CN103087893A - Preparation method of composite coarse cereals monascus - Google Patents

Preparation method of composite coarse cereals monascus Download PDF

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CN103087893A
CN103087893A CN2013100140619A CN201310014061A CN103087893A CN 103087893 A CN103087893 A CN 103087893A CN 2013100140619 A CN2013100140619 A CN 2013100140619A CN 201310014061 A CN201310014061 A CN 201310014061A CN 103087893 A CN103087893 A CN 103087893A
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monascus
rice
red
substratum
food
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CN2013100140619A
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CN103087893B (en
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宋春雪
赵红年
曹晋宜
丁静
张敏
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山西梁汾醋业有限公司
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Abstract

The invention discloses a preparation method of composite coarse cereals monascus, belonging to the field of bioengineering microbial fermentation. The preparation method comprises the following steps of: respectively inoculating and culturing pigmental monascus, ester-producing monascus and functional monascus on a PDA (Potato Dextrose Agar) culture medium; preparing a spore suspension liquid from a bacterial strain inclined culture medium, and inoculating a seed culture fluid into the spore suspension liquid to carry out culturing; inoculating a pigmental monascus liquid seed on a sorghum rice culture medium, inoculating a functional monascus liquid seed on a myotonin culture medium, inoculating a functional monascus liquid seed on a buckwheat culture medium, inoculating an ester-producing monascus liquid seed on a millet culture medium, and respectively culturing in a stacking way to obtain four monascus; and mixing the four monascus to prepare the composite coarse cereals monascus, wherein the mass ratio of sorghum rice monascus to myotonin monascus to buckwheat monascus to millet monascus is 17:30:30:23. With the adoption of the preparation method, contents of monacolin-K, flavonoid matters and the like in a product are increased, so that the health efficacy of reducing blood fat of mature vinegar is strengthened when the flavor and the quality of the mature vinegar are ensured.

Description

A kind of preparation method of composite coarse grains red colouring agent for food, also used as a Chinese medicine

Technical field

The invention belongs to biotechnology microbial fermentation field, be specifically related to a kind of preparation method of composite coarse grains red colouring agent for food, also used as a Chinese medicine.

Background technology

Monascus can produce amylase, saccharifying enzyme, proteolytic enzyme, monascorubin etc. in yeast making process, can produce succsinic acid, citric acid and lovastatin isoreactivity material in fermenting process, being applied to vinegar brewing industry can increase the color and luster local flavor of seasonings, improve the quality structure of seasonings, improve product specification, make it have stronger nourishing function.

Red colouring agent for food, also used as a Chinese medicine is that monascus is bred the goods on rice or other food crop, can be used as medicinal and food colorant, China before more than 600 years with regard to producing as food, with a long history.

For a long time, the microorganism worker takes much count of research, the utilization to red colouring agent for food, also used as a Chinese medicine both at home and abroad, but mostly concentrates on traditional wine brewing, vinegar processed, tinting material, and about producing the research of the aspects such as succsinic acid, citric acid, and its pharmaceutical use is not subject to due attention.Japan rattan chapter professor far away isolated cholesterol synthesis inhibitor MonacolinK from red monascus Monascusruber in 1979, Chinese scholars begins it is carried out the discussion of medicinal biological value, and utilizes the modern biochemistry technology that its effective physiologically active substance is extracted, separates, analyzes.In recent years, functional factor in monascus (as biologically active substances such as cholesterol inhibitor, hypotensive hypoglycemic material, ergosterol and Substances) and toxin etc., cause just gradually scholars' interest, its pharmaceutical use also obtains people's attention thereupon gradually.

At present, the lipopenicillinase class medicine that at home and abroad occurs on market has lovastatin, Simvastatin, Pravastatin, fluvastatin, atorvastatin, Cerivastatin, zhibituo, Xuezhikang etc., and their reducing blood-fat mechanism is all relevant with Monacolin K.Thereby the edible vinegar that utilizes the red colouring agent for food, also used as a Chinese medicine exploitation to possess the antilipemic healthy effect has a extensive future.

Summary of the invention

The preparation method who the purpose of this invention is to provide a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine is to reach the purpose of utilizing the red colouring agent for food, also used as a Chinese medicine exploitation to possess the vinegar of antilipemic healthy effect.

Invention thinking of the present invention is: by take sorghum rice, seed of Job's tears, bitter buckwheat, millet as starter-making materials, inoculate respectively pigmental Monascus, functional Monascus mould, produce the ester monascus specie and make single grain red colouring agent for food, also used as a Chinese medicine, carry out composite, thereby make the composite coarse grains red colouring agent for food, also used as a Chinese medicine.

The present invention is achieved by the following technical solutions:

A kind of preparation method of composite coarse grains red colouring agent for food, also used as a Chinese medicine comprises the following steps:

(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 5-6 days on the PDA substratum that contains 2% glucose, 1% maltose, 2% agar, inoculation is produced the ester monascus in 30 ℃ of cultivations 10-12 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;

(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is washed spore with sterilized water make spore suspension, the inoculum size access seed culture fluid with 6% is cultivated 48-72h for 32 ℃ on the rotary type shaking table of 200r/min;

Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2O, 0.15%KH 2PO 4, 121 ℃ of sterilization 15min;

The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2O, lactic acid adjust pH 4.5;

Produce ester monascus liquid seeds nutrient solution: liquid glucose lO oBx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2PO 4

(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3-4h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;

pigmental Monascus liquid seeds with its quality 10% of access on the sorghum rice substratum, the mould liquid seeds of functional Monascus of its quality 10% of access on the seed of Job's tears substratum, the mould liquid seeds of functional Monascus of its quality 10% of access on the buckwheat substratum, the product ester monascus liquid seeds of its quality 10% of access on the millet substratum, the difference pile, keep spreading 24-48h out after 36-48h, surface red aspergillus hyphae spot covers with substantially, shakeout, turn over Qu Yici every 6-8h, and maintenance moisture, be cultured to 6-15d, can go out bent dry, obtaining water content is four kinds of red colouring agent for food, also used as a Chinese medicine of 10%-12%,

(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein the sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, namely gets the composite coarse grains red colouring agent for food, also used as a Chinese medicine.

Further, the pigmental Monascus liquid seeds of its quality 10% of access on the sorghum rice substratum for preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steams the red point of rice surface appearance, and spread the song material a little out this moment, again after 24h, temperature can rise to 42-45 ℃, steams rice surface red aspergillus hyphae spot and substantially covers with, and shakeout the song material this moment, turn over Qu Yici every 6-8h, carry out appropriate moisturizing with atomizer, be cultured to 6-7 days, can go out bent dry.

On the seed of Job's tears substratum for preparing its quality 10% of access the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 36-38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6-8h, carries out appropriate moisturizing with atomizer, be cultured to 12-15 days, can go out bent dry.

Access its quality 10% on the buckwheat substratum for preparing) the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 36-38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6-8h, carries out appropriate moisturizing with atomizer, be cultured to 12-15 days, can go out bent dry.

On the millet substratum for preparing its quality 10% of access product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 40h, temperature can rise to 40-43 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6-8h, carries out appropriate moisturizing with atomizer, be cultured to 8-10 days, can go out bent dry.

The composite coarse grains red colouring agent for food, also used as a Chinese medicine coupling effect that traditional Daqu and the present invention make is analyzed and researched:

With reference to traditional Shanxi mature vinegar technique, adding 54.5%(to calculate with staple food grain) the basis of the special-purpose Daqu of Shanxi mature vinegar on, introduce the composite coarse grains red colouring agent for food, also used as a Chinese medicine of 8% (calculating with staple food grain), with song for grain, carry out brewageing of old vinegar, through a large amount of research and tests, having extracted altogether the product of 10 batches analyzes and detects in strict accordance with standard, getting at last the mean value of 10 batch datas analyzes and compares, result such as table 1 with the index of not adding the old vinegar blank product of composite coarse grains red colouring agent for food, also used as a Chinese medicine gained:

Table 1

Compared with prior art, introduce the composite coarse grains red colouring agent for food, also used as a Chinese medicine that the functional Monascus mould species makes in the present invention, improved the content of Monacolin K, Flavonoid substances etc. in the product, in the local flavor and quality that guarantee old vinegar, strengthen the health-care effect of old vinegar reducing blood-fat, had wide market outlook.

Description of drawings

Fig. 1 is FB(flow block) of the present invention.

Embodiment

Embodiment 1

A kind of preparation method of composite coarse grains red colouring agent for food, also used as a Chinese medicine comprises the following steps:

(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 5 days on the PDA substratum that contains 2% glucose, 1% maltose, 2% agar, inoculation is produced the ester monascus in 30 ℃ of cultivations 11 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;

(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is washed spore with sterilized water make spore suspension, the inoculum size access seed culture fluid with 6% is cultivated 48h for 32 ℃ on the rotary type shaking table of 200r/min;

Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2O, 0.15%KH 2PO 4, 121 ℃ of sterilization 15min;

The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2O, lactic acid adjust pH 4.5;

Produce ester monascus liquid seeds nutrient solution: liquid glucose lO oBx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2PO 4

(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;

The pigmental Monascus liquid seeds of its quality 10% of access on the sorghum rice substratum for preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 24h, temperature can rise to 42 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 7h, carries out appropriate moisturizing with atomizer, be cultured to 6 days, can go out bent dry;

On the seed of Job's tears substratum for preparing its quality 10% of access the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 37 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6h, carries out appropriate moisturizing with atomizer, be cultured to 12 days, can go out bent dry;

The mould liquid seeds of functional Monascus of its quality 10% of access on the buckwheat substratum for preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 36 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 7h, carries out appropriate moisturizing with atomizer, be cultured to 13 days, can go out bent dry;

On the millet substratum for preparing its quality 10% of access product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 40h, temperature can rise to 40 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6h, carries out appropriate moisturizing with atomizer, be cultured to 10 days, can go out bent dry;

(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein the sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, namely gets the composite coarse grains red colouring agent for food, also used as a Chinese medicine.

Embodiment 2

A kind of preparation method of composite coarse grains red colouring agent for food, also used as a Chinese medicine comprises the following steps:

(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 5.5 days on the PDA substratum that contains 2% glucose, 1% maltose, 2% agar, inoculation is produced the ester monascus in 30 ℃ of cultivations 12 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;

(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is washed spore with sterilized water make spore suspension, the inoculum size access seed culture fluid with 6% is cultivated 55h for 32 ℃ on the rotary type shaking table of 200r/min;

Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2O, 0.15%KH 2PO 4, 121 ℃ of sterilization 15min;

The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2O, lactic acid adjust pH 4.5;

Produce ester monascus liquid seeds nutrient solution: liquid glucose lO oBx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2PO 4

(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3.5h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;

The pigmental Monascus liquid seeds of its quality 10% of access on the sorghum rice substratum for preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 24h, temperature can rise to 43 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6h, carries out appropriate moisturizing with atomizer, be cultured to 6.5 days, can go out bent dry;

On the seed of Job's tears substratum for preparing its quality 10% of access the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 8h, carries out appropriate moisturizing with atomizer, be cultured to 13 days, can go out bent dry;

The mould liquid seeds of functional Monascus of its quality 10% of access on the buckwheat substratum for preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6h, carries out appropriate moisturizing with atomizer, be cultured to 15 days, can go out bent dry;

On the millet substratum for preparing its quality 10% of access product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 40h, temperature can rise to 41 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 8h, carries out appropriate moisturizing with atomizer, be cultured to 9 days, can go out bent dry;

(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein the sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, namely gets the composite coarse grains red colouring agent for food, also used as a Chinese medicine.

Embodiment 3

A kind of preparation method of composite coarse grains red colouring agent for food, also used as a Chinese medicine comprises the following steps:

(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 6 days on the PDA substratum that contains 2% glucose, 1% maltose, 2% agar, inoculation is produced the ester monascus in 30 ℃ of cultivations 10 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;

(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is washed spore with sterilized water make spore suspension, the inoculum size access seed culture fluid with 6% is cultivated 72h for 32 ℃ on the rotary type shaking table of 200r/min;

Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2O, 0.15%KH 2PO 4, 121 ℃ of sterilization 15min;

The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2O, lactic acid adjust pH 4.5;

Produce ester monascus liquid seeds nutrient solution: liquid glucose lO oBx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2PO 4

(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 4h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;

The pigmental Monascus liquid seeds of its quality 10% of access on the sorghum rice substratum for preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 24h, temperature can rise to 44 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 8h, carries out appropriate moisturizing with atomizer, be cultured to 7 days, can go out bent dry;

On the seed of Job's tears substratum for preparing its quality 10% of access the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 36 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 7h, carries out appropriate moisturizing with atomizer, be cultured to 15 days, can go out bent dry;

The mould liquid seeds of functional Monascus of its quality 10% of access on the buckwheat substratum for preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 37 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6h, carries out appropriate moisturizing with atomizer, be cultured to 12 days, can go out bent dry;

On the millet substratum for preparing its quality 10% of access product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 40h, temperature can rise to 43 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 7h, carries out appropriate moisturizing with atomizer, be cultured to 8 days, can go out bent dry;

(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein the sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, namely gets the composite coarse grains red colouring agent for food, also used as a Chinese medicine.

Embodiment 4

A kind of preparation method of composite coarse grains red colouring agent for food, also used as a Chinese medicine comprises the following steps:

(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 6 days on the PDA substratum that contains 2% glucose, 1% maltose, 2% agar, inoculation is produced the ester monascus in 30 ℃ of cultivations 12 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;

(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is washed spore with sterilized water make spore suspension, the inoculum size access seed culture fluid with 6% is cultivated 60h for 32 ℃ on the rotary type shaking table of 200r/min;

Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2O, 0.15%KH 2PO 4, 121 ℃ of sterilization 15min;

The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2O, lactic acid adjust pH 4.5;

Produce ester monascus liquid seeds nutrient solution: liquid glucose lO oBx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2PO 4

(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;

The pigmental Monascus liquid seeds of its quality 10% of access on the sorghum rice substratum for preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 24h, temperature can rise to 45 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 7h, carries out appropriate moisturizing with atomizer, be cultured to 6.5 days, can go out bent dry;

On the seed of Job's tears substratum for preparing its quality 10% of access the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 37 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 7h, carries out appropriate moisturizing with atomizer, be cultured to 14 days, can go out bent dry;

The mould liquid seeds of functional Monascus of its quality 10% of access on the buckwheat substratum for preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 37 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 8h, carries out appropriate moisturizing with atomizer, be cultured to 14 days, can go out bent dry;

Mix and connect the sort of quyi: on the millet substratum for preparing its quality 10% of access product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 40h, temperature can rise to 42 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 8h, carries out appropriate moisturizing with atomizer, be cultured to 10 days, can go out bent dry;

(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein the sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, namely gets the composite coarse grains red colouring agent for food, also used as a Chinese medicine.

Claims (5)

1. the preparation method of a composite coarse grains red colouring agent for food, also used as a Chinese medicine, is characterized in that, comprises the following steps:
(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 5-6 days on the PDA substratum that contains 2% glucose, 1% maltose, 2% agar, inoculation is produced the ester monascus in 30 ℃ of cultivations 10-12 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;
(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is washed spore with sterilized water make spore suspension, the inoculum size access seed culture fluid with 6% is cultivated 48-72h for 32 ℃ on the rotary type shaking table of 200r/min;
Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2O, 0.15%KH 2PO 4, 121 ℃ of sterilization 15min;
The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2O, lactic acid adjust pH 4.5;
Produce ester monascus liquid seeds nutrient solution: liquid glucose lO oBx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2PO 4
(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3-4h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;
pigmental Monascus liquid seeds with its quality 10% of access on the sorghum rice substratum, the mould liquid seeds of functional Monascus of its quality 10% of access on the seed of Job's tears substratum, the mould liquid seeds of functional Monascus of its quality 10% of access on the buckwheat substratum, the product ester monascus liquid seeds of its quality 10% of access on the millet substratum, the difference pile, keep spreading 24-48h out after 36-48h, surface red aspergillus hyphae spot covers with substantially, shakeout, turn over Qu Yici every 6-8h, and maintenance moisture, be cultured to 6-15, can go out bent dry, obtaining water content is four kinds of red colouring agent for food, also used as a Chinese medicine of 10%-12%,
(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein the sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, namely gets the composite coarse grains red colouring agent for food, also used as a Chinese medicine.
2. the preparation method of a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine according to claim 1, it is characterized in that, the pigmental Monascus liquid seeds of its quality 10% of access on the sorghum rice substratum for preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 24h, temperature can rise to 42-45 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6-8h, carries out appropriate moisturizing with atomizer, be cultured to 6-7 days, can go out bent dry.
3. the preparation method of a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine according to claim 1 and 2, it is characterized in that, on the seed of Job's tears substratum for preparing its quality 10% of access the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 36-38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6-8h, carries out appropriate moisturizing with atomizer, be cultured to 12-15 days, can go out bent dry.
4. the preparation method of a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine according to claim 1 and 2, it is characterized in that, the mould liquid seeds of functional Monascus of its quality 10% of access on the buckwheat substratum for preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 48h, temperature can rise to 36-38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6-8h, carries out appropriate moisturizing with atomizer, be cultured to 12-15 days, can go out bent dry.
5. the preparation method of a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine according to claim 1 and 2, it is characterized in that, on the millet substratum for preparing its quality 10% of access product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam the red point of rice surface appearance, spread the song material a little out this moment, then after 40h, temperature can rise to 40-43 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, shakeout the song material this moment, turns over Qu Yici every 6-8h, carries out appropriate moisturizing with atomizer, be cultured to 8-10 days, can go out bent dry.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105104986A (en) * 2015-07-08 2015-12-02 成都大学 Preparation method of tartary buckwheat red yeast rice
CN105524792A (en) * 2016-02-03 2016-04-27 浙江红石梁集团济公家酒坊有限公司 Preparation method of mixed distiller's yeast
CN105695514A (en) * 2015-08-26 2016-06-22 泸州老窖集团养生酒业有限责任公司 Purple rice red yeast and preparation method and application thereof
CN105886315A (en) * 2016-05-12 2016-08-24 山西紫林醋业股份有限公司 Method for brewing super-mature vinegar by adopting red yeast prepared from tartary buckwheat
CN108085343A (en) * 2018-01-16 2018-05-29 福建省农业科学院农业工程技术研究所 The production technology of fermented grain functional Monascus powder

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001204460A (en) * 2000-01-28 2001-07-31 Gunze Ltd Monascus purpreus and method for preparation of the same
US20040081663A1 (en) * 2002-10-25 2004-04-29 Chun-Min Chang Pharmaceutical composition for treatment and prevention of cancer and the preparation thereof
KR20040051783A (en) * 2002-12-13 2004-06-19 이성구 Buckwheat fermented with fungi monascus species
CN101475912A (en) * 2008-10-13 2009-07-08 丁予章 Red koji strain and use thereof
CN101649296A (en) * 2009-09-23 2010-02-17 山东中惠食品有限公司 Novel monascus strain and monascus corn food prepared from same
CN102429163A (en) * 2011-11-02 2012-05-02 杭州双马生物工程有限公司 Method for preparing tartary buckwheat monascus and application thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001204460A (en) * 2000-01-28 2001-07-31 Gunze Ltd Monascus purpreus and method for preparation of the same
US20040081663A1 (en) * 2002-10-25 2004-04-29 Chun-Min Chang Pharmaceutical composition for treatment and prevention of cancer and the preparation thereof
KR20040051783A (en) * 2002-12-13 2004-06-19 이성구 Buckwheat fermented with fungi monascus species
CN101475912A (en) * 2008-10-13 2009-07-08 丁予章 Red koji strain and use thereof
CN101649296A (en) * 2009-09-23 2010-02-17 山东中惠食品有限公司 Novel monascus strain and monascus corn food prepared from same
CN102429163A (en) * 2011-11-02 2012-05-02 杭州双马生物工程有限公司 Method for preparing tartary buckwheat monascus and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
周立平: "红曲霉在中国的生产及其应用(下)", 《中国酿造》, no. 10, 31 December 2004 (2004-12-31), pages 6 - 3 *
张秀媛,等: "固体发酵制备张杂谷红曲米工艺研究", 《粮食与油脂》, no. 7, 31 December 2012 (2012-12-31) *
文镜,等: "功能红曲醋的制备及其降血脂功能的研究", 《食品科学》, vol. 28, no. 9, 31 December 2007 (2007-12-31) *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105104986A (en) * 2015-07-08 2015-12-02 成都大学 Preparation method of tartary buckwheat red yeast rice
CN105104986B (en) * 2015-07-08 2018-10-02 成都大学 A kind of preparation method of bitter buckwheat red yeast rice
CN105695514B (en) * 2015-08-26 2020-02-18 泸州品创科技有限公司 Red rice red yeast and preparation method and application thereof
CN105695514A (en) * 2015-08-26 2016-06-22 泸州老窖集团养生酒业有限责任公司 Purple rice red yeast and preparation method and application thereof
CN105524792A (en) * 2016-02-03 2016-04-27 浙江红石梁集团济公家酒坊有限公司 Preparation method of mixed distiller's yeast
CN105886315A (en) * 2016-05-12 2016-08-24 山西紫林醋业股份有限公司 Method for brewing super-mature vinegar by adopting red yeast prepared from tartary buckwheat
CN105886315B (en) * 2016-05-12 2019-09-10 山西紫林醋业股份有限公司 The brewing method of bitter buckwheat monascus old mature vinegar
CN108085343A (en) * 2018-01-16 2018-05-29 福建省农业科学院农业工程技术研究所 The production technology of fermented grain functional Monascus powder

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