KR100759021B1 - Method growing edible mushroom - Google Patents

Method growing edible mushroom Download PDF

Info

Publication number
KR100759021B1
KR100759021B1 KR1020060028870A KR20060028870A KR100759021B1 KR 100759021 B1 KR100759021 B1 KR 100759021B1 KR 1020060028870 A KR1020060028870 A KR 1020060028870A KR 20060028870 A KR20060028870 A KR 20060028870A KR 100759021 B1 KR100759021 B1 KR 100759021B1
Authority
KR
South Korea
Prior art keywords
sawdust
mushroom
edible mushroom
cultivation
mycelium
Prior art date
Application number
KR1020060028870A
Other languages
Korean (ko)
Inventor
여상진
Original Assignee
여상진
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 여상진 filed Critical 여상진
Priority to KR1020060028870A priority Critical patent/KR100759021B1/en
Application granted granted Critical
Publication of KR100759021B1 publication Critical patent/KR100759021B1/en

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medical Informatics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Mushroom Cultivation (AREA)

Abstract

A method for growing edible mushroom is provided to cultivate Phellinus linteus or Sparassis Crispa in material lumber or sawdust, and mix the material lumber or sawdust and a hypha extract with a culture medium culturing the edible mushroom such as agaric, Pleurotus eryngii, and Flammulina velutipes. The beta-glucan activates the immunity of cell tissue and inhibits the proliferation and recurrence of cancer cells. A method for growing edible mushroom comprises the steps of: preparing a by-product by pulverizing material lumber into sawdust in case of cultivating medicinal mushroom in the material lumber, or by removing sawdust from a culture bottle and sorting out uniform sawdust in case of cultivating the medicinal mushroom in the sawdust culture medium; preparing a hypha extract by adding wheat bran containing saccharide into a stainless container, sterilizing it, inoculating medicinal mushroom spawn, culturing the mushroom spawn partially, and extracting the hypha; and growing edible mushroom by mixing the sawdust formed in the first step and the hypha extract formed in the second step with culture medium sawdust, putting the mixture in a culture bottle, sterilizing it in a sterilizer, cooling it in an aseptic room, inoculating edible mushroom spawn on it, culturing the edible mushroom spawn in a culture room, and then growing the edible mushroom in a cultivation room.

Description

식용버섯 재배방법{METHOD GROWING EDIBLE MUSHROOM}Cultivation of Edible Mushrooms {METHOD GROWING EDIBLE MUSHROOM}

도 1 은 본 발명의 일 실시예에 따른 식용버섯 재배방법의 순서도1 is a flow chart of the edible mushroom cultivation method according to an embodiment of the present invention

본 발명은 식용버섯의 재배방법에 관한 것으로, 더욱 상세하게는 베타글루칸 성분이 함유되어 있는 상황버섯 또는 꽃송이버섯을 재배한 원목 또는 톱밥과 균사체의 액기스를 느타리버섯, 새송이버섯, 팽이버섯 등의 식용버섯을 배양하는 배지에 혼합하여 베타글루칸 성분이 함유된 식용버섯을 재배함으로써 저렴한 가격에 면역력 증강과 항암효과를 발휘하는 버섯을 재배할 수 있도록 한 식용버섯 재배방법에 관한 것이다.The present invention relates to a cultivation method of edible mushrooms, and more specifically, the extract of raw wood or sawdust and mycelium cultivated situation mushrooms or blossom mushrooms containing beta glucan component edible mushrooms, such as oyster mushroom, matsutake mushroom, enoki mushroom By cultivating an edible mushroom containing beta glucan component by mixing the mushroom culture medium, it relates to an edible mushroom cultivation method that can be grown mushrooms exhibiting enhanced immunity and anticancer effect at a low price.

일반적으로 버섯은 버섯에 함유된 독특한 약리성분으로 인하여 예로부터 극동 아시아 지역에서는 버섯을 약재로 이용한 한방 및 민간요법이 지속적으로 전승되어 왔으며, 버섯에 함유된 약리성분이 생체방어, 생체항상성 유지, 체내리듬의 조절, 질병회복 등에 효과적일 뿐만 아니라, 암, 뇌졸증, 심장병 등의 성인병에 대 한 예방과 개선효과에도 유효함이 보고되고 있다.In general, mushrooms have traditionally been passed on oriental medicine and folk remedies using mushrooms as a medicinal herb in the Far East Asia region, due to the unique pharmacological ingredients contained in mushrooms. Not only is it effective in regulating rhythms and recovering diseases, it is also reported to be effective in preventing and improving the effects of adult diseases such as cancer, stroke and heart disease.

이러한 약리작용을 포함하는 약용버섯에는 대표적으로 상황버섯, 꽃송이버섯 등이 있는데, 상황버섯, 꽃송이버섯 등에 함유되어 있다고 알려진 항암성분은 주로 베타글루칸에 의한 면역기능 향상 효과에 기인하는 것이다.Medicinal mushrooms containing such pharmacological action are typically mushrooms, matsutake mushrooms, etc. The anti-cancer ingredients known to be contained in situation mushrooms, matsutake mushrooms are mainly due to the effect of improving immune function by beta glucan.

상황버섯의 모양은 처음에는 진흙 덩어리가 뭉쳐진 것 같은 형태로 유지되다가 다 자란 후에는 마치 나무 그루터기에 혓바닥을 내밀고 있는 모습이어서 수설이라고도 하며, 혓바닥 같은 형태의 윗부분이 진흙과 같은 색깔을 띠기도 하고, 감나무의 표피와 같이 검게 나타나기도 하며 아랫부분은 노란 융단 같은 형태를 띤다.The shape of the mushrooms is maintained in the form of a mass of mud at first, but after it is grown, it is like a snow tree because it is sticking to the tongue on a tree stump, and the upper part of the tongue is shaped like mud. It appears black like the skin of persimmon, and the lower part is shaped like a yellow carpet.

또한, 꽃송이버섯은 가을에 침엽수의 자른 그루터기나 고목의 언저리에서 자생한다. 자실체는 높이 10∼25cm로 육질이고 밑부분은 굵은 줄기로 공통의 자루가 되어 있으며, 여러 차례 가지를 쳐서 꼭대기는 편평하게 되고 그 가장자리가 물결 모양이다. 자실층은 꽃잎 뒷면에 발달하였다. 자루는 길이 2∼5cm, 나비 2∼4cm로 짧고 뭉툭하며 단단하며 조직은 흰색이다. 포자는 길이 5∼7㎛, 나비 3∼5㎛로 달걀 모양 또는 타원형이고 표면은 평편하며 포자무늬가 흰색인 식용버섯으로 한국·일본·유럽·북아메리카·오스트레일리아 등지에 분포한다In addition, blossom mushrooms grow on cut stumps of coniferous trees or on the edges of old trees in autumn. Fruiting bodies are fleshy, 10-25cm in height, and the bottom part is a common sack with thick stems. The branches are flat several times and the edges are wavy. The fruiting layer developed behind the petals. Sack is 2-5cm long, 2-4cm long, blunt, hard, and tissue is white. Spore 5 ~ 7㎛ long, 3 ~ 5㎛ butterfly, egg-shaped or oval, flat surface, white spore pattern, edible mushroom, distributed in Korea, Japan, Europe, North America, Australia, etc.

이러한 상황버섯이나 꽃송이버섯에 함유되어 있는 베타글루칸이란, 포도당(glucose)이 β-1,3 화학결합을 중심으로 중합된 다당류를 총칭하는 말로 버섯, 효모 등 미생물로부터 분리, 생산되는 미생물 유래의 베타글루칸과 곡물의 식이섬유에서 추출 생산되는 식물성 베타글루칸이 있다.Beta-glucan, which is contained in such situation mushrooms and flower mushrooms, is a generic term for polysaccharides in which glucose is polymerized around β-1,3 chemical bonds, and is derived from microorganisms, such as mushrooms and yeasts. Vegetable beta glucan is extracted from glucan and grain fiber.

이러한 베타글루칸(β-Glucan)은 인간의 정상적인 세포조직의 면역기능을 활 성화시켜 암세포의 증식과 재발을 억제하고 면역세포의 기능을 활발하게 하는 인터루킨(interleukin), 인터페론(interferon)의 생성을 촉진시킨다. Β-Glucan activates the immune function of human normal cellular tissues, inhibits the proliferation and recurrence of cancer cells, and promotes the production of interleukin and interferon that activate immune cell functions. Let's do it.

또한, 활성 베타글루칸은 암세포가 있는 체내로 들어가 사이토카인(Cytokine)을 생산시킴으로써 면역 세포인 T세포와 B세포의 활동을 지원하여 세포조직의 면역기능을 활성화시킨다. In addition, active beta glucan enters the body with cancer cells to produce cytokines (Cytokine) to support the activity of immune cells T cells and B cells to activate the immune function of cellular tissues.

이러한 미국, 일본 등 전 세계적으로 기능성 식품 및 화장품 등으로 널리 이용되고 있으나, 자연산의 채취량이 매우 작아 가격이 비싸고, 생육조건이 까다롭고 수년 혹은 수십년이 지나야 수확할 수 있을 정도로 성장속도가 매우 느려 재배하는데 많은 어려움이 있었다.It is widely used as a functional food and cosmetics all over the world such as the United States and Japan, but it is very expensive because of the small amount of natural extracts, the growing conditions are difficult, and the growth rate is very slow to be harvested after several years or decades. There were many difficulties in cultivation.

따라서, 본 발명의 목적은 베타글루칸 성분이 함유되어 있는 상황버섯 또는 꽃송이버섯을 재배한 원목 또는 톱밥과 균사체의 액기스를 느타리버섯, 새송이버섯, 팽이버섯 등의 식용버섯을 배양하는 배지에 혼합하여 베타글루칸 성분이 함유된 식용버섯을 재배함으로써 저렴한 가격에 면역력 증강과 항암효과를 발휘하는 식용버섯을 재배할 수 있도록 한 식용버섯 재배방법을 제공하는 것이다.Accordingly, an object of the present invention is to mix the extract of the wood or sawdust and mycelium cultivated situation mushrooms or matsutake mushrooms containing beta glucan components in the medium for cultivating edible mushrooms such as oyster mushrooms, matsutake mushrooms, enoki mushrooms and beta By cultivating edible mushrooms containing glucan components, it is to provide an edible mushroom cultivation method that enables cultivated edible mushrooms that exhibit immunity and anticancer effects at an affordable price.

전술한 본 발명의 목적은, 약용버섯을 원목에 재배하는 경우 그 원목을 톱밥으로 분쇄하거나, 약용버섯을 톱밥 배지에 재배하는 경우, 그 톱밥을 탈병하여 균 일한 톱밥을 선별하는 부산물 준비단계와, 스테인리스 용기에 당 성분이 함유된 밀기울을 첨가하여 살균한 후, 약용버섯의 종균을 접종하여 일부 배양시켜 액기스화하는 균사체 준비단계와, 상기 부산물 준비단계에서 형성된 톱밥과 상기 균사체 준비단계에서 형성된 균사체 액기스와 배지용 톱밥을 혼합하여 배양병에 입병하여 살균기 내에서 살균한 후, 무균실 내에서 냉각시킨 다음, 식용버섯의 종균을 접종하여 배양실에서 배양하며, 배양이 완료된 다음 재배실에서 재배하는 식용버섯 재배단계를 포함하는 것을 특징으로 하는 식용버섯 재배방법을 제공함에 의해 달성된다.The above-described object of the present invention, when the medicinal mushrooms are cultivated in solid wood, the wood is crushed with sawdust, or when the medicinal mushrooms are grown in sawdust medium, by-product preparation step of selecting the uniform sawdust by degenerating the sawdust, After sterilizing by adding the bran containing sugar components to the stainless steel container, the mycelium preparation step of inoculating and incubating the spawn of medicinal mushroom spawn part, the sawdust formed in the by-product preparation step and the mycelium solution formed in the mycelium preparation step And sawdust for medium and mixed into a culture bottle, sterilized in a sterilizer, cooled in a sterile chamber, inoculated with seedlings of edible mushrooms, cultured in a culture room, and grown in an cultivation room after cultivation of edible mushrooms. It is achieved by providing an edible mushroom cultivation method comprising the step.

본 발명의 바람직한 특징에 따르면, 전술한 식용버섯 재배단계에서 재배실의 습도를 조절하는 가습수에는 상기 균사체 준비단계에서 형성된 균사체 액기스 3%가 포함된 것으로 한다.According to a preferred feature of the present invention, the humidified water for controlling the humidity of the cultivation room in the above-mentioned edible mushroom cultivation step is to contain 3% mycelium extract formed in the mycelium preparation step.

본 발명의 더 바람직한 특징에 따르면, 전술한 식용버섯 재배단계에서의 배양병 입병 비율은, 상기 부산물 준비단계에서 형성된 톱밥 20중량%와, 상기 균사체 준비단계에서 형성된 균사체 액기스 20중량%와 배지용 톱밥 60중량%인 것으로 한다.According to a more preferred feature of the invention, the above-mentioned cultivation ratio of the cultivation mushroom in the above-mentioned edible mushroom cultivation step, 20% by weight of the sawdust formed in the byproduct preparation step, 20% by weight of the mycelium solution formed in the mycelium preparation step and sawdust for medium It shall be 60 weight%.

본 발명의 더욱 바람직한 특징에 따르면, 전술한 약용버섯은 상황버섯 또는 꽃송이버섯인 것으로 한다.According to a more preferable feature of the present invention, the above-mentioned medicinal mushroom is to be a situation mushroom or blossom mushroom.

이하에는 본 발명의 바람직한 실시예를 첨부도면을 참조하여 상세하게 설명하되, 이는 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 발명을 용이 하게 실시할 수 있을 정도로 상세하게 설명하기 위한 것이지, 이로 인해 본 발명의 기술적인 사상 및 범주가 한정되는 것을 의미하지는 않는다.DETAILED DESCRIPTION Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, which are intended to explain in detail enough to enable those skilled in the art to easily carry out the invention. This does not mean that the technical spirit and scope of the present invention is limited.

도 1 은 본 발명의 일 실시예에 따른 식용버섯 재배방법의 순서도가 도시된다.1 is a flow chart of the edible mushroom cultivation method according to an embodiment of the present invention.

본 발명의 일 실시예에 따른 식용버섯 재배방법은, 약용버섯을 원목에 재배하는 경우 그 원목을 톱밥으로 분쇄하거나, 약용버섯을 톱밥 배지에 재배하는 경우, 그 톱밥을 탈병하여 균일한 톱밥을 선별하는 부산물 준비단계와, 스테인리스 용기에 당 성분이 함유된 밀기울을 첨가하여 살균한 후, 약용버섯의 종균을 접종하여 일부 배양시켜 액기스화하는 균사체 준비단계와, 상기 부산물 준비단계에서 형성된 톱밥과 상기 균사체 준비단계에서 형성된 균사체 액기스와 배지용 톱밥을 혼합하여 배양병에 입병하여 살균기 내에서 살균한 후, 무균실 내에서 냉각시킨 다음, 식용버섯의 종균을 접종하여 배양실에서 배양하며, 배양이 완료된 다음 재배실에서 재배하는 식용버섯 재배단계를 포함한다.In the edible mushroom cultivation method according to an embodiment of the present invention, when medicinal mushrooms are cultivated on wood, the wood is crushed with sawdust, or when medicinal mushrooms are cultivated on sawdust medium, the sawdust is degenerated to select uniform sawdust. The by-product preparation step and the sterilization by adding the bran containing the sugar component to the stainless steel container, and then inoculated with the seed of the medicinal mushroom to prepare the mycelium to be liquefied by part, and the sawdust and the mycelium formed in the by-product preparation step After mixing the mycelium solution formed in the preparation step and the sawdust for the medium and enter the culture bottle to sterilize in the sterilizer, cooled in a sterile chamber, inoculated with the spawn of edible mushrooms and incubated in the culture room, the culture room is completed Contains edible mushroom cultivation step grown in.

여기서 부산물 준비단계는 상황버섯, 꽃송이버섯 등의 약용버섯이 재배되었던 원목을 톱밥으로 분쇄하거나 톱밥 배지를 탈병하여 균일한 톱밥으로 선별하는 단계이다.Here, the by-product preparation step is a step of crushing solid wood, which was grown medicinal mushrooms, such as edible mushrooms, matsutake mushrooms with sawdust or screening the sawdust medium with uniform sawdust.

전술한 부산물은 약용버섯에 함유되어 있는 베타글루칸 성분을 함유하고 있어 식용버섯의 종균이 접종되는 배양병에 혼합시켜 식용버섯의 베타글루칸 형성을 증진시키는 역할을 한다The above by-products contain the components of beta glucan contained in medicinal mushrooms, which are mixed with a culture bottle in which the seeds of edible mushrooms are inoculated, thereby promoting the formation of beta glucans of edible mushrooms.

균사체 액기스 준비단계는 상황버섯, 꽃송이버섯 등의 약용버섯의 종균을 접종한 후 배양하여 액기스화 하는 단계로, 식용버섯의 종균이 접종되는 배양병에 혼합되거나 식용버섯의 종균이 배양된 후 재배과정에서 가습수에 혼합되어 습도조절용으로 사용함으로써 식용버섯의 베타글루칸 함량을 배가시키도록 한다.The mycelial extract preparation step is inoculation by inoculation after inoculation of the spawn of medicinal mushrooms such as edible mushrooms and zinnia mushroom, which is mixed with a culture bottle in which the spawn of edible mushrooms is inoculated or cultivated after the spawn of edible mushrooms is incubated. It is mixed with humidified water at and used to control humidity to double the content of beta glucan of edible mushrooms.

식용버섯 재배단계에서는 전술한 부산물 준비단계에서 형성된 부산물과, 균사체 액기스 준비단계에서 형성된 균사체 액기스를 배지용 톱밥과 일정 비율로 혼합하여 배양병에 입병한 후 살균, 냉각, 배양 과정을 거쳐서 배양하는 단계이다.In the edible mushroom cultivation step, the by-product formed in the above-mentioned by-product preparation step and the mycelium extract formed in the mycelium extract preparation step are mixed with a sawdust for medium at a predetermined ratio, and then introduced into a culture bottle, followed by culturing through sterilization, cooling, and culturing. to be.

전술한 식용버섯 재배단계에서는 전술한 부산물과 균사체 액기스로 인해 식용버섯 내에 베타글루칸을 형성시킴으로써 식용버섯의 섭취만으로도 면역기능 향상과 항암효과를 발휘할 수 있도록 한다.In the above-mentioned edible mushroom cultivation step, beta glucan is formed in the edible mushroom due to the above-mentioned by-products and mycelium extracts, so that the intake of edible mushrooms can improve immune function and anticancer effect.

또한, 전술한 식용버섯 재배단계에서 재배되는 느타리버섯, 새송이버섯, 팽이버섯 등의 식용버섯은 그 종류에 따라 배양일수, 배양온도, 습도 등을 달리하여 재배한다.In addition, edible mushrooms such as oyster mushroom, matsutake mushroom, enoki mushroom, which are cultivated in the above-mentioned edible mushroom cultivation step, are grown by varying the number of days of culture, cultivation temperature, humidity, etc. according to the type.

이하에는, 도 1 에 도시되는 바와 같이, 본 발명을 실시예에 의거 더욱 상세하게 설명하면 다음과 같다Hereinafter, as shown in FIG. 1, the present invention will be described in more detail based on Examples.

실시예 ;Example;

상황버섯이 원목재배된 경우, 그 원목을 톱밥으로 분쇄하고, 톱밥 배지에서 재배된 경우, 그 톱밥을 균일하게 탈병하여 부산물을 형성한다.Situation mushrooms are cultivated with wood, the wood is crushed with sawdust, and when grown in sawdust medium, the sawdust is uniformly demineralized to form by-products.

전술한 부산물 형성과는 별도로, 150 ~ 300L 스테인리스 용기에 당 성분이 함유된 밀기울을 첨가하여 120℃의 온도로 고압살균 한 뒤, 상황버섯 종균을 접종하여 배양한다.Apart from the above by-product formation, the bran-containing sugar is added to a 150 ~ 300L stainless steel container, autoclaved at a temperature of 120 ℃, and then incubated by incubating the mushrooms spawn.

이때, 배양일은 15일 전후로 배양하고, 배양된 균사체는 액기스화 하여 균사체 액기스를 형성한다.At this time, the culture day is cultured around 15 days, the cultured mycelium is formed to form a mycelium extract.

전술한 부산물과 균사체 액기스는 배지용 톱밥과 혼합하여 배양병에 입병시키는데, 부산물, 균사체 액기스 및 비지용 톱밥의 비율은 각각 20중량%, 20중량% 및 60중량% 인 것이 바람직하다.The above-mentioned by-products and mycelium extracts are mixed with the sawdust for the medium and put into the culture bottle. The ratio of the by-products, mycelium extracts and the sawdust for busy hands is preferably 20% by weight, 20% by weight and 60% by weight, respectively.

전술한 비율로 입병 된 배양병은 살균솥 또는 멸균기 내에서 살균처리 되는데, 고압살균의 경우 115℃ ~ 120℃ 의 온도에서 살균하며, 상압살균의 경우 98℃ ~ 100℃ 의 온도에서 살균하는 것이 바람직하다.Culture bottles bottled at the above-mentioned ratios are sterilized in a sterilization pot or sterilizer. In the case of autoclaving, sterilization is performed at a temperature of 115 ° C. to 120 ° C., and in the case of atmospheric sterilization, sterilization is performed at a temperature of 98 ° C. to 100 ° C. Do.

살균처리된 배양병은 무균실에서 냉각처리된 후, 느타리버섯, 새송이버섯, 팽이버섯 등의 식용버섯 종균을 접종하여 배양실에서 배양한다.The sterilized culture bottles are cooled in a sterile chamber, and then inoculated with edible mushroom spawns such as oyster mushroom, matsutake mushroom, and enoki mushroom and incubated in the culture chamber.

이때, 배양실 온도는 21℃ ~ 24℃ 인 것이 바람직하며, 느타리버섯의 경우 20일 전후, 새송이버섯의 경우 30일 전후, 팽이버섯의 경우 27일 전후로 배양한다.At this time, the culture room temperature is preferably 21 ℃ ~ 24 ℃, about 20 days for the oyster mushroom, 30 days before and after the mushrooms, cultivated around 27 days for the mushrooms.

배양이 완료된 종균은 재배실로 옮겨져 재배되며, 버섯의 종류에 따라 온도 및 습도 조절을 하는데, 습도조절의 경우 전술한 균사체 액기스 준비단계에서 형성된 균사체 액기스를 가습수에 3중량% 혼합하여 가습해 줌으로써 식용버섯의 베타글루칸 형성을 증가시킨다.After completion of the cultivation, the spawn is transferred to the cultivation room and cultivated, and temperature and humidity are controlled according to the type of mushroom. In the case of humidity control, the mycelium extract formed in the above-described preparation of mycelium extract is humidified by mixing 3% by weight of the mycelium extract with humidified water. Increases beta glucan formation in mushrooms.

이상에서와 같이, 본 발명에 따른 식용버섯 재배방법은, 베타글루칸 성분이 함유되어 있는 상황버섯 또는 꽃송이버섯을 재배한 원목 또는 톱밥과 균사체의 액기스를 느타리버섯, 새송이버섯, 팽이버섯 등의 식용버섯을 배양하는 배지에 혼합하여 베타글루칸 성분이 함유된 식용버섯을 재배함으로써 저렴한 가격에 면역력 증강과 항암효과를 발휘하는 식용버섯을 재배할 수 있는 효과가 있다.As described above, the edible mushroom cultivation method according to the present invention, edible mushrooms, such as oyster mushroom, matsutake mushroom, enoki mushroom, the extract of the wood or sawdust and mycelium cultivated situation mushroom or blossom mushroom containing beta glucan component By cultivating the edible mushroom containing beta glucan component by mixing in the culture medium, there is an effect that can be cultivated edible mushrooms exhibiting enhanced immunity and anticancer effect at a low price.

Claims (4)

약용버섯을 원목에 재배하는 경우 그 원목을 톱밥으로 분쇄하거나, 약용버섯을 톱밥 배지에 재배하는 경우, 그 톱밥을 탈병하여 균일한 톱밥을 선별하는 부산물 준비단계;When medicinal mushrooms are cultivated in solid wood, the wood is crushed with sawdust, or when medicinal mushrooms are cultivated in a sawdust medium, by-product preparation step of sorting the uniform sawdust by demineralizing the sawdust; 스테인리스 용기에 당 성분이 함유된 밀기울을 첨가하여 살균한 후, 약용버섯의 종균을 접종하여 일부 배양시켜 액기스화 하는 균사체 액기스 준비단계; 및After sterilizing by adding a bran containing a sugar component to a stainless steel container, inoculated with the seed of the medicinal mushroom incubated part of the mycelium solution preparation step of incubating; And 상기 부산물 준비단계에서 형성된 톱밥과 상기 균사체 준비단계에서 형성된 균사체 액기스와 배지용 톱밥을 혼합하여 배양병에 입병하여 살균기 내에서 살균한 후, 무균실 내에서 냉각시킨 다음, 식용버섯의 종균을 접종하여 배양실에서 배양하며, 배양이 완료된 다음 재배실에서 재배하는 식용버섯 재배단계;를 포함하는 것을 특징으로 하는 식용버섯 재배방법.The sawdust formed in the by-product preparation step and the mycelium extract formed in the mycelium preparation step mixed with the sawdust for medium, and put into a culture bottle, sterilized in a sterilizer, cooled in a sterile chamber, and then inoculated the seed of the edible mushroom in the culture chamber Edible mushroom cultivation method comprising; cultivating in, the edible mushroom cultivation step of cultivation in the cultivation room after the culture is completed. 제 1 항에 있어서,The method of claim 1, 상기 식용버섯 재배단계에서 재배실의 습도를 조절하는 가습수에는 상기 균사체 준비단계에서 형성된 균사체 액기스 3%가 포함된 것을 특징으로 하는 식용버섯 재배방법.Humidifying water to control the humidity of the cultivation room in the edible mushroom cultivation step Edible mushroom cultivation method, characterized in that containing the mycelium extract 3% formed in the mycelium preparation step. 제 1 항에 있어서,The method of claim 1, 상기 식용버섯 재배단계에서의 배양병 입병 비율은, 상기 부산물 준비단계에서 형성된 톱밥 20중량%와, 상기 균사체 준비단계에서 형성된 균사체 액기스 20중량%와 배지용 톱밥 60중량%인 것을 특징으로 하는 식용버섯 재배방법.The cultivation rate of the culture bottle in the edible mushroom cultivation step is 20% by weight of the sawdust formed in the by-product preparation step, 20% by weight of the mycelium solution formed in the mycelium preparation step and 60% by weight of sawdust for medium. Cultivation method. 제 1 항에 있어서,The method of claim 1, 상기 약용버섯은 상황버섯 또는 꽃송이버섯인 것을 특징으로 하는 식용버섯 재배방법.The medicinal mushroom is an edible mushroom cultivation method, characterized in that the situation mushroom or matsutake mushroom.
KR1020060028870A 2006-03-30 2006-03-30 Method growing edible mushroom KR100759021B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020060028870A KR100759021B1 (en) 2006-03-30 2006-03-30 Method growing edible mushroom

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020060028870A KR100759021B1 (en) 2006-03-30 2006-03-30 Method growing edible mushroom

Publications (1)

Publication Number Publication Date
KR100759021B1 true KR100759021B1 (en) 2007-09-17

Family

ID=38737953

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020060028870A KR100759021B1 (en) 2006-03-30 2006-03-30 Method growing edible mushroom

Country Status (1)

Country Link
KR (1) KR100759021B1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101163717B1 (en) 2009-01-07 2012-07-09 한려버섯 영농조합법인 method of growing edible mushrooms
CN104926442A (en) * 2015-06-03 2015-09-23 广西大学 Culture medium for improving quality of black fungus
CN111937679A (en) * 2020-09-01 2020-11-17 郝哲 Phellinus igniarius culture medium and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101163717B1 (en) 2009-01-07 2012-07-09 한려버섯 영농조합법인 method of growing edible mushrooms
CN104926442A (en) * 2015-06-03 2015-09-23 广西大学 Culture medium for improving quality of black fungus
CN111937679A (en) * 2020-09-01 2020-11-17 郝哲 Phellinus igniarius culture medium and preparation method thereof

Similar Documents

Publication Publication Date Title
CN104041330B (en) Ganoderma tsugae imitates wild juggle cultivation method
CN106472104B (en) Manufacturing method for phellinus igniarius cultivation
CN104956914A (en) Breeding method for natural ganoderma lucidum
CN101113415A (en) Method for solid culture of Cordyceps sinensis edible mushroom
CN103918475A (en) Pleurotus geesteranus potted culturing method and culture medium for culturing pleurotus geesteranus
CN101491195A (en) Phlebopus portentosus cultivation method
CN110326485B (en) Cultivation method and application of cultivated trametes heme
KR101330487B1 (en) CULTIVATING METHOD OF MUSHROOM INCLUDING Acanthopanax senticosus HOT WATER EXTRACT
KR20000064891A (en) Artificial fungus cultivation method of fruiting bodies of Agaricus mushroom
CN106069188A (en) A kind of artificial cultivation method of termitomyces
Zhou Cultivation of Ganoderma lucidum
CN112021073A (en) Morchella esculenta external aid nutrition bag ingredient, nutrition bag and preparation method thereof
KR101385540B1 (en) Cultivation method for Cauliflower mushroom
KR101199763B1 (en) A Method of Cultivation OF Sparassis crispa wiht high ßglucan
KR100759021B1 (en) Method growing edible mushroom
JP2000236745A (en) Cultivation of phellinus linteus
CN105296360B (en) Phellinus parent species tissue detaches tube breeding method
KR20100066321A (en) Production method of the cauliflower mushroom hypha using grain medium and the hypha produced thereof
KR100723068B1 (en) Method for culturing flammulina velutipes including ginseng saponin
KR100204984B1 (en) The method of culturing phellinus linteus
KR20120097190A (en) Production method of the clavicoron pyxidata
Mizuno Cultivation of the medicinal mushroom royal sun agaricus—Agaricus blazei Murr.(Agaricomycetideae)
KR101063754B1 (en) Cultivation method for Mycoleptodonoides aitchisonii
KR20160033386A (en) Cultivatio-n method of Fermented phelleniuse linteus using the Cudrania tricuspida-ta and germinated brown rice
KR20190077764A (en) a phellinus linteus cultivating method using Cudrania tricuspidata and the phellinus linteus

Legal Events

Date Code Title Description
A201 Request for examination
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
LAPS Lapse due to unpaid annual fee