CN103864477A - Liquid strain medium of pure natural domestic fungus, manufacturing method and method for culturing strain of pure natural domestic fungus - Google Patents
Liquid strain medium of pure natural domestic fungus, manufacturing method and method for culturing strain of pure natural domestic fungus Download PDFInfo
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- CN103864477A CN103864477A CN201210524895.XA CN201210524895A CN103864477A CN 103864477 A CN103864477 A CN 103864477A CN 201210524895 A CN201210524895 A CN 201210524895A CN 103864477 A CN103864477 A CN 103864477A
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- juice
- bacteria liquid
- natural edible
- culture medium
- domestic fungus
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Abstract
The invention discloses a liquid strain medium of pure natural domestic fungus, a manufacturing method and a method for culturing the liquid strain of pure natural domestic fungus, and relates to the culture of liquid strain of the pure natural domestic fungus. The medium comprises the following raw materials: potato juice, honey, broad bean soup, Ficus microcarpa root tip juice, tomato juice, carrot juice and beef soup. Compared with the prior art, the liquid strain cultivated by the liquid strain medium of the pure natural domestic fungus has the advantages of normal growth, accelerated speed, reduction of long culture time and high pollution rate, and the domestic fungus liquid strain enables direct drinking.
Description
Technical field
The present invention relates to a kind of biotechnology and field of biological product, particularly a kind of natural edible bacteria liquid bacterium culture medium, making method and natural edible bacteria liquid method of breeding strain.
Background technology
Traditional edible fungus liquid culture growth medium is all the growth hormone as growth with chemical reagent, still, adopts the growth hormone of chemical reagent as growth, on the one hand poor growth, incubation time are long, pollution rate is high, and mycelia amount is low on the other hand, and the rate of taking over a job is not high yet.
Summary of the invention
One of object of the present invention is just to provide a kind of natural edible bacteria liquid bacterium culture medium, the natural edible bacteria liquid growth pollution rate normal, that speed is accelerated, minimizing incubation time is long that this natural edible bacteria liquid bacterium culture medium is cultivated is high, and edible fungi liquid strain is capable of direct drinking.
Technical scheme of the present invention is: a kind of natural edible bacteria liquid bacterium culture medium, this natural edible bacteria liquid bacterium culture medium is made up of following raw material: potato juice, honey, broad bean soup, Foliole banyan tip of a root juice, Tomato juice, Radix Dauci Sativae juice, beef soup.
As preferably, potato juice 220-250ml, honey 16-20ml, broad bean soup 220-250ml, Foliole banyan tip of a root juice 20-25ml, the 180-200ml of Tomato juice, Radix Dauci Sativae juice 80-100ml, beef soup 50-60ml.Two of object of the present invention is to provide a kind of making method of natural edible bacteria liquid bacterium culture medium.
Technical scheme is: a kind of making method of natural edible bacteria liquid bacterium culture medium, the making method of this natural edible bacteria liquid bacterium culture medium adopts above-mentioned raw material.
As preferably, the making method of this natural edible bacteria liquid bacterium culture medium comprises the following steps: by described raw material blending; Be settled to 1000ml; PH value is adjusted to 8 left and right; Heated and boiled 30min; Autoclaving 30-60min; Cooling.
Three of object of the present invention is to provide a kind of natural edible bacteria liquid method of breeding strain.
Technical scheme is: a kind of natural edible bacteria liquid method of breeding strain, this edible fungi liquid strain method of cultivation adopts above-mentioned natural edible bacteria liquid bacterium culture medium.
As preferably, this natural edible bacteria liquid method of breeding strain, comprises the following steps: inoculation, and shaking culture 48-60 hour, treats the growth of bacterium ball evenly; With applicator liquid inoculation bacterium bag.
Compared with prior art, beneficial effect of the present invention:
Because edible fungi liquid strain desired protein is provided by beef soup; VITAMIN is provided by potato, broad bean, tomato, Radix Dauci Sativae; growth hormone is provided by the Foliole banyan tip of a root; honey provides carbon, nitrogen element and Some Hormones; therefore the natural edible bacteria liquid growth pollution rate normal, that speed is accelerated, minimizing incubation time is long that the natural edible bacteria liquid bacterium culture medium of the present invention's protection is cultivated is high, and edible fungi liquid strain is capable of direct drinking.
In the present invention, potato juice, broad bean soup, beef soup are for maintaining agent, and honey, Foliole banyan tip of a root juice, Tomato juice, Radix Dauci Sativae juice are conditioning agent.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Producing of embodiment 1 potato juice
By potato slices add water remove slag after infusion 15-18min assorted.
Producing of embodiment 2 broad bean soup
By 500g broad bean shell add water remove slag after 10L infusion 20-30min assorted.
Producing of embodiment 3 Foliole banyan tip of a root juice
By the tip of a root of 1g leaflet banyan add remove slag after 0.5L water infusion 25-30min assorted.
Embodiment 4 Tomato juice produce
3KG tomato is squeezed the juice and added 200 L water and obtain.
Producing of embodiment 5 Radix Dauci Sativae juices
By 1KG Radix Dauci Sativae section add water remove slag after 20L infusion 25-30min assorted.
Producing of embodiment 6 beef soup
By life beef 500g add water remove slag after 10L infusion 30-35min assorted
Producing of embodiment 7 natural edible bacteria liquid bacterium culture mediums
Get respectively potato juice 220-250ml, the honey 16-20ml, broad bean soup 220-250ml, Foliole banyan tip of a root juice 20-25ml, the 180-200ml of Tomato juice, Radix Dauci Sativae juice 80-100ml, beef soup 50-60ml and the honey 18-20ml(that in embodiment 1-6, produce commercially available), mix, be settled to 1000ml, pH value is adjusted to 8 left and right, heated and boiled 30min, autoclaving 30-60min, be cooled to 30 DEG C following stand-by.
The cultivation of embodiment 8 natural edible bacteria liquid bacterial classifications
Get the liquid fermentation tank that is inoculated in embodiment 7, shaking culture 48-60 hour, frequency is per minute 80-90 time, and amplitude is 7-9cm, and temperature is controlled at 24-26 DEG C, treats the growth of bacterium ball evenly.
Be communicated with fermentor tank with the applicator liquid after sterilizing and under gnotobasis, access proper amount of strains to bacterium bag.。
Result please see the following form one.
Comparative example's 1 edible fungi liquid strain is cultivated
Obtaining liq substratum first boils potato when thoroughly well cooked but not mushy and extracts supernatant liquor, then prepares 3% glucose, 1% Semen Maydis powder, 0.05% magnesium sulfate, 0.1% potassium primary phosphate, finally potato supernatant liquor and above-mentioned solution and water is mixed.Liquid nutrient medium is applicable to the cultivation of multiple eating bacterium bacterial classification.
Under aseptic condition, bacterial classification is rendered in substratum.After shaking flask shaking culture liquid nutrient medium prepares, pack capacity into and be in the Erlenmeyer flask of 500 milliliters, every bottled enter 100 milliliters, sterilizing 30 minutes under pressure, then drops into the slant strains of approximately 2 square centimeters, at 23 DEG C～25 DEG C, leaves standstill and cultivates 24 hours.In the time of mycelium germination, be placed on shaking culture on reciprocating shaking table, oscillation frequency is per minute 80～100 times again, and amplitude is 6～10 centimetres.The temperature of shaking table chamber is controlled at 24 DEG C～25 DEG C, generally cultivates about 5 days.
Under gnotobasis, be inoculated into bacterium bag.
Result please see the following form one
Table one
| Substratum | Mycelia amount | The rate of taking over a job | Growth cycle |
| Comparative example 1 | 40-50% | 60-70% | 120 days |
| Embodiment 8 | 90% | 100% | 60 days |
Claims (6)
1. a natural edible bacteria liquid bacterium culture medium, this natural edible bacteria liquid bacterium culture medium is made up of following raw material:
Potato juice, honey, broad bean soup, Foliole banyan tip of a root juice, Tomato juice, Radix Dauci Sativae juice, beef soup 1.
2. natural edible bacteria liquid bacterium culture medium according to claim 1, is characterized in that:
Described potato juice 220-250ml, honey 16-20ml, broad bean soup 220-250ml, Foliole banyan tip of a root juice 20-25ml, the 180-200ml of Tomato juice, Radix Dauci Sativae juice 80-100ml, beef soup 50-60ml.
3. a making method for natural edible bacteria liquid bacterium culture medium, is characterized in that: the making method of this natural edible bacteria liquid bacterium culture medium adopts claim 1 or raw material claimed in claim 2.
4. the making method of natural edible bacteria liquid bacterium culture medium according to claim 3, is characterized in that comprising the following steps:
By described raw material blending;
Be settled to 1000ml;
PH value is adjusted to 8 left and right;
Heated and boiled 30min;
Autoclaving 30-60min;
Cooling.
5. a natural edible bacteria liquid method of breeding strain, is characterized in that: this edible fungi liquid strain method of cultivation adopts the natural edible bacteria liquid bacterium culture medium described in claim 1-4.
6. natural edible bacteria liquid method of breeding strain according to claim 5, is characterized in that comprising the following steps:
Inoculation, shaking culture 48-60 hour, treats the growth of bacterium ball evenly;
With applicator liquid inoculation bacterium bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210524895.XA CN103864477A (en) | 2012-12-10 | 2012-12-10 | Liquid strain medium of pure natural domestic fungus, manufacturing method and method for culturing strain of pure natural domestic fungus |
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| CN201210524895.XA CN103864477A (en) | 2012-12-10 | 2012-12-10 | Liquid strain medium of pure natural domestic fungus, manufacturing method and method for culturing strain of pure natural domestic fungus |
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| CN103864477A true CN103864477A (en) | 2014-06-18 |
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| CN201210524895.XA Pending CN103864477A (en) | 2012-12-10 | 2012-12-10 | Liquid strain medium of pure natural domestic fungus, manufacturing method and method for culturing strain of pure natural domestic fungus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063170A (en) * | 2015-08-03 | 2015-11-18 | 中国热带农业科学院海口实验站 | Xylanase production microbiological screening culture medium and culture method thereof |
| CN115104481A (en) * | 2021-12-31 | 2022-09-27 | 林礼 | Method for liquid culture of Huang cluster bacterium strain |
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| CN101353618A (en) * | 2007-07-25 | 2009-01-28 | 上海市农业科学院 | Production method of edible fungus liquid spawn and fermentation plant thereof |
| CN101695257A (en) * | 2009-10-30 | 2010-04-21 | 上海芝草生物技术有限公司 | Solid fermentation method for cordyceps sinensis |
| CN102229509A (en) * | 2011-06-17 | 2011-11-02 | 兴文县石海珍稀农产品开发有限责任公司 | Liquid culture medium applicable to various edible fungi |
| CN102643753A (en) * | 2011-02-22 | 2012-08-22 | 长江大学 | Liquid culture medium for efficiently producing Piriformospora indica |
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2012
- 2012-12-10 CN CN201210524895.XA patent/CN103864477A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101353618A (en) * | 2007-07-25 | 2009-01-28 | 上海市农业科学院 | Production method of edible fungus liquid spawn and fermentation plant thereof |
| CN101695257A (en) * | 2009-10-30 | 2010-04-21 | 上海芝草生物技术有限公司 | Solid fermentation method for cordyceps sinensis |
| CN102643753A (en) * | 2011-02-22 | 2012-08-22 | 长江大学 | Liquid culture medium for efficiently producing Piriformospora indica |
| CN102229509A (en) * | 2011-06-17 | 2011-11-02 | 兴文县石海珍稀农产品开发有限责任公司 | Liquid culture medium applicable to various edible fungi |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063170A (en) * | 2015-08-03 | 2015-11-18 | 中国热带农业科学院海口实验站 | Xylanase production microbiological screening culture medium and culture method thereof |
| CN105063170B (en) * | 2015-08-03 | 2018-11-13 | 中国热带农业科学院海口实验站 | A kind of production zytase microorganism screens culture medium and its cultural method |
| CN115104481A (en) * | 2021-12-31 | 2022-09-27 | 林礼 | Method for liquid culture of Huang cluster bacterium strain |
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Application publication date: 20140618 |