CN109328875A - A kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch - Google Patents
A kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch Download PDFInfo
- Publication number
- CN109328875A CN109328875A CN201811306719.2A CN201811306719A CN109328875A CN 109328875 A CN109328875 A CN 109328875A CN 201811306719 A CN201811306719 A CN 201811306719A CN 109328875 A CN109328875 A CN 109328875A
- Authority
- CN
- China
- Prior art keywords
- lotus root
- root starch
- culture medium
- cordyceps militaris
- shaking flask
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention belongs to culture medium of edible fungus fields, and in particular to a kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch.The culture medium primary raw material has glucose, potassium dihydrogen phosphate, magnesium sulfate, peptone, lotus root starch, potato, water.The present invention joined lotus root starch in liquid spawn culture medium formula, accelerate mycelial growth rate, bacterium ball is tiny dense, and dry weight is big.This formula is prepared simply, for not only increasing liquid spawn quality in producing, is also shortened growth cycle, is suitable for Cordyceps militaris shaking flask strain and produces.
Description
Technical field
The invention belongs to culture medium of edible fungus field, in particular to a kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch and
Preparation method.
Background technique
Cordyceps militaris also known as Cordceps militaris, scientific name northern Chinese caterpillar Fungus, because of its chemical component and pharmacological action and cordyceps sinensis phase
Seemingly, clinically often it is used as medicine instead of cordyceps sinensis.Large-scale artificial culture and submerged fermentation, large-scale artificial training have been realized at present
It supports and provides a large amount of cordyceps militaris sporocarp for people, and raw material can be provided to extract effective active material, and people can
To pass through submerged fermentation effective component extracting directly from fermentation liquid.In cultivation practices, the process of strain is made are as follows: test tube bacterium
Kind → shaking flask strain → fermentor, the strain of fermentor can be directly used in cultivation after dilution, entire strain produce by
Grade expands numerous, it can be seen that high quality, high standard shaking flask strain be an important link in production.So-called shaking flask strain is just
It is liquid spawn, is frequently referred to second class inoculum in production.Can the quality of shaking flask strain performance be numerous to the expansion of later period strain, and cultivate
Grass has direct influence out.The present invention seeks to increase the culture medium prescription of suitable shaking flask strain to improve the matter of strain
Amount guarantees the needs of Cordyceps militaris shaking flask strain production.
The process of lotus root starch used: fresh lotus rhizome-cleaning-crushing-filter-is removed sand purification-concentration purification-
Dehydration-drying.The nutritive value of lotus root starch is very high, rich in the microelements such as iron, calcium, phytoprotein, vitamin, glucose with
And content of starch is also very abundant.By consulting a large amount of documents and materials, do not find for lotus root starch to be used for Cordyceps militaris shaking flask Spawn incubation
Report in base.
Summary of the invention
The present invention provides a kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch, this formula is exactly normal
It joined lotus root starch in the liquid formulations of rule, it is convenient to prepare, and low in cost, actual operation is simple.
To achieve the above object, the present invention adopts the following technical scheme that.
A kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, formula include following components: glucose, potassium dihydrogen phosphate,
Magnesium sulfate, peptone, lotus root starch, potato, water.
The Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphorus
Acid dihydride potassium 3g, magnesium sulfate 1.5g, peptone 3g, lotus root starch 1-7.5g, potato 200g, water 1000mL.
The Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphorus
Acid dihydride potassium 3g, magnesium sulfate 1.5g, lotus root starch 1-7.5g, potato 200g, water 1000mL.
The preparation method of the Cordyceps militaris shaking flask liquid spawn culture medium containing lotus root starch, includes the following steps.
(1) it after peeling potatoes, will first be cut into small pieces to be put into 1000mL water and be cooked, boil thoroughly well cooked but not mushy, four layers of gauze
Filter to take juice.
(2) by glucose, potassium dihydrogen phosphate, magnesium sulfate, peptone, weigh up in proportion after, be put into filtered horse in (1)
In bell potato juice, stirring evenly melts drug all, to constant volume to 900mL.
(3) it after stirring lotus root starch with 30 DEG C of 100mL or less cold water, is added in (2) and is sufficiently stirred evenly in the liquid of constant volume, point
It is not fitted into the triangular flask of 250mL, every bottle of 100mL, covers sealed membrane or rubber plug, 121 DEG C~126 DEG C high pressure sterilizations, sterilizing
30min。
Compared with the prior art, beneficial effects of the present invention.
The lotus root starch in Cordyceps militaris shaking flask bacterium culture medium in the present invention include phytoprotein, vitamin, glucose and
Starch, rich in microelements such as iron, calcium.Carbon source is to be capable of providing carbon skeleton in cordyceps mycelium composition cell and metabolite to come
The nutriment in source；Grape sugar and starch in lotus root starch can be used as the carbon source of Cordyceps militaris mycelia growth.Nitrogen source is cordyceps
The raw material of nucleic acid needed for mycelial growth and protein synthesis；Phytoprotein in lotus root starch can be used as the growth of Cordyceps militaris mycelia
Nitrogen source.Cordyceps mycelium also needs vitamin and microelement in growth and development, and the nutritional ingredient in lotus root starch also can
Meet；So lotus root starch can not only serve as carbon source, but also may act as nitrogen source and vitamin and microelement, it can directly be influenced
The biomass of cordyceps mycelium.
Strain particle using the Cordyceps militaris Shake flask medium containing lotus root starch is small, and strain amount is big, and mycelial growth rate is fast, raw
Long period is short.Hypha growth condition and full bottle time from test, while measuring bacterium ball size, Peloton density, mycelium
The indexs such as dry weight, generally effect are obvious, improve strain quality.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below.
Embodiment 1.
A kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphoric acid
Potassium dihydrogen 3g, magnesium sulfate 1.5g, peptone 3g, lotus root starch 1g, potato 200g, water 1000mL.
Cordyceps militaris shaking flask bacterium culture medium preparation method, includes the following steps.
(1) it after peeling potatoes, will first be cut into small pieces to be put into 1000mL water and be cooked, boil thoroughly well cooked but not mushy, four layers of gauze
Filter to take juice.
(2) by glucose, potassium dihydrogen phosphate, magnesium sulfate, peptone, weigh up in proportion after, be put into above-mentioned filtered horse
In bell potato juice, stirring evenly melts drug all, constant volume to 900mL.
(3) it after stirring lotus root starch with 30 DEG C of 100mL or less cold water, is added in the liquid of above-mentioned constant volume and sufficiently stirs evenly, respectively
It is fitted into the triangular flask of 250mL, every bottle of 100mL, covers sealed membrane or rubber plug, 121 DEG C~126 DEG C high pressure sterilizations, sterilizing
30min is inoculated with after cooling.
(4) Φ 6mm is taken to be inoculated in (3) in sterilized shaking flask bacterium culture medium with punch the strain after activation,
It is put into 20 DEG C of incubator half-light static gas wave refrigerators for 24 hours, observes whether bacterium solution has muddiness during half-light culture, place into 20 afterwards for 24 hours
DEG C shaking table (150r/min), mycelia is not slowly long in the training period or peculiar smell occur is contaminated bacteria bottle, the shaking flask bacterium grown
Kind carries out plate culture and microexamination to find the pollution whether there is or not bacterium and mould in time, guarantees strain quality.
Embodiment 2.
A kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphoric acid
Potassium dihydrogen 3g, magnesium sulfate 1.5g, peptone 3g, lotus root starch 2.5g, potato 200g, water 1000mL.
Cordyceps militaris shaking flask bacterium culture medium preparation method is the same as embodiment 1.
Embodiment 3.
A kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphoric acid
Potassium dihydrogen 3g, magnesium sulfate 1.5g, peptone 3g, lotus root starch 5g, potato 200g, water 1000mL.
Cordyceps militaris shaking flask bacterium culture medium preparation method is the same as embodiment 1.
Embodiment 4.
A kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphoric acid
Potassium dihydrogen 3g, magnesium sulfate 1.5g, peptone 3g, lotus root starch 7.5g, potato 200g, water 1000mL.
Cordyceps militaris shaking flask bacterium culture medium preparation method is the same as embodiment 1.
Embodiment 5.
A kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphoric acid
Potassium dihydrogen 3g, magnesium sulfate 1.5g, lotus root starch 1g, potato 200g, water 1000mL.
Cordyceps militaris shaking flask bacterium culture medium preparation method is the same as embodiment 1.
Embodiment 6.
A kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphoric acid
Potassium dihydrogen 3g, magnesium sulfate 1.5g, lotus root starch 2.5g, potato 200g, water 1000mL.
Cordyceps militaris shaking flask bacterium culture medium preparation method is the same as embodiment 1.
Embodiment 7.
A kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphoric acid
Potassium dihydrogen 3g, magnesium sulfate 1.5g, lotus root starch 5g, potato 200g, water 1000mL.
Cordyceps militaris shaking flask bacterium culture medium preparation method is the same as embodiment 1.
Embodiment 8.
A kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, raw material are as follows by weight ratio: glucose 20g, phosphoric acid
Potassium dihydrogen 3g, magnesium sulfate 1.5g, lotus root starch 7.5g, potato 200g, water 1000mL.
Cordyceps militaris shaking flask bacterium culture medium preparation method is the same as embodiment 1.
It compares conventional formulation (CK1): glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, peptone 3g, potato
200g, water 1000mL.
It compares conventional formulation (CK2): glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, potato 200g, water
1000mL。
Cordyceps militaris shaking flask bacterium culture medium preparation method is the same as embodiment 1.
As a result be analyzed as follows.
It joined different amounts of lotus root starch in formula 1-4 conventional formulation as can be seen from the table, be formulated in 5-8 and use lotus root starch generation
It joined different amounts of lotus root starch for nitrogen source peptone, be added to the culture medium of lotus root starch, Peloton density, bacterium bulb diameter, mycelia soma
Variation is obvious again.Testing sieve be comprehensively compared select that be suitable for the formula of cordyceps mycelium growth be 6 and 7, lotus root starch additive amount point
Not Wei 2.5g/L and 5g/L, both formulas are better than other culture medium prescriptions and comparative formula, and lotus root starch is matched as nitrogen source ratio
It is good that peptone effect is added in side.
Claims (4)
1. a kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, which is characterized in that formula includes following components: glucose, phosphorus
Acid dihydride potassium, magnesium sulfate, peptone, lotus root starch, potato, water.
2. a kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch as described in claim 1, which is characterized in that its raw material is by weight
Amount proportion it is as follows: glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, peptone 3g, lotus root starch 1-7.5g, potato 200g,
Water 1000mL.
3. a kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch as described in claim 1, which is characterized in that its raw material is by weight
Amount proportion is as follows: glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, lotus root starch 1-7.5g, potato 200g, water 1000mL.
4. a kind of Cordyceps militaris shaking flask bacterium culture medium containing lotus root starch, which is characterized in that the preparation method of culture medium, including following step
It is rapid:
(1) it after peeling potatoes, will first be cut into small pieces to be put into 1000mL water and be cooked, boil thoroughly well cooked but not mushy, four layers of filtered through gauze
Take juice；
(2) by glucose, potassium dihydrogen phosphate, magnesium sulfate, peptone, weigh up in proportion after, be put into filtered potato in (1)
In juice, stirring evenly melts drug all, to constant volume to 900mL；
(3) it after stirring lotus root starch with 30 DEG C of 100mL or less cold water, is added in (2) and is sufficiently stirred evenly in the liquid of constant volume, filled respectively
Enter in the triangular flask of 250mL, every bottle of 100mL, covers sealed membrane or rubber plug, 121 DEG C~126 DEG C high pressure sterilizations, sterilize 30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811306719.2A CN109328875A (en) | 2018-11-05 | 2018-11-05 | A kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811306719.2A CN109328875A (en) | 2018-11-05 | 2018-11-05 | A kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109328875A true CN109328875A (en) | 2019-02-15 |
Family
ID=65313633
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811306719.2A Pending CN109328875A (en) | 2018-11-05 | 2018-11-05 | A kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109328875A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115125148A (en) * | 2021-03-24 | 2022-09-30 | 宁夏泰胜生物科技有限公司 | Culture medium and culture method for producing cyclosporine by fermentation of Sinkiang saururi |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103330258A (en) * | 2013-07-17 | 2013-10-02 | 黄雄 | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof |
| CN104164369A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Method for cultivating cordyceps militaris liquid spawn |
| CN104757454A (en) * | 2015-03-20 | 2015-07-08 | 江南大学 | Chinese caterpillar fungus lotus root |
| CN107058134A (en) * | 2017-05-20 | 2017-08-18 | 天津市东方中滨农业科技有限公司 | A kind of breeding method of Cordyceps militaris strain and application and its nutrient solution used |
| CN107333568A (en) * | 2017-09-19 | 2017-11-10 | 张家港市藏联生物研究所有限公司 | A kind of more batches of culture techniques of Cordyceps militaris constant temperature |
| CN108373976A (en) * | 2016-10-13 | 2018-08-07 | 镇江市天益生物科技有限公司 | A kind of submerged fermentation prepares the production method of Cordyceps militaris spawn |
-
2018
- 2018-11-05 CN CN201811306719.2A patent/CN109328875A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103330258A (en) * | 2013-07-17 | 2013-10-02 | 黄雄 | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof |
| CN104164369A (en) * | 2014-08-06 | 2014-11-26 | 赵胜华 | Method for cultivating cordyceps militaris liquid spawn |
| CN104757454A (en) * | 2015-03-20 | 2015-07-08 | 江南大学 | Chinese caterpillar fungus lotus root |
| CN108373976A (en) * | 2016-10-13 | 2018-08-07 | 镇江市天益生物科技有限公司 | A kind of submerged fermentation prepares the production method of Cordyceps militaris spawn |
| CN107058134A (en) * | 2017-05-20 | 2017-08-18 | 天津市东方中滨农业科技有限公司 | A kind of breeding method of Cordyceps militaris strain and application and its nutrient solution used |
| CN107333568A (en) * | 2017-09-19 | 2017-11-10 | 张家港市藏联生物研究所有限公司 | A kind of more batches of culture techniques of Cordyceps militaris constant temperature |
Non-Patent Citations (5)
| Title |
|---|
| 周会明: "《食用菌栽培技术》", 31 May 2017, 中国农业大学出版社 * |
| 孙慧娟: "高寒地区蛹虫草摇瓶液体培养基优化", 《安徽农业科学》 * |
| 朱寿荣 等: "蛹虫草液体菌种培养和使用研究", 《安徽农业科学》 * |
| 马瑞霞 等: "《普通高等教育"十三五"规划教材 食用菌栽培学》", 31 August 2017, 中国轻工业出版社 * |
| 龙海涛 等: "蛹虫草摇瓶菌种培养基的优化", 《安徽农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115125148A (en) * | 2021-03-24 | 2022-09-30 | 宁夏泰胜生物科技有限公司 | Culture medium and culture method for producing cyclosporine by fermentation of Sinkiang saururi |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108504621B (en) | Culture medium for paecilomyces hepiali Cs-4 and preparation method thereof | |
| CN101703214A (en) | Lucid Ganoderma hypra powder or Lucid Ganoderma tea and double fermentation process | |
| CN108401794A (en) | A kind of armillaria mellea accreting with Rhizoma Gastrodiae liquid spawn production method and cultigen special culture media | |
| CN102154407A (en) | Corayceps militaris polysaccharide two-stage fermentation synthesis process | |
| CN107500847A (en) | A kind of Stropharia rugoso-annulata liquid spawn culture medium and preparation method thereof | |
| CN105733957A (en) | Method for culturing cordyceps militaris with slant solid strains effectively instead of liquid strains | |
| CN103820299A (en) | Worm grass mycelium fermented vinegar and preparation method thereof | |
| CN108676730A (en) | A kind of fermentation manufacturing technique of Paecilomyces hepiali chen Cs-4 | |
| CN102964170A (en) | Formula and preparation method of agaricus bisporus liquid spawn | |
| CN109429911A (en) | A kind of fermentation cordyceps sinensis culture solution and preparation method thereof prepared with jerusalem artichoke | |
| CN110184200B (en) | High-yield sparassis crispa mycelium fermentation medium and preparation method thereof | |
| CN102342218A (en) | Method for producing cordyceps sinensis hyphae by virtue of solid-state fermentation | |
| CN108004015A (en) | A kind of Suaeda salsa seed oil supercritical carbon dioxide extraction process | |
| CN107384824A (en) | Stalk rotten agent soon | |
| CN106434368A (en) | Culture method of ganoderma leucocontextum liquid strain | |
| CN106434820A (en) | Method for increasing content of intracellular ganoderma triterpene in ganoderma lucidum mycelia liquid fermentation | |
| CN109223835A (en) | A kind of method of cordyceps sinensis Relative Fungi common fermentation production Radix Astragali Radix Notoginseng mycoplasma | |
| CN109328875A (en) | A kind of Cordyceps militaris shaking flask bacterium culture medium and preparation method thereof containing lotus root starch | |
| CN105535035B (en) | A kind of Inonotus obliquus fermented and cultured composition and preparation method thereof | |
| CN108048329A (en) | A kind of method of microalgae cell broken wall | |
| CN103849575B (en) | A kind of production method of single cell protein | |
| CN109055284A (en) | A kind of the wine brewing strain of ocean acid-producing bacteria and its application | |
| CN108611381A (en) | It is a kind of to be conducive to the liquid fermentation culturing method that Flavonoid substances are enriched in Phellinus | |
| CN104649782A (en) | Agrocybe cylindracea liquid strain culture solution and culture method of agrocybe cylindracea liquid strain | |
| CN115039633A (en) | Artificial culture method for sporocarp of Isaria japonica |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190215 |