CN107021816A - A kind of cultural method for improving Phellinus polyoses content - Google Patents
A kind of cultural method for improving Phellinus polyoses content Download PDFInfo
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- CN107021816A CN107021816A CN201710265348.7A CN201710265348A CN107021816A CN 107021816 A CN107021816 A CN 107021816A CN 201710265348 A CN201710265348 A CN 201710265348A CN 107021816 A CN107021816 A CN 107021816A
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- phellinus
- substrate material
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- growth regulator
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of cultural method for improving Phellinus polyoses content, prepared including substrate material, pack, high-temperature sterilization, inoculation, cultural hypha, crop field management of producing mushroom and harvesting step, prepared in the substrate material, plant growth regulator is added in inoculation or any one step or any two step in cultural hypha step, plant growth regulator is by oleic acid, any one or two kinds in αnaphthylacetate or 3 indolebutyric acids are combined, the technical program discloses a kind of cultural method for improving Phellinus polyoses content, the value of Phellinus can not only be improved, it is also to increase the effective way of mushroom grower income, also have operating technology simple, it is easy to operate, cost is relatively low, the characteristics of resource can make full use of.
Description
Technical field
The present invention relates to a kind of cultural method of edible mushroom, and in particular to a kind of cultivation side of raising Phellinus polyoses content
Method.
Background technology
Phellinus alias monkey eye, mulberry ear, phellinus, Sang Chen etc., are a kind of fungies, are gained the name because parasitizing mulberry tree.Mulberry
Yellow ancients are referred to as " artist's conk ", generally parasitize the rotten part of the trunks such as mulberry tree, pine tree, willow, birch, oak.Heartwood white rot is caused,
It is perennial, although the fungi colonized on trees is referred to alternatively as artist's conk, but the artist's conk only parasitized on mulberry tree is only orthodox school
Phellinus, and it is other then can only as Phellinus substitute, and drug effect and Phellinus are different.
According to《Haigoushen》Record：Phellinus it is sweet it is flat, nontoxic, control metrorrhagia, blood strangury, prolapse of the anus rush down blood, band under, amenorrhoea.Fructification
Stockless, the flat hemispherical of cap or the shape of a hoof, it is wooden, it is shallow liver brown to dark gray or black.On the trunks such as poplar, willow, birch, oak
There is growth.Be distributed the ground such as Northeast China, North China, northwest and Sichuan, Yunnan, Phellinus is a kind of rare Chinese medicine, can relieving the five internal organs, it is soft
Heavily fortified point, toxin expelling stops blooding, promoting blood circulation, and stomach antidiarrheal.Cure mainly under gonorrhoea, uterine bleeding band, a lump in the abdomen causing distension and pain, addiction drink, splenasthenic diarrhea.In recent years, with
Phellinus has the report of antitumor activity, and the consumption of Phellinus is increasingly increased, and particularly Korea Spro, day robs formula to China's wild resource
Purchase, the deposit of wild Phellinus resource is fewer and fewer.As people's health care consciousness increasingly strengthens, Phellinus anti-tumor health care product will
Obtain the favor of consumers in general.
Modern medicine and nutrition deepen continuously research, also containing abundant polysaccharide component in Phellinus, in Phellinus fructification
Polyoses content it is high, medical value is very high.The polyoses content of Phellinus fructification is improved in Phellinus plantation, Phellinus is just improved
The quality of entity.Meet demand of the medicine to Phellinus.
Produced according to existing artificial cultivation Phellinus：It is substrate material preparation, pack, high-temperature sterilization, inoculation, cultural hypha, big
Field management of producing mushroom, harvesting technological process；
Substrate material is prepared：Major ingredient and auxiliary material are accurately weighed up by formula rate, is stirred, is added water in right amount, it is suitable to reach
Material-water ratio, then carries out pouch step；
Pack：It is that the cultivation Phellinus substrate material that will have been configured is fitted into film special cylinder, cultivation Phellinus cultivation is made in sealing
Rod；
High-temperature sterilization：It is that Phellinus is cultivated into rod, is placed on sterilizing chamber and is sterilized, sterilising temp keeps 10~12 up to 100 DEG C
Hour, cooled down after sterilizing, be transferred to inoculation process；
Inoculation：It can be inoculated with when bacterium rod temperature is naturally cooled to below 28 DEG C, bacterium rod is wanted to be inoculated with when burrowing, with a collection of
Bacterium rod will be inoculated with batch and complete.Bacterium rod after inoculation is inserted the vertically and horizontally arranged stacking in bacterium germination room, then lid film.One week
Afterwards, daily early morning will be aerated ventilation once, one hour or so time；
Cultural hypha：
Referred to as bacterium germination management during inoculation terminates to bacterium rod opening,
1st, mycelia is colonized.In about 7~15 days this stages, this stage should not move bacterium rod, and temperature is in the range of 22-30 DEG C.
2nd, check that miscellaneous bacteria is deflated.After mycelia field planting, 4 centimetres to 5 centimetres of mycelia loop diameter carries out first time turning acanthopore and put
Miscellaneous bacteria gas oxygenating gas, acanthopore 15-20 is advisable；Treat that mycelia all covers with second of deflation of progress, acanthopore is advisable at 35-45.
Temperature during deflation is maintained between 25-32 DEG C, and bacterium rod stacks triangularity after deflation, is highly advisable no more than ten layers.
3rd, bacterium rod is covered with after deflation, also wants antiforeign bacteria to pollute, discovery will isolate in time, interior will divulge information, it is impossible to allow sunlight
Irradiation, temperature is maintained at 25-32 DEG C.
Bacterium rod opening：See that mycelia volume expansion around bag wall, gauffer, protuberance knob occur, you can bacterium rod opening.
Management of producing mushroom：According to Phellinus bacterium rod conventional technique standard operation.
Harvesting：Treat that cap is fully grown up, harvested when switching to grey black by yellow.
The present invention, can not only on the basis of existing technology there is provided a kind of square cultural method for improving Phellinus polyoses content
The value of Phellinus is improved, is also to increase the effective way of mushroom grower income, it is of far-reaching significance.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of cultivation side for improving Phellinus polyoses content
Method, relatively low to solve Phellinus polyoses content of the prior art, economic value is not high, and planting type is complicated, and culture and utility rate is low
Technical problem；
The present invention is achieved by the following technical solutions：The invention discloses a kind of cultivation for improving Phellinus polyoses content
Method, including substrate material preparation, pack, high-temperature sterilization, inoculation, cultural hypha, management of producing mushroom and harvesting step, wherein, in base
Addition biological growth regulation in material preparation, inoculation or any one step or any two step in cultural hypha step
Agent, plant growth regulator is used cooperatively by any one in oleic acid, α-naphthylacetic acid or 3- indolebutyric acids or any two kinds；
Further, in substrate material preparation steps, the component (weight) of substrate material is：
Wherein, the moisture content in substrate material is 60~65%, while adding plant growth regulator 0.1 in substrate material
~0.5 part；Accurately weighed up after major ingredient and auxiliary material by formula rate, stir, add water in right amount repeatedly, reach suitable material water
Than then carrying out pouch step；
Further,, before inoculation step starts after high-temperature sterilization step terminates in order to be preferably inoculated with
It is 0.05~0.1 part of plant growth regulator that constituent content is first injected at inoculation mouth, and inoculation step is then carried out again, wherein,
Not influence inoculation, the depth of plant growth regulator injection is 2/3 position of bacterium rod thickness.
Further, required according to the operating technology in bacterium rod cultural hypha stage, in the cultural hypha after mushroom rod inoculation, when
When bacterium rod mycelia is covered with substantially, miscellaneous bacteria gas oxygenating gas is put to bacterium rod acanthopore, miscellaneous bacteria gas oxygenating gas process is put using bacterium rod acanthopore
To improve lentinan content, and toward injection constituent content in bacterium rod be 0.05~0.1 part of plant growth regulator, wherein,
Plant growth regulator injection depth is at the 1/2 of bacterium rod thickness.
Further, Phellinus polyoses content in order to better improve, prepares and is inoculated with or cultural hypha step in substrate material
In be separately added into plant growth regulator, as a result prove, the polyoses content that this planting technique is obtained is more preferably.
The invention discloses a kind of cultural method for carrying Phellinus polyoses content, according in the different cultivation stages to Phellinus bacterium
The plant growth regulator of proper content kind is added, the content of polysaccharide is substantially increased in Phellinus body and with operation letter
The characteristics of single, utilization rate is high, planting type is simple, Phellinus economic value is high.Using the adjustment effect of plant growth regulator, carry
The high bioconversion in mycelial cultivation stage, is easy to effectively utilize for mycelia in mycelial culture, promotes mycelia
The polysaccharide conversion of raw body, shortens the Mycelium culture time, improves production efficiency.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementations
Example.
Embodiment 1
Embodiment 1 discloses a kind of cultural method for improving Phellinus polyoses content, including substrate material is prepared, packed, high temperature
Sterilizing, inoculation, cultural hypha, management of producing mushroom and harvesting step,
Substrate material is prepared：Major ingredient and auxiliary material are accurately weighed up by formula rate, is stirred, is added water in right amount, it is suitable to reach
Material-water ratio, then carries out pouch step；Pack：It is that the cultivation Phellinus substrate material that will have been configured is fitted into film special cylinder, sealing
Mushroom culture cultivation rod is made；High-temperature sterilization：It is that Phellinus is cultivated into rod, is placed on sterilizing chamber and is sterilized, sterilising temp is up to 100
DEG C, kept for 10~12 hours, cooled down after sterilizing, be transferred to inoculation process；Inoculation：When bacterium rod temperature is naturally cooled to below 28 DEG C
It can be inoculated with, bacterium rod is wanted to be inoculated with when burrowing, to be inoculated with and complete with batch with a collection of bacterium rod.Bacterium rod after inoculation is inserted bacterium germination room
It is vertically and horizontally arranged to stack, then lid film.After one week, daily early morning will be aerated ventilation once, one hour or so time；
Cultural hypha：Referred to as bacterium germination management during inoculation terminates to take off bag to bacterium rod；Including mycelia field planting, this stage about 7
~15 days, this stage should not move bacterium rod, and temperature is in the range of 22-30 DEG C；Check that miscellaneous bacteria is deflated, after mycelia field planting, mycelia circle
4 centimetres to 5 centimetres of diameter, carries out first time turning acanthopore and puts miscellaneous bacteria gas oxygenating gas, acanthopore 15-20 is advisable；Treat that mycelia is whole
Second of deflation of progress is covered with, acanthopore is advisable at 35-45.Temperature during deflation is maintained between 25-32 DEG C, bacterium rod after deflation
Triangularity is stacked, is highly advisable no more than ten layers；Bacterium rod is covered with after deflation, also wants antiforeign bacteria to pollute, and discovery will be isolated in time,
Interior will divulge information, it is impossible to allow sunlight to irradiate, and temperature is maintained at 25-32 DEG C.De- bag：See mycelia volume expansion around bag wall, it is gauffer, grand
Play knob to occur, you can bacterium rod opening, management of producing mushroom：According to Phellinus bacterium rod conventional technique standard operation.Harvesting：Treat cap
Fully grow up, harvested when switching to grey black by yellow.
The present embodiment only adds plant growth regulator in substrate material process for preparation, and plant growth regulator is oleic acid,
In substrate material preparation steps, the component (weight) of substrate material is：70 parts of ramulus mori wood chips, 5 parts of wheat bran, 0.5 part of glucose, 1 part of life
Lime, 1 part of calcium superphosphate, moisture content are 60~65%；0.1 part of oleic acid of auxiliary material is added in substrate material simultaneously, after stirring
Pouch step is carried out again.Polyoses content in Phellinus fructification, can improve 15% or so.
Embodiment 2
Embodiment 2 is identical with the operating procedure in embodiment 1, and difference is, in substrate material preparation, inoculation in embodiment 2
During add plant growth regulator, plant growth regulator is the base in oleic acid and α-naphthylacetic acid, substrate material preparation steps
The component (weight) of material is：83 parts of ramulus mori wood chip；12 parts of wheat bran；1 part of glucose, 2 parts of quick lime, 1.5 parts of calcium superphosphate, contain
Water rate is 60~65%.0.2 part of oleic acid is added in substrate material simultaneously, inoculation step starts after high-temperature sterilization step terminates
Before, constituent content is first injected at inoculation mouth is 0.03 part of α-naphthylacetic acid, and inoculation step is then carried out again, wherein, it is biological
The depth of growth regulator injection is 2/3 position of bacterium rod thickness.Polyoses content in Phellinus fructification, can improve 20%.
Embodiment 3
Embodiment 3 is identical with the cultivation step taken in embodiment 1, and difference is, is prepared in the present embodiment in substrate material
With all additions plant growth regulator in cultural hypha step, plant growth regulator is by oleic acid and 3- indolebutyric acids；
In substrate material preparation steps, the component (weight) of substrate material is：85 parts of ramulus mori wood chip；12 parts of wheat bran；1.5 parts of glucose, raw stone
1.5 parts of ash, 1 part of calcium superphosphate, moisture content are 60~65%.0.05 part of oleic acid is added in substrate material simultaneously；By recipe ratio
Example is accurately weighed up after major ingredient and auxiliary material, is stirred repeatedly, is then carried out pouch step；It is to treat bacterium in cultural hypha step
Rod mycelia is covered with when handling bacterium rod acanthopore, and it is 0.03 part of α-naphthylacetic acid that constituent content is injected into bacterium rod, wherein, biology is raw
Long conditioning agent injection depth is at the 1/2 of bacterium rod thickness.Polyoses content in Phellinus fructification, can improve more than 25%.
Embodiment 4
Embodiment 4 discloses a kind of process of conventional Phellinus cultivation in the cultural hypha stage, and substrate material is matched somebody with somebody, ramulus mori wood
85 parts of bits；12 parts of wheat bran；1.5 parts of glucose, 1.5 parts of quick lime, 1 part of calcium superphosphate, moisture content are 60~65% substrate material
In, add any one in 0.1-0.5 parts of α-naphthylacetic acids of components by weight percent or 3- indolebutyric acids；
This method technique is simple, and plant growth regulator activity is reduced in high-temperature sterilization process, and consumption is big, mulberry
Polyoses content in yellow fructification, can improve 10-15%.
Embodiment 5
With reference to embodiment 4,3- indolebutyric acids are added in inoculation step, specific operating method is：In inoculation mouth before inoculation
Place, according to bacterium rod weight, is inoculated after 0.05-0.1 parts of plant growth regulators of injection, is not influence inoculation, injection depth will
At reach bacterium rod thickness 2/3.
Although this method technique is simple, for artificial operation, a procedure is added, recruitment cost is improved, right
A set of liquid injecting mechanism can be added in mechanical inoculation device for mechanically actuated, improvement cost is not high, in Phellinus fructification
Polyoses content, can improve 15-20%.
Embodiment 6
Oleic acid is added in the bacterium rod cultural hypha stage in the present embodiment, specific operating method is：Trained according to bacterium rod mycelia
In the operating technology requirement in the stage of supporting, the cultural hypha after mushroom rod inoculation, when mycelia loop diameter reaches 4-5cm, first is carried out
When secondary turning acanthopore puts miscellaneous bacteria gas oxygenating gas to bacterium rod, according to bacterium rod weight, injection constituent content is 0.1 part of oleic acid, injection
Depth will be reached at the 1/2 of bacterium rod thickness.When bacterium rod mycelia is covered with substantially, second of acanthopore carried out to bacterium rod and put miscellaneous bacteria gas
During oxygenating gas, according to bacterium rod weight, injection constituent content is 0.05 part of oleic acid, and injection depth will be reached at the 1/2 of bacterium rod thickness.
This utilization bacterium rod acanthopore puts miscellaneous bacteria gas oxygenating gas process injection plant growth regulator, and process is simple, easy to operate, raw
Produce man-hour incrementss less, the polyoses content in Phellinus fructification can improve 20-25%.
Embodiment 7
With reference to embodiment 4, it is that 0.1-0.5 parts of oleic acid mix pack thoroughly that components by weight percent content is added in substrate material, in bacterium rod
The cultural hypha stage injects any one in 0.05-0.1 parts of plant growth regulator α-naphthylacetic acids or 3- indolebutyric acids, due to
The heat resistance of oleic acid is good, and heat resistanceheat resistant solution is strong, and the loss in high-temperature sterilization process is small, and utilization rate is high, in Phellinus fructification
Polyoses content, can increase 25-30%.
Claims (5)
1. a kind of cultural method for improving Phellinus polyoses content, including substrate material preparation, pack, high-temperature sterilization, inoculation, mycelia training
Foster, crop field management of producing mushroom and harvesting step, it is characterised in that in substrate material preparation, inoculation or cultural hypha step
Add plant growth regulator in any one step or any two step, the plant growth regulator be by oleic acid, α-
Any one or any two kinds in methyl α-naphthyl acetate or 3- indolebutyric acids are used cooperatively.
2. a kind of cultural method for improving Phellinus polyoses content as claimed in claim 1, it is characterised in that in the substrate material
In preparation steps, the component (weight) of the substrate material is：
Wherein, moisture content is 60~65% in the substrate material, while adding plant growth regulator in the substrate material
0.1~0.5 part, pouch step is carried out after stirring.
3. a kind of cultural method for improving Phellinus polyoses content as claimed in claim 1, it is characterised in that gone out in the high temperature
Before inoculation step starts after bacterium step terminates, it is 0.05~0.1 part biological raw that constituent content is first injected at inoculation mouth
Long conditioning agent, then carries out the inoculation step again, wherein, the depth of the plant growth regulator injection is bacterium rod thickness
2/3 position.
4. a kind of cultural method for improving Phellinus polyoses content as claimed in claim 1, it is characterised in that in mycelia training
Support in step, be when bacterium rod mycelia is covered with and bacterium rod acanthopore handle, into the bacterium rod injection constituent content for 0.05~
0.1 part of plant growth regulator, wherein, the plant growth regulator injection depth is at the 1/2 of bacterium rod thickness.
5. a kind of cultural method for improving Phellinus polyoses content as claimed in claim 1, it is characterised in that in described two steps
Suddenly, it is that the substrate material is prepared with the inoculation or cultural hypha step, is separately added into the plant growth regulator any
It is a kind of or any two kinds use cooperatively.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866289A (en) * | 2017-04-21 | 2017-06-20 | 绩溪县中巧食用菌种植专业合作社 | A kind of cultural method for improving lentinan content |
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Application publication date: 20170808 |