CN102964156A - Culture medium formula and preparation method of Pleurotus nebrodensis liquid spawn - Google Patents
Culture medium formula and preparation method of Pleurotus nebrodensis liquid spawn Download PDFInfo
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- CN102964156A CN102964156A CN201210316713XA CN201210316713A CN102964156A CN 102964156 A CN102964156 A CN 102964156A CN 201210316713X A CN201210316713X A CN 201210316713XA CN 201210316713 A CN201210316713 A CN 201210316713A CN 102964156 A CN102964156 A CN 102964156A
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Abstract
The invention relates to the bioengineering field, in particular to a culture medium and a preparation method thereof. According to a Pleurotus nebrodensis liquid spawn culture medium formula, the Pleurotus nebrodensis liquid spawn culture medium includes raw materials and an aqueous solution. The aqueous solution comprises the following raw materials by weight: 0.1-0.5% of yeast powder, 1-2% of sugar, 0.2-1% of bean flour, 0.05-0.1% of monopotassium phosphate, and 0.05-0.1% of magnesium sulfate. By adopting the technical scheme, the survival rate of Pleurotus nebrodensis hyphae can be improved, and the Pleurotus nebrodensis hyphae can be endowed with the characteristics of rapid growth, strong vitality, good stability, strong stress resistance after inoculation planting, difficult aging, and quick germination.
Description
Technical field
The present invention relates to a kind of bioengineering field, particularly a kind of substratum and preparation method thereof.
Background technology
Pleurotus nebrodensis is all very high edible mushroomss of a kind of edible and pharmaceutical use.The mushroom body colour pool of Pleurotus nebrodensis is pure white, meat is fine and smooth, delicious flavour, is described as " bolete on the grassland ", quite is subjected to human consumer's favor.
Pleurotus ferulae nutritious abundant, to measure according to science, its protein content accounts for 20% of dried mushroom, contains 17 seed amino acids, multivitamin and inorganic salt.Pleurotus nebrodensis also has certain pharmaceutical value, and the effects such as long-pending, the desinsection of disappearing, antibechic, anti-inflammatory and control gynecological tumor are arranged.It also contains physiologically active substance and the several mineral materials such as fungus polysaccharide and VITAMIN, has the balance the body physiological equilibrium, strengthens the effect of immune function of human body.
Pleurotus nebrodensis is produced used bacterial classification solid spawn and liquid spawn, and the relative solid spawn of liquid spawn has that cost is low, incubation time short, send out fast, the advantages such as biological transformation ratio is high, bacterial classification purity height of bacterium, is the trend of mushroom industry batch production technical renovation.And liquid spawn mainly relies on the nutrient of liquid-absorbing substratum to grow.Present existing Pleurotus nebrodensis liquid spawn culture medium and preparation method exist be prepared into that power is low, mycelial growth inadaptable, the operation professional technique require high, do not possess the shortcoming such as versatility, can't popularize in enormous quantities use.
Summary of the invention
The object of the invention is to, a kind of Pleurotus nebrodensis liquid spawn culture medium prescription is provided, solve above technical problem.
Another object of the present invention is to, the preparation method of a kind of Pleurotus nebrodensis liquid spawn is provided, solve above technical problem.
Technical problem solved by the invention can realize by the following technical solutions:
Pleurotus nebrodensis liquid spawn culture medium prescription, comprise raw material, the aqueous solution, it is characterized in that, comprise the raw material of following weight ratio in the described aqueous solution: yeast powder 0.1-0.5%, sugar 1-2%, bean powder 0.2-1%, potassium primary phosphate 0.05-0.1%, sal epsom 0.05-0.1%.
The present invention adopts the culture medium raw material of said components, and raw material sources are abundant, cheap, and the Pleurotus ferulae mycelium growth of adopting the present invention to turn out is fast, energetic, hyphae content is high, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.In addition, bean powder is grown as Pleurotus nebrodensis and is used nitrogen source, and mycelial growth is quick, sturdy.
Described raw material also comprises peptone 0.2-2%.Peptone improves the mycelial growth of Pleurotus nebrodensis also as a kind of nitrogen source.
Described raw material can also comprise at least a among wheat bran 0.3-0.5%, the Semen Maydis powder 0.3-0.5%.
Described sugar can adopt sucrose, also can adopt at least a or multiple in glucose, maltose or the white sugar.The preferred sucrose that adopts, relative other sugars, sucrose is more suitable for the carbon source as Pleurotus nebrodensis, the reaction between described sucrose and the yeast powder, the reaction between glucose, maltose and the yeast powder is more stable relatively, and cost is more cheap, utilizes effect better.
Described raw material preferably includes following weight ratio combination: yeast powder 0.2%, sugar 2%, bean powder 1%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 1%.
Described raw material also comprises at least a among following density fractions: VITAMIN 0-0.5% or the folic acid 0-0.5%.
Described VITAMIN adopts at least a in vitamins B 1 or the Lin Suanna Vitamin B2 Sodium Phosphate, and VITMAIN B1, Lin Suanna Vitamin B2 Sodium Phosphate can promote the growth of Pleurotus nebrodensis mycelia.
Folic acid is as the required nitrogenous source of a kind of Pleurotus nebrodensis mycelia growth, and an amount of folic acid can promote the growth of Pleurotus nebrodensis mycelia.
The preparation method of Pleurotus nebrodensis liquid spawn is characterized in that, comprises the steps:
1) substratum configuration: with the sugar in the raw material, sal epsom, potassium primary phosphate abundant stirring soluble in water, until be dissolved into semi-finished product solution; Again the bean powder in the described raw material, peptone, yeast powder are added stirring and dissolving in the described semi-finished product solution, and remove bubble, make medium liquid.
2) substratum adjustment: the pH value of adjusting described medium liquid with the PH conditioning agent.
3) medium sterilization: described medium liquid is poured in the seeding tank, sent into the sterilization of carrying out in the sterilizing device at least 30 minutes, in the situation of not losing nutrition, kill the bacterium in the described medium liquid.
4) inoculation: after sterilization is finished, culture medium solution is cooled to 20-25 ℃, behind the access Pleurotus nebrodensis strain, described culture temperature is adjusted to 20-23 ℃ after, described culture medium solution is carried out aerobic cultivates, can make Pleurotus nebrodensis strain in fermentation culture 7-8 days.
In the step 1), can also add at least a in raw material wheat bran, Semen Maydis powder, VITAMIN, the folic acid, can in described semi-finished product solution, add with raw materials such as bean powderes.
Step 2) in, the pH value of described medium liquid is adjusted to 6.0-6.5.
In the step 3), to described medium liquid sterilization the time, keep 123 ℃ of temperature, pressure is that 1.2 standard atmospheres are depressed sterilization.Under high-temperature high-pressure state, can kill the microorganism in the described medium liquid fully, avoid the Pleurotus nebrodensis mycelia contaminated in cultivating process.
Beneficial effect: because the employing technique scheme, the present invention can improve the survival rate of Pleurotus nebrodensis mycelia, makes fast, energetic, the good stability of Pleurotus nebrodensis mycelia growth, and after the inoculation plantation, the mycelia strong stress resistance is difficult for aging, but Fast Growth is sprouted.
Description of drawings
Fig. 1 is preparation method's of the present invention schema.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, further set forth the present invention below in conjunction with concrete diagram.
Pleurotus nebrodensis liquid spawn culture medium prescription comprises raw material, the aqueous solution, comprises the raw material of following weight ratio in the aqueous solution: yeast powder 0.1-0.5%, sugar 1-2%, bean powder 0.2-1%, potassium primary phosphate 0.05-0.1%, sal epsom 0.05-0.1%.The present invention adopts the culture medium raw material of said components, and raw material sources are abundant, cheap, and the Pleurotus ferulae mycelium growth of adopting the present invention to turn out is fast, energetic, hyphae content is high, and postvaccinal bacterial classification germination rate is good, fermentation period is short, yield rate is high.In addition, bean powder is grown as Pleurotus nebrodensis and is used nitrogen source, and mycelial growth is quick, sturdy.
Raw material also comprises peptone 0.2-2%.Peptone improves the mycelial growth of Pleurotus nebrodensis also as a kind of nitrogen source.Raw material can also comprise at least a among wheat bran 0.3-0.5%, the Semen Maydis powder 0.3-0.5%.
Sugar can adopt sucrose, also can adopt glucose, maltose or white sugar, the preferred sucrose that adopts, relative other sugars, sucrose is more suitable for the carbon source as Pleurotus nebrodensis, the reaction between sucrose and the yeast powder, and the reaction between glucose, maltose and the yeast powder is more stable relatively, cost is more cheap, utilizes effect better.
Raw material preferably includes following weight ratio combination: yeast powder 0.2%, sugar 2%, bean powder 1%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 1%.
Raw material also comprises following density fractions: VITAMIN 0-0.5%, folic acid 0-0.5%.VITAMIN adopts at least a in VITMAIN B1 or the Lin Suanna Vitamin B2 Sodium Phosphate, and VITMAIN B1, Lin Suanna Vitamin B2 Sodium Phosphate can promote the growth of Pleurotus nebrodensis mycelia.Folic acid is as the required nitrogenous source of a kind of Pleurotus nebrodensis mycelia growth, and an amount of folic acid can promote the growth of Pleurotus nebrodensis mycelia.
With reference to Fig. 1, the preparation method of Pleurotus nebrodensis liquid spawn comprises the steps:
The first step, substratum configuration: with the sugar in the raw material, sal epsom, potassium primary phosphate abundant stirring soluble in water, until be dissolved into semi-finished product solution; Again the bean powder in the raw material, peptone, yeast powder are added stirring and dissolving in the semi-finished product solution, and remove bubble, make medium liquid.Can also add at least a in raw material wheat bran, Semen Maydis powder, VITAMIN, the folic acid, can in semi-finished product solution, add with raw materials such as bean powderes, also can dissolve with sugar.
Second step, substratum adjustment: the pH value of adjusting medium liquid with the PH conditioning agent.It is good that the pH value of medium liquid is adjusted to 6.0-6.5.
The 3rd step, medium sterilization: medium liquid is poured in the seeding tank, sent into the sterilization of carrying out in the sterilizing device at least 30 minutes, in the situation of not losing nutrition, kill the bacterium in the medium liquid.When medium liquid is sterilized, keep 123 ℃ of temperature, pressure is that 1.2 standard atmospheres are depressed sterilization.Under high-temperature high-pressure state, can better remove the bacterium in the medium liquid, avoid the Pleurotus nebrodensis mycelia contaminated in cultivating process.
The 4th step, inoculation: after sterilization is finished, culture medium solution is cooled to 20-25 ℃, behind the access Pleurotus nebrodensis strain, culture temperature is adjusted to 20-23 ℃ after, culture medium solution is carried out aerobic cultivates, can make Pleurotus nebrodensis strain in fermentation culture 7-8 days.
Bean powder, peptone are as the holding effect nitrogenous source, and yeast powder is as quick-acting nitrogenous sources, and the growth that can be white jade mushroom mycelia provides enough nitrogen nutriment, is conducive to the mycelia Fast Growth.And sugar, Semen Maydis powder are as the carbon source promotion mycelial growth that can match with nitrogenous source.
When actually operating, can be on the basis of raw material preferably combination, fall into VITAMIN and folic acid and suitably adjust.
Embodiment 1:
Raw material comprises following weight ratio combination: yeast powder 0.1%, sucrose 1%, bean powder 0.2%, potassium primary phosphate 0.05%, sal epsom 0.05%, peptone 0.2%, VITAMIN 0.1%.
With the according to the above ratio abundant stirring soluble in water of the sucrose in the raw material, sal epsom, potassium primary phosphate, VITAMIN, until be dissolved into semi-finished product solution; Bean powder in the raw material, peptone, yeast powder add stirring and dissolving in the semi-finished product solution according to the above ratio again, and remove bubble, make medium liquid.
With hydrochloric acid medium liquid being carried out PH to adjusting, make its pH value be controlled at 6, keep slightly acidic, so that the Pleurotus nebrodensis growth.Then will modulate complete medium liquid and put into seeding tank, and seeding tank be put into sterilizing device sterilized 30 minutes, and during sterilization, keep 123 ℃ of temperature, pressure is that 1.2 standard atmospheres are depressed sterilization.Under high-temperature high-pressure state, can better remove the bacterium in the medium liquid, avoid the Pleurotus nebrodensis mycelia contaminated in cultivating process.After sterilization is finished, culture medium solution is cooled to 20-25 ℃, behind the access Pleurotus nebrodensis strain, culture temperature is adjusted to 20-23 ℃ after, culture medium solution is carried out aerobic cultivates, fermentation culture can make Pleurotus nebrodensis strain in 7 days.
Embodiment 2:
Raw material comprises following weight ratio combination: yeast powder 0.5%, glucose 2%, bean powder 1%, potassium primary phosphate 0.1%, sal epsom 0.1%, peptone 1%, VITAMIN 0.5%, folic acid 0.5%.
With the glucose in the raw material, sal epsom, potassium primary phosphate abundant stirring soluble in water, until be dissolved into semi-finished product solution; Again the bean powder in the raw material, peptone, yeast powder, VITAMIN, folic acid are added stirring and dissolving in the semi-finished product solution, and remove bubble, make medium liquid.Use is adjusted the pH value of medium liquid the harmless PH conditioning agent of mushroom, and the pH value that makes medium liquid is 6.5.Then medium liquid is being poured into seeding tank, putting into sterilizing device and carry out 40 minutes sterilization, in the situation of not losing nutrition, killing the bacterium in the medium liquid.When medium liquid is sterilized, keep 123 ℃ of temperature, pressure is that 1.2 standard atmospheres are depressed sterilization.Under high-temperature high-pressure state, can better remove the bacterium in the medium liquid, avoid the Pleurotus nebrodensis mycelia contaminated in cultivating process.After sterilization is finished, culture medium solution is cooled to below 25 ℃, behind the access Pleurotus nebrodensis strain, culture temperature is adjusted to 20-23 ℃ after, culture medium solution is carried out aerobic cultivates, fermentation culture can make Pleurotus nebrodensis strain in 8 days.
Embodiment 3:
Raw material comprises following weight ratio combination: yeast powder 0.3%, sugar 1.5%, bean powder 0.6%, potassium primary phosphate 0.08%, sal epsom 0.07%, peptone 0.4%, VITAMIN 0.2%, folic acid 0.3%.
With the glucose in the raw material, sal epsom, potassium primary phosphate abundant stirring soluble in water, until be dissolved into semi-finished product solution; Again the bean powder in the raw material, peptone, yeast powder, VITAMIN, folic acid are added stirring and dissolving in the semi-finished product solution, and remove bubble, make medium liquid.Use is adjusted the pH value of medium liquid the harmless PH conditioning agent of mushroom, and the pH value that makes medium liquid is 6-6.5.Then medium liquid is being poured into seeding tank, putting into the sterilization that sterilizing device carried out 30 minutes at least, in the situation of not losing nutrition, killing the bacterium in the medium liquid.When medium liquid is sterilized, keep 123 ℃ of temperature, pressure is that 1.2 standard atmospheres are depressed sterilization.Under high-temperature high-pressure state, can better remove the bacterium in the medium liquid, avoid the Pleurotus nebrodensis mycelia contaminated in cultivating process.After sterilization is finished, culture medium solution is cooled to below 25 ℃, behind the access Pleurotus nebrodensis strain, culture temperature is adjusted to 20-23 ℃ after, culture medium solution is carried out aerobic cultivates, fermentation culture can make Pleurotus nebrodensis strain in 7 days.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that describes in above-described embodiment and the specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (9)
1. the Pleurotus nebrodensis liquid spawn culture medium is filled a prescription, comprise raw material, the aqueous solution, it is characterized in that, comprise the raw material of following weight ratio in the described aqueous solution: yeast powder 0.1-0.5%, sugar 1-2%, bean powder 0.2-1%, potassium primary phosphate 0.05-0.1%, sal epsom 0.05-0.1%.
2. Pleurotus nebrodensis liquid spawn culture medium according to claim 1 is filled a prescription, and it is characterized in that: described raw material also comprises peptone 0.2-2%.
3. Pleurotus nebrodensis liquid spawn culture medium according to claim 1 prescription is characterized in that: described raw material also comprises at least a among wheat bran 0.3-0.5%, the Semen Maydis powder 0.3-0.5%.
4. Pleurotus nebrodensis liquid spawn culture medium according to claim 1 prescription is characterized in that: described sugar adopts at least a in sucrose, glucose, maltose or the white sugar.
5. the described Pleurotus nebrodensis liquid spawn culture medium prescription of any one in 5 according to claim 1 is characterized in that: described raw material also comprises at least a among following density fractions: VITAMIN 0-0.5% or the folic acid 0-0.5%.
6. the preparation method of Pleurotus nebrodensis liquid spawn is characterized in that, comprises the steps:
1) substratum configuration: with the sugar in the raw material, sal epsom, potassium primary phosphate abundant stirring soluble in water, until be dissolved into semi-finished product solution; Again the bean powder in the described raw material, peptone, yeast powder are added stirring and dissolving in the described semi-finished product solution, and remove bubble, make medium liquid;
2) substratum adjustment: the pH value of adjusting described medium liquid with the PH conditioning agent;
3) medium sterilization: described medium liquid is poured in the seeding tank, sent into the sterilization of carrying out in the sterilizing device at least 30 minutes, in the situation of not losing nutrition, kill the bacterium in the described medium liquid;
4) inoculation: after sterilization is finished, culture medium solution is cooled to 20-25 ℃, behind the access Pleurotus nebrodensis strain, described culture temperature is adjusted to 20-23 ℃ after, described culture medium solution is carried out aerobic cultivates, can make Pleurotus nebrodensis strain in fermentation culture 7-8 days.
7. the preparation method of Pleurotus nebrodensis liquid spawn according to claim 6 is characterized in that: in the step 1), also add at least a in raw material wheat bran, Semen Maydis powder, VITAMIN, the folic acid.
8. the preparation method of Pleurotus nebrodensis liquid spawn according to claim 6 is characterized in that: step 2) in, the pH value of described medium liquid is adjusted to 6-6.5.
9. the preparation method of Pleurotus nebrodensis liquid spawn according to claim 6 is characterized in that: in the step 3), to described medium liquid sterilization the time, keep 123 ℃ of temperature, pressure is that 1.2 standard atmospheres are depressed sterilization.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104041818A (en) * | 2014-06-26 | 2014-09-17 | 河南省菇业公社食品有限公司 | Making method of pleurotus nebrodensis mycelium chewable tablet |
| CN104261942A (en) * | 2014-09-28 | 2015-01-07 | 永和县旺达食用菌有限责任公司 | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain |
| CN110129205A (en) * | 2018-02-09 | 2019-08-16 | 鲁东大学 | A kind of Pleurotus nebrodensis liquid spawn culture medium and Pleurotus nebrodensis liquid spawn preparation method |
| CN111034539A (en) * | 2018-10-15 | 2020-04-21 | 于智勇 | Liquid culture medium for edible fungi |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041818A (en) * | 2014-06-26 | 2014-09-17 | 河南省菇业公社食品有限公司 | Making method of pleurotus nebrodensis mycelium chewable tablet |
| CN104261942A (en) * | 2014-09-28 | 2015-01-07 | 永和县旺达食用菌有限责任公司 | Pleurotus nebrodensis liquid strain culture medium and propagation method of strain |
| CN110129205A (en) * | 2018-02-09 | 2019-08-16 | 鲁东大学 | A kind of Pleurotus nebrodensis liquid spawn culture medium and Pleurotus nebrodensis liquid spawn preparation method |
| CN111034539A (en) * | 2018-10-15 | 2020-04-21 | 于智勇 | Liquid culture medium for edible fungi |
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Application publication date: 20130313 |