KR101629793B1 - A medium for production of morchella esculenta - Google Patents

Abstract

The present invention relates to a medium composition for the production of Morchella esculenta , and more particularly to a medium composition comprising a Morel mushroom seedling culture medium, a Morel mushroom culture organism culture medium and a Morelia mushroom culture medium.
The medium composition for the production of morel mushroom according to the present invention has the effect of solving the problem of depletion of resources and destruction of ecosystem in the wild collection of Morel mushroom fruiting body.
The medium composition for the production of morel mushroom according to the present invention has the effect of shortening the cultivation period of Morel mushroom and the effect of reducing the cultivation cost, and it has an effect of contributing to the profit increase by increasing the production amount of Morel mushroom.

Description

A MEDIUM FOR PRODUCTION OF MORCHELLA ESCULENTA BACKGROUND OF THE INVENTION [0001]

The present invention relates to a medium composition for the production of Morchella esculenta , and more particularly to a medium composition comprising a Morel mushroom seedling culture medium, a Morel mushroom culture organism culture medium and a Morelia mushroom culture medium.

In the production of the morel mushroom seedling culture medium, 900 to 1100 parts by weight of water, 400 to 600 parts by weight of potato extract, 10 to 30 parts by weight of dextrose, 10 to 30 parts by weight of agar and 0.5 to 1.5 parts by weight of peptone are contained And is manufactured in accordance with a technical feature.

The present invention is characterized in that, in the production of the culture medium of Morel mushroom cultivar, 50 to 70 parts by weight of oak fermented sawdust, 20 to 30 parts by weight of fermented tulipwood, and 10 to 20 parts by weight of rice bran are prepared.

The present invention relates to a method for producing a culture medium of Morel mushroom mycelium comprising the steps of: mixing 15-25 parts by weight of sugar, 2-5 parts by weight of soybean meal, 2-3 parts by weight of MgSO ₄ , 1-2 parts by weight of KH ₂ PO ₄ , And 0.5 to 1.5 parts by weight of an anti-foam.

The medium composition for the production of morel mushroom according to the present invention has the effect of solving the problem of depletion of resources and destruction of ecosystem due to the collection of conventional Morel mushroom fruiting body by enhancing the production amount of morel mushroom.
The medium composition for the production of morel mushroom according to the present invention has an effect of contributing to the profit increase of morel mushroom by shortening the cultivation period of morel mushroom and reducing the cultivation cost.

Morchella esculenta is a mushroom belonging to the mushroom, one of the world's most valuable rare edible bacteria, and contains protein, carbohydrates, vitamins and amino acids.

Recently, the medicinal component of Morel mushroom has been attracting attention. It has been reported that it contains a high concentration of organic germanium to improve kidney function and ovary, improve concentration, and have therapeutic effect on gastroenteritis, dyspepsia and cancer.

However, in spite of the above-mentioned advantages of Morel mushroom, Morel mushroom was not widely known in Korea.

One of the conventional methods of obtaining morel mushrooms is to collect wild fruiting bodies of morel mushrooms. However, the method of collecting wild fruiting bodies is disadvantageous in that resources are depleted, ecosystems are destroyed, and mass quantities can not be secured.

Another conventional method for obtaining myrrh mushroom is to isolate mycelium from myrrh mushroom fruiting body, cultivate the mycelium, and grow.

The cultivation of Morel mushroom was started in England, USA and France in the 1880s. Since the 1960s, cultivation methods of Morel mushroom mycelium have been developed in USA and China, and the mycelium produced by the above method has been used to produce foods and medicines.

However, the mycelial cultivation method takes a long period of cultivation and has a disadvantage in that it is impossible to secure a large amount of water in the same manner as the method of collecting wild fruit bodies.

There has been no successful case of mass cultivation of Morel mushroom. Research on the cultivation of Morel mushroom in Korea is only an early stage. Therefore, there is absolutely no description about how to produce Morel mushroom at home and abroad.

Accordingly, the Applicant has determined that it is possible to provide a medium composition for massive cultivation of Morel mushroom, thereby disclosing the present invention.

In the prior art related to the present invention, Korean Patent Laid-Open Publication No. 10-1992-0702604 discloses a method of mixing and cultivating mushroom mycelium in a cereal mixture which has been boiled and then cooled, 1206324 describes a method for producing a mycelium of mushroom mycorrhizae by culturing the mushroom in a solid medium containing pumice.

Korean Patent Registration No. 10-0071463 also discloses a method for cultivating a morcella (Morel mushroom) comprising culturing a mycelium, inoculating the medium, supplying a nutrient source, and removing the nutrient source.

Although the foregoing prior art documents describe the cultivation method of Morel mushroom, the present invention does not have the effect of increasing the production amount of Morel mushroom by stepwise culturing the seedling bacterium and the original bacterium in different formulations.

Korean Patent Laid-Open Publication No. 10-1992-0702604 (October 10, 1992) Korean Registered Patent No. 10-1206324 (November 23, 2012) Korean Registered Patent No. 10-0071463 (March 03, 1994)

It is an object of the present invention to provide a culture composition of morel mushroom which improves the yield and solves the problem of depletion of resources and destruction of ecosystems in collecting conventional morel mushroom fruiting bodies.

It is an object of the present invention to provide a medium composition having the effect of shortening the cultivation period of morel mushroom and reducing the cultivation cost, thereby enhancing the yield of morel mushroom to increase profit.

Disclosure of the Invention The present invention aims at solving the technical problem by providing a medium composition comprising a morel mushroom seedling culture medium, a morel mushroom culture medium and a morel mushroom culture medium.

In the production of the morel mushroom seedling culture medium, 900 to 1100 parts by weight of water, 400 to 600 parts by weight of potato extract, 10 to 30 parts by weight of dextrose, 10 to 30 parts by weight of agar and 0.5 to 1.5 parts by weight of peptone are contained The present invention also provides a culture medium containing the above-mentioned culture medium.

The present invention provides a culture medium comprising the above-mentioned culture medium comprising 50 to 70 parts by weight of oak fermented sawdust, 20 to 30 parts by weight of tulipwood fermented sawdust, and 10 to 20 parts by weight of rice bran at the time of preparing the culture medium of Morel mushroom fungus. Thereby solving the technical problem.

The present invention relates to a method for producing a culture medium of Morel mushroom mycelium comprising the steps of: mixing 15-25 parts by weight of sugar, 2-5 parts by weight of soybean meal, 2-3 parts by weight of MgSO ₄ , 1-2 parts by weight of KH ₂ PO ₄ , And 0.5 to 1.5 parts by weight of an antifoam. The present invention is directed to solve the technical problems.

 The present invention has the effect of solving the problem of depletion of resources and destruction of ecosystem by collecting meringue fruiting body of the present invention by promoting the production amount of morel mushroom.

The present invention has the effect of shortening the cultivation period of Morel mushroom and increasing the profit of Morel mushroom by reducing the cultivation cost.

Fig. 1 is a photograph showing the growth state of the inoculated mashinole mushroom seedlings in the No. 5 medium.
Fig. 2 is a photograph showing the growth state of morel mushroom seedlings on the 15th day of culture in the No. 5 medium.
Fig. 3 is a photograph showing the growth state of the inoculated mashinol mushroom seedlings in the No. 3 medium.
Fig. 4 is a photograph showing the growth state of Morel mushroom seedlings on the 15th day of culture in medium No. 3.
Fig. 5 is a photograph of the morula mushroom seedlings grown at day 4 on the No. 5 medium at a magnification of 10x2. Fig.
Fig. 6 is a photograph showing the morula mushroom seedlings on the 14th day of cultivation in the No. 5 medium at a magnification of 10x2. Fig.
Fig. 7 is a photograph of a morula mushroom bacterium of the 14th day cultured in a No. 5 medium at a magnification of 10 × 5.
Fig. 8 is a photograph of the morula mushroom seedlings cultured on day 14 in culture medium 5 at a magnification of 10x5. Fig.
FIG. 9 is a photograph showing growth rates of oak fermented sawdust and other additives in different weight ratios. (On the left, No. 1 medium - oak fermentation sawdust 85: rice bran 15/2 medium - oak fermentation sawdust 32.5: oak sawdust 52.5: rice bran 15/3 medium - oak fermentation sawdust 32.5: poplar wood sawdust 52.5: rice bran 15)
10 to 11 are photographs of spores of Morel mushroom mycelium observed under a microscope.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, it should be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined in the appended claims. It should be understood that various equivalents and modifications may be present.

Example  One. Meringue  culture

(One) Moxa  Step of producing medium

Mix nutrient source to make seedling culture medium.

The nutrient source may be selected from one or more kinds of potato extract, whole wheat extract, bean sprouts extract, dextrose, peptone, glucose and yeast extract, and the like, but is not limited thereto.

The weight ratio at which each nutrient source is added can be set in various ways. For example, 900 to 1100 parts by weight of water, 400 to 600 parts by weight of potato extract, 10 to 30 parts by weight of dextrose, 10 to 30 parts by weight of agar, and 0.5 to 1.5 parts by weight of peptone.

Conditions such as temperature and pH of the medium can also be set variously. But preferably the temperature is set to 20 to 30 ° C and the pH is 5 to 10, more preferably the temperature is set to 22 to 27 ° C and the pH is set to 6 to 7, but the present invention is not limited thereto.

Further, additives such as antifoam and agar may be further added, and the additives are not limited thereto.

(2) Moxa  On the badge Morel mushroom The  Culturing step

Morel mushroom seedlings are cultured in a seedling culture medium.

(3) Step of producing solid medium

1) Selecting medium of solid medium

The solid medium may be selected from sawdust, rice straw, rice husks, rice bran, etc., and may be added in various ways. Preferably, sawdust may be used, but the present invention is not limited thereto.

The sawdust material may be, but not limited to, oak, tulip, mulberry, fermented oak, fermented tulip, and pine.

The weight ratio of the solid medium may be variously set. For example, 50 to 70 parts by weight of oak fermentation sawdust, 20 to 30 parts by weight of tulip tree fermented sawdust, and 10 to 20 parts by weight of rice bran can be used.

2) adding the nutrient source

Add nutrients to the solid medium.

The nutrient source may be selected from bran, rice bran, soybean meal, sugar, CaCO ₃ (calcium carbonate), KH ₂ PO ₄ (potassium dihydrogen phosphate) and NH ₄ Cl (ammonium chloride) But is not limited thereto.

The weight ratio at which each nutrient source is added can be set in various ways.

Conditions such as temperature and pH of the medium can also be set variously.

Further, additives such as anti-foam and agar may be further added, and the additives are not limited thereto.

(4) To a solid medium Morel mushroom The  Culturing step

Morel mushroom seedlings are cultured in a solid medium to obtain morel mushroom fungus.

(5) Step of producing culture medium

A nutrient source is mixed to prepare a culture solution for culturing Morel mushroom mycelium.

Nutrient sources include sugar, CaCO ₃ , MgSO ₄ , KH ₂ PO ₄ , peptone, and soybean meal, but the present invention is not limited thereto.

For example, 15-25 parts by weight of sugar, 2-5 parts by weight of soybean meal, 2-3 parts by weight of MgSO ₄ , 1-2 parts by weight of KH ₂ PO ₄ , 0.5 part by weight of peptone 0.5 To 1.5 parts by weight and anti-foam 0.5 to 1.5 parts by weight.

Conditions such as temperature and pH of the medium can also be set variously.

Further, an additive such as an anti-foam may be further added, and the additive is not limited thereto.

(6) Morel mushroom Won Jong Kyun  Culturing step

Morel mushroom mycelium is cultured in the culture to obtain morel mushroom mycelium.

Experimental Example  1. Depending on medium composition Morel mushroom Monocotyledonous  Growth rate measurement

(1) Experimental process

In order to examine the medium composition having the highest growth potential of Morel mushroom, the morel mushroom seedlings were cultured in the group 7, which was prepared by preparing the seedling medium described in Example 1 but having different components of the medium, Diameter and density were measured to confirm the growth rate. At this time, the temperature of the incubator was set to 25 ± 1 ° C.

Cultivation of morel mushroom cultivar was carried out for 12-14 days.

(2) Experimental results

Table 1 below shows the results of comparing growth of morel mushroom seedlings with different composition of medium.

turn Medium composition component Moxa
density Moxa
Diameter (cm) One 500 g of bean sprouts extract, 20 g of sugar, 20 g of agar, and 1 L of water ++ 4.3 2 500 g of oak sawdust extract, 20 g of sugar, 20 g of agar and 1 L of water ++ 4.7 3 200 g of potato extract, 20 g of dextrose, 20 g of agar, and 1 L of water (PDA) +++ 4.9 4 500 g of whole-wheat extract, 20 g of dextrose, 20 g of agar and 1 L of water +++ 5.2 5 500 g of potato extract, 20 g of dextrose, 20 g of agar, 1.0 g of peptone and 1 L of water (PDAP) +++ 5.6 6 1.0 g of peptone, 20 g of glucose, 20 g of agar, 1.0 g of KH ₂ PO ₄ ,
0.5 g CaCO ₃ and 1 L water +++ 4.7 7 1.0 g of peptone, 20 g of glucose, 20 g of agar, 1.0 g of yeast concentrate, 1.0 g of KH ₂ PO ₄ , 0.5 g of CaCO ₃ and 1 L of water +++ 4.8

Based on the results shown in Table 1, the morel mushroom seedlings were cultured in medium 3 to 5 containing dextrose in the composition as compared with the medium 1 or 2, and in the medium 6 to 7 containing the peptone and glucose in the composition, And the diameters of the seedlings were higher than those of the seeds of the seedlings of 6 to 7 times.

Among the 3 ~ 5 mediums, the density of Morelia mushroom was highest and the diameter of the seedlings was the largest in the 5th medium (PDAP). In other words, it was confirmed that the growth efficiency was the best in the No. 5 medium.

Fig. 1 is a photograph showing the growth state of the inoculated mashinole mushroom seedlings in the No. 5 medium.

Fig. 2 is a photograph showing the growth state of morel mushroom seedlings on the 15th day of culture in the No. 5 medium.

Fig. 3 is a photograph showing the growth state of the inoculated mashinol mushroom seedlings in the No. 3 medium.

Fig. 4 is a photograph showing the growth state of Morel mushroom seedlings on the 15th day of culture in medium No. 3.

As a result of the observation, it was found that the culture was more efficiently performed when the morel mushroom seedlings were cultured in the No. 5 medium than the No. 3 medium.

Fig. 5 is a photograph of the morula mushroom seedlings grown at day 4 on the No. 5 medium at a magnification of 10x2. Fig.

Fig. 6 is a photograph of the morula mushroom seedlings cultured on day 14 in culture medium 5 at a magnification of 10x5. Fig.

Fig. 7 is a photograph of a morula mushroom bacterium of the 14th day cultured in a No. 5 medium at a magnification of 10 × 5.

Fig. 8 is a photograph of the morula mushroom seedlings cultured on day 14 in culture medium 5 at a magnification of 10x5. Fig.

Microscopic observation revealed that about 80% of the cultivation was performed when the morel mushroom seedlings were cultured in the No. 5 medium.

As a result, it can be concluded that the growth efficiency of the morel mushroom seedlings cultured by the culture method of the present invention is excellent.

Experimental Example  2. Temperature dependent Morel mushroom Monocotyledonous  Growth rate measurement

(1) Experimental process

In order to measure the growth of morel mushroom, the growth temperature was varied at 20, 28, 35 and 40 ℃, and the diameter and density of the seedling were measured. Respectively.

In Experimental Example 1, morel mushroom seedlings were cultured in a No. 5 medium (weight ratio: 500 g of potato extract, 20 g of dextrose, 20 g of agar, 1.0 g of peptone, and 1 L of water) Cultivation of mushroom seedlings was carried out for 12 to 14 days.

(2) Experimental results

[Table 2] shows the growth of Morel mushroom seedlings by temperature.

Temperature (℃) 20 24 28 35 40 Diameter (cm) 2.5 4.9 5.8 1.4 0 Seedling density ++ +++ +++ ++ X

Based on the results of Table 2, it can be seen that the growth rate of Morel mushroom seedlings is high at a temperature of 20 to 35 ° C, the highest at 24 to 28 ° C, and the growth is stopped at a temperature of more than 40 ° C.

Experimental Example  3. Morel mushroom Moxa On growth rate  About pH Measure the impact of

(1) Experimental process

For measuring the growth of Morelia mushroom, the pH and pH were varied to 4.0, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 and 9.0, respectively. The growth rate of seedlings was measured at each temperature.

In the same manner as in Experimental Example 2, the experiment was conducted by cultivating Morelia mushroom seedlings in the PDAP medium having the best growth characteristics in Experimental Example 1, and the Morelia mushroom seedlings were cultured for 12 to 14 days.

pH control was performed using a standard buffered biological buffer solution.

(2) Experimental results

[Table 3] shows the effect of pH on the growth of Morelia mushroom.

pH 4.0 5.0 5.5 6.0 6.5 7.0 7.5 8.0 9.0 density + ++ ++ +++ +++ +++ ++ ++ +

As shown in Table 3, the growth of Morelia mushroom was + at pH 4.0, ++ at 5.0, ++ at 5.5, +++ at 6,0, +++ at 6.5, +++ at 7.0 , ++ at 7.5, ++ at 8.0, and + at 9.0, and the growth of morel mushroom seedlings was relatively good at pH 5.0 ~ 8.0.

Experimental Example  4. Depending on the solid medium Morel mushroom Won Jong Kyun's  Growth rate comparison

(1) Experimental process

In order to examine the culture method that can be most effectively grown when cultivating Morelia mushroom bacillus subtilis, it was produced by the process of producing the native bacilli described in Example 1, except that oak sawdust, oak fermented sawdust, tulipwood sawdust and tulip tree fermented sawdust Five different solid mediums were prepared, and the diameter and density of the morel mushroom cultivated in each solid medium were measured to determine the growth rate of the different species according to each solid medium.

To each medium, rice bran, bran, CaCO ₃ And KH ₂ PO ₄ were mixed and injected into a plastic bottle and cultured for 12 to 14 days in a deep pear culture setting condition of pH 6.3 ± 0.2 and temperature 25 ± 1 ° C.

(2) Experimental results

[Table 4] shows the results of the growth of Muller mushroom on the solid medium.

turn Solid medium Composition ratio (%) Growth state One Tulip sawdust 80, rice bran 15, bran 5, small amounts of CaCO _{3 and} small amounts of KH ₂ PO ₄ + 2 Oak sawdust 80, rice bran 15, wheat bran 5, small amount of CaCO _{3 and} small amount of KH ₂ PO ₄ + 3 Oak fermentation sawdust 80, rice bran 15, bran 5, small amounts of CaCO _{3 and} small amounts of KH ₂ PO ₄ ++ 4 Oak fermented sawdust 60, tulip tree fermented sawdust 25, rice bran 15, small amounts of CaCO _{3 and} small amounts of KH ₂ PO ₄ +++ 5 Oak fermented sawdust 60, tulip tree fermented sawdust 25, rice bran 15, a small amount of CaCO _{3, a} small amount of KH ₂ PO ₄ and a small amount of NH ₄ Cl +++

Based on the results of Table 4, the growth of Morel mushroom Wonjongbun was relatively better in the oak fermented sawdust medium than in the tulipwood sawdust medium or oak sawdust medium, and the oak fermented sawdust and the tulipwood sawdust had a weight ratio of 60:25 The results are shown in Fig.

FIG. 9 is a photograph showing growth rates of oak fermented sawdust and other additives in different weight ratios. (On the left, No. 1 medium - oak fermentation sawdust 85: rice bran 15/2 medium - oak fermentation sawdust 32.5: oak sawdust 52.5: rice bran 15/3 medium - oak fermentation sawdust 32.5: poplar wood sawdust 52.5: rice bran 15)

No mycelial growth of myrtle mushroom was observed in medium No. 1, and mycelial growth of myrrh mushroom was slight to the extent that it was not easy to be visually recognized in medium No. 2.

In medium No. 3, the hyphae of Morel mushroom were grown enough to be visually distinguishable.

Thus, it can be concluded that the growth efficiency of the morel mushroom cultivated by the culture method of the present invention is excellent.

Experimental Example  5. Depending on the liquid medium composition Morel mushroom  Comparison of mycelial growth rate

(1) Experimental process

In order to examine the culture method that can most effectively grow the Mycelium myrtle mushroom mycelium in the mycelial culture, it was prepared by the process of manufacturing the culture solution as described in Example 1, but the composition components such as sugar, MgSO ₄ , KH ₂ PO ₄ , peptone, Five different cultures were prepared, and the diameter and density of mycelial mushroom mycelium grown in each culture were measured to determine the growth rate of the mycelium according to each culture.

Each culture broth was sterilized at 121 ° C for 30 minutes and 5 g of each of the morel mushroom cultivated in accordance with the procedure described in Experimental Example 4 was inoculated in 500 ml of each culture solution under the conditions of aeration rate of 0.1 to 0.4 vvm and stirring speed of 50 to 300 rpm And cultured for 5 to 7 days in a deep pear culture. At this time, shaking was performed simultaneously.

(2) Experimental results

[Table 5] shows the results of growth of Morel mushroom mycelium depending on the liquid medium.

turn Culture composition Composition ratio (g / 500 mL) condition One 10 g of sugar, 2 g of MgSO ₄ , ₁ g of KH ₂ PO ₄ and 1 g of peptone + 2 15g sugar, soybean meal 1g, 2.5g MgSO _4, KH ₂ PO ₄ and 1.5g peptone 1g + 3 20 g of sugar, 3 g of soybean meal, 2.5 g of MgSO ₄ , 1.5 g of KH ₂ PO ₄ and 1 g of peptone ++ 4 15g sugar, soybean meal _{3g, MgSO 4 2.5g, KH 2} PO 4 1.5g and 1g peptone ++ 5 20 g of sugar, 3 g of soybean meal, 2.5 g of MgSO ₄ , 1.5 g of KH ₂ PO ₄ , 1 g of peptone,
1 ml of the anti-foam (204) and a small amount of NH ₄ Cl +++

Based on the results of Table 5, the mycelial mushroom mycelium showed excellent growth in medium containing 15 g of sugar and 3 g of soybean meal. In particular, 20 g of sugar, 3 g of soybean meal, 2.5 g of MgSO ₄ , 1.5 g of KH ₂ PO ₄ , g, anti - foam 1 ml and NH ₄ Cl, respectively.

After 7 days of culture in the medium, morel mushroom mycelium was obtained with a high yield of 16.7 g / L.

10 to 11 are photographs of spores of Morel mushroom mycelium observed under a microscope.

As a result of the observation, it was confirmed that the spores of the morel mushroom mycelium were excellent in activity.

Thus, it can be concluded that the growth efficiency of the mycelial mushroom mycelium cultured by the culturing method of the present invention is excellent.

Claims (5)

delete Culturing a morchella esculenta seedling bacterium in a seedling culture medium;
Culturing the cultured morel mushroom seedlings on a solid medium to obtain morel mushroom fungus; And
And culturing the morel mushroom on a liquid medium to obtain Morel mushroom mycelium,
Wherein the medium comprises a mixture of 900 to 1100 parts by weight of water, 400 to 600 parts by weight of potato extract, 10 to 30 parts by weight of dextrose, 10 to 30 parts by weight of agar, and 0.5 to 1.5 parts by weight of peptone,
The solid medium comprises 50 to 70 parts by weight of oak fermentation sawdust, 20 to 30 parts by weight of tulipwood fermented sawdust, and 10 to 20 parts by weight of rice bran,
The liquid medium comprises 15 to 25 parts by weight of sugar, 2 to 5 parts by weight of soybean meal, 2 to 3 parts by weight of MgSO ₄ , 1 to 2 parts by weight of KH ₂ PO ₄ , 0.5 to 1.5 parts by weight of peptone and 0.5 to 1.5 parts by weight of anti- Wherein the mushroom is cultivated in an amount of 1 to 20% by weight.
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