A kind of cultural method of hickory chick
Technical field
The present invention relates to field of edible fungus culture, more particularly to a kind of cultural method of hickory chick.
Background technology
Hickory chick, is commonly called as sheep sparrow bacterium, maize bacterium, pockmarks bacterium, wolf tripe etc., is under the jurisdiction of Ascomycotina, discomycete, cup fungi
Mesh, Morchellaceae, morchella.Hickory chick has the cellular cap of cavernous transformation, because its pole of figure like sheep tripe is referred to as sheep
Tripe bacterium.Hickory chick is described as first of " four big wild name bacterium ", imperial palace tribute, Italian top food materials, with high nutriture value
Value and medical value, are the large-scale edible and medicinal fungis of a class.It is antifatigue, anti-that modern chemistry research has shown that hickory chick has
Bacterium, it is antiviral, antitumor the effects such as, wherein the research to hickory chick active component polysaccharides is more, hickory chick is had proven at present many
Sugar has the pharmacological actions such as anti-oxidant, antibacterium, antitumor and immunological regulation.
The cultivation of hickory chick has a large amount of reports, and many of which is cultivated using wheat, and culture medium prescription is complicated, into
This is higher, and sclerotium kind is not reused in addition, such as《The indoor cultivation method for Ganoderma of hickory chick and its greenhouse used》《A kind of sheep
Tripe bacterium sclerotium rapid generation》, sclerotium kind is used in cultivation, but do not recycle and reuse, and cultivate sclerotium
Wheat is used, cost is higher.
The content of the invention
The purpose of the present invention is to solve the shortcomings of the prior art and a kind of cultural method of hickory chick is provided, the cultivation side
Sclerotium of morchella esculenta kind is repeatable using the cultivation of hickory chick is carried out in method, so as to reduce cost.
To reach above-mentioned purpose,
On the one hand, the present invention provides a kind of cultural method of hickory chick, comprises the following steps:
A, sclerotium of morchella esculenta making:Morchella mother culture is inoculated on bottled culture medium, 18-24 DEG C of light culture 30-40
My god, form sclerotium;
B, hickory chick mushroom producing culture:The sclerotium obtained in step a is seeded into cultivation matrix, management of producing mushroom 35-45 days
Ripe Morchella esculenta (L.) Pers sporophore can be harvested;
C, by after mushroom producing culture in step b sclerotium of morchella esculenta take out, repeat step b carry out hickory chick mushroom producing culture.
In certain embodiments, the present invention provides a kind of cultural method of hickory chick, wherein, by mushroom producing culture in step c
Sclerotium of morchella esculenta afterwards takes out, and further comprises:Sclerotium of morchella esculenta is carried out to be put into activation immersion bubble, then repeat step b enters
Row hickory chick mushroom producing culture.
In certain embodiments, the present invention provides a kind of cultural method of hickory chick, wherein, the activation liquid is included:
NH4NO31-3%, KH2PO40.5-1%, MgSO40.5-1%, remaining is water.
In certain embodiments, the present invention provides a kind of cultural method of hickory chick, wherein, the activation liquid is included:
NH4NO31%, KH2PO40.5%, MgSO40.5%, remaining is water.
In certain embodiments, the present invention provides a kind of cultural method of hickory chick, wherein, it is bottled described in step a
Culture medium is prepared by following steps:
With including glucose 3%, yeast extract 1%, KH2PO40.5%, MgSO40.5%, gypsum 1%, nutrient solution
Wheat bran humidity is adjusted to 50-60%, is mixed, is then loaded to culture bottle height 2/3;
By the fertile soil of the wheat bran surface covering culture bottle height 1/3 after filling, control fertile soil humidity 25%-30%;
By the bottled medium sterilization loaded.
In certain embodiments, the present invention provides a kind of cultural method of hickory chick, wherein, bottled culture medium in step a
Sterilized under the conditions of 121 DEG C.
In certain embodiments, the present invention provides a kind of cultural method of hickory chick, wherein, hickory chick described in step b goes out
In mushroom culture, the sclerotium obtained in step a is cut into 8-15cm3Fritter, be seeded into 10 pieces of density of every square metre of sowing
In cultivation matrix.
In certain embodiments, the present invention provides a kind of cultural method of hickory chick, wherein, hickory chick described in step b goes out
In mushroom culture, the temperature of management of producing mushroom is 4-22 DEG C, illumination 100-800Lux, light irradiation time 6-20h, and gas concentration lwevel is
0.3-0.4%.
In certain embodiments, cultivation matrix is made up of the raw material of following parts by weight:65 parts of peat soil, 7 parts of fertile soil,
4 parts of cow dung, 4 parts of vermiculite, 7 parts of liver moss, 7 parts of husk, 6 parts of maize straw, 4 parts of peanut shell, 4 parts of soybean peel.
In certain embodiments, the present invention provides a kind of cultural method of hickory chick, comprises the following steps:A, hickory chick bacterium
The making of core:
A, sclerotium of morchella esculenta making:
(1) preparation of culture medium:
With including glucose 3%, yeast extract 1%, KH2PO40.5%, MgSO40.5%, gypsum 1%, remaining is
The nutrient solution of water adjusts wheat bran humidity to 50-60%, mixes, and then loads to culture bottle height 2/3;
By the fertile soil of the wheat bran surface covering culture bottle height 1/3 after filling, control fertile soil humidity 25%-30%;
By the 121 DEG C of sterilizings of bottled culture medium loaded;
(2) sclerotium of morchella esculenta is formed:By 1cm31 piece of morchella mother culture be inoculated into the bottled culture obtained in step (1)
On base, 18-24 DEG C of light culture 30-40 days forms sclerotium;
B, hickory chick mushroom producing culture:
The sclerotium obtained in step a is cut into 8-15cm3Fritter, cultivation is seeded into 10 pieces of density of every square metre of sowing
Train in matrix.4-22 DEG C of temperature in mushroom room, illumination 100-800Lux, light irradiation time 6-20h are controlled, gas concentration lwevel is
0.3-0.4%, management of producing mushroom can harvest ripe Morchella esculenta (L.) Pers sporophore in 35-45 days;
C, by after mushroom producing culture in step b sclerotium of morchella esculenta take out, rinsed well, be put into comprising NH with clear water4NO3
1%, KH2PO40.5%, MgSO40.5%, remaining is reused to soak 12h in 23 DEG C of activation liquid of water as strain,
Repeat step b carries out hickory chick mushroom producing culture.
In the present invention, the component content represented by " % " is mass percent.
Beneficial effects of the present invention are as follows:
1st, the present invention provides a kind of cultural method of hickory chick, wherein uses training in sclerotium of morchella esculenta kind cultivation step
Support base:Wheat bran (puts nutrient solution and is adjusted to humidity 50-60% (nutrient solutions:Glucose 3%, yeast extract 1%, KH2PO40.5%,
MgSO40.5%, gypsum 1%, remaining is water)) under, fertile soil is upper.Without using wheat in the culture medium, only with wheat bran and
Prepared by a small amount of nutrient solution, compared with prior art, and formula is simple, and cost is low.
2nd, the sclerotium kind prepared with the present invention can be reused in the cultivation of hickory chick fruiting, greatly save cost, and
After activation immersion bubble, hickory chick yield has a certain upgrade.
3rd, hickory chick is cultivated using sclerotium of morchella esculenta kind in the present invention, prevents the pollution problem of cultigen.
Embodiment
In order that the present invention is more clearly understood, below in conjunction with instantiation, the present invention will be described in further detail.
It should be understood that these embodiments are merely to illustrate rather than limited the scope of the present invention.
Embodiment 1:The cultural method of hickory chick
The cultivation of hickory chick is completed by step a, b, c.
A, sclerotium of morchella esculenta making:
With including glucose 3%, yeast extract 1%, KH2PO40.5%, MgSO40.5%, gypsum 1%, remaining is
The nutrient solution of water adjusts wheat bran humidity to 50-60%, is fitted into after mixing in 500mL wide-mouth bottles, is filled at bottle height 2/3, surface is covered
The fertile soil of lid bottle height 1/3, fertile soil humidity 25-30%, 121 DEG C of sterilizing 2h.After cooling, every bottle of inoculation 1cm3Hickory chick it is female
Kind of 1 piece of block, 18-24 DEG C of light culture 30-40 days, that is, form sclerotium.
B, hickory chick mushroom producing culture:
Sclerotium is cut into 8-15cm3Fritter, be then seeded into hickory chick plantation substrate, substrate preparation:By with
The raw material composition of lower parts by weight:65 parts of peat soil, 7 parts of fertile soil, 4 parts of cow dung, 4 parts of vermiculite, 7 parts of liver moss, 7 parts of husk is beautiful
6 parts of stalk of rice, 4 parts of peanut shell, 4 parts of soybean peel.Every square metre is sowed 10 pieces.Control 4-22 DEG C of temperature, illumination in mushroom room
100-800Lux, light irradiation time 6-20h, gas concentration lwevel are 0.3-0.4%, and management of producing mushroom can harvest maturation in 35-45 days
Morchella esculenta (L.) Pers sporophore.
After c, fruiting terminate, sclerotium is chosen, rinsed well with clear water, is reused as strain, repeat step b, warp
Cross sowing, regulate and control Cultivation condition, management of producing mushroom 35-45 days, average every square metre of yield is of about 1.2 kilograms.
Embodiment 2:The cultural method of hickory chick
The cultivation of hickory chick is completed by step a, b, c.
A, sclerotium of morchella esculenta making:
With including glucose 3%, yeast extract 1%, KH2PO40.5%, MgSO40.5%, gypsum 1%, remaining is
The nutrient solution of water adjusts wheat bran humidity to 50-60%, is fitted into after mixing in 500mL wide-mouth bottles, is filled at bottle height 2/3, surface is covered
The fertile soil of lid bottle height 1/3, fertile soil humidity 25-30%, 121 DEG C of sterilizing 2h.After cooling, every bottle of inoculation 1cm3Hickory chick it is female
Kind of 1 piece of block, 18-24 DEG C of light culture 30-40 days, you can formation sclerotium.
B, hickory chick mushroom producing culture:
Sclerotium is cut into 8-15cm3Fritter, be then seeded into hickory chick plantation substrate, substrate preparation:By with
The raw material composition of lower parts by weight:65 parts of peat soil, 7 parts of fertile soil, 4 parts of cow dung, 4 parts of vermiculite, 7 parts of liver moss, 7 parts of husk is beautiful
6 parts of stalk of rice, 4 parts of peanut shell, 4 parts of soybean peel.Every square metre is sowed 10 pieces.Control 4-22 DEG C of temperature, illumination in mushroom room
100-800Lux, light irradiation time 6-20h, gas concentration lwevel are 0.3-0.4%, and management of producing mushroom can harvest maturation in 35-45 days
Morchella esculenta (L.) Pers sporophore.
After c, fruiting terminate, sclerotium is chosen, rinsed well with clear water, is put into 23 DEG C of activation liquid and (includes NH4NO31%,
KH2PO40.5%, MgSO40.5%, remaining is water) middle immersion 12h, reused as strain, by sowing, regulation and control fruiting
Condition, management of producing mushroom 35-45 days, average every square metre of yield is of about 1.6 kilograms.
Embodiment 3:The cultural method of hickory chick
The cultivation of hickory chick is completed by step a, b, c.
A, sclerotium of morchella esculenta making:
With including glucose 3%, yeast extract 1%, KH2PO40.5%, MgSO40.5%, gypsum 1%, remaining is
The nutrient solution of water adjusts wheat bran humidity to 50-60%, is fitted into after mixing in 500mL wide-mouth bottles, is filled at bottle height 2/3, surface is covered
The fertile soil of lid bottle height 1/3, fertile soil humidity 25-30%, 121 DEG C of sterilizing 2h.After cooling, every bottle of inoculation 1cm3Hickory chick it is female
Kind of 1 piece of block, 18-24 DEG C of light culture 30-40 days, you can formation sclerotium.
B, hickory chick mushroom producing culture:
Sclerotium is cut into 8-15cm3Fritter, be then seeded into hickory chick plantation substrate, substrate preparation:By with
The raw material composition of lower parts by weight:65 parts of peat soil, 7 parts of fertile soil, 4 parts of cow dung, 4 parts of vermiculite, 7 parts of liver moss, 7 parts of husk is beautiful
6 parts of stalk of rice, 4 parts of peanut shell, 4 parts of soybean peel.Every square metre is sowed 10 pieces.Control 4-22 DEG C of temperature, illumination in mushroom room
100-800Lux, light irradiation time 6-20h, gas concentration lwevel are 0.3-0.4%, and management of producing mushroom can harvest maturation in 35-45 days
Morchella esculenta (L.) Pers sporophore.
After c, fruiting terminate, sclerotium is chosen, rinsed well with clear water, is put into 23 DEG C of activation liquid and (includes NH4NO33%,
KH2PO41%, MgSO41%, remaining is water) middle immersion 12h, reused as strain, by sowing, regulation and control fruiting bar
Part, management of producing mushroom 35-45 days, average every square metre of yield is of about 1.5 kilograms.
Embodiment 4:The cultural method of hickory chick
Sclerotium after fruiting terminates in embodiment 2 is chosen, rinsed well with clear water, is put into 23 DEG C of activation liquid and (includes
NH4NO31%, KH2PO40.5%, MgSO40.5%, remaining is water) middle immersion 12h, used as strain third time, by broadcasting
Kind, regulation and control Cultivation condition, management of producing mushroom 35-45 days, average every square metre of yield is of about 1.5 kilograms.
Sclerotium of morchella esculenta kind is repeatable using the cultivation of hickory chick is carried out in above-mentioned cultural method, is cultivated into so as to reduce
This, available for mass producing.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.