CN103087893B - Preparation method of composite coarse cereals monascus - Google Patents

Preparation method of composite coarse cereals monascus Download PDF

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CN103087893B
CN103087893B CN201310014061.9A CN201310014061A CN103087893B CN 103087893 B CN103087893 B CN 103087893B CN 201310014061 A CN201310014061 A CN 201310014061A CN 103087893 B CN103087893 B CN 103087893B
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monascus
red
substratum
food
chinese medicine
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CN103087893A (en
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宋春雪
赵红年
曹晋宜
丁静
张敏
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Shanxi Jinlongyu Liangfen vinegar industry Co.,Ltd.
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SHANXI LIANGFEN VINEGAR INDUSTRY Co Ltd
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Abstract

The invention discloses a preparation method of composite coarse cereals monascus, belonging to the field of bioengineering microbial fermentation. The preparation method comprises the following steps of: respectively inoculating and culturing pigmental monascus, ester-producing monascus and functional monascus on a PDA (Potato Dextrose Agar) culture medium; preparing a spore suspension liquid from a bacterial strain inclined culture medium, and inoculating a seed culture fluid into the spore suspension liquid to carry out culturing; inoculating a pigmental monascus liquid seed on a sorghum rice culture medium, inoculating a functional monascus liquid seed on a myotonin culture medium, inoculating a functional monascus liquid seed on a buckwheat culture medium, inoculating an ester-producing monascus liquid seed on a millet culture medium, and respectively culturing in a stacking way to obtain four monascus; and mixing the four monascus to prepare the composite coarse cereals monascus, wherein the mass ratio of sorghum rice monascus to myotonin monascus to buckwheat monascus to millet monascus is 17:30:30:23. With the adoption of the preparation method, contents of monacolin-K, flavonoid matters and the like in a product are increased, so that the health efficacy of reducing blood fat of mature vinegar is strengthened when the flavor and the quality of the mature vinegar are ensured.

Description

A kind of preparation method of composite coarse grains red colouring agent for food, also used as a Chinese medicine
Technical field
The invention belongs to biotechnology microorganism fermentation field, be specifically related to a kind of preparation method of composite coarse grains red colouring agent for food, also used as a Chinese medicine.
Background technology
Monascus can produce amylase, saccharifying enzyme, proteolytic enzyme, monascorubin etc. in yeast making process, in fermenting process, can produce succsinic acid, citric acid and lovastatin isoreactivity material, being applied to vinegar brewing industry can increase the color and luster local flavor of seasonings, improve the quality structure of seasonings, improve product specification, make it have stronger nourishing function.
Red colouring agent for food, also used as a Chinese medicine is that monascus is bred the goods on rice or other food crop, can be used as medicinal and food colorant, China before more than 600 years with regard to producing as food, with a long history.
For a long time, microorganism worker takes much count of research, the utilization to red colouring agent for food, also used as a Chinese medicine both at home and abroad, but mostly concentrates on traditional wine brewing, vinegar processed, tinting material, and about producing the research of the aspects such as succsinic acid, citric acid, and its pharmaceutical use is not subject to due attention.Within 1979, Japan rattan chapter professor far away isolates cholesterol synthesis inhibitor MonacolinK from red monascus Monascusruber, Chinese scholars starts it to carry out the discussion of medicinal biological value, and utilize modern biochemistry technology to its effective physiologically active substance extract, separated, analyze.In recent years, functional factor in monascus (as biologically active substances such as cholesterol inhibitor, hypotensive hypoglycemic material, ergosterol and Substances) and toxin etc., cause just gradually scholars' interest, its pharmaceutical use also obtains people's attention thereupon gradually.
At present, the lipopenicillinase class medicine at home and abroad occurring on market has lovastatin, Simvastatin, Pravastatin, fluvastatin, atorvastatin, Cerivastatin, zhibituo, Xuezhikang etc., and their reducing blood-fat mechanism is all relevant with Monacolin K.Thereby the edible vinegar that utilizes red colouring agent for food, also used as a Chinese medicine exploitation to possess antilipemic healthy effect has a extensive future.
Summary of the invention
The preparation method who the object of this invention is to provide a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine, to reach the object of utilizing red colouring agent for food, also used as a Chinese medicine exploitation to possess the vinegar of antilipemic healthy effect.
Invention thinking of the present invention is: by take sorghum rice, seed of Job's tears, bitter buckwheat, millet, be starter-making materials, inoculate respectively pigmental Monascus, functional Monascus mould, produce ester monascus specie and make single grain red colouring agent for food, also used as a Chinese medicine, carry out composite, thereby make composite coarse grains red colouring agent for food, also used as a Chinese medicine.
The present invention is achieved by the following technical solutions:
A preparation method for composite coarse grains red colouring agent for food, also used as a Chinese medicine, comprises the following steps:
(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivation 5-6 days on the PDA substratum containing 2% glucose, 1% maltose, 2% agar, inoculation is produced ester monascus in 30 ℃ of cultivation 10-12 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;
(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is made to spore suspension with spore under aseptic washing, and the inoculum size access seed culture fluid with 6% is cultivated 48-72h for 32 ℃ on the rotary type shaking table of 200r/min;
Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2o, 0.15%KH 2pO 4, 121 ℃ of sterilizing 15min;
The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2o, lactic acid adjust pH 4.5;
Produce ester monascus liquid seeds nutrient solution: liquid glucose lO obx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2pO 4;
(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3-4h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;
The pigmental Monascus liquid seeds of its quality 10% will be accessed on sorghum rice substratum, on seed of Job's tears substratum, access the mould liquid seeds of functional Monascus of its quality 10%, on buckwheat substratum, access the mould liquid seeds of functional Monascus of its quality 10%, on millet substratum, access the product ester monascus liquid seeds of its quality 10%, difference pile, keep spreading 24-48h out after 36-48h, surface red aspergillus hyphae spot covers with substantially, shakeout, every 6-8h, turn over Qu Yici, and keep moisture, be cultured to 6-15d, can go out bent dry, obtaining water content is tetra-kinds of red colouring agent for food, also used as a Chinese medicine of 10%-12%,
(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, obtains composite coarse grains red colouring agent for food, also used as a Chinese medicine.
Further, to the pigmental Monascus liquid seeds that accesses its quality 10% on the sorghum rice substratum preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steams rice surface and occurs red point, now song material is spread out a little, again after 24h, temperature can rise to 42-45 ℃, steams rice surface red aspergillus hyphae spot and substantially covers with, and now song material is shakeout, every 6-8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 6-7 days, can go out bent dry.
To access on the seed of Job's tears substratum preparing its quality 10% the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 36-38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6-8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 12-15 days, can go out bent dry.
To accessing its quality 10% on the buckwheat substratum preparing) the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 36-38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6-8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 12-15 days, can go out bent dry.
To access on the millet substratum preparing its quality 10% product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 40h, temperature can rise to 40-43 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6-8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 8-10 days, can go out bent dry.
The composite coarse grains red colouring agent for food, also used as a Chinese medicine coupling effect that traditional Daqu and the present invention are made is analyzed and researched:
With reference to traditional Shanxi mature vinegar technique, adding 54.5%(to calculate with staple food grain) the basis of the special-purpose Daqu of Shanxi mature vinegar on, introduce the composite coarse grains red colouring agent for food, also used as a Chinese medicine of 8% (calculating with staple food grain), with song for grain, carry out brewageing of old vinegar, through a large amount of research and tests, having extracted altogether the product of 10 batches analyzes and detects in strict accordance with standard, the mean value of finally getting 10 batch datas is analyzed and is compared with the index of not adding the old vinegar blank product of composite coarse grains red colouring agent for food, also used as a Chinese medicine gained, and result is as table 1:
Table 1
Compared with prior art, in the present invention, introduce the composite coarse grains red colouring agent for food, also used as a Chinese medicine that functional Monascus mould species makes, improved the content of Monacolin K, Flavonoid substances etc. in product, when guaranteeing the local flavor and quality of old vinegar, the health-care effect of having strengthened old vinegar reducing blood-fat, has wide market outlook.
Accompanying drawing explanation
Fig. 1 is FB(flow block) of the present invention.
Embodiment
Embodiment 1
A preparation method for composite coarse grains red colouring agent for food, also used as a Chinese medicine, comprises the following steps:
(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 5 days on the PDA substratum containing 2% glucose, 1% maltose, 2% agar, inoculation is produced ester monascus in 30 ℃ of cultivations 11 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;
(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is made to spore suspension with spore under aseptic washing, and the inoculum size access seed culture fluid with 6% is cultivated 48h for 32 ℃ on the rotary type shaking table of 200r/min;
Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2o, 0.15%KH 2pO 4, 121 ℃ of sterilizing 15min;
The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2o, lactic acid adjust pH 4.5;
Produce ester monascus liquid seeds nutrient solution: liquid glucose lO obx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2pO 4;
(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;
To the pigmental Monascus liquid seeds that accesses its quality 10% on the sorghum rice substratum preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 24h, temperature can rise to 42 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 7h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 6 days, can go out bent dry;
To access on the seed of Job's tears substratum preparing its quality 10% the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 37 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 12 days, can go out bent dry;
To the mould liquid seeds of functional Monascus that accesses its quality 10% on the buckwheat substratum preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 36 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 7h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 13 days, can go out bent dry;
To access on the millet substratum preparing its quality 10% product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 40h, temperature can rise to 40 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 10 days, can go out bent dry;
(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, obtains composite coarse grains red colouring agent for food, also used as a Chinese medicine.
Embodiment 2
A preparation method for composite coarse grains red colouring agent for food, also used as a Chinese medicine, comprises the following steps:
(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 5.5 days on the PDA substratum containing 2% glucose, 1% maltose, 2% agar, inoculation is produced ester monascus in 30 ℃ of cultivations 12 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;
(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is made to spore suspension with spore under aseptic washing, and the inoculum size access seed culture fluid with 6% is cultivated 55h for 32 ℃ on the rotary type shaking table of 200r/min;
Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2o, 0.15%KH 2pO 4, 121 ℃ of sterilizing 15min;
The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2o, lactic acid adjust pH 4.5;
Produce ester monascus liquid seeds nutrient solution: liquid glucose lO obx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2pO 4;
(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3.5h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;
To the pigmental Monascus liquid seeds that accesses its quality 10% on the sorghum rice substratum preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 24h, temperature can rise to 43 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 6.5 days, can go out bent dry;
To access on the seed of Job's tears substratum preparing its quality 10% the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 13 days, can go out bent dry;
To the mould liquid seeds of functional Monascus that accesses its quality 10% on the buckwheat substratum preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 15 days, can go out bent dry;
To access on the millet substratum preparing its quality 10% product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 40h, temperature can rise to 41 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 9 days, can go out bent dry;
(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, obtains composite coarse grains red colouring agent for food, also used as a Chinese medicine.
Embodiment 3
A preparation method for composite coarse grains red colouring agent for food, also used as a Chinese medicine, comprises the following steps:
(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 6 days on the PDA substratum containing 2% glucose, 1% maltose, 2% agar, inoculation is produced ester monascus in 30 ℃ of cultivations 10 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;
(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is made to spore suspension with spore under aseptic washing, and the inoculum size access seed culture fluid with 6% is cultivated 72h for 32 ℃ on the rotary type shaking table of 200r/min;
Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2o, 0.15%KH 2pO 4, 121 ℃ of sterilizing 15min;
The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2o, lactic acid adjust pH 4.5;
Produce ester monascus liquid seeds nutrient solution: liquid glucose lO obx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2pO 4;
(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 4h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;
To the pigmental Monascus liquid seeds that accesses its quality 10% on the sorghum rice substratum preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 24h, temperature can rise to 44 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 7 days, can go out bent dry;
To access on the seed of Job's tears substratum preparing its quality 10% the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 36 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 7h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 15 days, can go out bent dry;
To the mould liquid seeds of functional Monascus that accesses its quality 10% on the buckwheat substratum preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 37 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 12 days, can go out bent dry;
To access on the millet substratum preparing its quality 10% product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 40h, temperature can rise to 43 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 7h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 8 days, can go out bent dry;
(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, obtains composite coarse grains red colouring agent for food, also used as a Chinese medicine.
Embodiment 4
A preparation method for composite coarse grains red colouring agent for food, also used as a Chinese medicine, comprises the following steps:
(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivations 6 days on the PDA substratum containing 2% glucose, 1% maltose, 2% agar, inoculation is produced ester monascus in 30 ℃ of cultivations 12 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;
(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is made to spore suspension with spore under aseptic washing, and the inoculum size access seed culture fluid with 6% is cultivated 60h for 32 ℃ on the rotary type shaking table of 200r/min;
Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2o, 0.15%KH 2pO 4, 121 ℃ of sterilizing 15min;
The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2o, lactic acid adjust pH 4.5;
Produce ester monascus liquid seeds nutrient solution: liquid glucose lO obx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2pO 4;
(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;
To the pigmental Monascus liquid seeds that accesses its quality 10% on the sorghum rice substratum preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 24h, temperature can rise to 45 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 7h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 6.5 days, can go out bent dry;
To access on the seed of Job's tears substratum preparing its quality 10% the mould liquid seeds of functional Monascus, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 37 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 7h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 14 days, can go out bent dry;
To the mould liquid seeds of functional Monascus that accesses its quality 10% on the buckwheat substratum preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 37 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 14 days, can go out bent dry;
Mix and connect the sort of quyi: to access on the millet substratum preparing its quality 10% product ester monascus liquid seeds, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 40h, temperature can rise to 42 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 10 days, can go out bent dry;
(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, obtains composite coarse grains red colouring agent for food, also used as a Chinese medicine.

Claims (5)

1. a preparation method for composite coarse grains red colouring agent for food, also used as a Chinese medicine, is characterized in that, comprises the following steps:
(1) inclined plane inoculating: inoculate respectively pigmental Monascus in 30 ℃ of cultivation 5-6 days on the PDA substratum containing 2% glucose, 1% maltose, 2% agar, inoculation is produced ester monascus in 30 ℃ of cultivation 10-12 days, and functional Monascus is mould, cultivates 7 days in 30 ℃;
(2) preparation of liquid seeds: the bacterial strain slant medium of large volume production spore is made to spore suspension with spore under aseptic washing, and the inoculum size access seed culture fluid with 6% is cultivated 48-72h for 32 ℃ on the rotary type shaking table of 200r/min;
Pigmental Monascus liquid seeds nutrient solution wherein: rice juice, 0.4% NaNO 3, 0.1%MgSO 47H 2o, 0.15%KH 2pO 4, 121 ℃ of sterilizing 15min;
The mould liquid seeds nutrient solution of functional Monascus: glucose 6%, peptone 2.5%, yeast powder 0.5%, 0.2% NaNO 3, 0.1%MgSO 47H 2o, lactic acid adjust pH 4.5;
Produce ester monascus liquid seeds nutrient solution: liquid glucose l0 obx, 0.2%NaN0 3, 0.1%MgS0 4, 0.25%KH 2pO 4;
(3) solid-state cultivation: after sorghum rice, seed of Job's tears, buckwheat, millet are cleaned with clear water respectively, soak 3-4h, drain, meal braizing machine steamed rice 50min, airing is cooling, makes respectively sorghum rice substratum, seed of Job's tears substratum, buckwheat substratum, millet substratum;
The pigmental Monascus liquid seeds of its quality 10% will be accessed on sorghum rice substratum, on seed of Job's tears substratum, access the mould liquid seeds of functional Monascus of its quality 10%, on buckwheat substratum, access the mould liquid seeds of functional Monascus of its quality 10%, on millet substratum, access the product ester monascus liquid seeds of its quality 10%, difference pile, keep spreading 24-48h out after 36-48h, surface red aspergillus hyphae spot covers with substantially, shakeout, every 6-8h, turn over Qu Yici, and keep moisture, be cultured to 6-15 days, can go out bent dry, obtaining water content is tetra-kinds of red colouring agent for food, also used as a Chinese medicine of 10%-12%,
(4) mix: above-mentioned four kinds of red colouring agent for food, also used as a Chinese medicine are mixed, wherein sorghum rice red colouring agent for food, also used as a Chinese medicine: seed of Job's tears red colouring agent for food, also used as a Chinese medicine: buckwheat red colouring agent for food, also used as a Chinese medicine: the mass ratio of millet red koji is 17:30:30:23, obtains composite coarse grains red colouring agent for food, also used as a Chinese medicine.
2. the preparation method of a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine according to claim 1, it is characterized in that, to the pigmental Monascus liquid seeds that accesses its quality 10% on the sorghum rice substratum preparing, pile, after 36h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 24h, temperature can rise to 42-45 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6-8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 6-7 days, can go out bent dry.
3. the preparation method of a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine according to claim 1 and 2, it is characterized in that, to the mould liquid seeds of functional Monascus that accesses its quality 10% on the seed of Job's tears substratum preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 36-38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6-8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 12-15 days, can go out bent dry.
4. the preparation method of a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine according to claim 1 and 2, it is characterized in that, to the mould liquid seeds of functional Monascus that accesses its quality 10% on the buckwheat substratum preparing, pile, after 48h, bent material temperature degree can rise to 38 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 48h, temperature can rise to 36-38 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6-8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 12-15 days, can go out bent dry.
5. the preparation method of a kind of composite coarse grains red colouring agent for food, also used as a Chinese medicine according to claim 1 and 2, it is characterized in that, to the product ester monascus liquid seeds that accesses its quality 10% on the millet substratum preparing, pile, after 48h, bent material temperature degree can rise to 42 ℃, steam rice surface and occur red point, now song material is spread out a little, then after 40h, temperature can rise to 40-43 ℃, steaming rice surface red aspergillus hyphae spot covers with substantially, now song material is shakeout, every 6-8h, turn over Qu Yici, with atomizer, carry out appropriate moisturizing, be cultured to 8-10 days, can go out bent dry.
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