CN101218876B - Producing process of termitomyces albuminosus with black skin - Google Patents
Producing process of termitomyces albuminosus with black skin Download PDFInfo
- Publication number
- CN101218876B CN101218876B CN2007101158667A CN200710115866A CN101218876B CN 101218876 B CN101218876 B CN 101218876B CN 2007101158667 A CN2007101158667 A CN 2007101158667A CN 200710115866 A CN200710115866 A CN 200710115866A CN 101218876 B CN101218876 B CN 101218876B
- Authority
- CN
- China
- Prior art keywords
- black skin
- cultivation
- termitomyces albuminosus
- mycelium
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for producing Black Tremellodon Gelatinosum belonging to the fungus cultivating field. The process of the invention includes preparation of mother culture, preparation of stock, preparation of cultivar and artificial cultivation. The invention is characterized in that the mother culture is cultivated and prepared by combining an encarpium mycelium of the Black Tremellodon Gelatinosum and a rhizoid mycelium of the Black Tremellodon Gelatinosum. Compared with the prior art, the method for producing Black Tremellodon Gelatinosum of the invention has filled the gap of the artificial cultivation technology of the Black Tremellodon Gelatinosum in the prior art and has the advantages of low cost, easy realization, etc. The invention can be broadly applied to the large-scale artificial production of the Black Tremellodon Gelatinosum.
Description
Technical field
The present invention relates to mushroom and cultivate field, specifically a kind of Termitomyces albuminosus with black skin producing process.
Background technology
Termitomyces albuminosus with black skin (Termitomyces albuminosus (Berk) Heim) belongs to Agaricales, and white mushroom section is the wild edible fungus of temperate zone and subtropical zone growth summer and autumn, collybia albuminosa a kind of.Because its activity of growing with odontoterme has substantial connection, and to dependence is strong vegetatively, does not have the precedent of artificial culture success so far.
The collybia albuminosa fine and tender taste, the smell giving off a strong fragrance, delicious flavour is one of famous wild edible mushroom, taste is best with Termitomyces albuminosus with black skin especially.The traditional Chinese medical science thinks that the flat flavor of collybia albuminosa is sweet, and strengthening the spleen and replenishing qi is arranged, and appetizing is refreshed oneself, and the effect of pain relieving oedema is the extremely desirable food of treatment hemorrhoid.Modern medicine finds that the chicken fir can also prevent intestinal cancer, bring high blood pressure down, strengthens body immunity except that hemorrhoid are had the special efficacy.The wild Termitomyces albuminosus with black skin of simple harvesting has been difficult to satisfy great demand.
Summary of the invention
Technical assignment of the present invention is at above-mentioned the deficiencies in the prior art, and a kind of Termitomyces albuminosus with black skin producing process of artificial culture Termitomyces albuminosus with black skin on a large scale is provided.
Technical assignment of the present invention is realized in the following manner: Termitomyces albuminosus with black skin producing process, comprise the preparation of female preparation of planting, original seed, preparation and the artificial cultivation that production is planted, be characterized in: the female kind by the mycelium of Termitomyces albuminosus with black skin fruit body and the rhizoid mycelium combination cultivation of Termitomyces albuminosus with black skin makes.
Described female preparation of planting may further comprise the steps:
Under a, the aseptic condition fruit body of Termitomyces albuminosus with black skin is done spore separation or separate tissue, separate the fruit body mycelium; Rhizoid to Termitomyces albuminosus with black skin is done separate tissue, separate the rhizoid mycelium;
B, the fruit body mycelium is transferred to medium is housed in vitro, cultivate down for 22～25 ℃, treat to insert the rhizoid mycelium when mycelia grows to 1.3～1.8cm, continue to cultivate and promptly get ripe female kind on the 5th～8.
In described female preparation of planting, the medium that is used to make up cultivation is:
Murphy juice 1000ml, agar 18～50g, glucose 10～30g;
Or
Jujube tree juice 1000ml, agar 18～50g, glucose 10～30g;
Or
Chinese scholartree juice 1000ml, agar 18～50g, glucose 10～30g.
Described murphy juice is the potato water extract.Potato 150～250g is put into 1000ml water heat, boil the back and continue heating 20～30 minutes, filtered while hot after adding water in the filtrate and supplying 1000ml, is put into agar, glucose.After agar, glucose melted fully, the packing test tube promptly got mother culture media.
Described jujube tree juice is jujube tree branch water extract.Jujube tree branch 180～400g is put into 1000ml water heat, boil the back and continue heating 20～30 minutes, filtered while hot after adding water in the filtrate and supplying 1000ml, is put into agar, glucose.After agar, glucose melted fully, the packing test tube promptly got mother culture media.
Described Chinese scholartree juice is Chinese scholartree branch water extract.Chinese scholartree branch 200～800g is put into 1000ml water heat, boil the back and continue heating 20～30 minutes, filtered while hot after adding water in the filtrate and supplying 1000ml, is put into agar, glucose.After agar, glucose melted fully, the packing test tube promptly got mother culture media.
Because Termitomyces albuminosus with black skin is to belong to wood destroying fungi, when the preparation of carrying out original seed, the preparation of producing kind and artificial cultivation, the medium that contains the offcuts of the lignin of high-load and agricultural byproducts in the prior art all can use.Described lignin composition is one or more the mixture in wood chip, cotton seed hulls, the corncob; The offcuts composition of agricultural byproducts is one or more the mixture in wheat bran, straw, the big soya bean stem meal.Through inventor's test of many times, the medium of preferred following prescription:
In the preparation of described original seed, culture medium prescription is:
Wood sawdust 78%, wheat bran 20%, sugar 1%, land plaster 1%, water content is 60～65%;
Or
Iblet 90%, wheat bran 5%, wood sawdust 3%, land plaster 2%, water content are 65～70%;
Or
Cotton seed hulls 78%, wheat bran 10%, wood sawdust 10%, pulverized limestone 1%, land plaster 1%,
Water content is 55～65%.
In the preparation of described production kind:
Produce to contain in kind of the medium and be not less than 90% the lignin composition and/or the offcuts composition of agricultural byproducts, water content is 50%～70%, as:
Grain 80%, wheat bran 15%, land plaster 3%, pulverized limestone 2%, water content 60%;
Or, wood sawdust 78%, wheat bran 20%, land plaster 1%, sugar 1%, water content 60%;
Or, cotton seed hulls 90%, wheat bran 5%, wood sawdust 3%, pulverized limestone 1%, land plaster 1%, water content 65%;
Or, cotton seed hulls 30%, wood sawdust 30%, corncob 30%, wheat bran 5%, land plaster 3%, pulverized limestone 2%, water content 70%.
In the described artificial cultivation process:
Contain in the composts or fertilisers of cultivating and be not less than 90% the lignin composition and/or the offcuts composition of agricultural byproducts, water content is 50%-70%.As:
Grain 80%, wheat bran 15%, land plaster 3%, pulverized limestone 2%, water content 60%;
Or, wood sawdust 78%, wheat bran 20%, land plaster 1%, sugar 1%, water content 60%;
Or, cotton seed hulls 90%, wheat bran 5%, wood sawdust 3%, pulverized limestone 1%, land plaster 1%, water content 65%;
Or, cotton seed hulls 30%, wood sawdust 30%, corncob 30%, wheat bran 5%, land plaster 3%, pulverized limestone 2%, water content 70%.
In the described artificial cultivation process, make the bacterium rod behind composts or fertilisers of cultivating pack, sterilization, the cooling inoculation, the bacterium rod places the cultivation indoor cultivation, and cultivation temperature is 22～25 ℃, is cultured to sack formation annular ring and promptly shows physiological ripening, can change the soil covering culture stage over to.
After the Termitomyces albuminosus with black skin mycelia physiological ripening, the bacterium rod removes plastic sack, earthing 5～12cm cultivation, and the cultivation temperature is 15～32 ℃, humidity 85%～95% can be gathered when the fruiting rear shank length reaches 8～16cm and be dried.
For the formation and the growth of stimulon entity better, during soil covering culture, day and night temperature should be controlled at 8～13 ℃.
During soil covering culture, soil pH value is preferably 6.0～7.5 neutrality slightly partially in the alkali scope.
Termitomyces albuminosus with black skin producing process of the present invention has been filled up the blank of relevant Termitomyces albuminosus with black skin artificial culture technology in the prior art, has following outstanding beneficial effect:
(1) realized the extensive artificial cultivation of Termitomyces albuminosus with black skin, both can reduce the open-air influence that wild environment is brought of plucking, can satisfy the great market demand of people again Termitomyces albuminosus with black skin;
(2) two parts mycelium combination is cultivated, and is the cultivation of collybia albuminosa, even cultivates for other mushroom new approaches are provided;
(3) simple, the easily realization of production process, production cost is low.
(4) edible mushrooms such as mushroom, auricularia auriculajudae, flat mushroom, Asparagus various damage by disease and insect all can occur along with increase in temperature after spring, having Termitomyces albuminosus with black skin only does not take place during the broiling summer yet, because Termitomyces albuminosus with black skin fruit body protein content is 28.6% (per 100 gram dry products), sugar part surpasses any mushroom, also have 8 kinds of necessary high-load amino acid of human body, and clear up the effect of interior rubbish of body and desinsection in addition, gnaw the cap part of this mushroom entity so up to the present only find snail.
Embodiment
With reference to following specific embodiment Termitomyces albuminosus with black skin producing process of the present invention is explained, but not as a limitation of the invention.
Embodiment 1:
1.1 female preparation (making up cultivation) of planting with Termitomyces albuminosus with black skin fruit body and the rhizoid that picked up from state, Chuxiong, Yunnan in 2004:
1.1.1 culture medium prescription:
Murphy juice 1000ml, agar 30g, glucose 20g.
1.1.2 preparation process:
A, according to above-mentioned formulated medium
Potato 200g is put into 1000ml water heat, boil the back and continue heating 20 minutes, filtered while hot after adding water in the filtrate and supplying 1000ml, is put into agar, the glucose of formula ratio.After agar, glucose melted fully, the packing test tube promptly got mother culture media;
Under b, the aseptic condition in triangular flask the fruit body to Termitomyces albuminosus with black skin do spore separation, separate the fruit body mycelium; Rhizoid to Termitomyces albuminosus with black skin is done separate tissue, separate the rhizoid mycelium;
C, the fruit body mycelium is transferred to the test tube (in 18 * 180mm) that above-mentioned medium is housed, cultivate down for 22～25 ℃, treat to insert when mycelia grows to 1.5cm (test tube medium length the 1/3) left and right sides rhizoid mycelium of mung bean grain size, similarity condition is cultivated again and was promptly obtained ripe female plant (mycelia aerial form, light gray-white) on the 7th.
1.2 the preparation of original seed
1.2.1 culture medium prescription
Wood sawdust 78%, wheat bran 20%, sugar 1%, land plaster 1%
Water content is 60～65%;
1.2.2 preparation process
After mother plants cultivation well, insert pedigree seed culture medium, both can be ripe through 30-40 days.(inoculation and cultural method are identical with conventional edible mushroom)
1.3 produce the preparation of planting
1.3.1 culture medium prescription
Cotton seed hulls 90%, wheat bran 5%, wood sawdust 3%, pulverized limestone 1%, land plaster 1%
Water content 65%
1.3.2 preparation process
Insert after original seed is cultivated and produce kind of a medium.(inoculation and cultural method are identical with conventional edible mushroom)
1.4 artificial cultivation
1.4.1 composts or fertilisers of cultivating prescription
Cotton seed hulls 90%, wheat bran 5%, wood sawdust 3%, pulverized limestone 1%, land plaster 1%
Water content 65%
1.4.2 cultivation process
(1) pack by above composts or fertilisers of cultivating prescription, is adopted 17 * 33cm * 0.05 centimeter of polyethylene plastic sack, every packed 450g;
(2) sterilization, 1.5kg pressure/cm
2Following autoclaving 2 hours is thoroughly killed assorted bacterium;
(3) inoculation makes bacterium rod (bacterium dense 15%);
(4) cultivate, the bacterium rod is moved in the culturing room, 22～25 ℃ of following lucifuges are cultivated, and after 30-40 days, are cultured to sack formation annular ring and promptly show physiological ripening;
(5) earthing is cultivated, and builds up furrow in the mushroom canopy, and the plastic sack of bacterium rod is removed, and 3～4cm at interval between bacterium rod and the bacterium rod occupies the top about 10cm of earthing in back, and soil is with the agglomerating natural soils that can loose be advisable (pH value 7.5) that fall on the ground of shaking hands.15～32 ℃ of temperature of shed, day and night temperature are controlled at about 10 ℃, and humidity remains on about 90%, the scattered beam irradiation.This mycelia does not climb the soil layer edge mycelia of keeping to the side and absorbs nutrient in the soil, and approximately earthing forms original hase and grows very long rhizoid on the bacterium rod after 30 days, and original hase top is expanded and formed the mushroom flower bud and emit unearthed face.Dry through long in time can the gathering more than 8 centimetres below 16 centimetres of about 7 days growth handle again.The 4 batches of mushrooms of gathering altogether, biology efficient reaches 80%.
Embodiment 2:
2.1 female preparation of planting (pick up from state, Chuxiong, Yunnan with the autumn in 2002, and with conventional liq paraffin be preserved in 3～5 ℃ in the refrigerator the Termitomyces albuminosus with black skin fruit body and the Termitomyces albuminosus with black skin rhizoid that picked up from state, Chuxiong, Yunnan in 2004 make up cultivation):
2.1.1 culture medium prescription:
Jujube tree juice 1000ml, agar 25g, glucose 25g
2.1.2 preparation process:
A, according to above-mentioned formulated medium
Jujube tree branch 250g is put into 1000ml water heat, boil the back and continue heating 25 minutes, filtered while hot after adding water in the filtrate and supplying 1000ml, is put into agar, glucose.After agar, glucose melted fully, the packing test tube promptly got mother culture media.；
Under b, the aseptic condition in triangular flask the fruit body to Termitomyces albuminosus with black skin do spore separation, separate the fruit body mycelium; Rhizoid to Termitomyces albuminosus with black skin is done separate tissue, separate the rhizoid mycelium;
C, the fruit body mycelium is transferred to the test tube (in 18 * 180mm) that above-mentioned medium is housed, cultivate down for 22～25 ℃, treat to insert when mycelia grows to 1.5cm (test tube medium length the 1/3) left and right sides rhizoid mycelium of mung bean grain size, similarity condition is cultivated again and was promptly obtained ripe female plant (mycelia aerial form, light gray-white) on the 7th.
2.2 the preparation of original seed
2.2.1 culture medium prescription
Cotton seed hulls 78%, wheat bran 10%, wood sawdust 10%, pulverized limestone 1%, land plaster 1%,
Water content is 60%
2.2.2 preparation process
After mother plants cultivation well, insert pedigree seed culture medium, both can be ripe through 30-40 days.(inoculation and cultural method are identical with conventional edible mushroom)
2.3 produce the preparation of planting
2.3.1 culture medium prescription
Cotton seed hulls 30%, wood sawdust 30%, corncob 30%, wheat bran 5%, land plaster 3%, pulverized limestone 2%, water content 70%.
2.3.2 preparation process
Insert after original seed is cultivated and produce kind of a medium.(inoculation and cultural method are identical with conventional edible mushroom)
2.4 artificial cultivation
2.4.1 composts or fertilisers of cultivating prescription
Grain 80%, wheat bran 15%, land plaster 3%, pulverized limestone 2%, water content 60%
2.4.2 cultivation process
(1) pack by above composts or fertilisers of cultivating prescription, is adopted 17 * 33cm * 0.05 centimetre polypropylene plastics pocket, every packed 450g;
(2) sterilization, 100 ℃ of following normal-pressure sterilizations 12 hours are thoroughly killed assorted bacterium;
(3) inoculation makes bacterium rod (bacterium dense 15%);
(4) cultivate, the bacterium rod is moved in the culturing room, 22～25 ℃ of following lucifuges are cultivated, and after 30-40 days, are cultured to sack formation annular ring and promptly show physiological ripening;
(5) earthing is cultivated, and builds up furrow in the mushroom canopy, and the plastic sack of bacterium rod is removed, and 3～4cm at interval between bacterium rod and the bacterium rod occupies the top about 10cm of earthing in back, and soil is with the agglomerating natural soils that can loose be advisable (pH value 7.5) that fall on the ground of shaking hands.15～32 ℃ of temperature of shed, day and night temperature are controlled at about 10 ℃, and humidity remains on about 90%, the scattered beam irradiation.This mycelia does not climb the soil layer edge mycelia of keeping to the side and absorbs nutrient in the soil, and approximately earthing forms original hase and grows very long rhizoid on the bacterium rod after 30 days, and original hase top is expanded and formed the mushroom flower bud and emit unearthed face.Dry through long in time can the gathering more than 8 centimetres below 16 centimetres of about 7 days growth handle again.The 4 batches of mushrooms of gathering altogether, biology efficient reaches 80%.
Claims (4)
1. Termitomyces albuminosus with black skin producing process is characterized in that, comprises the preparation of female preparation of planting, original seed, preparation and the artificial cultivation that production is planted, wherein:
The female kind by the fruit body mycelium of Termitomyces albuminosus with black skin and the rhizoid mycelium combination cultivation of Termitomyces albuminosus with black skin makes, and specifically may further comprise the steps:
Under a, the aseptic condition fruit body of Termitomyces albuminosus with black skin is done spore separation or separate tissue, separate the fruit body mycelium; Rhizoid to Termitomyces albuminosus with black skin is done separate tissue, separate the rhizoid mycelium,
B, the fruit body mycelium is transferred to medium is housed in vitro, cultivate down for 22～25 ℃, treat to insert the rhizoid mycelium when mycelia grows to 1.3～1.8cm, continue to cultivate and promptly get ripe female kind on the 5th～8;
Described medium is:
Murphy juice 1000ml, agar 18～50g, glucose 10～30g,
Or
Jujube tree juice 1000ml, agar 18～50g, glucose 10～30g,
Or
Chinese scholartree juice 1000ml, agar 18～50g, glucose 10～30g,
In the preparation of described production kind, produce to contain in kind of the medium and be not less than 90% the lignin composition and/or the offcuts composition of agricultural byproducts, water content is 50%～70%;
Described artificial cultivation process comprises pack, sterilization, inoculation, cultivation and soil covering culture, and detailed process is:
Make the bacterium rod behind a, composts or fertilisers of cultivating pack, sterilization, the cooling inoculation, the bacterium rod places the cultivation indoor cultivation, and cultivation temperature is 22～25 ℃, is cultured to sack formation annular ring and promptly shows physiological ripening, can change the soil covering culture stage over to;
After b, the Termitomyces albuminosus with black skin mycelia physiological ripening, the bacterium rod removes plastic sack, earthing 5～12cm cultivation, and the cultivation temperature is 15～32 ℃, and day and night temperature is controlled at 8～13 ℃, and humidity 85%～95% can be gathered when the fruiting rear shank length reaches 8～16cm and be dried,
Contain in the described composts or fertilisers of cultivating and be not less than 90% the lignin composition and/or the offcuts composition of agricultural byproducts, water content is 50%～70%.
2. Termitomyces albuminosus with black skin producing process according to claim 1 is characterized in that, in the preparation of described original seed, the pedigree seed culture medium prescription is:
Wood sawdust 78%, wheat bran 20%, sugar 1%, land plaster 1%, water content 60～65%;
Or
Iblet 90%, wheat bran 5%, wood sawdust 3%, land plaster 2%, water content 65～70%;
Or
Cotton seed hulls 78%, wheat bran 10%, wood sawdust 10%, pulverized limestone 1%, land plaster 1%,
Water content 55～65%.
3. Termitomyces albuminosus with black skin producing process according to claim 1 is characterized in that, produces to plant to prepare to reach in the medium in the artificial cultivation process composts or fertilisers of cultivating,
Described lignin composition is one or more the mixture in wood chip, cotton seed hulls, the corncob;
The offcuts composition of described agricultural byproducts is one or more the mixture in wheat bran, straw, the big soya bean stem meal.
4. Termitomyces albuminosus with black skin producing process according to claim 1 is characterized in that, during soil covering culture, soil pH value is 6.0～7.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101158667A CN101218876B (en) | 2007-12-26 | 2007-12-26 | Producing process of termitomyces albuminosus with black skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101158667A CN101218876B (en) | 2007-12-26 | 2007-12-26 | Producing process of termitomyces albuminosus with black skin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101218876A CN101218876A (en) | 2008-07-16 |
| CN101218876B true CN101218876B (en) | 2010-09-29 |
Family
ID=39629184
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101158667A Expired - Fee Related CN101218876B (en) | 2007-12-26 | 2007-12-26 | Producing process of termitomyces albuminosus with black skin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101218876B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110627919A (en) * | 2019-11-06 | 2019-12-31 | 安徽大学 | Intestinal prebiotics black skin termitomyces albuminosus polysaccharide ORP-1 and preparation method and application thereof |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822171A (en) * | 2010-05-24 | 2010-09-08 | 云南省农业科学院 | Artificially cultured black termitornyces albuminosus berk heim entity and culture method thereof |
| CN102487726B (en) * | 2011-12-21 | 2013-06-12 | 云南大学 | Rapid preparation method of termitomyces robustus strain |
| CN102690141B (en) * | 2012-01-15 | 2013-12-25 | 河南科技大学 | Black fungus culture medium and preparation method thereof, and method for ensuring original strain inoculation survival percent of black fungus |
| CN102599007A (en) * | 2012-04-16 | 2012-07-25 | 槐苡荃 | Artificial domestication and growing method for Termitomyces albuminosus |
| CN102726210B (en) * | 2012-05-13 | 2015-06-24 | 云南福保农业科技开发有限公司 | Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus |
| CN102701820A (en) * | 2012-05-27 | 2012-10-03 | 江苏鸿丰果蔬食品有限公司 | Termitomyces albuminosus compost and cultivation method thereof |
| CN103332996B (en) * | 2013-06-06 | 2015-08-19 | 丽水市农业科学研究院 | A kind of black fungus mother culture media and preparation method thereof plants the method for surviving rate with improving black fungus mother |
| CN103396217B (en) * | 2013-07-16 | 2015-03-04 | 邬金飞 | Formula of shiitake stock compost and preparation method of compost |
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
| CN104255297B (en) * | 2014-09-25 | 2016-05-25 | 兴义市太阳雨食用菌栽培农民专业合作社 | A kind of artificial method for planting of collybia albuminosa |
| CN104396561A (en) * | 2014-10-21 | 2015-03-11 | 云南畅佳农业科技有限公司 | Artificial cultivation method for Xerula pudens (Pers. :Fr.) sing |
| CN104885788A (en) * | 2015-06-19 | 2015-09-09 | 福建万亿店中店电子商务有限责任公司 | Black skin termitomyces albuminosus mycelium culture method |
| CN105493885B (en) * | 2015-11-24 | 2019-02-12 | 金乡县联盛菌业科技有限公司 | A kind of cultural method of Termitomyces albuminosus with black skin |
| CN105493888A (en) * | 2015-12-10 | 2016-04-20 | 何寒 | Cordyceps militaris cultured from unhulled rice grains |
| CN105532266B (en) * | 2016-02-16 | 2018-07-13 | 山东远洋农业开发有限公司 | A kind of collybia albuminosa cultural method |
| CN106069188A (en) * | 2016-06-20 | 2016-11-09 | 马碧勇 | A kind of artificial cultivation method of termitomyces |
| CN106754400A (en) * | 2016-11-22 | 2017-05-31 | 曹晓龙 | High mountain lucidum strain preparation method |
| CN106613328A (en) * | 2016-11-22 | 2017-05-10 | 曹晓龙 | Manufacture method for alpine-reishi bacteria stick |
| CN106576897A (en) * | 2016-11-22 | 2017-04-26 | 曹晓龙 | Method for planting alpine lucid ganoderma |
| CN107258327A (en) * | 2017-07-31 | 2017-10-20 | 安龙县农望种植农民专业合作社 | A kind of cultural method of collybia albuminosa |
| CN107646515A (en) * | 2017-08-30 | 2018-02-02 | 山东省科创食用菌产业技术研究院 | A kind of Termitomyces albuminosus with black skin cultural method |
| CN107801567A (en) * | 2017-11-29 | 2018-03-16 | 四川山水美地农业投资有限公司 | A kind of producing method for seed of Termitomyces albuminosus with black skin original seed |
| CN108064634A (en) * | 2017-11-29 | 2018-05-25 | 四川山水美地农业投资有限公司 | A kind of Primary spawn material of Termitomyces albuminosus with black skin and preparation method thereof |
| CN107950303A (en) * | 2017-11-29 | 2018-04-24 | 四川山水美地农业投资有限公司 | A kind of cultural method of Termitomyces albuminosus with black skin parent species |
| CN107801568A (en) * | 2017-11-29 | 2018-03-16 | 四川山水美地农业投资有限公司 | A kind of quick propagation method of Termitomyces albuminosus with black skin parent species |
| CN107896824A (en) * | 2017-11-29 | 2018-04-13 | 四川山水美地农业投资有限公司 | A kind of cultural method of Termitomyces albuminosus with black skin |
| CN108401782A (en) * | 2018-02-02 | 2018-08-17 | 金珠满江农业有限公司 | Using huge monarch's grass as black collybia albuminosa culture medium of major ingredient and the preparation method and application thereof |
| CN108307932A (en) * | 2018-03-30 | 2018-07-24 | 贺州学院 | A kind of Termitomyces albuminosus with black skin bacteria stick and its microwave sterilization method |
| CN110896924A (en) * | 2018-09-17 | 2020-03-24 | 杨易 | Artificial cultivation and planting technology for russula |
| CN109348988A (en) * | 2018-11-29 | 2019-02-19 | 广西壮族自治区烟草公司贺州市公司 | A method of cultivating casting skin chicken fir mushroom in barn |
| CN109566270A (en) * | 2018-12-28 | 2019-04-05 | 安徽省百麓现代农业科技有限公司 | A kind of Termitomyces albuminosus with black skin winter planting method |
| CN109983985A (en) * | 2019-05-12 | 2019-07-09 | 湖南省食用菌研究所 | It is a kind of to cultivate formula and preparation method thereof and cultural method by the casting skin chicken fir of main formula of reed slag |
| CN111990159B (en) * | 2020-07-03 | 2022-03-04 | 山东远洋农业开发有限公司 | Rejuvenation method of black-skin termitomyces albuminosus strain |
| CN111788990A (en) * | 2020-07-15 | 2020-10-20 | 江西鸿富食用菌研发有限公司 | White skin termitomyces albuminosus culture method |
| CN111919660A (en) * | 2020-08-11 | 2020-11-13 | 南京康之春生物科技有限公司 | Cultivation method of termitomyces albuminosus |
| CN112913578A (en) * | 2021-04-02 | 2021-06-08 | 河源市农产品流通中心有限公司 | Planting and cultivating method of black-skin termitomyces albuminosus |
-
2007
- 2007-12-26 CN CN2007101158667A patent/CN101218876B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 李林，等.鸡枞菌驯化栽培试验.食用菌 6.1995,(6),10. |
| 李林等.鸡枞菌驯化栽培试验.食用菌 6.1995,(6),10. * |
| 胡文华.鸡枞菌特征特性及栽培要点.农村经济与技术 12.1999,(12),39. |
| 胡文华.鸡枞菌特征特性及栽培要点.农村经济与技术 12.1999,(12),39. * |
| 赵守光，等.鸡枞菌引种驯化栽培试验.中国食用菌17 3.1998,17(3),11-13. |
| 赵守光等.鸡枞菌引种驯化栽培试验.中国食用菌17 3.1998,17(3),11-13. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110627919A (en) * | 2019-11-06 | 2019-12-31 | 安徽大学 | Intestinal prebiotics black skin termitomyces albuminosus polysaccharide ORP-1 and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101218876A (en) | 2008-07-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101218876B (en) | Producing process of termitomyces albuminosus with black skin | |
| CN104041330B (en) | Ganoderma tsugae imitates wild juggle cultivation method | |
| CN105493885B (en) | A kind of cultural method of Termitomyces albuminosus with black skin | |
| CN101444170B (en) | Strain separation method of apricot ormer mushroom and cultivating method thereof | |
| CN102630481B (en) | Cultivation method for oospore oudemansiella mucida | |
| CN101857494B (en) | Culture medium for pleurotus eryngii and cultivation method of pleurotus eryngii | |
| CN104285663A (en) | Black termitomyces cultivation method | |
| CN101863704B (en) | Pleurotus eryngii culture medium and culture method thereof | |
| CN101743852A (en) | Technique for cultivating high-quality and high-yield toadstool | |
| CN104920068A (en) | Dictyophora rubrovalvata cultivation method | |
| CN101822171A (en) | Artificially cultured black termitornyces albuminosus berk heim entity and culture method thereof | |
| CN103238454A (en) | Strawberry planting method | |
| CN104557244A (en) | Cultivation medium for hericium erinaceus and cultivation method of hericium erinaceus | |
| CN102557792A (en) | Culture medium of beech mushrooms and culture method of beech mushrooms | |
| CN106069188A (en) | A kind of artificial cultivation method of termitomyces | |
| CN105850502A (en) | Plateau morchella planting method | |
| CN106852252A (en) | A kind of black collybia albuminosa artificial bionic domestication and culture method | |
| CN104782407A (en) | Cultivation technology of agaricus blazei murill | |
| CN104642075A (en) | Organic ecological soilless cucumber cultivation technology in sunlight greenhouse | |
| CN103283481B (en) | A kind of pixie stool is to the exhaust processes of Pinus Massoniana Young Seedlings | |
| KR101400021B1 (en) | The method for cultivation various mushrooms using natural substances | |
| CN104429589A (en) | Method for producing monkey head mushrooms through sisal hemp waste residues | |
| CN1322801C (en) | Bacterial strain isolation and cultivation technique for Chinese wild species big fat mushroom | |
| CN106489527A (en) | Wild split the artificial field production method of lid saddle fungus | |
| CN107750851A (en) | Interplant to a kind of eggplant, tomato or green pepper the cultural method of Stropharia rugoso-annulata |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: BEIJING HESHUN ECOLOGICAL AGRICULTURE CO., LTD. Free format text: FORMER OWNER: SUN SIGUO Effective date: 20091127 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20091127 Address after: Room 4, building 1, building 18, Beiyuan Road, Beijing, Chaoyang District, 101: 100012 Applicant after: Beijing Heshun Ecological Agriculture Co., Ltd. Address before: No. 1, building No. 2, Qin Xiao street, Jinxiang County, Shandong, Jinxiang Province, Jining 16, China: 272200 Applicant before: Sun Si state Co-applicant before: Liu Hongwei |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100929 Termination date: 20121226 |