CN107500847A - A kind of Stropharia rugoso-annulata liquid spawn culture medium and preparation method thereof - Google Patents
A kind of Stropharia rugoso-annulata liquid spawn culture medium and preparation method thereof Download PDFInfo
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- CN107500847A CN107500847A CN201710986035.0A CN201710986035A CN107500847A CN 107500847 A CN107500847 A CN 107500847A CN 201710986035 A CN201710986035 A CN 201710986035A CN 107500847 A CN107500847 A CN 107500847A
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- culture medium
- corn flour
- stropharia rugoso
- potato
- sugar
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention provides a kind of Stropharia rugoso-annulata liquid spawn culture medium and preparation method thereof, raw material is potato and corn flour, and auxiliary material is sugar, potassium dihydrogen phosphate, magnesium sulfate and vitamin B1, the weight ratio of each component is potato：Corn flour：Sugar：Potassium dihydrogen phosphate：Magnesium sulfate：Vitamin B1=100 200:50‑75:10‑30:1‑3:0.5‑1.5：0.003‑0.01.Stropharia rugoso-annulata liquid spawn culture medium provided by the invention, the Spawn incubation time is greatly shortened, and the strain health cultivated, preparation method is simple, and raw material is easy to get, and cost is low.
Description
Technical field
The present invention relates to a kind of bacterium culture medium, more particularly to a kind of stropharia rugosoannulata strain culture medium；The invention further relates to
The preparation method of this stropharia rugosoannulata strain culture medium.
Background technology
Stropharia rugoso-annulata, also known as claret Stropharia rugoso-annulata, Stropharia rugoso annulata, Fei Shi Stropharia rugoso-annulatas etc., it is international mushroom class trade market
On one of ten big mushroom classes.Stropharia rugoso-annulata contains abundant protein, mineral matter, vitamin and polysaccharide, is the United Nations's grain farmer's group
Knit (FAO) and recommend one of mushroom kind of cultivation to developing country.Stropharia rugoso-annulata mushroom body is beautiful in colour, and leg slightly covers fertilizer, and meat cunning is tender,
It is nutritious, it is the edible mushroom rising star that China had just risen in recent years.Existing Stropharia rugoso-annulata culture medium is solid medium, mainly
It is made of using hardwood sawdust, its bacterium germination is slow, production of hybrid seeds cycle length, and easily pollution.
The content of the invention
It is an object of the invention to provide a kind of Stropharia rugoso-annulata liquid spawn culture medium, solves existing solid spawn production of hybrid seeds week
The problem of phase is long.
It is another object of the present invention to provide the preparation method of above-mentioned culture medium.
Technical scheme is as follows：A kind of Stropharia rugoso-annulata liquid spawn culture medium, raw material are potato and corn flour,
Auxiliary material is sugar, potassium dihydrogen phosphate, magnesium sulfate and vitamin B1, the weight ratio of each component is potato：Corn flour：Sugar：Di(2-ethylhexyl)phosphate
Hydrogen potassium：Magnesium sulfate：Vitamin B1=100-200:50-75:10-30:1-3:0.5-1.5：0.003-0.01.
Preferably, above-mentioned sugar is glucose or sucrose.
The preparation method of above-mentioned Stropharia rugoso-annulata liquid spawn culture medium, comprises the following steps：
Step 1, corn flour is weighed, add distilled water, the ratio of corn flour and distilled water is 50-75 grams of corn flour/800-
1500 milliliters of distilled water, boil 20-40 minutes, double gauze filtering, constant volume, obtain corn flour leachate.
Step 2, peeling potatoes, potato is weighed, stripping and slicing, adds step 1 gained corn flour filtrate, boils 20-40 points
Clock, double gauze filtering, constant volume, obtains corn potato leachate.
Step 3, corn potato leachate is heated to boiling point, adds auxiliary material stirring and dissolve.
Step 4, pH value is adjusted to 6.0-6.5；
Step 5, sterilized using autoclaving, sterilization pressure 100-110kPa, 20-40 points of sterilization time
Clock；After cooling, fluid nutrient medium is produced.
Preferably, the Adding Way of above-mentioned auxiliary material is to sequentially add potassium dihydrogen phosphate, magnesium sulfate, vitamin and sugar, is often added
Enter a kind of auxiliary material to be sufficiently stirred, dissolve it.
The present invention has the advantages that：
(1) Stropharia rugoso-annulata liquid spawn culture medium provided by the invention, using corn and potato as raw material, carbon is used as using sugar
The direct sources in source, PH is adjusted using potassium dihydrogen phosphate, there is provided P elements and potassium element, add magnesium sulfate to promote new old generation
Thank, add vitamin B1To promote mycelial growth, the Spawn incubation time is short, and growth ability is strong, is not easy to pollute.
(2) Stropharia rugoso-annulata liquid spawn culture medium preparation method provided by the invention, simple and easy, raw material is common, easily
Obtain, cost is low.
Embodiment
The embodiment of the present invention is described in further detail with reference to embodiment.
Embodiment
A kind of Stropharia rugoso-annulata liquid spawn culture medium, by 200 grams of potato, 50 grams of corn flour, 20 grams of glucose, di(2-ethylhexyl)phosphate
3 grams of hydrogen potassium, 1.5 grams of magnesium sulfate, vitamin B110 milligrams are made.
The preparation method of above-mentioned Stropharia rugoso-annulata liquid spawn culture medium, comprises the following steps：
Step 1,50 grams of corn flour is weighed, add 1000 milliliters of distilled water, boiled 30 minutes, double gauze filtering, is settled to
1000 milliliters, this is corn flour leachate；
Step 2, peeling potatoes, 200 grams are weighed, stripping and slicing, adds 1000 milliliters of corn flour filtrate, is boiled 30 minutes, it is double-deck
Filtered through gauze, 1000 milliliters are settled to, this is corn potato leachate；
Step 3,1000 milliliters of corn potato leachate is taken, be heated to boiling point, added 3 grams of potassium dihydrogen phosphate and be sufficiently stirred
Dissolve；Add 1.5 grams of magnesium sulfate and be sufficiently stirred and dissolve；Add vitamin B110 milligrams are sufficiently stirred and dissolve；Add glucose 20
Gram it is sufficiently stirred and dissolves；
Step 4, pH value is adjusted to be 6.5；
Step 5, sterilized using autoclaving, sterilization pressure 103.4kPa, sterilization time 30 minutes, cooling
Afterwards, fluid nutrient medium is produced.
With the bottled fluid nutrient medium 100ml of 250ml tapers, after sterilizing, it is statically placed in incubator 24 hours, determines pollution-free
Stropharia rugoso-annulata parent species are accessed afterwards.After inoculation, it is positioned in incubator, 24 hours is stood at 26 DEG C.Shaking table is transferred to be trained
Support, 26 DEG C, shaking speed 160r/min of temperature, culture can be prepared by liquid stropharia rugosoannulata strain in 5-6 days, and now nutrient solution is in saturating
Bright shape.Every milliliter of bacterium ball quantity 240-270, bacterium bulb diameter 1.00-1.15mm.
Stropharia rugoso-annulata liquid spawn culture medium provided by the invention, using corn and potato as raw material, carbon source is used as using sugar
Direct sources, using potassium dihydrogen phosphate adjust PH, there is provided P elements and potassium element, add magnesium sulfate to enhance metabolism,
Add vitamin B1To promote mycelial growth, the Spawn incubation time is greatly shortened, and growth ability is strong, is not easy
Pollution.
Stropharia rugoso-annulata liquid spawn culture medium preparation method provided by the invention is simple, and raw material is easy to get, and cost is low.
Claims (4)
- A kind of 1. Stropharia rugoso-annulata liquid spawn culture medium, it is characterised in that：Raw material is potato and corn flour, and auxiliary material is sugar, phosphorus Acid dihydride potassium, magnesium sulfate and vitamin B1, the weight ratio of each component is potato：Corn flour：Sugar：Potassium dihydrogen phosphate：Magnesium sulfate： Vitamin B1=100-200:50-75:10-30:1-3:0.5-1.5：0.003-0.01.
- 2. Stropharia rugoso-annulata liquid spawn culture medium as claimed in claim 1, it is characterised in that：The sugar is glucose or sugarcane Sugar.
- 3. the preparation method of Stropharia rugoso-annulata liquid spawn culture medium as claimed in claim 1, it is characterised in that：Including following step Suddenly：Step 1, corn flour is weighed, add distilled water, the ratio of corn flour and distilled water is 50-75 grams of corn flour/800-1500 millis Distilled water is risen, is boiled 20-40 minutes, double gauze filtering, constant volume, obtains corn flour leachate；Step 2, peeling potatoes, potato is weighed, stripping and slicing, step 1 gained corn flour filtrate is added, boils 20-40 minutes, it is double Layer filtered through gauze, constant volume, obtains corn potato leachate；Step 3, step 2 gained corn potato leachate is heated to boiling point, adds auxiliary material stirring and dissolve；Step 4, pH value is adjusted to 6.0-6.5；Step 5, sterilized using autoclaving, sterilization pressure 100-110kPa, sterilization time 20-40 minutes；It is cold But after, fluid nutrient medium is produced.
- 4. the preparation method of Stropharia rugoso-annulata liquid spawn culture medium as claimed in claim 3, it is characterised in that：In step 3, institute The Adding Way for stating auxiliary material is to sequentially add potassium dihydrogen phosphate, magnesium sulfate, vitamin and sugar, and it is i.e. abundant often to add a kind of auxiliary material Stirring, dissolves it.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710986035.0A CN107500847A (en) | 2017-10-20 | 2017-10-20 | A kind of Stropharia rugoso-annulata liquid spawn culture medium and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710986035.0A CN107500847A (en) | 2017-10-20 | 2017-10-20 | A kind of Stropharia rugoso-annulata liquid spawn culture medium and preparation method thereof |
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| CN107500847A true CN107500847A (en) | 2017-12-22 |
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| CN201710986035.0A Pending CN107500847A (en) | 2017-10-20 | 2017-10-20 | A kind of Stropharia rugoso-annulata liquid spawn culture medium and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901612A (en) * | 2018-06-22 | 2018-11-30 | 伊犁师范学院 | A kind of elm mushroom liquid spawn culture medium and preparation method thereof |
| CN109006181A (en) * | 2018-08-28 | 2018-12-18 | 铜陵盛牛菌业有限责任公司 | A kind of edible fungus liquid culture growth medium and preparation method thereof |
| CN110452823A (en) * | 2019-10-11 | 2019-11-15 | 鲁东大学 | A kind of fluid nutrient medium of stropharia rugosoannulata strain and preparation method thereof |
| CN110574633A (en) * | 2019-08-22 | 2019-12-17 | 江苏东越生物技术发展股份有限公司 | liquid culture medium for industrialized production of stropharia rugoso-annulata and preparation method thereof |
| CN114621876A (en) * | 2021-04-30 | 2022-06-14 | 宝丰县弘亚食用菌科技有限公司 | Preparation method of stropharia rugoso-annulata strain |
| CN116034813A (en) * | 2022-12-13 | 2023-05-02 | 新疆农业科学院植物保护研究所 | Stropharia rugoso-annulata generation medium and fungus bag preparation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104855142A (en) * | 2015-06-12 | 2015-08-26 | 河北大学 | Method for using cattail crumbs to cultivate stropharia rugoso-annulata |
| CN106699412A (en) * | 2017-02-10 | 2017-05-24 | 利金站 | Method for cultivating stropharia rugosoannulata farlow |
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2017
- 2017-10-20 CN CN201710986035.0A patent/CN107500847A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104855142A (en) * | 2015-06-12 | 2015-08-26 | 河北大学 | Method for using cattail crumbs to cultivate stropharia rugoso-annulata |
| CN106699412A (en) * | 2017-02-10 | 2017-05-24 | 利金站 | Method for cultivating stropharia rugosoannulata farlow |
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| 孟庆国: "大球盖菇", 《食用菌》 * |
| 张琪林 等: "大球盖菇液体培养生育条件研究", 《农业与技术》 * |
| 王德芝 等: "《现代食用菌生产技术》", 31 August 2012, 华中科技大学出版社 * |
| 聂建军 等: "酒红球盖菇液体摇瓶培养基优化", 《山西农业科学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901612A (en) * | 2018-06-22 | 2018-11-30 | 伊犁师范学院 | A kind of elm mushroom liquid spawn culture medium and preparation method thereof |
| CN109006181A (en) * | 2018-08-28 | 2018-12-18 | 铜陵盛牛菌业有限责任公司 | A kind of edible fungus liquid culture growth medium and preparation method thereof |
| CN110574633A (en) * | 2019-08-22 | 2019-12-17 | 江苏东越生物技术发展股份有限公司 | liquid culture medium for industrialized production of stropharia rugoso-annulata and preparation method thereof |
| CN110452823A (en) * | 2019-10-11 | 2019-11-15 | 鲁东大学 | A kind of fluid nutrient medium of stropharia rugosoannulata strain and preparation method thereof |
| CN114621876A (en) * | 2021-04-30 | 2022-06-14 | 宝丰县弘亚食用菌科技有限公司 | Preparation method of stropharia rugoso-annulata strain |
| CN116034813A (en) * | 2022-12-13 | 2023-05-02 | 新疆农业科学院植物保护研究所 | Stropharia rugoso-annulata generation medium and fungus bag preparation method |
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