CN102964170A - Formula and preparation method of agaricus bisporus liquid spawn - Google Patents
Formula and preparation method of agaricus bisporus liquid spawn Download PDFInfo
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- CN102964170A CN102964170A CN2012103163425A CN201210316342A CN102964170A CN 102964170 A CN102964170 A CN 102964170A CN 2012103163425 A CN2012103163425 A CN 2012103163425A CN 201210316342 A CN201210316342 A CN 201210316342A CN 102964170 A CN102964170 A CN 102964170A
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Abstract
The invention relates to the field of bioengineering, in particular to a culture medium and a preparation method thereof. According to a formula, an agaricus bisporus liquid spawn includes raw materials and a water solution. The raw materials are contained in the water solution and are composed of the following components by weight: 0.1-1.0% of yeast powder, 1.5-3% of sugar, 0.05-0.3% of potassium dihydrogen phosphate, 0.05-0.3% of magnesium sulfate, and 0.1-0.5% of peptone. The preparation method of the agaricus bisporus liquid spawn comprises the steps of: culture medium preparation, culture medium adjustment, culture medium sterilization, and mycelium inoculation. By adopting the technology, the agaricus bisporus mycelia cultivated by the invention have the advantages of vigorous vitality, difficult aging, rapid spawn running speed, and strong disease resistance, thus being easy for large-scale planting.
Description
Technical field
The present invention relates to a kind of bioengineering field, particularly a kind of substratum and preparation method thereof.
Background technology
Edible mushrooms is the food that is of high nutritive value that people like, and the edible mushrooms artificial cultivation technique has very large development in recent years.Pleurotus eryngii, mushroom, bisporous mushroom etc. are arranged in the edible mushrooms.Wherein, the bisporous mushroom meat is fertile, delicious in taste, mouthfeel is abundant, extensively is subjected to people's favor.And in the process of cultivating bisporous mushroom, can relate to a lot of links, wherein the mycelium culture of bisporous mushroom is one of important step of bisporous mushroom cultivation.
In the production process of bisporous mushroom; bisporous mushroom is produced used bacterial classification solid spawn and liquid spawn; the relative solid spawn of liquid spawn has short, fast, the advantage such as biological transformation ratio is high, bacterial classification purity height of bacterium of incubation time; and the mass-producing of being more convenient for, continuously production are the trend of mushroom industry batch production method cane technical renovation.But, at present the liquid spawn culture medium of bisporous mushroom exists and is prepared into that power is low, mycelial growth inadaptable, the problem that occurs easily the aging regression of mycelia in late stage of culture, and the operation professional technique requires high, in large-scale production process, can directly have influence on efficient and its economic benefits that bisporous mushroom is cultivated.
Summary of the invention
The object of the invention is to, a kind of bisporous mushroom liquid spawn prescription is provided, solve above technical problem.
Another object of the present invention is to, the preparation method of a kind of bisporous mushroom liquid spawn is provided, solve above technical problem.
Technical problem solved by the invention can realize by the following technical solutions:
Bisporous mushroom liquid spawn prescription, comprise raw material, the aqueous solution, it is characterized in that the weight percent of described raw material in the described aqueous solution is as follows: yeast powder 0.1-1.0%, sugar 1.5-3%, potassium primary phosphate 0.05-0.3%, sal epsom 0.05-0.3%, peptone 0.1-0.5%.
Among the present invention, adopt yeast powder as the required major nitrogen source of bisporous mushroom liquid mycelial growth, described sugar is as main carbon source, peptone then both can be used as nitrogenous source and also can be used as carbon source, in bisporous mushroom liquid mycelia process of growth, can play and regulate the effect that replenishes, and the growth that is added to bisporous mushroom of potassium primary phosphate and sal epsom provides necessary trace element, guarantee the nutriment that bisporous mushroom liquid mycelia can enough be enriched, have more vitality, strengthen the resistance against diseases of mycelia.
Described sugar can adopt sucrose, also can adopt glucose or maltose, the preferred sucrose that adopts, relative other sugars, sucrose is more suitable for the carbon source as bisporous mushroom, the reaction between described sucrose and the yeast powder, and the reaction between glucose, maltose and the yeast powder is more stable relatively, cost is more cheap, utilizes effect better.
The raw material that comprises following weight percent in the described aqueous solution: yeast powder 0.2%, sucrose 2%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 0.3%.
Described raw material also comprises potato powder; The raw material that contains following weight ratio in the described aqueous solution: potato powder 0-1.0%.
The raw material that comprises following weight percent in the described aqueous solution: yeast powder 0.3%, sucrose 2.5%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 0.2%, potato powder 0.5%.
Said components is mostly originated abundant, and is cheap, is convenient to a large amount of uses, carries out volume production.And the bisporous mushroom mycelia of adopting the present invention to cultivate, mycelial growth is quick, and is energetic, and mycelial density is higher in same sprout time.
The raw material that also comprises following proportion in the described aqueous solution: soybean peptides 0.05-0.5%, calcium sulfate 0.01-0.1%.The described soybean peptides of suitable adding is as nitrogenous source, and the growth that can be described bisporous mushroom provides enough nutriment.And in the process of bisporous mushroom growth, the growth that needs the calcium constituent of trace, the interpolation of described calcium sulfate to can be bisporous mushroom provides the nutritive ingredient that needs.
The weight percent of described raw material in the described aqueous solution is as follows: yeast powder 0.3%, sucrose 2.5%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 0.2%, potato powder 0.5%, soybean peptides 0.2%, calcium sulfate 0.03%.
The preparation method of bisporous mushroom liquid spawn is characterized in that, comprises the steps:
1) substratum configuration: raw material is poured in the aqueous solution in proportion, and removed bubble after fully stirring, make medium liquid.
2) substratum adjustment: the pH value to described medium liquid is tested, and adjusts the pH value of described medium liquid with the PH conditioning agent, makes its pH value keep 6.5-7.0.
3) medium sterilization: described medium liquid is put into sterilizing device carried out high-temperature sterilization at least 15 minutes, the described medium liquid that then will be in high temperature carries out stirring with the stirring velocity of 150-200rpm under aseptic condition.
4) bacterial classification inoculation: medium liquid is cooled off, after making the temperature of described medium liquid remain on 20-25 ℃, access bisporous mushroom bacterial classification, the medium liquid that will contain the bisporous mushroom bacterial classification carries out aerobic to be cultivated 7-10 days by purifying air, and can make the bisporous mushroom bacterial classification of high-quality.
In the step 1), the weight percent of described raw material in the described aqueous solution is as follows: yeast powder 0.1-1.0%, sugar 1.5-3%, potassium primary phosphate 0.05-0.3%, sal epsom 0.05-0.3%, peptone 0.1-0.5%.
Step 2) in, described PH conditioning agent can adopt at least a in dipotassium hydrogen phosphate, the potassium primary phosphate.Described PH conditioning agent preferably adopts dipotassium hydrogen phosphate, and it is relatively acid that the raw material of described medium liquid contains potassium primary phosphate, can its pH value be in harmonious proportion as neutral by adding described dipotassium hydrogen phosphate.
In the step 3), when aerobic was cultivated, keeping the temperature in the described culturing room was 20-23 ℃, and the medium liquid that contains described bisporous mushroom bacterial classification is carried out shaking culture.
Beneficial effect: after adopting above-mentioned technology, the bisporous mushroom mycelia that the present invention cultivates, mycelia vitality is vigorous, is difficult for aging, and it is fast to send out bacterium speed, and resistance against diseases is strong, easily implant mass.
Description of drawings
Fig. 1 is preparation method's of the present invention schema.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, further set forth the present invention below in conjunction with concrete diagram.
With reference to Fig. 1, bisporous mushroom liquid spawn prescription comprises raw material, the aqueous solution, and the weight percent of raw material in the aqueous solution is as follows: yeast powder 0.1-1.0%, sugar 1.5-3%, potassium primary phosphate 0.05-0.3%, sal epsom 0.05-0.3%, peptone 0.1-0.5%.Sugar can adopt sucrose, also can adopt glucose or maltose, the preferred sucrose that adopts, relative other sugars, sucrose is more suitable for the carbon source as bisporous mushroom, the reaction between sucrose and the yeast powder, and the reaction between glucose, maltose and the yeast powder is more stable relatively, cost is more cheap, utilizes effect better.The raw material that comprises following weight percent in the aqueous solution: yeast powder 0.2%, sucrose 2%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 0.3%.
Raw material also comprises potato powder 0-1.0%.The raw material that comprises following weight percent in the aqueous solution: yeast powder 0.2%, sucrose 2%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 0.3%, potato powder 0.5%.The said components component is mostly originated abundant, and is cheap, is convenient to a large amount of uses, carries out volume production.And the bisporous mushroom mycelia of adopting the present invention to cultivate, mycelial growth is quick, and is energetic, and mycelial density is higher in same sprout time.
Raw material also comprises following density fractions: soybean peptides 0.05-0.5%, calcium sulfate 0.01-0.1%.Suitable adding soybean peptides is as nitrogenous source, and the growth that can be bisporous mushroom provides enough nutriment.And in the process of bisporous mushroom growth, the growth that needs the calcium constituent of trace, the interpolation of calcium sulfate to can be bisporous mushroom provides the nutritive ingredient that needs.The weight percent of raw material in the described aqueous solution is as follows: yeast powder 0.3%, sucrose 2.5%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 0.2%, potato powder 0.5%, soybean peptides 0.2%, calcium sulfate 0.03%.
Embodiment 1:
20g sucrose, 0.8g sal epsom, 0.8g potassium primary phosphate poured in 1000 milliliters the aqueous solution and stir, until be dissolved into work in-process; Again 3g yeast powder, 5g potato powder, 3g peptone are put into work in-process, remove bubble after fully stirring, make medium liquid.PH value to medium liquid is tested, and adjusts the pH value of medium liquid with dipotassium hydrogen phosphate, makes its pH value keep 6.5-7.0.
Medium liquid is put into sterilizing device, and to carry out sterilising temp be 121-123 ℃, and air pressure is the high-temperature sterilization of 1.3 standard atmospheric pressures, during high-temperature sterilization, keeps at least 15 minutes high-temperature sterilization state.After the stirring velocity with 150-200rpm of the medium liquid that then will be in high temperature carrying out under aseptic condition stirs, medium liquid is cooled off, after making the temperature of medium liquid remain on 20-21.9 ℃, access bisporous mushroom bacterial classification, the medium liquid that will contain the bisporous mushroom bacterial classification carries out aerobic and cultivated 7-10 days by purifying air, when aerobic was cultivated, keeping the temperature in the culturing room was 20-23 ℃, and the medium liquid that contains the bisporous mushroom bacterial classification is carried out shaking culture.
Embodiment 2:
15g sucrose, 10g maltose, 0.8g sal epsom, 0.8g potassium primary phosphate, 3g yeast powder, 3g peptone poured into together in 1000 milliliters the aqueous solution, and after fully stirring with stirrer, remove bubble.
Utilize the PH test paper that medium liquid is carried out pH value test, with dipotassium hydrogen phosphate and potassium primary phosphate the pH value of medium liquid is debugged and be neutrality.Then medium liquid is put into sterilizing device and carried out autoclave sterilization 30 minutes, during sterilization, the temperature of sterilizing is transferred to 124-125 ℃, air pressure is 1.3 standard atmospheric pressures.
Sterilize complete after, after under sterile state, taking out medium liquid and it being cooled to 22-23.9 ℃, as above-mentioned embodiment 1, inoculate and aerobic is cultivated.
Embodiment 3:
With 25g glucose, 0.8g sal epsom, 0.8g potassium primary phosphate, pour in 1000 milliliters the aqueous solution, and stirring is dissolved it, then put into 3g yeast powder, 5g potato powder, 3g peptone, 3g soybean peptides, fully stirring by stirrer becomes medium liquid, and medium liquid is removed bubble.And with the PH tester medium liquid is carried out pH value test, with hydrochloric acid and sodium hydroxide the pH value of medium liquid is transferred to 6.5-7.0.
Then medium liquid is put into the ultrasonic sterilization device carry out disinfection the sterilization at least 30 minutes, then after medium liquid being cooled to 24-25 ℃, access bisporous mushroom bacterial classification, the medium liquid that will contain the bisporous mushroom bacterial classification carries out aerobic and cultivated 7-10 days by purifying air, when aerobic is cultivated, keeping the temperature in the culturing room is 20-23 ℃, and the medium liquid that contains the bisporous mushroom bacterial classification is carried out shaking culture.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that describes in above-described embodiment and the specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (9)
1. the bisporous mushroom liquid spawn is filled a prescription, comprise raw material, the aqueous solution, it is characterized in that the weight percent of described raw material in the described aqueous solution is as follows: yeast powder 0.1-1.0%, sugar 1.5-3%, potassium primary phosphate 0.05-0.3%, sal epsom 0.05-0.3%, peptone 0.1-0.5%.
2. bisporous mushroom liquid spawn prescription according to claim 1 is characterized in that: described sugar employing sucrose.
3. bisporous mushroom liquid spawn according to claim 2 is filled a prescription, and it is characterized in that: described raw material also comprises potato powder; The raw material that contains following weight ratio in the described aqueous solution: potato powder 0-1.0%.
4. bisporous mushroom liquid spawn prescription according to claim 3 is characterized in that: the raw material that comprises following weight percent in the described aqueous solution: yeast powder 0.2%, sucrose 2%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 0.3%, potato powder 0.5%.
5. bisporous mushroom liquid spawn prescription according to claim 4 and preparation method thereof is characterized in that: the raw material that also comprises following proportion in the described aqueous solution: soybean peptides 0.05-0.5%, calcium sulfate 0.01-0.1%.
6. the preparation method of bisporous mushroom liquid spawn is characterized in that, comprises the steps:
1) substratum configuration: substratum configuration: raw material is poured in the aqueous solution in proportion, and removed bubble after fully stirring, make medium liquid;
2) substratum adjustment: the pH value to described medium liquid is tested, and adjusts the pH value of described medium liquid with the PH conditioning agent, makes its pH value keep 6.5-7.0;
3) medium sterilization: described medium liquid is put into sterilizing device carried out high-temperature sterilization at least 15 minutes, the described medium liquid that then will be in high temperature carries out stirring with the stirring velocity of 150-200rpm under aseptic condition;
4) mycelium inoculation: medium liquid is cooled off, make the temperature of described medium liquid remain on 20-25 ℃ after, access bisporous mushroom bacterial classification, the medium liquid that will contain the bisporous mushroom bacterial classification carries out aerobic to be cultivated 7-10 days, can make the bisporous mushroom bacterial classification.
7. the preparation method of bisporous mushroom liquid spawn according to claim 6, it is characterized in that: the weight percent of described raw material in the described aqueous solution is as follows: yeast powder 0.2%, sucrose 2%, potassium primary phosphate 0.08%, sal epsom 0.08%, peptone 0.3%.
8. the preparation method of bisporous mushroom liquid spawn according to claim 6 is characterized in that: step 2) in, described PH conditioning agent adopts dipotassium hydrogen phosphate.
9. the preparation method of bisporous mushroom liquid spawn according to claim 6 is characterized in that: in the step 3), when aerobic was cultivated, keeping the temperature in the described culturing room was 20-23 ℃, and the medium liquid that contains described bisporous mushroom bacterial classification is carried out shaking culture.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103782801A (en) * | 2013-12-26 | 2014-05-14 | 朝阳鑫源农副产品开发有限公司 | Agaricus Bisporus liquid spawn and preparation method thereof |
| CN104012298A (en) * | 2014-04-29 | 2014-09-03 | 潢川九龙春天农业科技有限公司 | Edible fungus liquid seed submerged fermentation packaged seed production technology and medium formulas thereof |
| CN106119124A (en) * | 2016-06-27 | 2016-11-16 | 合肥福泉现代农业科技有限公司 | A kind of Pleurotus abalonus liquid spawn culture medium based on giant knotweed rhizome residue and Pleurotus abalonus liquid spawn preparation method |
| CN108207493A (en) * | 2018-02-09 | 2018-06-29 | 山东省农业科学院农业资源与环境研究所 | A kind of grass corruption type edible fungus liquid culture growth medium, preparation method and application |
| CN108440067A (en) * | 2018-05-10 | 2018-08-24 | 习水县龙洋生态食品开发有限公司 | A kind of pholiota nameko culture medium |
| CN113692917A (en) * | 2021-08-26 | 2021-11-26 | 王以军 | Bag type cultivation method for culturing agaricus bisporus liquid strain clinker |
| CN114503873A (en) * | 2020-11-16 | 2022-05-17 | 上海国森生物科技有限公司 | Agaricus bisporus liquid strain reduction strain and cultivation method thereof |
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| CN101270370A (en) * | 2008-03-17 | 2008-09-24 | 张永浩 | Method for producing ethanol and cultivating edible mushroom with manioc waste |
| CN102442853A (en) * | 2011-03-24 | 2012-05-09 | 上海雪榕生物科技股份有限公司 | Formula of culture medium for yellow flammulina liquid strain and preparation method of culture medium |
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- 2012-08-30 CN CN2012103163425A patent/CN102964170A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101270370A (en) * | 2008-03-17 | 2008-09-24 | 张永浩 | Method for producing ethanol and cultivating edible mushroom with manioc waste |
| CN102442853A (en) * | 2011-03-24 | 2012-05-09 | 上海雪榕生物科技股份有限公司 | Formula of culture medium for yellow flammulina liquid strain and preparation method of culture medium |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782801A (en) * | 2013-12-26 | 2014-05-14 | 朝阳鑫源农副产品开发有限公司 | Agaricus Bisporus liquid spawn and preparation method thereof |
| CN104012298A (en) * | 2014-04-29 | 2014-09-03 | 潢川九龙春天农业科技有限公司 | Edible fungus liquid seed submerged fermentation packaged seed production technology and medium formulas thereof |
| CN104012298B (en) * | 2014-04-29 | 2016-05-04 | 潢川九龙春天农业科技有限公司 | The complete production of hybrid seeds technique of edible fungus liquid kind submerged fermentation and culture medium prescription thereof |
| CN106119124A (en) * | 2016-06-27 | 2016-11-16 | 合肥福泉现代农业科技有限公司 | A kind of Pleurotus abalonus liquid spawn culture medium based on giant knotweed rhizome residue and Pleurotus abalonus liquid spawn preparation method |
| CN108207493A (en) * | 2018-02-09 | 2018-06-29 | 山东省农业科学院农业资源与环境研究所 | A kind of grass corruption type edible fungus liquid culture growth medium, preparation method and application |
| CN108440067A (en) * | 2018-05-10 | 2018-08-24 | 习水县龙洋生态食品开发有限公司 | A kind of pholiota nameko culture medium |
| CN114503873A (en) * | 2020-11-16 | 2022-05-17 | 上海国森生物科技有限公司 | Agaricus bisporus liquid strain reduction strain and cultivation method thereof |
| CN113692917A (en) * | 2021-08-26 | 2021-11-26 | 王以军 | Bag type cultivation method for culturing agaricus bisporus liquid strain clinker |
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Application publication date: 20130313 |