CN114451216B - Large-scale artificial cultivation method for Phellinus linteus fruiting bodies - Google Patents
Large-scale artificial cultivation method for Phellinus linteus fruiting bodies Download PDFInfo
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- CN114451216B CN114451216B CN202210179375.3A CN202210179375A CN114451216B CN 114451216 B CN114451216 B CN 114451216B CN 202210179375 A CN202210179375 A CN 202210179375A CN 114451216 B CN114451216 B CN 114451216B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
According to the invention, a hollow cylindrical plastic pipe fitting is selected, the liquid culture medium is added into the hollow part, and the solid culture medium is filled at the two ends of the plastic pipe fitting to prepare the fungus sticks, so that Phellinus linteus fruiting body is cultivated, the defects of wood waste, long growth period, insufficient nutrition, easiness in infection and high cost of the liquid culture medium are avoided, the advantages of the solid culture medium and the liquid culture medium are integrated, the fungus sticks are simple to manufacture and low in cost, the probability of scratching fungus bags can be reduced, pollution is avoided, and a good foundation is provided for the standardized cultivation process of Phellinus linteus. The technical process of the invention can realize the control of standardized quality and technical parameters, and can realize the mode of artificial cultivation of mulberry Huang Jixie in standardized, industrialized, large-scale and standardized mode.
Description
Technical Field
The invention belongs to the technical field of artificial cultivation of phellinus linteus, and particularly relates to a large-scale artificial cultivation method of phellinus linteus fruiting bodies.
Background
Phellinus linteus is a medicinal fungus belonging to basidiomycotina subphylum and also called Phellinus baumii, and the fruiting body of Phellinus baumii has multiple functions of resisting tumor, resisting oxidation and the like, so that the Phellinus linteus becomes a research hotspot at home and abroad, and is the fungus with highest biological anticancer efficiency internationally recognized.
In recent years, the increase of the market demand for Phellinus linteus causes that the predatory exploitation of wild Phellinus linteus cannot be stopped, and the fruit body formed in nature is very rare due to the limitation of the physiological and ecological specificity, the complexity and the external conditions, the wild natural mulberry Huang Ziyuan is deficient, the deficiency is faced, the artificial domestication and cultivation difficulty of Phellinus linteus is higher, the stable medical industry product source is difficult to form, and the development of Phellinus linteus industry is greatly limited.
At present, two modes of artificial cultivation are mainly adopted, one mode is that the cultivation of the log is mainly carried out on the mulberry, the poplar and the like, a large amount of wood resources are needed for the cultivation of the log, the large-scale production is difficult, the other mode is bag material cultivation, the culture medium for the bag material cultivation has the problems of insufficient nutrition, small fruiting body development and easiness in infection of mixed bacteria, and the solid culture medium is adopted to solve the problems of uneven strain dispersion and long growth period. Meanwhile, the liquid culture medium is adopted for artificial cultivation, so that the method has the advantages of convenience in inoculation, short cultivation period, low pollution rate, controllable inoculation amount, consistent quantification and rapid field planting, but the technical problems of single formula and high cost of the liquid culture medium still exist, and the large-scale application of the liquid culture medium is limited.
Therefore, how to select a proper culture medium and a proper culture method to shorten the artificial cultivation period of Phellinus linteus, reduce the production cost, and adapt to large-scale production is still a difficult point to be solved by the technicians in the field.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the large-scale artificial cultivation method for the Phellinus linteus fruiting bodies, which realizes a short Phellinus linteus fruiting body growth period, reduces the production cost and can meet the requirement of large-scale production by adjusting the composition and the proportion of a culture medium and optimizing the technological parameters in the cultivation method.
In order to achieve the above purpose, the invention adopts the following technical scheme that the large-scale artificial cultivation method of Phellinus linteus fruiting bodies comprises the following steps:
(1) Preparation of solid Medium
Weighing 20-40 parts of cotton seed hulls, 30-60 parts of mulberry wood chips, 5-10 parts of bran, 1-3 parts of glucose, 2-4 parts of sucrose, 1-2 parts of hydroxyapatite, 2-6 parts of monopotassium phosphate, 1-3 parts of calcium sulfate and 3-6 parts of sodium alginate, uniformly mixing, and controlling the water content to be 60-70% to obtain a solid culture medium;
(2) Preparation of liquid culture Medium
Taking water as a solvent, and each liter of culture medium comprises the following raw materials by weight: 10-20g of corn flour, 2-5g of monopotassium phosphate, 1-3g of magnesium sulfate, 10-15g of glucose, 0.002-0.005g of vitamin B, 5-8g of peptone and 0.5-1g of magnesium acetate tetrahydrate; uniformly mixing all the components with water to obtain a liquid culture medium;
(3) Preparation of fungus stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture medium into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, fastening the two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) Inoculation and fermentation
Inoculating Phellinus linteus strain on solid culture medium at two ends of fungus stick, performing dark culture at 25-30deg.C and humidity of 60-70%, and ending fermentation when two thirds of fungus bag is full of strain;
(5) Culturing in yellow
Transferring the fungus sticks after the fungus growth is finished into a greenhouse, performing culture at 25-30 ℃ and humidity of 80-90% under V-shaped notch openings, ventilating for 30-40min at intervals of 6h each day, controlling CO2 concentration at 2000-2500ppm, controlling illumination at 200-300lux, and culturing for 15-20 days;
adjusting the temperature to 25-30deg.C, humidity to above 95%, CO2 concentration below 1500ppm, illumination to 200-300lux, ventilation for 30-40min every 4 hr, and culturing until fruiting body is mature;
(6) Drying
The harvested Phellinus linteus fruiting bodies are dried in a constant temperature drying oven at 50-60 ℃ for 10-12h, so that the water content of the Phellinus linteus fruiting bodies is below 1.3%.
The cotton seed hulls are prepared by crushing the cotton seed hulls through a 200-300 mesh screen, the particle size of the mulberry sawdust is 0.5-2cm, and the particle size of the hydroxyapatite is 80-120nm; the grain size of the corn flour is 100-200 meshes;
the hollow cylindrical plastic pipe fitting has the length of 20-35cm and the diameter of 10-15cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition board; sterilizing in the step (3) under the pressure of 0.2-0.5MPa and the temperature of 130-140 ℃ for 10-20min;
in the yellow culture in the step (5), a sunshade net is arranged on the greenhouse, and one of green and white films or white films is added on the sunshade net.
In the yellow culture in the step (5), the plastic partition plate is taken out, and the fungus sticks are periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
Compared with the prior art, the invention has the following beneficial effects:
(1) According to the invention, a hollow cylindrical plastic pipe fitting is selected, the liquid culture medium is added into the hollow part, and the solid culture medium is filled at the two ends of the plastic pipe fitting to prepare the fungus sticks, so that Phellinus linteus fruiting body is cultivated, the defects of wood waste, long growth period, insufficient nutrition, easiness in infection and high cost of the liquid culture medium are avoided, the advantages of the solid culture medium and the liquid culture medium are integrated, the fungus sticks are simple to manufacture and low in cost, the probability of scratching fungus bags can be reduced, pollution is avoided, and a good foundation is provided for the standardized cultivation process of Phellinus linteus.
(2) The invention optimizes the composition and the proportion of the solid culture medium and the liquid culture medium, reduces the consumption of mulberry sawdust, increases the consumption of cotton seed hulls, reduces the need of wood resources, and simultaneously, the culture medium can contain nutrient substances needed or needed to be enriched by the Phellinus linteus, thereby being beneficial to the rapid extension of Sang Huangsi body, shortening the culture period, and scientifically, repeatedly and stably researching and realizing the improvement of the quality of Phellinus linteus fruiting bodies.
(3) The technical process of the invention can realize the control of standardized quality and technical parameters, and can realize the mode of artificial cultivation of mulberry Huang Jixie in standardized, industrialized, large-scale and standardized mode.
Detailed Description
For a further understanding of the present invention, preferred embodiments of the invention are described below in conjunction with the examples, but it should be understood that these descriptions are merely intended to illustrate further features and advantages of the invention, and are not limiting of the claims of the invention. All the raw materials of the present invention are not particularly limited in their sources, and may be purchased on the market or prepared according to conventional methods well known to those skilled in the art.
Example 1
A large-scale artificial cultivation method of Phellinus linteus fruiting body comprises the following steps:
(1) Preparation of solid Medium
40 parts of cotton seed hulls, 30 parts of mulberry wood chips, 10 parts of bran, 3 parts of glucose, 2 parts of sucrose, 1 part of hydroxyapatite, 3 parts of monopotassium phosphate, 1 part of calcium sulfate and 6 parts of sodium alginate are weighed and uniformly mixed, and the water content is controlled to be 70%, so that a solid culture medium is obtained;
(2) Preparation of liquid culture Medium
Taking water as a solvent, and each liter of culture medium comprises the following raw materials by weight: 10g of corn flour, 5g of monopotassium phosphate, 3g of magnesium sulfate, 15g of glucose, 0.005g of vitamin B, 5g of peptone and 0.5g of magnesium acetate tetrahydrate; uniformly mixing all the components with water to obtain a liquid culture medium;
(3) Preparation of fungus stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture medium into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, fastening the two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) Inoculation and fermentation
Inoculating Phellinus linteus strain on solid culture medium at two ends of fungus stick, performing dark culture, controlling temperature to 25deg.C, culturing under humidity of 70%, and ending fermentation when two thirds of fungus bag is full of strain;
(5) Culturing in yellow
Transferring the fungus sticks after the fungus growth to a greenhouse, wherein the opening is a V-shaped notch, culturing at the temperature of 30 ℃ and the humidity of 80%, ventilating for 30min at intervals of 6 hours each day, controlling the CO2 concentration at 2000ppm, controlling the illumination at 200lux, and culturing for 15 days;
adjusting the temperature to 30deg.C, humidity to above 95%, CO2 concentration below 1500ppm, illumination to 300lux, ventilation for 40min every 4 hr, and culturing until fruiting body is mature;
(6) Drying
Drying the harvested Phellinus linteus fruiting body in a constant temperature drying oven at 60deg.C for 11 hr to make the water content of Phellinus linteus fruiting body reach below 1.3%.
The cotton seed hulls are prepared by crushing and sieving with a 200-mesh sieve, the particle size of the mulberry sawdust is 0.5cm, and the particle size of the hydroxyapatite is 120nm; the grain size of the corn flour is 200 meshes;
the hollow cylindrical plastic pipe fitting has the length of 35cm and the diameter of 15cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate; the sterilization in the step (3) is carried out for 10min under the pressure of 0.5MPa and the temperature of 140 ℃;
in the yellow culture in the step (5), a sunshade net is arranged on the greenhouse, and a green-white film is added on the sunshade net; in the yellow culture in the step (5), the plastic partition plate is taken out, and the fungus sticks are periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
Example 2
A large-scale artificial cultivation method of Phellinus linteus fruiting body comprises the following steps:
(1) Preparation of solid Medium
Weighing 20 parts of cotton seed hulls, 60 parts of mulberry wood chips, 10 parts of bran, 3 parts of glucose, 2 parts of sucrose, 1 part of hydroxyapatite, 2 parts of monopotassium phosphate, 1 part of calcium sulfate and 4 parts of sodium alginate, uniformly mixing, and controlling the water content to be 65% to obtain a solid culture medium;
(2) Preparation of liquid culture Medium
Taking water as a solvent, and each liter of culture medium comprises the following raw materials by weight: 15g of corn flour, 5g of monopotassium phosphate, 1g of magnesium sulfate, 12g of glucose, 0.004g of vitamin B, 8g of peptone and 1g of magnesium acetate tetrahydrate; uniformly mixing all the components with water to obtain a liquid culture medium;
(3) Preparation of fungus stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture medium into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, fastening the two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) Inoculation and fermentation
Inoculating Phellinus linteus strain on solid culture medium at two ends of fungus stick, performing dark culture at 28deg.C under humidity of 70%, and ending fermentation when two thirds of fungus bag is full of strain;
(5) Culturing in yellow
Transferring the fungus sticks after the fungus growth to a greenhouse, wherein the opening is a V-shaped notch, culturing at the temperature of 28 ℃ and the humidity of 85%, ventilating for 30min at intervals of 6 hours each day, controlling the concentration of CO2 at 2500ppm, controlling the illumination at 250lux, and culturing for 20 days;
adjusting the temperature to 30deg.C, humidity to above 95%, CO2 concentration below 1500ppm, illumination to 200lux, ventilation for 40min every 4 hr, and culturing until fruiting body is mature;
(6) Drying
Drying the harvested Phellinus linteus fruiting body in a constant temperature drying oven at 50deg.C for 12 hr to make the water content of Phellinus linteus fruiting body reach below 1.3%.
The cotton seed hulls are prepared by crushing and sieving with a 300-mesh sieve, the particle size of the mulberry sawdust is 2cm, and the particle size of the hydroxyapatite is 120nm; the grain size of the corn flour is 200 meshes;
the hollow cylindrical plastic pipe fitting has the length of 20cm and the diameter of 10cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate; the sterilization in the step (3) is carried out for 10min under the pressure of 0.5MPa and the temperature of 130 ℃; in the yellow culture of the step (5), a sunshade net is arranged on a greenhouse, a white film is added on the sunshade net, and in the yellow culture of the step (5), a plastic partition plate is taken out, fungus sticks are periodically shaken, and the liquid culture medium is promoted to permeate into the solid culture medium.
Example 3
A large-scale artificial cultivation method of Phellinus linteus fruiting body comprises the following steps:
(1) Preparation of solid Medium
30 parts of cotton seed hulls, 50 parts of mulberry chips, 6 parts of bran, 2 parts of glucose, 3 parts of sucrose, 1 part of hydroxyapatite, 5 parts of monopotassium phosphate, 3 parts of calcium sulfate and 6 parts of sodium alginate are weighed and uniformly mixed, and the water content is controlled to be 70%, so that a solid culture medium is obtained;
(2) Preparation of liquid culture Medium
Taking water as a solvent, and each liter of culture medium comprises the following raw materials by weight: 15g of corn flour, 3g of monopotassium phosphate, 3g of magnesium sulfate, 10g of glucose, 0.005g of vitamin B, 8g of peptone and 0.5g of magnesium acetate tetrahydrate; uniformly mixing all the components with water to obtain a liquid culture medium;
(3) Preparation of fungus stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture medium into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, fastening the two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) Inoculation and fermentation
Inoculating Phellinus linteus strain on solid culture medium at two ends of fungus stick, performing dark culture, controlling temperature to 26 deg.C and humidity to 65%, and finishing fermentation when two thirds of fungus bags are full of strain;
(5) Culturing in yellow
Transferring the fungus sticks after the fungus growth to a greenhouse, performing culture at the temperature of 27 ℃ and the humidity of 85% with the opening being a V-shaped incision, ventilating for 35min at intervals of 6h each day, controlling the CO2 concentration at 2200ppm, controlling the illumination at 260lux, and culturing for 18 days;
adjusting the temperature to 30deg.C, humidity to above 95%, CO2 concentration below 1500ppm, illumination to 300lux, ventilation for 40min every 4 hr, and culturing until fruiting body is mature;
(6) Drying
The harvested Phellinus linteus fruiting bodies are dried in a constant temperature drying oven at 55 ℃ for 10 hours, so that the water content of the Phellinus linteus fruiting bodies is below 1.3%.
The cotton seed hulls are prepared by crushing and sieving with a 250-mesh sieve, the particle size of the mulberry sawdust is 1cm, and the particle size of the hydroxyapatite is 100nm; the grain size of the corn flour is 150 meshes;
the hollow cylindrical plastic pipe fitting has the length of 30cm and the diameter of 12cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate; the sterilization in the step (3) is carried out for 15min under the pressure of 0.3MPa and the temperature of 135 ℃;
in the yellow culture in the step (5), a sunshade net is arranged on the greenhouse, and a green-white film is added on the sunshade net; in the yellow culture in the step (5), the plastic partition plate is taken out, and the fungus sticks are periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
Example 4
A large-scale artificial cultivation method of Phellinus linteus fruiting body comprises the following steps:
(1) Preparation of solid Medium
Weighing 28 parts of cotton seed hulls, 42 parts of mulberry chips, 7 parts of bran, 2 parts of glucose, 4 parts of sucrose, 2 parts of hydroxyapatite, 6 parts of monopotassium phosphate, 3 parts of calcium sulfate and 4 parts of sodium alginate, uniformly mixing, and controlling the water content to be 60% to obtain a solid culture medium;
(2) Preparation of liquid culture Medium
Taking water as a solvent, and each liter of culture medium comprises the following raw materials by weight: 18g of corn flour, 5g of monopotassium phosphate, 3g of magnesium sulfate, 10g of glucose, 0.003g of vitamin B, 8g of peptone and 1g of magnesium acetate tetrahydrate; uniformly mixing all the components with water to obtain a liquid culture medium;
(3) Preparation of fungus stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture medium into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, fastening the two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) Inoculation and fermentation
Inoculating Phellinus linteus strain on solid culture medium at two ends of fungus stick, performing dark culture, controlling temperature to 30deg.C, and culturing under humidity of 70%, and ending fermentation when two thirds of fungus bag is full of strain;
(5) Culturing in yellow
Transferring the fungus sticks after the fungus growth to a greenhouse, performing culture at 258 ℃ and 80% humidity with ventilation for 30min at 6h intervals each day, controlling CO2 concentration at 2300ppm, controlling illumination at 240lux, and culturing for 17 days;
regulating temperature to 30deg.C, humidity to above 95%, CO2 concentration below 1500ppm, illumination to 230lux, ventilation for 40min every 4 hr, and culturing until fruiting body is mature;
(6) Drying
Drying the harvested Phellinus linteus fruiting body in a constant temperature drying oven at 60deg.C for 10 hr to make the water content of Phellinus linteus fruiting body reach below 1.3%.
The cotton seed hulls are prepared by crushing and sieving with a 200-mesh sieve, the particle size of the mulberry sawdust is 1.5cm, and the particle size of the hydroxyapatite is 90nm; the grain size of the corn flour is 180 meshes;
the hollow cylindrical plastic pipe fitting has the length of 32cm and the diameter of 13cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate; the sterilization in the step (3) is carried out for 20min at 140 ℃ under the pressure of 0.4 MPa;
in the yellow culture in the step (5), a sunshade net is arranged on the greenhouse, and a white film is added on the sunshade net; in the yellow culture in the step (5), the plastic partition plate is taken out, and the fungus sticks are periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
It will be readily appreciated by those skilled in the art that the foregoing description is merely a preferred embodiment of the invention and is not intended to limit the invention, but any modifications, equivalents, improvements or alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (7)
1. A large-scale artificial cultivation method of Phellinus linteus fruiting body is characterized in that: the method comprises the following steps:
(1) Preparation of solid Medium
Weighing 20-40 parts of cotton seed hulls, 30-60 parts of mulberry chips, 5-10 parts of bran, 1-3 parts of glucose, 2-4 parts of sucrose, 1-2 parts of hydroxyapatite, 2-6 parts of monopotassium phosphate, 1-3 parts of calcium sulfate and 3-6 parts of sodium alginate, uniformly mixing, and controlling the water content to be 60-70% to obtain a solid culture medium;
(2) Preparation of liquid culture Medium
Taking water as a solvent, and each liter of liquid culture medium comprises the following raw materials by weight: 10-20g of corn flour, 2-5g of monopotassium phosphate, 1-3g of magnesium sulfate, 10-15g of glucose, 0.002-0.005g of vitamin B, 5-8g of peptone and 0.5-1g of magnesium acetate tetrahydrate; uniformly mixing all the components with water to obtain a liquid culture medium;
(3) Preparation of fungus stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture medium into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, fastening the two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) Inoculation and fermentation
Inoculating Phellinus linteus strain on solid culture medium at two ends of fungus stick, performing dark culture at 25-30deg.C and humidity of 60-70%, and ending fermentation when two thirds of fungus stick is full of strain;
(5) Culturing in yellow
Will develop bacteriaTransferring the fungus sticks into a greenhouse, cutting the fungus sticks into V-shaped openings, culturing at 25-30deg.C and humidity of 80-90%, ventilating for 30-40min every day for 6 hr, and introducing CO 2 The concentration is controlled at 2000-2500ppm, the illumination is controlled at 200-300lux, after culturing for 15-20 days, the temperature is adjusted to 25-30 ℃, the humidity is more than 95%, and CO 2 The concentration is lower than 1500ppm, the illumination is controlled at 200-300lux, ventilation is carried out for 30-40min at intervals of 4h each day, and the culture is continued until the fruiting body is mature;
(6) Drying
Drying the harvested Phellinus linteus fruiting bodies in a constant temperature drying oven at 50-60 ℃ for 10-12h to ensure that the water content of the Phellinus linteus fruiting bodies is below 1.3%;
the hollow cylindrical plastic pipe fitting has the length of 20-35cm and the diameter of 10-15cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition board;
in the yellow culture in the step (5), the plastic partition plate is taken out, and the fungus sticks are periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
2. The large-scale artificial cultivation method of Phellinus linteus fruiting body of claim 1, wherein: the cotton seed hulls are prepared by crushing the cotton seed hulls and sieving the crushed cotton seed hulls with a 200-300-mesh sieve.
3. The large-scale artificial cultivation method of Phellinus linteus fruiting body of claim 1, wherein: the particle size of the mulberry wood dust is 0.5-2cm.
4. The large-scale artificial cultivation method of Phellinus linteus fruiting body of claim 1, wherein: the particle size of the hydroxyapatite is 80-120nm.
5. The large-scale artificial cultivation method of Phellinus linteus fruiting body of claim 1, wherein: the grain size of the corn flour is 100-200 meshes.
6. The large-scale artificial cultivation method of Phellinus linteus fruiting body of claim 1, wherein: the sterilization in the step (3) is carried out for 10-20min under the pressure of 0.2-0.5MPa and the temperature of 130-140 ℃.
7. The large-scale artificial cultivation method of Phellinus linteus fruiting body of claim 1, wherein: in the yellow culture in the step (5), a sunshade net is arranged on the greenhouse, and one of green and white films or white films is added on the sunshade net.
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| CN112322572A (en) * | 2020-11-19 | 2021-02-05 | 陕西省微生物研究所 | Liquid fermentation method for increasing yield of phellinus igniarius mycelium |
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