CN101173217B - Halimasch deep-layer liquid ferment high-production method and culture medium thereof - Google Patents
Halimasch deep-layer liquid ferment high-production method and culture medium thereof Download PDFInfo
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- CN101173217B CN101173217B CN200710056167XA CN200710056167A CN101173217B CN 101173217 B CN101173217 B CN 101173217B CN 200710056167X A CN200710056167X A CN 200710056167XA CN 200710056167 A CN200710056167 A CN 200710056167A CN 101173217 B CN101173217 B CN 101173217B
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Abstract
The invention relates to a submerged liquid fermentation high-yield method and culture medium for halimasch. A halimasch strain is cultivated to a seed fungus in the culture medium of seed fungus; after being fragmentation processed, the seed fungus is inoculated by ten percents of culture medium; the cultivated conditions comprises a temperature of 24 to 28 degree centigrade, a dissolved oxygen of 20 to 40 percent, a rotational speed of 100 to 200 rpm, and the pH value of 5.5 to 6.5; the fermentation cultivate time lasts 10 to 16 days. The invention has the advantages that the culture medium made by cheap and available agricultural byproducts, such as corn powder, soybean powder, wheat bran and table cane sugar, not only decreases the production cost, but also increase the utilization value of agricultural byproducts; simultaneously, the seed fungus are fragmentally processed to produce smaller and uniform fungus balls, which improves the quality of seed fungus, speeds up the growth of the fungus ball, and greatly promotes the production as well as the fast growth of the submerged liquid fermentation of halimasch.
Description
Technical field:
The present invention relates to the fermentation culture method of honey mushroom, a kind of halimasch deep-layer liquid ferment high-production method especially is provided, also disclose the special culture media that is applicable to this method simultaneously, belong to mushroom fermentation engineering field.
Technical background:
Mushroom Chinese medicine is an important component part in the Chinese medicine treasure-house.Along with the continual renovation of development in science and technology and research means, found many secondary metabolites with pharmaceutical use in mushroom in recent years, this provides important new resources for human developing drugs.The NAOXINSHU oral liquid is made up of honey mushroom, royal jelly, honey 3 herbal medicines.Its function is strengthening by means of tonics, tranquilizing and allaying excitement.Its main ingredient honey mushroom (Armillaria mellea) belongs to Basidiomycotina, Agaricales, and white mushroom section, Armillaria is to take place in a kind of summer, autumn, energy facultative parasitism, the fungi of dietotherapeutic.Discover that in a large number honey mushroom has the similar pharmacological action of rhizoma Gastrodiae, central nervous system is had calmness and anticonvulsant action, can improve circulation of blood, increase brain and crown blood flow volume, be mainly used in headache, dizzy, neurasthenia, coronary heart disease.
Fermentation engineering is called microbial project again, is to adopt the modern project technique means, utilizes the specific function of microorganism to produce useful material, or directly microorganism is applied to industrial a kind of technology.Its advantage of liquid fermentation technology be can carry out the industrialization continuous production, scale is big, output is high, fermentation period is short, productivity effect is high.Fermentation technique plays an important role in sciemtifec and technical sphere as an importance of modern biotechnology, and along with the progress of science and technology, fermentation technique is constantly perfect, but still exists many problems---and 1. the culture medium cost of fermentation culture is higher; 2. the seed bacterium is of low quality, and is not of uniform size; 3. tunning is lower.
Summary of the invention
The purpose of this invention is to provide a kind of halimasch deep-layer liquid ferment high-production method, halimasch liquid fermentation production output is significantly improved.
The invention also discloses the substratum that is applicable to this method.
The high-yield method of honey mushroom deep fermentation of the present invention comprises the steps:
The honey mushroom bacterial classification is cultivated kind of a daughter bacteria in the seed bacterium culture medium, after will planting daughter bacteria fragmentation processing, in the 10% ratio inoculation of producing substratum, culture condition is temperature 24-28 ℃, dissolved oxygen 20-40%, rotating speed 100-200rpm, pH value 5.5-6.5, fermentation culture 10-16 days.
Comprise following ingredients in every liter of above-mentioned seed bacterium culture medium: Semen Maydis powder 20-30g, analysis for soybean powder 10-20g, edible sucrose 5-15g, ethanol 5-15g, KH
2PO
41-2g, MgSO
40.5-1.5g, pH value 5.5-6.5; To add water in the above-mentioned raw materials, boil the back and keep 10～15min, be cooled to the room temperature after-filtration, the filtrate sterilization is the seed bacterium culture medium.
Comprise following ingredients in described every liter of production substratum: Semen Maydis powder 16-22g, analysis for soybean powder 10-16g, wheat bran 18-24g, edible sucrose 14-20g, pH value 5.5-6.5; To add water in the above-mentioned raw materials, boil the back and keep 10～15min, be cooled to the room temperature after-filtration, the filtrate sterilization is the production substratum.
The present invention has the following advantages: utilize Semen Maydis powder, soyflour, wheat bran and edible sucrose to be substratum, raw material is that cost is low, the agricultural byproducts of easy acquisition, has not only reduced production cost, but also has improved the agricultural byproducts utility value; Simultaneously, kind of daughter bacteria is carried out fragmentation handles, the bacterium ball is diminished and size consistent, improved the quality of planting daughter bacteria, accelerated the bacterium ball speed of growth, make the growth of honey mushroom deep fermentation rapidly, output significantly improves.
Description of drawings:
The honey mushroom kind daughter bacteria that Fig. 1 handles without fragmentation.
Honey mushroom kind daughter bacteria after Fig. 2 fragmentation is handled.
Fig. 3 honey mushroom deep fermentation product.
Embodiment:
Embodiment 1
Seed bacterium culture medium preparation: comprise following ingredients in every liter of substratum: Semen Maydis powder 20-30g, analysis for soybean powder 10-20g, edible sucrose 5-15g, ethanol 5-15g, KH
2PO
41-2g, MgSO
40.5-1.5g, pH value 5.5-6.5.Add in 15 times of volume water, boil the back and keep 10～15min, be cooled to the room temperature after-filtration, 121 ℃ of sterilizations of filtrate, 10～15min is the seed bacterium culture medium.
Embodiment 2
Production substratum preparation: comprise following ingredients in every liter of substratum: Semen Maydis powder 16-22g, analysis for soybean powder 10-16g, wheat bran 18-24g, edible sucrose 14-20g, pH value 5.5-6.5.Add in 15 times of volume water, boil the back and keep 10～15min,, be cooled to the room temperature after-filtration, 121 ℃ of sterilizations of filtrate 15min is the production substratum.
Embodiment 3
The fragmentation of honey mushroom kind daughter bacteria is handled: after treating that kind of a daughter bacteria is grown well, pour in the homogenizer of the bacterium of going out homogenate 5min into.
Embodiment 4
Honey mushroom deep fermentation: insert the kind daughter bacteria of handling through fragmentation in 10% ratio of producing substratum, cultivated 10-16 days at temperature 24-28 ℃, dissolved oxygen 20-40%, rotating speed 100-200rpm, pH value 5.5-6.5 condition bottom fermentation.
Claims (5)
1. halimasch deep-layer liquid ferment high-production method is characterized in that may further comprise the steps:
The honey mushroom bacterial classification is cultivated kind of a daughter bacteria in the seed bacterium culture medium, after will planting daughter bacteria fragmentation processing, in the 10% ratio inoculation of producing substratum, culture condition is temperature 24-28 ℃, dissolved oxygen 20-40%, rotating speed 100-200rpm, pH value 5.5-6.5, fermentation culture 10-16 days.
2. halimasch deep-layer liquid ferment high-production method according to claim 1 is characterized in that: comprise following ingredients in every liter of seed bacterium culture medium: Semen Maydis powder 20-30g, analysis for soybean powder 10-20g, edible sucrose 5-15g, ethanol 5-15g, KH
2PO
41-2g, MgSO
40.5-1.5g, pH value 5.5-6.5; To add water in the above-mentioned raw materials, boil the back and keep 10～15min, be cooled to the room temperature after-filtration, the filtrate sterilization is the seed bacterium culture medium.
3. halimasch deep-layer liquid ferment high-production method according to claim 1 is characterized in that: comprise following ingredients in every liter of production substratum: Semen Maydis powder 16-22g, analysis for soybean powder 10-16g, wheat bran 18-24g, edible sucrose 14-20g, pH value 5.5-6.5; To add water in the above-mentioned raw materials, boil the back and keep 15min, be cooled to the room temperature after-filtration, the filtrate sterilization is the production substratum.
4. produce substratum for one kind, it is characterized in that comprising in every liter of substratum following ingredients: Semen Maydis powder 16-22g, analysis for soybean powder 10-16g, wheat bran 18-24g, edible sucrose 14-20g, pH value 5.5-6.5.
5. a seed bacterium culture medium is characterized in that comprising in every liter of substratum following ingredients: Semen Maydis powder 20-30g, analysis for soybean powder 10-20g, edible sucrose 5-15g, ethanol 5-15g, KH
2PO
41-2g, MgSO
40.5-1.5g, pH value 5.5-6.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN200710056167XA CN101173217B (en) | 2007-10-15 | 2007-10-15 | Halimasch deep-layer liquid ferment high-production method and culture medium thereof |
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| CN200710056167XA CN101173217B (en) | 2007-10-15 | 2007-10-15 | Halimasch deep-layer liquid ferment high-production method and culture medium thereof |
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| CN101173217A CN101173217A (en) | 2008-05-07 |
| CN101173217B true CN101173217B (en) | 2010-10-13 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212484B (en) * | 2011-05-05 | 2013-09-18 | 南京工业大学 | Method for controlling growthform of filamentous fungi during fermentation process |
| CN104357334B (en) * | 2014-10-24 | 2017-09-22 | 江南大学 | A kind of preparation method for the Armillaria mellea fermented solution that can improve non-enzymatic glycation inhibiting rate |
| CN104805023B (en) * | 2015-05-25 | 2018-07-27 | 北京农学院 | Halimasch microbial inoculum made of the preparation method and this method of halimasch microbial inoculum |
| CN109207380B (en) * | 2018-10-22 | 2021-08-31 | 长春圣金诺生物制药有限公司 | Liquid submerged fermentation process for high-yield armillaria mellea |
-
2007
- 2007-10-15 CN CN200710056167XA patent/CN101173217B/en active Active
Non-Patent Citations (8)
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| 李福后等.蜜环菌的研究进展.安徽农业科学35 25.2007,35(25),7741-7742. * |
| 李立恒等.蜜环菌深层发酵产多糖的研究.湖南农业大学学报（自然科学版）29 5.2003,29(5),428-430. |
| 李立恒等.蜜环菌深层发酵产多糖的研究.湖南农业大学学报(自然科学版)29 5.2003,29(5),428-430. * |
| 王怡平等.蜜环菌多糖的生物合成与发酵条件的相关性.生物学杂志17 2.2000,17(2),13-15. |
| 王怡平等.蜜环菌多糖的生物合成与发酵条件的相关性.生物学杂志17 2.2000,17(2),13-15. * |
| 邵伟等.蜜环菌深层液体发酵条件研究.中国食用菌23 3.2004,23(3),50-51. |
| 邵伟等.蜜环菌深层液体发酵条件研究.中国食用菌23 3.2004,23(3),50-51. * |
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| CN101173217A (en) | 2008-05-07 |
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