CN107896824A - A kind of cultural method of Termitomyces albuminosus with black skin - Google Patents
A kind of cultural method of Termitomyces albuminosus with black skin Download PDFInfo
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- CN107896824A CN107896824A CN201711225739.2A CN201711225739A CN107896824A CN 107896824 A CN107896824 A CN 107896824A CN 201711225739 A CN201711225739 A CN 201711225739A CN 107896824 A CN107896824 A CN 107896824A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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Abstract
A kind of cultural method of Termitomyces albuminosus with black skin, including following steps in sequence：The preparation of parent species, four part of the preparation of original seed, the preparation of cultigen and artificial cultivation, parent species are made in mother culture media, parent species are accessed original seed is made in pedigree seed culture medium, ripe original seed is seeded in Cultivar culture medium and obtains cultigen, Termitomyces albuminosus with black skin most is made through artificial cultivation afterwards, realizes the extensive artificial cultivation of Termitomyces albuminosus with black skin, disclosure satisfy that great market demand of the people to Termitomyces albuminosus with black skin, and production process is simple, easily realizes, production cost is low.
Description
Technical field
The invention belongs to edible fungi culture technique field, and in particular to a kind of cultural method of Termitomyces albuminosus with black skin.
Background technology
Termitomyces albuminosus with black skin category Agaricales, the wild edible fungus that Tricholomataceae is temperate zone and subtropical zone summer and autumn grows, chicken
One kind of fir bacterium.Since its growth development and the activity of odontoterme have substantial connection, and, manually culture strong to growing location dependence
It is required that it is highly difficult big, therefore the artificial culture success rate of current Termitomyces albuminosus with black skin is low and of high cost.
And collybia albuminosa fine and tender taste, smell giving off a strong fragrance, delicious flavour, it is one of famous wild edible mushroom, taste is especially with black
Skin collybia albuminosa is best.The traditional Chinese medical science thinks collybia albuminosa flat property and sweet taste, there is the effect of strengthening the spleen and replenishing qi, stimulating the appetite and refreshing, analgesic oedema, is treatment
The very good food of hemorrhoid.Modern medicine finds that chicken fir to hemorrhoid in addition to having special efficacy, moreover it is possible to prevents intestinal cancer, reduces blood pressure, strengthens
Body immunity, picks merely wild Termitomyces albuminosus with black skin and has been difficult to meet the huge market demand.A kind of it is, therefore, desirable to provide energy
The method of enough extensive manually culture Termitomyces albuminosus with black skin, to meet the eating requirements of people.
The content of the invention
Based on the above prior art, it is an object of the invention to provide a kind of incubation is simple, success rate is high, cost is low
And the cultural method of the Termitomyces albuminosus with black skin suitable for large-scale culture, it is artificial on a large scale to Termitomyces albuminosus with black skin in the prior art to solve
The vacancy of culture technique, meets the eating requirements of people.
In order to realize the above object the technical solution adopted by the present invention is：
A kind of cultural method of Termitomyces albuminosus with black skin, the cultural method are included following steps in sequence：The preparation of parent species, the system of original seed
Standby, cultigen preparation and artificial cultivation, are made parent species in mother culture media, and parent species are accessed in pedigree seed culture medium and are made
Original seed, ripe original seed is seeded in Cultivar culture medium cultigen is made, Termitomyces albuminosus with black skin most is made through artificial cultivation afterwards；
The preparation of wherein parent species comprises the following steps：
Take tissue：The fructification of Termitomyces albuminosus with black skin is separated under aseptic condition, and takes cap and the bacterium performance between stable, thin
The active setup action fructification mycelium of born of the same parents；
Culture：By fructification mycelium be transferred to equipped with sterilizing liquid medium test tube at a temperature of 23-25 DEG C into
Row culture, treats to left floating a large amount of small mycelium pellets in liquid, that is, obtains parent species；
The component of wherein described liquid medium is：Murphy juice 100-300ml, agar 20g, glucose 20g, vitamin B1
5mg, magnesium sulfate 5mg.
The preparation process of the original seed is：The access of cultured parent species is equipped with the 750ml wide-mouth bottles of pedigree seed culture medium,
Being cultivated at a temperature of 25 DEG C 20-25 days can be ripe；
The component and mass fraction of wherein described pedigree seed culture medium be：50 parts of sawdust, 30-40 parts of cotton seed hulls, 5-6 parts of corn flour,
2-3 parts of wheat bran, 3-4 parts of lime, water content 40-60%.
Further, the preparation process of the cultigen is the numerous process of expansion of original seed, wherein Cultivar culture medium into
Divide and mass fraction is：50 parts of sawdust, 0.1-0.5 parts of termitarium liquid, 30-40 parts of cotton seed hulls, 5-6 parts of corn flour, wheat bran 2-3
Part, 3-4 parts of lime, water content 40-60%.
Further, the cultigen preparation process is：Above-mentioned Cultivar culture medium is loaded into culture polybag sterilizing, is gone out
Bacterium process choosing pressure is 1.5kg/cm2When lower autoclaving 2.5-3 is small and then cooling, the original that original seed preparation process is obtained
Bacteria stick is made after being seeded to culture polybag in kind；Bacteria stick is placed in culturing room and is cultivated, cultivation temperature is 15-23 DEG C, and the time is
10-15 days, cultivate to annesl and show that physiological maturity can be transferred to the soil covering culture stage.
Further, the artificial cultivation process is：Treat the bacteria stick culture of cultigen preparation process to casting skin chicken fir
After bacterium mycelia physiological maturity, bacteria stick removes polybag earthing 6-10cm cultivations, and growing temperatures are 15-20 DEG C, humidity 65-
70%；In order to preferably stimulate the formation of fructification and development, day and night temperature control is at 8-13 DEG C during soil covering culture, the pH of soil
It is worth in the range of 6.0-7.5 neutrality meta-alkalescences, to cultivate 30-35 days, you can fruiting harvests.
Compared with prior art, the invention has the advantages that：
1. realizing the extensive artificial cultivation of Termitomyces albuminosus with black skin, the shadow that field picking brings wild environment can have both been reduced
Ring, and can meet great market demand of the people to Termitomyces albuminosus with black skin；
2. take tissue, cap and bacterium performance stabilization, the institutional framework of cell mobilization between are selected, effectively raises heredity
Stability and reduce miscellaneous bacteria；
3. production process is simple, easily realizes, production cost is low, and by scientific management, enhances manually culture Termitomyces albuminosus with black skin
Quality.
Embodiment
The cultural method of the Termitomyces albuminosus with black skin of the present invention is explained with reference to specific examples below, but not as
Limitation of the invention.
Embodiment 1
1. the preparation of parent species：
Take tissue：The fructification of Termitomyces albuminosus with black skin is separated under aseptic condition, and takes cap and the bacterium performance between stable, thin
The active setup action fructification mycelium of born of the same parents；
Culture：Fructification mycelium is transferred in the test tube of the liquid medium equipped with sterilizing and is trained at a temperature of 23 DEG C
Support, until left floating a large amount of small mycelium pellets in liquid, obtain parent species；
The component of wherein described liquid medium is：Murphy juice 150ml, agar 20g, glucose 20g, vitamin B1 5mg, sulphur
Sour magnesium 5mg.
2. the preparation of original seed：
By in 750ml wide-mouth bottles of the cultured parent species access equipped with pedigree seed culture medium, 25 days are cultivated at a temperature of 25 DEG C extremely
Maturation, obtains original seed；
The component and mass fraction of the pedigree seed culture medium be：50 parts of sawdust, 30 parts of cotton seed hulls, 6 parts of corn flour, 3 parts of wheat bran, stone
4 parts of ash, water content 50%.
3. the preparation of cultigen：
Pack：Culture medium for cultivating loads 17 × 35 × 0.05 (cm) polypropylene plastics pocket, per packed 200g；
Sterilizing：Sterilization pressure 1.5kg/cm2 Lower autoclaving 2.5 thoroughly kills miscellaneous bacteria when small；
Inoculation：Bacteria stick is made after the original seed that original seed preparation process obtains is seeded to culture polybag；
Culture：Bacteria stick is moved into culturing room lucifuge culture at 18 DEG C and cultivates annesl after 15 days, that is, shows physiological maturity.
The component and mass fraction of the Cultivar culture medium be：100 parts of sawdust, 0.5 part of termitarium liquid, cotton seed hulls 60
Part, 12 parts of corn flour, 6 parts of wheat bran, 8 parts of lime, water content 50%.
4. artificial cultivation：
Furrow to be built up in mushroom shed, the polybag of bacteria stick is removed, 3-4cm occupies rear earthing 8cm above between bacteria stick and bacteria stick,
Soil is advisable (fine earth pH values 6.5) with holding the agglomerating natural soils that can be dissipated that fall on the ground, 20 DEG C of temperature of shed, day and night temperature control
10 DEG C or so, humidity is maintained at 67% or so.The scattering light mycelia leans on the nutrient and humiture of soil, about earthing 32 days
Bacteria stick forms former base and grows very long rhizoid again afterwards, and former base top expands to form mushroom flower bud, emits unearthed face and passes through 7 days or so again
Growth, it is long to 4 batches of mushrooms, biological efficiency 70% are received in picking altogether in time during 8-10cm.
Embodiment 2
1. the preparation of parent species：
Take tissue：The fructification of Termitomyces albuminosus with black skin is separated under aseptic condition, and takes cap and the bacterium performance between stable, thin
The active setup action fructification mycelium of born of the same parents；
Culture：Fructification mycelium is transferred in the test tube of the liquid medium equipped with sterilizing and is trained at a temperature of 25 DEG C
Support, until left floating a large amount of small mycelium pellets in liquid, obtain parent species；
The component of wherein described liquid medium is：Murphy juice 200ml, agar 20g, glucose 20g, vitamin B1 5mg, sulphur
Sour magnesium 5mg.
2. the preparation of original seed：
By in 750ml wide-mouth bottles of the cultured parent species access equipped with pedigree seed culture medium, 23 days are cultivated at a temperature of 25 DEG C extremely
Maturation, obtains original seed；
The component and mass fraction of the pedigree seed culture medium be：50 parts of sawdust, 40 parts of cotton seed hulls, 5 parts of corn flour, 2 parts of wheat bran, stone
3 parts of ash, water content 60%.
3. the preparation of cultigen：
Pack：Culture medium for cultivating loads 17 × 35 × 0.05 (cm) polypropylene plastics pocket, per packed 200g；
Sterilizing：Sterilization pressure 1.5kg/cm2 Lower autoclaving 2.5 thoroughly kills miscellaneous bacteria when small；
Inoculation：Bacteria stick is made after the original seed that original seed preparation process obtains is seeded to culture polybag；
Culture：Bacteria stick is moved into culturing room lucifuge culture at 20 DEG C and cultivates annesl after 13 days, that is, shows physiological maturity.
The component and mass fraction of the Cultivar culture medium be：100 parts of sawdust, 0.4 part of termitarium liquid, cotton seed hulls 80
Part, 10 parts of corn flour, 4 parts of wheat bran, 3 parts of lime, water content 60%.
4. artificial cultivation：
Furrow to be built up in mushroom shed, the polybag of bacteria stick is removed, 3-4cm occupies rear earthing 6cm above between bacteria stick and bacteria stick,
Soil is advisable (fine earth pH values 7.0) with holding the agglomerating natural soils that can be dissipated that fall on the ground, 18 DEG C of temperature of shed, day and night temperature control
10 DEG C or so, humidity is maintained at 68% or so.The scattering light mycelia leans on the nutrient and humiture of soil, about earthing 30 days
Bacteria stick forms former base and grows very long rhizoid again afterwards, and former base top expands to form mushroom flower bud, emits unearthed face and passes through 7 days or so again
Growth, to during 8-10cm in time picking altogether receive 4 batches of mushrooms, biological efficiency 80%.
Embodiment 3
1. the preparation of parent species：
Take tissue：The fructification of Termitomyces albuminosus with black skin is separated under aseptic condition, and takes cap and the bacterium performance between stable, thin
The active setup action fructification mycelium of born of the same parents；
Culture：Fructification mycelium is transferred in the test tube of the liquid medium equipped with sterilizing and is trained at a temperature of 24 DEG C
Support, until left floating a large amount of small mycelium pellets in liquid, obtain parent species；
The component of wherein described liquid medium is：Murphy juice 250ml, agar 20g, glucose 20g, vitamin B1 5mg, sulphur
Sour magnesium 5mg.
2. the preparation of original seed：
By in 750ml wide-mouth bottles of the cultured parent species access equipped with pedigree seed culture medium, 24 days are cultivated at a temperature of 25 DEG C extremely
Maturation, obtains original seed；
The component and mass fraction of the pedigree seed culture medium be：50 parts of sawdust, 35 parts of cotton seed hulls, 6 parts of corn flour, 3 parts of wheat bran, stone
4 parts of ash, water content 55%.
3. the preparation of cultigen：
Pack：Culture medium for cultivating loads 17 × 35 × 0.05 (cm) polypropylene plastics pocket, per packed 200g；
Sterilizing：Sterilization pressure 1.5kg/cm2 Lower autoclaving 3 thoroughly kills miscellaneous bacteria when small；
Inoculation：Bacteria stick is made after the original seed that original seed preparation process obtains is seeded to culture polybag；
Culture：Bacteria stick is moved into culturing room lucifuge culture at 18 DEG C and cultivates annesl after 15 days, that is, shows physiological maturity.
The component and mass fraction of the Cultivar culture medium be：100 parts of sawdust, 0.4 part of termitarium liquid, cotton seed hulls 70
Part, 12 parts of corn flour, 6 parts of wheat bran, 8 parts of lime, water content 55%.
4. artificial cultivation：
Furrow to be built up in mushroom shed, the polybag of bacteria stick is removed, 3-4cm occupies rear earthing 8cm above between bacteria stick and bacteria stick,
Soil is advisable (fine earth pH values 7.0) with holding the agglomerating natural soils that can be dissipated that fall on the ground, 18 DEG C of temperature of shed, day and night temperature control
10 DEG C or so, humidity is maintained at 70% or so.The scattering light mycelia leans on the nutrient and humiture of soil, about earthing 30 days
Bacteria stick forms former base and grows very long rhizoid again afterwards, and former base top expands to form mushroom flower bud, emits unearthed face and passes through 7 days or so again
Growth, to during 8-10cm in time picking altogether receive 4 batches of mushrooms, biological efficiency 75%.
By above-described embodiment and mushroom amount is adopted, passes through the casting skin of the cultural method culture of the Termitomyces albuminosus with black skin of the present invention
Collybia albuminosa biological efficiency is above 70%, suitable for mass production.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To modify to the technical solution described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent replacement, improvement and so on, should be included in the present invention's
Within protection domain.
Claims (4)
1. a kind of cultural method of Termitomyces albuminosus with black skin, it is characterised in that the cultural method is included following steps in sequence：Parent species
Preparation, the preparation of original seed, the preparation of cultigen and artificial cultivation, are made parent species in mother culture media, parent species are accessed former
Original seed is made in kind culture medium, and ripe original seed is seeded to Cultivar culture medium obtains cultigen, is most made afterwards through artificial cultivation
Termitomyces albuminosus with black skin；
The preparation of wherein parent species comprises the following steps：
Take tissue：The fructification of Termitomyces albuminosus with black skin is separated under aseptic condition, and takes cap and the bacterium performance between stable, thin
The active setup action fructification mycelium of born of the same parents；
Culture：By fructification mycelium be transferred to equipped with sterilizing liquid medium test tube at a temperature of 23-25 DEG C into
Row culture, treats to left floating a large amount of small mycelium pellets in liquid, that is, obtains parent species；
The component of wherein described liquid medium is：Murphy juice 100-300ml, agar 20g, glucose 20g, vitamin B1 5mg、
Magnesium sulfate 5mg;
The preparation process of the original seed is：The access of cultured parent species is equipped with the 750ml wide-mouth bottles of pedigree seed culture medium, 25
Being cultivated at a temperature of DEG C 20-25 days can be ripe；
The component and mass fraction of wherein described pedigree seed culture medium be：50 parts of sawdust, 30-40 parts of cotton seed hulls, 5-6 parts of corn flour,
2-3 parts of wheat bran, 3-4 parts of lime, water content 40-60%.
2. the cultural method of Termitomyces albuminosus with black skin according to claim 1, it is characterised in that the Cultivar culture medium into
Divide and mass fraction is：50 parts of sawdust, 0.1-0.5 parts of termitarium liquid, 30-40 parts of cotton seed hulls, 5-6 parts of corn flour, wheat bran 2-3
Part, 3-4 parts of lime, water content 40-60%.
3. the cultural method of Termitomyces albuminosus with black skin according to claim 1 or 2, it is characterised in that prepared by the cultigen
Cheng Wei：Cultivar culture medium is loaded into culture polybag sterilizing, sterilization process selection pressure is 1.5kg/cm2Lower autoclaving
When 2.5-3 is small, then cool down, bacteria stick is made after the original seed that original seed preparation process obtains is seeded to culture polybag；By bacteria stick
It is placed in culturing room and cultivates, cultivation temperature is 15-23 DEG C, and the time is 10-15 days, cultivates to annesl and shows that physiological maturity can
It is transferred to the artificial cultivation stage.
4. the cultural method of Termitomyces albuminosus with black skin according to claim 3, it is characterised in that the artificial cultivation process is：
After the bacteria stick culture to the Termitomyces albuminosus with black skin mycelia physiological maturity of cultigen preparation process, bacteria stick is removed into polybag earthing
6-10cm is cultivated, and growing temperatures are 15-20 DEG C, humidity 65-70%；In order to preferably stimulate the formation of fructification and hair
Educate, day and night temperature control is at 8-13 DEG C during soil covering culture, and the pH values of soil is in the range of 6.0-7.5 neutrality meta-alkalescences, cultivate
30-35 days, you can fruiting harvests.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108812057A (en) * | 2018-06-25 | 2018-11-16 | 安徽农业大学 | A kind of fluid nutrient medium and cultural method for being cultivated in collybia albuminosa room |
| CN109983985A (en) * | 2019-05-12 | 2019-07-09 | 湖南省食用菌研究所 | It is a kind of to cultivate formula and preparation method thereof and cultural method by the casting skin chicken fir of main formula of reed slag |
| CN111213547A (en) * | 2018-11-26 | 2020-06-02 | 深圳市鼎宸生物科技有限公司 | Cultivation method of new black-skin termitomyces albuminosus |
| CN111837809A (en) * | 2020-07-27 | 2020-10-30 | 芜湖野树林生物科技有限公司 | Black termitomyces albuminosus culture medium and preparation method and application thereof |
| CN112913576A (en) * | 2021-03-03 | 2021-06-08 | 梅州市农林科学院微生物研究所 | Cultivation method of black-skin termitomyces albuminosus |
| CN112913578A (en) * | 2021-04-02 | 2021-06-08 | 河源市农产品流通中心有限公司 | Planting and cultivating method of black-skin termitomyces albuminosus |
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| CN101218876A (en) * | 2007-12-26 | 2008-07-16 | 孙思国 | Termitomyces albuminosus with black skin producing process |
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
| EP2939526A1 (en) * | 2014-05-01 | 2015-11-04 | Van den Boomen, Henricus Nicolaas Cornelis | Method for processing casing soil |
| CN105493885A (en) * | 2015-11-24 | 2016-04-20 | 金乡县联盛菌业科技有限公司 | Culture method of black termitomyces albuminosus |
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| CN101218876A (en) * | 2007-12-26 | 2008-07-16 | 孙思国 | Termitomyces albuminosus with black skin producing process |
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
| EP2939526A1 (en) * | 2014-05-01 | 2015-11-04 | Van den Boomen, Henricus Nicolaas Cornelis | Method for processing casing soil |
| CN105493885A (en) * | 2015-11-24 | 2016-04-20 | 金乡县联盛菌业科技有限公司 | Culture method of black termitomyces albuminosus |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108812057A (en) * | 2018-06-25 | 2018-11-16 | 安徽农业大学 | A kind of fluid nutrient medium and cultural method for being cultivated in collybia albuminosa room |
| CN111213547A (en) * | 2018-11-26 | 2020-06-02 | 深圳市鼎宸生物科技有限公司 | Cultivation method of new black-skin termitomyces albuminosus |
| CN109983985A (en) * | 2019-05-12 | 2019-07-09 | 湖南省食用菌研究所 | It is a kind of to cultivate formula and preparation method thereof and cultural method by the casting skin chicken fir of main formula of reed slag |
| CN111837809A (en) * | 2020-07-27 | 2020-10-30 | 芜湖野树林生物科技有限公司 | Black termitomyces albuminosus culture medium and preparation method and application thereof |
| CN112913576A (en) * | 2021-03-03 | 2021-06-08 | 梅州市农林科学院微生物研究所 | Cultivation method of black-skin termitomyces albuminosus |
| CN112913578A (en) * | 2021-04-02 | 2021-06-08 | 河源市农产品流通中心有限公司 | Planting and cultivating method of black-skin termitomyces albuminosus |
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