CN106676018A - Agaricus bisporus strain breeding method applicable to standardized plant - Google Patents
Agaricus bisporus strain breeding method applicable to standardized plant Download PDFInfo
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- CN106676018A CN106676018A CN201611264852.7A CN201611264852A CN106676018A CN 106676018 A CN106676018 A CN 106676018A CN 201611264852 A CN201611264852 A CN 201611264852A CN 106676018 A CN106676018 A CN 106676018A
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Abstract
The invention discloses an agaricus bisporus strain breeding method applicable to a standardized plant. An agaricus bisporus strain is taken as a mother strain for breeding, a fruiting experiment is performed, stock seeds are prepared, triticale is selected as a raw material, the raw material is soaked in a solution with the pH value ranging from 11.0 to 13.0 and stirred to adjust the pH value to range from 7.0 to 9.0, half of a bag of the raw material is quantitively packaged with a sterile bag, a lantern ring and a breather plug are used for sealing, sterilization is performed at the high temperature of 120-130 DEG C under the pressure of 0.10-0.20 MPa for 2-3 h, sterile inoculation is performed after cooling, and the agaricus bisporus strain is cultured at the constant temperature. By means of the method, high-quality, pure and high-yield agaricus bisporus cultivars can be bred in batches, the breeding cycle is shortened greatly, the pollution probability is reduced, the general breeding time of the cultivars lasts 15 days to 18 days, the water content of bred strain grains is consistent, the biofilm growing phenomenon is avoided, the qualification rate can reach 96%, and the method has broad practicability in strain breeding fields.
Description
Technical field
The invention belongs to the technical field of fungus growing technique, more specifically, the present invention relates to a kind of be applied to standard
The technical field of the Agaricus Bisporus strain mating system in chemical plant.
Background technology
Edible fungi is the health food of human future, and artificial implantation edible fungi has become an emerging agricultural modernization
Branch.In terms of Agro-ecology circulation, domestic fungus cultivating occupies critically important position.At present, mushroom industryization is national
Greatly develop.The principal item of edible fungi is straw rotting fungus Agaricus Bisporus (Agaricus bisporus), and protein content is high, fresh
Tender good to eat, by the whole world, people likes, in terms of artificial implantation edible fungi, occupies 80% artificial culture's proportion, is edible fungi
The main product of class.
At present, artificial local method is supported and plants Agaricus Bisporus traditional mode towards half batch production, factorial praluction Agaricus Bisporus pattern
Transition.One by one the Agaricus Bisporus factory of standard emerge rapidly in large numbersBamboo shoots after a spring rain as rise, however, the Agaricus Bisporus factory just gone into operation and
Speech, yield is not high, and quality is general, and pest and disease damage incidence rate is high.Economic benefit or loss, or loss is on the verge of, trace it to its cause, affect me
One key problem of state's Agaricus Bisporus industry is that our country's technical matters in terms of Agaricus Bisporus breeding strain fall behind, neither one
The Agaricus Bisporus strain plant of standard, production is inferior with strain quality, Character instability.In Agaricus Bisporus Yang Zhi enterprises, except few portion
Division of labor factory is introduced beyond expensive strain from external, and most of Agaricus Bisporus are cultivated enterprise and still continue to use traditional workshop-based strain
The strain that factory cultivates.There is following defect in workshop-based cultivation Agaricus Bisporus strain：One is that culture matrix container typically all adopts 500ML
Vial, parent species access after this container, slowly can only grow to lower end from the upper end of bottleneck, cover with bacterium bottle take 35 days-
40 days.Mycelia in seed bottle is gradually aging from lower to upper, and cell age is inconsistent, and vitality power has different, and local ageing is serious, resists
Condition of disease power is reduced；Two is that medium sterilization is not thorough, the inoculation of local method wooden case, and Medium Culture is often hidden cause of disease, worm substance, in air
Cultivate under non-purification state, pollution rate is increased, strain makes and is difficult to pure culture；Three is that strain soaks, the matrix granule of steaming and decocting is received
Heat, immersion degree are uneven, and water content is inconsistent, mycelium growth vigor unshapeliness, humidity high breakage of particles caking, poor air permeability, bacterium
Silk poor growth, disease resistance is poor.Therefore, the stable complete Agaricus Bisporus strain agriculture of a kind of high-quality, high production capacity, biological character is needed badly
Industry industrialization cultivation systems.
The content of the invention
For the not enough of state of the art and the problem for existing, especially traditional workshop-based Agaricus Bisporus strain quality
Low, Character instability, affects the bottleneck problem of China's Agaricus Bisporus industry development, in order to solve the above problems, the invention provides
A kind of Agaricus Bisporus strain mating system suitable for standard chemical plant, compared with prior art, the method can be numerous in large quantity
Bring out that high-quality is pure, high yield Agaricus Bisporus cultigen, more traditional breeding method cultivation period was compared for 30-45 days and significantly shortened
Cultivation period, while reducing pollution probability, cultigen typically cultivates the time for -18 days 15 days, without steaming and decocting black Semen Tritici aestivi, section
The about energy, the strain grain water content cultivated is consistent, and long bacterium does not occur by phenomenon, qualification rate up to 96%, due to breeding three-level
Plant and select two grades of kinds, no matter doing solid spawn with grain, still use liquid spawn, they all can be purified in rank environment at ten grades
It is uniformly blended into or is sprayed in black Semen Tritici aestivi culture medium, growing point is more, is sprouted fast, with what is be subject on each granule of a collection of substrate
Inoculation time is unanimous on the whole, so the time point of initial growth is consistent, cell age is consistent, and cell age is short, and strain vitality is strong, mycelia point
Cloth uniformity specification, has wide applicability in strainsfor cultivation field.
A kind of Agaricus Bisporus strain mating system suitable for standard chemical plant that the present invention is provided, specifically using following technology
Step：
(1) prepared by original seed：High-quality is selected, the Agaricus Bisporus strain for flushing makees parent species selection-breeding, and does fruiting experiment, selects
Cultivating rate height, good mushroom quality, production capacity are in 30Kg/m2Bacterial strain above is used as Agaricus Bisporus strain parent species；Agaricus Bisporus strain parent species are adopted
Mycelia carrier being done to Semen setariae, Agaricus Bisporus strain solid original seed or configuration nutritional solution are made Jing after breading protospecies in liquid spawn
Fermentation tank makes Agaricus Bisporus strain liquid original seed Jing after breading protospecies.
(2) material choice：Black Semen Tritici aestivi is screened, grain, flat grain and impurity is removed brokenly, is selected clean, it is full, completely,
It is fresh, without the black Semen Tritici aestivi for going mouldy.
(3) raw material soaking：Foam material pond discharges water, and dissolving quick lime makes pH value reach 11.0-13.0, mix homogeneously, foam material cage
In being placed in foam material pond, black Semen Tritici aestivi is put in foam material cage, is stirred with compressed air, 4 times a day -6 times, make black Semen Tritici aestivi water suction equal
It is even.
(4) raw material stirring：Test black Semen Tritici aestivi water content reaches standard, and with hanging foam material cage is proposed, control water is to without obvious water
Drop, pours agitator into, adds the Calcium Carbonate of 1% mass fraction and the calcium sulfate of 0.8% mass fraction, stirs, and makes pH value
7.0-9.0 is adjusted to, stirring is completed.
(5) quantitative package：Black Semen Tritici aestivi after stirring is imported into conveyer belt, it is fixed with sterilizing bag into automatic quantitative packing device
Half bag of amount packaging, is sealed with the collar and breather plug, swings to sterilize in go-cart, per layer 6 bag, totally 9 layers.
(6) high temperature sterilize：After sterilizing go-cart is filled, bacteria district sterilization cabinet door is opened, push sterilizing cabinet, order is arranged, gone out
Bacterium cabinet is sealed up a door, evacuation, logical steam sterilization, 120 DEG C -130 DEG C of sterilizing parameter, and 0.10MPa-0.20MPa, 2h-3h have sterilized
Into aerofluxuss blood pressure lowering treats that pressure, to zero, in clean room side hundred grades of purifying area sterilization cabinet doors is opened, and pulls out sterilizing go-cart, net at hundred grades
Changing cooling zone cooling 10h-14h makes material temperature reach 18 DEG C -25 DEG C.
(7) aseptic inoculation：Compost after cooling is delivered to ten grades of purifying areas, inoculation personnel open in operating board and sterilize
Bag breather plug, connects bacterium amount and accesses Agaricus Bisporus strain solid original seed or Agaricus Bisporus strain liquid original seed to sterilizing bag by 1%, shakes up,
Two sterilizing bags are poured in a culture bag, culture bag band respiratory membrane, are sealed with sealing machine, are spread out of culturing room and are cultivated.
(8) constant temperature culture：Ten thousand grades of purification culture room temperature 24 DEG C of constant temperature of setting, cultivate -18 days 15 days, and strain is ripe, passes
It is sent to 2 DEG C of refrigeration house storages of constant temperature.
In the present invention, foam material pond pH value is preferably 12.0 in raw material soaking step, and raw material stirring pH value is preferably 8.0, material
21.5 DEG C of temperature, sterilizing parameter is preferably 125 DEG C in high temperature sterilize step, 0.15MPa, 2.5h.
Agaricus Bisporus strain, Semen setariae, nutritional solution, liquid spawn fermentation tank, black Semen Tritici aestivi, quick lime, foam material that the present invention is adopted
Cage, hanging, agitator, Calcium Carbonate, calcium sulfate, automatic quantitative packing device, sterilizing bag, the collar, breather plug, sterilizing go-cart, sterilizing
Cabinet and culture bag can be bought by public channel or be customized.
The technical scheme provided using the present invention can reach following beneficial effect：
(1) present invention provide a kind of Agaricus Bisporus strain mating system suitable for standard chemical plant, Jing orthogonal tests and
Response phase method optimize technique, can in large quantity breed out that high-quality is pure, high yield Agaricus Bisporus cultigen, more traditional cultivation side
Method cultivation period was compared for 30-45 days and cultivation period is greatly shortened, while pollution probability is reduced, when cultigen is typically cultivated
Between be -18 days 15 days, without steaming and decocting black Semen Tritici aestivi, energy saving, the strain grain water content cultivated is consistent, long bacterium does not occur and is showed
As qualification rate is up to 96%.
(2) a kind of Agaricus Bisporus strain mating system suitable for standard chemical plant that the present invention is provided, due to breeding three-level
Plant and select two grades of kinds, no matter doing solid spawn with grain, still use liquid spawn, they all can be purified in rank environment at ten grades
It is uniformly blended into or is sprayed in black Semen Tritici aestivi culture medium, growing point is more, is sprouted fast, with what is be subject on each granule of a collection of substrate
Inoculation time is unanimous on the whole, so the time point of initial growth is consistent, cell age is consistent, and cell age is short, and strain vitality is strong, mycelia point
Cloth uniformity specification, has wide applicability in the strain field of breeding.
Description of the drawings
Fig. 1 is shown as the process chart of present invention offer.
Fig. 2 is shown as the response surface curve chart of the sterilising temp and sterilization pressure of present invention offer to qualification rate.
Fig. 3 is shown as the response surface curve chart of the sterilising temp and sterilization time of present invention offer to qualification rate.
Fig. 4 is shown as the response surface curve chart of the sterilization pressure and sterilization time of present invention offer to qualification rate.
Specific embodiment
Below in conjunction with the accompanying drawings 1-4 and embodiment, are described in further detail to the specific embodiment of the present invention, but this
It is bright to be not limited to following embodiments.
In the present invention, a kind of Agaricus Bisporus strain suitable for standard chemical plant for providing for the ease of description, the present invention
In mating system, the description of the relative position relation of each part be with reference to the accompanying drawings 1 Butut mode being described, such as：Upper,
Under, the position relationship such as left and right determines according to the Butut direction of accompanying drawing 1.
Agaricus Bisporus strain, Semen setariae, nutritional solution, liquid spawn fermentation tank, black Semen Tritici aestivi, quick lime, foam material that the present invention is adopted
Cage, hanging, agitator, Calcium Carbonate, calcium sulfate, automatic quantitative packing device, sterilizing bag, the collar, breather plug, sterilizing go-cart, sterilizing
Cabinet and culture bag can be bought by public channel or be customized.
What all material, reagent and the instrument selected in the present invention was all well known in the art, but do not limit the reality of the present invention
Apply, other some reagents well known in the art and equipment are applied both to the enforcement of implementation below of the present invention.
Embodiment one：Suitable for the Agaricus Bisporus strain mating system in standard chemical plant
A kind of Agaricus Bisporus strain mating system suitable for standard chemical plant that the present invention is provided, specifically using following technology
Step：
(1) prepared by original seed：High-quality is selected, the Agaricus Bisporus strain for flushing makees parent species selection-breeding, and does fruiting experiment, selects
Cultivating rate height, good mushroom quality, production capacity are in 30Kg/m2Bacterial strain above is used as Agaricus Bisporus strain parent species；Agaricus Bisporus strain parent species are adopted
Mycelia carrier being done to Semen setariae, Agaricus Bisporus strain solid original seed or configuration nutritional solution are made Jing after breading protospecies in liquid spawn
Fermentation tank makes Agaricus Bisporus strain liquid original seed Jing after breading protospecies.
(2) material choice：Black Semen Tritici aestivi is screened, grain, flat grain and impurity is removed brokenly, is selected clean, it is full, completely,
It is fresh, without the black Semen Tritici aestivi for going mouldy.Select black Semen Tritici aestivi to plant and cultivate mycelia carrier, compare tradition and do and kind use common wheat, its kind of skin
It is thick and tough and tensile, after immersion expansion, to be not easily broken and expose starch so as to overflow, complete form can be kept after autoclaving, contain
The water yield is up to more than 50%.
(3) raw material soaking：Foam material pond discharges water, and dissolving quick lime makes pH value reach 11.0-13.0, mix homogeneously, foam material cage
In being placed in foam material pond, black Semen Tritici aestivi is put in foam material cage, is stirred with compressed air, 4 times a day -6 times, make black Semen Tritici aestivi water suction equal
It is even.It is then the pH that improve water with adjuvant calcium oxide, makes black Semen Tritici aestivi kind skin waxiness damage layer, rapid imbibition.Simultaneously strong
Effectively prevent the existence of extraneous miscellaneous bacteria in the solution of alkalescence, also effectively kill the miscellaneous bacteria spore in black Semen Tritici aestivi of hiding,
So that black Semen Tritici aestivi reduces miscellaneous bacteria in water absorption course and carries as far as possible.The rye (Secale cereale L.) of immersion is stirred with compressed air, can effectively be mixed
Loosen rye (Secale cereale L.) so that rye (Secale cereale L.) is full and uniform in immersion to be contacted with water, reaches rye (Secale cereale L.) aqueous sufficient and uniform.So as to ensure
Mycelial growth is vigorous.Soaking technology can not only make black Semen Tritici aestivi reach optimal water content state, while successfully solving biography
System technique is operated to materials of wheat steaming and decocting, and cracky pops, it is impossible to ensure raw material integrity, easily causes living contaminantses this skills
Art problem, reduces and has even prevented the pollution in down-stream.
(4) raw material stirring：Test black Semen Tritici aestivi water content reaches standard, and with hanging foam material cage is proposed, control water is to without obvious water
Drop, pours agitator into, adds the Calcium Carbonate of 1% mass fraction and the calcium sulfate of 0.8% mass fraction, stirs, and makes pH value
7.0-9.0 is adjusted to, stirring is completed.Addition adjuvant Calcium Carbonate and calcium sulfate can effectively reduce and buffer raw material in high temperature sterilize
When pH reduce situation, ensure the pH of the raw material after sterilizing, on the other hand, and dry cow dung and wheat bran are added during traditional bacterial classification makes
Compare, can more effectively avoid miscellaneous bacteria from hiding, reduce pollution probability.
(5) quantitative package：Black Semen Tritici aestivi after stirring is imported into conveyer belt, it is fixed with sterilizing bag into automatic quantitative packing device
Half bag of amount packaging, is sealed with the collar and breather plug, swings to sterilize in go-cart, per layer 6 bag, totally 9 layers.With sterilizing bag quantitative package half
Bag, can not only reach optimal sterilization effect, while shaking a bag stage in inoculation, reach and most preferably shake up effect；Bag mouth using the collar, exhale
Plug sealing is inhaled, the quick-fried bag problem in sterilizing cabinet is not only solved, while further ensuring in the aseptic of hundred grades of cooling stages
Cooling.
(6) high temperature sterilize：After sterilizing go-cart is filled, bacteria district sterilization cabinet door is opened, push sterilizing cabinet, order is arranged, gone out
Bacterium cabinet is sealed up a door, evacuation, logical steam sterilization, 120 DEG C -130 DEG C of sterilizing parameter, and 0.10MPa-0.20MPa, 2h-3h have sterilized
Into aerofluxuss blood pressure lowering treats that pressure, to zero, in clean room side hundred grades of purifying area sterilization cabinet doors is opened, and pulls out sterilizing go-cart, net at hundred grades
Changing cooling zone cooling 10h-14h makes material temperature reach 18 DEG C -25 DEG C.High temperature sterilize step can consume energy minimum within the shortest time
In the case of, thoroughly sterilization.
(7) aseptic inoculation：Compost after cooling is delivered to ten grades of purifying areas, inoculation personnel open in operating board and sterilize
Bag breather plug, connects bacterium amount and accesses Agaricus Bisporus strain solid original seed or Agaricus Bisporus strain liquid original seed to sterilizing bag by 1%, shakes up,
Two sterilizing bags are poured in a culture bag, culture bag band respiratory membrane, are sealed with sealing machine, are spread out of culturing room and are cultivated.With exhaling
The culture bag of film is inhaled, can be in cultivation stage, effectively isolation miscellaneous bacteria invades, it is ensured that the pure culture of strain.
(8) constant temperature culture：Ten thousand grades of purification culture room temperature 24 DEG C of constant temperature of setting, cultivate -18 days 15 days, and strain is ripe, passes
It is sent to 2 DEG C of refrigeration house storages of constant temperature.
The technological process of the Agaricus Bisporus strain mating system suitable for standard chemical plant that the present invention is provided is referring to the institute of accompanying drawing 1
Show.
Embodiment two：Suitable for the Agaricus Bisporus strain mating system in standard chemical plant
High-quality is selected, the Agaricus Bisporus strain for flushing makees parent species selection-breeding, and does fruiting experiment, selects cultivating rate height, mushroom
Of fine quality, production capacity is in 30Kg/m2Bacterial strain above is used as Agaricus Bisporus strain parent species；Agaricus Bisporus strain parent species are adopted bacterium is cooked with Semen setariae
Silk carrier makes Agaricus Bisporus strain solid original seed Jing after breading protospecies or configuration nutritional solution is trained in liquid spawn fermentation tank Jing original seeds
Agaricus Bisporus strain liquid original seed is made after educating；Black Semen Tritici aestivi is screened, grain, flat grain and impurity is removed brokenly, is selected clean, satisfied
It is full, it is completely, fresh, without the black Semen Tritici aestivi for going mouldy；Foam material pond discharges water, and dissolving quick lime makes pH value reach 11.0, mix homogeneously, foam material
Cage is placed in foam material pond, and in foam material cage black Semen Tritici aestivi is put into, and is stirred with compressed air, and 4 times a day, makes black Semen Tritici aestivi water suction uniform；
Test black Semen Tritici aestivi water content reaches standard, and with hanging foam material cage is proposed, control water adds 1% to agitator without obvious water droplet, is poured into
The calcium sulfate of the Calcium Carbonate of mass fraction and 0.8% mass fraction, stirs, and makes pH value be adjusted to 7.0, and stirring is completed；Will
Black Semen Tritici aestivi after stirring imports conveyer belt, into automatic quantitative packing device, with half bag of sterilizing bag quantitative package, with the collar and breathing
Plug sealing, swings to sterilize in go-cart, per layer 6 bag, totally 9 layers；High temperature sterilize：After sterilizing go-cart is filled, bacteria district sterilizing cabinet is opened
Door, pushes sterilizing cabinet, and order is arranged, sterilizing cabinet closure door, evacuation, logical steam sterilization, 120 DEG C of sterilizing parameter, 0.10MPa, 2h,
Sterilizing is completed, aerofluxuss blood pressure lowering, treats that pressure, to zero, in clean room side hundred grades of purifying area sterilization cabinet doors is opened, and pulls out sterilizing go-cart,
Hundred grades of purification cooling zone cooling 10h make material temperature reach 18 DEG C；Aseptic inoculation：Compost after cooling is delivered to ten grades of purifications
Area, inoculation personnel open sterilizing bag breather plug in operating board, connect bacterium amount by 1% and access Agaricus Bisporus strain solid original seed or Agaricus Bisporus
Strain liquid original seed shakes up to sterilizing bag, and two sterilizing bags are poured in a culture bag, culture bag band respiratory membrane, is sealed with sealing machine
Mouthful, spread out of culturing room and cultivate；Constant temperature culture：Ten thousand grades purification culture room temperatures setting 24 DEG C of constant temperature, cultivate 15 days, strain into
It is ripe, it is sent to 2 DEG C of refrigeration house storages of constant temperature.
Embodiment three：Suitable for the Agaricus Bisporus strain mating system in standard chemical plant
High-quality is selected, the Agaricus Bisporus strain for flushing makees parent species selection-breeding, and does fruiting experiment, selects cultivating rate height, mushroom
Of fine quality, production capacity is in 30Kg/m2Bacterial strain above is used as Agaricus Bisporus strain parent species；Agaricus Bisporus strain parent species are adopted bacterium is cooked with Semen setariae
Silk carrier makes Agaricus Bisporus strain solid original seed Jing after breading protospecies or configuration nutritional solution is trained in liquid spawn fermentation tank Jing original seeds
Agaricus Bisporus strain liquid original seed is made after educating；Black Semen Tritici aestivi is screened, grain, flat grain and impurity is removed brokenly, is selected clean, satisfied
It is full, it is completely, fresh, without the black Semen Tritici aestivi for going mouldy；Foam material pond discharges water, and dissolving quick lime makes pH value reach 13.0, mix homogeneously, foam material
Cage is placed in foam material pond, and in foam material cage black Semen Tritici aestivi is put into, and is stirred with compressed air, and 6 times a day, makes black Semen Tritici aestivi water suction uniform；
Test black Semen Tritici aestivi water content reaches standard, and with hanging foam material cage is proposed, control water adds 1% to agitator without obvious water droplet, is poured into
The calcium sulfate of the Calcium Carbonate of mass fraction and 0.8% mass fraction, stirs, and makes pH value be adjusted to 9.0, and stirring is completed；Will
Black Semen Tritici aestivi after stirring imports conveyer belt, into automatic quantitative packing device, with half bag of sterilizing bag quantitative package, with the collar and breathing
Plug sealing, swings to sterilize in go-cart, per layer 6 bag, totally 9 layers；High temperature sterilize：After sterilizing go-cart is filled, bacteria district sterilizing cabinet is opened
Door, pushes sterilizing cabinet, and order is arranged, sterilizing cabinet closure door, evacuation, logical steam sterilization, 130 DEG C of sterilizing parameter, 0.20MPa, 3h,
Sterilizing is completed, aerofluxuss blood pressure lowering, treats that pressure, to zero, in clean room side hundred grades of purifying area sterilization cabinet doors is opened, and pulls out sterilizing go-cart,
Hundred grades of purification cooling zone cooling 14h make material temperature reach 25 DEG C；Aseptic inoculation：Compost after cooling is delivered to ten grades of purifications
Area, inoculation personnel open sterilizing bag breather plug in operating board, connect bacterium amount by 1% and access Agaricus Bisporus strain solid original seed or Agaricus Bisporus
Strain liquid original seed shakes up to sterilizing bag, and two sterilizing bags are poured in a culture bag, culture bag band respiratory membrane, is sealed with sealing machine
Mouthful, spread out of culturing room and cultivate；Constant temperature culture：Ten thousand grades purification culture room temperatures setting 24 DEG C of constant temperature, cultivate 18 days, strain into
It is ripe, it is sent to 2 DEG C of refrigeration house storages of constant temperature.
Example IV：Suitable for the Agaricus Bisporus strain mating system in standard chemical plant
High-quality is selected, the Agaricus Bisporus strain for flushing makees parent species selection-breeding, and does fruiting experiment, selects cultivating rate height, mushroom
Of fine quality, production capacity is in 30Kg/m2Bacterial strain above is used as Agaricus Bisporus strain parent species；Agaricus Bisporus strain parent species are adopted bacterium is cooked with Semen setariae
Silk carrier makes Agaricus Bisporus strain solid original seed Jing after breading protospecies or configuration nutritional solution is trained in liquid spawn fermentation tank Jing original seeds
Agaricus Bisporus strain liquid original seed is made after educating；Black Semen Tritici aestivi is screened, grain, flat grain and impurity is removed brokenly, is selected clean, satisfied
It is full, it is completely, fresh, without the black Semen Tritici aestivi for going mouldy；Foam material pond discharges water, and dissolving quick lime makes pH value reach 12.0, mix homogeneously, foam material
Cage is placed in foam material pond, and in foam material cage black Semen Tritici aestivi is put into, and is stirred with compressed air, and 5 times a day, makes black Semen Tritici aestivi water suction uniform；
Test black Semen Tritici aestivi water content reaches standard, and with hanging foam material cage is proposed, control water adds 1% to agitator without obvious water droplet, is poured into
The calcium sulfate of the Calcium Carbonate of mass fraction and 0.8% mass fraction, stirs, and makes pH value be adjusted to 8.5, and stirring is completed；Will
Black Semen Tritici aestivi after stirring imports conveyer belt, into automatic quantitative packing device, with half bag of sterilizing bag quantitative package, with the collar and breathing
Plug sealing, swings to sterilize in go-cart, per layer 6 bag, totally 9 layers；High temperature sterilize：After sterilizing go-cart is filled, bacteria district sterilizing cabinet is opened
Door, pushes sterilizing cabinet, and order is arranged, sterilizing cabinet closure door, evacuation, logical steam sterilization, 129 DEG C of sterilizing parameter, 0.18MPa, 2h,
Sterilizing is completed, aerofluxuss blood pressure lowering, treats that pressure, to zero, in clean room side hundred grades of purifying area sterilization cabinet doors is opened, and pulls out sterilizing go-cart,
Hundred grades of purification cooling zone cooling 11h make material temperature reach 22 DEG C；Aseptic inoculation：Compost after cooling is delivered to ten grades of purifications
Area, inoculation personnel open sterilizing bag breather plug in operating board, connect bacterium amount by 1% and access Agaricus Bisporus strain solid original seed or Agaricus Bisporus
Strain liquid original seed shakes up to sterilizing bag, and two sterilizing bags are poured in a culture bag, culture bag band respiratory membrane, is sealed with sealing machine
Mouthful, spread out of culturing room and cultivate；Constant temperature culture：Ten thousand grades purification culture room temperatures setting 24 DEG C of constant temperature, cultivate 16 days, strain into
It is ripe, it is sent to 2 DEG C of refrigeration house storages of constant temperature.
Embodiment five：Suitable for the Agaricus Bisporus strain mating system in standard chemical plant
High-quality is selected, the Agaricus Bisporus strain for flushing makees parent species selection-breeding, and does fruiting experiment, selects cultivating rate height, mushroom
Of fine quality, production capacity is in 30Kg/m2Bacterial strain above is used as Agaricus Bisporus strain parent species；Agaricus Bisporus strain parent species are adopted bacterium is cooked with Semen setariae
Silk carrier makes Agaricus Bisporus strain solid original seed Jing after breading protospecies or configuration nutritional solution is trained in liquid spawn fermentation tank Jing original seeds
Agaricus Bisporus strain liquid original seed is made after educating；Black Semen Tritici aestivi is screened, grain, flat grain and impurity is removed brokenly, is selected clean, satisfied
It is full, it is completely, fresh, without the black Semen Tritici aestivi for going mouldy；Foam material pond discharges water, and dissolving quick lime makes pH value reach 11.5, mix homogeneously, foam material
Cage is placed in foam material pond, and in foam material cage black Semen Tritici aestivi is put into, and is stirred with compressed air, and 6 times a day, makes black Semen Tritici aestivi water suction uniform；
Test black Semen Tritici aestivi water content reaches standard, and with hanging foam material cage is proposed, control water adds 1% to agitator without obvious water droplet, is poured into
The calcium sulfate of the Calcium Carbonate of mass fraction and 0.8% mass fraction, stirs, and makes pH value be adjusted to 8.0, and stirring is completed；Will
Black Semen Tritici aestivi after stirring imports conveyer belt, into automatic quantitative packing device, with half bag of sterilizing bag quantitative package, with the collar and breathing
Plug sealing, swings to sterilize in go-cart, per layer 6 bag, totally 9 layers；High temperature sterilize：After sterilizing go-cart is filled, bacteria district sterilizing cabinet is opened
Door, pushes sterilizing cabinet, and order is arranged, sterilizing cabinet closure door, evacuation, logical steam sterilization, 123 DEG C of sterilizing parameter, 0.15MPa,
2.5h, sterilizing is completed, aerofluxuss blood pressure lowering, treats that pressure, to zero, in clean room side hundred grades of purifying area sterilization cabinet doors is opened, and is pulled out sterilizing and is pushed away
Car, cooling zone cooling 12h is purified at hundred grades makes material temperature reach 24 DEG C；Aseptic inoculation：Compost after cooling is delivered to ten grades
Purifying area, inoculation personnel open sterilizing bag breather plug in operating board, connect bacterium amount by 1% and access Agaricus Bisporus strain solid original seed or double
Spore mushroom strains liquid original seed shakes up to sterilizing bag, and two sterilizing bags are poured in a culture bag, culture bag band respiratory membrane, with sealing
Machine is sealed, and is spread out of culturing room and is cultivated；Constant temperature culture：Ten thousand grades of purification culture room temperature 24 DEG C of constant temperature of setting, cultivate 18 days, strain
Maturation, is sent to 2 DEG C of refrigeration house storages of constant temperature.
Embodiment six：Suitable for the Agaricus Bisporus strain mating system in standard chemical plant
High-quality is selected, the Agaricus Bisporus strain for flushing makees parent species selection-breeding, and does fruiting experiment, selects cultivating rate height, mushroom
Of fine quality, production capacity is in 30Kg/m2Bacterial strain above is used as Agaricus Bisporus strain parent species；Agaricus Bisporus strain parent species are adopted bacterium is cooked with Semen setariae
Silk carrier makes Agaricus Bisporus strain solid original seed Jing after breading protospecies or configuration nutritional solution is trained in liquid spawn fermentation tank Jing original seeds
Agaricus Bisporus strain liquid original seed is made after educating；Black Semen Tritici aestivi is screened, grain, flat grain and impurity is removed brokenly, is selected clean, satisfied
It is full, it is completely, fresh, without the black Semen Tritici aestivi for going mouldy；Foam material pond discharges water, and dissolving quick lime makes pH value reach 12.5, mix homogeneously, foam material
Cage is placed in foam material pond, and in foam material cage black Semen Tritici aestivi is put into, and is stirred with compressed air, and 4 times a day, makes black Semen Tritici aestivi water suction uniform；
Test black Semen Tritici aestivi water content reaches standard, and with hanging foam material cage is proposed, control water adds 1% to agitator without obvious water droplet, is poured into
The calcium sulfate of the Calcium Carbonate of mass fraction and 0.8% mass fraction, stirs, and makes pH value be adjusted to 7.5, and stirring is completed；Will
Black Semen Tritici aestivi after stirring imports conveyer belt, into automatic quantitative packing device, with half bag of sterilizing bag quantitative package, with the collar and breathing
Plug sealing, swings to sterilize in go-cart, per layer 6 bag, totally 9 layers；High temperature sterilize：After sterilizing go-cart is filled, bacteria district sterilizing cabinet is opened
Door, pushes sterilizing cabinet, and order is arranged, sterilizing cabinet closure door, evacuation, logical steam sterilization, 125 DEG C of sterilizing parameter, 0.12MPa, 3h,
Sterilizing is completed, aerofluxuss blood pressure lowering, treats that pressure, to zero, in clean room side hundred grades of purifying area sterilization cabinet doors is opened, and pulls out sterilizing go-cart,
Hundred grades of purification cooling zone cooling 13h make material temperature reach 20 DEG C；Aseptic inoculation：Compost after cooling is delivered to ten grades of purifications
Area, inoculation personnel open sterilizing bag breather plug in operating board, connect bacterium amount by 1% and access Agaricus Bisporus strain solid original seed or Agaricus Bisporus
Strain liquid original seed shakes up to sterilizing bag, and two sterilizing bags are poured in a culture bag, culture bag band respiratory membrane, is sealed with sealing machine
Mouthful, spread out of culturing room and cultivate；Constant temperature culture：Ten thousand grades purification culture room temperatures setting 24 DEG C of constant temperature, cultivate 17 days, strain into
It is ripe, it is sent to 2 DEG C of refrigeration house storages of constant temperature.
Embodiment seven：Suitable for the process optimization of the Agaricus Bisporus strain mating system in standard chemical plant
Under the performance of above-mentioned series embodiment, it is further provided the Agaricus Bisporus strain mating system of optimization, respectively
Button mushroom is bred using following different technological parameter：
Scheme one：Fermentation vat pH value 11.0, compressed air is stirred daily 4 times, raw material stirring pH value 7.0, sterilising temp 120
DEG C, sterilization pressure 0.10MPa, sterilization time 2h, cool time 10h, 18 DEG C of material temperature, incubation time 15 days.
Scheme two：Fermentation vat pH value 13.0, compressed air is stirred daily 6 times, raw material stirring pH value 9.0, sterilising temp 130
DEG C, sterilization pressure 0.20MPa, sterilization time 3h, cool time 14h, 25 DEG C of material temperature, incubation time 18 days.
Scheme three：Fermentation vat pH value 12.0, compressed air is stirred daily 5 times, raw material stirring pH value 8.5, sterilising temp 129
DEG C, sterilization pressure 0.18MPa, sterilization time 2h, cool time 11h, 22 DEG C of material temperature, incubation time 16 days.
Scheme four：Fermentation vat pH value 11.5, compressed air is stirred daily 6 times, raw material stirring pH value 8.0, sterilising temp 123
DEG C, sterilization pressure 0.15MPa, sterilization time 2.5h, cool time 12h, 24 DEG C of material temperature, incubation time 18 days.
Scheme five：Fermentation vat pH value 12.5, compressed air is stirred daily 4 times, raw material stirring pH value 7.5, sterilising temp 125
DEG C, sterilization pressure 0.12MPa, sterilization time 3h, cool time 13h, 20 DEG C of material temperature, incubation time 17 days.
The processing method that five different schemes of above-mentioned offer are provided respectively according to embodiment one is bred into button mushroom：
1. orthogonal optimization button mushroom breeds technique
Design experiment of single factor, probe into respectively button mushroom breed in technique fermentation vat pH value A, raw material stirring pH value B and
Impact of the material temperature to button mushroom qualification rate.Three factors-levels orthogonal experiment is carried out on the basis of experiment of single factor, it is orthogonal
Experimental factor is shown in Table 1 with level.
Table 1：Orthogonal test factor and water-glass
Orthogonal optimization button mushroom breeds engineer testing result and analysis in table 2.
Table 2：Orthogonal experiments and analysis
By the extreme difference of relatively more each index, each influence factor's primary and secondary order is B>C>A, i.e. button mushroom breed raw materials technology
Stirring pH value is major influence factors, next to that material temperature and fermentation vat pH value, according to k1, k2, k3 value of each index in table 2 and is become
The result of gesture figure, determines that each factor optimum level is combined as A2B2C3, i.e., the optimize technique in wheat flour manufacturing process is：Immersion
Pond pH value 11.0-13.0, raw material stirring pH value 7.0-9.0,18 DEG C -25 DEG C of material temperature；Preferably, fermentation vat pH value 12.0, raw material
Stirring pH value 8.0,25 DEG C of material temperature.
2. response phase method optimization button mushroom breeds technique, and experimental factor is shown in Table 3 with level：
Table 3：Response surface experiments factor and water-glass
Table 4：Response surface experiments are designed and result
| Numbering | A sterilising temps/DEG C | B sterilization pressures/Mpa | C sterilization times/h | Qualification rate/% |
| 1 | 125 | 0.2 | 2.0 | 78 |
| 2 | 130 | 0.2 | 2.5 | 82 |
| 3 | 120 | 0.15 | 3.0 | 76 |
| 4 | 125 | 0.2 | 3.0 | 78 |
| 5 | 120 | 0.2 | 2.5 | 84 |
| 6 | 125 | 0.15 | 2.5 | 96 |
| 7 | 130 | 0.15 | 3.0 | 83 |
| 8 | 120 | 0.15 | 2.0 | 76 |
| 9 | 125 | 0.15 | 2.5 | 94 |
| 10 | 120 | 0.1 | 2.5 | 75 |
| 11 | 125 | 0.1 | 2.0 | 79 |
| 12 | 125 | 0.15 | 2.5 | 97 |
| 13 | 125 | 0.15 | 2.5 | 96 |
| 14 | 125 | 0.15 | 2.5 | 95 |
| 15 | 130 | 0.1 | 2.5 | 78 |
| 16 | 130 | 0.15 | 2.0 | 82 |
| 17 | 125 | 0.1 | 3.0 | 79 |
Table 5：Response surface experiments analysis of variance table
Note：* represent notable, * * represent extremely notable.
Response surface experiments are designed and result：
Design of experiment analysis is carried out according to Box-Benhnken center combination designs principle, it the results are shown in Table 4,5.Pass through
Design Expert8.0.6 are fitted using the method for polynomial fitting to 4,5 experimental data, obtain qualification rate R to sterilizing
The secondary multinomial regression model of temperature A, sterilization pressure B and sterilization time C is：
R=+95.8+1.75*A+1.38*B+0.13*C-1.25*A*B+0.25*A*C-7.65*
A^2-8.40*B^2-8.90*C^2
In response surface analysis each factor reciprocal action referring specifically to accompanying drawing 2 to accompanying drawing 4.From orthogonal test, response surface experiments
Optimization button mushroom is bred the result of technique and understands that the optimize technique that button mushroom is bred in technical process is：Sterilising temp 120
DEG C -130 DEG C, sterilization pressure 0.10MPa-0.20MPa, sterilization time 2h-3h；Preferably, 125 DEG C of sterilising temp, sterilization pressure
0.15MPa, sterilization time 2.5h.
A kind of Agaricus Bisporus strain mating system suitable for standard chemical plant provided using above-described embodiment one, Jing is orthogonal
Test and response phase method optimize processing technology, a kind of Agaricus Bisporus strain side of breeding suitable for standard chemical plant that the present invention is provided
Method Jing response phase method optimize technique, can in large quantity breed out that high-quality is pure, high yield Agaricus Bisporus cultigen, more traditional training
Educate method cultivation period and compared for 30-45 days and cultivation period is greatly shortened, while reducing pollution probability, cultigen is typically trained
The time is educated for -18 days 15 days, without steaming and decocting black Semen Tritici aestivi, energy saving, the strain grain water content cultivated is consistent, and long bacterium does not occur
By phenomenon, qualification rate, due to breeding three-level kind from two grades of kinds, no matter doing solid spawn with grain, still uses liquid up to 96%
Body strain, they are uniformly blended into or are sprayed in black Semen Tritici aestivi culture medium in all can purifying rank environment at ten grades, and growing point is more,
Sprout fast, it is unanimous on the whole with the inoculation time being subject on each granule of a collection of substrate, so the time point of initial growth is consistent,
Cell age is consistent, and cell age is short, and strain vitality is strong, and mycelia is evenly distributed specification, has wide applicability in strainsfor cultivation field.
As mentioned above, you can preferably realize the present invention, the above embodiments are only the side of being preferable to carry out to the present invention
Formula is described, and not the scope of the present invention is defined, and on the premise of without departing from design spirit of the present invention, this area is general
Various modifications and improvement that logical technical staff makes to technical scheme, all should fall into present invention determine that protection domain
It is interior.
Claims (2)
1. a kind of Agaricus Bisporus strain mating system suitable for standard chemical plant, specifically using following technical step：
（1）It is prepared by original seed：High-quality is selected, the Agaricus Bisporus strain for flushing makees parent species selection-breeding, and does fruiting experiment, selects fruiting
Rate height, good mushroom quality, production capacity are in 30Kg/m2Bacterial strain above is used as Agaricus Bisporus strain parent species；By Agaricus Bisporus strain parent species adopt with
Semen setariae is cooked mycelia carrier and Agaricus Bisporus strain solid original seed or configuration nutritional solution is made Jing after breading protospecies in liquid spawn fermentation tank
Agaricus Bisporus strain liquid original seed is made Jing after breading protospecies；
（2）Material choice：Black Semen Tritici aestivi is screened, grain, flat grain and impurity is removed brokenly, is selected clean, it is full, it is completely, fresh,
Without the black Semen Tritici aestivi for going mouldy；
（3）Raw material soaking：Foam material pond discharges water, and dissolving quick lime makes pH value reach 11.0-13.0, and mix homogeneously, foam material cage is put
In foam material pond, black Semen Tritici aestivi is put in foam material cage, is stirred with compressed air, 4 times a day -6 times, make black Semen Tritici aestivi water suction uniform；
（4）Raw material stirring：Test black Semen Tritici aestivi water content reaches standard, and with hanging foam material cage is proposed, control water to without obvious water droplet,
Enter agitator, add the Calcium Carbonate of 1% mass fraction and the calcium sulfate of 0.8% mass fraction, stir, be adjusted to pH value
7.0-9.0, stirring is completed；
（5）Quantitative package：Black Semen Tritici aestivi after stirring is imported into conveyer belt, into automatic quantitative packing device, is quantitatively wrapped with sterilizing bag
Half bag is filled, is sealed with the collar and breather plug, swing to sterilize in go-cart, per layer 6 bag, totally 9 layers；
（6）High temperature sterilize：After sterilizing go-cart is filled, bacteria district sterilization cabinet door is opened, push sterilizing cabinet, order is arranged, sterilizing cabinet
Closure door, evacuation, logical steam sterilization, 120 DEG C -130 DEG C of sterilizing parameter, 0.10MPa-0.20MPa, 2h-3h, sterilizing is completed, row
Gas blood pressure lowering, treats that pressure, to zero, in clean room side hundred grades of purifying area sterilization cabinet doors is opened, and pulls out sterilizing go-cart, cold in hundred grades of purifications
But cooling 10h-14h in area's makes material temperature reach 18 DEG C -25 DEG C；
（7）Aseptic inoculation：Compost after cooling is delivered to ten grades of purifying areas, inoculation personnel open sterilizing bag and exhale in operating board
Plug is inhaled, bacterium amount is connect by 1% and is accessed Agaricus Bisporus strain solid original seed or Agaricus Bisporus strain liquid original seed to sterilizing bag, shaken up, two sterilizings
Bag is poured in a culture bag, culture bag band respiratory membrane, is sealed with sealing machine, is spread out of culturing room and is cultivated；
（8）Constant temperature culture：Ten thousand grades of purification culture room temperature 24 DEG C of constant temperature of setting, cultivate -18 days 15 days, and strain is ripe, is sent to
2 DEG C of refrigeration house storages of constant temperature.
2. a kind of Agaricus Bisporus strain mating system suitable for standard chemical plant as claimed in claim 1, it is characterised in that institute
Foam material pond pH value is preferably 12.0 in the raw material soaking step stated, and raw material stirring pH value is preferably 8.0,21.5 DEG C of material temperature, high temperature
Sterilizing parameter is preferably 125 DEG C in sterilization steps, 0.15MPa, 2.5h.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107602197A (en) * | 2017-10-30 | 2018-01-19 | 山东瑞蕈天库菌种开发有限公司 | A kind of preparation method of the high-quality White mushroom Cultivar culture medium of industrialized production |
| CN108812063A (en) * | 2018-08-22 | 2018-11-16 | 福建省农业科学院食用菌研究所（福建省蘑菇菌种研究推广站） | A method of cultivation of agaricus bisporus kind is made using synthetic substrate |
| CN109832091A (en) * | 2019-01-20 | 2019-06-04 | 香格里拉市腊玛农产品开发有限公司 | A kind of cultural method of the golden ear of half bag of mulberry wood chips plantation |
| CN115191289A (en) * | 2021-04-08 | 2022-10-18 | 临沂瑞泽生物科技股份有限公司 | Edible mushroom culture process and equipment |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000065542A (en) * | 1999-04-06 | 2000-11-15 | 강석춘 | A process for preparing a yang-song-i mushroom using horse-droppings as its raw materials |
| KR20030059775A (en) * | 2003-05-24 | 2003-07-10 | 광미실업(주) | Mushroom medium composition, mushroom medium using the same, and method for cultivating mushroom |
| KR20120091920A (en) * | 2011-02-10 | 2012-08-20 | 주식회사 에코엔 | Culture medium composition for mushroom(agaricus bisporus) with high amino acids content and its preparation method, and a method of cultivating mushroom using it |
| CN103340090A (en) * | 2012-01-09 | 2013-10-09 | 福建省农业科学院土壤肥料研究所 | Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer |
| CN104823712A (en) * | 2015-04-30 | 2015-08-12 | 上海市农业科学院 | Anti-aging agaricus bisporus wheat strain and preparation method thereof |
| CN104920070A (en) * | 2015-06-17 | 2015-09-23 | 江苏省农业科学院宿迁农科所 | Method for increasing yield of agaricus bisporus |
| CN105453895A (en) * | 2015-12-23 | 2016-04-06 | 嘉兴圣洲生物科技有限公司 | Cultivation technology for Agaricus blazei |
| CN105567572A (en) * | 2016-01-04 | 2016-05-11 | 山东省科创食用菌产业技术研究院 | Preparation technology of agaricus bisporus strain |
-
2016
- 2016-12-30 CN CN201611264852.7A patent/CN106676018B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000065542A (en) * | 1999-04-06 | 2000-11-15 | 강석춘 | A process for preparing a yang-song-i mushroom using horse-droppings as its raw materials |
| KR20030059775A (en) * | 2003-05-24 | 2003-07-10 | 광미실업(주) | Mushroom medium composition, mushroom medium using the same, and method for cultivating mushroom |
| KR20120091920A (en) * | 2011-02-10 | 2012-08-20 | 주식회사 에코엔 | Culture medium composition for mushroom(agaricus bisporus) with high amino acids content and its preparation method, and a method of cultivating mushroom using it |
| CN103340090A (en) * | 2012-01-09 | 2013-10-09 | 福建省农业科学院土壤肥料研究所 | Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer |
| CN104823712A (en) * | 2015-04-30 | 2015-08-12 | 上海市农业科学院 | Anti-aging agaricus bisporus wheat strain and preparation method thereof |
| CN104920070A (en) * | 2015-06-17 | 2015-09-23 | 江苏省农业科学院宿迁农科所 | Method for increasing yield of agaricus bisporus |
| CN105453895A (en) * | 2015-12-23 | 2016-04-06 | 嘉兴圣洲生物科技有限公司 | Cultivation technology for Agaricus blazei |
| CN105567572A (en) * | 2016-01-04 | 2016-05-11 | 山东省科创食用菌产业技术研究院 | Preparation technology of agaricus bisporus strain |
Non-Patent Citations (2)
| Title |
|---|
| 刘刚: "《不同培养基和pH 值对双孢菇母种菌丝生长的影响》", 《现代农业科技》 * |
| 黄瑞贤等主编: "《黑木耳袋栽小口出耳新技术》", 28 February 2016 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107602197A (en) * | 2017-10-30 | 2018-01-19 | 山东瑞蕈天库菌种开发有限公司 | A kind of preparation method of the high-quality White mushroom Cultivar culture medium of industrialized production |
| CN108812063A (en) * | 2018-08-22 | 2018-11-16 | 福建省农业科学院食用菌研究所（福建省蘑菇菌种研究推广站） | A method of cultivation of agaricus bisporus kind is made using synthetic substrate |
| CN108812063B (en) * | 2018-08-22 | 2021-01-01 | 福建省农业科学院食用菌研究所（福建省蘑菇菌种研究推广站） | Method for preparing agaricus bisporus cultivated species by adopting synthetic matrix |
| CN109832091A (en) * | 2019-01-20 | 2019-06-04 | 香格里拉市腊玛农产品开发有限公司 | A kind of cultural method of the golden ear of half bag of mulberry wood chips plantation |
| CN115191289A (en) * | 2021-04-08 | 2022-10-18 | 临沂瑞泽生物科技股份有限公司 | Edible mushroom culture process and equipment |
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