KR20020003485A - A production method of cordyceps militaris including chitosan - Google Patents

Abstract

PURPOSE: Provided is a culture method of Cordyceps militaris using a medium including chitosan. Therefore, Cordyceps militaris has anticancer activity, reinforces immunity and regulates blood sugar level. CONSTITUTION: The culture method comprises the steps of: sterilizing a culture bottle containing a medium including chitosan under high pressure; inoculating a seed inoculant the culturing it at 22-25 deg.C under artificial lightening; collecting and drying mycelia; growing them under 80-97% of humidity, 300-1200lux of artificial lightening and 18-22 deg.C of temperature; after that, stopping growing fruit bodies under 60% of humidity and 20 deg.C of temperature; and freeze-drying them.

Description

A method of growing Militaris cordyceps containing chitosan {A PRODUCTION METHOD OF CORDYCEPS MILITARIS INCLUDING CHITOSAN}

The present invention relates to a method of cultivating chitosan-containing Militaris cordyceps, more specifically, to produce a mixed medium containing chitosan after making two or more mixed medium of grain medium, animal medium, or vegetable medium. Then, the present invention relates to a method of inoculating and cultivating excellent Militaris cordyceps fungi and cultivating Militaris cordyceps fungi. Cordyceps militaris (L. ex Fr.) Link is a mushroom that forms bundles of fruiting bodies in the natural state mainly as pupa of lepidoptera insects. ~ 28㎜, cordyceps is composed of dark orange hair and a bag supporting it. Its peculiar appearance is that more than half of its shape protrudes, and its size is egg-shaped and densely distributed, and its size is 490-550㎛ × 280-350㎛. The vesicles are 400 to 420 μm × 3 to 4 μm, arranged in a line, and there are thread-shaped vesicles. Asystole consists of cells separated by a clear and clear diaphragm and develops into round secondary spores and begins to germinate. Incomplete generation of spores is consistent with the form of Verticillium.

Militaris Cordyceps sinensis with the above-mentioned characteristics is difficult to cultivate or grow the seed, conventionally a method of cultivating Militaris Cordyceps sinensis using silkworm pupa itself [Korean Patent Publication, 1999, Patent Registration No. 10- 0232710, Lee Jae-il). By the way, the present invention has the advantage that can be lowered the cost of cultivation of Cordyceps sinensis, by making a cheap medium medium, easy to obtain medium and plant medium mixed with animal medium, the production cost of Cordyceps sinensis. In addition, the addition of chitosan, which has immunity-promoting or anti-cancer effects (Progrance Journal 1995, 5. Professor, Tokura Seikichi, Hokkaido University), has the effect of further promoting excellent antibacterial, anti-cancer and immune-enhancing effects of Cordyceps sinensis itself. Its purpose is to contribute to the promotion of national health.

Therefore, the technical problem to be achieved by the present invention is to overcome the inconvenience of cultivation using a conventional silkworm pupa medium, Militaris Cordyceps sinensis using a mixed medium containing chitosan to further enhance immunity and anticancer efficacy Characterized by the mass cultivation method. More specifically, two or more types of mixed media, which can be easily learned in the surrounding natural environment, are made of a medium or animal medium, and then a mixed medium containing chitosan is produced. It is to provide a mass cultivation method of chitosan-containing Militaris cordyceps, characterized in that the inoculation culture to grow Militaris cordyceps.

In order to achieve the above object of the present invention, the present invention is a high-pressure sterilization of a culture bottle containing a medium to which chitosan is added to a mixed medium made by mixing two or more medium of the grain medium, animal medium, vegetable medium After inoculation, the seedlings were inoculated, and cultured under artificial illumination in a temperature atmosphere of 22 to 25 ° C., and then the mycelium was harvested and dried, under a humidity of 80 to 97%, artificial light 300 to 1200 Lux, and 18 to 22 ° C. After growth, when complete growth is complete, the method of cultivating chitosan-containing militaris cordyceps, which is characterized by stopping the growth of fruiting bodies at a humidity of 60% or less and a temperature atmosphere of 20 ° C or lower, and harvesting and lyophilizing the fruiting bodies. It is about. In addition, the mixed medium of claim 2, wherein one or more of the grains of brown rice, barley, soybean, corn, peanut, crude, sorghum and red beans which are available in a natural state, pupa, silkworm, slug At least one of the insects of the selected animal medium and one of the plants of herbs, lemongrass, guava, digitalis, dill, lavender, rosemary, maggiore, sage, bergamot, selfhill, chamomile, tanji, cornflower The above is characterized by producing a mixed medium made by mixing two or more medium of the selected vegetable medium. The method of claim 3, wherein the medium is not a mixed medium in which the grain medium, the vegetable medium, and the animal medium are mixed, but the medium is milled finely so that the Militaris cordyceps can easily acquire the nutrients. .

Hereinafter, specific examples will be described to help understand the present invention. However, the embodiments according to the present invention may be modified in various forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. It is intended to more fully explain the present invention to those skilled in the art.

(Example)

1. Composition of medium

At least one grain of selected grain mixture of brown rice, barley, soybeans, corn, peanuts, crude, sorghum and red beans is placed in a 600-850 ml culture bottle, followed by pupa, silkworms and slugs. After the addition of 10 to 70 g of the at least one animal mixture as it is, the herb, lemon glass, guava, digitalis, dill, lavender, rosemary, maggione, sage, bergamot, One or more of the plants of self-hill, chamomile, tanji, and cornflower are prepared in a mixed medium containing 10 to 40 g of the selected vegetable mixture as it is, followed by adding 5 to 10 g of chitosan in a small amount of 60 to 85% water in the medium. Adjust to a degree and seal. Preferably, it is more preferable to seal the culture bottle by making a mixed medium made by finely grinding the grain, animal, and vegetable mixtures, and more preferably, extracting each specific component of the grain, animal, and vegetable mixtures and extracting the mixed extract. Make a medium to which chitosan is added.

2. Sterilization

Put the culture bottle filled with the mixed medium into a high-pressure sterilization pot and autoclave for 20 to 90 minutes at 110 ~ 135 ℃.

3. Preparation of inoculum and spawn culture

After inoculating the seeds of Militaris cordyceps on PDA, YM plate medium, healthy bacteria cultured for 10 to 15 days in a thermostat are prepared as inoculators. After the prepared inoculum was placed in a shake flask and shake culture, the mycelium was crushed in a grinder and cultured in an 8-20 liter culture bottle for 6-10 days, followed by the use of spawn.

4. Inoculation

Inoculate 5 to 15 ml of the spawn seed in the prepared medium using an inoculator.

5. Culture

The inoculated culture bottles are incubated under artificial light in a sterile culture room at a temperature of 22-25 ° C. The incubation period is about 20 to 30 days, and after confirming that the mycelia in the medium is full, the culture is completed when it is full.

6. Harvesting Mycelium

When only the mycelium production is produced, the humidity is reduced to about 60%, and when the mycelium of the culture bottle is filled, the mycelium is taken out and harvested, and the harvested mycelium is put into a dryer and dried for about 24 hours in a temperature atmosphere of 40 to 60 ° C.

7. Fruiting Body Growth

The cultured medium is placed in a growth chamber under a temperature atmosphere of 18 to 22 ° C. Humidity of the growth room is maintained at 80-97%, and then irradiated at about 300-1200 Lux for artificial illumination for smooth germination of fruiting bodies. When fruiting bodies germinate, watering is first performed, and then watering is repeated every 4 to 6 days. When the germination of fruiting bodies exceeds half of the vial, the air inside the vial and the fresh air must be crossed to reduce the concentration of carbon dioxide. When the fruiting body is completely grown, the growth of fruiting body is stopped by lowering the humidity to 60% and the temperature of 20 ℃ to prevent the decay of the mycelium and fruiting body.

8. Harvesting of fruiting bodies

When fruiting is completed, the humidity is reduced to 60% and the fruiting body is taken out of the culture bottle and harvested.

9. Drying

In order to preserve the shape and color of the fruiting body, it is dried for 12 hours using a freeze dryer at -70 ℃. Thereafter, the harvested fruiting body is put into a dryer and dried under a temperature of 40 to 60 ° C. to produce and produce Cordyceps sinensis containing chitosan.

An optimal embodiment of the present invention described above has been disclosed. Although specific terms have been used herein, they are used only for the purpose of describing the present invention and are not used to limit the scope of the invention described in the claims.

According to the present invention, there is an advantage to enable mass cultivation of Militaris cordyceps using natural grains and vegetable medium, which can be easily purchased at low cost in a natural state, and recently, chitin components extracted from shellfish such as crabs and shrimps are acetylamino group. Cultivation of Militaris cordyceps using medium supplemented with chitosan, which is known as cordycepin component of Cordyceps sinensis (diabetes treatment, hypertension, strengthening of immune function, etc.) and chitosan effect (ie anti-cancer activity, strengthening of immunity) Chitosan-containing Militaris cordyceps, with the effect of blood sugar control, heavy metal adsorption and excretion, has the advantage of contributing to the improvement of national health.

Claims (3)

In the cultivation method of Militaris cordyceps, After autoclaving a culture bottle containing a medium containing chitosan to a mixed medium made by mixing two or more mediums from a grain medium, an animal medium, and a vegetable medium, inoculated with a seed, and then artificially produced in a temperature atmosphere of 22 to 25 ° C. After completion of incubation under illumination, the mycelium is harvested and dried, grown in a temperature atmosphere of 80-97% humidity, 300-1200Lux of artificial lighting, and 18-22 ° C. A method for cultivating chitosan-containing Militaris cordyceps, characterized in that the growth of fruiting bodies is stopped at 20 ° C. or lower, and fruiting bodies are harvested and lyophilized. According to claim 1, wherein the chitosan-added mixed medium is one or more of the grain medium selected from one or more of the grains of brown rice, barley, soybeans, corn, peanuts, crude, sorghum, red beans, pupa, silkworm, insects of slugs This selected animal medium and one or more of the plants of herbs, lemongrass, guava, digitaris, dill, lavender, rosemary, maggione, sage, bergamot, selfhill, chamomile, tanji and cornflower Method for cultivating chitosan-containing Militaris cordyceps, characterized in that for producing a mixed medium made by mixing two or more media in the. [Claim 3] The method for cultivating chitosan-containing militaris cordyceps, according to claim 2, wherein the chitosan-added mixture is finely pulverized to produce a mixed medium to facilitate absorption of nutrients.