WO2012108557A1

WO2012108557A1 - Functional rice containing a large amount of cordycepin, and method for producing same

Info

Publication number: WO2012108557A1
Application number: PCT/KR2011/000843
Authority: WO
Inventors: 진광식
Original assignee: 팜스코리아영농조합법인
Priority date: 2011-02-08
Filing date: 2011-02-09
Publication date: 2012-08-16
Also published as: CN102821619A; KR101160069B1; CN102821619B

Abstract

The present invention relates to functional rice containing a large amount of cordycepin, which is an important functional ingredient of Cordyceps militaris, and to a method for producing same. In further detail, the present invention relates to a method for producing functional rice by: mixing husked rice and deep sea water, adding the mixture to a sterilizing vessel in which plant carbon fibers have been added, and sterilizing same; cooling and inoculating Cordyceps militaris spawn, and then culturing same in a dark room at a humidity of 70% or more and a temperature of 20-25ºC; and in the next step, using an LED lamp for daylight culturing the Cordyceps militaris spawn so as to expose same to light at a brightness of 1000 lux or more for a period of 10 days, during which period the contents are shaken about 2-3 times for 1 day, and then drying the contents for 15-30 hours at 50-70ºC.

Description

Functional rice containing a large amount of cordycepin and its production method

The present invention relates to a functional rice containing a large amount of cordycepin, an important functional component of Cordyceps sinensis, and a method of producing the same, and more particularly, using deep-sealed rice, vegetable carbon fiber, and LED lamps as a medium. The present invention relates to a method of producing a plant by incubating Cordyceps sinensis directly in rice to increase the content of cordycepin, a functional ingredient.

Cordyceps sinensis refers to Cordycepes sinensis from the larvae of the bat moth, but today, mushrooms from spiders, fungi, etc. as well as insects are collectively called cordyceps, and chrysalis and snowhoppers are the species of lepidoptera. It can be said that they have a common point in that they can be based on pupa.

Cordyceps sinensis is difficult to collect the fruiting body in the natural state, the research on the spawn culture and cultivation method using silkworm pupa, and the mass cultivation method using artificial medium has been actively conducted in recent years.

Looking at the prior art regarding the production of medicinal mushrooms (Central Cordyceps and Situary Mushroom), Korean Patent Laid-Open Publication No. 98-033558 discloses "Cultivation method of mixing pupa and grains to form cordyceps fruiting body", Korean Patent Publication No. 97- No. 38332, "Method and Cultivation of Seedling of Chrysalis of Cordyceps sinensis Using Silkworm Chrysalis" Registration No. "10-0462497-0000" 10-0955563-0000 There are information on cultivating mycelium of medicinal mushrooms (Cordyceps sinensis, Situation mushroom) such as “Culgar leaf extract extract control raw material of acne bacterium mycelium culture in mulberry leaf extract”. In case of inoculation efficiency is lowered and the incubation period is longer, the difference between fruiting body formation ability is severe, The complexities of cultivation conditions such as (light) conditions, ventilation, and humidity have limited mass production and efficiency. Also, most of the techniques were simply coating the components on the outside.

As a medium of the present invention, the technology using deep seawater, vegetable carbon fiber and LED lamp to infiltrate bacteria deep into the rice and increase the content of cordycepin, which is an important functional component of Cordyceps sinensis, is used as a medium. Nothing was reported.

Therefore, in the production of functional rice using Cordyceps fungi, the development of technology that can provide consumers with beneficial medical food by providing functional rice with high content of cordycepin by applying deep ocean water, vegetable carbon fiber and LED lamp There is an urgent need.

Cordyceps sinensis is belonging to asymptomatic fungus, nucleus fungus, pulmonary fungus, Cordyceps sinensis, which is identified as an isomer of quinic acid, and contains a substance called “codeispin”. It has excellent effects on improving stamina, lowering blood sugar level and inhibiting cancer cell growth. Cordycepin is a substance called D-mannitol that enters the blood and circulates, lowering head pressure, reducing hydrocephalus, and reducing eye pressure and diuresis.

The present invention aims to produce rice containing a large amount of cordycepin components of Cordyceps sinensis by easily cultivating Cordyceps fungi in rice.

The present invention relates to a method for producing a functional rice containing a large amount of cordycepin, which is a functional component of Cordyceps sinensis, in order to revive the conventional demand as described above. If the conventional technique is simply applying mycelium to rice, this method is to express the unique color and efficacy of natural mushrooms in rice as much as possible to increase its efficacy. In order to provide a technology that can directly incubate a large amount of mycelium in rice, the fruiting body and mycelium of Cordyceps sinensis in a large amount of rice by using deep ocean water, vegetable carbon fiber, and LED lamp in a large amount of rice to increase the content of cordycepin To provide.

The present invention relates to a method for producing functional rice in which a mycelium containing cordycepin component, which is an important functional component of Cordyceps sinensis according to the present invention, is multiplied in rice, and has a functional ingredient that Cordyceps has in the food science efficacy of rice. It is a great invention that can be used as a material of medical food that can directly help people's health by forming a complex of.

1 is a process chart used in the present invention.

2 is a standard curve graph of cordycepin content high performance liquid chromatography (HPLC) measurement.

3 is cordycepin content table according to deep sea water treatment.

4 is a table of cordycepin content according to the vegetable carbon fiber content.

5 is cordycepin content table according to the LED (LED) lamp.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

Hereinafter, the functional rice production method containing the components of Cordyceps sinensis according to the present invention includes a mixing step, sterilization and cooling step, container holding and culture inoculation step, dark and clear room culture step, drying, packaging and product step (Fig. One).

Each step will be described in detail as follows.

(1) mixing step

In this step, the foreign material is filtered out, the clean rice (2010, Yeoju) is polished with white rice, and the milled rice and deep sea water are mixed with 100: 20 ~ 100 (w / w), and 500g of the mixture is sterilized (1,000 milliliters). When sterilized in a Erlenmeyer flask flask, the vegetable carbon fiber nonwoven fabric in the Erlenmeyer flask vessel should contain 2 centimeters by 2 centimeters in length.

Cordyceps sinensis ( CORDYCEPS MILITARIS ) is a type of mushroom. Unlike ordinary mushrooms that nourish soil or twigs, they grow on animal (insect) nutrients and are parasitic to dead insects in winter, but bloom in summer. It is widely distributed throughout the world, including Korea and China and Japan. There are about 800 species of Cordyceps invaded by insects and there are about 78 species in Korea. Representatives include chrysalis cordyceps, large cicada cordyceps, large caterpillar cordyceps, snow flower cordyceps and bee cordyceps. The important pharmacological action is that Cordycepin (Cordycepin) is not only effective as an immune booster, as a natural antibiotic, but also has an excellent effect on strengthening sexual function, fatigue, stamina, lowering blood sugar level and inhibiting cancer cell development. On the other hand, Cordyceps sinensis contains an important substance (nucleic acid substance) known as an isomer of quinic acid (nucleic acid substance), which is involved in the genetic information of the cell and activates the reduced immune function to prevent normal cells from turning into cancer cells. It is known that.

Deep seawater (deep water) is a seawater that is more than 200m deep without solar light. In other words, deep ocean water is water deep in the sea below 200 meters deep, and has a different nature from that of the sea surface. Water in the deep sea is not contaminated, but instead rich in nutrients and inorganic salts such as nitrogen, phosphorus and silicon, and the water temperature is maintained at about 2 ℃, and its features include low temperature storage, cleanliness, minerality, and rich nutrition. Has characteristics. In recent years, it has been in the spotlight as its utilization and importance are increasing in various fields such as seafood, beverage, cosmetics, and medicine.

The natural vegetable carbon fiber using charcoal is carbonized by firing in a vacuum close to the shape of the fiber. The nonwoven fabric of filter shape is 2mm thick and 4.5 times the pore area of ordinary charcoal. have. It has deodorizing effect, such as antibacterial effect, far infrared radiation, anion generation and electromagnetic wave blocking effect.

The change in cordycepin content in rice cultured with Cordyceps mycelium according to deep seawater treatment is shown in FIG. 3, and the change in cordycepin content in rice cultured with Cordyceps mycelium according to the treatment with vegetable carbon fiber is shown in FIG. 4.

(2) sterilization and cooling steps,

In this step, a glass triangle plastic flask (1,000 ml) containing vegetable carbon fiber mixed with deep sea water of rice (1) and sterilized in a autoclave pot is sterilized at 121 ° C. for 10-30 minutes, and then taken out by natural cooling method. Keep the temperature at 20 ~ 25 ℃.

(3) culture culture inoculation step,

In this step, Cordyceps fungus (Cordicephs mitaris) is grown in a dark room at room temperature of 10 to 15 rpm using a sterile potato medium (PDA medium) in the contents of step (2) at room temperature. Incubate 20% (W / v) aseptically with 500g of the contents in one liquid culture.

(4) dark room and clear room culturing step,

In this step, the contents of step (3) are incubated for 2 to 8 days in a dark room in a clean culture room where the temperature of 20 to 25 ° C. and humidity of 70% or more are continuously maintained, followed by the temperature of 20 to 25 ° C. in the next step. And bright cultivation of the incubation chamber to more than 1000 lux with 10% cultivation of the cultivation of more than 70% of the humidity. After 10 days of incubation, the upper part receiving the light has a unique red or yellow color of mushroom mycelia. Incubate the lower part upward to stop the culture when the mycelium is completely colored.

At this time, the light source intensity of the LED (LED) lamp in the bright room cultivation under light intensity of 400 lux or more to increase the culture, the effect on cordycepin content, which is a functional material of Cordyceps sinensis is shown in FIG.

LED lamps have been studied in the 1990s by the US Aeronautics and Space Administration as a light source for photosynthesis for plant cultivation to feed crews in space stations and spacecraft. LED is divided into Infrared Emitting Diode (IRD) and Visible Light Emitting Diode (VLED). In particular, it means a VLED (Visible Light Emitting Diode) chip, and red, green, and orange are developed. The chip is assembled into a lamp (LED) and is used as a light source for various electronic products, signs and signal boards. LED is a semiconductor, which is attracting attention as a light source of advanced lighting because of its great advantages in terms of processing speed, power consumption, and lifespan. Recent studies have shown that LED light is absorbed by the human skin to promote blood circulation, and is absorbed by cells to promote healing time by more than 50% and relieve muscle tension.

(5) Drying, packing and product stage

In this step, the cultured functional rice is poured out of the container and shaken appropriately so that the rice particles do not stick together. The final product is dried at 50-70 ° C. for 15-30 hours using a hot air dryer (EPD-360R, Korea). Be sure to

Hereinafter, preferred embodiments of the present invention with reference to the drawings are as follows.

(1) Example of deep sea water treatment

How to measure cordycepin content

In the present invention, high-performance liquid chromatography (HPLC, Agilent 1100, series, USA) was used to obtain a standard curve for measuring content of cordycepin, which is one of the preferred embodiments, using standard cordycepin (Sigma).

0.01 Mphosphate beffer: methanol = 85:15. Flow rate: 0.5 ml / min, temperature: 25 ° C., wavelength: 260 nm column ODS-C18, 39 × 300 mm, detector: SPD-10A.

Cordycepin Content High-Speed Liquid Chromatography (HPLC) Measurement Standard Curve

Referring to Figure 2, the high-speed liquid chromatography (HPLC) measurement standard curve for measuring cordycepin content has a retention time of 26.398 (cordycepin injection content 6.25 μg / mL),

26.665 (Codycepin injection content 12.5 μg / mL), 26.865 (Codycepine injection content 25 μg / mL),

27.123 (Cordicepin Injection Content (50μg / mL), 27.332 (Codycepin Injection Content 100μg / mL)

The equation for the final standard curve was y = 73483x + 7724.2 (R ² = 1).

-Result of cordycepin content according to deep sea water treatment

Cordycepine content measured according to the deep seawater treatment of the present invention was shown as FIG. According to the present invention, the method for measuring the cordycepin content of rice cultured with deep seawater-treated Cordyceps mycelia is as described above.

Referring to Figure 3, the content of cordycepin in functional rice cultured at 100: 20 to 100 (w / w) in the ratio of raw rice and deep sea water was 100, 20 to 100 (w / w), ranging from 3,21 to 20.72 (ug / g). The depth mixing ratio was excellent between 100: 60 ~ 80 (w / w), and the best ratio was obtained when set to 60.

(2) Example of vegetable carbon fiber treatment

The present invention is shown in Figure 4 by measuring the cordycepin content according to the vegetable carbon fiber (nonwoven fabric) containing one of the preferred practice. At this time, the method of measuring the content of cordycepin was performed in the same manner as in the embodiment of the deep sea water treatment.

Cordycepin Content According to Vegetable Carbon Fiber Content

Referring to FIG. 4, when the ratio of raw rice and deep sea water mixing is 100: 60 (w / w), the content of cordycepin in the final cultured functional rice is included when it is included or does not include vegetable carbon fiber in the container. 20.30 (ug / g) was included and 10.21 (ug / g) was not included.

(3) LED lamp processing embodiment

The present invention is shown in Figure 5 by measuring the content of cordycepin according to the brightness of the LED (LED) lamp of one of the preferred embodiments. At this time, the content of cordycepin was measured in the same manner as in the previous example.

Cordycepin Contents by LED Lamp Treatment

Referring to Figure 5, the ratio of raw rice and deep sea water was 100: 60 (w / w), and the LED (LED) lamp brightness was treated at 1,000 to 5,000 lux, and the final cultured cordycepin content was 13.33 to 21.66 ( ug / g), LED lamps had excellent brightness between 3000 and 5000 lux, and 3,000 lux were the best.

Claims

Mixing the milled rice and the deep sea water mixture into a sterilized container containing vegetable carbon fibers therein;
Sterilization step of sterilization at 121 ℃, 10 ~ 30 minutes condition after the mixing step;
Cooling step to maintain the content temperature to 20 ~ 25 ℃ by the natural cooling method after the sterilization step:
Inoculation step of inoculating 20% (W / v) with 500 g of Cordyceps fungi cultured in a sterile CDA medium for 10-15 days after the cooling step:
Darkroom culture step of culturing for 2 to 8 days under the conditions of 70% or more dark room humidity, temperature 20 ~ 25 ℃ after the step of inoculation;
After the dark room cultivation step using a LED (lamp) lamp for 10 days under conditions of 70% humidity, temperature 20 ~ 25 ℃ shaking light twice a day to dilute the contents under the illuminance of 1000 lux or more Culture step;
Functional rice production method containing a large amount of cordycepin components, including a drying step of drying for 15 to 30 hours at 50 ~ 70 ℃ after the clear room culture step
The method of claim 1
The rice and marine deep water mixing of the mixing step is 100: 60 ~ 80 (w / w) functional rice production method containing a large amount of cordycepin components, characterized in that the mixture
The method of claim 2
The LED illuminance of the clear room culture step is functional rice production method containing a large amount of cordycepin component, characterized in that 3,000 ~ 5,000 lux
Functional rice containing a large amount of cordycepin components, characterized in that produced by the production method of any one of claims 1 to 3