CN102389024A - Method for producing feeding peanut peptide by anaerobic fermentation of peanut meal - Google Patents
Method for producing feeding peanut peptide by anaerobic fermentation of peanut meal Download PDFInfo
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- CN102389024A CN102389024A CN2011103564676A CN201110356467A CN102389024A CN 102389024 A CN102389024 A CN 102389024A CN 2011103564676 A CN2011103564676 A CN 2011103564676A CN 201110356467 A CN201110356467 A CN 201110356467A CN 102389024 A CN102389024 A CN 102389024A
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Abstract
The invention relates to a method for producing feeding peanut peptides by anaerobic fermentation of peanut meal, which comprises the following steps: respectively preparing bacterial liquid of lactobacillus fermentum, saccharomyces cerevisiae, and bacillus subtillis, mixing peanut meal, corn meal, and potassium dihydrogen phosphate according to a mass ratio of 90:9:0.9:0.1 to prepare solid materials, well mixing the solid materials and mixed bacterial liquid according to a mass ratio of 6:4, performing fermentation, maintaining the fermentation temperature at 37 +/- 2 DEG C for 72-80 hours, when the fermentation is completed, drying the fermented materials till the water content is 11-12%, crushing to 60-70 meshes to obtain the feeding peanut peptides. The invention degrades the protein in the peanut meal into small peptides or amino acids to a great extent, and biodegrades the aflatoxin in the peanut meal; a lot of protease is generated during the fermentation process, and a large amount of lactic acids and a lot of beneficial microorganisms are contained in the product; the fermentation product is a good raw material for feed, and has good application prospects.
Description
Technical field
The invention belongs to peanut meal and produce the field of feedstuff, particularly a kind of method of utilizing the peanut meal anaerobic fermentation to produce feeding peanut peptide.
Background technology
Peanut meal is that peanut meal divides the dregs of rice, the secondary dregs of rice one time usually from the product of shelled peanut after oil plant is refined in squeezing.The meaning of dregs of rice is that the secondary dregs of rice promptly squeezed twice peanut dregs through the remaining peanut dregs of first squeezing.Usually the output of peanut meal can reach more than 44%, and the oil yield that is to say peanut is more than 55%, so the output of peanut meal is fewer relatively.Peanut meal is rich in vegetable protein, and its mouthfeel is better, relatively is suitable for using in the fowl poultry aquatic feeds.
Because peanut meal very easily produces aflatoxin when depositing, make its quality as feedstuff greatly reduce.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method of utilizing the peanut meal anaerobic fermentation to produce feeding peanut peptide; This method; Make the protein in the peanut meal be degraded to little peptide or amino acid largely, make aflatoxin in the peanut meal again by biodegradation.
A kind of method of utilizing the peanut meal anaerobic fermentation to produce feedstuff of the present invention comprises:
(1) prepare the MRS fluid nutrient medium, 121～126 ℃ of sterilizations, anaerobic fermentation is carried out according to 3～4% aseptic kind of the lactobacillus fermentis of pressing of gross mass in the cooling back under 37 ± 2 ℃, and after fermentation in 48～54 hours finishes, and gets lactobacillus fermenti bacterium liquid;
(2) prepare malt juice liquid medium, 121～126 ℃ of sterilizations, aerobic fermentation is carried out according to 3～4% aseptic kind of the saccharomyces cerevisiaes of pressing of gross mass in the cooling back under 37 ± 2 ℃, and fermentation in 20～24 hours finishes, and gets lactobacillus fermenti bacterium liquid;
(3) prepare the LB fluid nutrient medium, 121～126 ℃ of sterilizations, a Bacillus subtillis is planted according to 3～4% aseptic pressing of gross mass in the cooling back, under 37 ± 2 ℃, carries out anaerobic fermentation, and after fermentation in 20～24 hours finishes, and gets Bacillus subtillis bacterium liquid; (commercially available)
(4) the bacterium liquid that above-mentioned steps (1)～(3) is made mixes by equal-volume, gets mixed bacteria liquid; Peanut meal, corn flour, dusty yeast and potassium dihydrogen phosphate were pressed mass ratio 90: 9: 0.9: 0.1 mixing manufacture solid material; Solid material and mixed bacteria liquid are mixed by mass ratio at 6: 4, ferment, keeping fermentation temperature is 37 ± 2 ℃; After 72～80 hours, fermentation finishes;
(5) fermentation materials being dried to moisture is 11～12%, is crushed to 60～70 orders, promptly gets feeding peanut peptide.
MRS culture medium prescription in the said step (1) is casein peptone 10.0 grams, beef leaching thing 10.0 grams, yeast extract 5.0 grams; Glucose 5.0 grams, sodium acetate 5.0 grams, citric acid diamines 2.0 grams; Tween 80 1.0 grams, dipotassium hydrogen phosphate 2.0 grams, epsom salt 0.2 gram; Seven water manganese sulfates, 0.05 gram, calcium carbonate 20.0 grams, 1.0 liters of distilled water.
Malt extract medium prescription in the said step (2) is 100mL agar 2g, pH6.4.
Three kinds of bacterial classifications are had an effect during fermentation, and when just having packed container into, Bacillus subtillis and saccharomycete can utilize the air in the container to carry out aerobic fermentation, and produce protease.When the air of container was exhausted, lactobacillus fermenti began growth, produces a large amount of lactic acid, and can produce gas this moment, and gas can discharge through check valve.
Beneficial effect
The present invention adopts Bacillus subtillis, lactobacillus fermenti and three kinds of bacterial strains of saccharomycete that peanut meal is fermented, and makes the protein in the peanut meal be degraded to little peptide or amino acid largely, makes aflatoxin in the peanut meal by biodegradation again; Produced many protease in the sweat, contained the lactic acid and a large amount of beneficial microbes of high level in the product, tunning is a kind of feedstuff preferably, has a good application prospect.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
One, the preparation of bacterium liquid
1, the lactobacillus fermenti bacterial strain is rule cultivation with subsequent use on the MRS culture medium flat plate.
Preparation MRS fluid nutrient medium 4.8 liters of (casein peptone 10.0 grams, beef leaching thing 10.0 grams, yeast extract 5.0 grams, glucose 5.0 grams in 5 liters of fermentation tanks; Sodium acetate 5.0 grams, citric acid diamines 2.0 grams, Tween 80 1.0 grams; Dipotassium hydrogen phosphate 2.0 grams, epsom salt 0.2 gram, seven water manganese sulfates, 0.05 gram; Calcium carbonate 20.0 grams, 1.0 liters of distilled water), 121 ℃ of sterilizations; The cooling back is carried out anaerobic fermentation by kind of a lactobacillus fermenti 35 ℃ of conditions according to 3% ratio is aseptic, once stirs in per 3 hours.After 48 hours, the preparation of liquid bacterium liquid finishes.
2, with saccharomyces cerevisiae on malt extract medium, rule cultivate subsequent use.
The preparation malt juice liquid medium is 3 liters in 5 liters of fermentation tanks, 121 ℃ of sterilizations, and the cooling back is carried out aerobic fermentation by kind of a saccharomyces cerevisiae 35 ℃ of conditions according to 3% ratio is aseptic, once stirs in per 3 hours, and fermentation in 20 hours finishes.
3, Bacillus subtillis is rule cultivation with subsequent use on the LB culture medium flat plate.
Preparation LB fluid nutrient medium is 3 liters in 5 liters of fermentation tanks, 121 ℃ of sterilizations, and the cooling back is carried out anaerobic fermentation by kind of a Bacillus subtillis 35 ℃ of conditions according to 3% ratio is aseptic, once stirs in per 3 hours, and after 20 hours, the preparation of liquid bacterium liquid finishes.
4, three kinds of bacterium liquid are mixed with subsequent use with volume.
Two, solid fermentation
With peanut meal, corn flour, dusty yeast and potassium dihydrogen phosphate by 90: 9: 0.9: 0.1 mixed is made solid material (this moment, the material total moisture content was 11.5%).On the solid-liquid mixer, solid material and bacterium liquid are mixed by mass ratio at 6: 4, solidliquid mixture is filled in the airtight container (1 cube) that a band forward gas cuts down fermented, keeping fermentation temperature is 35 ℃, and after 72 hours, fermentation finishes.
It is 12% that fermentation materials is dried to moisture, is crushed to 60 orders, promptly gets feeding peanut peptide.
Embodiment 2
One, the preparation of bacterium liquid
1, the lactobacillus fermenti bacterial strain is rule cultivation with subsequent use on the MRS culture medium flat plate.
Preparation MRS fluid nutrient medium 4.8 liters of (casein peptone 10.0 grams, beef leaching thing 10.0 grams, yeast extract 5.0 grams, glucose 5.0 grams in 5 liters of fermentation tanks; Sodium acetate 5.0 grams, citric acid diamines 2.0 grams, Tween 80 1.0 grams; Dipotassium hydrogen phosphate 2.0 grams, epsom salt 0.2 gram, seven water manganese sulfates, 0.05 gram; Calcium carbonate 20.0 grams, 1.0 liters of distilled water), 126 ℃ of sterilizations; The cooling back is carried out anaerobic fermentation by kind of a lactobacillus fermenti 35 ℃ of conditions according to 4% ratio is aseptic, once stirs in per 3 hours.After 54 hours, the preparation of liquid bacterium liquid finishes.
2, with saccharomyces cerevisiae on malt extract medium, rule cultivate subsequent use.
The preparation malt juice liquid medium is 3 liters in 5 liters of fermentation tanks, 126 ℃ of sterilizations, and the cooling back is carried out aerobic fermentation by kind of a saccharomyces cerevisiae 35 ℃ of conditions according to 4% ratio is aseptic, once stirs in per 3 hours, and fermentation in 24 hours finishes.
3, Bacillus subtillis is rule cultivation with subsequent use on the LB culture medium flat plate.
Preparation LB fluid nutrient medium is 3 liters in 5 liters of fermentation tanks, 126 ℃ of sterilizations, and the cooling back is carried out anaerobic fermentation by kind of a Bacillus subtillis 39 ℃ of conditions according to 4% ratio is aseptic, once stirs in per 3 hours.After 24 hours, the preparation of liquid bacterium liquid finishes.
4, three kinds of bacterium liquid are mixed with subsequent use with volume.
Two, solid fermentation
With peanut meal, corn flour, dusty yeast and potassium dihydrogen phosphate by 90: 9: 0.9: 0.1 mixed is made solid material (this moment, the material total moisture content was 11.5%).On the solid-liquid mixer, solid material and bacterium liquid are mixed by mass ratio at 7: 3, solidliquid mixture is filled in the airtight container (1 cube) that a band forward gas cuts down fermented, keeping fermentation temperature is 37 ℃, and after 80 hours, fermentation finishes.
It is 11% that fermentation materials is dried to moisture, is crushed to 70 orders, promptly gets feeding peanut peptide.
Claims (3)
1. method of utilizing the peanut meal anaerobic fermentation to produce feeding peanut peptide comprises:
(1) prepare the MRS fluid nutrient medium, 121～126 ℃ of sterilizations, anaerobic fermentation is carried out according to 3～4% aseptic kind of the lactobacillus fermentis of pressing of gross mass in the cooling back under 37 ± 2 ℃, and after fermentation in 48～54 hours finishes, and gets lactobacillus fermenti bacterium liquid;
(2) prepare malt juice liquid medium, 121～126 ℃ of sterilizations, aerobic fermentation is carried out according to 3～4% aseptic kind of the saccharomyces cerevisiaes of pressing of gross mass in the cooling back under 37 ± 2 ℃, and fermentation in 20～24 hours finishes, and gets lactobacillus fermenti bacterium liquid;
(3) prepare the LB fluid nutrient medium, 121～126 ℃ of sterilizations, a Bacillus subtillis is planted according to 3～4% aseptic pressing of gross mass in the cooling back, under 37 ± 2 ℃, carries out anaerobic fermentation, and after fermentation in 20～24 hours finishes, and gets Bacillus subtillis bacterium liquid;
(4) the bacterium liquid that above-mentioned steps (1)～(3) is made mixes by equal-volume, gets mixed bacteria liquid; Peanut meal, corn flour, dusty yeast and potassium dihydrogen phosphate were pressed mass ratio 90: 9: 0.9: 0.1 mixing manufacture solid material; Solid material and mixed bacteria liquid are mixed by mass ratio at 6: 4, ferment, keeping fermentation temperature is 37 ± 2 ℃; After 72～80 hours, fermentation finishes;
(5) fermentation materials being dried to moisture is 11～12%, is crushed to 60～70 orders, promptly gets feeding peanut peptide.
2. a kind of method of utilizing the peanut meal anaerobic fermentation to produce feeding peanut peptide according to claim 1 is characterized in that: the MRS culture medium prescription in the said step (1) is casein peptone 10.0 grams, beef leaching thing 10.0 grams, yeast extract 5.0 grams; Glucose 5.0 grams, sodium acetate 5.0 grams, citric acid diamines 2.0 grams; Tween 80 1.0 grams, dipotassium hydrogen phosphate 2.0 grams, epsom salt 0.2 gram; Seven water manganese sulfates, 0.05 gram, calcium carbonate 20.0 grams, 1.0 liters of distilled water.
3. a kind of method of utilizing the peanut meal anaerobic fermentation to produce feeding peanut peptide according to claim 1 is characterized in that: the malt extract medium prescription in the said step (2) is 100mL agar 2g, pH6.4.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053787A (en) * | 2013-02-05 | 2013-04-24 | 杨庆利 | Method for preparing peanut peptide by microbial solid fermentation of peanut meal |
| CN104957370A (en) * | 2015-07-12 | 2015-10-07 | 宿州市笑果饲料科技有限责任公司 | Compound protein feed for poultry |
| CN108813107A (en) * | 2018-08-13 | 2018-11-16 | 吉林大学 | Peanut shell powder biological fermentation feed and preparation method thereof |
| CN112119834A (en) * | 2020-09-16 | 2020-12-25 | 聊城大学 | High-activity ganoderma lucidum culture medium |
| CN115141870A (en) * | 2022-09-05 | 2022-10-04 | 烟台融科生物科技有限公司 | Preparation method of peanut protein peptide |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053787A (en) * | 2013-02-05 | 2013-04-24 | 杨庆利 | Method for preparing peanut peptide by microbial solid fermentation of peanut meal |
| CN104957370A (en) * | 2015-07-12 | 2015-10-07 | 宿州市笑果饲料科技有限责任公司 | Compound protein feed for poultry |
| CN108813107A (en) * | 2018-08-13 | 2018-11-16 | 吉林大学 | Peanut shell powder biological fermentation feed and preparation method thereof |
| CN108813107B (en) * | 2018-08-13 | 2022-05-03 | 吉林大学 | Peanut shell powder biological fermentation feed and preparation method thereof |
| CN112119834A (en) * | 2020-09-16 | 2020-12-25 | 聊城大学 | High-activity ganoderma lucidum culture medium |
| CN115141870A (en) * | 2022-09-05 | 2022-10-04 | 烟台融科生物科技有限公司 | Preparation method of peanut protein peptide |
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Application publication date: 20120328 |