CN109906872A - A kind of lucidum strain production method - Google Patents
A kind of lucidum strain production method Download PDFInfo
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- CN109906872A CN109906872A CN201910296376.4A CN201910296376A CN109906872A CN 109906872 A CN109906872 A CN 109906872A CN 201910296376 A CN201910296376 A CN 201910296376A CN 109906872 A CN109906872 A CN 109906872A
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 14
- 239000001963 growth medium Substances 0.000 claims abstract description 14
- 229920001817 Agar Polymers 0.000 claims abstract description 10
- 239000008272 agar Substances 0.000 claims abstract description 10
- 239000012153 distilled water Substances 0.000 claims abstract description 10
- 238000011081 inoculation Methods 0.000 claims abstract description 10
- 241000894007 species Species 0.000 claims abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 7
- 239000001888 Peptone Substances 0.000 claims abstract description 7
- 108010080698 Peptones Proteins 0.000 claims abstract description 7
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 7
- 239000008103 glucose Substances 0.000 claims abstract description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 7
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 7
- 235000019319 peptone Nutrition 0.000 claims abstract description 7
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229940088594 vitamin Drugs 0.000 claims abstract description 7
- 239000011782 vitamin Substances 0.000 claims abstract description 7
- 238000000926 separation method Methods 0.000 claims abstract description 6
- 241000222336 Ganoderma Species 0.000 claims abstract description 5
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 6
- 239000010440 gypsum Substances 0.000 claims description 6
- 229910052602 gypsum Inorganic materials 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 235000013372 meat Nutrition 0.000 claims description 3
- 230000008520 organization Effects 0.000 claims description 3
- 235000013606 potato chips Nutrition 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 4
- 230000035699 permeability Effects 0.000 abstract description 2
- 240000008397 Ganoderma lucidum Species 0.000 description 8
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 6
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 2
- 208000006673 asthma Diseases 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000013220 shortness of breath Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of lucidum strain production methods, including following preparation step: (1) prepare culture medium: the culture medium includes the raw material of following parts by weight: potato 200g, agar 20g, glucose 20g, peptone 2g, multi-vitamins 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, distilled water 1000ml；(2) strain makes: comprise the following steps that: a, selection fresh glossy ganoderma carry out tissue separation；B, kind is taken；C, inoculation block is taken, is inoculated into Tube propagation base；D, it is placed in culture in biochemical cultivation case and obtains parent species；E, it takes sawdust etc. to be installed with bottle, accesses parent species, grow 45 days, obtain original seed；F, it takes sawdust etc. to be installed with bottle, accesses original seed, lower growth 45 days obtains strain.The invention has the benefit that can obtain the high-quality lucidum strain of optimum large-scale plantation using the method for the present invention, the lucidum strain cell age of culture is more consistent, and mycelia permeability is strong, growth is vibrant.
Description
Technical field
The present invention relates to strain manufacture technology field, in particular to a kind of lucidum strain production methods.
Background technique
Ganoderma lucidum is commonly called as polyporus lucidus, is the fructification of On Polyporaceae ganoderma lucidum.Ganoderma lucidum has invigorating qi for tranquilization, relieving cough and asthma
Effect has health-care effect to human body, has to disease for diseases such as dizziness egersis, shortness of breath and palpitation, neurasthenia, consumptive disease cough and asthmas
Prevent, treat or inhibit effect.The high temperatures mushroom of Ganoderma is mainly grown in relatively wet place, its entire growth and development
Ozone and the biggish growing environment of humidity are required in the process.Ganoderma lucidum has been realized in artificial greenhouseization plantation at present,
The fructification (or sclerotium) of traditional field acquisition or artificial cultivation production is rare by wild resource, fructification cultivation production is all
The conditions such as phase long (4~May), production climate control the factors such as low with bioactive substance content and restrict, to ganoderma lucidum
Mass production causes obstruction, also limits the industrialization of ganoderma lucidum.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the above technological deficiencies, provide a kind of lucidum strain production method.
In order to achieve the above object of the invention, the technical solution adopted by the present invention are as follows: a kind of lucidum strain production method, including
Following preparation step:
(1) prepare culture medium: the culture medium includes the raw material of following parts by weight: potato 200g, agar 20g, glucose
20g, peptone 2g, multi-vitamins 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, distilled water 1000ml；
Prepare culture medium the following steps are included:
A, the potato 200g for weighing peeling, slice, is placed in pot, and appropriate distilled water is added, and boils soft without rotten to potato chips；
B, with 4-6 layers of filtered through gauze, filtrate is taken；
C, agar 20g is added into filtrate, continues to boil to agar and melt completely, glucose 20g, peptone is then added
2g, multi-vitamins 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g mend distilled water to 1000 milliliters；
D, it is sub-packed in test tube while hot, is filled to the 1/5-1/4 of test tube length, clean nozzle, beyond the Great Wall lid, keep sealing；
E, then sterilize under hot conditions 40min；
F, sterilizing, which is retreaded, puts test tube, is placed at 30 DEG C and cultivates 3 days, if occurring without miscellaneous bacteria, shows sterilizing thoroughly, for inoculation
With if miscellaneous bacteria occur should sterilize or reconfigure again；
(2) strain makes: it comprises the following steps that:
A, selection button is big, just starts hacking, and fresh glossy ganoderma when fructification just occurs orange carries out tissue separation, separation
Position is selected in the intermediate position between stem base portion and cap edge；
B, meat bacteria organization first is cut with sharp aseptic operation knife when taking kind, a knife, which is cut off, separates it, never uses repeatedly
Power is torn, and injures tissue；
C, after transfer needle flame sterilization is cooling, (3-5) millimeter × (3-5) millimeter inoculation block is taken, Tube propagation base is inoculated into
In；
D, the above-mentioned culture medium for being put into inoculation block is placed in biochemical cultivation case and is cultivated, temperature adjustment is to 20-30 DEG C, culture 15
It, obtains parent species；
E, 100 jin of sawdust, 35 jin of wheat bran, 8 jin of corn flour, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water are taken, after mixing evenly
It is installed with bottle, is accessed after high-temperature sterilization is cooling with parent species, grown 45 days in the environment of 20-30 DEG C, obtain original seed；
F, 100 jin of sawdust, 35 jin of wheat bran, 8 jin of corn flour, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water are taken, after mixing evenly
It is installed with bottle, is accessed after high-temperature sterilization is cooling with original seed, grown 45 days in the environment of 20-30 DEG C, obtain strain.
The invention has the benefit that the high-quality Ganoderma Lucidum of optimum large-scale plantation can be obtained using the method for the present invention
Kind, the lucidum strain cell age of culture is more consistent, and mycelia permeability is strong, growth is vibrant.
Specific embodiment
In order that the present invention can be more clearly and readily understood, below technical solution in the embodiment of the present invention into
Row clearly and completely describes.
A kind of lucidum strain production method, including following preparation step:
(1) prepare culture medium: the culture medium includes the raw material of following parts by weight: potato 200g, agar 20g, glucose
20g, peptone 2g, multi-vitamins 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, distilled water 1000ml；
Prepare culture medium the following steps are included:
A, the potato 200g for weighing peeling, slice, is placed in pot, and appropriate distilled water is added, and boils soft without rotten to potato chips；
B, with 4-6 layers of filtered through gauze, filtrate is taken；
C, agar 20g is added into filtrate, continues to boil to agar and melt completely, glucose 20g, peptone is then added
2g, multi-vitamins 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g mend distilled water to 1000 milliliters；
D, it is sub-packed in test tube while hot, is filled to the 1/5-1/4 of test tube length, clean nozzle, beyond the Great Wall lid, keep sealing；
E, then sterilize under hot conditions 40min；
F, sterilizing, which is retreaded, puts test tube, is placed at 30 DEG C and cultivates 3 days, if occurring without miscellaneous bacteria, shows sterilizing thoroughly, for inoculation
With if miscellaneous bacteria occur should sterilize or reconfigure again；
(2) strain makes: it comprises the following steps that:
A, selection button is big, just starts hacking, and fresh glossy ganoderma when fructification just occurs orange carries out tissue separation, separation
Position is selected in the intermediate position between stem base portion and cap edge；
B, meat bacteria organization first is cut with sharp aseptic operation knife when taking kind, a knife, which is cut off, separates it, never uses repeatedly
Power is torn, and injures tissue；
C, after transfer needle flame sterilization is cooling, 3 millimeters × 3 millimeters inoculation blocks is taken, are inoculated into Tube propagation base；
D, the above-mentioned culture medium for being put into inoculation block is placed in biochemical cultivation case and is cultivated, temperature adjustment is to 20-30 DEG C, culture 15
It, obtains parent species；
E, 100 jin of sawdust, 35 jin of wheat bran, 8 jin of corn flour, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water are taken, after mixing evenly
It is installed with bottle, is accessed after high-temperature sterilization is cooling with parent species, grown 45 days in the environment of 20-30 DEG C, obtain original seed；
F, 100 jin of sawdust, 35 jin of wheat bran, 8 jin of corn flour, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water are taken, after mixing evenly
It is installed with bottle, is accessed after high-temperature sterilization is cooling with original seed, grown 45 days in the environment of 20-30 DEG C, obtain strain.
The present invention and its embodiments have been described above, this description is no restricted, only reality of the invention
One of mode is applied, actual embodiment is not limited thereto.All in all if those skilled in the art are opened by it
Show, without departing from the spirit of the invention, not inventively design embodiment similar with the technical solution,
It is within the scope of protection of the invention.
Claims (1)
1. a kind of lucidum strain production method, it is characterised in that: including following preparation step:
(1) prepare culture medium: the culture medium includes the raw material of following parts by weight: potato 200g, agar 20g, glucose 20g,
Peptone 2g, multi-vitamins 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, distilled water 1000ml；
Prepare culture medium the following steps are included:
A, the potato 200g for weighing peeling, slice, is placed in pot, and appropriate distilled water is added, and boils soft without rotten to potato chips；
B, with 4-6 layers of filtered through gauze, filtrate is taken；
C, into filtrate be added agar 20g, continue to boil to agar and melt completely, then be added glucose 20g, peptone 2g,
Multi-vitamins 2g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g mend distilled water to 1000 milliliters；
D, it is sub-packed in test tube while hot, is filled to the 1/5-1/4 of test tube length, clean nozzle, beyond the Great Wall lid, keep sealing；
E, then sterilize under hot conditions 40min；
F, sterilizing, which is retreaded, puts test tube, is placed at 30 DEG C and cultivates 3 days, if occurring without miscellaneous bacteria, shows that sterilizing thoroughly, is used for inoculation,
If miscellaneous bacteria occur should sterilize or reconfigure again；
(2) strain makes: it comprises the following steps that:
A, selection button is big, just starts hacking, fresh glossy ganoderma when fructification just occurs orange carries out tissue separation, separated part
It is selected in the intermediate position between stem base portion and cap edge；
B, meat bacteria organization first is cut with sharp aseptic operation knife when taking kind, a knife, which is cut off, separates it, never firmly tears repeatedly
It draws, injures tissue；
C, after transfer needle flame sterilization is cooling, (3-5) millimeter × (3-5) millimeter inoculation block is taken, is inoculated into Tube propagation base；
D, the above-mentioned culture medium for being put into inoculation block is placed in biochemical cultivation case and is cultivated, temperature adjustment is cultivated 15 days, obtained to 20-30 DEG C
Obtain parent species；
E, 100 jin of sawdust, 35 jin of wheat bran, 8 jin of corn flour, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water are taken, uses bottle after mixing evenly
Son installs, and is accessed after high-temperature sterilization is cooling with parent species, is grown 45 days in the environment of 20-30 DEG C, obtains original seed；
F, 100 jin of sawdust, 35 jin of wheat bran, 8 jin of corn flour, 1 jin of gypsum, 1.2 jin of white sugar, 100 jin of water are taken, uses bottle after mixing evenly
Son installs, and is accessed after high-temperature sterilization is cooling with original seed, is grown 45 days in the environment of 20-30 DEG C, obtains strain.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910296376.4A CN109906872A (en) | 2019-04-13 | 2019-04-13 | A kind of lucidum strain production method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910296376.4A CN109906872A (en) | 2019-04-13 | 2019-04-13 | A kind of lucidum strain production method |
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| CN109906872A true CN109906872A (en) | 2019-06-21 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931043A (en) * | 2019-11-26 | 2021-06-11 | 江苏红叶福茸农业科技有限公司 | A method for preparing strain of Lentinus Edodes, Hericium Erinaceus, Ganoderma, etc |
| CN115039638A (en) * | 2022-04-22 | 2022-09-13 | 云南省农业科学院生物技术与种质资源研究所 | Ganoderma resinatum strain H63 and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651568A (en) * | 2004-02-03 | 2005-08-10 | 李勇 | Edible fungus liquid culture submerged fermentation technology |
| CN106754400A (en) * | 2016-11-22 | 2017-05-31 | 曹晓龙 | High mountain lucidum strain preparation method |
| CN106754402A (en) * | 2016-11-25 | 2017-05-31 | 邓小坤 | Lucidum strain preparation method |
| CN107794226A (en) * | 2017-10-14 | 2018-03-13 | 贵州原生态生物科技有限公司 | A kind of Wild ganoderma strain selection |
-
2019
- 2019-04-13 CN CN201910296376.4A patent/CN109906872A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1651568A (en) * | 2004-02-03 | 2005-08-10 | 李勇 | Edible fungus liquid culture submerged fermentation technology |
| CN106754400A (en) * | 2016-11-22 | 2017-05-31 | 曹晓龙 | High mountain lucidum strain preparation method |
| CN106754402A (en) * | 2016-11-25 | 2017-05-31 | 邓小坤 | Lucidum strain preparation method |
| CN107794226A (en) * | 2017-10-14 | 2018-03-13 | 贵州原生态生物科技有限公司 | A kind of Wild ganoderma strain selection |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931043A (en) * | 2019-11-26 | 2021-06-11 | 江苏红叶福茸农业科技有限公司 | A method for preparing strain of Lentinus Edodes, Hericium Erinaceus, Ganoderma, etc |
| CN115039638A (en) * | 2022-04-22 | 2022-09-13 | 云南省农业科学院生物技术与种质资源研究所 | Ganoderma resinatum strain H63 and application thereof |
| CN115039638B (en) * | 2022-04-22 | 2023-12-29 | 云南省农业科学院生物技术与种质资源研究所 | Resin ganoderma lucidum strain H63 and application thereof |
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Application publication date: 20190621 |