CN104920065B - A kind of wild Dictyophora rubrovalvata parent species preparation method of Mount Fanjing - Google Patents
A kind of wild Dictyophora rubrovalvata parent species preparation method of Mount Fanjing Download PDFInfo
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- CN104920065B CN104920065B CN201510268986.5A CN201510268986A CN104920065B CN 104920065 B CN104920065 B CN 104920065B CN 201510268986 A CN201510268986 A CN 201510268986A CN 104920065 B CN104920065 B CN 104920065B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a kind of wild Dictyophora rubrovalvata parent species preparation method of Mount Fanjing, comprises the steps：By a certain percentage, with Talinum crassifolium, rice, glucose and agar as raw material, Talinum crassifolium test tube slant culture medium is made, standby as mother culture media；The bacterium egg of the wild Dictyophora rubrovalvata of collection Mount Fanjing, each bacterium egg are used preservative film environmental sealing immediately, take back laboratory；After the sterilization such as bacterium egg running water, alcohol, sodium hypochlorite and sterilized water, bacterium egg is cut, stem is taken out, stem is cut into the square of several 0.5～1 centimeter sides, every mother culture media test tube is inoculated with 1 square, and each bacterium egg can be inoculated with 8～12 test tubes；The test tube being inoculated with, 20 DEG C of lucifuge cultures, 45 days or so, you can obtain the wild Dictyophora rubrovalvata parent species of Mount Fanjing.Present invention Talinum crassifolium instead of general culture medium interpolation chemical reagent, easy to operate, low cost, cycle is short, nothing chemical contamination, and the parent species of the similar edible mushroom such as Cultivation of Dictyophora can be made.
Description
Technical field
The present invention relates to the Breeding of Edible Mushroom and production field, the wild Dictyophora rubrovalvata parent species making side of specifically a kind of Mount Fanjing
Method.
Background technology
Talinum crassifolium（Talinum paniculatum(Jacq.) Gaertn.）Also known as native American ginseng, panicled fameflower root, ginseng dish
Deng being the perennial herbaceous plant of Portulacaceae.In the leaf of Talinum crassifolium, oxalic acid content is higher, rich in calcium, phosphorus, iron, Vitamin C
The compositions such as acid, thiamine；Its root is similar to ginseng, containing glucide, can stew or steep in wine；Tender leaf, tender tip edible, also may be used
Cold and dressed with sauce, stir-fry and eat, taste is fresh and tender.And, the still fabulous simply Chinese herbal medicine of Talinum crassifolium, with invigorating the spleen moistening lung, cough-relieving, menstruation regulating work(
Effect, is described as " south ginseng ", and gourmet thinks " Talinum crassifolium is mended and surpasses ginseng ", but its exploitation research is less.Therefore, originally
Research team is the multiple use for developing Talinum crassifolium, has carried out positive exploration, has obtained a patent for an invention《A kind of Talinum crassifolium list
Leaf cuttage breeding method》, Talinum crassifolium biological control technology to be captured, but research has found, in vitro Talinum crassifolium root, stem and leaf all holds
Easily the white fungi of growth, then, develops the new application of Talinum crassifolium（The edible mushroom of culture high value）Imagination put forward, also
Be realize " the production dictyophora phalloidea of medicinal plant Talinum crassifolium (Dictyophpraspp) production Talinum crassifolium production " " medicinal
The optimum free of contamination circulation production model of plant and edible mushroom ".Inspection information find, this use Talinum crassifolium as primary raw material it
One research for carrying out dictyophora phalloidea production, or a brand-new field.
Dictyophora phalloidea (Dictyophpraspp) also known as bamboo bacterium, bamboo ginseng, celestial being bacterium etc., it is subordinate to Eumycota Basidiomycotina Gasteromycete
The macro fungi of Phallales Phallaceae dictyophora phalloidea category.The tame edible dictyophora phalloidea of China has short-skirted veiled lady（Phallus duplicatusBosc Fisch）, Cultivation of Dictyophora（Phallus indusiatusVent.）, Dictyophora rubrovalvata
（Dictyophora rubrovoluataZang,Ji et Liou）With thorn support dictyophora phalloidea（Dictyophora echinouloluataZang,Zheng.et Hu）4 kinds, wherein with Dictyophora rubrovalvata variety and quality preferably, selling price
Highest, about 700 yuan/.Dictyophora phalloidea produces, 10000 yuan/mu year of the output value about 3-6, is one of income highest edible mushroom species.Dictyophora phalloidea
Trace element containing 19 kinds of amino acid, multivitamin and beneficial health, is a kind of full nutrition, natural food, name
First of row " four is precious " i.e. dictyophora phalloidea, hedgehog hydnum, mushroom, white fungus, welcome by consumers in general deeply, reputation is high in the international market, once
There is saying for " dictyophora phalloidea gold price ", paid close attention to by many scholars.At present, the research of dictyophora phalloidea is concentrated mainly on organic matter extract,
Separate, identify, extract chemical constituent and bacteriostasis, the several functions such as anti-oxidant, reducing blood lipid, enhance immunity activity point
Analysis, the aspect such as polysaccharide, glycoprotein, the extraction of agglutinin, purifying, identification and biochemical characteristic, but new in wild varieties domestication, dictyophora phalloidea
Breeding for quality, dictyophora phalloidea parent species（Parent species are the one kind in edible fungus species, are the most critical parts of strain quality.Bacterial classification refers to food
Fructification with bacterium（Edible part）Or the pure mycelium that spore (equivalent to seed of plant) is formed through artificial culture.Giving birth to
According to the source of bacterial classification in product, reproductive order of generation and production purpose, bacterial classification is divided into parent species, original seed and cultigen, is one-level respectively
Kind, two grades of kinds and three-level kind.Parent species refer to apply a part or the spore of fruit body of edible fungi, breed in the medium
Pure mycelium.Parent species can breed original seed, it can also be used to culture presevation.Original seed refers to that parent species mycelia is bred on solid medium
Two grades of mycelia, i.e., two grades kinds, this process strengthen the adaptability of mycelia to culture environment, play again and expand numerous effect.
Original seed can direct fruiting.Cultigen refers to be formed by protospecies breeding, primarily to expansion is numerous, in order to produce, provides enough
Bacterial classification）Culture etc. is in terms of research, exist dictyophora phalloidea of less types, using chemical reagent, need fresh bamboo, wastage of material（Ma Ling
In potato culture matrix manufacturing, potato only takes its soup, and most of potato residues body is dropped）, manufacture craft relatively cumbersome（Training
In foster matrix manufacturing, need to filter or adjust pH value etc.）The problems such as, this is also that dictyophora phalloidea price is high（About 700 yuan/）Direct original
Cause, therefore, probe into wild varieties domestication, dictyophora phalloidea breeding of new varieties, apply free of contamination raw material, few with or without chemical reagent,
Reduce production technology link, reduces cost etc., have important practical significance in dictyophora phalloidea production.
In addition, the wild Dictyophora rubrovalvata beautiful appearance of Mount Fanjing, condition is good, individual big, with great productive potentialities, but still
In wild state, untapped utilization, and it is to make parent species to develop one of key technology of the wild Dictyophora rubrovalvata of Mount Fanjing,
The wild Dictyophora rubrovalvata bacterium egg of Mount Fanjing（Bacterium egg refers to that dictyophora phalloidea forms fructification（Edible part）When a kind of fruiting state, also cry
Button, shape such as egg.When the raised such as peach-shaped in button top, how to be ftractureed by bossing in the morning of fine day, cap firstly appeared out from,
Stem extends in succession, stops elongation when growing to certain altitude to noon stem, and, gradually by drops down in lid, formation can for indusium
With the dictyophora phalloidea that plucks）It is the best explant for making parent species（In Plant Tissue Breeding as cultured in vitro material organ or group
The fragment that knits）One of.
Content of the invention
This patent aims to provide a kind of wild dictyophora phalloidea parent species preparation method of Mount Fanjing, instead of general culture medium with Talinum crassifolium
The chemical reagent of interpolation, easy to operate, low cost, cycle is short, nothing chemical contamination, and the similar food such as Cultivation of Dictyophora can be made
Parent species with bacterium.
The present invention is realized in：Its operating procedure is：
(1) the preparation of mother culture media
Totally 3～6 grams of dry Talinum crassifolium root, stem or leaf is weighed, 50 grams of rice, using pulverizer co-grinding（Crush journey
The standard of degree is that Talinum crassifolium and rice mixed-powder can form uniform suspension mixed solution when culture medium is prepared in heating）, obtain
Talinum crassifolium and rice mixed-powder, add in 800 milliliters of distilled water, add 20 grams of agar, 20 grams of glucose, boiled with micro-wave oven
Boil and be completely dissolved to agar, 1000 milliliters are settled to, then test tube is dispensed into, every test tube dispenses the mixed of 1/4～1/3 test tube height
Liquid is closed, covers cap test tube, cap test tube one end of every test tube, 2 layers of paper are wrapped, encases cap test tube completely, bound with rope, prevent
The test tube of cap test tube one end directly and air contact, 121 DEG C of high pressure steam sterilizations, makes Talinum crassifolium test tube slant culture medium, makees
Standby for mother culture media（Large-scale mother culture media, can be prepared according to the above ratio, be made Talinum crassifolium test tube slant
Culture medium is standby）.
(2) the wild Dictyophora rubrovalvata bacterium egg of Mount Fanjing is processed
The wild Dictyophora rubrovalvata bacterium egg of collection Mount Fanjing, removes the impurity outside bacterium egg, and each bacterium egg is wrapped up with preservative film immediately
Sealing, takes back laboratory standby；Bacterium egg is rinsed 1～2 hour with running water, then with after the sprinkling of 75% alcohol, in superclean bench
On, with 75% alcohol-pickled 90 seconds, then with 2% hypochlorite disinfectant 30 minutes, finally with sterile water wash 8 times, after obtaining sterilization
Bacterium egg, standby.
(3) it is inoculated with
The bacterium egg after sterilization is cut, stem is taken out, stem is cut into the square of several 0.5～1 centimeter sides, per an examination
Pipe is inoculated with 1 piece of square, and 1 piece of square of inoculation is placed in test tube bottom end, the Talinum crassifolium inclined-plane culture of about 1/3rd test tube length
On base, each bacterium egg can be inoculated with 8～12 test tubes, cover cap test tube, cap test tube one end of every test tube, wrap 2 layers of paper, wrap completely
Firmly cap test tube, is bound with rope, is prevented the test tube of cap test tube one end directly and air contact, is obtained final product the test tube being inoculated with.
(4) incubated
The good test tube of above-mentioned inoculation, is placed in 20 DEG C of constant incubator lucifuge culture, obtains final product i.e. within 45 days or so and Buddhist is obtained
Net hill life Dictyophora rubrovalvata parent species.At this moment, parent species can be used as culture presevation, it is also possible to transfer 1 time, every test tube（Diameter
20mm × long 200mm）Parent species can transfer into the original seed of 12～18 test tubes, when mycelia is paved with test tube 2/3rds, then
Switching 1 time, every test tube（Diameter 20mm × long 200mm）Original seed, can equally transfer into the numerous original of expansion of 12～18 test tubes
Kind, start the clock from the inoculation of bacterium egg, about 4 months, 1 bacterium egg be obtained the wild Dictyophora rubrovalvata original seed of Mount Fanjing 1152～
The original seed of 3888 test tubes.
Said method can also make the parent species of the edible mushroom similar to the wild Dictyophora rubrovalvata of Mount Fanjing such as Cultivation of Dictyophora.
Present invention Talinum crassifolium instead of the chemical reagent of general culture medium interpolation, be to realize " medicinal plant Talinum crassifolium
Production dictyophora phalloidea (Dictyophpraspp) production Talinum crassifolium production " " medicinal plant and edible mushroom " optimum pollution-free
Circulation production model provide key technology, can be used for the domestication of the edible mushroom similar with the wild dictyophora phalloidea of Mount Fanjing, breeding and
The aspects such as large-scale production.Compared with prior art, the present invention has following advantage：
1st, the data display of development test and documents and materials, the present invention have turned out the wild Dictyophora rubrovalvata of Mount Fanjing for the first time
Parent species.
2nd, Talinum crassifolium instead of the chemical reagent of general culture medium interpolation, have the characteristics that nothing chemical contamination, and cultivate of ancient India
Net hill life Dictyophora rubrovalvata parent species speed is fast, about 45 days, as long as most short 30 days.
3rd, compared with common potato culture and fresh bamboo culture medium, the manufacture craft link of filtration is eliminated,
Reduce the waste of raw material（The residue of potato and fresh bamboo all goes out of use）, and the mycelia life of Talinum crassifolium medium culture
Faster, parent species are with short production cycle for length.
4th, compared with general culture medium, purposes is more extensive, can make Cultivation of Dictyophora etc. with the wild Dictyophora rubrovalvata of Mount Fanjing
The parent species of similar edible mushroom.
5th, breeding coefficient of the present invention is big, and a bacterium egg can be trained the Mount Fanjing wild red support bamboo of 1152～3888 test tubes
Sweet-smelling grass original seed.
6th, the present invention develops the new application of Talinum crassifolium：It is that one of primary raw material makes dictyophora phalloidea parent species with Talinum crassifolium, favorably
Good in " medicinal plant and the edible mushroom " of realization " the production dictyophora phalloidea production Talinum crassifolium production of medicinal plant Talinum crassifolium "
The free of contamination circulation production model of property.
7th, the technology of the present invention can be used for the domestication of wild fungi, the breedings such as the wild Dictyophora rubrovalvata of Mount Fanjing, be easy to class likelihood
The research of bacterium physiological function, hybrid vigour and genetic development.
Specific embodiment
The embodiment of the present invention
Totally 3～6 grams of dry Talinum crassifolium root, stem or leaf is weighed, 50 grams of rice, using pulverizer co-grinding（Crush journey
The standard of degree is that Talinum crassifolium and rice mixed-powder can form uniform suspension mixed solution when culture medium is prepared in heating）, obtain
Talinum crassifolium and rice mixed-powder, add in 800 milliliters of distilled water, add 20 grams of agar, 20 grams of glucose, boiled with micro-wave oven
Boil and be completely dissolved to agar, 1000 milliliters are settled to, then test tube is dispensed into, every test tube dispenses the mixed of 1/4～1/3 test tube height
Liquid is closed, covers cap test tube, cap test tube one end of every test tube, 2 layers of paper are wrapped, encases cap test tube completely, bound with rope, prevent
The test tube of cap test tube one end directly and air contact, 121 DEG C of high pressure steam sterilizations, makes Talinum crassifolium test tube slant culture medium, makees
Standby for mother culture media.The wild Dictyophora rubrovalvata bacterium egg of collection Mount Fanjing, removes the impurity outside bacterium egg, and each bacterium egg is immediately with guarantor
Fresh film environmental sealing, takes back laboratory standby；Bacterium egg is rinsed 1～2 hour with running water, then with after the sprinkling of 75% alcohol, ultra-clean
On workbench, with 75% alcohol-pickled 90 seconds, then with 2% hypochlorite disinfectant 30 minutes, finally with sterile water wash 8 times, obtain
Bacterium egg after sterilization, standby.The bacterium egg after sterilization is cut, stem is taken out, stem is cut into several 0.5～1 centimeter sides
Square, every test tube are inoculated with 1 piece of square, and 1 piece of square of inoculation is placed in test tube bottom end, the natives of about 1/3rd test tube length
On ginseng slant medium, each bacterium egg can be inoculated with 8～12 test tubes, cover cap test tube, cap test tube one end of every test tube, bag 2
Layer paper, encases cap test tube completely, is bound with rope, prevents the test tube of cap test tube one end directly and air contact, obtains final product and be inoculated with
Test tube.The good test tube of above-mentioned inoculation, is placed in 20 DEG C of constant incubator lucifuge culture, obtains final product i.e. within 45 days or so and Buddhist is obtained only
Hill gives birth to Dictyophora rubrovalvata parent species.
Claims (1)
1. the wild Dictyophora rubrovalvata parent species preparation method of a kind of Mount Fanjing, it is characterised in that：Operating procedure is as follows：
(1) the preparation of mother culture media
Weigh totally 3～6 grams of dry Talinum crassifolium root, stem or leaf, 50 grams of rice, using pulverizer co-grinding, degree of grinding
Standard is that Talinum crassifolium and rice mixed-powder can form uniform suspension mixed solution, obtain natives when culture medium is prepared in heating
Ginseng and rice mixed-powder, add 800 milliliters of distilled water in, add 20 grams of agar, 20 grams of glucose, with micro-wave oven boil to
Agar is completely dissolved, and is settled to 1000 milliliters, then is dispensed into test tube, and every test tube dispenses the mixed liquor of 1/4～1/3 test tube height
Body, covers cap test tube, cap test tube one end of every test tube, wraps 2 layers of paper, encases cap test tube completely, is bound with rope, prevents test tube
The test tube of cap one end directly and air contact, 121 DEG C of high pressure steam sterilizations, makes Talinum crassifolium test tube slant culture medium, as mother
Plant culture medium standby, large-scale mother culture media, can be prepared according to the above ratio, be made the culture of Talinum crassifolium test tube slant
Base is standby；
(2) the wild Dictyophora rubrovalvata bacterium egg of Mount Fanjing is processed
The wild Dictyophora rubrovalvata bacterium egg of collection Mount Fanjing, removes the impurity outside bacterium egg, and preservative film environmental sealing used immediately by each bacterium egg,
Take back laboratory standby；Bacterium egg is rinsed 1～2 hour with running water, then with after the sprinkling of 75% alcohol, on superclean bench, is used
75% alcohol-pickled 90 seconds, then with 2% hypochlorite disinfectant 30 minutes, finally with sterile water wash 8 times, obtain the bacterium after sterilization
Egg, standby；
(3) it is inoculated with
The bacterium egg after sterilization is cut, stem is taken out, stem is cut into the square of several 0.5～1 centimeter sides, every test tube connects
1 piece of square is planted, 1 piece of square of inoculation is placed in test tube bottom end, on the Talinum crassifolium slant medium of about 1/3rd test tube length,
Each bacterium egg can be inoculated with 8～12 test tubes, cover cap test tube, cap test tube one end of every test tube, wrap 2 layers of paper, encase examination completely
Pipe cap, is bound with rope, is prevented the test tube of cap test tube one end directly and air contact, is obtained final product the test tube being inoculated with；
(4) incubated
The good test tube of above-mentioned inoculation, is placed in 20 DEG C of constant incubator lucifuge culture, obtains final product within 45 days or so Mount Fanjing wild red support
Dictyophora phalloidea parent species.
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| CN106754394B (en) * | 2016-07-10 | 2021-11-12 | 贵州省农作物品种资源研究所 | Dictyophora rubrovalvata yzs020 strain suitable for substitute cultivation |
| CN110637681A (en) * | 2018-06-27 | 2020-01-03 | 贵州金蟾大山生物科技有限责任公司 | Method for culturing Dictyophora rubrovalvata mother strain |
| CN111194663A (en) * | 2019-03-26 | 2020-05-26 | 贵州久源顺生态农业开发有限责任公司 | Dictyophora thermosphakii cultivation method |
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| CN111903432B (en) * | 2020-08-06 | 2022-04-01 | 贵州珍稀食用菌科技有限公司 | Dictyophora rubrovalvata strain rapid propagation method and application thereof |
| CN112586273A (en) * | 2020-12-17 | 2021-04-02 | 贵州丰源现代农业有限公司 | Culture medium, preparation method thereof, Dictyophora rubrovalvata mother strain culture method and application |
| CN112931058B (en) * | 2021-01-29 | 2022-10-11 | 杭州市农业科学研究院 | Separation and purification method of Moganshan wild Dictyophora rubrovalvata strain |
| CN115404171A (en) * | 2022-08-31 | 2022-11-29 | 贵州金荪生物科技开发有限责任公司 | Preparation method of Dictyophora rubrovalvata liquid mother strain |
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