CN112514734A - Indoor cultivation method for phellinus igniarius - Google Patents
Indoor cultivation method for phellinus igniarius Download PDFInfo
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- CN112514734A CN112514734A CN202011197166.9A CN202011197166A CN112514734A CN 112514734 A CN112514734 A CN 112514734A CN 202011197166 A CN202011197166 A CN 202011197166A CN 112514734 A CN112514734 A CN 112514734A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
Abstract
The invention belongs to the technical field of edible and medicinal fungi production, and particularly relates to a phellinus igniarius fungus indoor cultivation method which comprises the steps of cultivation material preparation, bottling, sterilization, inoculation and spawn running, fruiting management and harvesting. The cultivation material is prepared by mixing 45-55% of mulberry sawdust, 35-25% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate. The bottling step is that the cultivation material is subpackaged into tissue culture bottles, and the filling amount is about 1cm away from the bottle mouth; the bottle mouth of the tissue culture bottle is sealed by a special bottle cap, and 1-2 fruiting openings with the width of 2mm and the length of 3cm are reserved on the bottle cap. According to the phellinus igniarius cultivation method provided by the invention, the growth of phellinus igniarius hyphae is fast, the cultivation period is short, and the primordium can occur 32 days after inoculation, which is about 13 days earlier than the fungus bag cultivation; the fruiting body is single, and the biotransformation rate is high and can reach about 36.4%; and the fruiting mode is simple, the method is suitable for large-scale production and is worth popularizing.
Description
Technical Field
The invention belongs to the technical field of edible fungus production, and particularly relates to an indoor cultivation method of phellinus igniarius.
Background
Phellinus linteus (Phellinus) belonging to the order Polyporales, the family Polyporaceae, the class Basidiomycetes, genus Phellinus. The fungus usually grows on Morus (Morus L) plants in the Central and south China, and the fruiting body of the fungus is yellow brown, so the fungus is named as Phellinus. It is a precious medicinal fungus developed in recent years and one of the 23 medicinal fungi with the most development prospect in China. Phellinus linteus fruiting body is slightly bitter in taste, and can be used for treating blood stranguria, rectocele, hemorrhage, leukorrhagia, amenorrhea, spleen deficiency and diarrhea. Relevant researches show that the water extract of phellinus igniarius has an inhibition rate of 96.7 percent on the S-180 of mice and an inhibition rate of 87 percent on ehrlich ascites carcinoma; polysaccharides in Phellinus linteus are the main components of anticancer effect. The research on phellinus igniarius has become a hot spot in the field of international medicinal fungi at present because phellinus igniarius has a remarkable anticancer effect. Traditionally, the active ingredient polysaccharide in phellinus linteus is usually extracted from fruit body; however, due to the increasing shortage of wild phellinus igniarius resources, and the unstable properties of artificially cultured phellinus igniarius strains, the further development and utilization of phellinus igniarius are limited.
Phellinus linteus is in various varieties, and research on Phellinus linteus mainly focuses on Phellinus linteus (Berk. et. Curt) Teng, Phellinus igniarius (L.et. Fr) Quel and Phellinus baumii (Phellinus baumii Pilat) at home and abroad at present. The phellinus igniarius singhuang Sheng h.wu, t.hatt & y.c.dai, a new species in the world discovered in 2012 in our country, only parasitize on mulberry trees, the fruiting bodies mostly cover and overlap together, are horseshoe-shaped or irregular, are lemon yellow to golden yellow in young, and are earthy yellow or yellowish brown in old; since Phellinus linteus (Inonotus sanghuanghuang) was found later, cultivation studies on the Phellinus linteus strain were less.
Chinese patent application No. CN201310252255.2 discloses a method for artificially cultivating phellinus igniarius; activating Phellinus Linteus strain, and taking 0.3cm under aseptic condition2Inoculating the strain blocks to a phellinus igniarius culture medium in the culture bags, and culturing at the constant temperature of 28 ℃ in a dark place until hypha grows over the culture bags to obtain culture strains; when the hypha in the cultivation bag begins to appear yellow or have a protruded tumor-shaped primordium, the fungus bag is moved to a yellow field, half bag removal and soil covering are carried out to obtain yellow cultivationAnd (5) nourishing.
Chinese patent with application number CN201510541900.1 discloses a method for cultivating phellinus igniarius, which comprises cutting ramulus mori into small wood segments, adding mulberry sawdust, wheat bran and gypsum, stirring uniformly, bagging, sterilizing, inoculating phellinus igniarius strain, and culturing for 25-35 days to obtain activated phellinus igniarius strain; preparing a phellinus igniarius cultivation medium by using mulberry sawdust, wheat bran, corn flour, monopotassium phosphate, magnesium sulfate, gypsum and white sugar, bagging, sterilizing and inoculating activated phellinus igniarius strains; then placing the mixture into a dark and ventilated culture room with the relative air humidity of 60-75% and the room temperature of 22-28 ℃ for culture; culturing for 60-80 days to obtain the phellinus igniarius.
The above patent adopts cultivation material containing mulberry wood dust to cultivate phellinus igniarius to simulate the growth process of phellinus igniarius in nature; but the method aims at the common phellinus igniarius strain, and has poor effect on the cultivation of the phellinus igniarius sanghuang Sheng h.wu, t.hatt & y.c.dai; and the traditional fungus bag cultivation mode is adopted, so that the problems of low phellinus igniarius biotransformation rate and long maturation time exist.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides an indoor cultivation method of phellinus linteus, in particular to a cultivation method of phellinus linteus sanghuang Sheng h.wu, t.hatt & y.c.dai, which is realized by the following technical scheme:
a phellinus igniarius indoor cultivation method comprises the following steps:
(1) preparing a cultivation material: mixing mulberry sawdust, cottonseed hulls, wheat bran, lime, gypsum, magnesium sulfate and potassium dihydrogen phosphate to prepare a cultivation material;
(2) bottling: subpackaging into tissue culture bottle, feeding material about 1cm away from the bottle mouth, sealing with film, and sterilizing;
(3) inoculation and spawn running: cooling the tissue culture bottle, inoculating the cultured strain, inoculating, placing in an indoor environment at 26 deg.C, and culturing for 10-15 days;
(4) and (3) fruiting management: after spawn running is finished, removing the sealing film, opening the bottle cap, removing old spawns, keeping air humidity and surface humidity of the base material by spraying water, covering the bottle cap when hyphae begin to kink after 3-4 days, and waiting for fruiting; after primordia occur, increasing humidity and ventilating to promote differentiation and growth of sporocarp;
(5) harvesting: and (4) starting harvesting 14-16 days after primordia occur when the basal part of the sporocarp turns brown from golden yellow, and finishing fruiting in a tissue culture bottle after harvesting.
Preferably, the cultivation material comprises the following raw materials in percentage by weight: 45-55% of mulberry sawdust, 35-25% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate; the pH is natural.
More preferably, the cultivation material comprises the following raw materials in percentage by weight: 50% of mulberry sawdust, 30% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate; the pH is natural.
Preferably, the tissue culture bottle has the specification of 600-.
Preferably, in the step (2), the inoculation amount of the cultivated species is 1-3% of the cultivation material quality.
Preferably, in the step (2), the bottle mouth of the tissue culture bottle is sealed by a special bottle cap, and 1-2 fruiting openings with the width of 2mm and the length of about 3cm are reserved on the bottle cap.
Preferably, in the step (4), during fruiting, the indoor air humidity is kept at 70-80%, and the surface humidity of the base material is kept at 60-65%.
Preferably, the mulberry sawdust is prepared by crushing mulberry wastes, sieving the crushed mulberry wastes with a 13mm sieve and air-drying the crushed mulberry wastes.
The sterilization is to sterilize the tissue culture bottle filled with the cultivation material for 120min at 121 ℃ and 0.1 Mpa.
The above cultivar is a phellinus linteus (i.sanghuang) cultivar obtained by culturing an Inonotus sanghuang Sheng h.wu, t.hatt & y.c.dai strain isolated from a phellinus linteus fruiting body parasitized on a wild mulberry.
The invention also researches the influence of different cultivation materials and cultivation modes on the growth of phellinus igniarius:
1. influence of different cultivation materials on the growth of Phellinus Linteus
1.1 test materials:
cultivating species: inonotus sanghuang Sheng h.wu, t.hatt & y.c.dai phellinus igniarius cultivars.
Raw materials of the cultivation material: the mulberry waste and cottonseed hulls are used as main materials, the wheat bran is used as an auxiliary material, and lime, gypsum, magnesium sulfate and potassium dihydrogen phosphate are used as additives. Wherein the mulberry waste, mulberry twigs and rejected mulberry wood are crushed, screened by a 13mm sieve and air-dried for standby; wheat bran, lime, gypsum, magnesium sulfate, etc. are purchased from local sites and used directly.
1.2 cultivation ingredient formula design:
a fungus bag fruiting method is adopted, the formula of the culture material for fungus bag cultivation fruiting is optimized and screened, 5 formulas are designed, and a conventional planting formula is used as a control group experiment, as shown in table 1.
Table 1 cultivation material formulation design (mass percent,%)
1.3 inoculation and culture: preparing a base material according to the formula of 1.2, filling the base material into 15 x 30 dog-ear fungus bags, sterilizing and inoculating phellinus igniarius cultivated species. Observing germination time, cover time, full growth time, fruiting time, maturation time and other indexes of the fungus bags, as well as yield per unit, biotransformation rate and other indexes, and performing data analysis by using SPSS 20.0 software, wherein the growth conditions of the phellinus igniarius in different cultivation materials are shown in table 2 and figure 1, and the agricultural properties and the biotransformation rate of the phellinus igniarius in non-cultivation materials are shown in table 3, figure 2 and figure 3.
TABLE 2 Phellinus Linteus hypha growth and fruiting body development in non-cultivated material
TABLE 3 agricultural traits and bioconversion rates of Phellinus linteus in different cultivation materials
As is clear from Table 2, Table 3 and FIGS. 1-2, the culture medium containing cottonseed hulls showed good growth of mycelia, and the content of cottonseed hulls was preferably 25 to 35%. When the mulberry sawdust is used as a culture medium, phellinus igniarius hyphae are light yellow and are easy to differentiate to form primordia, and the formed fruit bodies are darker in color. The optimal formula for obtaining the phellinus igniarius bag material cultivation is as follows: 50% of mulberry sawdust, 30% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate, 0.3% of potassium dihydrogen phosphate and natural pH. The biotransformation rate (freshness) of the fungus bag cultivation reaches about 20%. Primordia begin to form about 45 days after inoculation, shortening the cultivation period.
2. Influence of different cultivation modes on the growth of Phellinus igniarius
2.1 test materials:
cultivating species: inonotus sanghuang Sheng h.wu, t.hatt & y.c.dai phellinus igniarius cultivars.
Raw materials of the cultivation material: the mulberry waste and cottonseed hulls are used as main materials, the wheat bran is used as an auxiliary material, and lime, gypsum, magnesium sulfate and potassium dihydrogen phosphate are used as additives. Wherein the mulberry waste is mulberry twigs and rejected mulberry wood, and is crushed, sieved by a 13mm sieve and air-dried for standby; wheat bran, lime, gypsum, magnesium sulfate, etc. are purchased from local sites and used directly.
2.2 formula of cultivation material:
50% of mulberry sawdust, 30% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate; the pH is natural.
1.3 design of cultivation mode:
an indoor tissue culture bottle fruiting mode, a fungus bag fruiting mode and 4 outdoor fungus bag half-earthing and fungus bag-removing earthing cultivation modes are designed, influences of different cultivation modes on phellinus igniarius cultivation are explored through primordium generation, fruiting body generation, agricultural properties and the like, and 3 repetitions are set. The effect of different cultivation modes on phellinus linteus fruiting is shown in the following table 4 and fig. 3.
TABLE 4 influence of different cultivation modes on Phellinus linteus fruiting
As can be seen from Table 4 and FIG. 3, Phellinus linteus is difficult to fruiting outside and can normally fruiting indoors. When the indoor fruiting of phellinus igniarius, the fruiting conditions can be considered to be controlled, a lot of plant diseases and insect pests can be avoided, the fruiting can be effectively carried out, the outdoor fruiting takes the natural environment as the fruiting conditions, a lot of variable factors exist, the plant diseases and insect pests in the environment are more, and the fruiting is usually finished due to the fact that sundry fungi appear in the planting process. The fruiting of the tissue culture bottle culture can lead the hypha to grow rapidly and turn color, the edge of the bottle mouth is provided with a fruiting port, and the hypha grows rapidly towards the fruiting port after growing to full and forms primordium. The conversion rate of fresh mushroom reaches 36.4%.
The invention also researches the size of the fruiting port of the bottle cap of the tissue culture bottle, and finds that the primordium can occur most quickly when the width of the fruiting port is 2mm and the length is about 3cm, and the primordium can occur 32 days after inoculation.
The invention has the beneficial effects that:
1. the phellinus igniarius cultivation material provided by the invention is balanced in nutrient content, and the absorptivity of phellinus igniarius cultivated species is high; the biotransformation rate (freshness) of the fungus bag cultivation reaches about 20%, primordium begins to form about 45 days after inoculation, and the cultivation period is shortened.
2. The method adopts an indoor tissue culture bottle culture mode to culture the phellinus igniarius, can enable hyphae to grow rapidly and turn color, the edge of the mouth of the tissue culture bottle is provided with a fruiting port, and the hyphae grow towards the fruiting port rapidly after growing over, and form primordium, so that the yield is improved.
3. The phellinus igniarius cultivation method provided by the invention can realize annual production through indoor humidity control and temperature control. The phellinus igniarius hyphae grow fast, the cultivation period is short, the primordium can occur 32 days after inoculation, the time is about 13 days earlier than that of fungus bag cultivation, and the phellinus igniarius hyphae can be harvested about 46 days after inoculation; the fruiting body is single, and the biotransformation rate is high and can reach about 36.4%; and the fruiting mode is simple, the method is suitable for large-scale production and is worth popularizing.
Detailed Description
FIG. 1 is a bar graph showing the bioconversion rate of Phellinus linteus cultivars in different cultivars.
FIG. 2 shows the growth of Phellinus linteus cultivars 12d after inoculation in different media.
FIG. 3 shows the fruiting status of different cultivation modes.
Detailed Description
The technical solution of the present invention is further defined below with reference to the specific embodiments, but the scope of the claims is not limited to the description.
Example 1
A phellinus igniarius indoor cultivation method comprises the following steps:
(1) preparing a cultivation material: mixing 50% of mulberry sawdust, 30% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate to prepare a cultivation material, wherein the pH value is natural;
(2) bottling: subpackaging the cultivation material into tissue culture bottles with a loading distance of about 1cm, sealing the bottle mouth with film, and sterilizing at 121 deg.C and 0.1Mpa for 120 min;
(3) inoculation and spawn running: cooling the tissue culture bottle, inoculating phellinus igniarius (I.sanghuang) cultivars, and inoculating according to 1% of the mass of the cultivars; after inoculation, placing the mixture in an indoor environment at 26 ℃ for spawn running and culturing for 15 days;
(4) and (3) fruiting management: after spawn running is finished, removing the sealing film, opening the bottle cap, removing old spawns, keeping air humidity at 70-80% and surface humidity of the base material at 60-65% by spraying water, covering the bottle cap when hypha starts to kink after 3 days, and waiting for spawn running; primordia occur 32 days after inoculation, and after the primordia occur, the humidity is increased and ventilation is performed to promote the differentiation and growth of sporocarp;
(5) harvesting: and (4) after primordia occur for 14 days, the basal part of the sporocarp turns brown from golden yellow, harvesting is started, and the fruiting of the tissue culture bottle is finished after the harvesting is finished.
The mulberry sawdust is prepared by crushing mulberry wastes, sieving the crushed mulberry wastes with a 13mm sieve and air-drying the crushed mulberry wastes.
The tissue culture bottle is a wide-mouth bottle with the volume of 600 and 1000 ml.
And (2) sealing the bottle mouth of the tissue culture bottle by using a special bottle cap, and reserving 1-2 fruiting openings with the width of 2mm and the length of 3cm on the bottle cap.
In the above examples, the cultivated species of phellinus linteus (i.sanghuang) was harvested 46 days after inoculation, the biotransformation rate was 36.4%, and the fruiting body weight was 50-100g (fresh weight).
Example 2
A phellinus igniarius indoor cultivation method comprises the following steps:
(1) preparing a cultivation material: mixing 45% of mulberry sawdust, 35% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate to prepare a cultivation material, wherein the pH value is natural;
(2) bottling: subpackaging the cultivation material into tissue culture bottles with a loading distance of about 1cm, sealing the bottle mouth with film, and sterilizing at 121 deg.C and 0.1Mpa for 120 min;
(3) inoculation and spawn running: cooling the tissue culture bottle, inoculating phellinus igniarius (I.sanghuang) cultivars, and inoculating according to 1% of the mass of the cultivars; after inoculation, placing the mixture in an indoor environment at 26 ℃ for spawn running and culturing for 15 days;
(4) and (3) fruiting management: after spawn running is finished, removing the sealing film, opening the bottle cap, removing old spawns, keeping air humidity at 70-80% and surface humidity of the base material at 60-65% by spraying water, covering the bottle cap when hypha starts to kink after 4 days, and waiting for spawn running; primordia occur 31 days after inoculation, and after the primordia occur, the humidity is increased and ventilation is performed to promote the differentiation and growth of the sporocarp;
(5) harvesting: and (4) after the primordia occur for 16 days, the basal part of the sporocarp turns brown from golden yellow, harvesting is started, and the fruiting of the tissue culture bottle is finished after the harvesting is finished.
Preferably, the cultivation material comprises the following raw materials in percentage by weight: 50% of mulberry sawdust, 30% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate; the pH is natural.
The mulberry sawdust is prepared by crushing mulberry wastes, sieving the crushed mulberry wastes with a 13mm sieve and air-drying the crushed mulberry wastes.
The tissue culture bottle is a wide-mouth bottle with the volume of 600 and 1000 ml.
And (2) sealing the bottle mouth of the tissue culture bottle by using a special bottle cap, and reserving 1-2 fruiting openings with the width of 2mm and the length of 3cm on the bottle cap.
In the above examples, Phellinus linteus (I.sanghuanghuang) cultivars can be harvested after inoculation for 47 days, with a biotransformation rate of 36.3% and fruit body weight of 60-70g fresh products.
Example 3
A phellinus igniarius indoor cultivation method comprises the following steps:
(1) preparing a cultivation material: mixing 55% of mulberry sawdust, 25% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate to prepare a cultivation material, wherein the pH value is natural;
(2) bottling: subpackaging the cultivation material into tissue culture bottles with a loading distance of about 1cm, sealing the bottle mouth with film, and sterilizing at 121 deg.C and 0.1Mpa for 120 min;
(3) inoculation and spawn running: cooling the tissue culture bottle, inoculating phellinus igniarius (I.sanghuang) cultivars, and inoculating according to 1% of the mass of the cultivars; after inoculation, placing the mixture in an indoor environment at 26 ℃ for spawn running and culturing for 12 days;
(4) and (3) fruiting management: after spawn running is finished, removing the sealing film, opening the bottle cap, removing old spawns, keeping air humidity at 70-80% and surface humidity of the base material at 60-65% by spraying water, covering the bottle cap when hypha starts to kink after 3 days, and waiting for spawn running; primordia occur 32 days after inoculation, and after the primordia occur, the humidity is increased and ventilation is performed to promote the differentiation and growth of sporocarp;
(5) harvesting: and (5) 15 days after the primordium occurs, the base part of the sporocarp turns brown from golden yellow, harvesting is started, and the fruiting of the tissue culture bottle is finished after the harvesting is finished.
The mulberry sawdust is prepared by crushing mulberry wastes, sieving the crushed mulberry wastes with a 13mm sieve and air-drying the crushed mulberry wastes.
The tissue culture bottle is a wide-mouth bottle with the volume of 600 and 1000 ml.
And (2) sealing the bottle mouth of the tissue culture bottle by using a special bottle cap, and reserving 1-2 fruiting openings with the width of 2mm and the length of 3cm on the bottle cap.
In the above examples, Phellinus linteus (I.sanghuanghuang) cultivars were harvested after inoculation for 47 days, with a biotransformation rate of 36.6% and fruit body weight of 60-80g (fresh).
It should be noted that the above examples and test examples are only for further illustration and understanding of the technical solutions of the present invention, and are not to be construed as further limitations of the technical solutions of the present invention, and the invention which does not highlight essential features and significant advances made by those skilled in the art still belongs to the protection scope of the present invention.
Claims (9)
1. A phellinus igniarius indoor cultivation method is characterized by comprising the following steps:
(1) preparing a cultivation material: mixing mulberry sawdust, cottonseed hulls, wheat bran, lime, gypsum, magnesium sulfate and potassium dihydrogen phosphate to prepare a cultivation material;
(2) bottling: subpackaging the cultivation material in the step (1) into tissue culture bottles, wherein the loading amount is about 1cm away from the bottle mouth, then sealing the bottle mouth, sealing with a film, and sterilizing;
(3) inoculation and spawn running: cooling the tissue culture bottle, inoculating the cultured strain, inoculating, placing in an indoor environment at 26 deg.C, and culturing for 10-15 days;
(4) and (3) fruiting management: after spawn running is finished, removing the sealing film, opening the bottle cap, removing old spawns, keeping air humidity and surface humidity of the base material by spraying water, covering the bottle cap when hyphae begin to kink after 3-4 days, and waiting for fruiting; after the primordium is exported, increasing the humidity and ventilating to promote the differentiation and growth of the sporocarp;
(5) harvesting: and (4) starting harvesting 14-16 days after primordia occur when the basal part of the sporocarp turns brown from golden yellow, and finishing fruiting in a tissue culture bottle after harvesting.
2. The indoor phellinus igniarius cultivation method according to claim 1, wherein the cultivation material comprises the following raw materials in percentage by weight: 45-55% of mulberry sawdust, 35-25% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate; the pH is natural.
3. The indoor phellinus igniarius cultivation method according to claim 1, wherein the cultivation material comprises the following raw materials in percentage by weight: 50% of mulberry sawdust, 30% of cottonseed hulls, 17.5% of wheat bran, 1% of lime, 1% of gypsum, 0.2% of magnesium sulfate and 0.3% of monopotassium phosphate.
4. The indoor phellinus igniarius cultivation method of claim 1, wherein the tissue culture bottle is a 600ml-1000ml wide-mouth bottle.
5. The indoor Phellinus linteus cultivation method of claim 1, wherein in step (2), the inoculum size of the cultivated species is 1% -3% of the cultivation material quality.
6. The indoor phellinus igniarius cultivation method according to claim 1, wherein in the step (2), the mouth of the tissue culture bottle is sealed by a special bottle cap, and 1-2 fruiting openings with the width of 2mm and the length of about 3cm are reserved on the bottle cap.
7. The method for indoor cultivation of Phellinus linteus according to claim 1, wherein in step (4), during fruiting, the indoor air humidity is maintained at 70-80% and the surface humidity of the base material is maintained at 60-65%.
8. The method for indoor cultivation of Phellinus linteus according to claim 1, wherein the mulberry wood flour is prepared by pulverizing mulberry waste, sieving with 13mm sieve, and air drying.
9. The method for indoor cultivation of Phellinus linteus according to any one of claims 1 to 8, wherein Phellinus linteus is Phellinus linteus Inonotus sanghuang Sheng H.Wu, T.hatt & Y.C.Dai.
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| CN113796261A (en) * | 2021-09-30 | 2021-12-17 | 安太成 | Artificial cultivation method of phellinus igniarius with short growth cycle |
| CN114451216A (en) * | 2022-02-25 | 2022-05-10 | 内蒙古科学技术研究院 | Large-scale artificial cultivation method for phellinus igniarius sporocarp |
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