CN102356815A - Solid-state fermentation method for producing microecological feed with rice bran as main raw material - Google Patents

Abstract

The invention discloses a solid-state fermentation method for producing a microecological feed with rice bran as a main raw material, which belongs to the technical field of fine processing of agricultural and sideline products. The method comprises the following steps: (1) combiningly inoculating probiotics of ferment bacteria, bacillus subtilis, microzyme, lactobacillus acidophilus and the like together on a yeast powder-peptone-agar slant and allowing a seed culture medium to be generated at a certain temperature in a certain period of time; (2) carrying out stabilization treatment on fresh rice bran by using the technology of twin-screw extrusion and carrying out sterilization on the rice bran by using the technology of microwave drying and sterilization; (3) inoculating seed hypha into the sterilized rice bran and carrying out solid-state fermentation at a constant temperature; (4) drying and crushing the rice bran after fermentation is finished to obtain fermented rice bran powder; (5) mixing the fermented rice bran powder with a protein feed (corn, soybean meal and the like) in proportion to prepare the microecological feed. Mycoprotein produced in the fermentation process of the rice bran can be directly absorbed by animals. The prepared microecological feed can improve digestive intestinal tract of animals and enhance body immunity of the animals. The invention provides the industries of livestock breeding with a novel, highly efficient, harmless and pollution-free option.

Description

A kind ofly be that with the rice bran primary raw material solid state fermentation produces the method for micro-ecological feed

Technical field

The present invention relates to a kind of method of producing micro-ecological feed, relate in particular to a kind of method of producing micro-ecological feed with the rice bran for the primary raw material solid state fermentation.

Background technology

In recent years, the security incident of food emerged in an endless stream, and each incident has all stayed painful memory to people later.Feed safety is paid close attention to by increasing people as " source " of food security.

Fast development along with animal husbandry; Popular probability takes place and also increases sharply in disease, and the raiser is interests abuse feed addictive, antibiotic or even a violated medicine of guaranteeing oneself, causes the overdose of livestock products residual; Get into human body through food, cause human body medicine to poison and allergic reaction.After livestock and poultry are used certain antibiotic for a long time; Bacterium produce to adapt to this type antimicrobial or inhereditary material is undergone mutation and form drug resistance; When in a single day the disease of this bacterium initiation takes place in people and animals; This type antibiotic reduces greatly the curative effect effect of disease or is entirely ineffective, gives in prevention and the treatment and causes difficulty.Want fundamentally to solve this difficult problem, just must form and set about, improve the animal and bird intestines environment, reduce the incidence of disease from feed.

The rice bran micro-ecological feed is to be natural medium with the rice bran, through modern biotechnology, to beneficial microbe through cultivate, ferment, drying makes the high-quality feed that is beneficial to growth.Have nontoxic, have no side effect, noresidue, pollution-freely do not develop immunity to drugs, characteristics such as with low cost, be desirable antibiotic substitute.It can replenish the normal microorganism that lacks or lack in the animal intestinal on quantity or kind, adjust or keep microecological balance in the enteron aisle, improves the also immunity function of enhancing body, improves the anti-stress ability of body.Simultaneously because controllability; Can improve nitrogen in the feed, phosphorus utilization; Reduce the animal dung urinary output; Thereby nitrogen, the heavy full pollution that belongs to environment reduce the consumption of herding specific yield to animal and plant resource, water resource in the minimizing feces of livestock and poultry, are only the act of " the effecting a permanent cure " of eliminating the animal husbandry environmental pollution.

Summary of the invention

The objective of the invention is to overcome the deficiency of prior art, a kind of method of producing micro-ecological feed with the rice bran for the primary raw material solid state fermentation is provided.

The present invention realizes through following technical scheme: a kind ofly be that with the rice bran primary raw material solid state fermentation produces the method for micro-ecological feed, may further comprise the steps:

(1) by weight, ferment bacterium 1-10 part, bacillus subtilis 1-8 part, saccharomycete 1-6 part, Lactobacillus acidophilus 1-8 part combination inclined-plane are inoculated on dusty yeast-peptone-agar slant,, generate seed culture medium in 30-40 ℃ of constant temperature culture 2-4 days;

(2) adopting Twin-Screw Extrusion Technology, is 125-145 ℃ in temperature, and pressure 0.5-0.7kpa carries out stabilization processes to fresh rice bran under the time 10-20s condition, uses the microwave disinfection technology that rice bran is carried out sterilization processing;

(3) the seed mycelium inoculation in the sterilized rice bran, is carried out solid state fermentation at 25 ℃ of constant temperature, constant humidity incubator in (2), fermentation time is 5-7 days;

(4) rice bran of accomplishing after fermenting obtains rice bran yeast powder after drying, pulverizing;

(5) rice bran yeast powder and protein feed are carried out the mixed micro-ecological feed that gets by mass ratio 1:4-6, protein feed comprises a kind of or mixture of corn, dregs of beans.

Technique effect of the present invention is: the mycoprotein that rice bran produces during the fermentation can directly be absorbed by animal.Improve the digestion enteron aisle of animal, improve the body immunity of animal.For feed and livestock and poultry breeding industry provide efficient, a harmless free of contamination new selection.

The specific embodiment

Below in conjunction with embodiment the present invention is done further explain.

Embodiment 1: be main material production solid state fermentation micro-ecological feed with the rice bran

Calculate by weight; Get the fresh rice bran that 100Kg removes impurity, adding water to the rice bran moisture is 16%-18%, adopts Twin-Screw Extrusion Technology; It is 135 ℃ that extrusion temperature is set; Pressure 0.6kpa carries out stabilization processes to fresh rice bran under the time 15s condition, is prone to cause the rotten lipolytic enzyme of rice bran, the vigor of lipoxidase with passivation.Rice bran after stable is put into the microwave disinfection machine, and it is 80 ℃ that sterilization temperature is set, and sterilizing time is 5min, carries out microwave disinfection, when rice bran is sterilized, also further plays the effect of rice bran stabilization.

Embodiment 2:

Calculate by weight, get and be inoculated on the PDA medium slant after four kinds of probios of ferment bacterium 0.5g, bacillus subtilis 0.3g, saccharomycete 0.25 g, Lactobacillus acidophilus 0.4 g mix,, generate seed culture medium in 30 ℃ of constant temperature culture 3-5 days.Be inoculated in then in the culture dish, in 30 ℃, relative humidity is that 65%-75% lucifuge is cultivated; After 8 days, under aseptic condition, cut into pieces; Be inoculated in the blake bottle of the sterilized 2Kg of being equipped with rice bran (inoculum concentration is 5 a bottles/culture dish); Continue at and cultivate 10d under the similarity condition, insert rice bran as the work bacterial classification then, inoculum concentration is 15% (be the work bacterial classification: the continent 15:85 of the Qin (W:W) sterilizes).Rice bran adds water with 1:1 (W:W) ratio in advance, and stirs, and reinstalls in the high-temperature resistance plastice, in 121 ℃ of 2h that sterilize down.Inoculation back rice bran places constant temperature and humidity culturing room, and temperature is 30 ℃, and relative humidity is to cultivate under 70% the condition.Till mycelia is covered with whole bag.Culture period is about 20d.After cultivating end, ferment rice bran is taken out, place under 60 ℃, forced air drying becomes the ferment rice bran original.

Embodiment 3:

Calculate by weight, the rice bran yeast powder 1Kg that takes by weighing embodiment 2 preparation mixes with common protein feed (corn and dregs of beans etc.) with 5Kg, and adorning both must rice bran micro-ecological feed finished product after wrapping.

Claims (1)

1. one kind is that with the rice bran primary raw material solid state fermentation produces the method for micro-ecological feed, it is characterized in that may further comprise the steps:

(1) by weight, ferment bacterium 1-10 part, bacillus subtilis 1-8 part, saccharomycete 1-6 part, Lactobacillus acidophilus 1-8 part combination inclined-plane are inoculated on dusty yeast-peptone-agar slant,, generate seed culture medium in 30-40 ℃ of constant temperature culture 2-4 days;

(2) adopting Twin-Screw Extrusion Technology, is 125-145 ℃ in temperature, and pressure 0.5-0.7kpa carries out stabilization processes to fresh rice bran under the time 10-20s condition, uses the microwave disinfection technology that rice bran is carried out sterilization processing;

(3) the seed mycelium inoculation in the sterilized rice bran, is carried out solid state fermentation at 25 ℃ of constant temperature, constant humidity incubator in (2), fermentation time is 5-7 days;

(4) rice bran of accomplishing after fermenting obtains rice bran yeast powder after drying, pulverizing;

(5) rice bran yeast powder and protein feed are carried out the mixed micro-ecological feed that gets by mass ratio 1:4-6, protein feed comprises a kind of or mixture of corn, dregs of beans.