

At the present stage, the ganoderma lucidum cultivation technology is weak, the cut-log cultivation and the wood chip bag cultivation are mainly used, the commonly used tree species are single, the cultivation material is unreasonable in ingredient and insufficient in nutrient accumulation in the bag cultivation process, the yield is low, and the product grade is not high.

Disclosure of Invention

Based on the problems, the invention provides an ecological efficient planting method of subtropical ganoderma lucidum, which is characterized in that a wild ganoderma lucidum variety grown in subtropical regions is adopted, and a culture medium for bag cultivation is prepared by taking bakken, green roughcast and mulberry twigs with resource advantages in subtropical regions as raw materials, and strains are classified and produced, wherein each grade adopts an appropriate culture medium; in the process of culturing the third-level strains of the lucid ganoderma, a first-level strain culture medium, a second-level strain culture medium, a third-level strain formula and a ground culture medium are respectively prepared, so that the requirements of different nutrients in the growth process of each level of strains are met, the problems of single tree species, unreasonable culture medium ingredients and insufficient nutrient substance accumulation when one culture medium is directly adopted are solved, and a foundation is laid for high-quality and high-yield culture of the lucid ganoderma; meanwhile, the waste bagasse is recycled, so that resources are fully utilized, and waste materials are changed into valuable materials.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

an ecological high-efficiency planting method of subtropical ganoderma lucidum comprises the following steps:

s1, weighing 10-20 parts of fresh green roughhaired holly leaves, 10-20 parts of fresh gelia japonica leaves, 20-25 parts of peptone, 30-40 parts of sugarcane juice, 20-25 parts of agar and 800 parts of water according to parts by weight, uniformly mixing the materials, and sterilizing to obtain a first-class seed culture medium; selecting a wild ganoderma variety grown in subtropical regions, inoculating a small piece of ganoderma flesh tissue on a primary culture medium by adopting a tissue isolation culture technology, and culturing for 7-10 days to obtain a primary seed;

s2, weighing 60 parts of miscellaneous wood chips, 40 parts of bagasse and 1.5 parts of gypsum powder according to parts by weight, uniformly mixing the materials, sterilizing to obtain a secondary seed culture medium, and inoculating the primary seed on the secondary seed culture medium to culture for 20-25 days to obtain a secondary seed;

s3, weighing 78 parts of the bakken wood chips, 10 parts of wheat bran, 1 part of sugar and 1 part of gypsum powder according to the parts by weight, uniformly mixing the materials, sterilizing to obtain a third-class seed culture medium, and inoculating the second-class seeds on the third-class seed culture medium to culture for 18-20 days to obtain third-class seeds;

s4, weighing 60 parts of the total of the green roughhaired holly, the mulberry twigs and the wood shavings, 38 parts of bagasse and 2 parts of gypsum powder according to the parts by weight, uniformly mixing the materials, sterilizing to obtain a ground culture compost, and then inoculating the third-level seeds on the ground culture compost to culture the lucid ganoderma.

Furthermore, the mixed wood chips comprise 50% of green-hillock wood chips and 50% of mulberry branch chips.

Compared with the prior art, the invention has the beneficial effects that: the method adopts a wild ganoderma lucidum variety grown in subtropical regions, takes the grapevine, the lappa japonica and the mulberry twig with resource superiority in the subtropical regions as raw materials to prepare the culture medium for bag cultivation, adopts strain classification and production, and adopts a suitable culture medium for each grade; in the process of culturing the third-level strains of the lucid ganoderma, a first-level strain culture medium, a second-level strain culture medium, a third-level strain formula and a ground culture medium are respectively prepared, so that the requirements of different nutrients in the growth process of each level of strains are met, the problems of single tree species, unreasonable culture medium ingredients and insufficient nutrient substance accumulation when one culture medium is directly adopted are solved, and a foundation is laid for high-quality and high-yield culture of the lucid ganoderma; meanwhile, the waste bagasse is recycled, so that resources are fully utilized, and waste materials are changed into valuable materials.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not used as limitations of the present invention.

Example (b):

an ecological high-efficiency planting method of subtropical ganoderma lucidum comprises the following steps:

s1, weighing 10-20 parts of fresh green roughhaired holly leaves, 10-20 parts of fresh gelia japonica leaves, 20-25 parts of peptone, 30-40 parts of sugarcane juice, 20-25 parts of agar and 800 parts of water according to parts by weight, uniformly mixing the materials, and sterilizing to obtain a first-class seed culture medium; selecting a wild ganoderma variety grown in subtropical regions, inoculating a small piece of ganoderma flesh tissue on a primary culture medium by adopting a tissue isolation culture technology, and culturing for 7-10 days to obtain a primary seed;

s2, weighing 60 parts of miscellaneous wood chips, 40 parts of bagasse and 1.5 parts of gypsum powder according to parts by weight, uniformly mixing the materials, sterilizing to obtain a secondary seed culture medium, and inoculating the primary seed on the secondary seed culture medium to culture for 20-25 days to obtain a secondary seed; the mixture of the miscellaneous tree wood chips in the embodiment is 50% of green hillock wood chips and 50% of mulberry branch wood chips. The bagasse is the residue left after sugar squeezing of sugarcane, and is white or yellow-white after drying, and has the fragrance of sugar, and the coarse residue with peel is crushed into particles with particle size of 4mm-5 mm. The main components of bagasse are about 50% cellulose, 30% hemicellulose, 15% lignin, and a little ash, crude protein and soluble sugars. Can provide sugar and the like for ganoderma lucidum strains, efficiently utilizes waste resources and changes waste into valuable.

S3, weighing 78 parts of the bakken wood chips, 10 parts of wheat bran, 1 part of sugar and 1 part of gypsum powder according to the parts by weight, uniformly mixing the materials, sterilizing to obtain a third-class seed culture medium, and inoculating the second-class seeds on the third-class seed culture medium to culture for 18-20 days to obtain third-class seeds;

s4, weighing 60 parts of the total of the green roughhaired holly, the mulberry twigs and the wood shavings, 38 parts of bagasse and 2 parts of gypsum powder according to the parts by weight, uniformly mixing the materials, sterilizing to obtain a ground culture compost, and then inoculating the third-level seeds on the ground culture compost to culture the lucid ganoderma.

In the embodiment, a wild ganoderma variety growing in subtropical regions is selected, and a small piece of ganoderma flesh tissue is inoculated on a primary culture medium through a tissue isolation culture technology, so that the cultured ganoderma has good genes, the yield of the ganoderma is fundamentally increased, and meanwhile, the subtropical regions suitable for the growth of the ganoderma are selected for culture, so that the ganoderma has a good growth environment; preparing culture medium for bag cultivation by using the grapevine, the green roughhaired holly and the mulberry twig which have the resource advantage in subtropical regions as raw materials, and adopting strain classification and production, wherein each grade adopts a suitable culture medium; in the process of culturing the third-level strains of the lucid ganoderma, a first-level strain culture medium, a second-level strain culture medium, a third-level strain formula and a ground culture medium are respectively prepared, so that the requirements of different nutrients in the growth process of each level of strains are met, the problems of single tree species, unreasonable culture medium ingredients and insufficient nutrient substance accumulation when one culture medium is directly adopted are solved, and a foundation is laid for high-quality and high-yield culture of the lucid ganoderma; meanwhile, the waste bagasse is recycled, so that resources are fully utilized, and waste materials are changed into valuable materials.

The above is an embodiment of the present invention. The embodiments and specific parameters in the embodiments are only for the purpose of clearly illustrating the process of verifying the invention and are not intended to limit the scope of the invention, which is defined by the claims, and all the equivalent structural changes made by applying the content of the specification of the invention should be covered by the scope of the invention.

Claims (2)

1. An ecological high-efficiency planting method of subtropical ganoderma lucidum is characterized by comprising the following steps:

s1, weighing 10-20 parts of fresh green roughhaired holly leaves, 10-20 parts of fresh gelia japonica leaves, 20-25 parts of peptone, 30-40 parts of sugarcane juice, 20-25 parts of agar and 800 parts of water according to parts by weight, uniformly mixing the materials, and sterilizing to obtain a first-class seed culture medium; selecting a wild ganoderma variety grown in subtropical regions, inoculating a small piece of ganoderma flesh tissue on a primary culture medium by adopting a tissue isolation culture technology, and culturing for 7-10 days to obtain a primary seed;

s2, weighing 60 parts of miscellaneous wood chips, 40 parts of bagasse and 1.5 parts of gypsum powder according to parts by weight, uniformly mixing the materials, sterilizing to obtain a secondary seed culture medium, and inoculating the primary seed on the secondary seed culture medium to culture for 20-25 days to obtain a secondary seed;

s3, weighing 78 parts of the bakken wood chips, 10 parts of wheat bran, 1 part of sugar and 1 part of gypsum powder according to the parts by weight, uniformly mixing the materials, sterilizing to obtain a third-class seed culture medium, and inoculating the second-class seeds on the third-class seed culture medium to culture for 18-20 days to obtain third-class seeds;

s4, weighing 60 parts of the total of the green roughhaired holly, the mulberry twigs and the wood shavings, 38 parts of bagasse and 2 parts of gypsum powder according to the parts by weight, uniformly mixing the materials, sterilizing to obtain a ground culture compost, and then inoculating the third-level seeds on the ground culture compost to culture the lucid ganoderma.

2. The ecological high-efficiency subtropical ganoderma lucidum planting method according to claim 1, wherein the ecological high-efficiency subtropical ganoderma lucidum planting method comprises the following steps: the mixed wood dust comprises 50% of green-house wood dust and 50% of mulberry branch dust.