CN105660183A - Phellinus igniarius cultivation method - Google Patents

Phellinus igniarius cultivation method Download PDF

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Publication number
CN105660183A
CN105660183A CN201610059826.4A CN201610059826A CN105660183A CN 105660183 A CN105660183 A CN 105660183A CN 201610059826 A CN201610059826 A CN 201610059826A CN 105660183 A CN105660183 A CN 105660183A
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CN
China
Prior art keywords
phellinus igniarius
juggle
booth
quel
greenhouse
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Pending
Application number
CN201610059826.4A
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Chinese (zh)
Inventor
杨贵成
杨健东
王丽敏
孙德忠
孙利
周旭昌
王威利
杨建奇
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Jilin Sansheng Agriculture Development Group Co Ltd
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Jilin Sansheng Agriculture Development Group Co Ltd
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Priority to CN201610059826.4A priority Critical patent/CN105660183A/en
Publication of CN105660183A publication Critical patent/CN105660183A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention relates to fungi plant cultivation, in particular to a phellinus igniarius cultivation method. The method comprises the following steps: (1) making a plurality of cultivation beds in a greenhouse, paving fine sand with the thickness of 3cm, arranging a wood section with mature mycelia at an interval of 15cm, and cutting an opening with the width of 2mm and the length of 7cm in two sides of the 3cm part of the upper end of the wood section; (2) arranging an iron net around the greenhouse, paving a sunshade net on the iron net, and paving a layer of sunshade net and a layer of antioxidant film on the greenhouse; and (3) controlling all wood sections subjected to mycelium growth and spread to go to the field and enter the greenhouse for growth at the time from April 24 to May 10; for the wood sections entering the greenhouse, winding a plastic film at the upper part of the greenhouse on one side for later use on June 10, performing watering in the greenhouse by natural rain without water on a cloudy or rainy day, and covering the greenhouse with the plastic film wound on one side on a frost descent day, wherein the watering is performed for 4-5 times each day and for 5-7 minutes each time. The phellinus igniarius cultivation imitates wild growth, so that no pollution and no impurities are caused and the content of various components of phellinus igniarius is higher than that of phellinus igniarius growing on wild mulberry.

Description

Phellinus igniarius (L. ex Fr.) Quel. cultural method
Technical field
The present invention relates to a kind of fungi plant cultivation, i.e. Phellinus igniarius (L. ex Fr.) Quel. cultural method. Trade name: Changbai Mountain Phellinus igniarius (L. ex Fr.) Quel..
Background technology
In the prior art, Phellinus igniarius (L. ex Fr.) Quel. is medicinal fungi rare in the Nature, and " property of medicine opinion " in the Tang Dynasty is just on the books. Compendium of Material Medica is also on the books. Phellinus igniarius (L. ex Fr.) Quel. can control hypochondrium fluid retention gather, incised wound of suffering from abdominal pain. " Chinese medicine voluminous dictionary " describes it can treat the multiple disease of internal medicine, and its sporophore is used as medicine, mildly bitter flavor, energy profit the five internal organs, a surname intestinal gas, hemostasis, toxin expelling stomach function regulating antidiarrheal. Modern medicine study shows; Phellinus igniarius (L. ex Fr.) Quel. is except having the pharmacological action in Traditional Chinese medical theory; also there is the protection of anticancer, antitumor, defying age, resisting fatigue, antioxidation, fibrosis, hemopoietic function of bone marrow damage, reconcile immunologic function, protect the liver and the effect such as anti-liver cirrhosis.
Due to the restriction by the particularity of physiological ecological and the complexity of nature and environmental condition thereof, Phellinus igniarius (L. ex Fr.) Quel. is very rare in growth naturally, accordingly, it would be desirable to by substantial amounts of effort and exploration, seek a kind of artificial implantation method of the best.
Summary of the invention
It is an object of the invention to provide for above-mentioned deficiency a kind of imitative wild, illumination rationally, the Phellinus igniarius (L. ex Fr.) Quel. cultural method of quality better.
The technical solution of the present invention is: a kind of Phellinus igniarius (L. ex Fr.) Quel. cultural method, and its step is as follows:
(1) select pollution-free, booth is set up in the area that water source permits point, makees cultivation bed a plurality of in booth, middle bedside, both sides bed is narrow, the fine sand that paving 3cm is thick, puts, every 15 centimeters, the juggle that a mycelia is ripe, and the mouth of 2 mm wides, 7cm length is cut in the both sides measuring 3cm place under juggle upper end, control sporophore from then on mouth to bear, juggle occupies cultivation bed, trains 3cm thickness with fine sand, with water conservation part bottom juggle.
(2) fix at booth surrounding 4 × 4 angle steel overhead 30cm place; longitudinal direction iron wire; horizontal iron wire stretches respectively and is fixed on angle steel; forming ferrum net, then spread one layer of sunshade net one layer on the net at ferrum, surrounding binds fixing; micro-sparge pipe is arranged online; with anti-ageing film one layer on canopy, sunshade net one layer, at this moment a complete Phellinus igniarius (L. ex Fr.) Quel. growth booth completes.
(3) booth management: the juggle sending out well bacterium controls all to enter down booth between April 20 to May 10 and grows; Enter the juggle of booth; early stage to be ventilated less, it is ensured that certain humidity and temperature, is placed on side to the plastic film rolls booth top on the June 10 stand-by; do not water in cloudy day or rainy day booth; watering with the rain of nature, sunny day waters 4-5 water every day, each 5-7 minute; the plastic foil that the same day in the Frost's Descent is placed on side volume again covers again; later normal management, winter overwintering, Phellinus igniarius (L. ex Fr.) Quel. is in a dormant state.
In such scheme:
Booth is wide 6.5 meters, long 28 meters, makees cultivation bed three in booth, and 2000 juggles occupy three husky beds.
Described ferrum net is longitudinally with four 8#Iron wire, horizontal use 12 8#Iron wire stretches respectively and is fixed on angle steel.
The juggle of described mycelia maturation is that Xylosmae japonici or Kuwaki are split into some fritters, forms diameter 15cm, and the juggle of high 18cm, sterilizing is standby.
Juggle after subject to sterilization, when temperature drops to 20 DEG C, carries out connecing bacterium by sterile working's code, adopts two ends inoculation, complete for juggle section lid.
The juggle connecing bacterium send culturing room to cultivate, and the temperature of culturing room must control at 28 DEG C-30 DEG C for first 15 days, between humidity 80-95, give the scattering light of 15-20%, on the basis of open ventilation fan, to ventilate every day 2 times, each 1 hour, juggle incubation time was at 50-60 days.
Phellinus igniarius (L. ex Fr.) Quel. mother culture media is made up of following raw material: glucose 20g, soy peptone 2g, agar 18g, potassium dihydrogen phosphate 0.46g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, water 1000ml.
Phellinus igniarius (L. ex Fr.) Quel. pedigree seed culture medium is made up of following raw material: Kuwaki, Xylosmae japonici mixing wood flour 75%, Testa Tritici 20%, analysis for soybean powder 2%, glucose 2%, Gypsum Fibrosum 1%.
The invention have the advantage that 1, Phellinus igniarius (L. ex Fr.) Quel. cultivation back to nature, wild growth of walking back and forth, receive the baptism of the Nature, maintain the active ingredient of Phellinus igniarius (L. ex Fr.) Quel. sporophore to the full extent. 2, pollution-free, free from admixture, content is abundant, pure green. 3, color and luster is beautiful, sense organ is good, yield is high, and average each juggle can produce Phellinus igniarius (L. ex Fr.) Quel. 125-150g. 4, the content of its various compositions of Phellinus igniarius (L. ex Fr.) Quel. grown with above method management after testing is higher than the various content of Phellinus igniarius (L. ex Fr.) Quel. of wild Kuwaki growth.
Below in conjunction with embodiment, embodiments of the present invention are described in further detail.
Detailed description of the invention
Phellinus igniarius (L. ex Fr.) Quel. cultural method:
1, mother culture media is made up of following raw material: glucose 20g, soy peptone 2g, agar 18g, potassium dihydrogen phosphate 0.46g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, water 1000ml.
Female source of planting obtains in July, 2005 in the virgin forest of master ridge, Changbai Mountain; relatively take Phellinus igniarius (L. ex Fr.) Quel. mushroom block conventional method fresh, that type is beautiful, healthy and strong to separate; it is placed in 28 DEG C of 30 DEG C of incubators and cultivates; when sporophore periphery grows milk yellow mycelia; select the most healthy and strong local picking mycelium to be transplanted in new test tube stock culture medium in mycelia one end; as the method is transplanted twice, reaching detoxification purpose, obtained strains is planted standby as mother. Thus get Changbai Mountain Phellinus igniarius (L. ex Fr.) Quel..
2, Phellinus igniarius (L. ex Fr.) Quel. pedigree seed culture medium is made up of following raw material: Kuwaki, Xylosmae japonici mixing wood flour 75%, Testa Tritici 20%, analysis for soybean powder 2%, glucose 2%, Gypsum Fibrosum 1%. Kuwaki: Xylosmae japonici=3:7.
Bacterium rod made by the Primary spawn bacterium bag of 33 × 15cm, when high temperature sterilize temperature lowers, connect female kind, connect in Mu Zhonghousong culturing room and cultivated, culturing room to shift to an earlier date sterilizing in three days, twice daily, in time determining culturing room without miscellaneous bacteria, then the bacterium rod connected is sent into indoor cultivation, cultivates indoor temperature therebetween and to be maintained at 28 DEG C 30 DEG C, between humidity 76-90, adopting ventilation fan to adjust space humiture all permanent, indoor give the scattering light of 10% 15%, and light can not be too strong, keep the ventilation of 2 short time every day, exchange fresh oxygen for. The Best Times that strain is ripe, is typically in 38-45 days.
3, juggle planting material composition: adopt Xylosmae japonici or Kuwaki without exception, juggle is split into some fritters, forms the bacterium section of diameter 15cm, high 18cm, in the plastic bag of loading, standby after high temperature sterilize.
Juggle after subject to sterilization, when temperature drops to about 20 DEG C, carries out connecing bacterium by sterile working's code, and strain selects the cultigen that mycelial growth is vigorous, adopts two ends inoculation, and order ground strengthens mycelial growth area, effectively controls other miscellaneous bacteria infection chance. Inoculum concentration is generally best juggle section lid entirely.
The juggle connecing bacterium send culturing room to cultivate, and the temperature of culturing room must control, at 28 DEG C 30 DEG C, between humidity 80 95, to give the scattering light of 15-20% for first 15 days, on the basis of open ventilation fan, to ventilate every day 2 times, each 1 hours. Guarantee the supply of fresh oxygen. Juggle incubation time is not less than 50-60 days, and otherwise mycelia eats thoroughly not immature.
4, cultural method
(1) selecting pollution-free, booth is set up in the area that water source permits point, and booth length is wide 6.5 meters; long 28 meters; doing cultivation bed three, middle bedside in booth, both sides bed is narrow; the fine sand that paving 3cm is thick; putting, every 15 centimeters, the juggle that mycelia is ripe, measure the both sides at 3cm place and cut 2 millimeters, the mouth of 7cm length under juggle upper end, control sporophore from then on mouth bears; this ensure that Phellinus igniarius (L. ex Fr.) Quel. is not subject to the pollution of silt in growth course, it is ensured that content of beary metal does not exceed standard. 2000 juggles occupy three husky beds, train 3cm thickness with fine sand, water conservation part bottom juggle.
(2) fix at booth surrounding 4 × 4 angle steel overhead 30cm place, vertical with four 8#Iron wire, horizontal use 12 8#Iron wire stretches respectively and is fixed on angle steel, material is thus formed a ferrum net, then spreads one layer of knitting sunshade net of specification four one layer on the net at ferrum, and surrounding binds fixing, micro-sparge pipe on the cloth of online appropriate location. With anti-ageing film one layer on canopy, sunshade net one layer, at this moment a complete Phellinus igniarius (L. ex Fr.) Quel. growth booth completes.
5, booth management
By cultivation experience for many years, summing up following booth juggle growth management method, the juggle general control sending out bacterium good all enters down booth between solar calendar April 20 to May 10 and grows. Entering the juggle of booth, early stage to be ventilated less, it is ensured that certain humidity and temperature; promote phellinus liteus fast-growth, be placed on side to the solar calendar plastic film rolls booth top on the June 10 stand-by, cloudy day or do not water in rainy day booth; water with the rain of nature; sunny day waters 4-5 water every day, each 5-7 minute, and the same day in the Frost's Descent is covering at plastic foil volume being placed on side; later normal management; winter survives the winter naturally, and Phellinus igniarius (L. ex Fr.) Quel. in a dormant state, can gather by three whole year.
Being described above, simply the specific embodiment of the present invention, the flesh and blood of the present invention is not construed as limiting by various illustrations.

Claims (6)

1. a Phellinus igniarius (L. ex Fr.) Quel. cultural method, it is characterised in that step is as follows:
(1) select pollution-free, booth is set up in the area that water source permits point, makees cultivation bed a plurality of in booth, middle bedside, both sides bed is narrow, the fine sand that paving 3cm is thick, puts, every 15 centimeters, the juggle that a mycelia is ripe, and the mouth of 2 mm wides, 7cm length is cut in the both sides measuring 3cm place under juggle upper end, control sporophore from then on mouth to bear, juggle occupies cultivation bed, trains 3cm thickness with fine sand, with water conservation part bottom juggle;
(2) fix at booth surrounding 4 × 4 angle steel overhead 30cm place, longitudinal direction iron wire, horizontal iron wire stretches respectively and is fixed on angle steel, forming ferrum net, then spread one layer of sunshade net one layer on the net at ferrum, surrounding binds fixing, micro-sparge pipe is arranged online, with anti-ageing film one layer on canopy, sunshade net one layer, at this moment a complete Phellinus igniarius (L. ex Fr.) Quel. growth booth completes;
(3) booth management: the juggle sending out well bacterium controls all to enter down booth between April 20 to May 10 and grows; Enter the juggle of booth; early stage to be ventilated less, it is ensured that certain humidity and temperature, is placed on side to the plastic film rolls booth top on the June 10 stand-by; do not water in cloudy day or rainy day booth; watering with the rain of nature, sunny day waters 4-5 water every day, each 5-7 minute; the plastic foil that the same day in the Frost's Descent is placed on side volume again covers again; later normal management, winter overwintering, Phellinus igniarius (L. ex Fr.) Quel. is in a dormant state.
2. the Phellinus igniarius (L. ex Fr.) Quel. cultural method described in claim 1, it is characterised in that booth is wide 6.5 meters, makees cultivation bed three by long 28 meters in booth, 2000 juggles occupy three husky beds.
3. the Phellinus igniarius (L. ex Fr.) Quel. cultural method described in claim 1 or 2, it is characterised in that described ferrum net is longitudinally with four 8#Iron wire, horizontal use 12 8#Iron wire stretches respectively and is fixed on angle steel.
4. the Phellinus igniarius (L. ex Fr.) Quel. cultural method described in claim 1, it is characterised in that the juggle of described mycelia maturation is that Xylosmae japonici or Kuwaki are split into some fritters, forms diameter 15cm, the juggle of high 18cm, and sterilizing is standby;
Juggle after subject to sterilization, when temperature drops to 20 DEG C, carries out connecing bacterium by sterile working's code, adopts two ends inoculation, complete for juggle section lid;
The juggle connecing bacterium send culturing room to cultivate, and the temperature of culturing room must control at 28 DEG C 30 DEG C for first 15 days, between humidity 80 95, give the scattering light of 15-20%, on the basis of open ventilation fan, to ventilate every day 2 times, each 1 hour, juggle incubation time was at 50-60 days.
5. the Phellinus igniarius (L. ex Fr.) Quel. cultural method described in claim 1, it is characterised in that Phellinus igniarius (L. ex Fr.) Quel. mother culture media is made up of following raw material: glucose 20g, soy peptone 2g, agar 18g, potassium dihydrogen phosphate 0.46g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, water 1000ml.
6. the Phellinus igniarius (L. ex Fr.) Quel. cultural method described in claim 1, it is characterised in that Phellinus igniarius (L. ex Fr.) Quel. pedigree seed culture medium is made up of following raw material: Kuwaki, Xylosmae japonici mixing wood flour 75%, Testa Tritici 20%, analysis for soybean powder 2%, glucose 2%, Gypsum Fibrosum 1%.
CN201610059826.4A 2016-01-29 2016-01-29 Phellinus igniarius cultivation method Pending CN105660183A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107021816A (en) * 2017-04-21 2017-08-08 张媛 A kind of cultural method for improving Phellinus polyoses content
CN107646542A (en) * 2017-11-02 2018-02-02 成都金川田农机制造有限公司 Phellinus cultural method
CN107996288A (en) * 2017-12-29 2018-05-08 浙江省林业科学研究院 A kind of method that hayashishita imitates Wild ecological cultivation Phellinus
CN108739056A (en) * 2018-04-18 2018-11-06 浙江佐力药业股份有限公司 Application of the wheatfeed in inhibiting Phellinus fermentation mycelium aging
CN109220526A (en) * 2018-10-12 2019-01-18 绩溪县徽菜宝生物科技有限公司 A kind of Phellinus large-scale planting method
CN109348997A (en) * 2018-12-14 2019-02-19 信阳市菌福康生物科技有限公司 Phellinus strain is planted and artificial cultivation method
CN111296172A (en) * 2020-03-26 2020-06-19 安徽省森湶谷药业股份有限公司 Method for cultivating phellinus igniarius in double-bag connected stacked rows

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101485262A (en) * 2009-02-19 2009-07-22 北京林业大学 Artificial cultivation method of Phellinus linteus
CN101816256A (en) * 2009-02-27 2010-09-01 上海市农业科学院 Method for cultivating microbial strain of phellinus linteus and planting phellinus linteus
CN102523909A (en) * 2010-12-28 2012-07-04 秦绍新 Method for planting phellinus igniarius by utilizing imitation cut-logs of mulberry twigs
CN103340093A (en) * 2013-06-24 2013-10-09 陕西省微生物研究所 Artificial cultivation method for phellinus linteus
CN103804054A (en) * 2014-02-20 2014-05-21 通化长白山药谷集团有限公司 Culture medium optimizing and short cut log cultivating method of phellinus igniarius

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101485262A (en) * 2009-02-19 2009-07-22 北京林业大学 Artificial cultivation method of Phellinus linteus
CN101816256A (en) * 2009-02-27 2010-09-01 上海市农业科学院 Method for cultivating microbial strain of phellinus linteus and planting phellinus linteus
CN102523909A (en) * 2010-12-28 2012-07-04 秦绍新 Method for planting phellinus igniarius by utilizing imitation cut-logs of mulberry twigs
CN103340093A (en) * 2013-06-24 2013-10-09 陕西省微生物研究所 Artificial cultivation method for phellinus linteus
CN103804054A (en) * 2014-02-20 2014-05-21 通化长白山药谷集团有限公司 Culture medium optimizing and short cut log cultivating method of phellinus igniarius

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107021816A (en) * 2017-04-21 2017-08-08 张媛 A kind of cultural method for improving Phellinus polyoses content
CN107646542A (en) * 2017-11-02 2018-02-02 成都金川田农机制造有限公司 Phellinus cultural method
CN107996288A (en) * 2017-12-29 2018-05-08 浙江省林业科学研究院 A kind of method that hayashishita imitates Wild ecological cultivation Phellinus
CN107996288B (en) * 2017-12-29 2020-11-10 浙江省林业科学研究院 Method for ecologically cultivating phellinus igniarius under forest by imitating wild conditions
CN108739056A (en) * 2018-04-18 2018-11-06 浙江佐力药业股份有限公司 Application of the wheatfeed in inhibiting Phellinus fermentation mycelium aging
CN109220526A (en) * 2018-10-12 2019-01-18 绩溪县徽菜宝生物科技有限公司 A kind of Phellinus large-scale planting method
CN109348997A (en) * 2018-12-14 2019-02-19 信阳市菌福康生物科技有限公司 Phellinus strain is planted and artificial cultivation method
CN111296172A (en) * 2020-03-26 2020-06-19 安徽省森湶谷药业股份有限公司 Method for cultivating phellinus igniarius in double-bag connected stacked rows

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Application publication date: 20160615