CN114451216A - Large-scale artificial cultivation method for phellinus igniarius sporocarp - Google Patents
Large-scale artificial cultivation method for phellinus igniarius sporocarp Download PDFInfo
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- CN114451216A CN114451216A CN202210179375.3A CN202210179375A CN114451216A CN 114451216 A CN114451216 A CN 114451216A CN 202210179375 A CN202210179375 A CN 202210179375A CN 114451216 A CN114451216 A CN 114451216A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
According to the method, the hollow cylindrical plastic pipe is selected, the liquid culture medium is added into the hollow portion, the solid culture medium is filled into the two ends of the plastic pipe to prepare the fungus sticks, and then the phellinus igniarius sporocarp is cultured, so that the defects of wood waste, long growth period and insufficient nutrition, susceptibility to infection and high cost of the solid culture medium and the liquid culture medium are avoided, the advantages of the solid culture medium and the liquid culture medium are combined, the fungus sticks are easy to manufacture and low in cost, the probability of scratching fungus bags can be reduced, pollution is avoided, and a good foundation can be provided for a standardized culture process of the phellinus igniarius. The process can realize the control of standardized quality and technical parameters, and can realize mechanized, standardized, industrialized, large-scale and standardized artificial cultivation mode of phellinus igniarius.
Description
Technical Field
The invention belongs to the technical field of artificial cultivation of phellinus igniarius, and particularly relates to a large-scale artificial cultivation method of phellinus igniarius sporocarp.
Background
Phellinus linteus belongs to the class of Basidiomycetes of Phellinus linteus, also called Phellinus baumii, has become a research hotspot at home and abroad due to the multiple functions of resisting tumor, resisting oxidation and the like of the fruiting body, and is a fungus with the highest biological anticancer efficiency internationally acknowledged.
In recent years, the increase of the market demand for phellinus igniarius causes that the predatory exploitation of wild phellinus igniarius cannot be avoided, and is limited by the particularity, complexity and external conditions of the physiological ecology of the phellinus igniarius, so that fruiting bodies formed in the nature are very rare, the wild natural phellinus igniarius resources are deficient and face exhaustion, and in addition, the artificial domestication and cultivation of phellinus igniarius is difficult to form stable medical industry product sources, so that the development of the phellinus igniarius industry is greatly limited.
At present, two main artificial cultivation modes are available, one mode is mainly cultivation of mulberry trees, poplar trees and other cut-log trees, the cut-log cultivation needs a large amount of wood resources and is difficult to produce in a large scale, the other mode is cultivation in bags, the culture medium for cultivation in bags has the problems of insufficient nutrition, small sporocarp development and susceptibility to infectious microbes, and the solid culture medium has the problems of nonuniform strain dispersion and long growth period. Meanwhile, the liquid culture medium is adopted for artificial cultivation, so that the method has the advantages of convenience in inoculation, short cultivation period, low pollution rate, controllable and consistent inoculation amount, and rapid field planting, but the technical problems of single formula and high cost of the liquid culture medium still exist, and the large-scale application of the liquid culture medium is limited.
Therefore, how to select a proper culture medium and a proper culture method to shorten the artificial cultivation period of phellinus igniarius and reduce the production cost is still a difficult point which needs to be solved urgently by the technical personnel in the field.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the large-scale artificial cultivation method of the phellinus igniarius sporocarp, which realizes a shorter growth period of the phellinus igniarius sporocarp by adjusting the composition and the proportion of a culture medium and optimizing the process parameters in the cultivation method, reduces the production cost and can meet the requirements of large-scale production.
In order to achieve the purpose, the invention adopts the following technical scheme that the large-scale artificial cultivation method of phellinus igniarius sporocarp comprises the following steps:
(1) preparation of solid Medium
Weighing 20-40 parts of cottonseed hulls, 30-60 parts of mulberry sawdust, 5-10 parts of bran, 1-3 parts of glucose, 2-4 parts of sucrose, 1-2 parts of hydroxyapatite, 2-6 parts of monopotassium phosphate, 1-3 parts of calcium sulfate and 3-6 parts of sodium alginate, uniformly mixing, and controlling the water content to be 60-70% to obtain a solid culture medium;
(2) preparation of liquid Medium
Taking water as a solvent, wherein each liter of culture medium comprises the following raw materials by weight: 10-20g of corn flour, 2-5g of monopotassium phosphate, 1-3g of magnesium sulfate, 10-15g of glucose, 0.002-0.005g of vitamin B, 5-8g of peptone and 0.5-1g of magnesium acetate tetrahydrate; mixing the components with water uniformly to obtain a liquid culture medium;
(3) preparation of mushroom stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture media into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, and fastening two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) inoculating and spawning
Inoculating Phellinus Linteus strain on solid culture medium at two ends of the strain stick, performing dark culture at 25-30 deg.C and humidity of 60-70%, and stopping spawn running when the strain is full of two thirds of the strain bag;
(5) culturing in the presence of yellow pigment
Transferring the bacteria stick after bacteria germination into a greenhouse, controlling the temperature to be 25-30 ℃ and the humidity to be 80-90% for culturing under the condition that the opening is in a V-shaped cut, ventilating for 30-40min at an interval of 6h every day, controlling the concentration of CO2 to be 2500ppm, controlling the illumination to be 200-300lux, and culturing for 15-20 days;
adjusting the temperature to 25-30 deg.C, humidity to above 95%, CO2 concentration below 1500ppm, illumination at 200-;
(6) drying the mixture
Drying collected Phellinus linteus fruiting body in a drying oven at 50-60 deg.C for 10-12 hr to make water content of Phellinus linteus fruiting body below 1.3%.
The cottonseed hulls are prepared by crushing the cottonseed hulls and sieving the crushed cottonseed hulls with a sieve of 200 meshes and 300 meshes, the particle size of the mulberry sawdust is 0.5-2cm, and the particle size of the hydroxyapatite is 80-120 nm; the grain diameter of the corn flour is 100-200 meshes;
the length of the hollow cylindrical plastic pipe fitting is 20-35cm, the diameter of the hollow cylindrical plastic pipe fitting is 10-15cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate; the sterilization in the step (3) is performed for 10-20min under the pressure of 0.2-0.5MPa and at the temperature of 130-;
and (5) in the yellow culture in the step (5), a sun-shading net is arranged on the greenhouse, and a green film or a white film is added on the sun-shading net.
In the step (5) of yellow culture, the plastic partition plate is taken out, and the fungus stick is periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the method, the hollow cylindrical plastic pipe is selected, the liquid culture medium is added into the hollow portion, the solid culture medium is filled into the two ends of the plastic pipe to prepare the fungus sticks, and then the phellinus igniarius sporocarp is cultured, so that the defects of wood waste, long growth period and insufficient nutrition, susceptibility to infection and high cost of the solid culture medium and the liquid culture medium are avoided, the advantages of the solid culture medium and the liquid culture medium are combined, the fungus sticks are easy to manufacture and low in cost, the probability of scratching fungus bags can be reduced, pollution is avoided, and a good foundation can be provided for a standardized culture process of the phellinus igniarius.
(2) According to the invention, the composition and the proportion of the solid culture medium and the liquid culture medium are optimized, so that the using amount of the mulberry sawdust is reduced, the using amount of cottonseed hulls is increased, the requirement on wood resources is reduced, and meanwhile, the culture medium can contain nutrient substances required by or required to be enriched by phellinus igniarius, so that the rapid extension of phellinus igniarius filaments is facilitated, the culture period is shortened, and scientific, repeatable and stable research and improvement on the quality of phellinus igniarius sporocarp can be realized.
(3) The process can realize the control of standardized quality and technical parameters, and can realize mechanized, standardized, industrialized, large-scale and standardized artificial cultivation mode of phellinus igniarius.
Detailed Description
For a further understanding of the invention, reference will now be made to the preferred embodiments of the present invention by way of example, and it is to be understood that the description is intended to further illustrate features and advantages of the present invention and is not intended to limit the scope of the claims which follow. All starting materials for the present invention, without particular limitation as to their source, may be purchased commercially or prepared according to conventional methods well known to those skilled in the art.
Example 1
A large-scale artificial cultivation method of phellinus igniarius sporocarp comprises the following steps:
(1) preparation of solid Medium
Weighing 40 parts of cottonseed hulls, 30 parts of mulberry sawdust, 10 parts of bran, 3 parts of glucose, 2 parts of sucrose, 1 part of hydroxyapatite, 3 parts of monopotassium phosphate, 1 part of calcium sulfate and 6 parts of sodium alginate, uniformly mixing, and controlling the water content to be 70% to obtain a solid culture medium;
(2) preparation of liquid Medium
Taking water as a solvent, wherein each liter of culture medium comprises the following raw materials by weight: 10g of corn flour, 5g of monopotassium phosphate, 3g of magnesium sulfate, 15g of glucose, 0.005g of vitamin B, 5g of peptone and 0.5g of magnesium acetate tetrahydrate; mixing the components with water uniformly to obtain a liquid culture medium;
(3) preparation of mushroom stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture media into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, and fastening two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) inoculating and spawning
Inoculating Phellinus Linteus strain on solid culture medium at two ends of the strain stick, performing dark culture at 25 deg.C and humidity of 70%, and stopping spawn running when the strain is full of two thirds of the strain bag;
(5) culturing in the presence of yellow pigment
Transferring the fungus sticks after fungus growing into a greenhouse, controlling the temperature to be 30 ℃ and the humidity to be 80% for culturing, ventilating for 30min at intervals of 6h every day, controlling the concentration of CO2 to be 2000ppm and controlling the illumination to be 200lux, and culturing for 15 days;
adjusting temperature to 30 deg.C, humidity to above 95%, CO2 concentration below 1500ppm, light illumination to 300lux, ventilating for 40min at 4h every day, and culturing until fruiting body is mature;
(6) drying
Drying collected Phellinus Linteus fruiting body in a drying oven at 60 deg.C for 11 hr to make water content of Phellinus Linteus fruiting body below 1.3%.
The cottonseed hulls are prepared by crushing and screening through a 200-mesh screen, the particle size of the mulberry sawdust is 0.5cm, and the particle size of the hydroxyapatite is 120 nm; the grain size of the corn flour is 200 meshes;
the length of the hollow cylindrical plastic pipe fitting is 35cm, the diameter of the hollow cylindrical plastic pipe fitting is 15cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate; in the step (3), the sterilization is performed for 10min under the pressure of 0.5MPa and at the temperature of 140 ℃;
in the yellow cultivation in the step (5), a sun-shading net is arranged on the greenhouse, and a green-white film is added on the sun-shading net; in the step (5) of yellow culture, the plastic partition plate is taken out, and the fungus stick is periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
Example 2
A large-scale artificial cultivation method of phellinus igniarius sporocarp comprises the following steps:
(1) preparation of solid Medium
Weighing 20 parts of cottonseed hulls, 60 parts of mulberry sawdust, 10 parts of bran, 3 parts of glucose, 2 parts of sucrose, 1 part of hydroxyapatite, 2 parts of monopotassium phosphate, 1 part of calcium sulfate and 4 parts of sodium alginate, uniformly mixing, and controlling the water content to be 65% to obtain a solid culture medium;
(2) preparation of liquid Medium
Taking water as a solvent, wherein each liter of culture medium comprises the following raw materials by weight: 15g of corn flour, 5g of monopotassium phosphate, 1g of magnesium sulfate, 12g of glucose, 0.004g of vitamin B, 8g of peptone and 1g of magnesium acetate tetrahydrate; mixing the components with water uniformly to obtain a liquid culture medium;
(3) preparation of mushroom stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture media into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, and fastening two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) inoculating and spawning
Inoculating Phellinus Linteus strain on solid culture medium at two ends of the strain stick, performing dark culture at 28 deg.C and humidity of 70%, and stopping spawn running when the strain is full of two thirds of the strain bag;
(5) culturing in the presence of yellow pigment
Transferring the fungus sticks after fungus growing into a greenhouse, controlling the temperature to be 28 ℃ and the humidity to be 85%, culturing at the V-shaped cut, ventilating for 30min at intervals of 6h every day, controlling the concentration of CO2 to be 2500ppm, controlling the illumination to be 250lux, and culturing for 20 days;
adjusting temperature to 30 deg.C, humidity to above 95%, CO2 concentration to below 1500ppm, controlling illumination at 200lux, ventilating for 40min at 4h every day, and continuously culturing until fruiting body is mature;
(6) drying
Drying the collected phellinus igniarius sporocarp in a constant temperature drying box at 50 ℃ for 12 hours to ensure that the water content of the phellinus igniarius sporocarp reaches below 1.3 percent.
The cottonseed hulls are prepared by crushing and screening through a 300-mesh screen, the particle size of the mulberry sawdust is 2cm, and the particle size of the hydroxyapatite is 120 nm; the grain size of the corn flour is 200 meshes;
the length of the hollow cylindrical plastic pipe is 20cm, the diameter of the hollow cylindrical plastic pipe is 10cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate; the sterilization in the step (3) adopts sterilization for 10min under the pressure of 0.5MPa and the temperature of 130 ℃; and (3) in the step (5) of yellow cultivation, arranging a sunshade net on the greenhouse, and adding a white film on the sunshade net, in the step (5) of yellow cultivation, taking out the plastic partition plate, and periodically shaking the fungus stick to promote the liquid culture medium to permeate into the solid culture medium.
Example 3
A large-scale artificial cultivation method of phellinus igniarius sporocarp comprises the following steps:
(1) preparation of solid Medium
Weighing 30 parts of cottonseed hulls, 50 parts of mulberry sawdust, 6 parts of bran, 2 parts of glucose, 3 parts of sucrose, 1 part of hydroxyapatite, 5 parts of monopotassium phosphate, 3 parts of calcium sulfate and 6 parts of sodium alginate, uniformly mixing, and controlling the water content to be 70% to obtain a solid culture medium;
(2) preparation of liquid Medium
Taking water as a solvent, wherein each liter of culture medium comprises the following raw materials by weight: 15g of corn flour, 3g of monopotassium phosphate, 3g of magnesium sulfate, 10g of glucose, 0.005g of vitamin B, 8g of peptone and 0.5g of magnesium acetate tetrahydrate; mixing the components with water uniformly to obtain a liquid culture medium;
(3) preparation of mushroom stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture media into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, and fastening two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) inoculating and spawning
Inoculating Phellinus Linteus strain on solid culture medium at two ends of the strain stick, performing dark culture at 26 deg.C and humidity of 65%, and stopping spawn running when the strain is full of two thirds of the strain bag;
(5) culturing in the presence of yellow pigment
Transferring the fungus sticks after fungus growing into a greenhouse, controlling the temperature to be 27 ℃ and the humidity to be 85%, culturing at the V-shaped cut, ventilating for 35min at intervals of 6h every day, controlling the concentration of CO2 to be 2200ppm and controlling the illumination to be 260lux, and culturing for 18 days;
adjusting temperature to 30 deg.C, humidity to above 95%, CO2 concentration below 1500ppm, light illumination to 300lux, ventilating for 40min at 4h every day, and culturing until fruiting body is mature;
(6) drying
Drying collected Phellinus Linteus fruiting body in a drying oven at 55 deg.C for 10 hr to make water content of Phellinus Linteus fruiting body below 1.3%.
The cottonseed hulls are prepared by crushing and screening through a 250-mesh screen, the particle size of the mulberry sawdust is 1cm, and the particle size of the hydroxyapatite is 100 nm; the grain size of the corn flour is 150 meshes;
the length of the hollow cylindrical plastic pipe fitting is 30cm, the diameter of the hollow cylindrical plastic pipe fitting is 12cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate; in the step (3), sterilization is carried out for 15min under the pressure of 0.3MPa and at the temperature of 135 ℃;
in the yellow cultivation in the step (5), a sun-shading net is arranged on the greenhouse, and a green-white film is added on the sun-shading net; in the step (5) of yellow culture, the plastic partition plate is taken out, and the fungus stick is periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
Example 4
A large-scale artificial cultivation method of phellinus igniarius sporocarp comprises the following steps:
(1) preparation of solid Medium
Weighing 28 parts of cottonseed hulls, 42 parts of mulberry sawdust, 7 parts of bran, 2 parts of glucose, 4 parts of sucrose, 2 parts of hydroxyapatite, 6 parts of monopotassium phosphate, 3 parts of calcium sulfate and 4 parts of sodium alginate, uniformly mixing, and controlling the water content to be 60% to obtain a solid culture medium;
(2) preparation of liquid Medium
Taking water as a solvent, wherein each liter of culture medium comprises the following raw materials by weight: 18g of corn flour, 5g of monopotassium phosphate, 3g of magnesium sulfate, 10g of glucose, 0.003g of vitamin B, 8g of peptone and 1g of magnesium acetate tetrahydrate; mixing the components with water uniformly to obtain a liquid culture medium;
(3) preparation of mushroom stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture media into two ends of the plastic pipe fitting, filling the plastic pipe fitting into a polypropylene bag, and fastening two ends to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) inoculating and spawn running
Inoculating Phellinus Linteus strain on solid culture medium at two ends of the strain stick, performing dark culture at 30 deg.C and humidity of 70%, and stopping spawn running when the strain is full of two thirds of the strain bag;
(5) culturing in the presence of yellow pigment
Transferring the fungus sticks after fungus growing into a greenhouse, controlling the temperature to be 258 ℃ and the humidity to be 80% for culturing, ventilating for 30min at 6h intervals every day, controlling the concentration of CO2 to be 2300ppm, controlling the illumination to be 240lux, and culturing for 17 days;
adjusting temperature to 30 deg.C, humidity to above 95%, CO2 concentration below 1500ppm, illumination to 230lux, ventilating for 40min at 4h every day, and continuously culturing until fruiting body is mature;
(6) drying
Drying collected Phellinus Linteus fruiting body in a drying oven at 60 deg.C for 10 hr to make water content of Phellinus Linteus fruiting body below 1.3%.
The cottonseed hulls are prepared by crushing and screening through a 200-mesh screen, the particle size of the mulberry sawdust is 1.5cm, and the particle size of the hydroxyapatite is 90 nm; the grain size of the corn flour is 180 meshes;
the length of the hollow cylindrical plastic pipe is 32cm, the diameter of the hollow cylindrical plastic pipe is 13cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate; in the step (3), the sterilization is performed for 20min under the pressure of 0.4MPa and at the temperature of 140 ℃;
in the yellow cultivation in the step (5), a sunshade net is arranged on the greenhouse, and a white film is added on the upper surface of the sunshade net; in the step (5) of yellow culture, the plastic partition plate is taken out, and the fungus stick is periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
It will be understood by those skilled in the art that the foregoing is only a preferred embodiment of the present invention, and is not intended to limit the invention, and that any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (9)
1. A large-scale artificial cultivation method of phellinus igniarius sporocarp is characterized by comprising the following steps: the method comprises the following steps:
(1) preparation of solid Medium
Weighing 20-40 parts of cottonseed hulls, 30-60 parts of mulberry sawdust, 5-10 parts of bran, 1-3 parts of glucose, 2-4 parts of sucrose, 1-2 parts of hydroxyapatite, 2-6 parts of monopotassium phosphate, 1-3 parts of calcium sulfate and 3-6 parts of sodium alginate, uniformly mixing, and controlling the water content to be 60-70% to obtain a solid culture medium;
(2) preparation of liquid Medium
Taking water as a solvent, wherein each liter of culture medium comprises the following raw materials by weight: 10-20g of corn flour, 2-5g of monopotassium phosphate, 1-3g of magnesium sulfate, 10-15g of glucose, 0.002-0.005g of vitamin B, 5-8g of peptone and 0.5-1g of magnesium acetate tetrahydrate; mixing the components with water uniformly to obtain a liquid culture medium;
(3) preparation of mushroom stick
Selecting a hollow cylindrical plastic pipe fitting, adding the liquid culture medium into the hollow part, filling solid culture media at two ends of the plastic pipe fitting, filling the plastic pipe fitting into polypropylene bags, and fastening two ends of the plastic pipe fitting to obtain a fungus stick, and sterilizing the fungus stick for later use;
(4) inoculating and spawning
Inoculating Phellinus Linteus strain on solid culture medium at two ends of the strain stick, performing dark culture at 25-30 deg.C and humidity of 60-70%, and stopping spawn running when the strain is full of two thirds of the strain bag;
(5) culturing in the presence of yellow pigment
Transferring the bacteria stick after bacteria germination into a greenhouse, controlling the temperature to be 25-30 ℃ and the humidity to be 80-90% for culturing under the condition that the opening is in a V-shaped cut, ventilating for 30-40min at an interval of 6h every day, controlling the concentration of CO2 to be 2500ppm, controlling the illumination to be 200-300lux, and culturing for 15-20 days;
adjusting the temperature to 25-30 deg.C, humidity to above 95%, CO2 concentration below 1500ppm, illumination at 200-;
(6) drying
Drying collected Phellinus linteus fruiting body in a drying oven at 50-60 deg.C for 10-12 hr to make water content of Phellinus linteus fruiting body below 1.3%.
2. The large-scale artificial cultivation method of phellinus igniarius sporocarp according to claim 1, which is characterized in that: the cottonseed hulls are prepared by crushing and sieving through a 200-mesh and 300-mesh sieve.
3. The large-scale artificial cultivation method of phellinus igniarius sporocarp according to claim 1, which is characterized in that: the particle size of the mulberry sawdust is 0.5-2 cm.
4. The large-scale artificial cultivation method of phellinus igniarius sporocarp according to claim 1, which is characterized in that: the particle size of the hydroxyapatite is 80-120 nm.
5. The large-scale artificial cultivation method of phellinus igniarius sporocarp according to claim 1, which is characterized in that: the grain diameter of the corn flour is 100-200 meshes.
6. The large-scale artificial cultivation method of phellinus igniarius sporocarp according to claim 1, which is characterized in that: the length of the hollow cylindrical plastic pipe is 20-35cm, the diameter is 10-15cm, and the liquid culture medium and the solid culture medium are separated by a plastic partition plate.
7. The large-scale artificial cultivation method of phellinus igniarius sporocarp according to claim 1, which is characterized in that: the sterilization in the step (3) is performed for 10-20min under the pressure of 0.2-0.5MPa and at the temperature of 130-.
8. The large-scale artificial cultivation method of phellinus igniarius sporocarp according to claim 1, which is characterized in that: and (5) in the yellow culture in the step (5), a sun-shading net is arranged on the greenhouse, and a green film or a white film is added on the sun-shading net.
9. The large-scale artificial cultivation method of phellinus igniarius sporocarp according to claim 1, which is characterized in that: in the step (5), the plastic partition plate is taken out, and the fungus stick is periodically shaken to promote the liquid culture medium to permeate into the solid culture medium.
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| CN113044284A (en) * | 2019-12-26 | 2021-06-29 | 成都宏恩生物科技有限公司 | Preparation method of solid-liquid separation toothpaste and corresponding container structure |
| CN112514734A (en) * | 2020-10-31 | 2021-03-19 | 贵州省农作物品种资源研究所(贵州省现代中药材研究所) | Indoor cultivation method for phellinus igniarius |
| CN112322572A (en) * | 2020-11-19 | 2021-02-05 | 陕西省微生物研究所 | Liquid fermentation method for increasing yield of phellinus igniarius mycelium |
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