CN104604522A - Method for producing cordyceps militaris - Google Patents
Method for producing cordyceps militaris Download PDFInfo
- Publication number
- CN104604522A CN104604522A CN201510023804.8A CN201510023804A CN104604522A CN 104604522 A CN104604522 A CN 104604522A CN 201510023804 A CN201510023804 A CN 201510023804A CN 104604522 A CN104604522 A CN 104604522A
- Authority
- CN
- China
- Prior art keywords
- test tube
- medium
- inoculation
- cordyceps militaris
- liquid production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention provides a method for producing cordyceps militaris. The method is characterized by including the steps of culturing medium preparing; split charging; sterilizing and cooling; inoculating; cultivating; inspecting; finished product yielding. According to the technology of the method, the cordyceps militaris is manually bred and cultivated, the problems that bacteria are carried on silkworm chrysalises and the variety is degraded are solved, the mycelial growth speed is high, the silkworm-chrysalis cordyceps militaris growth speed is high, the success rate is high, the cordyceps militaris is high in yield and good in color and luster, the requirement of the market is met, industrialized production is achieved through manual domestication, broad prospects are opened up for developing and using the famous and precious traditional Chinese medicinal materials, and the high economic benefits are brought to broad producers.
Description
Technical field
The present invention relates to Cordyceps militaris, be specifically related to a kind of Cordyceps militaris production method.
Background technology
The weather conditions being distributed with its uniqueness of wild Chinese caterpillar fungus and geographical environment, growth district and output are extremely restricted.At present, along with to the further investigation of Chinese caterpillar fungus health product and exploitation, wild Chinese caterpillar fungus can not meet the growing requirement of people far away.Therefore by manually carrying out breeding and the cultivation of Chinese caterpillar fungus, the needs in market have been catered to.Now, researcher has successfully been bred and has been cultivated Cordyceps militaris in lepidopterous insects.No matter the Cordyceps militaris of artificial cultivation is chemical composition, or pharmacological action is all distinguished very micro-with wild Chinese caterpillar fungus, completely can product as an alternative.Therefore, by domestication, factorial praluction, for the exploitation of this rare traditional Chinese medicine open bright prospects.
, as the hometown of Zhang Zhongjing, early there is the title of " three step one medicines " in Nanyang.In recent years; Xixia dogwood, Nan Zhao's flower bud of lily magnolia, the abundant red sage root of mahjong, Tanghe cape jasmine and the tuber of pinellia 5 production base of traditional Chinese medicines successively obtain national protection of place of origin certification; but also very limited as the exploitation of medicinal fungus, the experimental study of Cordyceps militaris or blank, say nothing of and developed.Therefore study the production method of Cordyceps militaris, particularly important for the Nanyang have " Hua Yaozhi township ", it not only enriches the medicinal material kind in Liao Wo city, also can make positive contribution for economic development.
Summary of the invention
Technical problem to be solved by this invention is a kind of Cordyceps militaris production method of design, utilizes the method to pass through manually to carry out breeding and the cultivation of Chinese caterpillar fungus, can be the producer and bring higher economic benefit.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of Cordyceps militaris production method, comprises following methods:
Medium preparation → packing → sterilizing cooling → inoculation → cultivation → inspection → finished product, wherein:
Medium is prepared, point solid mother culture media and liquid production kind medium;
Solid Mother culture based formulas is:
(1). potato 200.0g, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, Cobastab
110.0mg, agar 20.0g, water 1000ml, pH value 6.0 ~ 7.0;
(2). glucose 100.0g, peptone 10.0g, yeast extract 1.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, agar 20.0g, water 1000ml, pH value 6.0 ~ 7.0;
Liquid production kind culture medium prescription is:
(1). potato 200.0g, dried silkworm chrysalis meal 10.0g, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, Cobastab
150 mg, water 1000ml, pH value 6.0 ~ 7.0;
(2). glucose 100.0g, peptone 10.0g, yeast extract 1.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, agar 20.0g, water 1000ml, pH value 6.0 ~ 7.0;
Packing, for solid mother culture media, weighs up various raw material by formula, potato is cleaned peeling and removes eye, take 200g, be cut into 3 ~ 5mm thin slice, add water 1000ml, and slow fire boils 20 ~ 30 minutes, by 4 layers of filtered through gauze, filtrate is put back in pot, adds agar and fully dissolves, then adds other composition successively, after stirring and dissolving, add boiling water and complement to 1000ml, while hot packing test tube, loading amount is 1/4 ~ 1/5 of test tube, fills in plug;
For liquid production kind medium, weigh up various raw material by formula, peeling potatoes is removed eye, take 200g, be cut into the thin slice that 3 ~ 5mm is thick, put into pot together with nutrient matrix, slow fire boils 20 ~ 30 minutes, by 8 layers of filtered through gauze, filtrate complements to 1000 milliliters with boiling water, then adds other composition successively, stirring and dissolving, then load in glass infusion bottle, liquid loading amount is no more than 2/5 of container total measurement (volume), and bottleneck polyacrylic film and latex circle seal;
Sterilizing cools, and for solid mother culture media, high pressure steam sterilization 0.11MPa, keeps 30 minutes, when Pressure Drop is to " 0 ", opens air valve, takes out sterilizing thing, is put into inclined-plane;
For liquid production kind medium, adopt high pressure steam sterilization, high pressure steam sterilization 0.11MPa, keeps 30 minutes, when the pressure of sterilizer reduces to zero, opens sterilizer, takes out seed bottle, can inoculate after seed bottle is cooled to room temperature;
Inoculation, for solid mother culture media, puts into inoculating hood by medium to be seeded, puts into inoculating tool simultaneously and carry out disinfection, and combined with ultraviolet radiation irradiates and carries out space disinfection 40 minutes simultaneously; The tampon of test tube to be managed together or bottleneck cuts off, clean outer wall with 75% alcohol swab, after inoculating tool is burnt on alcolhol burner flame, be placed in clean sky and in vitro cool for subsequent use; Test tube stock be placed on extension clamp, rotaryly take off tampon, right hand end plays alcolhol burner, allows the flame contact mouth of pipe, and test tube is slowly rotated 3 ~ 4 weeks by left hand, then by test tube fix tightly; Test tube mouth will control operating within the scope of Alcohol Flame 10cm; With the inoculation hook for subsequent use after sterilization, removing female kind tip portion on inclined-plane and the mother of central authorities plant block, subsequently inoculation hook are put back to female kind test tube; Left hand holds the test-tube culture medium of pre-vaccination, the mouth of pipe is slightly toward having a down dip, hold inoculation hook with right hand little finger and the third finger, forefinger and middle finger, plant from mother and in vitro hook up the kind block that diameter is about 2mm size, put into pre-vaccination medium slant central authorities, extract inoculation hook out, put back to and female plant test tube, gently burn tampon that the right hand is held, rotaryly fill in the test tube taking over bacterium, be put into appropriate location by the right hand, complete all pre-vaccination test tubes continuously;
For liquid production kind medium, under aseptic technique, to plant inclined-plane from mother with inoculation hook and cut 1cm2 bacterium face and put into strain cultivation bottle, rapid polyacrylic film and latex circle envelope prick bottleneck, often prop up mother's kind and can inoculate 10 ~ 15 bottles of liquid production kinds;
Cultivate, plant cultivation temperature 18 ~ 23 DEG C, plant and cultivate humidity 60 ~ 65%, Spawn incubation light is dark condition, and Spawn incubation air is environment improve free from extraneous odour;
For solid mother culture media, solid mother plants and covers with test tube in 7 ~ 10 days;
For liquid production kind medium, the production of liquid production kind, main employing shaking table shaken cultivation, shake frequency suitably to regulate according to shaking table performance and bacterial classification character, the bacterial classification Peloton density that requirement is cultivated greatly, mycelia extension bottle wall is few, cultivation cycle is short and the rear colonization ability of inoculation is strong, a bacterium is fast; Reciprocal shaker 60 ~ 70 beats/min, rotary shaker 100 ~ 120 revs/min, the shaken cultivation time is 4 ~ 6 days; Do not have the adopted static gas wave refrigerator of appointed condition, the static gas wave refrigerator time is 7 ~ 10 days;
Check, make regular check between culture period, general every day checks 2 times, eliminates microbiological contamination or inferior position bacterial classification in time;
Finished product, finally goes out finished product.
Wherein, described nutrient matrix is potato, corn flour, dried silkworm chrysalis meal, soybean meal, bean cake powder, glucose, peptone, yeast extract (powder), potassium dihydrogen phosphate, magnesium sulfate, Cobastab
1for raw material, through formulated.
The present invention adopts the Cordyceps militaris production method designed by technique scheme, by manually carrying out breeding and the cultivation of Chinese caterpillar fungus, the needs in market are catered to, pass through domestication, factorial praluction, for the exploitation of this rare traditional Chinese medicine open bright prospects, higher economic benefit is brought to the numerous producers.
Accompanying drawing explanation
Fig. 1 represents the schematic flow sheet of Cordyceps militaris production method of the present invention.
Embodiment
Below in conjunction with accompanying drawing, Cordyceps militaris production method of the present invention is illustrated.
Cordyceps militaris production method of the present invention potato, corn flour, dried silkworm chrysalis meal, soybean meal, bean cake powder, glucose, peptone, yeast extract (powder), potassium dihydrogen phosphate, magnesium sulfate, Cobastab
1deng being raw material, as the nutrient matrix of Cordceps militaris after preparation.Wherein, corn flour, dried silkworm chrysalis meal, soybean meal, bean cake powder are commercially available prod, require without caking without rotten; Potato goes out green can not the using of bud mutation; Peptone, yeast extract (powder) are biochemical reagents; Glucose, potassium dihydrogen phosphate, magnesium sulfate are chemical pure higher level reagent; Agar, Cobastab
1for the above product of food-grade; Water is the drinkable water such as running water.
The culture vessel that Cordyceps militaris production method of the present invention uses is:
1. solid mother plants and produces container, and use teat glass and plug, test tube specification is 18 × 180mm or 20 × 200mm, and test tube plug generally adopts plug, the holding time is short be directly used in liquid production kind can use tampon, absorbent cotton can not be used to do test tube plug.
2. liquid production kind produces container, uses 250ml, 500ml glass infusion bottle, with polyacrylic film and the sealing of latex circle.
See Fig. 1, Cordyceps militaris production method of the present invention comprises following methods:
Medium preparation → packing → sterilizing cooling → inoculation → cultivation → inspection → finished product, wherein:
Medium is prepared, point solid mother culture media and liquid production kind medium;
Solid Mother culture based formulas is:
(1). potato 200.0g, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, Cobastab
110.0mg, agar 20.0g, water 1000ml, pH value 6.0 ~ 7.0;
(2). glucose 100.0g, peptone 10.0g, yeast extract 1.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, agar 20.0g, water 1000ml, pH value 6.0 ~ 7.0;
Liquid production kind culture medium prescription is:
(1). potato 200.0g, dried silkworm chrysalis meal 10.0g, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, Cobastab
150 mg, water 1000ml, pH value 6.0 ~ 7.0;
(2). glucose 100.0g, peptone 10.0g, yeast extract 1.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, agar 20.0g, water 1000ml, pH value 6.0 ~ 7.0;
Packing, for solid mother culture media, weighs up various raw material by formula, potato is cleaned peeling and removes eye, take 200g, be cut into 3 ~ 5mm thin slice, add water 1000ml, and slow fire boils 20 ~ 30 minutes, by 4 layers of filtered through gauze, filtrate is put back in pot, adds agar and fully dissolves, then adds other composition successively, after stirring and dissolving, add boiling water and complement to 1000ml, while hot packing test tube, loading amount is 1/4 ~ 1/5 of test tube, fills in plug;
For liquid production kind medium, various raw material is weighed up by formula, peeling potatoes is removed eye, take 200g, be cut into the thin slice that 3 ~ 5mm is thick, with dried silkworm chrysalis meal (or other fill a prescription in the agricultural product raw material such as corn flour, soybean meal, bean cake powder) together with put into pot, slow fire boils 20 ~ 30 minutes, by 8 layers of filtered through gauze, filtrate complements to 1000 milliliters with boiling water, then adds other composition successively, stirring and dissolving, then load in glass infusion bottle, liquid loading amount is no more than 2/5 of container total measurement (volume), and bottleneck polyacrylic film and latex circle seal;
Sterilizing cools, and for solid mother culture media, high pressure steam sterilization 0.11MPa, keeps 30 minutes, when Pressure Drop is to " 0 ", opens air valve, takes out sterilizing thing, is put into inclined-plane;
For liquid production kind medium, adopt high pressure steam sterilization, high pressure steam sterilization 0.11MPa, keeps 30 minutes, when the pressure of sterilizer reduces to zero, opens sterilizer, takes out seed bottle, can inoculate after seed bottle is cooled to room temperature;
Inoculation, for solid mother culture media, puts into inoculating hood by medium to be seeded, puts into inoculating tool used simultaneously, carry out disinfection with chemicals.Combined with ultraviolet radiation irradiates and carries out space disinfection 40 minutes simultaneously; Managed together by the tampon of test tube or bottleneck cuts off, clean outer wall with 75% alcohol swab, after being burnt on alcolhol burner flame by inoculating tool (hook, shovel, cutter etc.), being placed in clean sky in vitro cools for subsequent use; Test tube stock be placed on extension clamp, rotaryly take off tampon, right hand end plays alcolhol burner, allows the flame contact mouth of pipe, and test tube is slowly rotated 3 ~ 4 weeks by left hand, then by test tube fix tightly; Test tube mouth will control operating within the scope of Alcohol Flame 10cm; With the inoculation hook for subsequent use after sterilization, removing female kind tip portion on inclined-plane and the mother of central authorities plant block, subsequently inoculation hook are put back to female kind test tube; Left hand holds the test-tube culture medium of pre-vaccination, the mouth of pipe is slightly toward having a down dip, hold inoculation hook with right hand little finger and the third finger, forefinger and middle finger, plant from mother and in vitro hook up the kind block that diameter is about 2mm size, put into pre-vaccination medium slant central authorities, extract inoculation hook out, put back to and female plant test tube, gently burn tampon that the right hand is held, rotaryly fill in the test tube taking over bacterium, be put into appropriate location by the right hand, complete all pre-vaccination test tubes continuously;
For liquid production kind medium, under aseptic technique, to plant inclined-plane from mother with inoculation hook and cut 1cm2 bacterium face (slightly can be with medium) and put into strain cultivation bottle, rapid polyacrylic film and latex circle envelope prick bottleneck, often prop up female kind and can inoculate 10 ~ 15 bottles of liquid production kinds;
Cultivate, plant cultivation temperature 18 ~ 23 DEG C, plant and cultivate humidity 60 ~ 65%, Spawn incubation light is dark condition, and Spawn incubation air is environment improve free from extraneous odour;
For solid mother culture media, solid mother plants and covers with test tube in 7 ~ 10 days;
For liquid production kind medium, the production of liquid production kind, main employing shaking table shaken cultivation, shake frequency suitably to regulate according to shaking table performance and bacterial classification character, the bacterial classification Peloton density that requirement is cultivated greatly, mycelia extension bottle wall is few, cultivation cycle is short and the rear colonization ability of inoculation is strong, a bacterium is fast; Reciprocal shaker 60 ~ 70 beats/min, rotary shaker 100 ~ 120 revs/min, the shaken cultivation time is 4 ~ 6 days; Do not have the adopted static gas wave refrigerator of appointed condition, the static gas wave refrigerator time is 7 ~ 10 days;
Check, make regular check between culture period, general every day checks 2 times, eliminates microbiological contamination or inferior position bacterial classification in time;
Finished product, finally goes out finished product.
Inventor has found out the cordyceps bacterial classification adapting to city's environmental condition, has introduced four bacterial classifications simultaneously, finds out this bacterial classification exhibits excellent of CM-23, can promote the use of by test.
The present invention uses rice, wheat mixed culture medium production effect good.Cordyceps militaris is normally cooked medium with rice, and also useful wheat is cooked medium.Inventor adopts rice through overtesting, wheat (rice accounts for 2/3, and wheat accounts for 1/3) mixed culture medium is produced, and shows out carelessness higher, fruit body shapeliness, coloury performance, and second batch goes out grass better, and commodity value is high, is suitable for applying.
The present invention, to tussah chrysalis disinfection inoculation, solves in pupal cell problem of carrying disease germs.General silkworm chrysalis is produced Cordyceps militaris and is mostly to adopt chemical disinfection method, only solves a pupal cell surface and to carry disease germs problem, cause success rate lower.Inventor adopts rice to do the end and puts into the damp and hot autoclaving of silkworm chrysalis, and sterilizing is thorough, and solve silkworm chrysalis and to carry disease germs problem, mycelial growth is rapid, and it is fast that silkworm chrysalis goes out grass, and success rate is high, and Cordyceps militaris output is high, color and luster good.
The present invention is to the problem of Cordyceps militaris rejuvenation.Cordyceps militaris spawn is very easily degenerated because of environmental problem premium properties, and inventor adopts through test of many times that tissue is separated, spore method of being scattered carries out rejuvenation to bacterial classification, solves deterioration of variety problem.
Claims (2)
1. a Cordyceps militaris production method, is characterized in that comprising following methods:
Medium preparation → packing → sterilizing cooling → inoculation → cultivation → inspection → finished product, wherein:
Medium is prepared, point solid mother culture media and liquid production kind medium;
Solid Mother culture based formulas is:
(1). potato 200.0g, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, Cobastab
110.0mg, agar 20.0g, water 1000ml, pH value 6.0 ~ 7.0;
(2). glucose 100.0g, peptone 10.0g, yeast extract 1.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, agar 20.0g, water 1000ml, pH value 6.0 ~ 7.0;
Liquid production kind culture medium prescription is:
(1). potato 200.0g, dried silkworm chrysalis meal 10.0g, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, Cobastab
150 mg, water 1000ml, pH value 6.0 ~ 7.0;
(2). glucose 100.0g, peptone 10.0g, yeast extract 1.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, agar 20.0g, water 1000ml, pH value 6.0 ~ 7.0;
Packing, for solid mother culture media, weighs up various raw material by formula, potato is cleaned peeling and removes eye, take 200g, be cut into 3 ~ 5mm thin slice, add water 1000ml, and slow fire boils 20 ~ 30 minutes, by 4 layers of filtered through gauze, filtrate is put back in pot, adds agar and fully dissolves, then adds other composition successively, after stirring and dissolving, add boiling water and complement to 1000ml, while hot packing test tube, loading amount is 1/4 ~ 1/5 of test tube, fills in plug;
For liquid production kind medium, weigh up various raw material by formula, peeling potatoes is removed eye, take 200g, be cut into the thin slice that 3 ~ 5mm is thick, put into pot together with nutrient matrix, slow fire boils 20 ~ 30 minutes, by 8 layers of filtered through gauze, filtrate complements to 1000 milliliters with boiling water, then adds other composition successively, stirring and dissolving, then load in glass infusion bottle, liquid loading amount is no more than 2/5 of container total measurement (volume), and bottleneck polyacrylic film and latex circle seal;
Sterilizing cools, and for solid mother culture media, high pressure steam sterilization 0.11MPa, keeps 30 minutes, when Pressure Drop is to " 0 ", opens air valve, takes out sterilizing thing, is put into inclined-plane;
For liquid production kind medium, adopt high pressure steam sterilization, high pressure steam sterilization 0.11MPa, keeps 30 minutes, when the pressure of sterilizer reduces to zero, opens sterilizer, takes out seed bottle, can inoculate after seed bottle is cooled to room temperature;
Inoculation, for solid mother culture media, puts into inoculating hood by medium to be seeded, puts into inoculating tool simultaneously and carry out disinfection, and combined with ultraviolet radiation irradiates and carries out space disinfection 40 minutes simultaneously; The tampon of test tube to be managed together or bottleneck cuts off, clean outer wall with 75% alcohol swab, after inoculating tool is burnt on alcolhol burner flame, be placed in clean sky and in vitro cool for subsequent use; Test tube stock be placed on extension clamp, rotaryly take off tampon, right hand end plays alcolhol burner, allows the flame contact mouth of pipe, and test tube is slowly rotated 3 ~ 4 weeks by left hand, then by test tube fix tightly; Test tube mouth will control operating within the scope of Alcohol Flame 10cm; With the inoculation hook for subsequent use after sterilization, removing female kind tip portion on inclined-plane and the mother of central authorities plant block, subsequently inoculation hook are put back to female kind test tube; Left hand holds the test-tube culture medium of pre-vaccination, the mouth of pipe is slightly toward having a down dip, hold inoculation hook with right hand little finger and the third finger, forefinger and middle finger, plant from mother and in vitro hook up the kind block that diameter is about 2mm size, put into pre-vaccination medium slant central authorities, extract inoculation hook out, put back to and female plant test tube, gently burn tampon that the right hand is held, rotaryly fill in the test tube taking over bacterium, be put into appropriate location by the right hand, complete all pre-vaccination test tubes continuously;
For liquid production kind medium, under aseptic technique, to plant inclined-plane from mother with inoculation hook and cut 1cm2 bacterium face and put into strain cultivation bottle, rapid polyacrylic film and latex circle envelope prick bottleneck, often prop up mother's kind and can inoculate 10 ~ 15 bottles of liquid production kinds;
Cultivate, plant cultivation temperature 18 ~ 23 DEG C, plant and cultivate humidity 60 ~ 65%, Spawn incubation light is dark condition, and Spawn incubation air is environment improve free from extraneous odour;
For solid mother culture media, solid mother plants and covers with test tube in 7 ~ 10 days;
For liquid production kind medium, the production of liquid production kind, main employing shaking table shaken cultivation, shake frequency suitably to regulate according to shaking table performance and bacterial classification character, the bacterial classification Peloton density that requirement is cultivated greatly, mycelia extension bottle wall is few, cultivation cycle is short and the rear colonization ability of inoculation is strong, a bacterium is fast; Reciprocal shaker 60 ~ 70 beats/min, rotary shaker 100 ~ 120 revs/min, the shaken cultivation time is 4 ~ 6 days; Do not have the adopted static gas wave refrigerator of appointed condition, the static gas wave refrigerator time is 7 ~ 10 days;
Check, make regular check between culture period, general every day checks 2 times, eliminates microbiological contamination or inferior position bacterial classification in time;
Finished product, finally goes out finished product.
2. Cordyceps militaris production method according to claim 1, is characterized in that described nutrient matrix is potato, corn flour, dried silkworm chrysalis meal, soybean meal, bean cake powder, glucose, peptone, yeast extract (powder), potassium dihydrogen phosphate, magnesium sulfate, Cobastab
1for raw material, through formulated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510023804.8A CN104604522A (en) | 2015-01-19 | 2015-01-19 | Method for producing cordyceps militaris |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510023804.8A CN104604522A (en) | 2015-01-19 | 2015-01-19 | Method for producing cordyceps militaris |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104604522A true CN104604522A (en) | 2015-05-13 |
Family
ID=53139401
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510023804.8A Pending CN104604522A (en) | 2015-01-19 | 2015-01-19 | Method for producing cordyceps militaris |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104604522A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706742A (en) * | 2016-04-18 | 2016-06-29 | 程致远 | Culture method of cordyceps militaris shells and shell cordyceps militaris |
| CN106701597A (en) * | 2016-12-15 | 2017-05-24 | 防城港市蓝瀚达科技有限公司 | Manufacturing method of cucumber agar slant culture medium |
| CN108812051A (en) * | 2018-05-23 | 2018-11-16 | 鲁东大学 | A kind of cordyceps culturing method |
| CN111758486A (en) * | 2020-07-13 | 2020-10-13 | 江西菌草生物科技有限公司 | Large-scale high-yield production and cultivation method for early-maturing cordyceps militaris of tussah |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100922311B1 (en) * | 2009-06-10 | 2009-10-21 | 이태봉 | Methods of culturing Inonotus obliquus, Phellinus linteus, Ganoderma lucidum, Sparassis crispa and Vegetable Worms for production of substances containing AHCC |
| KR20100104408A (en) * | 2009-03-17 | 2010-09-29 | 주식회사머쉬텍 | Cordyceps militaris mycellia cultured by using rice and poultry soup for health using the smae |
| CN103460981A (en) * | 2013-07-24 | 2013-12-25 | 山西省医药与生命科学研究院 | Novel cordyceps militaris culturing method |
| CN103733875A (en) * | 2013-11-08 | 2014-04-23 | 吉林省农业科学院 | Cordyceps militaris factory production method and process |
| CN104160873A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Cultivation and management method for cordyceps militaris |
| CN104170650A (en) * | 2014-08-14 | 2014-12-03 | 安徽亿源中药饮片科技有限公司 | Cordyceps militaris industrialized artificial cultivation method |
-
2015
- 2015-01-19 CN CN201510023804.8A patent/CN104604522A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100104408A (en) * | 2009-03-17 | 2010-09-29 | 주식회사머쉬텍 | Cordyceps militaris mycellia cultured by using rice and poultry soup for health using the smae |
| KR100922311B1 (en) * | 2009-06-10 | 2009-10-21 | 이태봉 | Methods of culturing Inonotus obliquus, Phellinus linteus, Ganoderma lucidum, Sparassis crispa and Vegetable Worms for production of substances containing AHCC |
| CN103460981A (en) * | 2013-07-24 | 2013-12-25 | 山西省医药与生命科学研究院 | Novel cordyceps militaris culturing method |
| CN103733875A (en) * | 2013-11-08 | 2014-04-23 | 吉林省农业科学院 | Cordyceps militaris factory production method and process |
| CN104160873A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Cultivation and management method for cordyceps militaris |
| CN104170650A (en) * | 2014-08-14 | 2014-12-03 | 安徽亿源中药饮片科技有限公司 | Cordyceps militaris industrialized artificial cultivation method |
Non-Patent Citations (3)
| Title |
|---|
| 支支81: "接种", 《百度百科》 * |
| 汪西强等: "蝉蛹人工培育蛹虫草方法的研究", 《安徽农业科学》 * |
| 葫芦岛市质量技术监督局: "北虫草菌种生产技术规程", 《辽宁省地方标准DB21/T 1730—2009》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706742A (en) * | 2016-04-18 | 2016-06-29 | 程致远 | Culture method of cordyceps militaris shells and shell cordyceps militaris |
| CN105706742B (en) * | 2016-04-18 | 2018-10-02 | 程致远 | A kind of cultural method of cordyceps sinensis shellfish and shellfish cordyceps sinensis |
| CN106701597A (en) * | 2016-12-15 | 2017-05-24 | 防城港市蓝瀚达科技有限公司 | Manufacturing method of cucumber agar slant culture medium |
| CN108812051A (en) * | 2018-05-23 | 2018-11-16 | 鲁东大学 | A kind of cordyceps culturing method |
| CN108812051B (en) * | 2018-05-23 | 2021-01-05 | 鲁东大学 | Cordyceps militaris culture method |
| CN111758486A (en) * | 2020-07-13 | 2020-10-13 | 江西菌草生物科技有限公司 | Large-scale high-yield production and cultivation method for early-maturing cordyceps militaris of tussah |
| CN111758486B (en) * | 2020-07-13 | 2021-11-23 | 江西菌草生物科技有限公司 | Large-scale high-yield production and cultivation method for early-maturing cordyceps militaris of tussah |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103563655B (en) | Cultivate the technique of live body nymphosis CORDYCEPS | |
| CN104651239B (en) | A kind of promotion dendrobium devonianum seeds sprout the bacterial strain to form seedling and its application | |
| CN104920065B (en) | A kind of wild Dictyophora rubrovalvata parent species preparation method of Mount Fanjing | |
| CN104054513B (en) | Coragyceps militaris bacterium culture method | |
| CN102138436A (en) | Method for culturing selenium-enriched Cordyceps militaris | |
| CN104322451A (en) | Manual low-altitude raising method for host insect hepialidae of ophiocordyceps sinensis | |
| CN101491229B (en) | Vitro culturing method of pearl shell mantle tissue and culture medium | |
| CN103283608B (en) | Factory cultivation strains of needle mushrooms, and cultivation method thereof | |
| CN104604522A (en) | Method for producing cordyceps militaris | |
| CN103571756B (en) | Test tube screening method of isaria cicadae strain and culture medium | |
| CN108401794A (en) | A kind of armillaria mellea accreting with Rhizoma Gastrodiae liquid spawn production method and cultigen special culture media | |
| CN101654665B (en) | Method for preparing Bacillus subtilis | |
| CN101897270A (en) | Production technology of Cordyceps sinensis mycelium | |
| CN107586725B (en) | Cordyceps liquid culture medium and method for culturing cordyceps by using same | |
| CN104756754B (en) | A kind of cultural method of Antrodia camphorata fructification | |
| CN105779372A (en) | Potato culture medium suitable for ustilaginoidea virens spore production and application thereof | |
| CN102786334A (en) | Culture medium for culturing edible fungus production mother seeds | |
| CN103210787B (en) | Cordyceps militaris, egged cordyceps sinensis, culturing method and application of egged cordyceps sinensis | |
| CN110214702A (en) | Dendrobidium huoshanness tissue-cultured seedling is cultivated and hardening off method | |
| CN106676018A (en) | Agaricus bisporus strain breeding method applicable to standardized plant | |
| CN103583233A (en) | Preparation method for reduction type liquid spawn | |
| CN105602857B (en) | A kind of optimization method that wild cicada fungus liquid spawn is manually cultivated | |
| CN104823716A (en) | Culture and preparing method of fungus symbiotic hypha powder | |
| CN104230566B (en) | A kind of mycelial culture medium of fast culture Armillaria luteo-virens and cultural method | |
| CN106350456A (en) | Manufacture and detection method of lucid ganoderma liquid strains |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150513 |