KR101160069B1 - Production method of functional rice with medicinal materials in mushrooms cordyceps militaris - Google Patents

Production method of functional rice with medicinal materials in mushrooms cordyceps militaris Download PDF

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KR101160069B1
KR101160069B1 KR1020110011085A KR20110011085A KR101160069B1 KR 101160069 B1 KR101160069 B1 KR 101160069B1 KR 1020110011085 A KR1020110011085 A KR 1020110011085A KR 20110011085 A KR20110011085 A KR 20110011085A KR 101160069 B1 KR101160069 B1 KR 101160069B1
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cordycepin
rice
mixture
mixing
production method
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KR1020110011085A
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Korean (ko)
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진광식
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팜스코리아영농조합법인
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Priority to KR1020110011085A priority Critical patent/KR101160069B1/en
Priority to CN2011800151932A priority patent/CN102821619B/en
Priority to PCT/KR2011/000843 priority patent/WO2012108557A1/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/196—Products in which the original granular shape is maintained, e.g. parboiled rice

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE: Functional rice containing abundant amount of cordycepin, and a producing method thereof are provided to enable users to easily grow cordyceps sinensis in rice to provide the cordycepin. CONSTITUTION: A producing method of functional rice containing abundant amount of cordycepin comprises the following steps: mixing rice and deep sea water in a ratio of 100:60-80(w/w), and inserting the mixture into a sterilizing container containing vegetable carbon fibers; sterilizing the mixture at 121 deg C for 10-30 minutes; maintaining the temperature of the mixture at 20-25 deg C; injecting 20%(w/v) of cordyceps sinensis per 500g of rice mixture after growing the cordyceps sinensis for 10-15 days in a sterilized PDA medium; cultivating the cordyceps sinensis in the mixture at 20=25 deg C for 2-8 days; radiating light using an LED lamp for 10 days to the mixture by shaking the mixture twice a day for diluting; and drying the cultivated product at 50-70 deg C for 15-30 hours. The illuminance of the LED lamp is 3,000-5,000lux.

Description

코디세핀 성분이 다량 함유된 기능성 쌀 및 그 생산방법{Production method of functional rice with medicinal materials in mushrooms Cordyceps militaris}Production method of functional rice with medicinal materials in mushrooms Cordyceps militaris}

본 발명은 동충하초의 중요 기능성 성분인 코디세핀 성분이 다량 함유된 기능성 쌀 및 그 생산방법에 관한 것으로, 더욱 상세하게는 도정된 쌀을 배지로 하여 해양 심층수와 식물성 탄소 섬유 및 LED 램프를 이용하여 효과적으로 쌀에 동충하초 균을 직접 배양 시켜 기능성 성분인 코디세핀의 함량을 높여 생산하는 방법에 관한 것이다
The present invention relates to a functional rice containing a large amount of cordycepin, an important functional component of Cordyceps sinensis, and a method of producing the same, and more particularly, using deep-sealed rice, vegetable carbon fiber, and LED lamps as a medium. The present invention relates to a method of producing a plant by incubating Cordyceps sinensis directly in rice to increase the content of cordycepin, a functional ingredient.

동충하초는 박쥐나방과의 유충에서 나온 코디셉스 시넨시스 (Cordycepes sinensis)를 지칭하는 것이었지만, 오늘날에는 곤충뿐만 아니라 거미, 균류 등에서 나오는 버섯을 총칭해서 동충하초라고 부르며, 번데기동충하초와 눈꽃동충하초는 나비목 곤충의 번데기(누에번데기)를 기주로 할 수 있다는 점에서 공통점을 가진다고 할 수 있다.Cordyceps sinensis refers to Cordycepes sinensis from the larvae of the bat moth, but today, mushrooms from spiders, fungi, etc. as well as insects are collectively called cordyceps, and pupa and snowfly are the species of lepidoptera. It can be said that they have a common point in that they can be based on pupa.

동충하초는 자연 상태에서의 자실체의 채집이 어려워 누에 번데기 등을 이용한 종균배양 및 재배방법, 그리고, 인공 배지를 이용한 대량 배양 방법에 대한 연구가 최근 들어 활발하게 이루어지고 있다.
Cordyceps sinensis is difficult to collect the fruiting body in the natural state, the research on the spawn culture and cultivation method using silkworm pupa, and the mass cultivation method using artificial medium has been actively conducted in recent years.

약용 버섯(동충하초와 상황 버섯)류의 생산에 관한 종래기술을 살펴보면, 한국특허 공개 공보 제 98-033558에는 “번데기와 곡물을 혼합하여 동충하초 자실체를 형성시키는 배양방법”, 한국 특허 공개 정보 제 97-38332호에는“ 누에 번대기를 이용한 번데기 동충하초의 종균 배양방법 및 재배방법” 등록 번호 “10-0462497-0000” “약용버섯류(동충하초 ,상황 버섯)의 균사체를 배양한 기능성 쌀의 생산 방법” 등록번호 “10-0955563-0000호” “뽕잎 추출물에 누에 동충하초 균사체 배양액의 여드름균 조절 화장품 원료”등과 같이 약용버섯류(동충하초 ,상황 버섯)의 균사체를 배양에 관한 것들이 나와 있지만 이들은 접종원을 누에 번데기 종균으로 할 경우 접종 효율이 떨어지고 배양 기간이 길어진다는 단점과 자실체 형성 능력의 차이가 심하고, 생산 시 광(빛) 조건, 환기, 습도 등의 재배 조건의 복잡함으로 대량 생산 및 효율성의 한계가 있었다. 또한 단순히 성분을 외부에 코팅하는 정도의 기술이 대부분이었다. Looking at the prior art regarding the production of medicinal mushrooms (Central Cordyceps and Situary Mushroom), Korean Patent Laid-Open Publication No. 98-033558 discloses "Cultivation method of mixing pupa and grains to form cordyceps fruiting body", Korean Patent Publication No. 97- No. 38332, "Method and Cultivation of Seedling of Chrysalis of Cordyceps sinensis Using Silkworm Chrysalis" Registration No. "10-0462497-0000" "Method of Production of Functional Rice Cultivated Mycelium of Medicinal Mushrooms (E. "10-0955563-0000" has been written about the cultivation of mycelia of medicinal mushrooms (C. cordyceps, situation mushrooms), such as "acne bacterium control cosmetic raw material of silkworm Cordyceps sinensis mycelium in mulberry leaf extract". In case of inoculation efficiency is lowered and the incubation period is longer, the difference between fruiting body formation ability is severe, The complexities of cultivation conditions such as (light) conditions, ventilation, and humidity have limited mass production and efficiency. Also, most of the techniques were simply coating the components on the outside.

본 발명에서와 같이 도정된 쌀을 배지로 하여 쌀 속 깊은 곳까지 균을 침투 시키고 동충하초의 중요 기능성 성분인 코디세핀의 성분 함량을 높이기 위하여 해양 심층수와 식물성 탄소 섬유 및 LED 램프를 이용한 기술은 지금까지 보고된 바가 없었다.As a medium of the present invention, the technology using deep seawater, vegetable carbon fiber and LED lamp to infiltrate bacteria deep into the rice and increase the content of cordycepin, which is an important functional component of Cordyceps sinensis, is used as a medium. Nothing was reported.

이에 동충하초 균을 이용한 기능성 쌀 생산에 있어서 해양 심층수와 식물성 탄소 섬유 및 LED 램프를 효과적으로 적용하여 코디세핀 성분의 함량을 높인 기능성 쌀을 제공하여 소비자들에게 유익한 메디컬 푸드를 제공할 수 있는 기술의 개발이 절실히 요구되고 있다.
Therefore, in the production of functional rice using Cordyceps fungi, the development of technology that can provide consumers with beneficial medical food by providing functional rice with high content of cordycepin by applying deep ocean water, vegetable carbon fiber and LED lamp There is an urgent need.

동충하초는 자낭균 목, 핵균강, 맥강균목, 동충하초과에 속하며, 퀴닌산의 이성체로 밝혀진 핵산물질로서 “코디세핀”이라는 물질이 들어 있는데 면역증강, 천연 항생물질로서 작용 뿐만 아니라, 성기능 강화, 피로회복, 체력증진, 혈당치 하락, 암세포 발육 억제에 탁월한 효과가 있다. 코디세핀은 D-mannitol이란 물질로 혈액 속에 들어가 순환하면서 머리의 내압을 내리고 뇌수종을 경감시키며, 눈의 안압 강하 과 이뇨작용 등을 한다.Cordyceps sinensis is belonging to asymptomatic fungus, nucleus fungus, pulmonary fungus, Cordyceps sinensis, which is identified as an isomer of quinic acid, and contains a substance called “codeispin”. It has excellent effects on improving stamina, lowering blood sugar level and inhibiting cancer cell growth. Cordycepin is a substance called D-mannitol that enters the blood and circulates, lowering head pressure, reducing hydrocephalus, and reducing eye pressure and diuresis.

본 발명은 동충하초 균을 쌀에 용이하게 배양시켜 동충하초의 코디세핀 성분이 다량 포함된 쌀을 생산하는데 그 목적이 있다.
The present invention aims to produce rice containing a large amount of cordycepin components of Cordyceps sinensis by easily cultivating Cordyceps fungi in rice.

본 발명은 상기한 바와 같은 종래의 요구에 부흥하기 위한 것으로서 동충하초의 기능성 성분인 코디세핀이 다량 포함된 기능성 쌀의 생산방법에 관한 것이다. 종래의 기술이 쌀에 단순히 균사를 입히는 것이라면 본 방법에서는 자연상태의 버섯이 가지는 특유의 색과 효능을 최대한 쌀에 발현시켜 그 효능을 높이기 위한 것으로서 생산공정이 간단하면서도 코디세핀 성분이 포함된 양질의 균사체를 쌀에 직접적으로 다량 배양할 수 있는 기술을 제공 하고자, 해양 심층수와 식물성 탄소 섬유 및 LED 램프을 이용하여 동충하초의 자실체 및 균사체를 빠른 시일 내에 다량으로 쌀에 증식시켜 코디세핀의 함량을 높인 쌀을 제공하고자 하였다.
The present invention relates to a method for producing a functional rice containing a large amount of cordycepin, which is a functional component of Cordyceps sinensis, in order to revive the conventional demand as described above. If the conventional technique is simply applying mycelium to rice, this method is to express the unique color and efficacy of natural mushrooms in rice as much as possible to increase its efficacy. In order to provide a technology that can directly incubate a large amount of mycelium in rice, the fruiting body and mycelium of Cordyceps sinensis in a large amount of rice by using deep ocean water, vegetable carbon fiber, and LED lamp in a large amount of rice to increase the content of cordycepin To provide.

본 발명은 상기와 같은 발명에 의한 동충하초의 중요 기능성 성분인 코디세핀 성분이 포함된 균사체가 쌀에 다량 증식되어진 기능성 쌀의 생산방법에 관한 것으로, 쌀이 가지고 있는 식품학 효능에 동충하초가 가지고 있는 기능성 성분의 복합체를 이룸으로써 국민 건강에 직접 도움이 될 수 있는 메디컬 푸드의 재료로서 사용이 가능한 그 효과가 큰 발명이다.
The present invention relates to a method for producing functional rice in which a mycelium containing cordycepin component, which is an important functional component of Cordyceps sinensis according to the present invention, is multiplied in rice, and has a functional ingredient that Cordyceps has in the food science efficacy of rice. It is a great invention that can be used as a material of medical food that can directly help people's health by forming a complex of.

도 1은 본 발명에 사용되는 공정도.
도 2는 코디세핀 함량 고속액체크로마토그라피(HPLC)측정 표준 곡선 그래프.
도 3은 해양 심층수처리에 따른 코디세핀 함량 표.
도 4는 식물성 탄소 섬유 함유에 따른 코디세핀 함량 표.
도 5는 엘이디(LED)램프에 따른 코디세핀 함량 표. 1 is a process chart used in the present invention.
2 is a standard curve graph of cordycepin content high performance liquid chromatography (HPLC) measurement.
3 is cordycepin content table according to deep sea water treatment.
4 is a table of cordycepin content according to the vegetable carbon fiber content.
5 is cordycepin content table according to the LED (LED) lamp.

본 발명을 첨부된 도면을 참조하여 상세히 설명하면 다음과 같다.Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

이하, 본 발명에 따른 동충하초의 성분이 포함된 기능성 쌀 생산방법은 혼합 단계, 멸균및 냉각 단계, 용기 담기 및 배양균 접종 단계, 암실 및 명실 배양 단계, 건조,포장및 제품단계를 포함 한다(도1).
Hereinafter, the functional rice production method containing the components of Cordyceps sinensis according to the present invention includes a mixing step, sterilization and cooling step, container holding and culture inoculation step, dark and clear room culture step, drying, packaging and product step (Fig. One).

상기 각 단계를 아래와 같이 상세히 설명 하면 다음과 같다. Each step will be described in detail as follows.

(1) 혼합 단계 (1) mixing step

본 단계에서는 이물질을 걸러내고 청정미(2010년, 여주산)를 백미로 도정 후 도정된 쌀과 해양 심층수를 100 : 20~100(w/w)로 혼합 후 그 혼합물 500g을 멸균 용기(1,000밀리 리터 삼각 프라스크 유리 용기)에 담아서 멸균할 때 삼각 플라스크 용기내 식물성 탄소섬유 부직포가 가로2센티×세로2센티 조각을 함유하도록 한다.
In this step, the foreign material is filtered out, the clean rice (2010, Yeoju) is polished with white rice, and the milled rice and deep sea water are mixed with 100: 20 ~ 100 (w / w), and 500g of the mixture is sterilized (1,000 milliliters). When sterilized in a Erlenmeyer flask flask, the vegetable carbon fiber nonwoven fabric in the Erlenmeyer flask vessel should contain 2 centimeters by 2 centimeters in length.

동충하초(학명: CORDYCEPS MILITARIS )는 버섯의 일종으로 이다. 보통 버섯은 흙이나 나뭇가지에서 영양을 섭취하는 것과는 달리 이 버섯은 동물성(곤충) 영양분을 먹고 자라서 겨울에는 죽은 곤충에 기생하지만 여름이 되면 버섯으로 피어난다. 우리나라를 비롯해 중국과 일본 등 전서계적으로 널리 분포되어 있다. 곤충에 침입한 동충하초 균은 세계적으로 약 8백여 종이며 우리 나라에는 약 78종이 있다고 알려져 잇다. 대표적으로는 번데기동충하초, 큰매미동충하초 큰유충방망이동충하초, 눈꽃동충하초, 벌동충하초 등이 있다. 중요한 약리작용을 하는 것은 함유하고 있는 코디세핀 (Cordycepin)은 면역증강, 천연 항생물질로서 작용 뿐만 아니라, 성기능 강화, 피로회복, 체력증진, 혈당치 하락, 암세포 발육 억제에 탁월한 효과가 있다. 한편, 동충하초에는 퀴닉산의 이성체로 밝혀진 “코디세핀” 이라는 중요한 물질(핵산 물질)이 들어 있는데, 이것이 세포의 유전정보에 관여하면서 저하된 면역기능을 활성화하여 정상 세포가 암세포로 변하는 것을 방지하는 작용을 한다고 알려져 있다 . Cordyceps sinensis, scientific name: CORDYCEPS MILITARIS ) is with a kind of mushroom. Unlike ordinary mushrooms that nourish soil or twigs, they grow on animal (insect) nutrients and parasitic to dead insects in winter, but bloom in summer. It is widely distributed throughout the world, including Korea and China and Japan. There are about 800 species of Cordyceps invaded by insects, and there are 78 species in Korea. Representatives include pupa cordyceps, large cicada cordyceps, large caterpillar cordyceps, snow flower cordyceps and bee cordyceps. The important pharmacological action is that Cordycepin (Cordycepin) contains not only immune boosting, as a natural antibiotic, but also has an excellent effect on strengthening sexual function, fatigue, physical strength, lowering blood sugar level, inhibiting cancer cell development. On the other hand, Cordyceps sinensis contains an important substance (nucleic acid substance) known as an isomer of quinic acid (nucleic acid substance), which is involved in the genetic information of the cell and activates the reduced immune function to prevent normal cells from turning into cancer cells. It is known that.

해양 심충수(심층수)란 태양광이 도달하지 않는 수심 200m 이상 깊은 곳의 바닷물로 해수자원을 의미한다. 즉, 해양심층수는 수심 200미터 아래의 깊은 바다에 있는 물인데, 표층에 있는 바닷물과는 다른 성질을 가지고 있다. 심해에 존재하는 물은 오염이 되지 않고 대신 질소 ,인, 규소와 같은 영양 무기염류가 풍부하며, 수온은 거의 2℃정도로 일정한유지하며, 그 특징으로 저온 저장성, 청정성, 미네랄성, 풍부한 영양성 등의 특징을 가지고 있다. 최근에는 수산 식품, 음료, 화장품, 의학 등 다양한 분야에서 활용도와 중요성이 커지면서 각광을 받고 있다.
Deep seawater (deep water) is a seawater that is more than 200m deep without solar light. In other words, deep ocean water is water deep in the sea below 200 meters deep, and has a different nature from that of the sea surface. Water in the deep sea is not contaminated, but instead rich in nutrients and inorganic salts such as nitrogen, phosphorus and silicon, and the water temperature is maintained at about 2 ℃, and its features include low temperature storage, cleanliness, minerality, and rich nutrition. Has characteristics. In recent years, it has been in the spotlight as its utilization and importance are increasing in various fields such as seafood, beverage, cosmetics, and medicine.

숯을 이용한 천연 식물성 탄소섬유는 진공에 가까운 상태에서 소성하여 탄화시킨 섬유로 섬유의 형상은 필터 형상의 부직포 타입은 원단 두께가 2mm, 유공면적은 일반 목탄의 4.5배이며 또한 면실은 짠 직불타입도 있다. 특징으로 탈취효과 항균, 원적외선 방사, 음이온발생, 전자파 차단효과 등의 효과를 지니고 있다. The natural vegetable carbon fiber using charcoal is carbonized by firing in a vacuum close to the shape of the fiber. The nonwoven fabric of filter shape is 2mm thick and 4.5 times the pore area of ordinary charcoal. have. It has deodorizing effect, such as antibacterial effect, far infrared radiation, anion generation and electromagnetic wave blocking effect.

해양 심층수처리에 따라 동충하초 균사체가 배양된 쌀 속의 코디세핀 함량 변화는 도3에 나타나 있고, 식물성 탄소 섬유 함유처리에 따른 동충하초 균사체가 배양된 쌀 속의 코디세핀 함량 변화는 도4에 나타나 있다.
The change in cordycepin content in rice cultured with Cordyceps mycelium according to deep seawater treatment is shown in FIG. 3, and the change in cordycepin content in rice cultured with Cordyceps mycelium according to the treatment with vegetable carbon fiber is shown in FIG. 4.

(2) 멸균 및 냉각 단계, (2) sterilization and cooling steps,

본 단계에서는 (1)의 해양 심층수와 쌀과 혼합시켜 식물성 탄소 섬유를 넣은 유리 삼각 프라스크 용기(1,000밀리리터)를 고압 살균 솥에서 121℃, 10~30분간 살균 후 끄집어내어서 자연 냉각 방법으로 내용물 온도가 20~25℃를 유지 하도록 한다.
In this step, a glass triangle plastic flask (1,000 ml) containing vegetable carbon fiber mixed with deep sea water of rice (1) and sterilized in a autoclave pot is sterilized at 121 ° C. for 10-30 minutes, and then taken out by natural cooling method. Keep the temperature at 20 ~ 25 ℃.

(3) 배양균 접종 단계,(3) culture culture inoculation step,

본 단계에서는(2)의 단계를 거친 내용물에 무균상태의 감자배지 (PDA배지)를 이용하여 동충하초균(코디세프스 밀리타리스)을 실온의 암실에서 150알피엠(RPM)의 조건에서 10~15일 액체 배양한 것을 내용물 500g 대비 20% (W/v)를 무균 상태에서 접종하도록 한다.
In this step, Cordyceps fungus (Cordicephs mitaris) is grown in a dark room at room temperature of 10 to 15 rpm using a sterile potato medium (PDA medium) in the contents of step (2) at room temperature. Incubate 20% (W / v) aseptically with 500g of the contents in one liquid culture.

(4) 암실 및 명실 배양 단계,(4) dark room and clear room culturing step,

본 단계에서는(3) 단계를 거친 내용물을 20~25℃ 정도의 온도와 70%이상의 습도가 지속적으로 유지되는 청정한 배양실에서 암실 상태로 2~8일 배양 후 다음 단계로 20~25℃ 정도의 온도와 70%이상의 습도가 지속적으로 유지되는 명실 배양으로 배양실의 조도를 1000룩스 이상으로 밝게 해주고 10일간 배양하면 빛을 받는 윗부분이 버섯균사 고유의 붉은색 또는 노란색을 띄는데 이때 1일 2회 뒤집어 주어 아랫부분이 위로 올라오도록 배양하여 균사가 완전히 고유의 색을 띄면 배양을 중단하도록 한다. In this step, the contents of step (3) are incubated for 2 to 8 days in a dark room in a clean culture room where the temperature of 20 to 25 ° C. and humidity of 70% or more are continuously maintained, followed by the temperature of 20 to 25 ° C. in the next step. And bright cultivation of the incubation chamber to more than 1000 lux with 10% cultivation of the cultivation of more than 70% of the humidity. After 10 days of incubation, the upper part receiving the light has a unique red or yellow color of mushroom mycelia. Incubate the lower part upward to stop the culture when the mycelium is completely colored.

이때 명실 배양 시 엘이디(LED)램프의 광원 세기는 400룩스 이상의 조도 하에서 빛을 쪼여 배양성을 높였는데 이에 따른 동충하초의 기능성 물질인 코디세핀 함량에 미치는 영향을 도5에 도시하였다.
At this time, the light source intensity of the LED (LED) lamp in the bright room cultivation under light intensity of 400 lux or more to increase the culture, the effect on cordycepin content, which is a functional material of Cordyceps sinensis is shown in FIG.

엘이디(LED)램프는 1990년대 미국항공우주국에서 우주정거장이나 우주선내에 승무원에게 식량을 공급하기위한 식물재배에 필요한 광합성의 광원으로 이용하기 위하여 연구되어왔다. LED는 크게 IRED(Infrared Emitting Diode)와 VLED(Visible Light Emitting Diode)로 나누어진다. 특히,VLED(Visible Light Emitting Diode)칩을 뜻하며 빨강, 녹색, 오랜지색 등이 개발되어 있다. 이 칩은 램프로 조립(LED)되어 각종 전자제품의 표시나 신호등 및 전광판 등의 광원으로 쓰인다. LED는 반도체라는 특성으로 첨단조명의 광원으로 각광받는 것은 처리속도, 전력소모, 수명 등의 제반사항에서 큰 장점 때문 이다. 한편 최근 연구에 의하면 LED광선은 인체의 피부에 흡수되어 혈액순환을 촉진시키며, 세포에 흡수되어 치료시간을 50%이상 촉진시키며 근육의 긴장을 완화시킨다고 알려져 있다.
LED lamps have been studied in the 1990s by the US Aeronautics and Space Administration as a light source for photosynthesis for plant cultivation to feed crews in space stations and spacecraft. LED is divided into Infrared Emitting Diode (IRD) and Visible Light Emitting Diode (VLED). In particular, it means a VLED (Visible Light Emitting Diode) chip, and red, green, and orange are developed. The chip is assembled into a lamp (LED) and is used as a light source for various electronic products, signs and signal boards. LED is a semiconductor, which is attracting attention as a light source of advanced lighting because of its great advantages in terms of processing speed, power consumption, and lifespan. Recent studies have shown that LED light is absorbed by the human skin to promote blood circulation, and is absorbed by cells to promote healing time by more than 50% and relieve muscle tension.

(5) 건조, 포장 및 제품단계(5) Drying, packing and product stage

본 단계에서는 배양이 완료된 기능성 쌀을 용기에서 쏟아내어서 쌀 입자들이 서로 붙지 않게 적당하게 흔들어 주어 열풍 건조기(EPD-360R, 한국)를 이용하여 50~70℃에서 15~30시간 건조 하여 최종 제품이 되도록 한다.
In this step, the cultured functional rice is poured out of the container and shaken appropriately so that the rice particles do not stick together. The final product is dried at 50-70 ° C. for 15-30 hours using a hot air dryer (EPD-360R, Korea). Be sure to

이하, 도면을 참고하여 본 발명의 바람직한 실시 예는 다음 과 같다. Hereinafter, preferred embodiments of the present invention with reference to the drawings are as follows.

(1)해양 심층수처리 실시 예(1) Example of deep sea water treatment

-코디세핀 함량 측정방법-How to measure cordycepin content

본 발명은 바람직한 실시중 하나인 코디세핀 함량 측정 표준 곡선을 구하기 위하여 표준물질 코디세핀(시그마 사)을 사용하여 고속액체크로마토그라피(HPLC, Agilent 1100, series, 미국)를 사용하였고 실험조건은 이동상은In the present invention, high-performance liquid chromatography (HPLC, Agilent 1100, series, USA) was used to obtain a standard curve for measuring content of cordycepin, which is one of the preferred embodiments, using standard cordycepin (Sigma).

0.01Mphosphatebeffer:methanol=85:15. 유속: 0.5ml/min, 온도 : 25℃, 파장 : 260nm 컬럼(Column) ODS-C18, 39ⅹ300mm, 검출기 : SPD-10A로 하였다.
0.01 Mphosphate beffer: methanol = 85:15. Flow rate: 0.5 ml / min, temperature: 25 ° C, wavelength: 260 nm column ODS-C18, 39 300 mm, detector: SPD-10A.

-코디세핀 함량 고속액체크로마토그라피(HPLC)측정 표준 곡선- Cordycepin Content High-Speed Liquid Chromatography (HPLC) Measurement Standard Curve

도면 2를 참고하면 코디세핀 함량을 측정 하기위한 고속액체크로마토그라피(HPLC)측정 표준 곡선은 머무는 시간이 26.398(코디세핀 주입함량6.25μg/mL),Referring to Figure 2, the high-speed liquid chromatography (HPLC) measurement standard curve for measuring cordycepin content has a retention time of 26.398 (cordycepin injection content 6.25 μg / mL),

26.665(코디세핀주입함량12.5μg/mL), 26.865(코디세핀 주입함량25μg/mL), 26.665 (Codycepin injection content 12.5 μg / mL), 26.865 (Codycepine injection content 25 μg / mL),

27.123(코디세핀 주입함량(50μg/mL), 27.332(코디세핀 주입함량100μg/mL) 27.123 (Cordicepin Injection Content (50μg / mL), 27.332 (Codycepin Injection Content 100μg / mL)

으로 최종 표준 곡선의 식은 y = 73483x + 7724.2 (R2 = 1)이였다. The formula in the final standard curve is y = 73483x + 7724.2 (R 2 = 1)

-해양 심층수처리에 따른 코디세핀 함량 결과--Result of cordycepin content according to deep sea water treatment

본 발명의 해양 심층수처리에 따른 코디세핀 함량 측정으로 도3과 같이 나타났다. 본 발명에 따라 해양 심층수 처리 동충하초 균사체가 배양된 쌀의 코디세핀 함량의 측정방법은 앞서 기술한 바와 같다Cordycepine content measured according to the deep seawater treatment of the present invention was shown as FIG. According to the present invention, the method for measuring the cordycepin content of rice cultured with deep seawater-treated Cordyceps mycelia is as described above.

도면 3을 참고하면 원료 쌀과 해양 심층수 혼합 비율을 100 : 20~100(w/w)하여 최종 배양된 기능성 쌀의 코디세핀 함량은 3,21~20.72(ug/g)범위로 원료쌀과 해양 심층수 혼합 비율이 100 : 60~80(w/w) 사이가 우수하였으며 60 으로 할 때가 가장 좋은 것으로 나타났다.
Referring to Figure 3, the content of cordycepin in functional rice cultured at 100: 20 to 100 (w / w) in the ratio of raw rice and deep sea water was 100, 20 to 100 (w / w), ranging from 3,21 to 20.72 (ug / g). The depth mixing ratio was excellent between 100: 60 ~ 80 (w / w), and the best ratio was obtained when set to 60.

(2)식물성 탄소 섬유처리 실시 예(2) Example of vegetable carbon fiber treatment

본 발명은 바람직한 실시중 하나인 식물성 탄소 섬유( 부직포) 함유에 따른 코디세핀 함량 측정으로 도4와 같이 나타났다. 이때 코디세핀 함량 측정방법은 해양 심층수 처리의 실시 예와 같은 방법으로 행하였다.
The present invention is shown in Figure 4 by measuring the cordycepin content according to the vegetable carbon fiber (nonwoven fabric) containing one of the preferred practice. At this time, the method of measuring the content of cordycepin was performed in the same manner as in the embodiment of the deep sea water treatment.

-식물성 탄소 섬유 함유에 따른 코디세핀 함량 -Cordycepin Content According to Vegetable Carbon Fiber Content

도면 4를 참고하면 원료 쌀과 해양 심층수 혼합 비율이 100 : 60(w/w)으로 하여 용기 내 식물성 탄소 섬유를 포함하거나 포함 하지 않을 때를 비교하여 보면 포함 시 최종 배양 기능성 쌀의 코디세핀 함량은 포함 시 20.30(ug/g), 포함 하지 않을 때는 10.21(ug/g)로 나타났다.
Referring to FIG. 4, when the ratio of raw rice and deep sea water mixing is 100: 60 (w / w), the content of cordycepin in the final cultured functional rice is included when it is included or does not include vegetable carbon fiber in the container. 20.30 (ug / g) was included and 10.21 (ug / g) was not included.

(3)LED 램프 처리 실시 예(3) LED lamp processing embodiment

본 발명은 바람직한 실시 중 하나인 엘이디(LED)램프의 밝기에 따른 코디세핀 함량 측정으로 도5와 같이 나타났다. 이때 코디세핀 함량 측정방법은 앞선 실시 예와 같이 행하였다.
The present invention is shown in Figure 5 by measuring the content of cordycepin according to the brightness of the LED (LED) lamp of one of the preferred embodiments. At this time, the content of cordycepin was measured in the same manner as in the previous example.

-엘이디(LED)램프 처리에 따른 코디세핀 함량-Cordycepin Contents by LED Lamp Treatment

도면 5를 참고하면 원료 쌀과 해양 심층수 혼합 비율이 100 : 60(w/w)으로 하여 엘이디(LED)램프 밝기를 1,000~5,000 룩스로 처리해 최종 배양된 기능성 쌀의 코디세핀 함량은 13.33~21.66(ug/g) 범위로 명실 배양 시 엘이디(LED)램프 밝기가 3000~5000 룩스 사이가 우수하였으며 그 중 3,000 룩스가 가장 좋은 것으로 나타났다. Referring to Figure 5, the ratio of raw rice and deep sea water was 100: 60 (w / w), and the LED (LED) lamp brightness was treated at 1,000 to 5,000 lux, and the final cultured cordycepin content was 13.33 to 21.66 ( ug / g), LED lamps had excellent brightness between 3000 and 5000 lux, and 3,000 lux were the best.

Claims (4)

도정된 쌀과 해양 심층수 혼합물을 혼합하여 식물성 탄소섬유가 내부에 포함 된 멸균 용기에 담는 혼합 단계;
상기 혼합 단계 후 121℃, 10~30분 조건으로 멸균하는 멸균 단계;
상기 멸균 단계 후 자연 냉각방법으로 내용물 온도가 20~25℃로 유지 하는 냉각 단계:
상기 냉각 단계 후 무균상태의 피디에이(PDA)배지에서 10~15일 동안 배양된 동충하초 균을 내용물 500g 대비 20% (W/v) 접종하는 접종단계:
상기 접종하는 단계 후 암실 습도 70%이상, 온도 20~25℃의 조건하에서 2~8일간 배양하는 암실 배양 단계;
상기 암실 배양 단계 후 습도 70%이상, 온도 20~25℃의 조건하에서 10일간 엘이디(LED)램프를 이용하여 1000 룩스 이상의 조도 하에서 내용물의 희석이 이루어지도록 1일 2회 흔들어 주며 빛을 쪼이는 명실 배양 단계;
상기 명실 배양 단계 후 50~70℃에서 15~30시간 건조하는 건조단계를 포함하는 코디세핀 성분이 다량 함유된 기능성 쌀 생산방법
Mixing the milled rice and the deep sea water mixture into a sterilized container containing vegetable carbon fibers therein;
A sterilization step of sterilization at 121 ° C. for 10 to 30 minutes after the mixing step;
Cooling step to maintain the content temperature to 20 ~ 25 ℃ by the natural cooling method after the sterilization step:
Inoculation step of inoculating 20% (W / v) of the Cordyceps fungi cultured for 10 to 15 days in a sterile PDA medium after the cooling step compared to 500 g of the contents:
A darkroom culture step of incubating for 2 to 8 days under conditions of a darkroom humidity of 70% or more and a temperature of 20 to 25 ° C after the inoculating step;
After the dark room cultivation step using a LED (lamp) lamp for 10 days under the conditions of 70% humidity, temperature 20 ~ 25 ℃ shaking for twice a day to dilute the contents under the illuminance of 1000 lux or more Culture step;
Functional rice production method containing a large amount of cordycepin components comprising a drying step of drying 15-50 hours at 50 ~ 70 ℃ after the clear room culture step
제 1항에 있어서
상기 혼합단계의 쌀과 해양 심층수 혼합은 100 : 60~80(w/w)로 혼합한 것을 특징으로 하는 코디세핀 성분이 다량 함유된 기능성 쌀 생산방법
The method of claim 1
Functional rice production method containing a large amount of cordycepin components, characterized in that the mixing of the rice and deep sea water mixing of the mixing step is 100: 60 ~ 80 (w / w)
제 2항에 있어서
상기 명실 배양 단계의 LED 조도는 3,000~5,000 룩스 인 것을 특징으로 하는 코디세핀 성분이 다량 함유된 기능성 쌀 생산방법
The method according to claim 2, wherein
The LED illuminance of the clear room culture step is functional rice production method containing a large amount of cordycepin component, characterized in that 3,000 ~ 5,000 lux
상기 청구항 1 내지 3 중 어느 한 항의 생산방법으로 생산되는 것을 특징으로 하는 코디세핀 성분이 다량 함유된 기능성 쌀
Functional rice containing a large amount of cordycepin components, characterized in that produced by the production method of any one of claims 1 to 3
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