CN101444170A - Strain separation method of apricot ormer mushroom and cultivating method thereof - Google Patents

Strain separation method of apricot ormer mushroom and cultivating method thereof Download PDF

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CN101444170A
CN101444170A CNA200810243834XA CN200810243834A CN101444170A CN 101444170 A CN101444170 A CN 101444170A CN A200810243834X A CNA200810243834X A CN A200810243834XA CN 200810243834 A CN200810243834 A CN 200810243834A CN 101444170 A CN101444170 A CN 101444170A
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cultivating
composts
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mushroom
water
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CN101444170B (en
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许忠
许腾龙
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许忠
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Abstract

The invention discloses a strain separation method of apricot ormer mushroom and a cultivating method thereof. The cultivating method comprises the following step: cultivating strains which derive from apricot ormer mushroom-2 strains sold by Gaoyou Edible Fungi Institute of Science and has high yield and strong stress resistance; the cultivating process of the strains is as follows: preparing composts, bagging and sterilizing; inoculating; managing myceliums; opening the bag and leading the mushroom out; managing the mushroom; and harvesting properly. The invention not only achieves the strain scale plantation of the apricot ormer mushroom by selecting different culture media and different culture conditions, but also shortens the strain planting period of the apricot ormer mushroom, so that the apricot ormer mushroom to be planted has strong resistance, high biological efficiency, good quality and the like. By adopting the technical scheme, the biologic conversion rate of the apricot ormer mushroom achieves above 145%; the planting technology is easy to master; and the source of the culture media is wide. The invention is particularly suitable for the scale plantation in wide rural areas, thereby improving the planting efficiency.

Description

A kind of strain separation method of Xingbao mushroom and cultivation method thereof
Technical field
The present invention relates to the breeding method of a kind of mushroom, relate in particular to a kind of strain separation method and cultivation method thereof of Xingbao mushroom.
Background technology
Edible mushroom is a kind of high protein, low fat, be rich in amino acid, vitamin and mineral matter and various polysaccharide and the low gourmet food of heat, to improve body immunity, reducing blood lipid, protect the liver, anti-cancer and cancer-preventing, anti-ageing waiting for a long time have tangible dietary function.Often edible edible mushroom can prophylaxis of hypertension and treatment liver and enterogastric diseases, and edible mushroom is described as " mountain delicacy ", is the third-largest food sources of the mankind after plant food, animal food.Xingbao mushroom, formal name used at school: Pleurotus eryngii (DC.ex.Fr.) Quel, call perverse celery and pick up the ears.Be under the jurisdiction of Eumycota, Basidiomycetes, Agaricales, Pleurotaceae, Pleurotus.Xingbao mushroom bacterial context plumpness, quality is tender and crisp, stem dense structure, solid, milky white particularly, can all eat, and stem is than the more crisp cunning of cap, tasty and refreshing, being called as " flat mushroom king ", " dried scallop mushroom ", is to develop the rare edible mushroom new varieties edible, medicinal, dietotherapy that integrate of cultivating successfully in recent years.The mushroom body has almond flavor, the meat plumpness, mouthfeel is fresh and tender, taste delicate fragrance, mineral matters such as rich in proteins, carbohydrate, vitamin and calcium, magnesium, copper, zinc, can improve immune function of human body, human body is had anticancer, reducing blood lipid, norcholesterol, promotion gastro-intestinal digestion, ease constipation stomach, prevents the effect of cardiovascular disease; Oligosaccharides is abundant in the fruit body of Xingbao mushroom, and oligosaccharides is the sugar of 9-13 carbon molecules, and this sugar content in other food is less, but is necessary for human body, and the people can make enteron aisle unobstructed after absorbing, and makes the skin delicacy, Gu Xingbao mushroom has the effect of whole intestines and beauty treatment; Xingbao mushroom has almond flavor and as the mouthfeel of abalone, be fit to fresh-keeping, processing, the difference of the flat mushroom of Xingbao mushroom and other general kinds, mushroom, coprinus comatus etc. is: the time of preserving after tissue tight, high resilience, the harvesting more generally pesters long, and the utmost point is liked by people.But Xingbao mushroom will be cultivated with grog, and technology essential factor is had relatively high expectations, complex manufacturing, thereby restricted the Xingbao mushroom large-scale planting, cultivation benefit is very low.
Summary of the invention
Technical problem to be solved by this invention provides a kind of strain separation method of Xingbao mushroom and the cultivation method of bacterial strain thereof, its objective is to promote local large-scale planting, improves cultivation benefit.
To achieve these goals, the technical scheme that the present invention takes is: a kind of strain separation method of Xingbao mushroom, step by the following method:
One, female separation and Culture of planting:
(1) the component prescription of composts or fertilisers of cultivating: peeled potatoes 200g, agar powder 20g, glucose 20g, water 1000ml, pH value are 7~7.5;
Or peeled potatoes 180g, wheat bran 20g, agar powder 20g, glucose 20g, water 1000ml; Or in above-mentioned prescription, add potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g again;
(2) making of composts or fertilisers of cultivating: cut out eye after peeled potatoes is cleaned and thinly slice, put into water or add wheat bran and be heated to 100 ℃, insulation 30~35min, filter, getting filtrate adding agar powder makes it melt back adding glucose, potassium dihydrogen phosphate and magnesium sulfate fully, and supplying water consumption in the original formulation, 100 ℃ of insulation 25~30min filter;
Be filled in vitro filtering good solution branch, charge weight is 1/4 of a test tube, and the test tube mouth has been filled in tampon, wraps an end that is plugged with tampon with brown paper, puts into high-pressure sterilizing pot, rises to 1.5 atmospheric pressure sterilizations 3 hours fast; Or normal pressure was sterilized 10 hours for following 100 ℃;
(3) inoculated and cultured: gather new fresh sporophore, and carry out epidermis sterilization, get a fritter fruit body and put into and to be cooled to 28 ℃ aseptic condition under in vitro, and postvaccinal test tube is put the inclined-plane; In 18~22 ℃ environment, can cover with mycelia in 15~20 days, be female the kind;
Two, the production of original seed
1) prescription of composts or fertilisers of cultivating is: cotton seed hulls or wheat straw or straw or corncob 75%, wheat bran or rice bran 18%, corn flour 5%, the plaster of paris 1%, sucrose 0.5%, quicklime 0.5%, add potassium dihydrogen phosphate 0.3% by above-mentioned batching total amount again, magnesium sulfate 0.2%, each component is all percentage by weight, and pH value is 6.5~7;
(2) composts or fertilisers of cultivating is made: first water was fully moistening before cotton seed hulls or wheat straw or straw or corncob crop stalk used, heap fermentation 3~5 days, quicklime, the plaster of paris, potassium dihydrogen phosphate, magnesium sulfate is soluble in water, crop stalk after adding rice bran, corn flour, wheat bran, sucrose and the fermentation stirs, the moisture content of composts or fertilisers of cultivating is 65%, the composts or fertilisers of cultivating that stirs in time branch is filled in the culture bottle, and has filled in tampon, wraps an end that is plugged with tampon with brown paper;
(3) sterilization: culture bottle is in time put into the high-pressure sterilizing pot sterilization, and 1.5 atmospheric pressure were sterilized 3 hours; Or normal-pressure sterilization, sterilized 10 hours for 100 ℃;
(4) inoculation: the temperature of culture bottle is inoculated in desinfection chamber or inoculating hood for 26~28 ℃, 5 bottles of original seeds of every test tube switching;
(5) cultivate: with postvaccinal original seed bottle be placed on totally, the indoor cultivation of drying, ventilation, shading, temperature is controlled at 23~25 ℃, mycelia was covered with the original seed bottle in 32~36 days, reach that mycelia is dense, caking capacity by force, no living contaminants;
Three, the production of cultivated species:
(1) prescription of composts or fertilisers of cultivating is: cotton seed hulls or wheat straw or straw or corncob 77%, and wheat bran or rice bran 15%, corn flour 5%, the plaster of paris 1%, sucrose 1%, quicklime 1%, each component is all percentage by weight, and pH value is 6.5~7;
(2) composts or fertilisers of cultivating is made: first water was fully moistening before cotton seed hulls or wheat straw or straw or corncob crop stalk used, heap fermentation 3~5 days, quicklime, the plaster of paris, potassium dihydrogen phosphate, magnesium sulfate is soluble in water, crop stalk after adding rice bran, corn flour, wheat bran, sucrose and the fermentation stirs, the moisture content of composts or fertilisers of cultivating is 65%, the composts or fertilisers of cultivating that stirs in time branch is filled in the culture bottle, and has filled in tampon, wraps an end that is plugged with tampon with brown paper;
(3) sterilization: culture bottle is in time put into the high-pressure sterilizing pot sterilization, and 1.5 atmospheric pressure were sterilized 3 hours; Or normal-pressure sterilization, sterilized 10 hours for 100 ℃;
(4) inoculation: the temperature of culture bottle is inoculated in desinfection chamber or inoculating hood for 26~28 ℃, 50 bottles of cultivated speciess of every original seed bottle switching;
(5) cultivate: with postvaccinal original seed bottle be placed on totally, the indoor cultivation of drying, ventilation, shading, temperature is controlled at 20~25 ℃, mycelia was covered with the cultivated species bottle in 32~36 days, reach that mycelia is dense, caking capacity by force, no living contaminants.
Described female kind composts or fertilisers of cultivating is peeled potatoes 200g, agar powder 20g, glucose 20g, water 1000ml.
Described female kind composts or fertilisers of cultivating is peeled potatoes 180g, wheat bran 20g, agar powder 20g, glucose 20g, water 1000ml.
Described female kind composts or fertilisers of cultivating is peeled potatoes 180g, wheat bran 20g, agar powder 20g, glucose 20g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, water 1000ml.
Described female kind composts or fertilisers of cultivating is peeled potatoes 200g, agar powder 20g, glucose 20g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, water 1000ml.
A kind of cultivation method of bacterial strain of Xingbao mushroom is characterized in that:
A) prescription of composts or fertilisers of cultivating is: cotton seed hulls or wheat straw or straw or corncob 75~80%, wheat bran or rice bran 5~12%, the argol of poultry or livestock just 8~10%, corn flour 3~8%, the plaster of paris 1~2%, sucrose 0.5~1.5%, quicklime 0.5~1.5% adds potassium dihydrogen phosphate 0.1~0.3%, magnesium sulfate 0.1~0.2% by above-mentioned batching total amount again, each component is all percentage by weight, and pH value is 6.5~7;
B) composts or fertilisers of cultivating is made: first water was fully moistening before cotton seed hulls or wheat straw or straw or corncob crop stalk used, heap fermentation 5~7 days; Heap fermentation behind the collecting dung of poultry or livestock every turning in 3~4 days once, ferments to shakeout after 28~32 days and dries, and pulverizes standby;
Preparation method is: quicklime, the plaster of paris, potassium dihydrogen phosphate, magnesium sulfate are soluble in water, cotton seed hulls that adding ferments or wheat straw or straw or corncob are prewetted, the argol that adds rice bran, corn flour, wheat bran, sucrose and standby poultry or livestock more just uses agitator to stir 2 times, the composts or fertilisers of cultivating moisture content is controlled at 65%, the composts or fertilisers of cultivating that stirs in time branch is filled in the cultivation bag, long 17~the 20cm of cultivation bag, wide 30~33cm, cultivate material is compacting successively, and work loading height is that 22~24cm seals with cord or supporting seal mouth ring;
C) sterilization: cultivation bag is in time put into the high-pressure sterilizing pot sterilization, and 1.5 atmospheric pressure were sterilized 3 hours; Or normal-pressure sterilization, sterilized 10 hours for 100 ℃;
D) inoculation: the temperature of culture bottle is inoculated in desinfection chamber or inoculating hood for 26~28 ℃, and bacterial classification must cover full composts or fertilisers of cultivating surface, and every bottle of cultivated species can be inoculated 20~30 bags;
E) mycelium management:
Postvaccinal cultivation bag moves into clean indoor, and the control temperature is sent out bacterium for 22~25 ℃, also can move into and send out bacterium in the green house of vegetables after sterilization, and control air humidity 60~63% is ventilated 2~3 every day, and each 1~2 hour, strict shading, control CO 2Concentration be 500~2000ppm, mycelium can cover with the bacterium bag in 32~35 days;
F) row's bag fruiting:
The green house of vegetables that 6m is wide is divided into 4 furrow, the wide 1.1m of every furrow, dark 0.05m, the cultivation bag of sending out bacterium good is arranged in furrow, anyhow embark on journey, near discharging, open sack between bag and the bag, with the sack warp, sack is higher than about charge level 5cm, and the control greenhouse temperature is 6~22 ℃, unified the cultivation bag of sending out well bacterium is carried out the alternating temperature stimulation, 5~7 days repeatedly, flower bud formed mushroom in a large number;
G) management of producing mushroom:
The control air humidity is sprayed clear water every day 1~2 time, keeps relative air humidity 80~90% in the mushroom canopy; Ventilate each 2 hours every day 3 times; Keep 6~22 ℃ of temperature of shed, can not surpass 22 ℃, and keep in the canopy scattered light being arranged;
H) gather:
Cap parachute-opening degree 40%, general stem grows to 5~10cm, bacteria cover diameter 2~5cm, the fruit body size is well-balanced, can gather.
The Xingbao mushroom required nutrient component of each stage of growing can be divided into carbon source, nitrogenous source, mineral salt and growth hormone four big classes, Xingbao mushroom is a kind of wood decay fungi, there is not chlorophyll in its body, can not carry out synthetic its required nutrition of photosynthesis, nutraceutical nutrient is main in decomposition, the absorption composts or fertilisers of cultivating but rely on.
The antitumorigenic substance that contains in the edible mushroom is a polysaccharide, also comprise effective medicinal components such as lecithin, nucleic acid and lipids, use little culture vessels such as cultivation bag disclosed by the invention, adopt sterilization treatment, sucrose and starch based nutrients are added wherein, can effectively improve property of medicine composition synthetic of edible mushroom; If use conventional method of directly cultivating in soil, then can be because of the degraded of other microorganism, and can not get expected effect, reduce its effective component.
A large amount of crop stalks is used in the medium of edible mushroom, effectively reduces the smoke contamination that the crop straw on-site incineration produces, and the medium by product can improve soil structure as organic manure, promotes benign ecological cycles, helps developing a circular economy.
The bacterial strain plantation of Xingbao mushroom realizes in green house of vegetables.Greenhouse temperature is steady relatively, and air humidity is higher relatively, and cultivation condition is relatively easily controlled, and cultivation condition is convenient to regulation and control, and environment disinfected is easy to operate, has guaranteed the primary condition that edible fungi growth is grown.
The prescription of described composts or fertilisers of cultivating is: cotton seed hulls or wheat straw or straw or corncob 78%, wheat bran or rice bran 8%, the argol of poultry or livestock just 9%, corn flour 3%, the plaster of paris 1%, sucrose 0.5%, quicklime 0.5%, add potassium dihydrogen phosphate 0.3% by above-mentioned batching total amount again, magnesium sulfate 0.1%, each component is all percentage by weight.
The ight soil of described composts or fertilisers of cultivating poultry can be the ight soil of chicken, duck or goose; The ight soil of domestic animal can be the ight soil of ox or sheep, without the ight soil of pig.
Composts or fertilisers of cultivating behind the Xinbao mushroom culturing bacterial strain is also field earth backing processing on the spot.Can improve soil quality, improve the fertility of field soil, help protecting the field and protect soil.
Edible mushroom is secretase albumen in growth and development process, and the degraded composts or fertilisers of cultivating discharges the nutriments that plant absorbing is utilized that are easy to more.Cultivated and contained nutrients such as higher organic matter and nitrogen, phosphorus, potassium in the discarded object of Xingbao mushroom, also contain the hormone and the trace element that promote plant growing, discarded object is also field earth backing processing on the spot, also can reduce the fertilizer amount of vegetable growing, improve yield of vegetables and quality, improve soil organic matter content, strengthen the anti-fertile ability of vegetables.
Compared with prior art, adopt the method for cultivation of the present invention, the kind that seed selection is fit to, thermophilic is wide, and it is fast to send out bacterium, and fruiting is neat, the cultivation cycle is short, strong stress resistance, biology efficient height, quality better etc., improved the product quality of Xingbao mushroom comprehensively, changed traditional cultivation structure and pattern, and implementing bag cultivating, to produce Xingbao mushroom mushroom shape neat, has high commodity value; Its output height, cycle are short, and cost is low, plants easyly, promote local large-scale planting, the raising cultivation benefit.Xingbao mushroom bag cultivating technology is for the utilization that improves the booth in winter provides good varieties of plant; The composts or fertilisers of cultivating of planting Xingbao mushroom has simultaneously made full use of crop stalks such as the straw that produces in the agricultural production, wheat straw, wood chip, boll hull, effectively alleviated of the pollution of the flue dust of crop stalk burning generation to atmosphere, using discarded later composts or fertilisers of cultivating is good organic manure, can increase the fertility of soil, improve the air capacity of soils of soil, thereby promote the benign cycle of agricultural production.
Embodiment
Description below by to specific embodiment is described in further detail the specific embodiment of the present invention.
The bacterial classification that the present invention uses derives from Xingbao mushroom-2 bacterial classification that Gaoyou science edible mushroom research institute sells, this bacterial classification output height, strong stress resistance.
The separation and Culture technology.
Embodiment 1
Take by weighing peeled potatoes 200g, cutting out eye after cleaning thinly slices, put into water and be heated to 100 ℃ of insulation 30~35min, filter, get filtrate adding agar powder 20g and make it melt back adding glucose 20g, potassium dihydrogen phosphate 1.5g and magnesium sulfate 1g fully, and supply the water consumption in the original formulation, 100 ℃ of insulation 25~30min, filter, the control pH value is 7~7.5;
Be filled in vitro filtering good solution branch, charge weight is 1/4 of a test tube, and the test tube mouth has been filled in tampon, wraps an end that is plugged with tampon with brown paper, puts into high-pressure sterilizing pot, rises to 1.5 atmospheric pressure sterilizations 3 hours fast; Or normal pressure was sterilized 10 hours for following 100 ℃; Gather new fresh sporophore, and carry out epidermis sterilization, get a fritter fruit body and put into and to be cooled to 28 ℃ aseptic condition under in vitro, and postvaccinal test tube is put the inclined-plane; In 18~22 ℃ environment, can cover with mycelia in 15~20 days, be female the kind.
Embodiment 2
Take by weighing peeled potatoes 200g, cutting out eye after cleaning thinly slices, put into water and be heated to 100 ℃, insulation 30~35min filters, and gets filtrate adding agar powder 20g and makes it melt back adding glucose 20g fully, and supply water consumption in the original formulation, 100 ℃ of insulation 25~30min filter, and the control pH value is 7~7.5.Following method is with embodiment 1.
Embodiment 3
Take by weighing peeled potatoes 180g, cutting out eye after cleaning thinly slices, put into water or add wheat bran and be heated to 100 ℃, insulation 30~35min filters, and gets filtrate adding agar powder 20g and makes it melt back adding glucose 20g, potassium dihydrogen phosphate 1.5g and magnesium sulfate 1g fully, and supply water consumption in the original formulation, 100 ℃ of insulation 25~30min filter, and the control pH value is 7~7.5.Following method is with embodiment 1.
The original seed culture technique
Embodiment 4
Take by weighing cotton seed hulls 7.5kg, first water is fully moistening, heap fermentation 3~5 days.
Quicklime 50g, plaster of paris 100g, potassium dihydrogen phosphate 30g, magnesium sulfate 20g is soluble in water, cotton seed hulls after adding rice bran 1.8kg, corn flour 0.5kg, sucrose 50g and the fermentation stirs, the moisture content of composts or fertilisers of cultivating is 65%, it is in the 500g jar that the timely branch of the composts or fertilisers of cultivating that stirs is filled to discarded capacity, and seal with disregard message paper or polypropylene film, wrap an end that seals with brown paper; The pH value of control composts or fertilisers of cultivating is 65~7.
Culture bottle is in time put into the high-pressure sterilizing pot sterilization, and 1.5 atmospheric pressure were sterilized 3 hours; Or normal-pressure sterilization, sterilized 10 hours for 100 ℃, naturally cooling; Inoculate in 26~28 ℃ of immigration desinfection chambers of the temperature of the culture bottle after the sterilization or the inoculating hood, getting female kind of a fritter under aseptic condition puts in the middle of the bottle charge level, 5 bottles of original seeds of every test tube switching, method when bottling with composts or fertilisers of cultivating is sealed, with postvaccinal original seed bottle be placed on totally, the indoor cultivation of drying, ventilation, shading, temperature is controlled at 23~25 ℃, relative air humidity 70%, mycelia was covered with the original seed bottle in 32~36 days, reached that mycelia is dense, caking capacity is strong, no living contaminants.
Embodiment 5
Take by weighing corncob 7.5kg, it is fully moistening to pulverize water earlier again, heap fermentation 3~5 days.
Quicklime 50g, plaster of paris 100g, potassium dihydrogen phosphate 30g, magnesium sulfate 20g is soluble in water, corncob after adding wheat bran 1.8kg, corn flour 0.5kg, sucrose 50g and the fermentation stirs, the moisture content of composts or fertilisers of cultivating is 65%, the composts or fertilisers of cultivating that stirs in time branch is filled in the discarded infusion bottle, and filled in tampon, wrap an end that is plugged with tampon with brown paper.Following method is with embodiment 4.
The production of cultivated species:
Embodiment 6
Take by weighing wheat straw 7.7kg, first water is fully moistening, heap fermentation 3~5 days.
Quicklime 100g, plaster of paris 100g is soluble in water, and the wheat straw after adding wheat bran 1.5kg, corn flour 500g, sucrose 100g and the fermentation stirs, and the moisture content of composts or fertilisers of cultivating is 65%, and pH value is 6.5~7; The composts or fertilisers of cultivating that stirs in time branch is filled in the culture bottle, and has filled in tampon, wraps an end that is plugged with tampon with brown paper; Culture bottle is in time put into the high-pressure sterilizing pot sterilization, and 1.5 atmospheric pressure were sterilized 3 hours; Or normal-pressure sterilization, sterilized 10 hours for 100 ℃; The temperature of culture bottle is inoculated in desinfection chamber or inoculating hood for 26~28 ℃, 50 bottles of cultivated speciess of every original seed bottle switching; With postvaccinal original seed bottle be placed on totally, the indoor cultivation of drying, ventilation, shading, temperature is controlled at 20~25 ℃, mycelia was covered with the cultivated species bottle in 32~36 days, reached that mycelia is dense, caking capacity strong, no living contaminants.
The cultivation method of bacterial strain
Embodiment 7
Heap fermentation behind the collecting dung of poultry or livestock every turning in 3~4 days once, ferments to shakeout after 28~32 days and dries, and pulverizes standby.
Take by weighing cotton seed hulls 7.5kg, first water is fully moistening, heap fermentation 5~7 days.
Quicklime 50g, plaster of paris 100g, potassium dihydrogen phosphate 10g, magnesium sulfate 10g are soluble in water, adding the cotton seed hulls that ferments prewets, add again rice bran 1kg, corn flour 300g and sucrose 50g and standby poultry argol just 1kg use agitator to stir 2 times, the composts or fertilisers of cultivating moisture content is controlled at 65%, the composts or fertilisers of cultivating that stirs in time branch is filled in the cultivation bag, cultivation bag uses the low-pressure polyethylene plastic sack, bag is of a size of long 17cm, wide 33cm, compacting successively after cultivate material is packed in the bag, work loading height is 22~24cm, seals with cord or supporting seal mouth ring; Cultivation bag is in time put into the high-pressure sterilizing pot sterilization, and 1.5 atmospheric pressure were sterilized 3 hours; Or normal-pressure sterilization, sterilized 10 hours for 100 ℃; The temperature of cultivation bag is inoculated in desinfection chamber or inoculating hood for 26~28 ℃, and bacterial classification must cover full composts or fertilisers of cultivating surface, and every bottle of cultivated species can be inoculated 20~30 bags; Postvaccinal cultivation bag moves into clean indoor, and the control temperature is sent out bacterium for 22~25 ℃, and control air humidity 60~63% is ventilated 2~3 every day, and each 1~2 hour, strict shading, control CO 2Concentration be 500~2000ppm, mycelium can cover with the bacterium bag in 32~35 days; The green house of vegetables that 6m is wide is divided into 4 furrow, the wide 1.1m of every furrow, dark 0.05m, the cultivation bag of sending out bacterium good is arranged in furrow, anyhow embark on journey, near discharging, open sack between bag and the bag, with the sack warp, sack is higher than about charge level 5cm, unified the cultivation bag of sending out well bacterium is carried out the alternating temperature stimulation, have a mind to widen day and night temperature, 5~7 days repeatedly, flower bud formed mushroom in a large number;
The control air humidity is sprayed clear water every day 1~2 time, keeps relative air humidity 80~90% in the mushroom canopy; Ventilate each 2 hours every day 3 times; Keep 6~22 ℃ of temperature of shed, can not surpass 22 ℃, and keep in the canopy scattered light being arranged; Cap parachute-opening degree 40%, general stem grows to 5~10cm, bacteria cover diameter 2~5cm, the fruit body size is well-balanced, can gather.
Embodiment 8
Take by weighing corncob 7.8kg, it is fully moistening earlier corncob to be pulverized the back water before using, heap fermentation 5~7 days., wheat bran 800g, the argol of domestic animal is 900g just, corn flour 300g, plaster of paris 100g, sucrose 50g, quicklime 50g adds potassium dihydrogen phosphate 30g again, magnesium sulfate 10g, the preparation method of composts or fertilisers of cultivating and embodiment 7 are together.Cultivation bag moves in the green house of vegetables after sterilization and sends out bacterium, the temperature control of booth and embodiment 7 with, cultivation and management method and embodiment 7 are together.
Embodiment 9
First water is fully moistening before taking by weighing wheat straw 8.0kg use, heap fermentation 5~7 days; Rice bran 500g, the argol of poultry or livestock is 800g just, corn flour 300g, plaster of paris 200g, sucrose 150g, quicklime 50g adds potassium dihydrogen phosphate 10g again, magnesium sulfate 15g.Cultivation and management method and embodiment 7 are together.
Xingbao mushroom nutrient component analysis tabulation
Nutrient component Content Calculate the basis
Crude protein 25 The dried mushroom % of 100 grams
Fat 1.4 The dried mushroom % of 100 grams
Raw fiber 6.9 The dried mushroom % of 100 grams
Total amino acid content 16.644 The dried mushroom % of 100 grams
Vitamin E 7.6 milligram The dried mushroom of 100 grams
Each bacterial strain production cycle is analyzed
Output (g/ bag) Growth time (my god) Biological transformation ratio (%)
175.8 121 145.3
181 125 144.8
178.5 122 146.3
164.5 113 145.6
181.4 125 145.1
168.7 116 145.4
192.3 131 146.8
165.8 115 144.2
186.8 128 145.9
182.8 126 145.1
165.6 114 145.3
173.6 119 145.9
Analysis result shows: average yield is the 176.4g/ bag, and biological transformation ratio reaches 145.48%.The prescription of batch is got any number combination in given number range, to the not influence of output of strain of Pleurotus eryngii, and the strict on request control of planting environment, the growth of Xingbao mushroom has uniformity, but large-scale planting.
Adopt composts or fertilisers of cultivating Xinbao mushroom culturing bacterial strain of the present invention, its biological transformation ratio reaches 140~150%, doubles than the biological transformation ratio about 70% of original planting technology method; Its growth has uniformity, is fit to large-scale planting.
The present invention can use without prejudice to the concrete form of spirit of the present invention or principal character and summarize.Above-mentioned embodiment only is can not limit the present invention to explanation of the present invention, and therefore, implication suitable with claims of the present invention and any change in the scope all should be thought to be included in the scope of claims.

Claims (9)

1, a kind of strain separation method of Xingbao mushroom is characterized in that: step by the following method:
One, female separation and Culture of planting:
(1) the component prescription of composts or fertilisers of cultivating: peeled potatoes 200g, agar powder 20g, glucose 20g, water 1000ml, pH value are 7~7.5;
Or peeled potatoes 180g, wheat bran 20g, agar powder 20g, glucose 20g, water 1000ml; Or in above-mentioned prescription, add potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g again;
(2) making of composts or fertilisers of cultivating: cut out eye after peeled potatoes is cleaned and thinly slice, put into water or add wheat bran and be heated to 100 ℃, insulation 30~35min, filter, getting filtrate adding agar powder makes it melt back adding glucose and potassium dihydrogen phosphate or magnesium sulfate fully, and supplying water consumption in the original formulation, 100 ℃ of insulation 25~30min filter;
Be filled in vitro filtering good solution branch, charge weight is 1/4 of a test tube, and the test tube mouth has been filled in tampon, wraps an end that is plugged with tampon with brown paper, puts into high-pressure sterilizing pot, rises to 1.5 atmospheric pressure sterilizations 3 hours fast; Or normal pressure was sterilized 10 hours for following 100 ℃;
(3) inoculated and cultured: gather new fresh sporophore, and carry out epidermis sterilization, get a fritter fruit body and put into and to be cooled to 28 ℃ aseptic condition under in vitro, and postvaccinal test tube is put the inclined-plane; In 18~22 ℃ environment, can cover with mycelia in 15~20 days, be female the kind;
Two, the production of original seed
1) prescription of composts or fertilisers of cultivating is: cotton seed hulls or wheat straw or straw or corncob 75%, wheat bran or rice bran 18%, corn flour 5%, the plaster of paris 1%, sucrose 0.5%, quicklime 0.5%, add potassium dihydrogen phosphate 0.3% by above-mentioned batching total amount again, magnesium sulfate 0.2%, each component is all percentage by weight, and pH value is 6.5~7;
(2) composts or fertilisers of cultivating is made: first water was fully moistening before cotton seed hulls or wheat straw or straw or corncob crop stalk used, heap fermentation 3~5 days, quicklime, the plaster of paris, potassium dihydrogen phosphate, magnesium sulfate is soluble in water, crop stalk after adding rice bran or wheat bran, corn flour, sucrose and the fermentation stirs, the moisture content of composts or fertilisers of cultivating is 65%, the composts or fertilisers of cultivating that stirs in time branch is filled in the culture bottle, and has filled in tampon, wraps an end that is plugged with tampon with brown paper;
(3) sterilization: culture bottle is in time put into the high-pressure sterilizing pot sterilization, and 1.5 atmospheric pressure were sterilized 3 hours; Or normal-pressure sterilization, sterilized 10 hours for 100 ℃;
(4) inoculation: the temperature of culture bottle is inoculated in desinfection chamber or inoculating hood for 26~28 ℃, 5 bottles of original seeds of every test tube switching;
(5) cultivate: with postvaccinal original seed bottle be placed on totally, the indoor cultivation of drying, ventilation, shading, temperature is controlled at 23~25 ℃, mycelia was covered with the original seed bottle in 32~36 days, reach that mycelia is dense, caking capacity by force, no living contaminants;
Three, the production of cultivated species:
(1) prescription of composts or fertilisers of cultivating is: cotton seed hulls or wheat straw or straw or corncob 77%, and wheat bran or rice bran 15%, corn flour 5%, the plaster of paris 1%, sucrose 1%, quicklime 1%, each component is all percentage by weight, and pH value is 6.5~7;
(2) composts or fertilisers of cultivating is made: first water was fully moistening before cotton seed hulls or wheat straw or straw or corncob crop stalk used, heap fermentation 3~5 days, quicklime, the plaster of paris, potassium dihydrogen phosphate, magnesium sulfate is soluble in water, crop stalk after adding rice bran, corn flour, wheat bran, sucrose and the fermentation stirs, the moisture content of composts or fertilisers of cultivating is 65%, the composts or fertilisers of cultivating that stirs in time branch is filled in the culture bottle, and has filled in tampon, wraps an end that is plugged with tampon with brown paper;
(3) sterilization: culture bottle is in time put into the high-pressure sterilizing pot sterilization, and 1.5 atmospheric pressure were sterilized 3 hours; Or normal-pressure sterilization, sterilized 10 hours for 100 ℃;
(4) inoculation: the temperature of culture bottle is inoculated in desinfection chamber or inoculating hood for 26~28 ℃, 50 bottles of cultivated speciess of every original seed bottle switching;
(5) cultivate: with postvaccinal original seed bottle be placed on totally, the indoor cultivation of drying, ventilation, shading, temperature is controlled at 20~25 ℃, mycelia was covered with the cultivated species bottle in 32~36 days, reach that mycelia is dense, caking capacity by force, no living contaminants.
2, the strain separation method of a kind of Xingbao mushroom according to claim 1 is characterized in that: described female kind composts or fertilisers of cultivating is peeled potatoes 200g, agar powder 20g, glucose 20g, water 1000ml.
3, the strain separation method of a kind of Xingbao mushroom according to claim 1 is characterized in that: described female kind composts or fertilisers of cultivating is peeled potatoes 180g, wheat bran 20g, agar powder 20g, glucose 20g, water 1000ml.
4, the strain separation method of a kind of Xingbao mushroom according to claim 1 is characterized in that: described female kind composts or fertilisers of cultivating is peeled potatoes 180g, wheat bran 20g, agar powder 20g, glucose 20g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, water 1000ml.
5, the strain separation method of a kind of Xingbao mushroom according to claim 1 is characterized in that: described female kind composts or fertilisers of cultivating is peeled potatoes 200g, agar powder 20g, glucose 20g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 1g, water 1000ml.
6, a kind of cultivation method of bacterial strain of Xingbao mushroom is characterized in that:
A) prescription of composts or fertilisers of cultivating is: cotton seed hulls or wheat straw or straw or corncob 75~80%, wheat bran or rice bran 5~12%, the argol of poultry or livestock just 8~10%, corn flour 3~8%, the plaster of paris 1~2%, sucrose 0.5~1.5%, quicklime 0.5~1.5% adds potassium dihydrogen phosphate 0.1~0.3%, magnesium sulfate 0.1~0.2% by above-mentioned batching total amount again, each component is all percentage by weight, and pH value is 6.5~7;
B) composts or fertilisers of cultivating is made: first water was fully moistening before cotton seed hulls or wheat straw or straw or corncob crop stalk used, heap fermentation 5~7 days; Heap fermentation behind the collecting dung of poultry or livestock every turning in 3~4 days once, ferments to shakeout after 28~32 days and dries, and pulverizes standby;
Preparation method is: quicklime, the plaster of paris, potassium dihydrogen phosphate, magnesium sulfate are soluble in water, cotton seed hulls that adding ferments or wheat straw or straw or corncob are prewetted, the argol that adds rice bran, corn flour, wheat bran, sucrose and standby poultry or livestock more just uses agitator to stir 2 times, the composts or fertilisers of cultivating moisture content is controlled at 65%, the composts or fertilisers of cultivating that stirs in time branch is filled in the cultivation bag, long 17~the 20cm of cultivation bag, wide 30~33cm, cultivate material is compacting successively, and work loading height is that 22~24cm seals with cord or supporting seal mouth ring;
C) sterilization: cultivation bag is in time put into the high-pressure sterilizing pot sterilization, and 1.5 atmospheric pressure were sterilized 3 hours; Or normal-pressure sterilization, sterilized 10 hours for 100 ℃;
D) inoculation: the temperature of cultivation is inoculated in desinfection chamber or inoculating hood for 26~28 ℃, and bacterial classification must cover full composts or fertilisers of cultivating surface, and every bottle of cultivated species can be inoculated 20~30 bags;
E) mycelium management:
Postvaccinal cultivation bag moves into clean indoor, and the control temperature is sent out bacterium for 22~25 ℃, also can move into and send out bacterium in the green house of vegetables after sterilization, and control air humidity 60~63% is ventilated 2~3 every day, and each 1~2 hour, strict shading, control CO 2Concentration be 500~2000ppm, mycelium can cover with the bacterium bag in 32~35 days;
F) row's bag fruiting:
The green house of vegetables that 6m is wide is divided into 4 furrow, the wide 1.1m of every furrow, dark 0.05m, the cultivation bag of sending out bacterium good is arranged in furrow, anyhow embark on journey, near discharging, open sack between bag and the bag, with the sack warp, sack is higher than about charge level 5cm, and the control greenhouse temperature is 6~22 ℃, unified the cultivation bag of sending out well bacterium is carried out the alternating temperature stimulation, 5~7 days repeatedly, flower bud formed mushroom in a large number;
G) management of producing mushroom:
The control air humidity is sprayed clear water every day 1~2 time, keeps relative air humidity 80~90% in the mushroom canopy; Ventilate each 2 hours every day 3 times; Keep 6~22 ℃ of temperature of shed, can not surpass 22 ℃, and keep in the canopy scattered light being arranged;
H) gather:
Cap parachute-opening degree 40%, general stem grows to 5~10cm, bacteria cover diameter 2~5cm, the fruit body size is well-balanced, can gather.
7, the cultivation method of the bacterial strain of a kind of Xingbao mushroom according to claim 6, it is characterized in that: the prescription of described composts or fertilisers of cultivating is: cotton seed hulls or wheat straw or straw or corncob 78%, wheat bran or rice bran 8%, the argol of poultry or livestock just 9%, corn flour 3%, the plaster of paris 1%, sucrose 0.5%, quicklime 0.5% adds potassium dihydrogen phosphate 0.3% by above-mentioned batching total amount again, magnesium sulfate 0.1%, each component is all percentage by weight.
8, according to the cultivation method of the bacterial strain of claim 6 or 7 described a kind of Xingbao mushrooms, it is characterized in that: the ight soil of described composts or fertilisers of cultivating poultry can be the ight soil of chicken, duck or goose; The ight soil of domestic animal can be the ight soil of ox or sheep, without the ight soil of pig.
9, according to the cultivation method of the bacterial strain of claim 6 or 7 described a kind of Xingbao mushrooms, it is characterized in that: the composts or fertilisers of cultivating behind the Xinbao mushroom culturing bacterial strain on the spot also the field earth backing handle.
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