CN104920065A - Preparation method for mother strain of wild dictyophora rubrovolvatain of Mount Fanjing - Google Patents
Preparation method for mother strain of wild dictyophora rubrovolvatain of Mount Fanjing Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention discloses a preparation method for a mother strain of wild dictyophora rubrovolvatain of Mount Fanjing. The preparation method includes taking talinum paniculatum, rice, glucose and agar as raw materials, and preparing a talinum paniculatum test tube bevel culture medium serving as a mother strain culture medium for standby application; collecting strain eggs of the wild dictyophora rubrovolvatain of the Mount Fanjing, wrapping and sealing each strain egg with a preservative film immediately, and taking the strain eggs to a laboratory; disinfecting the strain eggs with tap water, alcohol, sodium hypochlorite, sterile water and the like, cutting the strain eggs open, taking out strain stems, cutting the strain stems into a plurality of blocks with the side length of 0.5-1cm, and inoculating each mother strain culture medium test tube with one block, wherein each strain egg can be used for inoculation of 8-12 test tubes; culturing the inoculated test tubes at the temperature of 20 DEG C in a dark place for 45 days to obtain the mother strain of the wild dictyophora rubrovolvatain of the Mount Fanjing. The preparation method has the advantages that the talinum paniculatum substitutes for chemical reagents added into ordinary culture media, simplicity and convenience in operation, low cost and short cycle are achieved, chemical pollution is avoided, and mother strains of similar edible mushrooms such as dictyophora indusiata can be prepared.
Description
Technical field
The present invention relates to the Breeding of Edible Mushroom and production field, specifically the wild Dictyophora rubrovalvata mother of a kind of Mount Fanjing plants preparation method.
Background technology
Talinum crassifolium (
talinum paniculatum(Jacq.) Gaertn.) have another name called native American ginseng, panicled fameflower root, ginseng dish etc., be the perennial herbaceous plant of Portulacaceae.In the leaf of Talinum crassifolium, oxalic acid content is higher, is rich in the compositions such as calcium, phosphorus, iron, ascorbic acid, thiamine; The similar ginseng of its root, containing glucide, can stew or steep in wine; Tender leaf, tender tip edible, also can cold and dressed with sauce, stir-fry and eat, taste is fresh and tender.And the Chinese herbal medicine that Talinum crassifolium is still fabulous simply, has effect of invigorating the spleen moistening lung, cough-relieving, menstruation regulating, be described as " southern ginseng ", gourmet thinks " Talinum crassifolium is mended and surpassed ginseng ", but its exploitation research is less.Therefore; this research team is the multiple use of exploitation Talinum crassifolium; carry out positive exploration; obtain a patent for an invention " a kind of Talinum crassifolium list leaf cuttage breeding method ", captured Talinum crassifolium biological control technology, but research finds; in vitro Talinum crassifolium root, stem and leaf all easily grows white fungi; so the imagination of the novelty teabag (cultivating the edible mushroom of high value) of exploitation Talinum crassifolium has put forward, namely realize " production of medicinal plant Talinum crassifolium---dictyophora phalloidea (
dictyophpraspp) produce---Talinum crassifolium produce " " medicinal plant and edible mushroom " optimum free of contamination circulation production model.Inspection information finds, this Talinum crassifolium carries out the research of dictyophora phalloidea production as one of primary raw material, or the field that brand-new.
Dictyophora phalloidea (
dictyophpraspp) have another name called bamboo bacterium, bamboo ginseng, celestial being bacterium etc., be subordinate to the macro fungi that Eumycota Basidiomycotina Gasteromycete Phallales Phallaceae dictyophora phalloidea belongs to.The tame edible dictyophora phalloidea of China have short-skirted veiled lady (
phallus duplicatusbosc Fisch), Cultivation of Dictyophora (
phallus indusiatusvent.), Dictyophora rubrovalvata (
dictyophora rubrovoluatazang, Ji et Liou) and thorn holder dictyophora phalloidea (
dictyophora echinouloluatazang, Zheng.et Hu) 4 kinds, wherein best with Dictyophora rubrovalvata variety and quality, selling price is the highest, about 700 Yuan/㎏.Dictyophora phalloidea produces, and the output value is about ten thousand yuan of/mu of years of 3-6, is take in one of the highest edible mushroom kind.Dictyophora phalloidea contains the trace element of 19 seed amino acids, multivitamin and useful health, a kind of full nutrition, natural food, rank first of " four is precious " i.e. dictyophora phalloidea, hedgehog hydnum, mushroom, white fungus, deeply welcome by consumers in general, reputation is high in the international market, once there is saying of " dictyophora phalloidea gold price ", received the concern of many scholars.At present, organic matter extracting and developing, qualification are mainly concentrated on to the research of dictyophora phalloidea, extract chemical composition and bacteriostasis, the several functions activity analysiss such as anti-oxidant, reducing blood lipid, raising immunity, the extraction of polysaccharide, glycoprotein, agglutinin, purifying, the aspect such as qualification and biochemical characteristic, but at wild varieties domestication, dictyophora phalloidea breeding of new varieties, (female kind is the one in edible fungus species to dictyophora phalloidea mother kind, is the most critical part of strain quality.Bacterial classification refers to that the fruit body (edible part) of edible mushroom or spore (being equivalent to the seed of plant) cultivate through artificial the pure mycelium formed.Aborning according to the source of bacterial classification, reproductive order of generation and production object, be divided into female kind, original seed and cultivated species bacterial classification, be one-level kind, secondary kind and three grades of kinds respectively.Female kind refers to a part or the spore of application fruit body of edible fungi, the pure mycelium of breeding in the medium.Female kind can breed original seed, also can be used for culture presevation.Original seed refers to female secondary mycelia planted mycelia and breed on solid culture medium, i.e. secondary kind, and this process strengthens mycelia to the adaptability of culture environment, plays again and expands numerous effect.Original seed can direct fruiting.Cultivated species refers to and is formed by protospecies breeding, mainly numerous in order to expand, in order to produce, enough bacterial classifications are provided) research aspect such as cultivation grade, there is dictyophora phalloidea of less types, use chemical reagent, need fresh bamboo, wastage of material is (in potato culture making, potato only gets its soup juice, major part potato residues body is dropped), manufacture craft is relatively loaded down with trivial details (in medium making, need to filter or adjust ph etc.) etc. problem, this is also the immediate cause of dictyophora phalloidea price high (about 700 Yuan/㎏), therefore, probe into wild varieties domestication, dictyophora phalloidea breeding of new varieties, apply free of contamination raw material, few with or without chemical reagent, reduce production technology link, reduce costs, have important practical significance in dictyophora phalloidea produces.
In addition, the wild Dictyophora rubrovalvata beautiful appearance of Mount Fanjing, product are on very good terms, individual large, have great productive potential, but still be in wild state, untapped utilization, and to develop one of key technology of the wild Dictyophora rubrovalvata of Mount Fanjing be make female kind, Mount Fanjing wild Dictyophora rubrovalvata bacterium egg (a kind of fruiting state when bacterium egg refers to that dictyophora phalloidea forms fruit body (edible part), also be button, shape is as egg.When button top projection is as peach-shaped, how to be ftractureed by bossing in the morning of fine day, firstly appear out from cap, stem extends in succession, stop extending when stem at noon grows to certain altitude, indusium by drops down in lid, forms the dictyophora phalloidea that can pluck gradually) be make one of female best explant (as the fragment of the organ or tissue of cultured in vitro material in Plant Tissue Breeding) of planting.
Summary of the invention
This patent aims to provide a kind of Mount Fanjing wild dictyophora phalloidea mother and plants preparation method, instead of with Talinum crassifolium the chemical reagent that general medium adds, easy and simple to handle, cost is low, and the cycle is short, without chemical contamination, and can make mother's kind of the similar edible mushrooms such as Cultivation of Dictyophora.
The present invention is achieved in that its operating procedure is:
(1) the preparation of mother culture media
Take dry Talinum crassifolium root, totally 3 ~ 6 grams, stem or leaf, 50 grams, rice, (standard of degree of grinding is when heating preparation medium to use cracker co-grinding, Talinum crassifolium and rice mixed-powder can form the mixed liquor that suspends uniformly), obtain Talinum crassifolium and rice mixed-powder, add in 800 ml distilled waters, add 20 grams, agar again, glucose 20 grams, boil to agar with micro-wave oven and dissolve completely, be settled to 1000 milliliters, be dispensed into test tube again, often prop up the mixing material of test tube packing 1/4 ~ 1/3 test tube height, cover cap test tube, often prop up cap test tube one end of test tube, wrap 2 layers of paper, encase cap test tube completely, bind with rope, prevent the test tube of cap test tube one end from directly contacting with air, 121 DEG C of high pressure steam sterilizations, make Talinum crassifolium test tube slant medium, as mother culture media (large-scale mother culture media for subsequent use, can prepare according to the above ratio, make Talinum crassifolium test tube slant medium for subsequent use).
(2) Mount Fanjing wild Dictyophora rubrovalvata bacterium egg process
Gather Mount Fanjing wild Dictyophora rubrovalvata bacterium egg, remove the impurity outside bacterium egg, preservative film environmental sealing used immediately by each bacterium egg, takes back laboratory for subsequent use; Bacterium egg tap water 1 ~ 2 hour, then after spraying with 75% alcohol, on superclean bench, with 75% alcohol-pickled 90 seconds, then uses 2% hypochlorite disinfectant 30 minutes, finally uses sterile water wash 8 times, obtain the bacterium egg after sterilizing, for subsequent use.
(3) inoculate
Cut the bacterium egg after sterilization, take out stem, stem is cut into the square of several 0.5 ~ 1 centimeter sides, often prop up test tube and inoculate 1 piece of square, inoculate 1 piece of square and be placed in test tube bottom end, on the Talinum crassifolium slant medium of about 1/3rd test tube length, each bacterium egg can inoculate 8 ~ 12 test tubes, cover cap test tube, often prop up cap test tube one end of test tube, wrap 2 layers of paper, encase cap test tube completely, bind with rope, prevent the test tube of cap test tube one end from directly contacting with air, the test tube that must have inoculated.
(4) constant temperature culture
The test tube that above-mentioned inoculation is good, the constant incubator lucifuge being placed in 20 DEG C is cultivated, and within about 45 days, namely obtains and can obtain Mount Fanjing wild Dictyophora rubrovalvata mother kind.At this moment, female kind can as culture presevation, also can transfer 1 time, the mother often propping up test tube (diameter 20mm × long 200mm) plants the original seed can transferring into 12 ~ 18 test tubes, when mycelia is paved with test tube 2/3rds, transfer again 1 time, often prop up the original seed of test tube (diameter 20mm × long 200mm), the numerous original seed of expansion of 12 ~ 18 test tubes can be transferred into equally, start the clock from the inoculation of bacterium egg, about 4 months, 1 bacterium egg can obtain the original seed of the wild Dictyophora rubrovalvata original seed of Mount Fanjing 1152 ~ 3888 test tubes.
Said method also can make mother's kind of Cultivation of Dictyophora etc. and the similar edible mushroom of the wild Dictyophora rubrovalvata of Mount Fanjing.
The present invention's Talinum crassifolium instead of general medium add chemical reagent, for realize " production of medicinal plant Talinum crassifolium---dictyophora phalloidea (
dictyophpraspp) produce---Talinum crassifolium produce " " medicinal plant and edible mushroom " optimum free of contamination circulation production model provide key technology, can be used for the aspects such as the domestication of the similar edible mushroom of dictyophora phalloidea wild with Mount Fanjing, breeding and large-scale production.Compared with prior art, the present invention has following advantage:
1, the data display of development test and documents and materials, the present invention has turned out Mount Fanjing wild Dictyophora rubrovalvata mother first time and has planted.
2, Talinum crassifolium instead of the chemical reagent that general medium adds, and has the feature without chemical contamination, and cultivates the female kind speed of the wild Dictyophora rubrovalvata of Mount Fanjing soon, about 45 days, as long as the shortest 30 days.
3, compare with fresh bamboo medium with common potato culture, eliminate the manufacture craft link of filtration, decrease the waste (residue of potato and fresh bamboo all goes out of use) of raw material, and the mycelial growth of Talinum crassifolium medium culture is faster, female kind is with short production cycle.
4, compared with general medium, purposes is more extensive, can make mother's kind of Cultivation of Dictyophora etc. and the similar edible mushroom of the wild Dictyophora rubrovalvata of Mount Fanjing.
5, reproduction coefficient of the present invention is large, the wild Dictyophora rubrovalvata original seed of Mount Fanjing that a bacterium egg can be trained 1152 ~ 3888 test tubes.
6, this invention exploits the novelty teabag of Talinum crassifolium: being that one of primary raw material making dictyophora phalloidea is female with Talinum crassifolium plants, and is conducive to " medicinal plant and edible mushroom " the optimum free of contamination circulation production model realizing " production of the production of medicinal plant Talinum crassifolium---dictyophora phalloidea production---Talinum crassifolium ".
7, the technology of the present invention can be used for domestication, the breeding of the wild fungis such as the wild Dictyophora rubrovalvata of Mount Fanjing, is convenient to the research of similar fungal physiology function, hybrid vigour and genetic development.
Embodiment
The embodiment of the present invention
Take dry Talinum crassifolium root, totally 3 ~ 6 grams, stem or leaf, 50 grams, rice, (standard of degree of grinding is when heating preparation medium to use cracker co-grinding, Talinum crassifolium and rice mixed-powder can form the mixed liquor that suspends uniformly), obtain Talinum crassifolium and rice mixed-powder, add in 800 ml distilled waters, add 20 grams, agar again, glucose 20 grams, boil to agar with micro-wave oven and dissolve completely, be settled to 1000 milliliters, be dispensed into test tube again, often prop up the mixing material of test tube packing 1/4 ~ 1/3 test tube height, cover cap test tube, often prop up cap test tube one end of test tube, wrap 2 layers of paper, encase cap test tube completely, bind with rope, prevent the test tube of cap test tube one end from directly contacting with air, 121 DEG C of high pressure steam sterilizations, make Talinum crassifolium test tube slant medium, for subsequent use as mother culture media.Gather Mount Fanjing wild Dictyophora rubrovalvata bacterium egg, remove the impurity outside bacterium egg, preservative film environmental sealing used immediately by each bacterium egg, takes back laboratory for subsequent use; Bacterium egg tap water 1 ~ 2 hour, then after spraying with 75% alcohol, on superclean bench, with 75% alcohol-pickled 90 seconds, then uses 2% hypochlorite disinfectant 30 minutes, finally uses sterile water wash 8 times, obtain the bacterium egg after sterilizing, for subsequent use.Cut the bacterium egg after sterilization, take out stem, stem is cut into the square of several 0.5 ~ 1 centimeter sides, often prop up test tube and inoculate 1 piece of square, inoculate 1 piece of square and be placed in test tube bottom end, on the Talinum crassifolium slant medium of about 1/3rd test tube length, each bacterium egg can inoculate 8 ~ 12 test tubes, cover cap test tube, often prop up cap test tube one end of test tube, wrap 2 layers of paper, encase cap test tube completely, bind with rope, prevent the test tube of cap test tube one end from directly contacting with air, the test tube that must have inoculated.The test tube that above-mentioned inoculation is good, the constant incubator lucifuge being placed in 20 DEG C is cultivated, and within about 45 days, namely obtains and can obtain Mount Fanjing wild Dictyophora rubrovalvata mother kind.
Claims (1)
1. the wild Dictyophora rubrovalvata mother of Mount Fanjing plants a preparation method, it is characterized in that: operating procedure is as follows:
(1) the preparation of mother culture media
Take dry Talinum crassifolium root, totally 3 ~ 6 grams, stem or leaf, 50 grams, rice, (standard of degree of grinding is when heating preparation medium to use cracker co-grinding, Talinum crassifolium and rice mixed-powder can form the mixed liquor that suspends uniformly), obtain Talinum crassifolium and rice mixed-powder, add in 800 ml distilled waters, add 20 grams, agar again, glucose 20 grams, boil to agar with micro-wave oven and dissolve completely, be settled to 1000 milliliters, be dispensed into test tube again, often prop up the mixing material of test tube packing 1/4 ~ 1/3 test tube height, cover cap test tube, often prop up cap test tube one end of test tube, wrap 2 layers of paper, encase cap test tube completely, bind with rope, prevent the test tube of cap test tube one end from directly contacting with air, 121 DEG C of high pressure steam sterilizations, make Talinum crassifolium test tube slant medium, as mother culture media (large-scale mother culture media for subsequent use, can prepare according to the above ratio, make Talinum crassifolium test tube slant medium for subsequent use),
(2) Mount Fanjing wild Dictyophora rubrovalvata bacterium egg process
Gather Mount Fanjing wild Dictyophora rubrovalvata bacterium egg, remove the impurity outside bacterium egg, preservative film environmental sealing used immediately by each bacterium egg, takes back laboratory for subsequent use; Bacterium egg tap water 1 ~ 2 hour, then after spraying with 75% alcohol, on superclean bench, with 75% alcohol-pickled 90 seconds, then uses 2% hypochlorite disinfectant 30 minutes, finally uses sterile water wash 8 times, obtain the bacterium egg after sterilizing, for subsequent use;
(3) inoculate
Cut the bacterium egg after sterilization, take out stem, stem is cut into the square of several 0.5 ~ 1 centimeter sides, often prop up test tube and inoculate 1 piece of square, inoculate 1 piece of square and be placed in test tube bottom end, on the Talinum crassifolium slant medium of about 1/3rd test tube length, each bacterium egg can inoculate 8 ~ 12 test tubes, cover cap test tube, often prop up cap test tube one end of test tube, wrap 2 layers of paper, encase cap test tube completely, bind with rope, prevent the test tube of cap test tube one end from directly contacting with air, the test tube that must have inoculated;
(4) constant temperature culture
The test tube that above-mentioned inoculation is good, the constant incubator lucifuge being placed in 20 DEG C is cultivated, and within about 45 days, namely obtains and can obtain Mount Fanjing wild Dictyophora rubrovalvata mother kind; At this moment, female kind can as culture presevation, also can transfer 1 time, the mother often propping up test tube (diameter 20mm × long 200mm) plants the original seed can transferring into 12 ~ 18 test tubes, when mycelia is paved with test tube 2/3rds, transfer again 1 time, often prop up the original seed of test tube (diameter 20mm × long 200mm), the numerous original seed of expansion of 12 ~ 18 test tubes can be transferred into equally, start the clock from the inoculation of bacterium egg, about 4 months, 1 bacterium egg can obtain the original seed of the wild Dictyophora rubrovalvata original seed of Mount Fanjing 1152 ~ 3888 test tubes.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754394A (en) * | 2016-07-10 | 2017-05-31 | 贵州省农作物品种资源研究所 | It is suitable for the Dictyophora rubrovalvata yzs020 bacterial strains of substituting stuff cultivation |
| CN110637681A (en) * | 2018-06-27 | 2020-01-03 | 贵州金蟾大山生物科技有限责任公司 | Method for culturing Dictyophora rubrovalvata mother strain |
| CN111084054A (en) * | 2020-02-21 | 2020-05-01 | 铜仁学院 | Method for preparing hericium erinaceus strain from Huangjiang flower fungus material |
| CN111194663A (en) * | 2019-03-26 | 2020-05-26 | 贵州久源顺生态农业开发有限责任公司 | Dictyophora thermosphakii cultivation method |
| CN111903432A (en) * | 2020-08-06 | 2020-11-10 | 贵州珍稀食用菌科技有限公司 | Dictyophora rubrovalvata strain rapid propagation method and application thereof |
| CN112586273A (en) * | 2020-12-17 | 2021-04-02 | 贵州丰源现代农业有限公司 | Culture medium, preparation method thereof, Dictyophora rubrovalvata mother strain culture method and application |
| CN112931058A (en) * | 2021-01-29 | 2021-06-11 | 杭州市农业科学研究院 | Separation and purification method of Moganshan wild dictyophora rubrovolvata strain |
| CN115404171A (en) * | 2022-08-31 | 2022-11-29 | 贵州金荪生物科技开发有限责任公司 | Preparation method of Dictyophora rubrovalvata liquid mother strain |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111084054A (en) * | 2020-02-21 | 2020-05-01 | 铜仁学院 | Method for preparing hericium erinaceus strain from Huangjiang flower fungus material |
| CN111903432A (en) * | 2020-08-06 | 2020-11-10 | 贵州珍稀食用菌科技有限公司 | Dictyophora rubrovalvata strain rapid propagation method and application thereof |
| CN112586273A (en) * | 2020-12-17 | 2021-04-02 | 贵州丰源现代农业有限公司 | Culture medium, preparation method thereof, Dictyophora rubrovalvata mother strain culture method and application |
| CN112931058A (en) * | 2021-01-29 | 2021-06-11 | 杭州市农业科学研究院 | Separation and purification method of Moganshan wild dictyophora rubrovolvata strain |
| CN115404171A (en) * | 2022-08-31 | 2022-11-29 | 贵州金荪生物科技开发有限责任公司 | Preparation method of Dictyophora rubrovalvata liquid mother strain |
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