CN106754400A - High mountain lucidum strain preparation method - Google Patents

High mountain lucidum strain preparation method Download PDF

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Publication number
CN106754400A
CN106754400A CN201611029280.4A CN201611029280A CN106754400A CN 106754400 A CN106754400 A CN 106754400A CN 201611029280 A CN201611029280 A CN 201611029280A CN 106754400 A CN106754400 A CN 106754400A
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China
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jin
high mountain
days
culture medium
degrees celsius
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CN201611029280.4A
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Chinese (zh)
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曹晓龙
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Individual
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a kind of high mountain lucidum strain preparation method, 1000 milliliters of water intaking, 20 grams of agar, 20 grams of glucose stir as culture medium, ready-made culture medium is put into test tube, cooled down after high-temperature sterilization, culture medium is accessed with high mountain Wild ganoderma mycelia, grown 15 days in the environment of 15 25 degrees Celsius, obtain parent species;100 jin of chaff of maple wood, 35 jin of wheat bran, 8 jin of corn flour, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water are taken, is installed with edible mushroom special-purpose bottle after stirring, accessed with parent species after high-temperature sterilization cooling, grown 45 days in the environment of 15 25 degrees Celsius, obtain original seed;Accessed with original seed, remaining technology obtains high mountain lucidum strain as previous step.Obtain the high-quality high mountain lucidum strain of optimum large-scale plantation so that high mountain ganoderma lucidum good quality and high output.

Description

High mountain lucidum strain preparation method
Technical field
The present invention relates to a kind of ganoderma lucidum breeding method, particularly high mountain lucidum strain preparation method.
Background technology
Ganoderma lucidum is rare Chinese medicine, and high mountain ganoderma lucidum is even more the fine work in ganoderma lucidum, it is necessary to constantly explore the high-quality of high mountain ganoderma lucidum The implantation methods of high yield.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of high mountain lucidum strain preparation method.
Specifically, the technical scheme of present invention offer is:1000 milliliters of water intaking, 20 grams of agar, 20 grams of stirrings of glucose It is uniform that ready-made culture medium is put into test tube as culture medium, cooled down after high-temperature sterilization, access training with high mountain Wild ganoderma mycelia Base is supported, is grown 15 days in the environment of 15-25 degrees Celsius, obtain parent species;Take 100 jin of chaff of maple wood, 35 jin of wheat bran, corn flour 8 Jin, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water, are installed after stirring with edible mushroom special-purpose bottle, with mother after high-temperature sterilization cooling Plant and access, grown 45 days in the environment of 15-25 degrees Celsius, obtain original seed;Take 100 jin of chaff of maple wood, 35 jin of wheat bran, corn flour 8 jin, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water, installed with edible mushroom special-purpose bottle after stirring, high-temperature sterilization cooling after with original Plant and access, grown 45 days in the environment of 15-25 degrees Celsius, obtain high mountain lucidum strain.
The present invention obtain beneficial effect be:Obtain the high-quality high mountain lucidum strain of optimum large-scale plantation so that high Mountain ganoderma lucidum good quality and high output.
Brief description of the drawings
Fig. 1 is the flow chart that high mountain lucidum strain of the present invention makes.
Specific embodiment
As shown in figure 1, a kind of high mountain lucidum strain preparation method, 1000 milliliters of water intaking, 20 grams of agar, 20 grams of glucose are stirred Mix uniform as culture medium, ready-made culture medium threading test tube, cooling, is accessed with high mountain Wild ganoderma mycelia after high-temperature sterilization Culture medium, grows 15 days in the environment of 15-25 degrees Celsius, obtains parent species;Take 100 jin of chaff of maple wood, 35 jin of wheat bran, corn flour 8 jin, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water, installed with edible mushroom special-purpose bottle after stirring, high-temperature sterilization cooling after with mother Plant and access, grown 45 days in the environment of 15-25 degrees Celsius, obtain original seed;Take 100 jin of chaff of maple wood, 35 jin of wheat bran, corn flour 8 jin, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water, installed with edible mushroom special-purpose bottle after stirring, high-temperature sterilization cooling after with original Plant and access, grown 45 days in the environment of 15-25 degrees Celsius, obtain high mountain lucidum strain.Obtain optimum large-scale plantation High-quality high mountain lucidum strain so that high mountain ganoderma lucidum good quality and high output.
Specific embodiment be it is exemplary present invention is described, be not intended to limit it is of the invention be included in right will Seek interior other embodiment.

Claims (1)

1. high mountain lucidum strain preparation method, it is characterised in that:1000 milliliters of water intaking, 20 grams of agar, 20 grams of stirrings of glucose are equal It is even that ready-made culture medium is put into test tube as culture medium, cooled down after high-temperature sterilization, access culture with high mountain Wild ganoderma mycelia Base, grows 15 days in the environment of 15-25 degrees Celsius, obtains parent species;Take maple wood 100 jin of chaff, 35 jin of wheat bran, 8 jin of corn flour, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water, are installed after stirring with edible mushroom special-purpose bottle, are connect with parent species after high-temperature sterilization cooling Enter, grown 45 days in the environment of 15-25 degrees Celsius, obtain original seed;Take maple wood 100 jin of chaff, 35 jin of wheat bran, 8 jin of corn flour, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water, are installed after stirring with edible mushroom special-purpose bottle, are connect with original seed after high-temperature sterilization cooling Enter, grown 45 days in the environment of 15-25 degrees Celsius, obtain high mountain lucidum strain.
CN201611029280.4A 2016-11-22 2016-11-22 High mountain lucidum strain preparation method Pending CN106754400A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201611029280.4A CN106754400A (en) 2016-11-22 2016-11-22 High mountain lucidum strain preparation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201611029280.4A CN106754400A (en) 2016-11-22 2016-11-22 High mountain lucidum strain preparation method

Publications (1)

Publication Number Publication Date
CN106754400A true CN106754400A (en) 2017-05-31

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CN201611029280.4A Pending CN106754400A (en) 2016-11-22 2016-11-22 High mountain lucidum strain preparation method

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109906872A (en) * 2019-04-13 2019-06-21 中山市巨隆农业科技有限公司 A kind of lucidum strain production method

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1338509A (en) * 2000-08-15 2002-03-06 陈德良 Process for preparing seeds of edible and medical ectomycorrhize fungus and its antificial cuture method
CN1910988A (en) * 2006-06-05 2007-02-14 蒋中海 Method for cultivation of jiangzhonghai-China
CN101161051A (en) * 2007-11-20 2008-04-16 临漳县鑫荣珍稀食用菌有限公司 Bionic cultivation method of toadstool
CN101218876A (en) * 2007-12-26 2008-07-16 孙思国 Termitomyces albuminosus with black skin producing process
CN102823429A (en) * 2012-09-03 2012-12-19 朱斗锡 Novel morel cultivation method
CN102870595A (en) * 2012-09-13 2013-01-16 广东省微生物研究所 Artificial cultivation method capable of realizing highland cultivation of wild ganoderma tsugae murr
CN103081722A (en) * 2013-01-31 2013-05-08 吴辰 Ganoderma lucidum pictographic miniascape and cultivation method of pictographic works of art

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1338509A (en) * 2000-08-15 2002-03-06 陈德良 Process for preparing seeds of edible and medical ectomycorrhize fungus and its antificial cuture method
CN1910988A (en) * 2006-06-05 2007-02-14 蒋中海 Method for cultivation of jiangzhonghai-China
CN101161051A (en) * 2007-11-20 2008-04-16 临漳县鑫荣珍稀食用菌有限公司 Bionic cultivation method of toadstool
CN101218876A (en) * 2007-12-26 2008-07-16 孙思国 Termitomyces albuminosus with black skin producing process
CN102823429A (en) * 2012-09-03 2012-12-19 朱斗锡 Novel morel cultivation method
CN102870595A (en) * 2012-09-13 2013-01-16 广东省微生物研究所 Artificial cultivation method capable of realizing highland cultivation of wild ganoderma tsugae murr
CN103081722A (en) * 2013-01-31 2013-05-08 吴辰 Ganoderma lucidum pictographic miniascape and cultivation method of pictographic works of art

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109906872A (en) * 2019-04-13 2019-06-21 中山市巨隆农业科技有限公司 A kind of lucidum strain production method

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Application publication date: 20170531