CN105684727A - Culture medium and cultivation method for cultivating black termitomyces albuminosus with enzyme bacterium residues as main raw material - Google Patents
Culture medium and cultivation method for cultivating black termitomyces albuminosus with enzyme bacterium residues as main raw material Download PDFInfo
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- CN105684727A CN105684727A CN201610046901.3A CN201610046901A CN105684727A CN 105684727 A CN105684727 A CN 105684727A CN 201610046901 A CN201610046901 A CN 201610046901A CN 105684727 A CN105684727 A CN 105684727A
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- 108090000790 Enzymes Proteins 0.000 title abstract description 9
- 241001327916 Termitomyces albuminosus Species 0.000 title abstract 5
- 238000012364 cultivation method Methods 0.000 title abstract 3
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 8
- 239000002023 wood Substances 0.000 claims description 8
- 239000004033 plastic Substances 0.000 claims description 7
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- 238000003756 stirring Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
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- 239000002609 medium Substances 0.000 claims description 4
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- 239000011121 hardwood Substances 0.000 claims description 3
- 241000465865 Geodermatophilaceae bacterium Species 0.000 claims description 2
- 230000032683 aging Effects 0.000 claims description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 2
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- 238000007599 discharging Methods 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 238000011049 filling Methods 0.000 claims description 2
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- 230000000694 effects Effects 0.000 description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 description 2
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- 235000019691 monocalcium phosphate Nutrition 0.000 description 2
- 230000005070 ripening Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000222680 Collybia Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
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- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000254109 Tenebrio molitor Species 0.000 description 1
- 240000006677 Vicia faba Species 0.000 description 1
- 235000010749 Vicia faba Nutrition 0.000 description 1
- 235000002098 Vicia faba var. major Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000004596 appetite loss Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
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- 229910052757 nitrogen Inorganic materials 0.000 description 1
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- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention belongs to the technical field of the edible fungi cultivation and particularly relates to a culture medium and a cultivation method for cultivating black termitomyces albuminosus with enzyme bacterium residues as a main raw material. The culture medium is prepared from, by weight, 35-40 parts of enzyme bacterium residues, 20-25 parts of dried Pennisetum giganteum z.x.lin, 20-25 parts of cotton seed hulls, 13-18 parts of wheat bran, 1 part of sugar and 1 part of gypsum. The culture medium solves the problems that according to an existing cultivation method, a lot of cotton seed hulls are used, cost is high, the process is complex, the period is long, and edible fungus crude protein and microelement content is low. The enzyme bacterium residues generated after kitchen waste is manufactured into enzyme bacteria serves as the raw material, the cotton seed hulls and saw dust are properly added as main materials, black termitomyces albuminosus fruiting bags are cultivated, and the enzyme bacterium residues are environmentally friendly and contain various nutritional components which can be used by black termitomyces albuminosus for fruiting. Fruiting products are high in nutrient value and crisp and tender in taste, development of black termitomyces albuminosus and great development of the edible fungus industry are benefited, and the health level of human beings is improved.
Description
Technical field
The invention belongs to fungus growing technique field, specifically a kind of cultivate the culture medium of black termitomyces and cultural method with what ferment dreg was major ingredient。
Background technology
Black termitomyces is a kind of to parasitize a kind of macro fungi extremely special in termitary surrounding soil, and termitomyces color and luster is pure white, fine and tender taste, and delicate fragrance overflows, and mouthfeel is clear and melodious, is famous wild edible fungus。
Black termitomyces is containing aminoacid, protein, fat necessary to human body, possibly together with materials such as various vitamin and calcium, phosphorus, verdoflavin。
The flat sweet in the mouth of black termitomyces, has tonification the intestines and stomach, treatment hemorrhoid hemostasis, stomach reinforcing, clear god, controls the effects such as hemorrhoid, can control the diseases such as insufficiency of the spleen indigestion and loss of appetite, dyspepsia, bleeding hemorrhoids, and often food termitomyces can also improve immunity of organisms, resistance cancerous cell, reduces blood glucose。
Another of black termitomyces is characterized as being phosphorus content height, is the delicacies needing phosphorus supplement personage。
Black termitomyces artificial culture fruiting difficulty is very big; nutritional character in compost is difficult to fruiting when not meeting its characteristic requirements; prior art is cultivated black termitomyces and is mostly that adopting wood flour, cotton seed hull is that major ingredient makes culture medium; large-scale production; there will be woods bacterium contradiction; cost is high, is unfavorable for that sustainable developing environment is protected。
Chinese invention patent CN104396561A, the method disclosing the black termitomyces of a kind of artificial culture, component is pinaster wood flour (or sawdust), the single or any mixture of rubber tree wood flour (or sawdust), parts by weight is 85～92 parts, broad bean seed coat (Testa Viciae fabae) 5～10 parts, bean cake 3～5 parts, adding water and making water content is 50%～70%。
Chinese invention patent CN104705070A discloses the intelligent factory cultivation technique of a kind of black termitomyces, culture medium is hardwood sawdust 68%, wheat bran 20%, tenebrio molitor slurrying 10%, sucrose 1%, Gypsum Fibrosum 1%, loads plastic bag and makes bacterium rod, implant cultivation strain on bacterium rod after sterilizing, utilize Special intelligent automatic control system, growth demand according to black termitomyces, stage by stage complete intelligent automatic control, thus turning out nutritious black Collybia albuminosa (Berk.) Petch mushroom, but the intelligent automatic control system of the method, complicated operation, cost is high, is unfavorable for marketing。
At home and abroad there is no report at present utilizes ferment dreg, dry huge bacterium glass to make black termitomyces culture medium for major ingredient。
Summary of the invention
The purpose of the present invention is contemplated to overcome the deficiency of existing black termitomyces cultivation technique, it is provided that a kind of cultivate the culture medium of black termitomyces and cultural method with what ferment dreg was major ingredient。Be difficult to fruiting, wood flour used when the nutritional character solved when cultivating black termitomyces in prior art in culture medium does not meet its characteristic requirements, cotton seed hull amount is big, be unfavorable for large-scale production and the problem of sustainable developing environment protection。
For solving the problems referred to above, the present invention adopts following technical proposals:
The culture medium of a kind of black termitomyces of cultivation being major ingredient with ferment dreg, the mass fraction of described culture medium is composed as follows: enzymatic microorganism slag, 35-40 part, dry huge bacterium glass 25-20 part, cotton seed hull 20-25 part, Testa Tritici 18-13 part, sugar 1 part, 1 part of Gypsum Fibrosum, potassium dihydrogen phosphate 0.3 part。
The preferred version of the present invention is:
Described moisture content in medium is 60%-65%。
The pH value of described culture medium is in neutrality。
Described culture medium is to adjust pH value in neutrality with salt of weak acid, it is preferable that salt of weak acid is potassium dihydrogen phosphate。
Another object of the present invention is to provide a kind of method that culture medium of edible fungus being major ingredient with ferment dreg cultivates black termitomyces, comprises the steps:
A. select materials: selecting the dregs after the waste-material-preparing enzymatic microorganism such as kitchen vegetable, fruit, mushroom is major ingredient, after ripe huge bacterium glass harvesting, naturally dry, it is ground into 3-8 millimeter coarse powder as dispensing, adding ageing 2-4 month hardwood sawdust, fresh middle large stretch of Testa Tritici and half an hour are the Gypsum Fibrosum powder of curdled appearance；
B. pretreatment of raw material: dry huge bacterium glass, wood flour water spray are prewetted, mixes with ferment dreg, adds Gypsum Fibrosum, Testa Tritici mixing, and sugar and potassium dihydrogen phosphate stir evenly standby in discharging water；
C. modulation: pretreated Gypsum Fibrosum, Testa Tritici are put in ferment dreg, huge bacterium glass, wood flour, stirs, sweet water adding, to appropriate, is fully mixed thoroughly, standby；
D. pack: raw material loads Polypropylene Bag or the high density low-pressure polyethylene plastic bag of 17-18x35-45 with special sack filling machine, cover special ring cowling seals。
E. sterilizing: by packed for bacterium enter normal pressure or autoclaving, stewing pot more than 2 hours proclamations after truce in steriliser, while hot bacterium bag is moved into cool down room standby；
F. inoculation: when bacterium bag temperature is down to below 30 DEG C, traditional vaccination；
G. bacterium is sent out: 22-26 DEG C of constant temperature, half-light send out bacterium；
H. fruiting: take off plastic bag when seeing bag endepidermis physiological maturity under thermophilic, horizontal (erecting) puts into the furrow of well in advance, earthing 4-5 centimetre, banket after watering flood and strike off furrow face, keep humidity, adjust higher carbon dioxide environment in canopy when earth's surface soil temperature reaches 24-26 DEG C and urge mushroom, seven, medium well time pluck。
Compared with prior art, the unexpected technique effect brought is as follows for technical scheme:
(1) present invention optimizes the proportioning of culture medium, cost is low, it is possible to improve crude protein content in black termitomyces；And the aminoacid of black termitomyces, fat, various vitamin and calcium, phosphorus content are high, thus mouthfeel is good, and commodity value is high, it is simple to marketing。
(2) the beneficial microbe colony that can produce multiple catabolic enzyme that enzymatic microorganism is made up of antibacterial, actinomycetes and enzymatic microorganism, except the nutrient required containing fungus growth promoter, possibly together with vitamin, nucleic acid, growth promotes the unknown factor (UGF) and a large amount of activity beneficial microbe, the utilization of various nutritional labelings in mycelium degraded culture medium can be promoted, thus decomposing materials and soil organic substance, molten phosphorus, potassium decomposing, fixed nitrogen, it is suppressed that harmful microorganism。Plant black termitomyces and have promotion mycelial growth and fruiting effect of buddingging, it is possible to promote the use of in cultivating black termitomyces。
(3) present invention develops the black termitomyces dry huge bacterium glass with the ferment dreg being readily available and part replacement cotton seed hull for culture medium raw material, raw material to solve black termitomyces scale development from now on needs, also can thoroughly solve cost height, development for black termitomyces provides large consumption energy, is conducive to the great development of black termitomyces industry。
(4) culture medium provided by the invention and to utilize culture medium to send out the technique of the black termitomyces of bacterium simple, easy to operate, and the black termitomyces quality better planted out, yield is high, it is beneficial to production in enormous quantities, and reduce cotton seed hull consumption, and not only reducing cost, the Harmless also helping black termitomyces culture medium produces。
Detailed description of the invention
Those skilled in the art can use for reference present disclosure, is suitably modified technological parameter and realizes。Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are considered as including in the present invention。
In order to be further appreciated by the present invention, below in conjunction with embodiment, the present invention is described in detail。
Embodiment 1:
The culture medium of a kind of black termitomyces of cultivation being major ingredient with ferment dreg, the mass fraction of this culture medium is composed as follows: ferment dreg 35 parts, dry huge bacterium glass 25 parts, cotton seed hull 20 parts, 18 parts of Testa Tritici, sugar 1 part, 1 part of Gypsum Fibrosum, potassium dihydrogen phosphate 0.3 part。
Ferment dreg is the residue dreg part after making ferment, and pulling out and draining moisture is 40-60%；
In this ferment dreg except the nutrient required containing fungus growth promoter, possibly together with vitamin, nucleic acid, growth promotes the unknown factor (UGF) and a large amount of activity beneficial microbe, can promote the utilization of various nutritional labelings in mycelium degraded culture medium。
With the method that this culture medium of edible fungus cultivates black termitomyces, step is as follows:
A. select materials: the ferment dreg after selecting the enzymatic microorganism sending out ferment to leach enzyme liquid, moisture is 40-50%, stirs and be mixed after being mixed into dry huge bacterium glass, cotton seed hull, Testa Tritici, Gypsum Fibrosum, brown sugar, calcium superphosphate；
B. pack: adopt polyethylene or the polypropylene plastics pocket of 17X35, pack with machinery, with special capping sealing；
C. sterilizing: by packed for bacterium enter handcart, push steriliser normal pressure or autoclaving, after truce, stewing pot is removed from the pot for 2 hours, bacterium bag moves into cooling room while hot standby；
D. inoculation: bacterium bag temperature is down to the double quick inoculation of inoculation device when 30 DEG C；
E. send out bacterium: constant temperature 22-26 DEG C, half-light send out bacterium, humidity is maintained at 55-70%；
F. earthing: bacterium bag mycelia is cooked furrow, bacterium bag taken off horizontal stroke (erecting) and swings in furrow after sending out bacterium full and proceeding to latter stage of ripening, furrow face is struck off after pouring into flood, pours into little water by upper earthing 4-5 centimetre。
G. urge mushroom: when temperature of shed reaches 25-30 DEG C, and surface temperature reaches more than 25 DEG C, namely enter and urge mushroom to manage。
Applying the black termitomyces of what this culture medium planted out, mycelia shape size is uniform, and water content is high, and mouthfeel is good。The black termitomyces of drying, crude protein content is 32.82%, total sugar 26.79%, crude fat 4.40%, crude fibre 6.38%。
Embodiment 2:
A kind of culture medium of the black termitomyces of cultivation being major ingredient with ferment dreg, the mass fraction of this culture medium is composed as follows: ferment dreg 40 parts, dry huge bacterium glass 20 parts, cotton seed hull 25 parts, 13 parts of Testa Tritici, sugar 1 part, 1 part of Gypsum Fibrosum, potassium dihydrogen phosphate 0.3 part,
Ferment dreg is the residue dreg part after making ferment, and pulling out and draining moisture is 40-60%；
In this ferment dreg except the nutrient required containing fungus growth promoter, possibly together with vitamin, nucleic acid, growth promotes the unknown factor (UGF) and a large amount of activity beneficial microbe, can promote the utilization of various nutritional labelings in mycelium degraded culture medium。
With the method that this culture medium of edible fungus cultivates black termitomyces, step is as follows:
A. select materials: the ferment dreg after selecting the enzymatic microorganism sending out ferment to leach enzyme liquid, moisture is 40-50%, stirs and be mixed after being mixed into dry huge bacterium glass, cotton seed hull, Testa Tritici, Gypsum Fibrosum, brown sugar, calcium superphosphate；
B. pack: adopt polyethylene or the polypropylene plastics pocket of 17X35, pack with machinery, with special capping sealing；
C. sterilizing: by packed for bacterium enter handcart, push steriliser normal pressure or autoclaving, after truce, stewing pot is removed from the pot for 2 hours, bacterium bag moves into cooling room while hot standby；
D. inoculation: bacterium bag temperature is down to the double quick inoculation of inoculation device when 30 DEG C；
E. send out bacterium: constant temperature 22-26 DEG C, half-light send out bacterium, humidity is maintained at 55-70%；
F. earthing: bacterium bag mycelia is cooked furrow, bacterium bag taken off horizontal stroke (erecting) and swings in furrow after sending out bacterium full and proceeding to latter stage of ripening, furrow face is struck off after pouring into flood, pours into little water by upper earthing 4-5 centimetre。
G. urge mushroom: when temperature of shed reaches 25-30 DEG C, and surface temperature reaches more than 25 DEG C, namely enter and urge mushroom to manage。
Applying the black termitomyces of what this culture medium planted out, mycelia shape size is uniform, and water content is high, and mouthfeel is good。The black termitomyces of drying, crude protein content is 32.82%, total sugar 26.79%, crude fat 4.40%, crude fibre 6.38%。
The above is only the preferred embodiment of the present invention, it is noted that for those skilled in the art, under the premise of not departing from the present invention, it is also possible to make some improvement, and these improvement also should be regarded as protection scope of the present invention。
Claims (5)
1. the culture medium cultivating black termitomyces that a kind is major ingredient with ferment dreg, it is characterized in that, the mass fraction of described culture medium is composed as follows: enzymatic microorganism slag, 35-40 part, dry huge bacterium glass 25-20 part, cotton seed hull 20-25 part, Testa Tritici 18-13 part, sugar 1 part, 1 part of Gypsum Fibrosum, potassium dihydrogen phosphate 0.3 part。
2. the culture medium of the black termitomyces of cultivation being major ingredient with ferment dreg according to claim 1, it is characterised in that described moisture content in medium is 60%-65%。
3. the culture medium of the black termitomyces of cultivation being major ingredient with ferment dreg according to claim 1, it is characterised in that the pH value of described culture medium is in neutrality。
4. the culture medium of the black termitomyces of cultivation being major ingredient with ferment dreg according to claim 3, it is characterised in that described culture medium is to adjust pH value in neutrality with salt of weak acid, it is preferable that salt of weak acid is potassium dihydrogen phosphate。
5. the method for the culture medium of edible fungus black termitomyces of cultivation being major ingredient with ferment dreg described in any one of Claims 1-4, comprises the steps:
A. select materials: selecting the dregs after the waste-material-preparing enzymatic microorganism such as kitchen vegetable, fruit, mushroom is major ingredient, after ripe huge bacterium glass harvesting, naturally dry, it is ground into 3-8 millimeter coarse powder as dispensing, adding ageing 2-4 month hardwood sawdust, fresh middle large stretch of Testa Tritici and half an hour are the Gypsum Fibrosum powder of curdled appearance；
B. pretreatment of raw material: dry huge bacterium glass, wood flour water spray are prewetted, mixes with ferment dreg, adds Gypsum Fibrosum, Testa Tritici mixing, and sugar and potassium dihydrogen phosphate stir evenly standby in discharging water；
C. modulation: pretreated Gypsum Fibrosum, Testa Tritici are put in ferment dreg, huge bacterium glass, wood flour, stirs, sweet water adding, to appropriate, is fully mixed thoroughly, standby；
D. pack: raw material loads Polypropylene Bag or the high density low-pressure polyethylene plastic bag of 17-18x35-45 with special sack filling machine, cover special ring cowling seals；
E. sterilizing: by packed for bacterium enter normal pressure or autoclaving, stewing pot more than 2 hours proclamations after truce in steriliser, while hot bacterium bag is moved into cool down room standby；
F. inoculation: when bacterium bag temperature is down to below 30 DEG C, traditional vaccination；
G. bacterium is sent out: 22-26 DEG C of constant temperature, half-light send out bacterium；
H. fruiting: take off plastic bag when seeing bag endepidermis physiological maturity under thermophilic, horizontal (erecting) puts into the furrow of well in advance, earthing 4-5 centimetre, banket after watering flood and strike off furrow face, keep humidity, adjust higher carbon dioxide environment in canopy when earth's surface soil temperature reaches 24-26 DEG C and urge mushroom, seven, medium well time pluck。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610046901.3A CN105684727B (en) | 2016-01-25 | 2016-01-25 | The culture medium and cultural method for the black collybia albuminosa of cultivation expected based on ferment bacteria residue |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610046901.3A CN105684727B (en) | 2016-01-25 | 2016-01-25 | The culture medium and cultural method for the black collybia albuminosa of cultivation expected based on ferment bacteria residue |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106220318A (en) * | 2016-07-20 | 2016-12-14 | 金珠满江农业有限公司 | A kind of biogas residue makes black termitomyces culture medium and application thereof and preparation method |
| CN107258327A (en) * | 2017-07-31 | 2017-10-20 | 安龙县农望种植农民专业合作社 | A kind of cultural method of collybia albuminosa |
| CN107400018A (en) * | 2017-09-18 | 2017-11-28 | 田林县群英农业有限公司 | A kind of cultivation fertilizer of collybia albuminosa and preparation method thereof |
| CN107484554A (en) * | 2017-09-29 | 2017-12-19 | 贵州棒棒食用菌产业有限公司 | A kind of artificial collybia albuminosa cultural method |
| CN107646515A (en) * | 2017-08-30 | 2018-02-02 | 山东省科创食用菌产业技术研究院 | A kind of Termitomyces albuminosus with black skin cultural method |
| CN108401782A (en) * | 2018-02-02 | 2018-08-17 | 金珠满江农业有限公司 | Using huge monarch's grass as black collybia albuminosa culture medium of major ingredient and the preparation method and application thereof |
| CN109380063A (en) * | 2018-09-27 | 2019-02-26 | 辽宁三友农业生物科技有限公司 | Utilize the method for cordyceps culturing medium waste production edible fungi liquid strain |
| CN110896924A (en) * | 2018-09-17 | 2020-03-24 | 杨易 | Artificial cultivation and planting technology for russula |
| CN111837809A (en) * | 2020-07-27 | 2020-10-30 | 芜湖野树林生物科技有限公司 | Black termitomyces albuminosus culture medium and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444170A (en) * | 2008-12-16 | 2009-06-03 | 许忠 | Strain separation method of apricot ormer mushroom and cultivating method thereof |
| CN103621310A (en) * | 2012-08-25 | 2014-03-12 | 琚江河 | Wild beef mushroom successfully trained and cultivated by using mountain grass and mushroom residues |
| CN104163675A (en) * | 2014-07-30 | 2014-11-26 | 晶叶（青岛）生物科技有限公司 | Culture medium suitable for cordyceps militaris liquid fermentation |
| CN104744126A (en) * | 2015-03-01 | 2015-07-01 | 邬方成 | Preparation method of pleurotus cornucopiae cultivation material |
| CN105052551A (en) * | 2015-08-14 | 2015-11-18 | 梁德政 | Method for fast and high-yield cultivation of oyster mushroom at high-temperature season |
-
2016
- 2016-01-25 CN CN201610046901.3A patent/CN105684727B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444170A (en) * | 2008-12-16 | 2009-06-03 | 许忠 | Strain separation method of apricot ormer mushroom and cultivating method thereof |
| CN103621310A (en) * | 2012-08-25 | 2014-03-12 | 琚江河 | Wild beef mushroom successfully trained and cultivated by using mountain grass and mushroom residues |
| CN104163675A (en) * | 2014-07-30 | 2014-11-26 | 晶叶（青岛）生物科技有限公司 | Culture medium suitable for cordyceps militaris liquid fermentation |
| CN104744126A (en) * | 2015-03-01 | 2015-07-01 | 邬方成 | Preparation method of pleurotus cornucopiae cultivation material |
| CN105052551A (en) * | 2015-08-14 | 2015-11-18 | 梁德政 | Method for fast and high-yield cultivation of oyster mushroom at high-temperature season |
Non-Patent Citations (1)
| Title |
|---|
| 贡国勇: "《西北菌草栽培双孢蘑菇理论与实践》", 30 June 2011 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106220318A (en) * | 2016-07-20 | 2016-12-14 | 金珠满江农业有限公司 | A kind of biogas residue makes black termitomyces culture medium and application thereof and preparation method |
| CN107258327A (en) * | 2017-07-31 | 2017-10-20 | 安龙县农望种植农民专业合作社 | A kind of cultural method of collybia albuminosa |
| CN107646515A (en) * | 2017-08-30 | 2018-02-02 | 山东省科创食用菌产业技术研究院 | A kind of Termitomyces albuminosus with black skin cultural method |
| CN107400018A (en) * | 2017-09-18 | 2017-11-28 | 田林县群英农业有限公司 | A kind of cultivation fertilizer of collybia albuminosa and preparation method thereof |
| CN107484554A (en) * | 2017-09-29 | 2017-12-19 | 贵州棒棒食用菌产业有限公司 | A kind of artificial collybia albuminosa cultural method |
| CN108401782A (en) * | 2018-02-02 | 2018-08-17 | 金珠满江农业有限公司 | Using huge monarch's grass as black collybia albuminosa culture medium of major ingredient and the preparation method and application thereof |
| CN110896924A (en) * | 2018-09-17 | 2020-03-24 | 杨易 | Artificial cultivation and planting technology for russula |
| CN109380063A (en) * | 2018-09-27 | 2019-02-26 | 辽宁三友农业生物科技有限公司 | Utilize the method for cordyceps culturing medium waste production edible fungi liquid strain |
| CN109380063B (en) * | 2018-09-27 | 2020-10-27 | 辽宁三友农业生物科技有限公司 | Method for producing edible fungus liquid strain by using cordyceps militaris culture medium waste |
| CN111837809A (en) * | 2020-07-27 | 2020-10-30 | 芜湖野树林生物科技有限公司 | Black termitomyces albuminosus culture medium and preparation method and application thereof |
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