CN106544279A - A kind of Morchella esculenta (L.) Perss cultivation strain and its induction and cultural method - Google Patents
A kind of Morchella esculenta (L.) Perss cultivation strain and its induction and cultural method Download PDFInfo
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- CN106544279A CN106544279A CN201611054616.2A CN201611054616A CN106544279A CN 106544279 A CN106544279 A CN 106544279A CN 201611054616 A CN201611054616 A CN 201611054616A CN 106544279 A CN106544279 A CN 106544279A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The invention discloses a kind of Morchella esculenta (L.) Perss cultivation strain and its induction and cultural method, carry out Morchella esculenta (L.) Perss induction using Morchella esculenta (L.) Perss cultivation strain parent species inducing culture, carry out Gaster caprae seu Ovis bacteria cultivation using Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating；Abductive approach includes：Test tube kind will be preserved to take out, parent species inducing culture flat board is inoculated in, is placed in 18 DEG C～22 DEG C incubator cultures, after the full plate of mycelia, as the mycelia of inoculation, be ready for use on culture medium for cultivating；Cultural method includes：Sterilizing, inoculation, the management in two stages of mycelial growth phase and sporophore growth phase.The present invention gropes to inquire into through prolonged test, there is provided a kind of at home and abroad to have breakthrough Morchella esculenta (L.) Perss artificial cultivation technique method, is a kind of optimization induction Morchella esculenta (L.) Perss strain, and makes its stable yields and quantity-produced integrated technique, realizes and commercially produce.
Description
Technical field
The invention belongs to Morchella esculenta (L.) Perss batch production, commercialization culture field, more particularly to a kind of Morchella esculenta (L.) Perss cultivation strain and its
Induction and cultural method.
Background technology
Morchella esculenta (L.) Perss Morchella esculenta (L.) Pers is under the jurisdiction of Morchellaceae (Morchellaceae) Morchella esculenta (L.) Perss
Category (Morchella), is internationally famous Precious Edible Fungi, and world's situation of selling well is Chinese main exit to European countries such as France
Edible fungus.And as the country such as moral, method, meaning, U.S. increases to the demand of Morchella esculenta (L.) Perss, its price constantly rises.Sheep
Bacterium is recorded in tripe earliest《Compendium of Materia Medica》, China utilize it is with a long history.Modern science find Morchella esculenta (L.) Perss in rich in proteins,
Fatty acid and carbohydrate, particularly various rare amino acids (as cis- 3- Amino-L-prolines, 2- aminoisobutyric acids, 2,
4- triamido isopropylformic acid .s etc.), mineral element (potassium, phosphorus, magnesium, calcium, ferrum, zinc, copper, manganese etc.) and multivitamin (thiamine, core yellow
Element, niconacid, pantothenic acid, Folic Acid, pyridoxol, biotin, ascorbic acid, VB12) etc. composition impart the unique local flavor of Morchella esculenta (L.) Perss
And nutritive value, become the high-grade food materials in international food and drink.Also, Morchella esculenta (L.) Perss Medicine Food Homology, modern medicine study show, Gaster caprae seu Ovis
Bacterium can the kidney invigorating, supplementing the brain, tonifying YANG, refresh oneself, blood fat reducing, the effect such as antitumor.In addition, Morchella esculenta (L.) Perss can be used for raw material of steeping in wine, its liquid
Body fermentation culture medium can be used for production of flavoring agent etc. again, therefore which has wide food, health product, medication chemistry etc. are multi-field
Application prospect.At present, the Morchella esculenta (L.) Perss of Chinese exports are adopted mostly in naturally wild, and which far can not meet the country and international market
Demand, and the excessive collection of wild resource causes its yield drastically to reduce, and greatly destroys the bio-diversity of Morchella esculenta (L.) Perss.
Morchella esculenta (L.) Perss are the Precious Edible Fungis of the end of spring and the beginning of summer birth.The history of the existing last 100 yearses of artificial culture Morchella esculenta (L.) Perss is explored, but, until
Ability in 1986 is existed first by the Ron Ower of biology department of san francisco, usa state university herbarium《Mycology magazine》It is upper to send out
Table artificial culture is successfully reported, and achieves two United States Patent (USP)s of Gaster caprae seu Ovis bacteria cultivation respectively nineteen eighty-two and 1986.
Before this, the successful example of Morchella esculenta (L.) Perss artificial culture is all had been reported that both at home and abroad, but simply have Morchella esculenta (L.) Pers sporophore to grow once in a while.
In recent years, China scientific worker has carried out many explorations to the artificial culture of Morchella esculenta (L.) Perss, has cultivated Morchella esculenta (L.) Pers sporophore, and
The physiological condition that some sporophore occur is inquired into.But artificial culture belongs to bionic cultivation at present, need to complete in outdoor, while
Poor repeatability, fruiting are unstable, can not carry out the commercialization cultivation of maturation so far.Therefore, the artificial or semi-artificial cultivation of Morchella esculenta (L.) Perss
Training technology is always the focus of international edible fungi research.
In sum, can not complete indoors in prior art Morchella esculenta (L.) Perss artificial culture, while the Morchella esculenta (L.) Perss for preparing repeat
Property it is poor, fruiting is unstable, it is impossible to carry out maturation commercialization cultivation.
The content of the invention
It is an object of the invention to provide a kind of Morchella esculenta (L.) Perss cultivation strain and its induction and cultural method, it is intended to solve existing
The Morchella esculenta (L.) Perss poor repeatability that can not complete indoors in planting technology, prepares, fruiting is unstable, can not carry out commercialization cultivation
Problem.
The present invention is achieved in that a kind of Morchella esculenta (L.) Perss cultivation strain parent species inducing culture, the Gaster caprae seu Ovis bacteria cultivation bacterium
Parent species inducing culture component is planted by mass by peptone 2g～4g, yeast extract 3g～7g, glucose 15g～25g, agar
18g～30g, magnesium sulfate 0.2g～0.5g, potassium dihydrogen phosphate 0.2g～0.5g, potassium nitrate 1.0g～1.5g, sodium butyrate 0.1g～
0.3g, Folic Acid 0.1g～0.2g, vitamin B1 0.1g～0.3g, vitamin B6 0.1g～0.3g, vitamin C 0.1g～
0.3g；Plus distilled water to total measurement (volume) is 1000mL.
The Morchella esculenta (L.) Perss cultivation strain parent species inducing culture component is by mass by the water logging of Rhizoma Solani tuber osi 200g～250g
Extract, glucose 15g～25g, agar 18g～30g, magnesium sulfate 0.2g～0.5g, potassium dihydrogen phosphate 0.2g～0.5g, nitric acid
Potassium 1.0g～1.5g, sodium butyrate 0.1g～0.3g, Folic Acid 0.1g～0.2g, vitamin B1 0.1g～0.3g, vitamin B6
0.1g～0.3g, vitamin C 0.1g～0.3g；Plus distilled water to total measurement (volume) is 1000mL.
Another object of the present invention is to provide a kind of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating to be planted by Morchella esculenta (L.) Perss cultivation strain
Training culture medium butt and water composition；The Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating butt by mass percentage by Semen Tritici aestivi 10%～
70%th, Semen Maydiss particle 10%～70%, wood flour 10%～25%, soil 5%～15%, peptone 0.5%～2.0%, sucrose
1.0%～3.0%, potassium dihydrogen phosphate 0.1%～0.3%, magnesium sulfate 0.1%～0.3%, carbamide 0.1%～0.3%, dimension life
Plain B1 0.1%～0.3% is constituted.
Further, the Semen Tritici aestivi+Semen Maydiss total amount is 60%～80%；The water yield of addition is 50%～70%；The cultivation
Medium pH 6.0～7.5.
Another object of the present invention is to provide a kind of abductive approach of Morchella esculenta (L.) Perss cultivation strain parent species inducing culture, the mother
The abductive approach for planting inducing culture includes：
Test tube kind will be preserved to take out, parent species inducing culture flat board is inoculated in, is placed in 18 DEG C～22 DEG C incubator cultures, treat
After the full plate of mycelia, as the mycelia of inoculation, access culture medium for cultivating is ready for use on.
Another object of the present invention is to provide a kind of cultural method of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating, and the cultivation is trained
The cultural method of foster base includes：
Sterilizing：Put autoclave sterilizing after culture medium for cultivating is bottled into, at 100 DEG C, keep 8h～10h to carry out normal pressure go out
Bacterium；Or in 1.4kg/cm21.5h～2.0h is kept to carry out autoclaving under pressure；It is next on culture medium for cultivating in bottle after sterilizing
Cause, between wheat grain, have penetrating space；
Inoculation：It is when after sterilizing, bottle temperature drop is to 30 DEG C and following, ready strain after induction is accessed under aseptic conditions；
After inoculation, cultivation vial is put into temperature control cultivation house carries out two ranks of mycelial growth phase and sporophore growth phase
The management of section.
Further, the management method of the mycelial growth phase includes：
Postvaccinal material bottle, is placed in the environment of cleaning, lucifuge and cultivates, and keeps air humidity 50%～70%；
Starting stage, indoor temperature are kept for 16 DEG C～20 DEG C；After charge level is covered with Morchella esculenta (L.) Pers. Mycelium, temperature is raised to into 20 DEG C
～23 DEG C, mycelia front cover after cultivating 15～20 days grew to bottom of bottle after 20～25 days, and mycelia has thorough grasp compost, completed nutrition life
The long stage.
Further, when carrying out the management of mycelial growth phase, after many aerial hyphaes occurs in mycelia, carry out former base and form the phase
Management；Specifically include：
(1) thermal stimulation, daytime, room temperature was controlled at 20 DEG C～23 DEG C；Be 8 DEG C～12 DEG C in the evening, stimulates 6h～10h；
(2) light stimulation, intensity of illumination in 50lx～200lx, daily illumination 10h～14h；
(3) carry out relative air humidity and be maintained at 60%～75%；As humidity is too big can cause aerial hyphae excessively prosperous and shadow
Aerial hyphae in the formation of rattle entity bud bag, easily induction base, it is unfavorable to sporophore growth；Humidity is too little, easily carries culture medium
Early dehydration and affect yield；
(4) divulge information, every morning, noon, evening respectively divulge information 15min～20min, or morning, evening each ventilation 30min；In addition
If desired, will also give mechanical stimuluss, when such as aerial hyphae growth is vigorous, bud label, iron wire or draw point of sterilization etc. can be used in training
Foster primary surface interval about 1～2cm draws the deep grids of 2～4mm；To established sclerotium, sclerotium or the patch bottle wall growth on top layer
Sclerotium remove, it is appropriate to supplement carbon containing nutritional solution, day can form former base about more than 10.
Further, the method for sporophore growth period management includes：
After sporophore grows, sealed membrane is pricked several holes first, room temperature is controlled at 20 DEG C～25 DEG C；Intensity of illumination is in 500lx
Within, daily light application time is no less than 10h；Morchella esculenta (L.) Perss have stronger phototaxis, therefore after fruit-body formation, should be according to circumstances
The relative direction of appropriate adjustment culture bottle and light source, or adjustment indoor light source direction, to ensure the normal growth of sporophore, so as to
Improve yield；Relative air humidity is maintained at 70%～90%, and highest is no more than 95%；Ventilation time is according to sporophore daily
Growth and be continuously increased ventilation time；This sporophore growth period management phases-time is 15～20 days.
Further, after one batch of Morchella esculenta (L.) Pers is harvested, as culture medium also has nutriment, keep the pipe of sporophore growth phase
Reason, carries out regrowth hair Morchella esculenta (L.) Perss management.
The Morchella esculenta (L.) Perss that the present invention is provided are a kind of Precious Edible Fungis for having very big DEVELOPMENT PROSPECT, and the present invention is through prolonged
Test gropes to inquire into, there is provided a kind of at home and abroad to have breakthrough, can make the factory culture of Morchella esculenta (L.) Perss full season indoors
Technical method, be a kind of optimization induction Morchella esculenta (L.) Perss strain, reach factory culture requirement, while bionical compared to existing outdoor
Cultivation only one season fruiting backward situation so as to full season quantity-produced integrated technique, realize and commercially produce.It is existing
Have technology at outdoor 1 year only one season fruiting, the present invention is continuous indoors to be produced, and fruiting is limited without number of times.
Description of the drawings
Fig. 1 is the cultural method flow chart of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating provided in an embodiment of the present invention.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein is not used to only to explain the present invention
Limit the present invention.
Below in conjunction with the accompanying drawings the application principle of the present invention is described in detail.
As shown in figure 1, the cultural method of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating provided in an embodiment of the present invention, including：
S101：Sterilizing：Put autoclave sterilizing after culture medium for cultivating is bottled into, at 100 DEG C, keep 8h～10h to carry out often
Pressure sterilizing；Or in 1.4kg/cm21.5h～2.0h is kept to carry out autoclaving under pressure；After sterilizing, in bottle, culture medium for cultivating is upper and lower
Unanimously, there is penetrating space between wheat grain；
S102：Inoculation：It is when after sterilizing, bottle temperature drop is to 30 DEG C and following, access under aseptic conditions ready after inducing
Strain；
S103：After inoculation, cultivation vial is put into temperature control cultivation house carries out mycelial growth phase and sporophore growth phase
The management in two stages.
Morchella esculenta (L.) Perss cultivation strain parent species inducing culture provided in an embodiment of the present invention by mass by：Peptone 2g～
4g, yeast extract 3g～7g, glucose 15g～25g, agar 18g～30g, magnesium sulfate 0.2g～0.5g, potassium dihydrogen phosphate
0.2g～0.5g, potassium nitrate 1.0g～1.5g, sodium butyrate 0.1g～0.3g, Folic Acid 0.1g～0.2g, vitamin B1 0.1g～
0.3g, vitamin B6 0.1g～0.3g, vitamin C 0.1g～0.3g；Plus distilled water to total measurement (volume) is 1000mL.
The Morchella esculenta (L.) Perss cultivation strain parent species inducing culture component is by mass by the water logging of Rhizoma Solani tuber osi 200g～250g
Extract, glucose 15g～25g, agar 18g～30g, magnesium sulfate 0.2g～0.5g, potassium dihydrogen phosphate 0.2g～0.5g, nitric acid
Potassium 1.0g～1.5g, sodium butyrate 0.1g～0.3g, Folic Acid 0.1g～0.2g, vitamin B1 0.1g～0.3g, vitamin B6
0.1g～0.3g, vitamin C 0.1g～0.3g；Plus distilled water to total measurement (volume) is 1000mL.
Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating butt provided in an embodiment of the present invention in mass ratio by：Semen Tritici aestivi 10%～
70%th, 10%～70% (note of Semen Maydiss (particle)：Semen Tritici aestivi+Semen Maydiss overall control 60%～80%), wood flour 10%～25%, soil
Earth 5%～15%, peptone 0.5%～2.0%, sucrose 1.0%～3.0%, potassium dihydrogen phosphate 0.1%～0.3%, magnesium sulfate
0.1%～0.3%, carbamide 0.1%～0.3%, vitamin B1 0.1%～0.3%, Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating
Butt adds water and constitutes Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating.
Control water content 50%～70%, pH6.0～7.5.
The application principle of the present invention is further described with reference to the construction of temperature control cultivation house.
Temperature control cultivation house of the present invention is built：
The area in culture room is usually 15～50m2, the quantity in culture room is determined according to cultivation scale, each room four sides
Wall, ceiling, door and window paste the thick cystosepiments of 3～5cm, lay cement again, carry out anniversary cultivation after ground paving 5cm thick cystosepiment
That what is trained will also install air-conditioning and exhaust fan.Iron 4 layers of bedstead of long 2m, wide 1m, high 2.3m is put in room, ground floor is liftoff
30cm, per interlayer every 60cm.The daylight lamp of 1 25W of per layer of installation.One 15m2Room can put 5 bedsteads, cultivated area
40m2。
The application principle of the present invention is further described with reference to embodiment.
The induction of parent species inducing culture of the present invention：
Test tube kind will be preserved to take out, parent species inducing culture flat board is inoculated in, is placed in 18～22 DEG C of incubator cultures, treat bacterium
After the full plate of silk, you can make inoculation and use.
The sterilizing of culture medium for cultivating of the present invention：
Autoclave sterilizing is put after bottling at once into should, normal-pressure sterilization should keep 8～10h at 100 DEG C；Autoclaving should be
1.4kg/cm21.5～2.0h is kept under pressure.After sterilizing, culture in glassware base is unanimous between the higher and lower levels, has space between wheat grain, and wheat grain can
There is adhesion but energy is penetrating.
The inoculation of culture medium for cultivating of the present invention：
When bottle temperature drop is to 30 DEG C after sterilizing, preprepared strain is accessed under aseptic conditions.It is noted that during inoculation
Ensure sterile working, mycete or bacterium infection otherwise occurs.Fungal infection can produce different pigments, and further spread, with
Morchella esculenta (L.) Perss fight for nutrition, suppress Morchella esculenta (L.) Pers. Mycelium growth；It is smelly that bacterium infection causes culture in glassware material to send out dilute, and Morchella esculenta (L.) Perss stop life
It is long.
The mycelial growth of culture medium for cultivating of the present invention is included with management of producing mushroom：
After inoculation, cultivation vial is directly placed into into temperature control cultivation house and is managed.The present invention cultivates Morchella esculenta (L.) Perss cultivation
Management technique is divided into two stages of mycelial growth phase and sporophore growth phase.
1) management of vegetative stage
This stage needs 20～25 days, and management key is lucifuge and Low- temperature culture.Postvaccinal material bottle, should be placed in cleaning, keep away
Cultivate in the environment of light, keep air humidity 50%～70%.Key is lucifuge and Low- temperature culture.Starting stage, is to reduce miscellaneous
Bacterium pollutes, and indoor temperature is preferably maintained between 16～20 DEG C, after charge level is covered with Morchella esculenta (L.) Pers. Mycelium, temperature is raised to 20～23
DEG C, mycelia front cover after cultivating 15～20 days grows to bottom of bottle after 20～25 days, mycelia can be had thorough grasp compost, complete nutrient growth
Stage.
2) former base forms the management of phase
This stage needs 10～15 days.After more aerial hyphae occurs in mycelia, the condition needed for former base is formed should be given:
(1) thermal stimulation.Daytime, room temperature was controlled at 20～23 DEG C, and be reduced to 8～12 DEG C in the evening, and daily low temperature stimulation 6～
10h。
(2) light stimulation.Intensity of illumination in 50lx～200lx, daily illumination 10h～more than 14h.Illumination is too strong, ventilation
Difference, former base differentiation are then close, or even the aging death of mycelia.
(3) humidity.Relative air humidity is maintained at 60%～75% or so.As humidity is too big can cause aerial hyphae excessively prosperous
And aerial hyphae in the formation of impact sporophore bud bag, easily induction base, it is unfavorable to sporophore growth；Humidity is too little, easily makes culture
Base is done sth. in advance dehydration and affects yield.
(4) divulge information.Every morning, noon, evening respectively divulge information 15～20min, or morning, evening each ventilation 30min.In addition as needed
Will, mechanical stimuluss to be also given, when such as aerial hyphae growth is vigorous, bud label, iron wire or draw point of sterilization etc. can be used in culture medium
Spaced surface about 1～2cm draws the deep grids of 2～4mm；To established sclerotium, the sclerotium or patch bottle wall on top layer are grown
Sclerotium removes, appropriate to supplement carbon containing nutritional solution, day can form former base about more than 10.
3) management of sporophore growth phase：
This stage needs 15～20 days, after sporophore grows, sealed membrane is pricked several holes first, and room temperature is controlled at 20～25 DEG C,
Highest is no more than 28 DEG C.Within 500lx, daily light application time is no less than 10h to intensity of illumination.Morchella esculenta (L.) Perss have the stronger light that becomes
Property, therefore the relative direction of culture bottle and light source after fruit-body formation, according to circumstances should be suitably adjusted, or adjustment indoor light source
Direction, to ensure the normal growth of sporophore, so as to improve yield.Relative air humidity is maintained at 70%～90%, and highest is not
Can exceed that 95%.Ventilation time is continuously increased ventilation time according to the growth of sporophore daily.
The harvesting and preservation of Morchella esculenta (L.) Perss of the present invention：
When Morchella esculenta (L.) Pers sporophore length is to 10cm or so, there is pigment in cap surface when going color deeper, show sporophore into
It is ripe, can harvesting.Dry or dry below 80 DEG C of sporophore after harvesting, be sealed in plastic bag, short-term preservation is put shady and cool dry
Dry place, long-term preservation are placed in low temperature refrigerator.
The management of the regrowth hair Morchella esculenta (L.) Perss of culture medium for cultivating of the present invention：
After one batch of Morchella esculenta (L.) Pers is harvested, as culture medium also has many nutriment, the culture of sporophore growth phase is kept also
Regrowth hair Morchella esculenta (L.) Perss can be grown.
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (10)
1. a kind of Morchella esculenta (L.) Perss cultivation strain parent species inducing culture, it is characterised in that the Morchella esculenta (L.) Perss cultivation strain parent species induction
Nutrient media components by mass by peptone 2g～4g, yeast extract 3g～7g, glucose 15g～25g, agar 18g～30g,
Magnesium sulfate 0.2g～0.5g, potassium dihydrogen phosphate 0.2g～0.5g, potassium nitrate 1.0g～1.5g, sodium butyrate 0.1g～0.3g, Folic Acid
0.1g～0.2g, vitamin B1 0.1g～0.3g, vitamin B6 0.1g～0.3g, vitamin C 0.1g～0.3g；Plus distillation
Water to total measurement (volume) is 1000mL.
2. Morchella esculenta (L.) Perss cultivation strain parent species inducing culture as claimed in claim 1, it is characterised in that the Gaster caprae seu Ovis bacteria cultivation
Strain parent species inducing culture component by mass by the water extract of Rhizoma Solani tuber osi 200g～250g, glucose 15g～25g, fine jade
Fat 18g～30g, magnesium sulfate 0.2g～0.5g, potassium dihydrogen phosphate 0.2g～0.5g, potassium nitrate 1.0g～1.5g, sodium butyrate 0.1g
～0.3g, Folic Acid 0.1g～0.2g, vitamin B1 0.1g～0.3g, vitamin B6 0.1g～0.3g, vitamin C 0.1g～
0.3g；Plus distilled water to total measurement (volume) is 1000mL.
3. a kind of abductive approach of Morchella esculenta (L.) Perss cultivation strain parent species inducing culture as claimed in claim 1, it is characterised in that should
The abductive approach of Morchella esculenta (L.) Perss cultivation strain parent species inducing culture includes：
Test tube kind will be preserved to take out, parent species inducing culture flat board is inoculated in, is placed in 18 DEG C～22 DEG C incubator cultures, treat mycelia
After full plate, as the mycelia of inoculation, access culture medium for cultivating is ready for use on.
4. a kind of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating, it is characterised in that the Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating is by sheep
Tripe bacteria cultivation strainsfor cultivation culture medium butt and water composition；The Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating butt presses quality percentage
Than by Semen Tritici aestivi 10%～70%, Semen Maydiss particle 10%～70%, wood flour 10%～25%, soil 5%～15%, peptone
0.5%～2.0%, sucrose 1.0%～3.0%, potassium dihydrogen phosphate 0.1%～0.3%, magnesium sulfate 0.1%～0.3%, carbamide
0.1%～0.3%, vitamin B1 0.1%～0.3% is constituted.
5. Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating as claimed in claim 4, it is characterised in that the Semen Tritici aestivi+Semen Maydiss total amount
For 60%～80%；The water yield of addition is 50%～70%；Culture medium for cultivating pH6.0～7.5.
6. a kind of cultural method of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating as claimed in claim 4, it is characterised in that the Gaster caprae seu Ovis
The cultural method of bacteria cultivation strainsfor cultivation culture medium includes：
Sterilizing：Put autoclave sterilizing after culture medium for cultivating is bottled into, at 100 DEG C, keep 8h～10h to carry out normal-pressure sterilization；Or
In 1.4kg/cm21.5h～2.0h is kept to carry out autoclaving under pressure；After sterilizing, in bottle, culture medium for cultivating is unanimous between the higher and lower levels, wheat grain
Between have penetrating space；
Inoculation：When bottle temperature falls below 30 DEG C after sterilizing, ready strain after induction is accessed under aseptic conditions；
After inoculation, cultivation vial is put into temperature control cultivation house carries out two stages of mycelial growth phase and sporophore growth phase
Management.
7. the cultural method of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating as claimed in claim 6, it is characterised in that the mycelium
The management method of trophophase includes：
Postvaccinal material bottle, is placed in the environment of cleaning, lucifuge and cultivates, and keeps air humidity 50%～70%；
Starting stage, indoor temperature are kept for 16 DEG C～20 DEG C；After charge level is covered with Morchella esculenta (L.) Pers. Mycelium, temperature is raised to into 20 DEG C～23
DEG C, mycelia front cover after cultivating 15～20 days grew to bottom of bottle after 20～25 days, and mycelia has thorough grasp compost, completes nutrient growth rank
Section.
8. the cultural method of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating as claimed in claim 6, it is characterised in that carry out mycelium
During the management of trophophase, after many aerial hyphaes occurs in mycelia, the management that former base forms the phase is carried out；Specifically include：
(1) thermal stimulation, daytime, room temperature was controlled at 20 DEG C～23 DEG C；Be 8 DEG C～12 DEG C in the evening, stimulates 6h～10h；
(2) light stimulation, intensity of illumination in 50lx～200lx, daily illumination 10h～14h；
(3) carry out relative air humidity and be maintained at 60%～75%；
(4) divulge information, every morning, noon, evening respectively divulge information 15min～20min, or morning, evening each ventilation 30min；To being formed
Sclerotium, the sclerotium of the sclerotium on top layer or patch bottle wall growth is removed, it is appropriate to supplement carbon containing nutritional solution, day can form original about more than 10
Base.
9. the cultural method of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating as claimed in claim 6, it is characterised in that sporophore growth
The method of period management includes：
After sporophore grows, sealed membrane is pricked several holes first, room temperature is controlled at 20 DEG C～25 DEG C；Intensity of illumination within 500lx,
Light application time is no less than 10h daily；Relative air humidity is maintained at 70%～90%；Life of the ventilation time according to sporophore daily
Grow and be continuously increased ventilation time；This sporophore growth period management phases-time is 15～20 days.
10. the cultural method of Morchella esculenta (L.) Perss cultivation strain culture medium for cultivating as claimed in claim 6, it is characterised in that in harvesting one
After stubble Morchella esculenta (L.) Perss, as culture medium also has nutriment, the management of sporophore growth phase is kept, regrowth hair Morchella esculenta (L.) Perss management is carried out.
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