CN101712927A - Preparation method for culturing ecological convenient bacteria - Google Patents
Preparation method for culturing ecological convenient bacteria Download PDFInfo
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- CN101712927A CN101712927A CN200910130970A CN200910130970A CN101712927A CN 101712927 A CN101712927 A CN 101712927A CN 200910130970 A CN200910130970 A CN 200910130970A CN 200910130970 A CN200910130970 A CN 200910130970A CN 101712927 A CN101712927 A CN 101712927A
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Abstract
The invention relates to a convenient preparation method for culturing ecological bacteria. The product comprises a composite microbial freeze-drying strain, a special culture medium, a flora coordination and growth promotion factor and the like. After the freeze-drying microbial strains and nutrients are mixed according to the weight ratio of 1:100-1:100000, finished products are obtained by sealing and encapsulating. When in use, the product is added with water with the quantity of 20 times, the mixture is stirred evenly, the environmental temperature is controlled between 15 DEG C and 40 DEG C, and the mixture is covered by a cover, sealed at a dry position and then can be fed after being naturally activated for 2-3 days. The added water is required to be drinking water and does not contain a disinfector, a bactericide, an antibiotic and a synthetic antibacterial agent, and the temperature of the water can not exceed 45 DEG C, warm water with the temperature of 25-37 DEG C is preferred; a used container must be washed and cleaned, is provided with a cover for sealing and can be recycled by disinfection after being used. At present, most of the active bacterium products sold in the market are packaged and delivered to the market after a microbial agent and the products are fermented in a factory, which is a sale-after-growth mode; the defects of unstable effect, unreasonable performance-price ratio and the like generally exist in the active bacterium products produced by this mode. The invention adopts a growth-after-sale mode and hands the production priority of a microbial preparation to a user, and the user can prepare and culture the required ecological bacteria under a simple condition by carrying out water addition, temperature control and natural fermentation on the product. The convenient bacteria do not contain chemical medicaments and toxin and are ecological and environment-friendly.
Description
Technical field
The present invention relates to the microbial preparation preparation field, particularly relate to a kind of ecology of microorganism and preparation method easily of culturing.
Technical background
In recent years, microorganism and formulation products thereof have obtained application more and more widely in fields such as plantation, breed, environmental protection, medical treatment, health cares, and its preparation method is varied especially.At present, on the market finding viable bacteria product be mostly in factory with after the fermentation of microbial inoculum and product, packing is distributed on the market again, is the pattern of " selling after long ", the defective that the viable bacteria product ubiquity effect instability of this mode of manufacture, cost performance are unreasonable etc.Most viable bacteria products are in the batch production production process, must lean on specialized retort, reactor made, technical requirements is all higher with economic input, and the partial supplementary material of adding in the preparation process and additive easily produce chemical toxicity and residual, after special typing and packing, transportation and sale is in user's hand again, link multichain bar is long, the product activity is greatly reduced, be prone to the result of use wild effect, and increased cost greatly, make that most microbial preparation product prices are higher, domestic consumer is " with not rising " usually, " not handy " influenced the benign development of microorganism industry.
Summary of the invention
For the viable bacteria product that takes on a new look " is sold " the preparation process complexity that pattern exists after long, the technical requirements height, economy has high input, the all swivel links of product are long, the result of use instability, easily produce chemical toxicity, all deficiencies of the unreasonable grade of cost performance, the invention provides a kind of preparation method that makes things convenient for who cultures microbial inoculum, take the pattern of " it is long to sell the back ", give the user with the production initiative of viable bacteria product, the user can add the water control temperature and prepare voluntarily under physical environment and simple utensil condition, naturally activation can be used after 2～3 days, and do not contain any chemicals and toxic substance, convenient and swift, produce in situ, in-field use is active high, satisfactory for result.
The technical scheme that the present invention solves microbial preparation product problem is: product of the present invention comprises complex microorganism bacterial classification, special culture medium, flora coordination and urgees the long factor etc.The present invention adopts complex microorganism bacterial classification freeze drying technology, with the microbial strains of preferably combination preparation back freeze-drying, has save when domestic consumer oneself prepares a large amount of inputs to retort and technology; The present invention only needs to add water with nutritive substance under simple and easy condition and mixes, and temperature control 15-40 ℃, microorganism can breed voluntarily, and growth and breeding speed is fast, can be prepared into active bacterium liquid in 2～3 days, and the user prepares on the spot, and in-field use is active high, with low cost, convenient and swift; The selected nutritive substance of the present invention is food grade foodstuff additive or feed grade foodstuff additive, no chemical agent and toxin, ecological, environmental protective; Added selective depressant in the nutrition of the present invention, can guarantee that bacterial classification avoiding extraneous living contaminants in the fermenting process, the breeding success rate height voluntarily; The present invention has saved the process that traditional microniological proudcts batch production is produced, and has realized zero packing, zero transportation, nullring joint, and easy and simple to handle, the microbe colony coordinative role is obvious, good feeding effect.
The invention has the beneficial effects as follows: the invention provides the preparation method that makes things convenient for of a kind of breeding ecological bacterium, can allow user oneself oneself prepare on the spot and culture the desired microorganisms microbial inoculum, and active high, effective, ecological, environmental protective, cost is low, and is simple to operate.
Embodiment
The embodiment of product of the present invention is:
1, the screening of bacterial classification: choose excellent species, types of spawn comprises one or more in milk-acid bacteria, Lactobacterium acidophilum, plant lactobacillus, subtilis, streptococcus faecium, streptococcus faecalis, lactobacterium casei, pediococcus acidilactici, bifidus bacillus, bacillus natto, yeast, photosynthetic bacterium, Rhodopseudomonas palustris, the Bacillus licheniformis.
2, configuration substratum: according to the dissimilar different substratum of bacterial classification configuration composition, the major ingredient of substratum has glucose, brown sugar, lactose, inorganic salt, starch, extractum carnis, yeast powder, yeast extract paste, peptone, agar, water, citric acid, lactic acid, acetic acid, sodium hydroxide, potassium hydroxide, phosphate-buffered salt.Contain in the substratum and coordinate and the short long factor, major ingredient has VITAMIN, base, porphyrin and derivative thereof, sterol, amine, straight chain fatty acid, amino acid etc.
3, cultivation: can take the mode cultivation of liquid culture or solid culture.The cultivation condition is also different variant because of bacterial classification, and the cultivation temperature range is between 0-70 ℃ usually, and the cultivation time was at 1-3 days.
4, freeze-drying and packing: behind cultured bacterial classification (bacterial classification of liquid culture is wanted centrifugal anhydrating earlier) adding lyophilized vaccine, freeze-drying under-20～-40 ℃ freeze-drying environment.Lyophilized vaccine has glucose, trehalose, glycine, N.F,USP MANNITOL etc., the product after the freeze-drying, and the piece type is loose, and surface-area is big, is easy to packing.
5, use the present invention to prepare the embodiment of breeding ecological convenient bacteria:
During preparation, freeze-drying lactobacillus and nutritive substance were mixed by weight 1: 10～1: 100000, after adding 20 times of water yields and stirring evenly, envrionment temperature is controlled between 15-40 ℃, seals at the drying place, activation is after 2～3 days naturally, promptly be prepared into active bacterium liquid, can be feeding at any time.It is drinkable water that institute adds the water requirement, does not contain sterilizing agent, sterilant, microbiotic, synthesising bacteria anti-reflecting medicine, and water temperature can not surpass 45 ℃, preferably 25-37 ℃ warm water; Used container must clean up, and sealable lid is arranged, and can use repeatedly with the back sterilization.The bacterium liquid that activation is good can be preserved at dry shady and cool place.
6, the application example of product of the present invention: (following raw material is commercially available, and control group is a blank.)
Embodiment 1: commercially available freeze-drying photosynthetic bacterium bacterial classification 10g; Yeast extract paste 15g, sodium-acetate 17.5g, sodium-chlor 10g, sal epsom 5g, hydrogen sulfate dipotassium 5g, citric acid 10g, independent packing.During use it is mixed, add and under the sun, place about 7 days (about 35 ℃) behind the water 10000ml, can obtain every milliliter of microbial preparation that contains 2,000,000,000 left and right sides viable bacterias.This microbial inoculum has extraordinary effect to the healthy state that strengthens cultivated animals, particularly aquatic animal, promotes growth etc., and can improve water quality.
Product is at the effect of fryer field:
1, test group fryer (20 age in days) counterpoise 750 gram/only, control group counterpoise 670 grams/only;
2, the total food consumption 100kg of current period (20 days) experimental group (1100), control group (1100) is 80kg;
3, mortality ratio reduces by 5%; The ight soil stink reduces.
Embodiment 2: commercially available freeze-dried lactic acid bacteria kind 5g; Brown sugar 990g, yeast powder 10g, packing respectively.During use it is mixed, at room temperature place (about 20-25 ℃) about 2 days after adding water 9000ml, can obtain every milliliter of microbial preparation that contains hundred million left and right sides viable bacterias of 15-20.Said preparation has extraordinary effect to prevention cultivated animals diarrhoea, promotes growth etc.
Product is to piglet morbidity and dead influence of washing in a pan
Product to swine Fever Vaccine stress compensation effect
| Group | Piglet quantity | Weaned piglet on average weighed (kg) in 21 days |
| Do not inoculate swine Fever Vaccine+milk-acid bacteria group | ??218 | ??4.82 |
| The vaccination group | ??237 | ??4.16 |
| Vaccination+milk-acid bacteria group | ??321 | ??4.91 |
Embodiment 3: commercially available subtilis 10g; Peptone 300g, yeast powder 30g, commercially available tennecetin 5g, high-temperature sterilization, hybrid packed.Add 20 kilograms in water during use, at room temperature place about 2 days, and, can obtain every milliliter of microbial preparation that contains a viable bacteria about 3,000,000,000 with the small air pump inflation.Said preparation has extraordinary effect to improving breeding environment, fecal deodorizing etc.
Product is to the slaughterhouse wastewater treatment effect
| The pollutent title | ??COD | ??BOD | ??SS | Ammonia nitrogen |
| Unit | ??Mg/l | ??Mg/l | ??Mg/l | ??Mg/l |
| Content before using | ??3000 | ??98 | ??600 | ??1600 |
| Use back content | ??240 | ??97.4 | ??39.6 | ??218 |
| Remove rate (%) | ??92 | ??0.6 | ??93.4 | ??81.5 |
| Annual compliance rate (%) | ??100 | ??100 | ??100 | ??100 |
Product is to the distillery waste water treatment effect
| The pollutent title | ??COD | ??BOD | ??SS | Ammonia nitrogen |
| Unit | ??Mg/l | ??Mg/l | ??Mg/l | ??Mg/l |
| Content before using | ??3860 | ??490 | ??1890 | ??9 |
| Use back content | ??7.72 | ??1.17 | ??52.92 | ??0.79 |
| Remove rate (%) | ??99.998 | ??99.997 | ??99.97 | ??99.91 |
| Annual compliance rate (%) | ??100 | ??100 | ??100 | ??100 |
Product is to the municipal sewage treatment effect
| The pollutent title | ??COD | ??BOD | ??SS | Ammonia nitrogen |
| Unit | ??Mg/l | ??Mg/l | ??Mg/l | ??Mg/l |
| Content before using | ??590 | ??380 | ??150 | ??10 |
| Use back content | ??37.88 | ??22.91 | ??99.93 | ??30 |
| Remove rate (%) | ??93.58 | ??93.97 | ??10 | ??28 |
| Annual compliance rate (%) | ??100 | ??100 | ??100 | ??100 |
Embodiment 4: commercially available freeze-dried lactic acid bacteria 5g, live yeast 5g; Brown sugar 2500g, yeast powder 30g, lactic acid 25g mixes autoclave sterilization, respectively packing.During use,, at room temperature place (about 30-35 ℃) about 2 days after adding water 25000ml, can obtain every milliliter of microbial preparation that contains about hundred million viable bacterias of 20-30 its mixing.Said preparation promotes dietetic alimentation to improving the intestine of young pigs environment, promotes growth, reduces sickness rate or the like and has extraordinary effect.
Product is to the gaining effect () of piglet
| Divide thin | The nest number | Birth weight | 28 age in days individual weight kg | 35 age in days individual weight kg | Every net gain kg | Day weight gain contrast g/ day |
| Test group | ??20 | ??0.94±0.22 | ??5.73 | ??7.49±1.31 | ??6.55 | ??193 |
| Control group | ??20 | ??0.97±0.17 | ??5.21 | ??6.45±1.02 | ??5.48 | ??161 |
Product is to the gaining effect (two) of piglet
| Group | The nest number | A piglet number | Average nascent litter weight | Average weight of weaning litter | Increase weight than control group |
| Experimental group | ??60 | ??668 | ??16.1 | ??96.8 | ??+8.2 |
| Control group | ??12 | ??131 | ??16.3 | ??88.6 |
Product is to the integrated application effect of piglet
| Project | Test group | Control group | ??P |
| Nest number/the nest of participating in the experiment | ??15 | ??15 |
| Project | Test group | Control group | ??P |
| Piglet sum/the head of participating in the experiment | ??146 | ??138 | |
| Morbidity number/head | ??14 | ??48 | |
| Total incidence/% | ??9.59 | ??34.78 | |
| A nest number/head of all falling ill | ??0.93±0.80 | ??3.2±2.14 | ??0.00065 |
| The equal individual day weight gain/g of nest | ??213.8±12.31 | ??204±14.52 | ??0.05597 |
| The equal sickness rate of nest | ??9.94±8.18 | ??33.84±21.37 | ??0.00037 |
| A dead number | ??1 | ??2 |
Claims (13)
1. the preparation method of a breeding ecological convenient bacteria.Product of the present invention comprises microbial strains and the required nutrition of microorganism growth.
2. the described microbial strains of claim 1 is the freeze-dried microorganisms bacterial classification, comprises in milk-acid bacteria, Lactobacterium acidophilum, plant lactobacillus, subtilis, streptococcus faecium, streptococcus faecalis, lactobacterium casei, pediococcus acidilactici, bifidus bacillus, bacillus natto, yeast, photosynthetic bacterium, Rhodopseudomonas palustris, the Bacillus licheniformis one or more.
3. the described nutrition of claim 1 contains the required nitrogenous source of microorganism growth, comprises in peptone, yeast powder, yeast extract paste, the extractum carnis one or more.
4. the described nutrition of claim 3 contains the required carbon source of microorganism growth, comprises in brown sugar, glucose, starch, the lactose one or more.
5. the described nutrition of claim 3 contains the required mineral substance of microorganism growth, comprises in copper, iron, zinc, manganese, calcium, molybdenum, sodium, the sylvite one or more.
6. contain acid-base modifier in the described nutrition of claim 3, comprise in citric acid, lactic acid, acetic acid, sodium hydroxide, potassium hydroxide, the phosphate-buffered salt one or more.
7. contain selective fungistat in the described nutrition of claim 3, comprise the selective antibiotic agent.
8. the described breeding ecological convenient bacteria of claim 1, described freeze-dried microorganisms bacterial classification and nutraceutical weight ratio of mixture are 1: 10～1: 100000.
9. described microorganism freeze-drying lactobacillus separates packing or hybrid packed alone with nutrition.
10. the preparation method of the described breeding ecological convenient bacteria of claim 1 is the described arbitrary breeding ecological convenient bacteria of claim 1～9 is added water, temperature control, spontaneous fermentation and to make.
11. among the described preparation method of claim 10, the described water that adds stirs evenly for adding 20 times of water yields, it is drinkable water that institute adds the water requirement, does not contain sterilizing agent, sterilant, microbiotic, synthesising bacteria anti-reflecting medicine.
12. among the described preparation method of claim 10, described temperature control is that water temperature is adjusted in 15 ℃-40 ℃, water temperature can not surpass 45 ℃, preferably 25-37 ℃ warm water.
13. among the described preparation method of claim 10, described spontaneous fermentation be meant the institute join bacterium liquid under envrionment temperature 15-40 ℃ condition, seal, activate 2～3 days naturally in the drying place.
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| CN200910130970A CN101712927A (en) | 2009-04-21 | 2009-04-21 | Preparation method for culturing ecological convenient bacteria |
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