KR101631926B1

KR101631926B1 - Mass Production Method of High Qulity Mushroom Mycelium Using Subculture

Info

Publication number: KR101631926B1
Application number: KR1020160017856A
Authority: KR
Inventors: 류충현
Original assignee: 농업회사법인 주식회사 류충현약용버섯
Priority date: 2016-02-16
Filing date: 2016-02-16
Publication date: 2016-06-20

Abstract

A method for mass production of high quality mushroom mycelia through the second culturing of mushroom mycelia comprises: a first step that is a medium material preparing step of preparing grains as medium materials; a second step that is a medium production step of inserting the grains into a predetermined sized plastic bag to facilitate second culturing, sealing the plastic bag, and sterilizing the grains in the plastic bag to prevent various germs from being multiplied, thereby producing a medium; a third step that is a seed inoculating step of inoculating the medium with prepared mushroom seeds after moving the produced medium to an aseptic room; a fourth step that is a first culturing step of primarily culturing the mushroom seed-inoculated medium at a predetermined temperature for a predetermined period of time; a fifth step that is a second culturing preparation step of finely breaking the medium which has been primarily cultured and in which a predetermined amount of mushroom mycelia is contained, thereby widening a space between media within the plastic bag to improve a growing environment of the mushroom mycelia such that the mushroom mycelia are grown and filled in the widened space between the media; a sixth step that is a second culturing step of performing a second culturing process by shining a light of a predetermined brightness on the mushroom mycelia to activate the mushroom mycelia; and a mushroom mycelium drying step of drying the culturing-completed mushroom mycelia such that the mushroom mycelia have a predetermined level or less of moisture. A method for mass production of high quality mushroom mycelia through second culturing of mushroom mycelia according to the present invention has enabled the mass production, has an effect of increasing production yield, and is capable of mass-producing mycelia with excellent functionality per mushroom such that the mycelia can be utilized as materials for food additives, cosmetics, and medicines and medical supplies by widening a space between the media, thereby promoting the formation of a large amount of mushroom mycelia even with the use of the same raw materials.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for mass production of high quality mushroom mycelium by secondary culture of mushroom mycelium,

The present invention relates to a method for mass production of high quality mushroom mycelium by secondary cultivation of mushroom mycelium, and more particularly, to a method for mass production of mushroom mycelium using a cereal mushroom mycelium, The present invention relates to a method for mass production of high quality mushroom mycelium through a secondary culture of mushroom mycelium developed to enable a large amount of mushroom mycelium to be formed in a space between broken cereals and grains.

The mushroom is located in the fungus of the taxonomic classification system (mushroom), and it belongs to the mushroom germ (mushroom gymnospermum), but belongs to the sericulture gymnosperm (cotyledon germ).

Mycelium (hypha, hyphae, 菌)) is very thin and thread-shaped, the mycelium is collected and the mycelium is collected and the mycelium is gathered to become the fruit body (mushroom).

In other words, these mushrooms are composed of mycelium, which is a nutrition organ, and fruiting body, which is a reproductive organ, and mycelium corresponds to roots, stems and leaves when compared with general plants, and fruiting bodies correspond to flowers.

Mycelia refers to the body of a fungus that looks like a white fluffy or thread-like fossil that is often seen when the leaves are piled up in a lush forest in summer.

Because mushrooms belong to fungi, they grow to these mycelium at first, just like other fungi. The mycelium of mushroom decomposes and absorbs the surrounding leaves, branches and other nutrients, and keeps growing. After accumulating enough nutrition, it grows up at a certain time to form a fruiting body (Carpophore).

Fruity body refers to a structure that produces and distributes spores. Therefore, in the general concept, "mushroom" means only such fruiting bodies. In academic terms, the term "mushroom mycelium" is used as the term "mushroom mycelium".

It is easy for us to think that the freshly shaped fruiting body is the whole of the mushroom, but the fact is that the occurrence of fruiting body is only for a very short period of time, and most of the year, fuzzy, thin thready mycelium is found in organic matter (Parasitic life) or infertile life (protection life) with white mold.

Mushroom is a kind of fungus that speaks of a group of umbrella-shaped needles and a sack, and is not differentiated into stems, leaves, roots, etc. like plants. In addition, there is no fibrin among cellular mutagen components but there is chitin, there is no chlorophyll, and carbon assimilation can not be done.

So they can live with other plants or absorb nutrients by decomposing the bodies of minerals and insects in plants and soil.

These fruiting fruiting bodies, which are characteristic of mushrooms, have been used as food resources since they are of concern to mankind.

However, in order to obtain such fruiting bodies (mushrooms), many processes and time are required, and cultivation techniques according to demanding growth environments are also required, and the production is shifting to production of mushroom mycelium having functionalities of each mushroom in processed and health foods and markets.

In particular, medicinal mushrooms such as mushroom, mushroom, mushroom, mushroom, mushroom, mushroom, mushroom, and bamboo are improved in cotton, blood circulation, blood sugar, antioxidant, prevention of dementia, improvement of diuretic effect. And has been known to have a variety of pharmacological functions.

The mushroom mycelium, which has the functionality of each mushroom, has a rapidly growing population of the elderly, and viral diseases have been periodically spread in the global village where we live. Due to the increase of medical expenses due to many casualties and treatment, It is expected that the number of dietary supplements has been steadily increasing due to various health food ingredients containing functional ingredients.

The method of producing mushroom mycelium by using conventional grains is simple cereal use, addition of germinated grains, functional materials, and cereal culture. However, the cultivation of mushroom mycelium using such grains can be performed by immersion, filling, sterilization cooling, seedling inoculation, In the simple mushroom cultivation method, the grains are immersed in water, and the grains are in close contact with each other. After the inoculation, the mushrooms are cultivated as much as the volume of the cereals, The amount of the mycelium to be cultivated is limited, so that there is a problem that the productivity is low in mass production of mushroom mycelium.

In addition, during the cultivation of mycelial mushroom (mycelium) and the production of mycelium, the physical shock or damage to the inoculated medium has been contraindicated in the mushroom culture until the mushroom culture period after the inoculation of the mushroom strain.

However, there is a problem that mushroom mycelium is formed only on the surface of the culture container or in a certain space inside the grain when the seed culture is left in the primary culture state after the inoculation.

1. Method for producing mushroom mycelia using germinated cereal grains and dietary fiber raw materials (Korean Unexamined Patent Application Publication No. 2003-0026140, published on March 31, 2003) 2. Description of the Related Art Korean Patent Registration No. 10-0889930 (Date of Registration: March 13, 2009) A method for mass culturing mycelia of carp mushroom and mycelia of mushroom using grains, and a method of mass culturing the mushroom mycelium Foods and methods of making them 3. Korean Patent Registration No. 10-0787170 (Date of Registration: December 12, 2007) Method for Mass Production of Mycelia of Carthamus Using Grain or Herb 4. Korean Patent Registration No. 1019960062185 (Registration Date: Jan. 16, 1999) Mass proliferation method of mushroom mycelium using a grain medium 5. A Korean Patent Registration No. 10-0383558 (registered on Apr. 28, 2003) Method for culturing liquid seeds for mass production of Cordyceps sinensis and its apparatus 6. Korean Patent Registration No. 10-1254503 (Registration date: April 09, 2013) Production method of mycelia of carrot mushroom using buckwheat

In order to solve the above-mentioned problems, the present invention uses vinyl having a volume of 3 to 4 times the amount of the curd, expands the space between the culture medium and the culture medium, and fills the mushroom mycelium in the space between the culture medium and the culture medium The present invention also provides a method for mass production of high quality mushroom mycelium by secondary cultivation of mushroom mycelium which promotes the formation of a large amount of mushroom mycelium by using the same raw materials through the cultivation process of the secondary mushroom mycelium after improving the growth environment so as to grow.

The method for producing mushroom mycelium according to the present invention comprises a first step of preparing a medium for preparing the cereal as a medium; A second step of producing a culture medium by sterilizing the cereals in a plastic bag of a predetermined size to prevent secondary growth, and preventing the growth of germs; A third step of transferring the produced medium to a clean room and inoculating the prepared mushroom seeds into the medium; A fourth step of culturing the medium inoculated with the mushroom seedlings at a predetermined temperature for a predetermined period of time; The mushroom mycelium is firstly cultured to break up the medium containing a certain amount of the medium so as to widen the space between the medium and the medium in the plastic bag so that the mushroom mycelium grows and fills in the space between the expanded medium and the medium, A fifth step of preparing a secondary culture for improvement; A sixth step of culturing the mushroom mycelium by light of a certain brightness for secondary cultivation; And drying the mushroom mycelium which has been cultivated to dry the mushroom mycelium to have a water content below a certain level.

The first step is a grain preparation step of preparing grain rice, barley, black rice or wheat; Step 1-2, which is a grain immersion step in which the mushroom seeds penetrate the grains well and the sterilization operation is facilitated, and the grains are submerged in uncontaminated groundwater for a certain time for use as a medium material; And a grain surface removing step of removing the grain surface of the grain floated by immersing the grain in which flooding has been completed in a well-drained colander, wherein the flooding time in the step 1-1 is 10 ~ 15 hours.

In the second step, the heat-resistant plastic bag having a volume of 3 to 4 times the amount of the curry and easy to sterilize can be used for the secondary culture, and the plastic bag for sealing the plastic bag, A cap which is composed of a mail cap which has an upper end of the cap inserted into the cap and a cap which covers the cap with the upper end of the cap and a cap which is sandwiched between the cap and the cap to filter out contaminated air A culture material preparing step of preparing a culture material; A step 2-2, which is a grain sealing step in which the cereal having the outer surface removed is put in the plastic bag and sealed with only the filtered air using the cap and the filter; A step 2-3 of sterilizing the grain to sterilize the germs present in the plastic bag; The sterilized cereal is cooled to 20 ~ 23 ° C until the temperature of the sterilized cereal is cooled to produce a medium, and the second stage of the production of the medium is completed. Sterilize in a high pressure sterilizer of ~ 1.5kg / ㎠G for 30 ~ 60 minutes or pasteurize at 100 ℃ for 60 ~ 100 minutes.

The above-mentioned mushroom seeds are selected from the group consisting of Phellinus linteus, Isaria japonica Yasuda, Inonotus Obliquus, Ganoderma lucidum (Curtis P. Karst.), Hericium erinaceum, Sparassis mushroom crispa Wulf.ex. Fr.) and Poria cocos (Schw.) Wolf.

The primary culturing in the fourth step is carried out until the mushroom mycelium inoculated in the medium for 15 to 20 days in a dark incubation room at 20 to 25 DEG C is cultured to the bottom of the plastic bag.

In the fifth step, in order to prevent contaminated air, which is not filtered properly during operation, from entering the plastic bag, the plastic bag immediately beneath the cap and the filter is pinched by a forceps, (5-1) which is a sealing step of a plastic bag so as not to pass through; The mushroom mycelium is firstly cultured to the bottom of the plastic bag, and the medium containing the mushroom mycelium is finely crushed to widen the space between the medium and the medium in the plastic bag so that the mushroom mycelium grows and fills in the space between the expanded medium and the medium 5-2, which is a growth environment improvement step for improving the growth environment so as to be able to improve the growth environment; And a second culture preparation step of removing the clamps sealed in the plastic bag and preparing a secondary culture.

The brightness of the illumination in the sixth step is 300 to 600 lux.

The drying method of the seventh step is lyophilization in which the mushroom mycelium is frozen and decompressed to sublimate ice to remove moisture by hot air drying at 45 to 60 ° C using a hot air dryer or using a freeze dryer.

The problems to be solved by the present invention can be solved by the mushroom mycelium production method described above.

According to the mass production method of high quality mushroom mycelium by the secondary culture of the mushroom mycelium of the present invention, the space between the medium and the medium is widened to promote mass production of mushroom mycelium by using the same raw materials, It can be used as a food additive material and a cosmetic drug substance material because it can produce a large amount of mycelial material having excellent function by mushroom.

FIG. 1 is a flow chart of a method for mass production of high quality mushroom mycelium by secondary culture of mushroom mycelium of the present invention
Fig. 2 is a photograph of the grain of the first stage according to the mass production method of the high quality mushroom mycelium by the secondary culture of the mushroom mycelium of the present invention
Fig. 3 is a photograph showing the grain submergence photograph of the step 1-2 according to the mass production method of the high quality mushroom mycelium by the secondary cultivation of the mushroom mycelium of the present invention
Fig. 4 is a photograph of a colander used for the removal of grain bins in the first to third stages according to the mass production method of high quality mushroom mycelium through the secondary culture of the mycelial mycelium of the present invention
FIG. 5 is a photograph showing the steps of the step 2-2 according to the mass production method of high-quality mushroom mycelium through the secondary cultivation of the mycelial mycelium of the present invention
6 is a perspective view of a plug and a filter used in the step 2-2 in the step of mass production of a high quality mushroom mycelium by the secondary culture of the mushroom mycelium of the present invention
FIG. 7 is a photograph of the step of sterilizing grains in step 2-3 according to the mass production method of high-quality mushroom mycelium through secondary cultivation of mycelium mycelium of the present invention
FIG. 8 is a photograph showing the steps of the grain cooling step of the second to fourth step according to the mass production method of the high quality mushroom mycelium through the secondary cultivation of the mycelial mycelium of the present invention
FIG. 9 is a photograph showing the step 3 of the inoculation step of the step 3 according to the mass production method of high quality mushroom mycelium through the secondary cultivation of the mycelial mycelium of the present invention
FIG. 10 is a photograph showing the culture medium after the first stage of the fourth step according to the mass production method of the high quality mushroom mycelium through the secondary culture of the mushroom mycelium of the present invention
11 is a photograph showing a step of improving the growth environment of step 5-2 according to the method for mass production of high quality mushroom mycelium through secondary cultivation of mushroom mycelium of the present invention
Fig. 12 is a photograph showing the secondary culture step of the sixth step according to the mass production method of high quality mushroom mycelium through the secondary cultivation of the mycelium mycelium of the present invention
13 is a photograph showing the step of drying the mycelium of mushroom in the seventh step according to the mass production method of the high quality mushroom mycelium through the secondary culture of the mushroom mycelium of the present invention
Fig. 14 is a photograph of a mushroom mycelium product produced after the completion of the seventh step according to the mass production method of high quality mushroom mycelium through the secondary cultivation of the mushroom mycelium of the present invention
FIG. 15 is a photograph of a mushroom mycelium product produced by mass production of high quality mushroom mycelium by secondary culture of mushroom mycelium of the present invention
16 is a comparative photograph 1 when the mushroom mycelium product of the present invention was produced by the mushroom mycelium product and the first culture alone
Fig. 17 is a comparative photograph 2 of the mushroom mycelium product produced by the secondary culture of the present invention and the primary culture alone

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS First, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

The term " mushroom mycelium " as used herein refers to the term " high-quality mushroom mycelium " through secondary cultivation of mushroom mycelium according to the present invention. Quot; method of mass production "in the present specification.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, a preferred embodiment of a method for mass production of high quality mushroom mycelium by secondary culture of mushroom mycelium according to the present invention will be described in detail.

The following examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention.

Fig. 1 is a flow chart of a method for mass production of high-quality mushroom mycelium by secondary cultivation of mushroom mycelium of the present invention. Fig. 2 is a graph showing the results of mass production of high quality mushroom mycelium FIG. 3 is a photograph of grain inundation in step 1 - 2 according to the mass production method of high quality mushroom mycelium through secondary cultivation of mushroom mycelium of the present invention, and FIG. 4 is a photograph showing the high quality mushroom mycelium FIG. 5 is a photograph of a colander used for the removal of grain bins in the first to third steps according to the mass production method of mycelium. FIG. FIG. 6 is a perspective view of a stopper and a filter used in the step 2-2, according to the method for mass production of high quality mushroom mycelium through the secondary culture of the mushroom mycelium of the present invention, FIG. 7 is a photograph of the grain sterilization step of the step 2-3 in accordance with the mass production method of high quality mushroom mycelium by the secondary cultivation of the mushroom mycelium of the present invention, and FIG. 8 is a photograph showing the mass of high quality mushroom mycelium 9 is a photograph of the step of inoculation of the seedling of the third step according to the mass production method of the high quality mushroom mycelium through the secondary culture of the mushroom mycelium of the present invention, FIG. 11 is a photograph of the culture medium after the first culture of the fourth step according to the mass production method of high quality mushroom mycelium through the secondary culture of the mushroom mycelium of the present invention, and FIG. 11 is a photograph of the culture medium of high quality mushroom mycelium FIG. 12 is a photograph of the step of improving the growth environment in step 5-2 according to the method of the present invention, and FIG. 12 is a photograph of the step of the second step of cultivation in the sixth step according to the method of mass production of high quality mushroom mycelium through the secondary culture of mushroom mycelium Fig. 13 is a photograph of the mushroom mycelial body drying step of the seventh step according to the mass production method of the high quality mushroom mycelium through the secondary cultivation of the mushroom mycelium of the present invention. Fig. 14 is a photograph showing the high quality mushroom mycelium Fig. 15 is a photograph of a product of mushroom mycelium produced by the mass production method of high quality mushroom mycelium through the secondary culture of the mushroom mycelium of the present invention, and Fig. Fig. 17 is a comparison photograph 1 when the mushroom mycelium product of the present invention was produced only by the secondary cultured mushroom mycelium product and the primary culture, and Fig. 17 is a photograph showing the mushroom mycelium product In the comparative photograph 2.

As shown in FIG. 1, the method for producing a mushroom mycelium according to the present invention is a method for producing mushroom mycelium by inoculating a mushroom strain with a grain as a medium, comprising the step of preparing a medium for preparing the grain as a medium A first step; A second step of producing a medium by sterilizing the grains to prevent the growth of various germs by inserting the grains into a plastic bag 3 of a predetermined size for easy secondary cultivation; A third step of transferring the produced medium to a clean room and inoculating the prepared mushroom seeds into the medium; A fourth step of culturing the medium inoculated with the mushroom seedlings at a predetermined temperature for a predetermined period of time; The mushroom mycelium is firstly cultured to break up the medium containing a certain amount of the medium so as to widen the space between the medium and the medium in the plastic bag so that the mushroom mycelium grows and fills in the space between the expanded medium and the medium, A fifth step of preparing a secondary culture for improvement; A sixth step of culturing the mushroom mycelium by light of a certain brightness for secondary cultivation; And drying the mushroom mycelium which has been cultivated to dry the mushroom mycelium to have a water content below a certain level.

The above-mentioned mushroom seeds are selected from the group consisting of Phellinus linteus, baumii, Isaria japonica Yasuda, Inonotus Obliquus, Ganoderma lucidum (Curtis P. Karst.), Hericium erinaceum, (Sparassis crispa Wulf.ex. Fr.) and Poria cocos (Schw.) Wolf.

During the cultivation of mycelium (fruiting body) or the production of mycelium, after the inoculation of the mushroom strain, the physical impact or damage to the inoculated medium has been contraindicated in the culture of the mushroom mycelium until the occurrence of the mushroom culture period. However, The method of mass production of high-quality mushroom mycelium through cultivation is a method in which a mushroom mycelium layer is formed only on a surface of a culture container or in a certain space inside a grain when the mushroom mycelium is left in a primary culture after inoculation, The mushroom mycelium broke the primary cultured medium to widen the space and at the same time regulating the mycelial growth environment for each type of mushroom, the mushroom mycelium was filled in the space expanded by the secondary mushroom mycelial growth in the secondary culture of the sixth stage This is a method to improve the growth environment in the existing production method. And the preparation step of the fifth step, the second incubation step of the production method of the sixth step is added.

The method for mass production of high quality mushroom mycelium by secondary cultivation of mushroom mycelium according to the present invention will be described below.

The first step is a medium preparation step of preparing the cereals for use as a medium, which comprises a grain preparation step of preparing brown rice, barley, black rice or wheat which are cereals; Step 1-2, which is a grain immersion step in which the mushroom seeds penetrate the grains well and the sterilization operation is facilitated, and the grains are submerged in uncontaminated groundwater for a certain time for use as a medium material; The first step is to remove the grain that has been submerged and to remove the outer surface of the grains that have been immersed in a well-drained colander.

The step 1-1 is a grain preparation step for preparing brown rice, barley, black rice or wheat for use as a nutrient required by a cultivated organism, that is, a mushroom seed, and brown rice may be used as shown in FIG.

The step 1 - 2 is a grain immersion step for immersing the cereal in uncontaminated ground water for a certain time for use as a medium for the penetration of the mushroom seeds into the grain and facilitating the sterilization work. It is preferable to flood for 10 to 15 hours by using a large-sized barrel or basin with wide-open agar made of plastic or the like.

In the step 1-3, a cereal blanket removal step for removing the grains which have been immersed in water and removing the outer surface of the flooded cereal grains in a well-drained colander is used, and a bowl having a colander or a filter net as shown in Fig. 4 is used .

In the second step, the grain is inserted into a plastic bag (3) of a predetermined size to facilitate secondary culture, and sterilized in order to prevent the growth of germs, A plastic bag 3 having a volume of 3 to 4 times the volume of the curled matter and easy to be sterilized and an upper end of the plastic bag for sealing the plastic bag so as to prevent contaminated air from entering, A cap 4 constituted by a mail cap 41 which is led in and a mail cap 42 which covers the mail cap 41 together with the upper end of the vinyl bag 3, (2) a culture material preparation step of preparing a filter (5) sandwiched between the first and second filter elements (42) so as to filter contaminated air so as not to intrude; (2-2), which is a grain filling step in which grains from which a surface is removed are placed in the plastic bag and sealed with only the filtered air using the cap (4) and the filter (5); A step 2-3 of sterilizing the cereals sterilized in order to eliminate germs present in the cereals put into the plastic bag 3; The sterilized grain is cooled to 20 ~ 23 ℃ and the grain is cooled and the medium is finished.

In the step 2-1, a plastic bag having a heat capacity of 3 to 4 times the volume of the curry and easy to be sterilized is used for the secondary culture, and the plastic bag 3 is sealed to prevent contaminated air from entering the bag. A cap (42) composed of a mail cap (41) in which an upper end of the plastic bag (3) is drawn in and a female cap (42) covering the mail cap (41) together with the upper end of the plastic bag 4), and a filter (5) sandwiched between the mail cap (41) and the female cap (42) to filter contaminated air from intrusion.

The plastic bag (3) is made of a heat-resistant polyethylene transparent plastic bag, and the mushroom mycelium is visible when cultured with the inner grain, and it is required to be able to receive a constant brightness.

As shown in FIG. 6, in order to seal the plastic bag so that contaminated air does not enter the cap, the cap has a mail cap 41 in which the upper end of the plastic bag is drawn into the inside and the upper end of the plastic bag 3, And a mail cap 42 for covering the cap 41. The filter 5 is sandwiched between the mail cap 41 and the mail cap 42 to filter out contaminated air.

The mail cap 41 is formed with an opening 411 through which the upper end portion of the plastic bag passes and passes through the lower portion of the mail cap 41. The air cap 42 is provided with a filter And a non-slippery projection 422 which is easily held by the hand when it is inserted into the mail cap 41 is formed.

It is preferable that the filter 5 is made of a special paper material having good air inflow and excellent filtration performance.

In the step 2-2, the grain is put into the plastic bag 3 with the grain removed therefrom, and the bag is sealed with the cap 4 and the filter 5 so that only the filtered air passes therethrough. 2-2-1 step of putting the grain into the plastic bag and step 2-2-2 sealing only the air filtered by using the cap 4 and the filter 5, The grains are placed in the plastic bag 3 and sealed using only the filtered air using the cap 4 and the filter 5 to complete the sealing step.

The step 2-3 is a step of sterilizing the cereals sterilized in order to eliminate the germs present in the cereals put into the plastic bag 3, Is sterilized in a high-pressure sterilizer at 120 to 125 ° C and 1.1 to 1.5 kg / cm 2 G for 30 to 60 minutes or at atmospheric pressure sterilization at 98 to 100 ° C for 60 to 100 minutes in a sterilization pot, .

In the step 2-4, the sterilized cereal is cooled until the temperature of the product is 20 ~ 23 캜, and the product is cooled and the production of the medium is completed.

The third step is a seedling step of transferring the medium produced as in FIG. 9 to a clean room and inoculating the mushroom seeds prepared in the medium.

The above-mentioned mushroom seeds are selected from the group consisting of Phellinus linteus, baumii, Isaria japonica Yasuda, Inonotus Obliquus, Ganoderma lucidum (Curtis P. Karst.), Hericium erinaceum, (Sparassis crispa Wulf.ex. Fr.) and Poria cocos (Schw.) Wolf are preferably used.

The fourth step is a primary culture step in which the medium inoculated with mushroom seeds is firstly cultured at a predetermined temperature for a certain period of time, and the primary culture in the fourth step is carried out at 20 to 25 ° C for 15 to 20 days in the medium The inoculated mushroom mycelium is cultured until the bottom of the plastic bag 3 is cultured.

10 is a photograph of a culture medium after primary culture.

Generally, 30 to 50 days are required for producing mushroom mycelium only by primary culture. However, in the primary culture of the present invention, mushroom mycelium inoculated into the culture medium for 15 to 20 days in a dark incubation room at 20 to 25 DEG C is transferred to the plastic bag 3 ) Until the bottom of the culture is cultured.

The primary culture by the above-mentioned fourth step of the present invention using a plastic bag is required to be cultivated all year round and a culture room capable of temperature control is required to produce mushrooms throughout the year. In order to maintain the temperature and humidity required for mycelial cultivation, And mycelium cultivation should be performed in a dark place without light, so a dark culture room is needed.

In the fifth step, the mushroom mycelium is firstly cultured to crush the medium containing a certain amount of the mushroom mycelium to widen the space between the medium and the medium in the plastic bag so that the mushroom mycelium grows and fills in the space between the expanded medium and the medium (4) and the filter (3) to prevent contaminated air, which is not filtered properly during operation, from entering the plastic bag (3) 5 is a sealing step of a plastic bag which does not allow the inside of the plastic bag to pass through the plastic bag immediately below the sealed portion with a forceps; The mushroom mycelium is firstly cultured to the bottom of the plastic bag (3) to break up the medium containing a certain amount of the medium, thereby widening the space between the medium and the medium in the plastic bag (3) Step 5-2, which is a growth environment improvement step for improving the growth environment so that the mycelium of mushrooms can be grown and filled; Step 5 - 3, which is a secondary culture preparation step in which the tongue in which the plastic bag 3 is closed is removed and a secondary culture is prepared.

The fifth step is a work process for expanding the area where the mycelium grows by crushing a certain amount of mushroom mycelium grown by the mycelial growth and the first culture for producing a large amount of mycelium.

The sixth step is a secondary culture step in which the culture medium having improved growth environment is cultured in a secondary culture room with illumination of constant brightness for activation of mushroom mycelium, and the brightness of the illumination is preferably 300 to 600 lux.

12 is a photograph of the secondary culture step of the sixth step.

The culture period of the sixth step is 25 to 40 days, preferably 25 to 30 days.

The culture temperature of mushroom mycelium grows at 24 ~ 26 ℃. However, the temperature in the culture medium during cultivation is 2 ~ 3 ℃ higher than the room temperature due to the hyphae of hyphae, so the culture room temperature should be kept 2 ~ 3 ℃ lower. In the latter period, it is controlled at 20 ~ 23 ℃ and cultivation is completed about 25 ~ 30 days after inoculation.

In the secondary culture, the medium that receives light is yellow and forms a vigor, so it is cultured with appropriate light.

The secondary culture requires absolutely light. The brightness of the illumination is preferably 300 to 600 lux, but it may be sufficient to produce 250 to 500 lux.

The seventh step is a step of drying the mushroom mycelium which has been cultivated so as to have a water content of less than a certain level. The drying method of the seventh step is a hot-air drying method using a hot air dryer at 45 to 60 ° C, 14 is a photograph of a mushroom mycelium produced after the completion of the seventh step, wherein the mushroom mycelium is frozen and decompressed to sublimate ice to remove water. It can be seen that the mushroom mycelium was cultured in the plastic bag 3 almost completely.

FIG. 15 is a photograph of a mushroom mycelium product produced through the above-mentioned step 7, showing that mushroom mycelium is well cultured.

Fig. 16 is a cross-sectional comparative photograph of a mushroom mycelium product produced by the secondary cultured mushroom mycelium product of the present invention and the primary culture only. Fig. 17 is a photograph showing the mushroom mycelium product of the present invention, Compared with the comparative photograph 2 in which the mushroom mycelium was produced at the time of producing the mycelium product, the secondary cultured product of the present invention was of high quality and cultured well.

The method of mass production of high quality mushroom mycelium by secondary cultivation of mushroom mycelium according to the present invention is characterized in that the mushroom mycelium mass production method comprises the steps of However, when the mushroom mycelium layer is formed only on a surface of the culture container or in a certain space inside the grain when the culture is left in the first culture state after the seedling inoculation, The mushroom mycelium broke the primary cultured medium to widen the space, and at the same time, the mycelial growth environment for each type of mushroom was controlled so that the mushroom mycelium was filled in the space expanded by the secondary mushroom mycelial growth in the secondary culture in the sixth stage A second culture preparation unit that improves the growth environment in the existing production method by growing method And the sixth step of the second cultivation step was added to the present invention, the space between the culture medium and the culture medium was widened so that mass production of mushroom mycelium was promoted by using the same raw materials, It has the effect of increasing the production yield, and it can be used as a food additive material and a cosmetic drug substance material because it can mass produce mycelium excellent in mushroom function.

1: grain 2: badge
3: plastic bag 4: cap
41: Mail Cap 411: Opening
42: Pail cap 421: Opening for ventilation
422: Non-slip projection 5: Filter

Claims

1. A method for producing mushroom mycelium by producing cereal mushroom mycelium by using cereals as a medium and inoculating mushroom seeds,
The method of producing mushroom mycelium comprises a first step of preparing a medium for preparing the cereal as a medium;
A second step of producing a culture medium by sterilizing the grains to prevent the growth of various germs by inserting the grains into a plastic bag (3) for easy culturing;
A third step of transferring the produced medium to a clean room and inoculating the prepared mushroom seeds into the medium;
A fourth step of a first culture step in which a culture medium inoculated with mushroom seeds is firstly cultured;
The first culture is carried out to break the medium containing mushroom mycelium finely so as to widen the space between the medium and the medium in the plastic bag to improve the growth environment so that the mushroom mycelium grows and fills in the space between the expanded medium and the medium. A fifth step of preparing a second culture;
A sixth step of culturing the mushroom mycelium by illuminating light for secondary activation;
Drying the mushroom mycelium which has been cultivated,
The first step is a grain preparation step of preparing grain rice, barley, black rice or wheat;
Step 1-2, which is a grain immersion step in which the grain is submerged in uncontaminated groundwater for a certain time to be used as a medium material;
And a grain removing step (step 1 - 3) for removing the surface of the grains which have been immersed in a well-drained colander by extracting the grains which have been immersed,
In the second step, the heat-resistant plastic bag having a volume of 3 to 4 times the amount of the curry and easy to sterilize can be used for the secondary culture, and the plastic bag for sealing the plastic bag, And a mail cap 42 for covering the mail cap 41 with the upper end of the vinyl bag 3 and a cap 4 for covering the mail cap 41 And a filter (5) sandwiched between the filter cap (42) and the filter cap (42) to filter contaminated air from entering the filter.
(2-2), which is a grain filling step in which grains from which a surface is removed are placed in the plastic bag and sealed with only the filtered air using the cap (4) and the filter (5);
A step 2-3 of sterilizing the cereals sterilized in order to eliminate germs present in the cereals put into the plastic bag 3;
The sterilized grains are cooled to 20 ~ 23 ℃ and then cooled to produce medium.
The immersion time in the step 1-1 is 10 to 15 hours,
The sterilization method in the step 2-3 is sterilized in a high-pressure sterilizer at 120 to 125 DEG C and 1.1 to 1.5 kg / cm < 2 > G for 30 to 60 minutes, sterilized at 100 DEG C for 60 to 100 minutes,
The above mushroom seeds use seeds of Phellinus linteus and Inonotus Obliquus,
The primary culture in the fourth step is carried out until the mushroom mycelium inoculated in the medium for 15 to 20 days in the dark incubation room at 20 to 25 ° C is cultured to the bottom of the plastic bag (3)
In the fifth step, in order to prevent contaminated air, which is not filtered properly during operation, from entering the inside of the plastic bag 3, (5-1) which is a sealing step of a plastic bag which does not allow air to pass through the bag by picking up the bag by a forceps;
The medium is firstly cultured to the bottom of the plastic bag 3 to break up the medium containing the mushroom mycelium to widen the space between the medium and the medium in the plastic bag 3 so that the mushroom mycelium 5-2, which is a growth environment improvement step for improving the growth environment so that the growth environment can be filled up;
And a second culture preparation step of removing the tongue in which the plastic bag 3 is closed and preparing a secondary culture,
The brightness of the illumination in the sixth step is 300 to 600 lux,
In the drying method of the seventh step, the mushroom mycelial body is frozen and decompressed by hot air drying at 45 to 60 ° C using a hot air drier or using a freeze drier to sublimate ice to remove moisture,
The mail cap 41 is formed with an opening 411 through which the upper end portion of the plastic bag passes and passes through the lower portion of the mail cap 41. The air cap 42 is provided with a filter A large-scale production method of high-quality mushroom mycelium by secondary cultivation of mushroom mycelium formed with an opening 421 for ventilation and an anti-slippery protrusion 422 for easy grip on the hand when it is inserted into the mail cap 41

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