CN104838883A - Toadstool strain preparation process - Google Patents
Toadstool strain preparation process Download PDFInfo
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- CN104838883A CN104838883A CN201510192817.8A CN201510192817A CN104838883A CN 104838883 A CN104838883 A CN 104838883A CN 201510192817 A CN201510192817 A CN 201510192817A CN 104838883 A CN104838883 A CN 104838883A
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Abstract
The invention discloses a toadstool strain preparation process. The process includes the following steps of preparing and cultivating mother strains, preparing and cultivating protospecies, and preparing and cultivating toadstool strains. The process is simple and easy to operate and low in cost, prepared strain mycelia are rapid in growth, sturdy and dense, and the survival rate and yield of toadstool breeding are increased.
Description
Technical field
The invention belongs to fungus growing technique field, relate to the preparation technology of a kind of edible mushroom, be specifically related to a kind of preparation technology of hickory chick bacterial classification.
Background technology
Hickory chick has another name called sheep tripe dish, is morchella edible mushroom, is a kind of edible mushroom and medicinal fungus of preciousness; The nutrition of hickory chick is quite abundant, and hickory chick is containing the polysaccharide of Tumor suppression, and antibacterial, antiviral active component, has all multiactions such as strengthening immunity of organisms, antifatigue, antiviral, Tumor suppression; Because hickory chick is a kind of very precious wild mushroom, and can sell on the market as food materials, therefore hickory chick needs are large-area carries out plant development, and keeps the good quality and high output of hickory chick to need excellent hickory chick bacterial classification.
Current hickory chick bacterial classification still adopts original breeding method, in medium, the blend proportion of each composition is unreasonable, cultivating process lacks effective control and management, composition, the particle size of different material are different with wood fibre physical arrangement, there is important impact in capital on edible fungus species quality, and researching and developing a kind of hickory chick bacterial classification turning out high-quality hickory chick becomes those skilled in the art's technical problem urgently to be resolved hurrily.
Summary of the invention
Technical problem to be solved by this invention is, for the shortcoming that above prior art exists, a kind of preparation technology of hickory chick bacterial classification is proposed, this bacterial classification preparation technology, operation is simple, and cost is low, and the bacterial classification mycelial growth prepared is fast, mycelia is sturdy dense, improves survival rate and the output of hickory chick plantation.
The technical scheme that the present invention solves above technical problem is:
A preparation technology for hickory chick bacterial classification, this preparation technology's thalline comprises the following steps:
(1) female making of planting and cultivation;
Mother culture media is by the potato of 50-60 part according to the mass fraction, the agar of 8-14 part, the glucose composition of Cobastab agent 5-7 part of 0.5-0.8 part, the water that 1500ml all put into by potato, agar, Cobastab and glucose is heated, until the agar be added to the water all boiling namely to obtain mother culture media stand-by;
The mother culture media 150ml produced is got with the test tube of 250ml, test tube openend is clogged with cotton, test tube is placed on sterilizing 1-2h in the high-pressure sterilizing pot at 115-130 DEG C, then room temperature is naturally cooled to, the aseptic inoculation box putting into sealing will be tried, access Morciiella Esculeuta Mycelia in each test tube, be placed in 20-23 DEG C of incubator and cultivate, until grow into female kind;
(2) making of original seed and cultivation;
The composts or fertilisers of cultivating of original seed comprises following component, corn according to the mass fraction: 60-75 part, wheat: 50-60 part, paddy: 15-20 part;
The fabrication processing of the composts or fertilisers of cultivating of original seed is: weigh-soak-boil and expect-drain-packing-sealing-sterilizing-steriling test-inoculation-cultivation, wherein:
Weigh, take corn, wheat, the paddy selected respectively;
Soak, corn, wheat, paddy are soaked respectively, corn soaking 30-34h, and change a water every 5h and prevent particle souring, wheat soaks 3-4h, and paddy soaks 1-3h;
Boil material, soaked corn, wheat, paddy are placed in pot respectively and heat poach, until the seed coat of corn, wheat, paddy just breaks, then pull out, drain unnecessary moisture;
Packing, the corn after draining, wheat, paddy are loaded in the wide-mouth bottle of 100ml, it is 35-40g that every bottle of charge weight amounts to dry weight;
Sealing, point polypropylene duplicature of the wide-mouth bottle 10cm × 10cm installed is sealed a bottle hole, and cutting off a diameter at polypropylene duplicature facing to wide-mouth bottle position of bottleneck is the hole of 1.5cm, and seals with two-layer brown paper, with the fixing sealing of cotton rope;
Sterilizing, by the wide-mouth bottle sterilizing 20-25h at 120-130 DEG C after sealing, then adopts chilled air cools;
Steriling test, is placed in insulating box by the composts or fertilisers of cultivating after sterilizing and cultivates 1-3 days, if asepsis growth, sterilizing thoroughly can use; If there is bacteria growing, sterilizing is not thorough, from Amoxcillin until steriling test success;
Inoculation, aseptically, planting the mother prepared by every bottle of inoculum concentration is that 2-4g is inoculated in the test tube of composts or fertilisers of cultivating, constant temperature culture at the bacterial classification connected is placed in 23-25 DEG C;
(3) making of hickory chick bacterial classification and cultivation;
Select corncob according to the mass fraction: 75-80 part, wheat particle: 10-15 part, wood chip: 10-15 part, superphosphate: 1-3 part, urea: 1-2 part, as the composts or fertilisers of cultivating of hickory chick bacterial classification, stirs the corncob in composts or fertilisers of cultivating, wheat particle, wood chip, superphosphate, urea, maintenance water content is 75-80%, then loads in Polypropylene Bag and seals;
The Polypropylene Bag of sealing is put into autoclave sterilizing, preserves 1-3h for 100-108 DEG C under normal pressure, take out and be cooled to room temperature;
Polypropylene Bag and Primary spawn material that hickory chick strain culture-material is housed are placed in inoculating hood in the lump, and seal 0.5-1h, original seed is linked in the Polypropylene Bag that hickory chick strain culture-material is housed, and send into culturing room, the temperature controlling culturing room is 20-23 DEG C, cultivates and obtains hickory chick cultivation strain in 13-15 days.
The technical scheme that the present invention limits further is:
In the preparation technology of aforementioned hickory chick bacterial classification, the corn in Primary spawn material, wheat, paddy all select full grains, free from insect pests, the corn without going mouldy, wheat, paddy dedusting is for subsequent use.
In the preparation technology of aforementioned hickory chick bacterial classification, the preparation method of the Morciiella Esculeuta Mycelia that step (1) accesses in test tube when the making that mother plants is specially: your fruit body of fresh hickory chick of gathering, and fruit body is cut into the bacterium block of 1-2mm, allow and bacterium block is positioned on PDA medium, PDA medium is placed in the incubator of 20-23 DEG C, after 5-8 days, namely obtains Morciiella Esculeuta Mycelia.
The invention has the beneficial effects as follows:
When sealing, seal a bottle hole with polypropylene duplicature, cutting off a diameter at polypropylene duplicature facing to wide-mouth bottle position of bottleneck is the hole of 1.5cm, and seal with two-layer brown paper, with the fixing sealing of cotton rope, be so both beneficial to ventilation moisturizing, when being convenient to again inoculation, reduce pollution rate.
In the present invention, original seed composts or fertilisers of cultivating re-uses after all needing particle to boil brokenly, be conducive to Morciiella Esculeuta Mycelia growth like this, because morchella is in sac fungi, and all sac fungis are all monokaryon bacterium, mycelia is trickleer than double-core bacterium, weak to the decomposition Utilization ability of nutriment, after boiling brokenly, starch can discharge from the particle boiled brokenly, is conducive to Morchella esculenta (L.) Pers mycelium and absorbs, and makes hickory chick to the abundant absorption of composts or fertilisers of cultivating nutrition.
In the preparation process of hickory chick bacterial classification, by improving culture medium prescription of the prior art, at the way to cultivate that each stage of breeding strain adopts different medium to reach best, the process for preparing strain thereof that this programme provides, the hickory chick bacterial classification prepared is best in quality, meanwhile, the survival rate of hickory chick plantation is substantially increased.
The present invention selects materials when the making of original seed, soak, boil material, the link of sterilizing and inoculation carries out strict control, also be the key of production of hybrid seeds success or failure, the factors such as soak time, volume, temperature, water temperature of each component in strict control Primary spawn material, make to do kind of the hickory chick bacterial classification survival rate prepared better, output is high.
Embodiment
embodiment 1
The present embodiment provides a kind of preparation technology of hickory chick bacterial classification, and this preparation technology's thalline comprises the following steps:
(1) female making of planting and cultivation;
Mother culture media is by the potato of 50 parts according to the mass fraction, the agar of 14 parts, the glucose composition of the Cobastab agent 6 parts of 0.5 part, the water that 1500ml all put into by potato, agar, Cobastab and glucose is heated, until the agar be added to the water all boiling namely to obtain mother culture media stand-by;
The mother culture media 150ml produced is got with the test tube of 250ml, test tube openend is clogged with cotton, test tube is placed on sterilizing 2h in the high-pressure sterilizing pot at 115 DEG C, then room temperature is naturally cooled to, the aseptic inoculation box putting into sealing will be tried, access Morciiella Esculeuta Mycelia in each test tube, be placed in 22 DEG C of incubators and cultivate, until grow into female kind;
Female plant making time test tube in the preparation method of Morciiella Esculeuta Mycelia that accesses be specially: your fruit body of fresh hickory chick of gathering, and fruit body is cut into the bacterium block of 1mm, allow and bacterium block is positioned on PDA medium, PDA medium is placed in the incubator of 20 DEG C, after 5 days, namely obtains Morciiella Esculeuta Mycelia;
(2) making of original seed and cultivation;
The composts or fertilisers of cultivating of original seed comprises following component, corn according to the mass fraction: 75 parts, wheat: 50 parts, paddy: 18 parts; Corn, wheat, paddy all select full grains, free from insect pests, the corn without going mouldy, wheat, paddy dedusting is for subsequent use;
The fabrication processing of the composts or fertilisers of cultivating of original seed is: weigh-soak-boil and expect-drain-packing-sealing-sterilizing-steriling test-inoculation-cultivation, wherein:
Weigh, take corn, wheat, the paddy selected respectively;
Soak, corn, wheat, paddy are soaked respectively, corn soaking 30h, and change a water every 5h, wheat soaks 3h, and paddy soaks 1h;
Boil material, soaked corn, wheat, paddy are placed in pot respectively and heat poach, until the seed coat of corn, wheat, paddy just breaks, then pull out, drain unnecessary moisture;
Packing, the corn after draining, wheat, paddy are loaded in the wide-mouth bottle of 100ml, it is 35g that every bottle of charge weight amounts to dry weight;
Sealing, point polypropylene duplicature of the wide-mouth bottle 10cm × 10cm installed is sealed a bottle hole, and cutting off a diameter at polypropylene duplicature facing to wide-mouth bottle position of bottleneck is the hole of 1.5cm, and seals with two-layer brown paper, with the fixing sealing of cotton rope;
Sterilizing, by the wide-mouth bottle sterilizing 22h at 121 DEG C after sealing, then adopts chilled air cools;
Steriling test, the composts or fertilisers of cultivating after sterilizing is placed in insulating box and cultivates 2 days, if asepsis growth, sterilizing thoroughly can use; If there is bacteria growing, sterilizing is not thorough, from Amoxcillin until steriling test success;
Inoculation, aseptically, planting the mother prepared by every bottle of inoculum concentration is that 2g is inoculated in the test tube of composts or fertilisers of cultivating, constant temperature culture at the bacterial classification connected is placed in 25 DEG C;
(3) making of hickory chick bacterial classification and cultivation;
Select corncob according to the mass fraction: 80 parts, wheat particle: 13 parts, wood chip: 10 parts, superphosphate: 1 part, urea: 1 part, as the composts or fertilisers of cultivating of hickory chick bacterial classification, stirs the corncob in composts or fertilisers of cultivating, wheat particle, wood chip, superphosphate, urea, keep water content to be 80%, then load in Polypropylene Bag and seal;
The Polypropylene Bag of sealing is put into autoclave sterilizing, and lower 108 DEG C of normal pressure preserves 2h, takes out and is cooled to room temperature;
Polypropylene Bag and Primary spawn material that hickory chick strain culture-material is housed are placed in inoculating hood in the lump, and seal 0.5h, original seed is linked in the Polypropylene Bag that hickory chick strain culture-material is housed, and send into culturing room, the temperature controlling culturing room is 20 DEG C, cultivates and obtains hickory chick cultivation strain in 14 days.
embodiment 2
The present embodiment provides a kind of preparation technology of hickory chick bacterial classification, and this preparation technology's thalline comprises the following steps:
(1) female making of planting and cultivation;
Mother culture media is by the potato of 60 parts according to the mass fraction, the agar of 11 parts, the glucose composition of the Cobastab agent 5 parts of 0.7 part, the water that 1500ml all put into by potato, agar, Cobastab and glucose is heated, until the agar be added to the water all boiling namely to obtain mother culture media stand-by;
The mother culture media 150ml produced is got with the test tube of 250ml, test tube openend is clogged with cotton, test tube is placed on sterilizing 1h in the high-pressure sterilizing pot at 121 DEG C, then room temperature is naturally cooled to, the aseptic inoculation box putting into sealing will be tried, access Morciiella Esculeuta Mycelia in each test tube, be placed in 23 DEG C of incubators and cultivate, until grow into female kind;
Female plant making time test tube in the preparation method of Morciiella Esculeuta Mycelia that accesses be specially: your fruit body of fresh hickory chick of gathering, and fruit body is cut into the bacterium block of 2mm, allow and bacterium block is positioned on PDA medium, PDA medium is placed in the incubator of 23 DEG C, after 8 days, namely obtains Morciiella Esculeuta Mycelia;
(2) making of original seed and cultivation;
The composts or fertilisers of cultivating of original seed comprises following component, corn according to the mass fraction: 60 parts, wheat: 55 parts, paddy: 20 parts; Corn, wheat, paddy all select full grains, free from insect pests, the corn without going mouldy, wheat, paddy dedusting is for subsequent use;
The fabrication processing of the composts or fertilisers of cultivating of original seed is: weigh-soak-boil and expect-drain-packing-sealing-sterilizing-steriling test-inoculation-cultivation, wherein:
Weigh, take corn, wheat, the paddy selected respectively;
Soak, corn, wheat, paddy are soaked respectively, corn soaking 34h, and change a water every 5h, wheat soaks 4h, and paddy soaks 2h;
Boil material, soaked corn, wheat, paddy are placed in pot respectively and heat poach, until the seed coat of corn, wheat, paddy just breaks, then pull out, drain unnecessary moisture;
Packing, the corn after draining, wheat, paddy are loaded in the wide-mouth bottle of 100ml, it is 40g that every bottle of charge weight amounts to dry weight;
Sealing, point polypropylene duplicature of the wide-mouth bottle 10cm × 10cm installed is sealed a bottle hole, and cutting off a diameter at polypropylene duplicature facing to wide-mouth bottle position of bottleneck is the hole of 1.5cm, and seals with two-layer brown paper, with the fixing sealing of cotton rope;
Sterilizing, by the wide-mouth bottle sterilizing 20h at 120 DEG C after sealing, then adopts chilled air cools;
Steriling test, the composts or fertilisers of cultivating after sterilizing is placed in insulating box and cultivates 1 day, if asepsis growth, sterilizing thoroughly can use; If there is bacteria growing, sterilizing is not thorough, from Amoxcillin until steriling test success;
Inoculation, aseptically, planting the mother prepared by every bottle of inoculum concentration is that 3g is inoculated in the test tube of composts or fertilisers of cultivating, constant temperature culture at the bacterial classification connected is placed in 23 DEG C;
(3) making of hickory chick bacterial classification and cultivation;
Select corncob according to the mass fraction: 78 parts, wheat particle: 15 parts, wood chip: 15 parts, superphosphate: 2 parts, urea: 2 parts, as the composts or fertilisers of cultivating of hickory chick bacterial classification, stirs the corncob in composts or fertilisers of cultivating, wheat particle, wood chip, superphosphate, urea, keep water content 75%, then load in Polypropylene Bag and seal;
The Polypropylene Bag of sealing is put into autoclave sterilizing, and lower 100 DEG C of normal pressure preserves 1h, takes out and is cooled to room temperature;
Polypropylene Bag and Primary spawn material that hickory chick strain culture-material is housed are placed in inoculating hood in the lump, and seal 1h, original seed is linked in the Polypropylene Bag that hickory chick strain culture-material is housed, and sends into culturing room, the temperature controlling culturing room is 23 DEG C, cultivates and obtains hickory chick cultivation strain in 15 days.
embodiment 3
The present embodiment provides a kind of preparation technology of hickory chick bacterial classification, and this preparation technology's thalline comprises the following steps:
(1) female making of planting and cultivation;
Mother culture media is by the potato of 55 parts according to the mass fraction, the agar of 8 parts, the glucose composition of the Cobastab agent 7 parts of 0.8 part, heats the water that 1500ml all put into by potato, agar, Cobastab and glucose, until the agar be added to the water all boiling namely to obtain mother culture media stand-by;
The mother culture media 150ml produced is got with the test tube of 250ml, test tube openend is clogged with cotton, test tube is placed on sterilizing 1h in the high-pressure sterilizing pot at 130 DEG C, then room temperature is naturally cooled to, the aseptic inoculation box putting into sealing will be tried, access Morciiella Esculeuta Mycelia in each test tube, be placed in 20 DEG C of incubators and cultivate, until grow into female kind;
Female plant making time test tube in the preparation method of Morciiella Esculeuta Mycelia that accesses be specially: your fruit body of fresh hickory chick of gathering, and fruit body is cut into the bacterium block of 2mm, allow and bacterium block is positioned on PDA medium, PDA medium is placed in the incubator of 21 DEG C, after 7 days, namely obtains Morciiella Esculeuta Mycelia;
(2) making of original seed and cultivation;
The composts or fertilisers of cultivating of original seed comprises following component, corn according to the mass fraction: 60 parts, wheat: 60 parts, paddy: 15 parts; Corn, wheat, paddy all select full grains, free from insect pests, the corn without going mouldy, wheat, paddy dedusting is for subsequent use;
The fabrication processing of the composts or fertilisers of cultivating of original seed is: weigh-soak-boil and expect-drain-packing-sealing-sterilizing-steriling test-inoculation-cultivation, wherein:
Weigh, take corn, wheat, the paddy selected respectively;
Soak, corn, wheat, paddy are soaked respectively, corn soaking 32h, and change a water every 5h, wheat soaks 4h, and paddy soaks 3h;
Boil material, soaked corn, wheat, paddy are placed in pot respectively and heat poach, until the seed coat of corn, wheat, paddy just breaks, then pull out, drain unnecessary moisture;
Packing, the corn after draining, wheat, paddy are loaded in the wide-mouth bottle of 100ml, it is 38g that every bottle of charge weight amounts to dry weight;
Sealing, point polypropylene duplicature of the wide-mouth bottle 10cm × 10cm installed is sealed a bottle hole, and cutting off a diameter at polypropylene duplicature facing to wide-mouth bottle position of bottleneck is the hole of 1.5cm, and seals with two-layer brown paper, with the fixing sealing of cotton rope;
Sterilizing, by the wide-mouth bottle sterilizing 25h at 130 DEG C after sealing, then adopts chilled air cools;
Steriling test, the composts or fertilisers of cultivating after sterilizing is placed in insulating box and cultivates 3 days, if asepsis growth, sterilizing thoroughly can use; If there is bacteria growing, sterilizing is not thorough, from Amoxcillin until steriling test success;
Inoculation, aseptically, planting the mother prepared by every bottle of inoculum concentration is that 4g is inoculated in the test tube of composts or fertilisers of cultivating, constant temperature culture at the bacterial classification connected is placed in 24 DEG C;
(3) making of hickory chick bacterial classification and cultivation;
Select corncob according to the mass fraction: 78 parts, wheat particle: 10 parts, wood chip: 12 parts, superphosphate: 3 parts, urea: 2 parts, as the composts or fertilisers of cultivating of hickory chick bacterial classification, stirs the corncob in composts or fertilisers of cultivating, wheat particle, wood chip, superphosphate, urea, keep water content to be 78%, then load in Polypropylene Bag and seal;
The Polypropylene Bag of sealing is put into autoclave sterilizing, and lower 105 DEG C of normal pressure preserves 3h, takes out and is cooled to room temperature;
Polypropylene Bag and Primary spawn material that hickory chick strain culture-material is housed are placed in inoculating hood in the lump, and seal 0.8h, original seed is linked in the Polypropylene Bag that hickory chick strain culture-material is housed, and send into culturing room, the temperature controlling culturing room is 21 DEG C, cultivates and obtains hickory chick cultivation strain in 13 days.
In addition to the implementation, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of application claims.
Claims (3)
1. a preparation technology for hickory chick bacterial classification, is characterized in that, this preparation technology's thalline comprises the following steps:
(1) female making of planting and cultivation;
Mother culture media is by the potato of 50-60 part according to the mass fraction, the agar of 8-14 part, the glucose composition of Cobastab agent 5-7 part of 0.5-0.8 part, the water that 1500ml all put into by potato, agar, Cobastab and glucose is heated, until the agar be added to the water all boiling namely to obtain mother culture media stand-by;
The mother culture media 150ml produced is got with the test tube of 250ml, test tube openend is clogged with cotton, described test tube is placed on sterilizing 1-2h in the high-pressure sterilizing pot at 115-130 DEG C, then room temperature is naturally cooled to, the aseptic inoculation box putting into sealing was tried by described, access Morciiella Esculeuta Mycelia in each test tube, be placed in 20-23 DEG C of incubator and cultivate, until grow into female kind;
(2) making of original seed and cultivation;
The composts or fertilisers of cultivating of original seed comprises following component, corn according to the mass fraction: 60-75 part, wheat: 50-60 part, paddy: 15-20 part;
The fabrication processing of the composts or fertilisers of cultivating of original seed is: weigh-soak-boil and expect-drain-packing-sealing-sterilizing-steriling test-inoculation-cultivation, wherein:
Weigh, take corn, wheat, the paddy selected respectively;
Soak, corn, wheat, paddy are soaked respectively, corn soaking 30-34h, and change a water every 5h, wheat soaks 3-4h, and paddy soaks 1-3h;
Boil material, soaked corn, wheat, paddy are placed in pot respectively and heat poach, until the seed coat of corn, wheat, paddy just breaks, then pull out, drain unnecessary moisture;
Packing, the corn after draining, wheat, paddy are loaded in the wide-mouth bottle of 100ml, it is 35-40g that every bottle of charge weight amounts to dry weight;
Sealing, point polypropylene duplicature of the wide-mouth bottle 10cm × 10cm installed is sealed a bottle hole, and cutting off a diameter at polypropylene duplicature facing to wide-mouth bottle position of bottleneck is the hole of 1.5cm, and seals with two-layer brown paper, with the fixing sealing of cotton rope;
Sterilizing, by the wide-mouth bottle sterilizing 20-25h at 120-130 DEG C after sealing, then adopts chilled air cools;
Steriling test, is placed in insulating box by the composts or fertilisers of cultivating after sterilizing and cultivates 1-3 days, if asepsis growth, sterilizing thoroughly can use; If there is bacteria growing, sterilizing is not thorough, from Amoxcillin until steriling test success;
Inoculation, aseptically, planting the mother prepared by every bottle of inoculum concentration is that 2-4g is inoculated in the test tube of composts or fertilisers of cultivating, constant temperature culture at the bacterial classification connected is placed in 23-25 DEG C;
(3) making of hickory chick bacterial classification and cultivation;
Select corncob according to the mass fraction: 75-80 part, wheat particle: 10-15 part, wood chip: 10-15 part, superphosphate: 1-3 part, urea: 1-2 part, as the composts or fertilisers of cultivating of hickory chick bacterial classification, stirs the corncob in composts or fertilisers of cultivating, wheat particle, wood chip, superphosphate, urea, maintenance water content is 75-80%, then loads in Polypropylene Bag and seals;
The Polypropylene Bag of sealing is put into autoclave sterilizing, preserves 1-3h for 100-108 DEG C under normal pressure, take out and be cooled to room temperature;
Polypropylene Bag and Primary spawn material that hickory chick strain culture-material is housed are placed in inoculating hood in the lump, and seal 0.5-1h, original seed is linked in the Polypropylene Bag that hickory chick strain culture-material is housed, and send into culturing room, the temperature controlling culturing room is 20-23 DEG C, cultivates and obtains hickory chick cultivation strain in 13-15 days.
2. the preparation technology of hickory chick bacterial classification according to claim 1, is characterized in that: the corn in described Primary spawn material, wheat, paddy all select full grains, free from insect pests, the corn without going mouldy, wheat, paddy dedusting is for subsequent use.
3. the preparation technology of hickory chick bacterial classification according to claim 1, it is characterized in that: the preparation method of the Morciiella Esculeuta Mycelia that step (1) accesses in test tube when the making that mother plants is specially: your fruit body of fresh hickory chick of gathering, and fruit body is cut into the bacterium block of 1-2mm, allow and bacterium block is positioned on PDA medium, described PDA medium is placed in the incubator of 20-23 DEG C, after 5-8 days, namely obtains Morciiella Esculeuta Mycelia.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106083338A (en) * | 2016-06-15 | 2016-11-09 | 铜仁市侗菇菌业发展有限公司 | A kind of Morchella esculenta (L.) Pers Stain activation culture medium |
| CN106544279A (en) * | 2016-11-25 | 2017-03-29 | 秦小波 | A kind of Morchella esculenta (L.) Perss cultivation strain and its induction and cultural method |
| CN108029454A (en) * | 2017-12-29 | 2018-05-15 | 崔洋 | Indoor cultivation method of morel |
| CN108076972A (en) * | 2017-12-21 | 2018-05-29 | 昆明理工大学 | A kind of method for accelerating Morciiella Esculeuta Mycelia growth |
| CN111149623A (en) * | 2020-03-06 | 2020-05-15 | 绵阳佐伊精耕科技有限公司 | Method for producing morchella cultivars in large scale |
| CN113647293A (en) * | 2021-07-20 | 2021-11-16 | 云阳县康诺百草农业科技有限公司 | Cultivation method for preventing toadstool strains from forming mycelium pellets |
| CN114532153A (en) * | 2021-12-28 | 2022-05-27 | 中国林业科学研究院亚热带林业研究所 | Open type low-cost Morchella esculenta seed production method based on aged wheat grains |
| CN115362889A (en) * | 2022-07-08 | 2022-11-22 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Method for interplanting strawberry and morchella |
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| CN106544279B (en) * | 2016-11-25 | 2019-11-08 | 秦小波 | A kind of hickory chick cultivation strain and its induction and cultural method |
| CN108076972A (en) * | 2017-12-21 | 2018-05-29 | 昆明理工大学 | A kind of method for accelerating Morciiella Esculeuta Mycelia growth |
| CN108029454A (en) * | 2017-12-29 | 2018-05-15 | 崔洋 | Indoor cultivation method of morel |
| CN111149623A (en) * | 2020-03-06 | 2020-05-15 | 绵阳佐伊精耕科技有限公司 | Method for producing morchella cultivars in large scale |
| CN113647293A (en) * | 2021-07-20 | 2021-11-16 | 云阳县康诺百草农业科技有限公司 | Cultivation method for preventing toadstool strains from forming mycelium pellets |
| CN114532153A (en) * | 2021-12-28 | 2022-05-27 | 中国林业科学研究院亚热带林业研究所 | Open type low-cost Morchella esculenta seed production method based on aged wheat grains |
| CN114532153B (en) * | 2021-12-28 | 2023-01-17 | 中国林业科学研究院亚热带林业研究所 | Open type low-cost Morchella esculenta seed production method based on aged wheat grains |
| CN115362889A (en) * | 2022-07-08 | 2022-11-22 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Method for interplanting strawberry and morchella |
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