CN105532266A - Termitomyces albuminosus cultivation method - Google Patents
Termitomyces albuminosus cultivation method Download PDFInfo
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Abstract
The invention provides a termitomyces albuminosus cultivation method. The method includes the steps of spawn runing cultivation, wherein fungus bags are sent to a cultivation chamber, the temperature of the cultivation chamber is controlled to maintain at 20-25 DEG C, and relative humidity is controlled to be 55-65%; soil covering, wherein after the fungus bags are transferred into a fruiting house, fungus sacks of the fungus bags are removed, fungus sticks are taken out, the fungus sticks which are taken out are well placed, tightly covered with soil and then completely watered; finally the fungus sticks are covered with a layer of turfy soil which is 2-4 cm in thickness, and the soil PH is maintained at 5.5-7; fruiting management, wherein before fruiting, the temperature in the fruiting house is controlled at 20-30 DEG C, and the temperature difference between day and night is controlled at 8-10 DEG C. The termitomyces albuminosus cultivation method has the advantages that the wild living environment of termitomyces albuminosus is simulated on the aspects of temperature, temperature difference between day and night and soil, the quality of produced termitomyces albuminosus can be quite similar to that of wild termitomyces albuminosus, the production cycle of the termitomyces albuminosus is shortened by about 90 days, and economic profits of industrialization of the termitomyces albuminosus are raised.
Description
Technical field
The invention belongs to edible mushroom technical field, is more particularly a kind of cultivation method of collybia albuminosa.
Background technology
Collybia albuminosa is commonly called as " hat in bacterium ".Collybia albuminosa meat is thick big and fleshy, and matter filament is white, the fresh and sweet delicious and crisp of taste.Containing the necessary amino acid of human body, protein, fat, also containing the material such as various vitamin and calcium, phosphorus, core yellow acid.Some areas in collybia albuminosa only southwest, a few province in the southeast and Taiwan produce, and because of its wild yielding poorly, price is higher.Collybia albuminosa is not only has abundant nutritive value, also there is very high medical value simultaneously, there is beneficial stomach, clear god, control the effect such as hemorrhoid and reducing blood lipid, also there is the effect such as enriching the blood to restore normal menstruation, strengthening the spleen and stomach, can be used for treatment poor appetite, endless diarrhea, all diseases of blood under hemorrhoid, is one of traditional medicinal fungi of China.
Because the growth mechanism of collybia albuminosa and most wild mushrooms has huge difference, collybia albuminosa and termite seek commensalism, the secretion collybia albuminosa that have left termite is just difficult to existence, and both mutual reciprocity and mutual benefit, this kind of ecotope causes the particular/special requirement of collybia albuminosa to growth conditions.Due to this particular/special requirement to environment of collybia albuminosa, make its artificial cultivation technique be the Focal point and difficult point studied always.
Summary of the invention
For overcoming the above problems, the invention provides the wild living environment of a kind of simulation collybia albuminosa, making product quality and wild product quality collybia albuminosa cultivation method closely;
The cultivation method of collybia albuminosa of the present invention comprises the steps:
(1). send out bacterium and cultivate: send bacterium bag to culturing room, control culturing room's temperature and remain on 20--25 degree Celsius, relative moisture controls at 55%--65%, and timing ventilation, after mycelia covers with bacterium bag, proceeded to bacterium bag in mushroom room and carried out next step every day;
(2). earthing: after proceeding to mushroom room, the bacterium bag of bacterium bag is removed, takes out bacterium rod, the bacterium rod of taking-up is put well, above earthing being covered tightly, then irrigate with water; The last turfy soil covering one deck 2-4 cm thick again, makes P in soil H remain on 5.5--7, enters the management of producing mushroom stage;
(3). management of producing mushroom: before fruiting, the temperature in mushroom producing room controls at 20--30 degree Celsius, and day and night temperature controls at 8-10 degrees Celsius, and air humidity controls at 90%--95%, and intensity of illumination controls in 250-300 luxs; After fruiting, the temperature in mushroom producing room remains on 20-30 degrees Celsius, and day and night temperature remains on 8-10 degree Celsius, and air humidity controls at 85%--95%, and illumination controls, in 100--300 lux, namely to enter harvest stages;
(4). gather: can gather when bacteria cover diameter reaches 2-5 centimetre.
Further, the making step of described bacterium bag comprises: be inoculated into by liquid strain in inoculation bag under sterile conditions; Described inoculation bag comprises composts or fertilisers of cultivating and the bacterium bag of composts or fertilisers of cultivating is housed.
Further, the making step of described inoculation bag is:
(1). composts or fertilisers of cultivating: raw material percentage being respectively 45% cotton seed hull, 22% wheat bran, 18% wood chip, 7% corncob, 5% corn flour and 3% dregs of beans adds water stirring in agitator, and water content control is at 60%--65%;
(2). pack: the composts or fertilisers of cultivating be stirred is loaded in bacterium bag, seals with the vinyl cover of air-permeable dampproof;
(3). sterilizing: will the bacterium bag of mouth be sealed, and transfer in high-temperature sterilizing chambers, carry out sterilization treatment.
Further, the making step of liquid strain is:
(1). preparation liquid culture medium: first potato is boiled rear extraction supernatant, the then solution of preparation containing 3% glucose, 1% corn flour, 0.1% magnesium sulfate and 0.15% potassium dihydrogen phosphate, by potato supernatant and the mixing of above-mentioned solution;
(2). shake flask is cultivated: after being prepared by liquid nutrient medium, loads in conical flask, and adds little bead, and bottleneck tampon, brown paper seal, sterilizing 30 minutes under 1.5 thousand grams/cm of pressure; Then drop into slant strains, be placed in 23--25 degree Celsius of lower quiescent culture 24 hours; When mycelium germination, be placed on shaken cultivation on reciprocal shaker again, frequency of oscillation is 80 ~ 100 times per minute, and amplitude is 6 ~ 10 centimetres; The temperature of shaking table room controls at 24 DEG C ~ 25 DEG C, and incubation time is 4-6 days.
Further, the making step of described liquid strain is:
(1). preparation liquid culture medium: first potato is boiled rear extraction supernatant, the then solution of preparation containing 3% glucose, 1% corn flour, 0.1% magnesium sulfate and 0.15% potassium dihydrogen phosphate, by potato supernatant and the mixing of above-mentioned solution;
(2). shake flask is cultivated: loading capacity is in the conical flask of 500 milliliters, often bottled enter 100 milliliters, and add little bead, bottleneck tampon, brown paper seal, sterilizing 30 minutes under 1.5 thousand grams/cm of pressure; Then the slant strains dropped into, quiescent culture 24 hours at being placed in 23 DEG C ~ 25 DEG C; When mycelium germination, be placed on shaken cultivation on reciprocal shaker again, frequency of oscillation is 80 ~ 100 times per minute, and amplitude is 6 ~ 10 centimetres; The temperature of shaking table room controls at 24 DEG C ~ 25 DEG C, incubation time 4-6 days;
(3). liquid seeds tank ferments: under the protection of pyrosphere; pour rapidly the bacterial classification in above-mentioned steps (2) into strain cultivation tank; close tank mouth to cultivate; aseptically breather pipe is installed; pressure in tank is made at 0.02MPa-0.04MPa, after passing into appropriate oxygen with oxygen therapy machine, to cultivate 48-72 hour.
Further, fill the solution that content is 3% corn flour, 3% glucose, 2% wheat bran, 0.3% peptone, 0.1% magnesium sulfate, 0.15% potassium dihydrogen phosphate, 0.05% Cobastab and 0.3% sodium nitrate in described strain cultivation tank, the pH value of this solution is 6.
Further, described inoculating process comprises and being wrapped by inoculating gun multilayer gauze, outsourcing one deck brown paper, tiredly ties; Inoculating gun and inoculated tube are put into autoclave, sterilizing 40 minutes; By the inoculated tube after sterilizing, under the protection of alcolhol burner flame, receive fast on strain cultivation tank inoculation valve, open inoculation valve, sterilize under inoculating gun being placed on the flame of alcolhol burner, then with inoculation in inoculating gun past inoculation bag.
Beneficial effect of the present invention is the wild living environment from temperature and day and night temperature and soil aspect simulation collybia albuminosa, make the product quality produced and wild quality quite similar, and make the production cycle of product shorten about 90 days, the economic benefit of the product industrialization of raising.
Accompanying drawing explanation
Accompanying drawing 1 is process chart of the present invention.
Embodiment
By reference to the accompanying drawings 1 and below embodiment illustrate content of the present invention further, so that the public grasps implementation method of the present invention better; Concrete technology step is:
1, the making of bag is inoculated:
Selected raw material be fresh, without the quality raw materials gone mouldy, meet the every nutritional requirement of Termitomyces albuminosus with black skin production technology.Through every technology for detection, all meet fruiting production requirement.
(1). composts or fertilisers of cultivating: the percentage of each raw material is respectively: 45% cotton seed hull, 22% wheat bran, 18% wood chip, 7% corncob, 5% corn flour, 3% dregs of beans; To detect qualified raw material, require to add transit mixer according to said ratio, add water and stir, water content control is at about 60%-63%.
(2). pack: by the composts or fertilisers of cultivating be stirred, by automatic transmission band, deliver to automatic packer place, bacterium bag is enclosed within automatic packer that (bacterium bag can select polyethylene plastic bag or polypropylene plastics pocket by operating personnel, select wide 17 centimetres, long 33 centimetres, thick 0.05 millimeter), install each bacterium bag of rear weighing, guarantee that each bacterium bag is at about 1.2-1.4 kilogram; The vinyl cover sealing of operating personnel's air-permeable dampproof.
(3). sterilizing: will the bacterium bag of mouth be sealed, and transfer in high-temperature sterilizing chambers, carry out sterilization treatment; By packed for bacterium enter after high-temperature sterilizing chambers, high-temperature sterilizing chambers is completely closed, and then start heating, in heating after 3 hours, make the pressure of sterilizing chamber reach 0.15Mpa, just passable when temperature reaches 121 degrees Celsius; Do not open autoclave after having heated at once, wait for and opening again after 30 minutes, just can be vaccinated with after cooling.
2, liquid strain makes:
(1). obtaining liq medium: first potato is boiled extraction supernatant, the then solution of preparation containing 3% glucose, 1% corn flour, 0.1% magnesium sulfate and 0.15% potassium dihydrogen phosphate, finally mix potato supernatant and above-mentioned solution and water.This liquid culture medium is applicable to the cultivation of multiple eating bacterium bacterial classification.
(2). shake flask is cultivated: after liquid culture medium prepares, loading capacity is in the conical flask of 500 milliliters, often bottled enter 100 milliliters, and add little bead, bottleneck tampon, brown paper seal, sterilizing 30 minutes under 1.5 thousand grams/cm of pressure; Then the slant strains of a piece about 2 square centimeters is dropped into, quiescent culture 24 hours at 23 DEG C ~ 25 DEG C; When mycelium germination, be placed on shaken cultivation on reciprocal shaker again, frequency of oscillation is 80 ~ 100 times per minute, and amplitude is 6 ~ 10 centimetres; The temperature of shaking table room controls at 24 DEG C ~ 25 DEG C, and incubation time is generally at about 4-6 days.The bacterial classification that this step is cultivated can directly be inoculated, and also can enter next step and again cultivate.
(3). liquid seeds tank ferments: under the protection of pyrosphere, pour rapidly the bacterial classification that step (2) is cultivated into strain cultivation tank, closes tank mouth and cultivates; Aseptically breather pipe is installed, makes pressure in tank at 0.02MPa-0.04MPa, after passing into appropriate oxygen with oxygen therapy machine, cultivate 48-72 hour.Cultivate the standard terminated: culture fluid is as clear as crystal, wherein left floating a large amount of little mycelium pellet, and with the distinctive fragrant of mushroom class.
Medium in strain cultivation tank is the solution containing 3% corn flour, 3% glucose, 2% wheat bran, 0.3% peptone, 0.1% magnesium sulfate, 0.15% potassium dihydrogen phosphate, 0.05% Cobastab, 0.3% sodium nitrate, and the pH value of this solution is 6.
Before strain cultivation tank carries out new bacterial classification production, with the clear water of flowing, repeatedly must wash away the inwall of tank, with specific long handle iron wire brush, brush away the mycoderma on tank skin, the dirts such as feed liquid.To the tank mouth pad of tank, inoculation valve, intake valve, each several part parts such as switch board check, if there is fault, need to get rid of in time.First add water the midline of visor, and fastening inoculation lid, closes intake valve, opening starting switch, by adding hot key, entering sterilizing state, and when temperature reaches 100 degrees Celsius, continue 20 minutes, sterilization terminates, and water is bled off.
3, inoculate
Inoculation in the aseptic inoculation, and staff will wear the work clothes through sterilization, is wrapped by inoculating gun multilayer gauze, outsourcing one deck brown paper, is tying with cotton rope etc. is tired; Inoculating gun and inoculated tube are put into autoclave, pot cover is built, sterilizing 40 minutes; By the inoculated tube after sterilizing, under the protection of alcolhol burner flame, receive on strain cultivation tank inoculation valve fast; open inoculation valve; sterilize under inoculating gun being placed on the flame of alcolhol burner, allow rear inoculating gun toward inoculation in the inoculation bag cooled, every bag of inoculum concentration is at 35-40ml.
4, send out bacterium to cultivate
The bacterium bag connecting bacterial classification is entered culturing room through conveyer belt; According to entry time, neatly put on the top of the shelf, carry out warehouse-in record; Cultivate indoor temperature and remain on 20-25 degree Celsius, relative moisture is at 55%--65%.Every day, timing ventilation, sooner or later respectively once, was no more than half an hour at every turn; Between bacteria developing period, administrative staff make regular check on culture bag, if the bacterium bag finding that there is miscellaneous bacteria infection will clean out culturing room, in time in case infect other bacterium bag; After mycelia covers with bacterium bag, bacterium bag is proceeded in mushroom room.
5, added earthing
After proceeding to, the bacterium bag of bacterium bag is removed, bacterium rod take out, by bacterium rod erect be placed in fruiting shelf, bacterium rod with bacterium rod be spaced apart 5 centimetres, above earthing, until covered tightly by bacterium rod, thickness of earth covering is about 1-3 centimetre, and the complete rear water of earthing irrigates; Finally, then the turfy soil being covered with one deck 2-4 cm thick makes the pH value of soil remain between 5.5-7, and turfy soil has the effect of ventilative water suction, and turfy soil is for simulating the field soil of collybia albuminosa.In order to better utilize space, fruiting shelf adopts angle bar to make, and width is: middle 140cm, by the 70cm of wall, is highly 70cm, specifically can according to developed width, highly making.
6, management of producing mushroom
Before fruiting, fruiting indoor temperature controls between 20-30 degree Celsius, day and night temperature controls at 8--10 degree Celsius, material temperature controls between 25-27 degree Celsius, air humidity remains on about 90%, use grey light belt, give the light scattering that collybia albuminosa is certain, intensity of illumination controls about 300 luxs.Timing ventilation, keeps with fresh air in mushroom producing room.
After fruiting, strengthen management, improve output, promote quality.The mushroom producing room temperature in fruiting period will remain on 20-30 degree Celsius, and day and night temperature remains on 8--10 degree Celsius, under the thermal stimulation of 8-10 degree Celsius, be conducive to the quick differentiation of fruit body, material temperature controls at 25 degrees centigrade, and air humidity controls, at 85%-95%, to add forced ventilation.During fruiting, collybia albuminosa needs certain astigmatism to irradiate, and intensity of illumination should control in 100-300 lux.
7, gather
Fruiting about 5 days, when bacteria cover diameter grows to 2-5 centimetre, first batch of mushroom of can gathering; Gather and want in time, to gather before upper cap does not open completely; Can gather altogether about three batches of mushrooms, about 10 days, the interval of every batch of mushroom.Hold stem when gathering, gently turn-knob, connect root and together pull up, put into basket of gathering.Collybia albuminosa also will process bed surface after having gathered, and after every batch of mushroom has been adopted, the mushroom root that bed surface stays and dead mushroom all should be cleaned out in time, in order to avoid cause rotten, cause miscellaneous bacteria to infect.Supplement moistening fine earth in time, keep bed surface smooth.
The collybia albuminosa of the present invention's cultivation and the nutrient composition content of wild collybia albuminosa contrast as follows:
The collybia albuminosa that the present invention produces: every 100 grams moisture 92.61%, dry matter 7.39%;
Wild collybia albuminosa: every 100 grams of fresh collybia albuminosas moisture 92.43%, dry matter 7.57%;
Wherein: the composition of dry matter is as follows:
The wild collybia albuminosa of collybia albuminosa that the present invention produces
Containing crude protein 34.94%; Containing crude protein 32.58%;
Crude fat 5.40%; Crude fat 5.75%;
Raw fiber 14.91%; Raw fiber 15.11%;
Soluble sugar 4.5%; Soluble sugar 4.1%;
Hydrolysis sugar 9.59%; Hydrolysis sugar 8.73%;
Ash content 7.73%; Ash content 7.93%;
All contain ergot and stay alcohol and 16 seed amino acids and vitamin C.
Can find out from above-mentioned data, the collybia albuminosa of the present invention's cultivation is compared with wild collybia albuminosa, and its nutritions components has met or exceeded wild collybia albuminosa.
Claims (7)
1. a collybia albuminosa cultivation method, is characterized in that comprising the steps:
(1). send out bacterium and cultivate: send bacterium bag to culturing room, control culturing room's temperature and remain on 20--25 degree Celsius, relative moisture controls at 55%--65%, and timing ventilation, after mycelia covers with bacterium bag, proceeded to bacterium bag in mushroom room and carried out next step every day;
(2). earthing: after proceeding to mushroom room, the bacterium bag of bacterium bag is removed, takes out bacterium rod, the bacterium rod of taking-up is put well, above earthing being covered tightly, then irrigate with water; The last turfy soil covering one deck 2-4 cm thick again, makes P in soil H remain on 5.5-7, enters the management of producing mushroom stage;
(3). management of producing mushroom: before fruiting, the temperature in mushroom producing room controls at 20--30 degree Celsius, and day and night temperature controls at 8-10 degrees Celsius, and air humidity controls at 90%--95%, and intensity of illumination controls in 250-300 luxs; After fruiting, the temperature in mushroom producing room remains on 20-30 degrees Celsius, and day and night temperature remains on 8-10 degree Celsius, and air humidity controls at 85%--95%, and illumination controls, in 100--300 lux, namely to enter harvest stages;
(4). gather: when bacteria cover diameter reaches 2-5 centimetre, can gather.
2. collybia albuminosa cultivation method according to claim 1, is characterized in that the making step of described bacterium bag comprises: be inoculated into by liquid strain in inoculation bag under sterile conditions; Described inoculation bag comprises composts or fertilisers of cultivating and the bacterium bag of composts or fertilisers of cultivating is housed.
3. collybia albuminosa cultivation method according to claim 2, is characterized in that the making step of described inoculation bag is:
(1). composts or fertilisers of cultivating: raw material percentage being respectively 45% cotton seed hull, 22% wheat bran, 18% wood chip, 7% corncob, 5% corn flour and 3% dregs of beans adds water stirring in agitator, and water content control is at 60%--65%;
(2). pack: the composts or fertilisers of cultivating be stirred is loaded in bacterium bag, seals with the vinyl cover of air-permeable dampproof;
(3). sterilizing: will the bacterium bag of mouth be sealed, and transfer in high-temperature sterilizing chambers, carry out sterilization treatment.
4. collybia albuminosa cultivation method according to claim 2, is characterized in that the making step of liquid strain is:
(1). preparation liquid culture medium: first potato is boiled rear extraction supernatant, the then solution of preparation containing 3% glucose, 1% corn flour, 0.1% magnesium sulfate and 0.15% potassium dihydrogen phosphate, by potato supernatant and the mixing of above-mentioned solution;
(2). shake flask is cultivated: after being prepared by liquid nutrient medium, loads in conical flask, and adds little bead, and bottleneck tampon, brown paper seal, sterilizing 30 minutes under 1.5 thousand grams/cm of pressure; Then drop into slant strains, be placed in 23--25 degree Celsius of lower quiescent culture 24 hours; When mycelium germination, be placed on shaken cultivation on reciprocal shaker again, frequency of oscillation is 80 ~ 100 times per minute, and amplitude is 6 ~ 10 centimetres; The temperature of shaking table room controls at 24 DEG C ~ 25 DEG C, and incubation time is 4-6 days.
5. collybia albuminosa cultivation method according to claim 2, is characterized in that the making step of liquid strain is:
(1). preparation liquid culture medium: first potato is boiled rear extraction supernatant, the then solution of preparation containing 3% glucose, 1% corn flour, 0.1% magnesium sulfate and 0.15% potassium dihydrogen phosphate, by potato supernatant and the mixing of above-mentioned solution;
(2). shake flask is cultivated: loading capacity is in the conical flask of 500 milliliters, often bottled enter 100 milliliters, and add little bead, bottleneck tampon, brown paper seal, sterilizing 30 minutes under 1.5 thousand grams/cm of pressure; Then the slant strains dropped into, quiescent culture 24 hours at being placed in 23 DEG C ~ 25 DEG C; When mycelium germination, be placed on shaken cultivation on reciprocal shaker again, frequency of oscillation is 80 ~ 100 times per minute, and amplitude is 6 ~ 10 centimetres; The temperature of shaking table room controls at 24 DEG C ~ 25 DEG C, incubation time 4-6 days;
(3). liquid seeds tank ferments: under the protection of pyrosphere; pour rapidly the bacterial classification in above-mentioned steps (2) into strain cultivation tank; close tank mouth to cultivate; aseptically breather pipe is installed; pressure in tank is made at 0.02MPa-0.04MPa, after passing into appropriate oxygen with oxygen therapy machine, to cultivate 48-72 hour.
6. collybia albuminosa cultivation method according to claim 5, it is characterized in that filling the solution that content is 3% corn flour, 3% glucose, 2% wheat bran, 0.3% peptone, 0.1% magnesium sulfate, 0.15% potassium dihydrogen phosphate, 0.05% Cobastab and 0.3% sodium nitrate in described strain cultivation tank, the pH value of this solution is 6.
7. collybia albuminosa cultivation method according to claim 2, is characterized in that described inoculating process comprises and is wrapped by inoculating gun multilayer gauze, outsourcing one deck brown paper, is stranded and ties; Inoculating gun and inoculated tube are put into autoclave, sterilizing 40 minutes; By the inoculated tube after sterilizing, under the protection of alcolhol burner flame, receive fast on strain cultivation tank inoculation valve, open inoculation valve, sterilize under inoculating gun being placed on the flame of alcolhol burner, then with inoculation in inoculating gun past inoculation bag.
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| CN106069188A (en) * | 2016-06-20 | 2016-11-09 | 马碧勇 | A kind of artificial cultivation method of termitomyces |
| CN106718064A (en) * | 2016-12-22 | 2017-05-31 | 普洱滇洪俊生物科技开发有限公司 | The method that dew chicken fir is cultivated using idle tobacco flue-curing house |
| CN107258327A (en) * | 2017-07-31 | 2017-10-20 | 安龙县农望种植农民专业合作社 | A kind of cultural method of collybia albuminosa |
| CN107484554A (en) * | 2017-09-29 | 2017-12-19 | 贵州棒棒食用菌产业有限公司 | A kind of artificial collybia albuminosa cultural method |
| CN108967038A (en) * | 2018-08-28 | 2018-12-11 | 铜陵盛牛菌业有限责任公司 | A kind of method of liquid strain cultivation mushroom |
| CN110122188A (en) * | 2019-06-28 | 2019-08-16 | 广西壮族自治区农业科学院微生物研究所 | Edible mushroom cultivation nutrition promotor and its application |
| CN110637676A (en) * | 2019-10-25 | 2020-01-03 | 葫芦岛农函大玄宇食用菌野驯繁育有限公司 | Termitomyces albuminosus cultivation method and cultivation medium |
| CN111919660A (en) * | 2020-08-11 | 2020-11-13 | 南京康之春生物科技有限公司 | Cultivation method of termitomyces albuminosus |
| CN114902911A (en) * | 2022-04-27 | 2022-08-16 | 山东省农业科学院 | High-density short-period high-yield synergistic fruiting method for long-rooted mushroom sticks |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106069188A (en) * | 2016-06-20 | 2016-11-09 | 马碧勇 | A kind of artificial cultivation method of termitomyces |
| CN106718064A (en) * | 2016-12-22 | 2017-05-31 | 普洱滇洪俊生物科技开发有限公司 | The method that dew chicken fir is cultivated using idle tobacco flue-curing house |
| CN107258327A (en) * | 2017-07-31 | 2017-10-20 | 安龙县农望种植农民专业合作社 | A kind of cultural method of collybia albuminosa |
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| CN110637676A (en) * | 2019-10-25 | 2020-01-03 | 葫芦岛农函大玄宇食用菌野驯繁育有限公司 | Termitomyces albuminosus cultivation method and cultivation medium |
| CN111919660A (en) * | 2020-08-11 | 2020-11-13 | 南京康之春生物科技有限公司 | Cultivation method of termitomyces albuminosus |
| CN114902911A (en) * | 2022-04-27 | 2022-08-16 | 山东省农业科学院 | High-density short-period high-yield synergistic fruiting method for long-rooted mushroom sticks |
| CN114902911B (en) * | 2022-04-27 | 2023-09-05 | 山东省农业科学院 | Fruiting method for high-density short-period high-yield synergy of long-root mushroom sticks |
| CN116195470A (en) * | 2022-05-19 | 2023-06-02 | 北京市农林科学院 | Method for cultivating fruiting of oospore oudemansiella radiata and application thereof |
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