CN108450231B - A kind of bionical border expanding propagation method of wild Phellinus - Google Patents
A kind of bionical border expanding propagation method of wild Phellinus Download PDFInfo
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- CN108450231B CN108450231B CN201810217706.1A CN201810217706A CN108450231B CN 108450231 B CN108450231 B CN 108450231B CN 201810217706 A CN201810217706 A CN 201810217706A CN 108450231 B CN108450231 B CN 108450231B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
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Abstract
The present invention discloses a kind of bionical border expanding propagation method of wild Phellinus, step: wild Phellinus strain acquires and numerous selection is expanded in cultural hypha, the preparation of Phellinus Inoculant, bionical border and numerous, numerous nutritional supplementation of expansion is expanded in management, inoculation, numerous condition management is expanded in bionical border, goes out yellow management, pest management.The present invention has the advantages that the present invention is directly inoculated into old mulberry tree pier in a manner of microinjection, not only reduces Phellinus original seed preparation process, shorten Phellinus cultivation cycle, reduce contamination probability directly by wild Phellinus Phellinus spore liquid directly obtained；Simultaneously using old mulberry tree pier as Phellinus growth substrate, the primary border of wild Phellinus growth is presented completely, guarantees the good wild characteristic of Phellinus and nutritive value；In addition nutritional agents is added, facilitates Phellinus growth and growing of mulberry, guarantees the quality of Phellinus.
Description
Technical field
The present invention relates to field of agricultural cultivation, specifically a kind of wild bionical border multiplication technique of Phellinus.
Background technique
Phellinus, also known as mulberry ear are a kind of medicinal fungis that preciousness is rare ,-as parasitic growth in mulberry, poplar, willow, birch, oak etc.
On trunk or withered stake, wherein best to parasitize the Phellinus value utilitarian on mulberry tree.The Phellinus colonized in mulberry tree contains more
Sugared content highest, immune system in the polysaccharide energy human activin contained enhance human body anticancer activity, immunocompetence, have bright
Aobvious antitumous effect.Therefore, Phellinus is that the current whole world generally acknowledges that effective percentage makes number one high true in the carcinogenic medicament of biology
Bacterium has great medical value and potentiality to be exploited.
Wild Phellinus due to by physiological ecological particularity and complexity and external environment condition restricted, in nature
The fructification of middle formation is rare, and the growth cycle of Phellinus is long in addition, and leading to wild Phellinus, supply falls short of demand, at high price, to Phellinus
The stable sound development of industry is unfavorable.
Therefore, solve the problems, such as that wild resource deficiency has great importance by artificial cultivation.But due to Phellinus pair
There is very strong sensibility and specificity in environmental condition and nutritional condition, culture conditions are difficult to be fitted wild Phellinus completely
Primary border is grown, so the problems such as artificial culture is easy to cause Phellinus spawn degeneration.But with mulberry tree or other equal positions using former
Ecological pseudo-wild cultivating can effectively solve the problems, such as this.The patent of invention of grant number CN103598010 discloses a kind of Phellinus
Ecosystem pseudo-wild cultivating method, this method comprises the following steps: (1) bacterial strain separated from wild Phellinus fructification is purified
After be inoculated into mother culture media culture and obtain Phellinus parent species；(2) Phellinus parent species are inoculated into culture on pedigree seed culture medium and obtain mulberry
Xanthan kind；(3) Phellinus original seed is inoculated into culture in the wooden peg culture medium in cultivating bag and obtains wooden peg cultivar；(4) it will cover with
The wooden peg cultivar of phellinus igniarius mycelium is inoculated into progress nature culture on mulberry tree living body.This method provides a kind of new Phellinus is former
Ecological pseudo-wild cultivating method, but since its process is complex, the drilling culture of wooden peg culture kind mulberry tree is easy to living body mulberry
Tree trunk and vascular bundle etc. cause wound, influence Phellinus vegetative growth phase nutrition supply deficiency, can also cause Phellinus low output
Problem, while wound easily causes to pollute, and influences Phellinus quality, therefore need to change existing ecosystem pseudo-wild cultivating method
Into convenient for being promoted and applied after technology curing.
Summary of the invention
The purpose of the present invention is to provide a kind of easy to operate, high survival rate, go out that the sesame period is short, the relatively high Phellinus of yield
Bionical border multiplication technique.
The technical solution of the present invention is as follows: a kind of bionical border expanding propagation method of wild Phellinus, step:
(1) wild Phellinus strain acquisition and cultural hypha: the open country obtained will be acquired from the mulberry tree that the age of tree is 15 years or more
Raw Phellinus, carries out in vitro cultural hypha, cuts rectangular small having a size of 1.8-2.2cm × 0.9-1.3cm × 0.35-0.55cm
Block, after 75% ethyl alcohol surface sterilization, grinding carries out culture medium culture, culture medium prescription parts by weight are as follows: 2-3 parts of ramulus mori liquid, glucose
10-12 parts, 1-2 parts of potassium dihydrogen phosphate, 0.5-0.8 parts of magnesium sulfate, 4-6 parts of peptone, 3-5 parts of agar powder, 50mg/L streptomysin
2-3 parts, 1000 parts of water, 28 DEG C of cultivation temperature, cultivate humidity 30-45%, incubation time 3-5 days.The wherein preparation of ramulus mori liquid: it plucks
500-800 parts of new fresh mulberry leafs are taken, 100-300 parts of water are added, are ground, filtering, 300r/min room temperature centrifugation is made.
(2) prepared by Phellinus Inoculant: by the good phellinus liteus access culture medium of pure culture, it is small to carry out shaking table culture 48
When, the Phellinus bacterium solution of every milliliter of 106 spores is then made.
(3) bionical border expand numerous select and management: the bionical border of Phellinus expands numerous and selects the age of tree for 15 years or more mulberry treies
Stump, stump length are not less than 70cm, are erected on the sandy loam face of good water-retaining property, suitable shady place is advisable.Stump can directly be put
It puts together, can also individually put.
(4) inoculation is expanded numerous: before inoculation, mulberry tree pier is first carried out disinfection with pasteurized water, will be obtained after disinfection 6 hours
Concentration is the spore bacterium solution of 106/milliliter, is injected into old mulberry tree in upward 15 degree of oblique cuttings using the glass Syringe injector of 10mL
It carries out expanding above pier numerous；Injection site should select the cortex portion below mulberry fist, and each mulberry tree pier, which is staggered, is inoculated with 5-6 position, note
Using round special sterile PE protective film after penetrating, injection site is pasted, when Phellinus former base protrusion, removes PE protective film.
(5) expand numerous nutritional supplementation: after old mulberry tree pier inoculation Phellinus, within the scope of from injection point 10cm, 5ml is respectively adopted
Glass syringe, oblique cutting injects 20-25ml nutritional agents, is typically chosen in injection point two orientation injection up and down.Injection time
It is primary every injection in 15 days after inoculation Phellinus, until harvesting.Nutritional agents formulation weight are as follows: 10-12 parts of vitamin A, dimension life
Plain B 25-28 parts, 2-3 parts of vitamin D .5-2 parts of amino acid 1,5-8 parts of active fructose, 13-15 parts of glucose, zinc sulfate
20-30 parts, 35-38 parts of magnesium sulfate, 1-2 parts of triacontanol, 100-110 parts of urea, 3-4 parts of potassium dihydrogen phosphate, water 1000-1200
Part.
(6) numerous condition management is expanded in bionical border: shady and cool wet place, general control are preferably placed on after old mulberry tree pier inoculation Phellinus
At 24-28 DEG C of temperature, 80% or more humidity.Cloudy day or rainy day do not spray water, and sunny day uses spray pattern to spray 4-5 water daily, every time
8-10 minutes.
(7) go out yellow management: when former base expands to form cap, Yao Zengjia spray timings improve humidity, and humidity is kept to exist
90% or more, 20-25 DEG C of temperature.It can be harvested when Phellinus fructification maturation.
Pest management: Phellinus goes out the harm of easy fungal infection and virus and acarid after Huang, therefore generally in mulberry
Huang goes out after Huang to picking, need to periodically sprinkle 75% alcohol and be prevented, the limewash or 0.15% hundred of 4%-5% is sprayed after generation
Bacterium is prevented and treated clearly.
Points for attention:
(1) bionical border expands old mulberry tree pier in numerous bed and refers to that the age of tree is 15 years or more old mulberry treies, needs vertical display, old mulberry tree
It must facilitate air flowing, after reducing and avoid Phellinus to go out Huang, the generation of disease there are at least gap 30cm between pier.
(2) light application time and Phellinus growth are negatively correlated, therefore old mulberry tree pier is preferably placed on bower, or builds shading shed,
Must there are humidification apparatus such as spray equipment or drip irrigation system.
The present invention has the advantages that (1) is of the invention directly by wild Phellinus Phellinus spore liquid directly obtained, with microinjection
Mode is directly inoculated into old mulberry tree pier, not only reduces Phellinus original seed preparation process, shortens Phellinus cultivation cycle, reduces pollution generally
Rate；(2) simultaneously using old mulberry tree pier as Phellinus growth substrate, the primary border of wild Phellinus growth is presented completely, guarantees that Phellinus is good
Good wild characteristic and nutritive value；(3) nutritional agents is in addition added, facilitates Phellinus growth and growing of mulberry, guarantees Phellinus
Quality.The method of the present invention simplicity, quick, production cost and labor intensity are low, and educated Phellinus quality is good, the period is short, economic benefits
Good, popularization and application foreground is wide.
Detailed description of the invention
Fig. 1 is that Phellinus Inoculant is inoculated with schematic diagram；
Fig. 2 is mulberry tree pier Phellinus scene figure one；
Fig. 3 is mulberry tree pier Phellinus scene figure two.
In figure: A- mulberry tree pier, B- mulberry fist, C- Phellinus vaccination, D- Phellinus Inoculant, the micro- inoculator of E- Phellinus.
Specific embodiment
Embodiment 1:
(1) wild Phellinus strain acquisition and cultural hypha: the Wild Mulberry obtained will be acquired from the mulberry tree that the age of tree is 15 years
Huang carries out in vitro cultural hypha, cuts the rectangular fritter of 2cm × 1cm × 0.5cm, and after 75% ethyl alcohol surface sterilization, grinding is trained
Support base culture, culture medium prescription are as follows: 2 parts of ramulus mori liquid, 10 parts of glucose, 1.5 parts of potassium dihydrogen phosphate, 0.5 part of magnesium sulfate, peptone
3 parts, 3 parts of agar powder, 0.2 part of streptomysin (50mg/L), 1000 parts of water, cultivate humidity 30%, incubation time by 28 DEG C of cultivation temperature
3-5 days.The wherein preparation of ramulus mori liquid: winning 600 parts of new fresh mulberry leafs, and 180 parts of water are added, grind, filtering, 300r/min room temperature from
The heart is made.
(2) prepared by Phellinus Inoculant: by the good phellinus liteus access culture medium of pure culture, it is small to carry out shaking table culture 48
When, the Phellinus bacterium solution of every milliliter of 106 spores is then made.
(3) inoculation is expanded numerous: the age of tree being selected to be 15 years or more and expand not less than the mulberry tree stump of 70cm long as bionical border numerous
Bed, mid or late April injects Phellinus bacterium solution upward 15 degree of oblique cuttings expands above the old mulberry tree pier that 1ml is 15 years or more to the age of tree
It is numerous；Old mulberry tree pier vertical display, there are gaps between old mulberry tree pier, are conducive to ventilation；Injection site should select the skin below mulberry fist
Matter portion, each mulberry tree pier, which is staggered, is inoculated with 5-6 position.
(4) expand numerous nutritional supplementation: after old mulberry tree pier inoculation Phellinus, within the scope of from injection point 10cm, 5ml is respectively adopted
Glass syringe, oblique cutting injects 20ml nutritional agents, is typically chosen in injection point two orientation injection up and down.Injection time is to connect
It is primary every injection in 15 days after kind Phellinus, until harvesting.Nutrition agent prescription are as follows: 10 parts of vitamin A, 25 parts of vitamin B, dimension
Raw 2 parts of element D .5 parts of amino acid 1,5 parts of active fructose, 13 parts of glucose, 20 parts of zinc sulfate, 35 parts of magnesium sulfate, triacontanol 1
Part, 100 parts of urea, 3 parts of potassium dihydrogen phosphate, 1000 parts of water.
(5) numerous selection and management is expanded in bionical border: observation Hygrothermograph is set when spending for 24 ± 2 DEG C of mid or late April temperature
Expand under low light condition under tree shade or bamboo grove it is numerous, cloudy day or the rainy day do not spray water, sunny day use daily spray pattern spray 4 water, often
Secondary 8 minutes, keep humidity 80% or more.When former base expands to form cap, Yao Zengjia spray timings improve humidity, keep wet
Degree is 90% or more, temperature 20-25 degree.Late May to mid-June is the yellow phase, and when Phellinus fructification maturation can harvest.
(6) pest management: Phellinus is easy the harm of fungal infection and virus and acarid after going out Huang, therefore generally exists
Phellinus goes out after Huang to picking, need to periodically sprinkle 75% alcohol and be prevented, 4% limewash or 0.15% hundred is sprayed after generation
Bacterium is prevented and treated clearly.
Embodiment 2:
(1) strain and cultural hypha are obtained: wild Phellinus strain acquisition and cultural hypha: by the mulberry tree for being 18 years from the age of tree
The wild Phellinus that upper acquisition obtains, carries out in vitro cultural hypha, cuts the rectangular fritter of 1.9cm × 0.9cm × 0.45cm, and 75%
After ethyl alcohol surface sterilization, grinding carries out culture medium culture, culture medium prescription are as follows: 3 parts of ramulus mori liquid, 12 parts of glucose, biphosphate
1.5 parts of potassium, 0.8 part of magnesium sulfate, 5 parts of peptone, 5 parts of agar powder, 3 parts of streptomysin (50mg/L), 1000 parts of water, cultivation temperature 28
DEG C, cultivate humidity 30-45%, incubation time 3-5 days.The wherein preparation of ramulus mori liquid: winning 800 parts of new fresh mulberry leafs, is added 200 parts
Water is ground, filtering, and the centrifugation of 300r/min room temperature is made.
(2) prepared by Phellinus Inoculant: by the good phellinus liteus access culture medium of pure culture, it is small to carry out shaking table culture 48
When, the Phellinus bacterium solution of every milliliter of 106 spores is then made.
(3) inoculation is expanded numerous: the upward 15 degree of oblique cuttings of Phellinus bacterium solution being injected on the old mulberry tree pier that 1ml is 15 years or more to the age of tree
Face expand numerous；Old mulberry tree pier vertical display, there are gaps between old mulberry tree pier, are conducive to ventilation；Injection site should select mulberry fist
Following cortex portion, each mulberry tree pier, which is staggered, is inoculated with 5-6 position.
(4) expand numerous nutritional supplementation: after old mulberry tree pier inoculation Phellinus, within the scope of from injection point 10cm, 5ml is respectively adopted
Glass syringe, oblique cutting injects 25ml nutritional agents, is typically chosen in injection point two orientation injection up and down.Injection time is to connect
It is primary every injection in 15 days after kind Phellinus, until harvesting.Nutrition agent prescription are as follows: 12 parts of vitamin A, 28 parts of vitamin B, dimension
Raw 3 parts of element D, 2 parts of amino acid, 8 parts of active fructose, 15 parts of glucose, 26 parts of zinc sulfate, 38 parts of magnesium sulfate, triacontanol 1.5
Part, 105 parts of urea, 4 parts of potassium dihydrogen phosphate, 1200 parts of water.
(5) numerous condition selection and management are expanded in bionical border: selection has tree shade, close to water source, the place of heat preservation, moisturizing, ventilation
Phellinus greenhouse is built, and carries out strict sterilization.The old mulberry tree pier for being inoculated with wild Phellinus bacterium solution is placed in greenhouse, temperature 27 is controlled
± 1 DEG C of degree, pays attention to sunshade moisturizing, expands under the conditions of dim light or scattering light numerous, and cloudy day or rainy day do not spray water, and natural rainwater waters,
Sunny day uses spray pattern to spray 6 water daily, 10 minutes every time, keeps humidity 80% or more.It expands to form cap when former base
When, Yao Zengjia spray timings improve humidity, keep humidity 90% or more, temperature 20-25 degree pays attention to ventilation, Phellinus fructification
It can be harvested when mature.
(6) pest management: Phellinus is easy the harm of fungal infection and virus and acarid after going out Huang, therefore generally exists
Phellinus goes out after Huang to picking, need to periodically sprinkle 75% alcohol and be prevented, 5% limewash or 0.15% hundred is sprayed after generation
Bacterium is prevented and treated clearly.
Embodiment 3:
(1) strain and cultural hypha are obtained: wild Phellinus strain acquisition and cultural hypha: by the mulberry tree for being 15 years from the age of tree
The wild Phellinus that upper acquisition obtains, carries out in vitro cultural hypha, cuts the rectangular fritter of 2.12cm × 0.87cm × 0.512cm,
After 75% ethyl alcohol surface sterilization, grinding carries out culture medium culture, culture medium prescription are as follows: 3 parts of ramulus mori liquid, 11 parts of glucose, di(2-ethylhexyl)phosphate
1.5 parts of hydrogen potassium, 0.6 part of magnesium sulfate, 4 parts of peptone, 4 parts of agar powder, 2 parts of streptomysin (50mg/L), 1000 parts of water, cultivation temperature
28 DEG C, culture humidity 38%, incubation time 3-5 days.The wherein preparation of ramulus mori liquid: winning 600 parts of new fresh mulberry leafs, is added 150 parts
Water is ground, filtering, and the centrifugation of 300r/min room temperature is made.
(2) prepared by Phellinus Inoculant: by the good phellinus liteus access culture medium of pure culture, it is small to carry out shaking table culture 48
When, the Phellinus bacterium solution of every milliliter of 106 spores is then made.
(3) inoculation is expanded numerous: by the upward 15 degree oblique cuttings of Phellinus bacterium solution inject 1ml to the age of tree for above 18 years old mulberry tree piers into
Row expands numerous；Old mulberry tree pier vertical display, there are gaps between old mulberry tree pier, are conducive to ventilation；Injection site should select below mulberry fist
Cortex portion, each mulberry tree pier be staggered inoculation 5 positions.
(4) expand numerous nutritional supplementation: after old mulberry tree pier inoculation Phellinus, within the scope of from injection point 8cm, 5ml is respectively adopted
Glass syringe, oblique cutting injects 22ml nutritional agents, is typically chosen in injection point two orientation injection up and down.Injection time is to connect
It is primary every injection in 15 days after kind Phellinus, until harvesting.Nutrition agent prescription are as follows: 11 parts of vitamin A, 26 parts of vitamin B, dimension
Raw 2.5 parts of element D .5 parts of amino acid 1,7 parts of active fructose, 14 parts of glucose, 28 parts of zinc sulfate, 36 parts of magnesium sulfate, melissane
1.5 parts of alcohol, 110 parts of urea, 3.5 parts of potassium dihydrogen phosphate, 1150 parts of water.
(5) numerous condition selection and management are expanded in bionical border: selection has tree shade, close to water source, the place of heat preservation, moisturizing, ventilation
Phellinus greenhouse is built, and carries out strict sterilization.The old mulberry tree pier for being inoculated with wild Phellinus bacterium solution is placed in greenhouse, temperature 25 is controlled
± 1 DEG C of degree, pays attention to sunshade moisturizing, expands under the conditions of dim light or scattering light numerous, and cloudy day or rainy day do not spray water, and natural rainwater waters,
Sunny day uses spray pattern to spray 5 water daily, 9 minutes every time, keeps humidity 80% or more.When former base expands to form cap,
Increase spray timings, improve humidity, keeps humidity 90% or more, temperature 20-25 degree pays attention to ventilation, and Phellinus fructification is mature
When can harvest.
(6) pest management: Phellinus is easy the harm of fungal infection and virus and acarid after going out Huang, therefore generally exists
Phellinus goes out after Huang to picking, need to periodically sprinkle 75% alcohol and be prevented, 5% limewash or 0.15% hundred is sprayed after generation
Bacterium is prevented and treated clearly.
Claims (1)
1. a kind of bionical border expanding propagation method of wild Phellinus, step:
(1) wild Phellinus strain acquisition and cultural hypha: the Wild Mulberry obtained will be acquired from the mulberry tree that the age of tree is 15 years or more
Huang carries out in vitro cultural hypha, cuts having a size of the rectangular fritter of 1.8-2.2cm × 0.9-1.3cm × 0.35-0.55cm, 75%
After ethyl alcohol surface sterilization, grinding carries out culture medium culture, culture medium prescription parts by weight are as follows: 2-3 parts of ramulus mori liquid, glucose 10-12
Part, 1-2 parts of potassium dihydrogen phosphate, 0.5-0.8 parts of magnesium sulfate, 4-6 parts of peptone, 3-5 parts of agar powder, 2-3 parts of 50mg/L streptomysin,
1000 parts of water, 28 DEG C of cultivation temperature, cultivate humidity 30-45%, incubation time 3-5 days；The wherein preparation of ramulus mori liquid: 500- is won
100-300 parts of water are added in 800 parts of new fresh mulberry leafs, grind, filtering, and the centrifugation of 300r/min room temperature is made；
(2) prepared by Phellinus Inoculant: the good phellinus liteus of pure culture being accessed in culture medium, progress shaking table culture 48 hours, so
The Phellinus bacterium solution of every milliliter of 106 spores is made afterwards；
(3) bionical border expand numerous select and management: the bionical border of Phellinus expands numerous and selects the age of tree for 15 years or more mulberry tree stumps,
Stump length is not less than 70cm, is erected on the sandy loam face of good water-retaining property, suitable shady place is advisable；Stump is directly put together,
Or it individually puts；
(4) inoculation is expanded numerous: before inoculation, mulberry tree pier is first carried out disinfection with pasteurized water, after disinfection 6 hours, by concentration obtained
For the spore bacterium solution of 106/milliliter, it is injected on old mulberry tree pier using the glass Syringe injector of 10mL in upward 15 degree of oblique cuttings
Face expand numerous；Injection site should select the cortex portion below mulberry fist, and each mulberry tree pier, which is staggered, is inoculated with 5-6 position, after injection
Using round special sterile PE protective film, injection site is pasted, when Phellinus former base protrusion, removes PE protective film；
(5) expand numerous nutritional supplementation: after old mulberry tree pier inoculation Phellinus, within the scope of from injection point 10cm, the glass of 5ml is respectively adopted
Glass syringe, oblique cutting inject 20-25ml nutritional agents, are typically chosen in injection point two orientation injections up and down；Injection time is to connect
It is primary every injection in 15 days after kind Phellinus, until harvesting；Nutritional agents formulation weight part are as follows: 10-12 parts of vitamin A, vitamin B
25-28 parts, 2-3 parts of vitamin D .5-2 parts of amino acid 1,5-8 parts of active fructose, 13-15 parts of glucose, zinc sulfate 20-30
Part, 35-38 parts of magnesium sulfate, 1-2 parts of triacontanol, 100-110 parts of urea, 3-4 parts of potassium dihydrogen phosphate, 1000-1200 parts of water；
(6) numerous condition management is expanded in bionical border: shady and cool wet place is preferably placed on after old mulberry tree pier inoculation Phellinus, general control is in temperature
24-28 DEG C of degree, 80% or more humidity；Cloudy day or rainy day do not spray water, and sunny day uses spray pattern to spray 4-5 water, each 8-10 daily
Minute；
(7) go out yellow management: when former base expands to form cap, Yao Zengjia spray timings improve humidity, keep humidity 90% with
On, 20-25 DEG C of temperature；It can be harvested when Phellinus fructification maturation.
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| CN109168958B (en) * | 2018-10-11 | 2020-11-24 | 安康学院 | Phellinus igniarius cultivation method |
| CN110999716A (en) * | 2019-12-03 | 2020-04-14 | 江苏惠发农业科技发展有限公司 | Pleurotus geesteranus cultivation method using wheat straw as main raw material |
| CN112205238A (en) * | 2020-10-27 | 2021-01-12 | 常州市金坛区食用菌协会 | Bionic cultivation technology of phellinus igniarius |
| CN114503874B (en) * | 2022-01-10 | 2023-04-25 | 南充市农业科学院 | Mulberry and phellinus linteus planting method |
Family Cites Families (17)
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| KR100838113B1 (en) * | 2007-05-17 | 2008-06-13 | 김경오 | Cultivation method of phellinus linteus including edible gold dust and its phellinus linteus |
| CN101130756B (en) * | 2007-07-23 | 2010-08-25 | 陕西省微生物研究所 | Isolated culture method of wild phellinus igniarius mushroom mycopremna |
| CN101485262B (en) * | 2009-02-19 | 2011-06-15 | 北京林业大学 | Artificial cultivation method of Phellinus linteus |
| KR20120132646A (en) * | 2011-05-24 | 2012-12-07 | 권기식 | Liquid cultivating method of Phellinus linteus increasing antioxidant and immunostimulating activity |
| CN103299887B (en) * | 2012-03-16 | 2014-11-26 | 何寒 | Method for preparing phellinus igniarius hypha blocks by utilizing sprouted rice |
| CN103299824B (en) * | 2012-03-16 | 2014-12-24 | 何寒 | Method for preparing phellinus igniarius sclertium from fresh, tender and sweet corn |
| CN102786333B (en) * | 2012-06-21 | 2013-11-06 | 杭州清正生物科技有限公司 | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same |
| CN102875225A (en) * | 2012-09-20 | 2013-01-16 | 福建农林大学 | Phellinus igniarius bacterial strain liquid fermenting culture medium and method for fermenting and producing phellinus linteus polysaccharides |
| CN103340093A (en) * | 2013-06-24 | 2013-10-09 | 陕西省微生物研究所 | Artificial cultivation method for phellinus linteus |
| CN103493685A (en) * | 2013-10-14 | 2014-01-08 | 贵州省施秉县民生天麻科技有限公司 | Method for cultivating phellinus igniarius by using wild living tree stumps |
| CN103598010B (en) * | 2013-11-12 | 2015-04-29 | 淳安县微生物研究所 | Original ecological imitative wild cultivation method for inonotus sanghuang |
| CN105165404B (en) * | 2015-09-30 | 2017-08-22 | 金寨尚臻生物科技有限公司 | A kind of method for shortening the Phellinus sporophore growth cycle |
| CN105724049A (en) * | 2016-01-27 | 2016-07-06 | 浙江省农业科学院 | Mulberry parasitical phellinus strain wild environment returning rejuvenation culture method |
| CN105993593A (en) * | 2016-05-23 | 2016-10-12 | 浙江省农业科学院 | High-efficiency industrial bag material cultivation process of inonotus linteus with antitumor activity |
| CN106576910B (en) * | 2016-12-14 | 2020-04-21 | 山西省农业科学院食用菌研究所 | Culture method of in-vitro sterile host plant-mycorrhizal edible fungus symbiotic seedling |
| CN106941928A (en) * | 2017-03-02 | 2017-07-14 | 江苏道诚生物科技有限公司 | A kind of implantation methods of Phellinus |
| CN107058128B (en) * | 2017-03-30 | 2020-10-16 | 山东省菇园食用菌科技有限公司 | Phellinus igniarius strain and application thereof |
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2018
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