CN101525632A - Microecological preparation and preparation method thereof - Google Patents
Microecological preparation and preparation method thereof Download PDFInfo
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- CN101525632A CN101525632A CN200810008006A CN200810008006A CN101525632A CN 101525632 A CN101525632 A CN 101525632A CN 200810008006 A CN200810008006 A CN 200810008006A CN 200810008006 A CN200810008006 A CN 200810008006A CN 101525632 A CN101525632 A CN 101525632A
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Abstract
The invention relates to the technical field of treatment and comprehensive utilization of animal slaughter industrial wastewater, finds new application of blood wastewater and provides an animal-used microecological preparation which is prepared by taking blood wastewater as a raw material and selecting safe microbe. The animal-used microecological preparation is used in the field of livestock breeding, thereby solving the problem of environment pollution by blood wastewater and changing the waste into valuable and achieving the aim of clean production.
Description
Technical field:
The present invention relates to the improvement and the comprehensive utilization technique field of animal slaughtering trade effluent, the pollution treatment that is specifically related to the waste water of blood course of processing generation is handled and comprehensive utilization.Specifically, be a kind of be raw material with the blood wastewater, through fermentation process, be prepared into animal and use the probiotics product; The invention still further relates to of the application of this probiotics in the animal cultivation field.
Background technology:
Raising along with people's living standard, the consumption of meat is improving day by day, the industrial scale of animal slaughtering industry is also in continuous expansion, and the animal blood amount of Chan Shenging also increases day by day thereupon, and the enterprise of competitive bidding processing blood is also increasing, in the blood course of processing, can produce a large amount of waste water, this waste water mainly comes from equipment, processing place flushing etc., wherein contains hemocyte, plasma proteins, glycoprotein, antithrombotics etc., basic unharmful substance, COD reaches more than 10,000.The producer that has handles with the SBR method, because its processing cost is higher, effect is undesirable again, and at present, some waste water is discharged into rivers silently, the direct pouring farmland that has, because it is unprocessed, send the stench of death flavor after the blood ingredient corruption, cause serious environmental to pollute and extremely bad social influence, therefore, this waste water rational exploitation and utilization is extremely urgent.Up to the present, domestic achievement in research about this respect yet there are no report.
Summary of the invention:
The objective of the invention is to: hewing out the new purposes of blood wastewater, provide a kind of animal to use the probiotics product, is raw material with the blood wastewater promptly, selects for use safe microorganisms producing animal to use probiotics, and uses it for aquaculture; Thereby solve the pollution problem of blood wastewater, turn waste into wealth and realize the cleaner production target environment.
The objective of the invention is to realize by following technical scheme:
A kind of application of blood wastewater is characterized in that producing animal with blood wastewater uses probiotics.
A kind of animal is used probiotics, and said preparation is to be prepared from through microbial fermentation with blood wastewater.
The bacterial classification that above-mentioned blood wastewater microbial fermentation is selected for use is yeast, milk-acid bacteria and genus bacillus; Preferably saccharomyces cerevisiae (Saccharomyces cerevisiae Mcycn cx Hanscn) wherein, lactobacillus (Lactobacillus), subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformis), bacillus cereus (Bacillus cereus) is as preferred bacterial classification.The bacterial classification that the present invention uses comes from China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC).
Method with microbe fermentation method processing blood waste water is: blood wastewater is driven in the fermentation unit, after not adding carbon source or supplementary carbon source routine disinfection, the composite bacteria that inserts yeast and milk-acid bacteria and genus bacillus ferments, and maturing fermentation liquid is animal and uses probiotics.
Concrete steps are as follows:
Strain preparation: the bacterial classification that the present invention uses is milk-acid bacteria and yeast and bacillus.
The preparation of seed liquor: substratum: carbon source 0.1-10%, nitrogenous source 0.1-3%, activation back yeast and milk-acid bacteria and genus bacillus are inserted in sterilization cooling back, controlled temperature 25-37 ℃, cultivated 24-72 hour, make yeast and milk-acid bacteria and genus bacillus compound seed liquid;
Fermentation: blood wastewater is squeezed in the fermentation unit, do not add or add the carbon source of 0.5-5%, inoculum size by fermentation volume 0.2-20% inserts compound seed liquid, stir, controlled temperature 25-37 ℃, intermittently aerated culture is 24-72 hour, detect pH value in the fermenting process, when pH value 4-3, fermentation stops, and maturing fermentation liquid is animal and uses probiotics.
Above-described carbon source mainly is selected from glucose, sucrose, maltose, lactose, molasses, brown sugar, dextrin, starch etc.; Described nitrogenous source is selected from peptone, yeast extract paste, yeast powder, extractum carnis, various protein powder, soya-bean cake branch, groundnut meal, ammonium salt, nitrate etc.
The present invention has following positively effect:
1, the present invention has hewed out the scientific and reasonable regeneration approach of blood wastewater, provides possibility for manufacturing enterprise realizes " cleaner production ", meets the strategy of sustainable development.
2, the present invention has developed new animal and has used probiotics, makes trade waste obtain regeneration, turns waste into wealth, thereby has solved its pollution problem to environment, and enterprise has not only saved the pollution treatment expense but also can create certain economic benefits;
3, animal provided by the invention can effectively prevent the intestinal tract disease of animal with probiotics, improves immunizing power, reduces hazard residue, produces nuisanceless animal product.Improve the economic benefit of aquaculture, improve Enterprises'Competitiveness.
Embodiment:
Embodiment:
Make embodiment 1: handle the double centner blood wastewater:
1, strain preparation: bacterial classification is selected yeast saccharomyces cerevisiae (Saccharomyes cerevisiae Meyen exHansen) CGMCC AS 2.121 for use, Lactobacterium acidophilum (L.acidophilus) CGMCC 1.1854, bacillus cereus (Bacillus cereus) CGMCC AS 1.895.
The preparation of seed liquor: substratum is: brown sugar 75 grams, peptone 15 grams, be settled to 3000 milliliters with blood wastewater, and sterilized 20 minutes for 115 ℃; Activated above-mentioned bacterial classification is inserted in the cooling back, and controlled temperature was cultivated 36 hours for 34 ± 1 ℃, made compound seed liquid;
2, fermentation: double centner fresh blood waste water is dropped in the fermentation unit, and sterilization inserts 3 liters of compound seed liquid and stirs, 34 ± 1 ℃ of controlled temperature, and in culturing process, intermittently aeration-agitation was cultivated 48 hours, detected PH3.6, and fermentation stops; Be animal after the check packing and use probiotics.
Make embodiment 2: handle the double centner blood wastewater:
1, strain preparation: bacterial classification is selected yeast saccharomyces cerevisiae (Saccharomyes cerevisiae Meyen exHansen) CGMCC AS 2.610 for use, lactobacillus delbruockii subspecies bulgaricus (L.adlbrueckii subspbulgaricus) CGMCC AS 1.1482, subtilis (Bacillus subtilis) CGMCCAS 1.140;
The preparation of seed liquor: substratum is: glucose 100 grams, yeast extract paste 100 restrain, are settled to 10 liters with blood wastewater, sterilize 15 minutes for 121 ℃; Above-mentioned each bacterial classification that activated is inserted in the cooling back, and controlled temperature was cultivated 60 hours for 28 ± 1 ℃, has both got yeast, milk-acid bacteria secondary compound seed liquid;
2, fermentation: the double centner blood wastewater is dropped in the fermentation unit, add 2 liters in molasses, sterilization inserts 10 liters of compound seed liquid, stirs, and controlled temperature is cultivated for 28 ± 1 ℃ and detected PH3.4 in 60 hours, and fermentation stops.Be animal after the check packing and use probiotics.
Make embodiment 3: handle the double centner blood wastewater:
1, strain preparation: bacterial classification is selected yeast saccharomyces cerevisiae (Saccharomyes cerevisiae Meyen exHansen) CGMCC AS 2.121 for use, lactobacillus fermentum (L.fermentum) AS 1.122, Bacillus licheniformis (Bacillus licheniformis) CGMCC AS 1.932.
The preparation of seed: substratum is: molasses 10 grams, extractum carnis 6 grams, be settled to 200 milliliters with blood wastewater, and sterilized 15 minutes for 121 ℃; The above-mentioned bacterial classification of activatory is inserted in the cooling back, and controlled temperature was cultivated 72 hours for 36 ± 1 ℃, got compound seed liquid;
2, fermentation: the double centner blood wastewater is dropped in the fermentation unit, add 1 kilogram of glucose, sterilization inserts above-mentioned seed liquor, stir, 36 ± 1 ℃ of cultivations of controlled temperature are in culturing process, intermittently aeration-agitation is cultivated and was detected PH3.5 in 72 hours, and fermentation stops.Be animal after the check packing and use the probiotics product.
Make embodiment 4: handle the double centner blood wastewater:
1, strain preparation: bacterial classification is selected yeast saccharomyces cerevisiae (Saccharomyes cerevisiae Meyen exHansen) CGMCC AS 2.121 for use, Lactobacterium acidophilum (L.acidophilus) CGMCC 1.1854, subtilis (Bacillus subtilis) CGMCC AS 1.140;
The preparation of seed liquor: substratum is: brown sugar 600 grams, peptone 100 grams, be settled to 20 liters with blood wastewater, and sterilized 20 minutes for 115 ℃; Activated above-mentioned bacterial classification is inserted in the cooling back, and controlled temperature was cultivated 36 hours for 32 ± 1 ℃, made compound seed liquid;
2, fermentation: double centner fresh blood waste water is dropped in the fermentation unit, add 5 kilograms of starch, sterilization inserts 20 liters of compound seed liquid and stirs, 32 ± 1 ℃ of controlled temperature in culturing process, intermittently stir, cultivated 72 hours, and detected PH3.6, fermentation stops; Be animal after the packing check and use probiotics.
Application Example 1: animal is used the application of probiotics in growing-finishing pig drinking-water:
Feed formulation: Semen Maydis powder 62%, wheat bran 20%, soya-bean cake 4%, extracting filter residue 12%, Methionin 0.24%, bone meal 0.79%, oyster shell whiting 0.74%, salt 0.23%.
Trophic level: crude protein 15.19%, calcium 0.6%, phosphorus 0.5%, salt 0.23%, Methionin 0.75%, methionine(Met)+Gelucystine 0.58%, digestible energy 13.84 million Jiao/kilograms.
Control group drinking public water supply, experimental group are drunk the 1% probiotics aqueous solution.
Test with pig is: Du Luoke * army herds cross-fertilize seed No. 1,20.4 kilograms of average starting weights, each 20 of experimental group and control groups, random packet, similarity condition was raised 100 days, and the average end of experimental group weighs 98.5 kilograms/head, feedstuff-meat ratio 3.57: 1, the average end of control group weighs 96.9 kilograms/head, feedstuff-meat ratio 3.69: 1.
Application Example 2: the application of probiotics in fryer drinking-water:
Feed formulation: Semen Maydis powder 60%, dregs of beans 28%, tropina 6%, grease 1%, Methionin 0.13%, methionine(Met) 0.14%, VITAMIN 0.01%, trace element 0.1%, tricalcium phosphate 1.5%, salt 0.3%, stone flour 0.82%.
Trophic level: crude protein 20.15%, Methionin 1.09%, methionine(Met) 0.45%, calcium 0.70%, phosphorus 0.67%, metabolizable energy 12.16 million Jiao/kilograms.
Control group drinking public water supply, experimental group are drunk the 1% probiotics aqueous solution.
Experiment is 2 ages in week of Ai Wei mattress with fryer, derives from the school graduates of Heilongjiang Province chicken house, and test group and control group respectively are 200, random packet, and the raising condition is identical, and deliver for sale 7 ages in week.
The result is: the average end of control group weighs 2.41 kilograms/, feedstuff-meat ratio 2.12: 1; The average end of test group weighs 2.48 kilograms/, 2.08: 1.
Claims (7)
1, a kind of application of blood wastewater is characterized in that: produce animal with blood wastewater and use probiotics.
2, a kind of animal is used probiotics, it is characterized in that: said preparation is to be that raw material is prepared from through microbial fermentation with the blood wastewater.
3, probiotics according to claim 2 is characterized in that: said preparation is to be that raw material selects for use yeast and milk-acid bacteria and genus bacillus complex ferment to be prepared from the blood wastewater.
4, according to claim 2 or 3 s' the probiotics of stating, it is characterized in that: said preparation is to be raw material with the blood wastewater, select for use yeast saccharomyces cerevisiae (Saccharomyces cerevisiaeMcycn cx Hanscn) and lactobacillus genus (Lactobacillus) belong in one or two kind of bacterium and subtilis (Bacillus subtilis) or bacillus cereus (Bacillus cereus) or Bacillus licheniformis (Bacillus licheniformis) complex ferment be prepared from.
5, prepare the method for claim 2 or 3 or 4 described probioticses, its step comprises:
The preparation of seed liquor: substratum: carbon source 0.1-10%, nitrogenous source 0.1-3%, activatory yeast and milk-acid bacteria and genus bacillus are inserted in sterilization cooling back, controlled temperature 25-37 ℃, intermittently aerated culture is 24-72 hour, makes yeast and milk-acid bacteria and genus bacillus compound seed liquid;
Fermentation: blood wastewater is driven in the fermentation unit, the carbon source that does not add carbon source or additional 0.5-5%, after the routine sterilization, insert yeast and milk-acid bacteria and the genus bacillus compound seed liquid of fermentation volume 0.2-20%, carry out fermentation culture in 25-38 ℃, maturing fermentation liquid is animal and uses probiotics.
6, method according to claim 5 is characterized in that: described carbon source mainly is selected from glucose, sucrose, maltose, lactose, molasses, brown sugar, dextrin, starch; Described nitrogenous source is selected from peptone, yeast extract paste, yeast powder, extractum carnis, protein powder, soybean cake powder, groundnut meal, ammonium salt, nitrate.
7, claim 1 or 2 or 3 or 4 or 5 s' states the application of probiotics in animal cultivation.
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| CN200810008006A CN101525632A (en) | 2008-03-03 | 2008-03-03 | Microecological preparation and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389024A (en) * | 2011-11-11 | 2012-03-28 | 江苏省海洋水产研究所 | Method for producing feeding peanut peptide by anaerobic fermentation of peanut meal |
| CN102499321A (en) * | 2011-10-26 | 2012-06-20 | 北京大北农科技集团股份有限公司 | Nonreactive fermented feed of mixed bacteria and preparation method thereof |
| CN108165513A (en) * | 2018-02-06 | 2018-06-15 | 南京农业大学 | A kind of composite bacteria preparation and prepare the method for compound bacteria-fermented feed using it |
-
2008
- 2008-03-03 CN CN200810008006A patent/CN101525632A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499321A (en) * | 2011-10-26 | 2012-06-20 | 北京大北农科技集团股份有限公司 | Nonreactive fermented feed of mixed bacteria and preparation method thereof |
| CN102499321B (en) * | 2011-10-26 | 2014-05-21 | 北京大北农科技集团股份有限公司 | Nonreactive fermented feed of mixed bacteria and preparation method thereof |
| CN102389024A (en) * | 2011-11-11 | 2012-03-28 | 江苏省海洋水产研究所 | Method for producing feeding peanut peptide by anaerobic fermentation of peanut meal |
| CN108165513A (en) * | 2018-02-06 | 2018-06-15 | 南京农业大学 | A kind of composite bacteria preparation and prepare the method for compound bacteria-fermented feed using it |
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Open date: 20090909 |