CN112322572A - Liquid fermentation method for increasing yield of phellinus igniarius mycelium - Google Patents
Liquid fermentation method for increasing yield of phellinus igniarius mycelium Download PDFInfo
- Publication number
- CN112322572A CN112322572A CN202011305933.3A CN202011305933A CN112322572A CN 112322572 A CN112322572 A CN 112322572A CN 202011305933 A CN202011305933 A CN 202011305933A CN 112322572 A CN112322572 A CN 112322572A
- Authority
- CN
- China
- Prior art keywords
- parts
- liquid
- phellinus igniarius
- culture medium
- yield
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a liquid fermentation method for improving the yield of phellinus igniarius mycelium, which comprises the following steps: s1: preparing a liquid culture medium for culturing phellinus igniarius; s2, inoculating phellinus igniarius strains into a liquid culture medium, performing activated culture, then inoculating the phellinus igniarius strains into a seed culture medium according to the inoculation amount of 10-15% of the volume percentage, and performing seed culture to obtain seed liquid; s3, inoculating the seed solution prepared in the step S2 into a liquid culture medium according to the inoculation amount of 10-15% in volume percentage, performing liquid fermentation culture for 1-3 days at the temperature of 28-35 ℃, adding a certain amount of vegetable oil and magnesium acetate tetrahydrate in any day of the culture process, and continuing to culture for 2-3 days at the temperature of 23-28 ℃ to obtain a mixed solution of phellinus igniarius mycelia and a fermentation liquid. The invention aims to provide a liquid fermentation method for improving the yield of phellinus igniarius mycelia, which effectively improves the yield.
Description
Technical Field
The invention relates to a liquid fermentation method for improving the yield of phellinus igniarius mycelium.
Background
Phellinus linteus is a perennial medicinal fungus and one of rare and endangered traditional Chinese medicines with the greatest development prospect in China. Mainly distributed in the region of the Qinling mountain, Shaanxi and Gansu junction, i.e. "midnight mountain". Growing on withered wood and trunk of broad-leaved tree such as poplar, mulberry, willow, white birch, zelkova, peach, etc. Recorded in the Chinese medicine dictionary, it can treat gynecological diseases such as metrorrhagia, stranguria with blood, leukorrhagia, amenorrhea, etc. Modern researches find that phellinus igniarius has obvious hepatic fibrosis resisting effect and is the first higher fungus with high efficiency in the field of biological cancer treatment internationally acknowledged at present.
At present, the phellinus igniarius products mostly depend on phellinus igniarius sporophores, the phellinus igniarius sporophores are highly dependent on the environment in growth, the growth period is long, and the yield is unstable.
The prior art shows that the liquid culture of phellinus igniarius has low yield generally.
Disclosure of Invention
The invention aims to provide a liquid fermentation method for improving the yield of phellinus igniarius mycelia, which effectively improves the yield.
In order to solve the technical problems, the invention adopts the following technical scheme:
the invention relates to a liquid fermentation method for improving the yield of phellinus igniarius mycelium, which comprises the following steps:
s1: preparing a liquid culture medium for culturing phellinus igniarius, wherein the liquid culture medium comprises the following components in parts by weight: 20-30 parts of maltose, 20-30 parts of yeast powder, 10-20 parts of Chinese yam, 10-20 parts of soybean powder, 10-20 parts of potato powder, 0.5-1 part of triammonium citrate, 0.001-0.005 part of vitamin B and the balance of water;
s2, inoculating phellinus igniarius strains into a liquid culture medium, performing activated culture, then inoculating the phellinus igniarius strains into a seed culture medium according to the inoculation amount of 10-15% of the volume percentage, and performing seed culture to obtain seed liquid;
s3, inoculating the seed solution prepared in the step S2 into a liquid culture medium according to the inoculation amount of 10-15% in volume percentage, performing liquid fermentation culture for 1-3 days at the temperature of 28-35 ℃, adding a certain amount of vegetable oil and magnesium acetate tetrahydrate in any day of the culture process, and continuing to culture for 2-3 days at the temperature of 23-28 ℃ to obtain a mixed solution of phellinus igniarius mycelia and a fermentation liquid;
and S4, performing pressure filtration on the mixed liquid of the phellinus igniarius mycelia and the fermentation liquid obtained in the step II through a plate-and-frame filter press, drying a filter cake at the temperature of 70-80 ℃, performing vacuum concentration on the filtrate, performing spray drying on the filtrate in a spray dryer to obtain dry powder, and mixing the phellinus igniarius mycelia and the dry powder to obtain the phellinus igniarius fermentation mycelia.
Further, the liquid culture medium comprises the following components in parts by weight: 20 parts of maltose, 20 parts of yeast powder, 10 parts of Chinese yam, 10 parts of soybean meal, 10 parts of potato powder, 0.5 part of triammonium citrate, 0.002 part of vitamin B and the balance of water.
Further, the liquid culture medium comprises the following components in parts by weight: 30 parts of maltose, 30 parts of yeast powder, 20 parts of Chinese yam, 20 parts of soybean meal, 20 parts of potato powder, 1 part of triammonium citrate, 0.005 part of vitamin B and the balance of water.
Further, the liquid culture medium comprises the following components in parts by weight: 25 parts of maltose, 25 parts of yeast powder, 15 parts of Chinese yam, 15 parts of soybean meal, 15 parts of potato powder, 0.8 part of triammonium citrate, 0.004 part of vitamin B and the balance of water.
Furthermore, the addition amount of the vegetable oil is 2-10 g/L.
Further, the addition amount of the magnesium acetate tetrahydrate is 0.5-1 g/L.
Furthermore, the addition amount of the vegetable oil is 5-8 g/L.
Further, the vegetable oil comprises one of soybean oil, olive oil or rapeseed oil.
Further, the culture conditions in S2 are: culturing at 25-28 deg.C for 3-5 days.
Further, the vacuum degree of vacuum concentration is 0.09-0.1 MPa, the inlet temperature of the spray drying process is 136-141 ℃, the outlet temperature is 81-82 ℃, the rotation speed of a peristaltic pump is 8r/min, the rotation speed of a fan is 906r/min, and the working pressure is 0.23-0.24 MPa.
Compared with the prior art, the invention has the beneficial technical effects that:
according to the invention, through reasonably configuring the culture medium suitable for the growth of the phellinus linteus fermentation mycelium, yeanxiang is matched with the addition of quantitative vegetable oil and tetrahydrate magnesium acetate in the culture process, so that the yield of the phellinus linteus fermentation mycelium is greatly improved.
Detailed Description
Example 1
A liquid fermentation method for increasing the yield of Phellinus linteus mycelium comprises:
s1: the liquid culture medium comprises the following components in parts by weight: 20g of maltose, 20g of yeast powder, 10g of Chinese yam, 10g of soybean meal, 10g of potato powder, 0.5g of triammonium citrate and 0.002g of vitamin B, and preparing into 200ml of liquid culture medium;
s2, inoculating phellinus igniarius strains into a liquid culture medium, performing activated culture, then inoculating the phellinus igniarius strains into a seed culture medium according to 12% of inoculation amount by volume percentage, and performing seed culture to obtain seed liquid;
s3, inoculating the seed solution prepared in the step S2 into a liquid culture medium according to the inoculation amount of 10-15% in volume percentage, performing liquid fermentation culture for 2 days at the temperature of 30 ℃, adding a certain amount of vegetable oil and magnesium acetate tetrahydrate in any day of the culture process, and continuing to culture for 3 days at the temperature of 25 ℃ to obtain a mixed solution of phellinus igniarius mycelia and fermentation liquor;
and S4, performing pressure filtration on the mixed liquid of the phellinus igniarius mycelia and the fermentation liquid obtained in the step II through a plate-and-frame filter press, then drying the filter cake at the temperature of 75 ℃, performing vacuum concentration on the filtrate, performing spray drying on the filtrate in a spray dryer to obtain dry powder, and mixing the phellinus igniarius mycelia and the dry powder to obtain the phellinus igniarius fermentation mycelia.
Wherein the vegetable oil is soybean oil and the addition amount is 6 g/L. The addition amount of the magnesium acetate tetrahydrate is 0.8 g/L. The culture conditions in S2 were: the temperature was 26 ℃ and shaking culture was carried out for 4 days. The vacuum degree of vacuum concentration is 0.09-0.1 MPa, the inlet temperature of the spray drying process is 136-141 ℃, the outlet temperature of the spray drying process is 81-82 ℃, the rotating speed of a peristaltic pump is 8r/min, the rotating speed of a fan is 906r/min, and the working pressure is 0.23-0.24 MPa.
Example 2
The difference from example 1 is: the liquid culture medium comprises the following components in parts by weight: 30g of maltose, 30g of yeast powder, 20g of yam, 20g of soybean meal, 20g of potato powder, 1g of triammonium citrate and 0.005g of vitamin B, and the preparation is 300-400ml of liquid culture medium.
Example 3
The difference from example 1 is: the liquid culture medium comprises the following components in parts by weight: the liquid culture medium is prepared from 25g of maltose, 25g of yeast powder, 15g of Chinese yam, 15g of soybean meal, 15g of potato powder, 0.8g of triammonium citrate and 0.004g of vitamin B, and is prepared into 200-300ml of liquid culture medium.
Comparative example 1
The difference from the examples 1-3 is that the culture medium is a conventional comprehensive pda liquid culture medium, the soybean oil and the magnesium acetate tetrahydrate are replaced by abscisic acid, the concentration of the abscisic acid is 0.5-1.5 ppm/L, and the concentration of the abscisic acid is the concentration of the whole solution in S3.
Comparative example 2
The differences from examples 1 to 3 are: soybean oil and magnesium acetate tetrahydrate were not added.
Comparative example 3
The differences from examples 1 to 3 are: magnesium acetate tetrahydrate was not added.
Comparative example 4
The differences from examples 1 to 3 are: no soybean oil was added.
Phellinus linteus fermentation mycelia were cultured by the methods of examples 1-3 and comparative examples 1-4, respectively. The results show that:
the mycelium yields of examples 1-3 can reach 32.06-38.52g/L, wherein especially the yield of example 3 is the best, and the average of 5 repeated cultures of example 3 can reach 35.83 g/L.
Whereas the mycelium yield of comparative examples 1-4 was reduced to a different extent compared to examples 1-3, wherein comparative example 1 was reduced by about 10-20% compared to examples 1-3; comparative example 2 is about 5-15% lower than examples 1-3, comparative example 3 is about 8-12% lower than examples 1-3, and comparative example 4 is about 5-10% lower than examples 1-3; while the yields of comparative examples 3 and 4 increased by 5-10% over comparative example 2.
Therefore, the invention reasonably configures the culture medium suitable for the growth of the phellinus igniarius fermentation mycelium, and the yeanzhou is matched with the plant oil and the tetrahydrate magnesium acetate which are quantitatively added in the culture process, so that the yield of the phellinus igniarius fermentation mycelium is greatly improved.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (8)
1. A liquid fermentation method for improving the yield of phellinus igniarius mycelium is characterized by comprising the following steps:
s1: preparing a liquid culture medium for culturing phellinus igniarius, wherein the liquid culture medium comprises the following components in parts by weight: 20-30 parts of maltose, 20-30 parts of yeast powder, 10-20 parts of Chinese yam, 10-20 parts of soybean powder, 10-20 parts of potato powder, 0.5-1 part of triammonium citrate, 0.001-0.005 part of vitamin B and the balance of water;
s2, inoculating phellinus igniarius strains into a liquid culture medium, performing activated culture, then inoculating the phellinus igniarius strains into a seed culture medium according to the inoculation amount of 10-15% of the volume percentage, and performing seed culture to obtain seed liquid;
s3, inoculating the seed solution prepared in the step S2 into a liquid culture medium according to the inoculation amount of 10-15% in volume percentage, performing liquid fermentation culture for 1-3 days at the temperature of 28-35 ℃, adding a certain amount of vegetable oil and magnesium acetate tetrahydrate in any day of the culture process, and continuing to culture for 2-3 days at the temperature of 23-28 ℃ to obtain a mixed solution of phellinus igniarius mycelia and a fermentation liquid;
and S4, performing pressure filtration on the mixed liquid of the phellinus igniarius mycelia and the fermentation liquid obtained in the step II through a plate-and-frame filter press, drying a filter cake at the temperature of 70-80 ℃, performing vacuum concentration on the filtrate, performing spray drying on the filtrate in a spray dryer to obtain dry powder, and mixing the phellinus igniarius mycelia and the dry powder to obtain the phellinus igniarius fermentation mycelia.
2. The liquid fermentation method for improving the yield of phellinus linteus mycelium according to claim 1, wherein the liquid culture medium comprises the following components in parts by weight: 20 parts of maltose, 20 parts of yeast powder, 10 parts of Chinese yam, 10 parts of soybean meal, 10 parts of potato powder, 0.5 part of triammonium citrate, 0.002 part of vitamin B and the balance of water.
3. The liquid fermentation method for improving the yield of phellinus linteus mycelium according to claim 1, wherein the liquid culture medium comprises the following components in parts by weight: 30 parts of maltose, 30 parts of yeast powder, 20 parts of Chinese yam, 20 parts of soybean meal, 20 parts of potato powder, 1 part of triammonium citrate, 0.005 part of vitamin B and the balance of water.
4. The liquid fermentation method for improving the yield of phellinus linteus mycelium according to claim 1, wherein the liquid culture medium comprises the following components in parts by weight: 25 parts of maltose, 25 parts of yeast powder, 15 parts of Chinese yam, 15 parts of soybean meal, 15 parts of potato powder, 0.8 part of triammonium citrate, 0.004 part of vitamin B and the balance of water.
5. The liquid fermentation method for improving the yield of Phellinus linteus mycelium according to claim 1, wherein the vegetable oil is added in an amount of 2-10 g/L.
6. The liquid fermentation method for improving the yield of Phellinus linteus mycelium according to claim 1, wherein the magnesium acetate tetrahydrate is added in an amount of 0.5-1 g/L.
7. The liquid fermentation method for improving the yield of Phellinus linteus mycelium according to claim 5, wherein the vegetable oil is added in an amount of 5-8 g/L.
8. The liquid fermentation method for improving the yield of Phellinus linteus mycelium according to claim 1, wherein the culture conditions in S2 are: culturing at 25-28 deg.C for 3-5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011305933.3A CN112322572A (en) | 2020-11-19 | 2020-11-19 | Liquid fermentation method for increasing yield of phellinus igniarius mycelium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011305933.3A CN112322572A (en) | 2020-11-19 | 2020-11-19 | Liquid fermentation method for increasing yield of phellinus igniarius mycelium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112322572A true CN112322572A (en) | 2021-02-05 |
Family
ID=74321736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011305933.3A Pending CN112322572A (en) | 2020-11-19 | 2020-11-19 | Liquid fermentation method for increasing yield of phellinus igniarius mycelium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112322572A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114317295A (en) * | 2022-02-25 | 2022-04-12 | 内蒙古科学技术研究院 | Liquid culture medium for culturing phellinus igniarius mycelium and culture method thereof |
| CN114451216A (en) * | 2022-02-25 | 2022-05-10 | 内蒙古科学技术研究院 | Large-scale artificial cultivation method for phellinus igniarius sporocarp |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348803A (en) * | 2008-09-03 | 2009-01-21 | 西南大学 | Artificial nutrient medium for Phellinus linteus fluid culture and method for fermentation of Phellinus linteus polysaccharide |
| CN101933439A (en) * | 2010-07-15 | 2011-01-05 | 西南大学 | Method for improving phellinus igniarius hypha amount of submerged culture by utilizing plant oil |
| CN101983955A (en) * | 2010-08-13 | 2011-03-09 | 浙江省农业科学院 | Phellinus igniarius mycelium culture medium and process for fermenting phellinus igniarius using same |
| CN103820333A (en) * | 2014-03-10 | 2014-05-28 | 陕西省微生物研究所 | Liquid fermentation method for improving yield of phellinus fermentation mycelia |
| CN106119313A (en) * | 2016-06-29 | 2016-11-16 | 上海市农业科学院 | A kind of liquid fermentation method of phellinus igniarius mycelium |
| CN111937679A (en) * | 2020-09-01 | 2020-11-17 | 郝哲 | Phellinus igniarius culture medium and preparation method thereof |
-
2020
- 2020-11-19 CN CN202011305933.3A patent/CN112322572A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348803A (en) * | 2008-09-03 | 2009-01-21 | 西南大学 | Artificial nutrient medium for Phellinus linteus fluid culture and method for fermentation of Phellinus linteus polysaccharide |
| CN101933439A (en) * | 2010-07-15 | 2011-01-05 | 西南大学 | Method for improving phellinus igniarius hypha amount of submerged culture by utilizing plant oil |
| CN101983955A (en) * | 2010-08-13 | 2011-03-09 | 浙江省农业科学院 | Phellinus igniarius mycelium culture medium and process for fermenting phellinus igniarius using same |
| CN103820333A (en) * | 2014-03-10 | 2014-05-28 | 陕西省微生物研究所 | Liquid fermentation method for improving yield of phellinus fermentation mycelia |
| CN106119313A (en) * | 2016-06-29 | 2016-11-16 | 上海市农业科学院 | A kind of liquid fermentation method of phellinus igniarius mycelium |
| CN111937679A (en) * | 2020-09-01 | 2020-11-17 | 郝哲 | Phellinus igniarius culture medium and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 姜福春等: "乙酸镁对桑黄液态发酵合成黄酮类物质的促进研究", 《菌物学报》 * |
| 高慧娟等: "金属离子对桑黄菌丝体及胞外多糖含量的影响", 《食品研究与开发》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114317295A (en) * | 2022-02-25 | 2022-04-12 | 内蒙古科学技术研究院 | Liquid culture medium for culturing phellinus igniarius mycelium and culture method thereof |
| CN114451216A (en) * | 2022-02-25 | 2022-05-10 | 内蒙古科学技术研究院 | Large-scale artificial cultivation method for phellinus igniarius sporocarp |
| CN114451216B (en) * | 2022-02-25 | 2023-05-05 | 内蒙古科学技术研究院 | Large-scale artificial cultivation method for Phellinus linteus fruiting bodies |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103109679B (en) | Method for cultivating antrodia camphorate sporocarp basswood in the second stage | |
| CN102154407B (en) | Corayceps militaris polysaccharide two-stage fermentation synthesis process | |
| CN103988712A (en) | High-yield polysaccharide cordyceps militaris cultivation method | |
| CN102217487B (en) | Method for producing mycelia of Antrodia camphorata through deep liquid state fermentation | |
| CN112410228B (en) | Culture of inonotus obliquus biotransformation mycelium and application in reducing blood sugar | |
| CN112322572A (en) | Liquid fermentation method for increasing yield of phellinus igniarius mycelium | |
| CN106434373A (en) | High-density fermentation medium formula of sparassis crispa and pharmaceutical grade glucan preparation method of high-density fermentation medium formula | |
| CN103820333A (en) | Liquid fermentation method for improving yield of phellinus fermentation mycelia | |
| CN110684672B (en) | Fermentation method of antioxidant cordyceps sobolifera mycelium | |
| CN114317295A (en) | Liquid culture medium for culturing phellinus igniarius mycelium and culture method thereof | |
| CN103087893A (en) | Preparation method of composite coarse cereals monascus | |
| CN107432135A (en) | Promote the method for cynomorium songaricum seed sprouting using fungi | |
| CN105580638A (en) | Method for promoting antrodia camphorata liquid state fermentation growth and triterpene synthesis | |
| CN114015532B (en) | Monascus esterifying enzyme aroma-enhanced apple vinegar and preparation method thereof | |
| CN103820406A (en) | Method for producing laccase by solid fermentation of funalia trogii by using traditional Chinese medicine as matrix, and for comprehensive utilization of fermentation residues | |
| CN106361780A (en) | Fermentation method for improving yield of antrodia camphorata total triterpenes compound | |
| CN103146775A (en) | Method for preparing epigallocatechin-3-gallate and epigallocatechin gallate by liquid-submerged fermentation | |
| CN108901611A (en) | A kind of Antrodia camphorata culture medium and its preparation method and application | |
| CN101407767A (en) | Method for producing Chinese caterpillar fungus by fermentation | |
| CN110903983B (en) | Monascus monascus with high yield of saccharifying enzyme and esterifying enzyme and application thereof | |
| CN106497795A (en) | A kind of Cordyceps funguss seed culture medium containing Fructus Lycii and its application | |
| CN112189506A (en) | Method for efficiently cultivating phellinus igniarius sporocarp based on artificial bagged materials | |
| CN114276973B (en) | Method for promoting deep fermentation of Antrodia camphorata to produce spores and sugar by adding Antrodia camphorata extract | |
| CN104004815A (en) | Culture method of high-yield carotenoid cordyceps militaris | |
| CN104232727A (en) | Method for preparing germanium-enriched phellinus igniarius fermentation product |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |