CN111226689A - Morchella strain seed dressing agent and use method thereof - Google Patents
Morchella strain seed dressing agent and use method thereof Download PDFInfo
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- CN111226689A CN111226689A CN202010107889.9A CN202010107889A CN111226689A CN 111226689 A CN111226689 A CN 111226689A CN 202010107889 A CN202010107889 A CN 202010107889A CN 111226689 A CN111226689 A CN 111226689A
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- 241000221638 Morchella Species 0.000 title claims abstract description 78
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 68
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 30
- 229910052761 rare earth metal Inorganic materials 0.000 claims abstract description 26
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims abstract description 25
- 150000002910 rare earth metals Chemical class 0.000 claims abstract description 23
- 239000003337 fertilizer Substances 0.000 claims abstract description 22
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 15
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 15
- 229910021538 borax Inorganic materials 0.000 claims abstract description 14
- 239000004328 sodium tetraborate Substances 0.000 claims abstract description 14
- 235000010339 sodium tetraborate Nutrition 0.000 claims abstract description 14
- 239000011721 thiamine Substances 0.000 claims abstract description 14
- 229960003495 thiamine Drugs 0.000 claims abstract description 14
- 235000019157 thiamine Nutrition 0.000 claims abstract description 14
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 14
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 14
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 14
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000004202 carbamide Substances 0.000 claims abstract description 13
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 13
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 12
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 12
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 11
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims abstract 3
- 240000002769 Morchella esculenta Species 0.000 claims description 21
- 235000002779 Morchella esculenta Nutrition 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 3
- 230000012010 growth Effects 0.000 abstract description 22
- 235000016709 nutrition Nutrition 0.000 abstract description 17
- 230000035764 nutrition Effects 0.000 abstract description 16
- 239000002689 soil Substances 0.000 abstract description 13
- 230000035784 germination Effects 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 11
- 241000894007 species Species 0.000 abstract description 6
- 241000233866 Fungi Species 0.000 abstract description 2
- 238000011282 treatment Methods 0.000 description 15
- 238000009331 sowing Methods 0.000 description 9
- 239000011573 trace mineral Substances 0.000 description 7
- 235000013619 trace mineral Nutrition 0.000 description 7
- 238000007796 conventional method Methods 0.000 description 6
- 239000008187 granular material Substances 0.000 description 5
- 238000004898 kneading Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000723418 Carya Species 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000010899 nucleation Methods 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229910052796 boron Inorganic materials 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- ZSLUVFAKFWKJRC-IGMARMGPSA-N 232Th Chemical compound [232Th] ZSLUVFAKFWKJRC-IGMARMGPSA-N 0.000 description 1
- 229910052684 Cerium Inorganic materials 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 229910052779 Neodymium Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 229910052777 Praseodymium Inorganic materials 0.000 description 1
- 229910052776 Thorium Inorganic materials 0.000 description 1
- 229910052770 Uranium Inorganic materials 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- GWXLDORMOJMVQZ-UHFFFAOYSA-N cerium Chemical compound [Ce] GWXLDORMOJMVQZ-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229910052746 lanthanum Inorganic materials 0.000 description 1
- FZLIPJUXYLNCLC-UHFFFAOYSA-N lanthanum atom Chemical compound [La] FZLIPJUXYLNCLC-UHFFFAOYSA-N 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- QEFYFXOXNSNQGX-UHFFFAOYSA-N neodymium atom Chemical compound [Nd] QEFYFXOXNSNQGX-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- PUDIUYLPXJFUGB-UHFFFAOYSA-N praseodymium atom Chemical compound [Pr] PUDIUYLPXJFUGB-UHFFFAOYSA-N 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000031877 prophase Effects 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- DNYWZCXLKNTFFI-UHFFFAOYSA-N uranium Chemical compound [U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U] DNYWZCXLKNTFFI-UHFFFAOYSA-N 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 239000009137 wuling Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a morchella strain seed dressing agent and a use method thereof, and relates to the technical field of edible fungus cultivation. The morchella strain seed dressing agent comprises, by weight, 20-30 parts of urea, 10-20 parts of monopotassium phosphate, 10-20 parts of magnesium sulfate, 0.5-3 parts of borax, 0.5-3 parts of zinc sulfate, 0.5-3 parts of rare earth micro-fertilizer and 0.5-3 parts of thiamine. When in use, the morchella strain seed dressing agent is mixed with morchella cultivated species according to the weight ratio of 0.002-0.02% and is sowed. The morchella strain seed dressing agent can quickly recover the growth activity of morchella strains, promote hypha germination, enhance the capacity of the hypha to absorb nutrition from soil and a nutrition bag, accelerate the healthy growth of the hypha and further achieve the purpose of increasing the yield.
Description
Technical Field
The invention relates to the technical field of edible fungus cultivation, in particular to a morchella strain seed dressing agent and a using method thereof.
Background
Sichuan basins, Sichuan plateaus, Qinba mountains, Wuling mountains, Wumeng mountains and Yunobu plateaus are main cultivation areas of morchella esculenta, and farmland cultivation without base materials is a main cultivation mode. When the morchella is cultivated in a farmland without base materials, strains are sown in soil, and as the strains are taken out from a container and kneaded into granules to damage hyphae, the growth environment is changed, the revival capacity of the strains is reduced, the hyphae germinate slowly, the capacity of the morchella hyphae for absorbing nutrition from the soil (a nutrition bag is placed about 10 days after sowing) is weak, and nutrition is urgently needed to promote the hyphae to quickly recover the growth vigor.
Patent CN108546181A discloses a morchella seeding and seed dressing yield increasing agent, which comprises the following components: 20g of dipotassium phosphate, 10g of magnesium sulfate, 30g of yeast extract and 940g of oil tea cake. The main problem that this patent was solved provides the required nutrition of hickory chick hypha in the vegetative phase after the hickory chick bacterial is sowed, and its effect has increased hickory chick hypha and has supplied nutrition in the process of growing in addition to soil and nutrition bag and obtain the channel, does not solve the reduction of strain reviving power after the bacterial is sowed, the problem that the hypha germinates slowly.
Disclosure of Invention
In view of the above, the invention provides a morchella strain seed dressing agent, which can improve the revival capability of morchella strains after being sown, promote the hypha to germinate rapidly, enhance the capacity of the hypha to absorb nutrition from soil and a nutrition bag, and accelerate the growth speed of the hypha, thereby achieving the purpose of increasing yield.
The technical scheme of the invention is as follows:
the invention provides a morchella strain seed dressing agent which comprises, by weight, 20-30 parts of urea, 10-20 parts of monopotassium phosphate, 10-20 parts of magnesium sulfate, 0.5-3 parts of borax, 0.5-3 parts of zinc sulfate, 0.5-3 parts of rare earth micro-fertilizer and 0.5-3 parts of thiamine.
Preferably, the morchella strain seed dressing agent comprises 20 parts of urea, 15 parts of monopotassium phosphate, 11 parts of magnesium sulfate, 1 part of borax, 1 part of zinc sulfate, 1 part of rare earth micro-fertilizer and 1 part of thiamine.
Further preferably, the morchella strain seed dressing agent is prepared by uniformly mixing the components.
The invention also comprises a using method of the morchella strain seed dressing agent, and the morchella strain seed dressing agent is mixed into morchella cultivars according to the weight ratio of 0.002-0.02%.
Preferably, the morchella cultivar is granular.
Preferably, the morchella esculenta strain seed dressing agent is dissolved and then mixed with morchella esculenta cultivated species.
Furthermore, the morchella strain seed dressing agent is prepared from (1-10) g: (1-5) kg of the composition was dissolved in water.
Further, the morchella strain seed dressing agent is prepared by mixing the following components in percentage by weight according to 1: 500 parts by weight were dissolved in water.
Preferably, the morchella strain seed dressing agent is mixed into morchella cultivars and sowed.
Compared with the prior art, the invention has the following advantages:
compared with the prior art, the morchella strain seed dressing agent developed by the invention is scientifically prepared by combining the nutrient condition of Guizhou soil according to the nutritional characteristics of morchella, and is matched with a using method of the morchella strain seed dressing agent, so that the growth activity of the morchella strain can be quickly recovered, hypha germination is promoted, the capacity of the hypha for absorbing nutrition from soil and a nutrition bag is enhanced, the healthy growth of the hypha is accelerated, and the purpose of increasing yield is achieved. The application of the invention is simple, convenient, low in cost and obvious in effect, and provides a novel seed dressing agent with strong pertinence and specificity for morchella production.
Drawings
FIG. 1 is a graph showing the effect of the treatment of the dressing agent in example 4 on the growth of morchella esculenta.
FIG. 2 is a graph showing the effect of the treatment of the dressing agent in example 4 on the growth of morchella esculenta.
FIG. 3 is a graph showing the growth effect of morchella esculenta after the blank treatment of example 4 and 3.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a morchella strain seed dressing agent for improving the revivification of the strain after morchella seeding, which comprises 20-30 parts of urea, 10-20 parts of monopotassium phosphate, 10-20 parts of magnesium sulfate, 0.5-3 parts of borax, 0.5-3 parts of zinc sulfate, 0.5-3 parts of rare earth micro-fertilizer and 0.5-3 parts of thiamine by weight.
According to the morchella strain seed dressing agent, urea is adopted to provide a nitrogen source, and compared with organic nitrogen sources such as yeast extract, oil-tea cake and the like, the morchella strain seed dressing agent is more rapid in effect, can rapidly promote activation of morchella strain cultivated species, and provides a foundation for rapid growth and germination of hypha of morchella strain cultivated species. In the invention, the urea accounts for 20-30 parts by weight, preferably 23-27 parts by weight based on the total weight of the morchella strain seed dressing agent. The potassium dihydrogen phosphate and the magnesium sulfate are used as nutrient elements for the growth of the morchella, and also play a promoting role in the activation and growth processes of strains. In the invention, the total weight of the morchella strain seed dressing agent is 10-20 parts by weight, preferably 13-17 parts by weight; the magnesium sulfate is 10 to 20 parts by weight, preferably 11 to 17 parts by weight.
The morchella strain seed dressing agent is also added with borax, zinc sulfate and rare earth trace element fertilizer. Boron, zinc and rare earth elements can activate the bioactivity of the morchella metal activating enzyme, improve the revival force of morchella strains and facilitate the quick germination of strain hypha. Meanwhile, the borax, the zinc sulfate and the rare earth micro-fertilizer are also suitable for the characteristics that most Guizhou soils are yellow soils, and the boron, the zinc and the rare earth elements are deficient, and are very suitable for the application of morchella planting fields. In the invention, the borax accounts for 0.5-3 parts by weight, preferably 1-2 parts by weight, based on the total weight of the morchella strain seed dressing agent; 0.5-3 parts of zinc sulfate, preferably 1-2 parts; the rare earth micro-fertilizer is 0.5-3 weight parts, preferably 1-2 weight parts.
In the invention, the rare earth trace element fertilizer refers to water-soluble salts (containing no radioactive substances such as uranium, thorium and the like) of a light rare earth element compound. The rare earth trace element fertilizer used in the invention adopts the rare earth trace element fertilizer known in the prior art. At present, the agricultural rare earth micro-fertilizer mainly comprises two elements of lanthanum (La) and cerium (Ce), including a small amount of praseodymium (Pr) and neodymium (Nd), and an inorganic salt or organic salt of light rare earth, usually nitrate [ R (NO)3)2]A mixture of (a). The invention has no limit to the source of the rare earth trace element fertilizer.
The toadstool strain seed dressing agent of the invention is also added with thiamine, namely vitamin B1. The tiaanin is an essential factor for growth of the morchella esculenta, but the morchella esculenta cannot synthesize the tiaanin. The toadstool strain seed dressing agent ensures the necessary growth factors required by the toadstool in the initial stage of growth and germination by adding the thiamine, accelerates the rapid growth of toadstool hyphae, and solves the problem of slow germination of the toadstool hyphae. In the invention, the thiamine accounts for 0.5-3 parts by weight, preferably 1-2 parts by weight of the total weight of the morchella strain seed dressing agent.
The seed dressing agent provided by the invention can obviously improve the reviving capability of the morchella strain, promote the hypha to germinate rapidly, enhance the capacity of the hypha to absorb nutrition from soil and a nutrition bag, accelerate the growth speed of the hypha and further achieve the purpose of increasing the yield.
As an embodiment, the morchella strain seed dressing agent comprises 20 parts of urea, 15 parts of monopotassium phosphate, 11 parts of magnesium sulfate, 1 part of borax, 1 part of zinc sulfate, 1 part of rare earth trace fertilizer and 1 part of thiamine.
As an embodiment, the components of the seed dressing agent are uniformly mixed to obtain the morchella strain seed dressing agent.
When the morchella seed dressing agent is used, the morchella seed dressing agent is mixed with morchella cultivars for use, and the weight ratio of seed dressing is 0.002% -0.02%, preferably 0.005% -0.015%, and more preferably 0.01-0.012%.
As an embodiment, the morchella cultivar of the present invention is granular. The conventional morchella is prepared by taking morchella cultivars out of a strain bottle or a strain bag before mixing with a seed dressing agent, kneading into granules, and conveniently mixing and sowing.
As one embodiment, the morchella species dressing agent of the present invention is melted before use. In one embodiment, the morchella species dressing agent is dissolved in water. Preferably 1-10g of the seed-dressing agent is fully dissolved by adding 1-5kg of water, more preferably 5g of the seed-dressing agent is fully dissolved by adding 2.5kg of water, and the dosage proportion of the water can be adjusted by a person skilled in the art according to the dissolving degree.
As an embodiment, the morchella strain seed dressing agent of the present invention is mixed into morchella cultivars, and the morchella cultivars mixed with the seed dressing agent are sown according to a conventional method. The method can be directly sown in planting soil or cultivation material, the cultivation mode of the morchella is not limited, and the method can be carried out according to the conventional operation of a person skilled in the art.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Example 1
20 parts of urea, 15 parts of monopotassium phosphate, 11 parts of magnesium sulfate, 1 part of borax, 1 part of zinc sulfate, 1 part of rare earth micro-fertilizer and 1 part of thiamine are uniformly mixed to prepare the morchella strain seed dressing agent.
The using method comprises the following steps: taking out the morchella esculenta cultivars from a strain bottle or a strain bag, kneading the morchella esculenta cultivars into granules, adding 2.5kg of water into 5g of prepared seed-dressing agent, fully dissolving, uniformly mixing 50kg of morchella esculenta cultivars, and then sowing according to a conventional method.
Example 2
25 parts of urea, 12 parts of monopotassium phosphate, 15 parts of magnesium sulfate, 1.5 parts of borax, 1.5 parts of zinc sulfate, 2 parts of rare earth micro-fertilizer and 2 parts of thiamine are uniformly mixed to prepare the morchella strain seed dressing agent.
The using method comprises the following steps: taking out the morchella esculenta cultivars from a strain bottle or a strain bag, kneading the morchella esculenta cultivars into granules, adding 3.5kg of water into 7g of prepared seed-dressing agent, fully dissolving, uniformly mixing 50kg of morchella esculenta cultivars, and then sowing according to a conventional method.
Example 3
30 parts of urea, 18 parts of monopotassium phosphate, 17 parts of magnesium sulfate, 2 parts of borax, 2 parts of zinc sulfate, 1 part of rare earth micro-fertilizer and 2.5 parts of thiamine are uniformly mixed to prepare the morchella strain seed dressing agent.
The using method comprises the following steps: taking out the morchella esculenta cultivars from a strain bottle or a strain bag, kneading the morchella esculenta cultivars into granules, adding 2kg of water into 3g of prepared seed-dressing agent, fully dissolving, uniformly mixing 50kg of morchella esculenta cultivars, and then sowing according to a conventional method.
Example 4
Application examples
1. Materials and methods
1.1 test materials
Strain: the variety is six sisters, and is separated by spores and preserved. The mother culture medium is a PDA culture medium; the stock and cultivated species are wheat strains.
Rare earth trace element fertilizer: the rare earth trace element foliar fertilizer is purchased in the market, including Hua nong rare earth fertilizer industry Co.
1.2 test methods
1.2.1 test design
Design 3 treatments, treatment 1: 20g of dipotassium phosphate, 10g of magnesium sulfate, 30g of yeast extract and 940g of oil tea cake; and (3) treatment 2: 20g of urea, 15g of monopotassium phosphate, 11g of magnesium sulfate, 1g of borax, 1g of zinc sulfate, 1g of rare earth micro-fertilizer and 1g of thiamine; process 3 (CK): no seed dressing agent is used. 3 replicates per treatment, 10m per replicate2。
1.2.3 isolation, preservation and production of Strain
The strain is preserved by a conventional method after spore separation, and the stock and the cultivated species are produced by a conventional production method of the wheat strain.
1.2.3 sowing
Seeding 12 months and 12 days in 2019, taking out the strains, kneading, and respectively mixing with the seed dressing agent prepared according to the treatment 1 and the treatment 2; 1, mixing 10kg of seed dressing agent into every 100kg of strains; treatment 2, adding 2.5kg of water into 5g of prepared seed dressing agent, fully dissolving, and uniformly mixing 50kg of morchella esculenta cultivated seeds; process 3 (CK): no seed dressing agent is used. Then directly sowing seeds on the surface of the compartment according to the seed consumption of 0.8kg per square meter, immediately covering with fine soil with the thickness of 3cm, and managing the illumination, the temperature and the moisture according to a conventional method.
1.2.4 recording and Observation
And observing and recording the germination time, hypha growth vigor and conidium appearance time and quantity of the strains treated each day after sowing.
2. Test results
After 3 days of sowing, 3 treatments can see germination and colonization of morchella hyphae by naked eyes, but the germination starting time of each treatment is different, and the details are shown in table 1 and figures 1-3.
TABLE 1 use conditions of different morchella dressing agents
| Different treatment | Time for hypha to start germination | Growth of hyphae | Time of conidiophore appearance | Number of conidia |
| Process 1 | 4 days | White and denser | 9 | Much more |
| Treatment 2 | 2 days | White, dense | 6 | Multiple purpose |
| Process 3(CK) | 4 days | Sparse | 10 | Is less |
3. Summary and discussion
Although the test only observes the prophase of the vegetative growth of the morchella, the test has obvious difference. The best effect of the treatment 2 is seen from the comprehensive aspects of hypha germination, hypha growth, conidium appearance and quantity. The seed dressing agent and the use method thereof are closely related to the fact that the growth activity of morchella strains can be rapidly recovered, hypha germination is promoted, the capacity of the hypha for absorbing nutrition from soil and a nutrition bag is enhanced, and healthy growth of the hypha is accelerated. The seed dressing agent for treating 2 has simple and convenient application, low cost and obvious effect.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (9)
1. A morchella strain seed dressing agent comprises, by weight, 20-30 parts of urea, 10-20 parts of monopotassium phosphate, 10-20 parts of magnesium sulfate, 0.5-3 parts of borax, 0.5-3 parts of zinc sulfate, 0.5-3 parts of rare earth micro-fertilizer and 0.5-3 parts of thiamine.
2. The morchella strain seed dressing agent according to claim 1, which comprises 20 parts of urea, 15 parts of monopotassium phosphate, 11 parts of magnesium sulfate, 1 part of borax, 1 part of zinc sulfate, 1 part of rare earth microelement fertilizer and 1 part of thiamine.
3. The morchella species seed dressing agent according to claim 1 or 2, characterized in that it is obtained by uniformly mixing the components.
4. The use method of the morchella strain seed dressing agent according to any one of claims 1 to 3, characterized in that the morchella strain seed dressing agent is mixed into morchella cultivars according to the weight ratio of 0.002-0.02%.
5. The use of claim 4, wherein the Morchella esculenta cultivar is particulate.
6. The use method of claim 4, wherein the Morchella esculenta strain seed dressing agent is dissolved and then mixed with Morchella esculenta strain culture.
7. The use method of claim 6, wherein the morchella strain seed dressing agent is prepared from (1-10) g: (1-5) kg of the composition was dissolved in water.
8. The use method of claim 7, wherein the morchella species dressing agent is applied to the morchella species in a ratio of 1: 500 parts by weight were dissolved in water.
9. The use method according to any one of claims 4 to 7, wherein the morchella strain seed dressing agent is mixed with morchella cultivars and sown.
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| CN114521451A (en) * | 2022-03-24 | 2022-05-24 | 绵阳市经科菌业有限责任公司 | Method for cultivating morchella strain with high survival rate |
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