CN105941899A - Production method for compound micro-ecological preparation for weaned piglets - Google Patents
Production method for compound micro-ecological preparation for weaned piglets Download PDFInfo
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- CN105941899A CN105941899A CN201610269612.XA CN201610269612A CN105941899A CN 105941899 A CN105941899 A CN 105941899A CN 201610269612 A CN201610269612 A CN 201610269612A CN 105941899 A CN105941899 A CN 105941899A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention discloses a production method for producing a feed micro-ecological preparation instead of antibiotics through fermentation with a mixed microorganism of bacillus coagulans and lactobacillus plantarum, and belongs to the technical field of microbial fermentation. The method includes the steps and processes: (1) culture of a seed liquid of bacillus coagulans; (2) bacillus coagulans serobic solid fermentation; (3) culture of a seed liquid of lactobacillus plantarum; and (4) bacillus coagulans and lactobacillus plantarum mixed solid fermentation. The lactobacillus plantarum is produced while the bacillus coagulans is produced, the no-sterilization raw material mixed fermentation method is adopted, the production cost of the product is remarkably reduced, and the application of the product in livestock and poultry feeds, aquatic feeds and the like can be promoted.
Description
Technical field
The present invention relates to technical field of microbial fermentation, particularly relate to Bacillus coagulans and plant
A kind of mixed microorganism fermentation of thing lactobacillus, produces feeding micro-ecological preparation substitute antibiotics
The production method of thing.
Technical background
Antibiotic being widely used on poultry, enables plant to limit the cultured space of domestic animal
And activity, keep animal to be in optimum health state simultaneously, the application of antibiotic promotes herding
The development of industry, allows more people buy more meat products with cheap price.With regard to livestock and poultry breeding industry
For, the long-term or abuse of antibiotics that exceeds standard, especially antibiotic is carried as feed additive
The harm come highlights the most day by day.European Union, Japan and Korea S prohibited respectively at 2006 and 2008
Only using antibiotic in feedstuff, the U.S. has planned to forbid using in feedstuff antibiosis in 2017
Element.Though China the most clearly proposes to prohibit the use of the timetable of antibiotic, but has carried out a large amount of
The research of feeding antibiotic succedaneum is demonstrated with producing, feeding in Substitutes For Antibiotic in recent years
Microorganism formulation demonstrates that good growth momentum demonstrates good growth momentum.
Bacillus coagulans is a kind of lactic acid producing to form again the probiotic bacteria of spore, at aerobic and
All can grow under conditions of anaerobism.There is the characteristic such as strong stress resistance, hot, the easy storage of high temperature resistant height,
The dual function entrance intestinal that can pass through gastric acid and digestive enzyme is grown surely.And sprout rapidly at intestinal
Send out and be grown to serve as trophosome viable bacteria metabolism generation lactic acid, thus improve its intestinal microbial population balance,
Harmful microbe quantity and diarrhea rate in animal intestinal can be significantly reduced, can improve daily gain,
Feed conversion rate, therefore, Bacillus coagulans is with a wide range of applications in feed industry.
The lactic acid bacteria of this class of Lactobacillus plantarum has proven to have and improves animal intestinal health and carry
The effect of high immunity, its lactic acid produced and lactein can directly act on pathogenic bacteria.
Being considered as the beneficial microbe of preferable growth promotion substitute antibiotics, having wide development should
Use prospect.
Owing to the nutritional condition of Lactobacillus plantarum requires harshness, it is presently mainly with Carnis Bovis seu Bubali cream, egg
The materials such as white peptone, yeast extract, glucose are culture medium, then add some growth stimulants.This
A little its market price of material for doing culture medium are the most agricultural and sideline compared with wheat bran, bean cake, Semen Maydis powder etc.
Product expensive go out manyfold, produce Lactobacillus plantarum with these materials, its cost to cultivation industry
As higher for feed additive, hinder to a certain extent the production of Lactobacillus plantarum with
In the cultivation popularization of industry, application.
Summary of the invention
Present invention aim at, it is provided that a kind of agricultural byproducts of being easy to get with cheap, material are
Raw material, is aided with rational fermentation technology, while producing Bacillus coagulans, with relatively low one-tenth
The method of this mixture manufacturing Lactobacillus plantarum.The object of the invention is come real by the following technical programs
Existing.
The production method of a kind of ablactational baby pig compound micro-ecological preparation, the method presses following step
Suddenly carry out:
(1) Bacillus coagulans (Bacillus coagulans strain number ACCC
00403) (Bacillus coagulans (Bacillus coagulans) involved in the present invention
Strain number is 00403, draws from Chinese agriculture Microbiological Culture Collection administrative center (ACCC)
Enter) liquid seeds cultivation: Bacillus coagulans slant strains is accessed in triangular flask culture medium,
500ml triangular flask culture medium loading amount is 100ml, through 35～38 DEG C, and 180～200rpm shaking tables
Triangular flask liquid seeds is become after cultivating 14～18h, standby；The seed culture of described triangular flask
Base is (g/L): lactose 1, peptone 1, beef extract 0.5, sodium chloride 0.5, and tune pH is
7.0～7.5,121 DEG C of sterilizing 15min；
(2) Bacillus coagulans aerobic solids fermentation culture: solid fermentation culture medium is bean cake,
The water adding bean cake weight 0.6 times is formulated, is pressed by every bottle of triangular flask liquid seeds of step (1)
Every bottle of seed: the ratio inoculation of 20～30 kilograms of bean cake in terms of solid content weight, it is not necessary to go out
Bacterium, after loading koji tray is 5～7cm to fermentation materials thickness, under 35～38 DEG C of room temperature conditions
Fermentation culture 20～24h, standby；
(3) Lactobacillus plantarum (Lactobacillus plantarum strain number ACCC
11016) (Lactobacillus plantarum (Lactobacillus plantarum) involved in the present invention
Strain number be 11016, from Chinese agriculture Microbiological Culture Collection administrative center (ACCC)
Introduce) seed liquor cultivate: in 500ml triangular flask load 300ml liquid MRS culture medium,
Access Lactobacillus plantarum strain, after 35～38 DEG C are cultivated 12～14h, in 0～4 DEG C of preservation,
Standby；Described MRS culture medium is (g/L): glucose 2, peptone 1, beef extract 1,
Yeast extract 0.5, dipotassium hydrogen phosphate 0.2, dibasic ammonium citrate 0.2, sodium acetate 0.5, tween
0.1, magnesium sulfate 0.058, manganese sulfate 0.025, adjusting pH is 5.8～6.2,121 DEG C of sterilizings
15min；
(4) Bacillus coagulans and the fermentation of Lactobacillus plantarum hybrid solid: hybrid solid ferments
Culture medium is: defatted rice bran: step (2) Bacillus coagulans aerobic solids fermentation culture medium
(based on bean cake weight before fermentation): calcium carbonate by weight 70～90: 10～30: 4～10
Proportions forms, and by this fermentation medium 0.5～0.7 times of the weight of defatted rice bran
Add tap water, it is not necessary to sterilizing, by the Lactobacillus plantarum seed liquor of step (3), mix by this
Close 1%～3% inoculation of the weight of defatted rice bran in fermentation medium, mix thoroughly, directly proceed to mould
In pocket, tighten plastic bag, drive the air in bag as far as possible away, continue 35～38 DEG C of cultivations
Under the conditions of cultivate and stop fermentation after 30～36h；Under the conditions of not higher than 50 DEG C, fermented product is dried
After doing, being ground into 40 mesh, check, be packaged into product.
On the other hand, the present invention provides a kind of by answering that method described in claim 1 produces
Close microbial ecological agent in preparation ablactational baby pig feedstuff, carry out the purposes of substitute antibiotics.Preferably,
Wherein, described feedstuff consists of the following composition: Semen Maydis 60.8%, bean cake 15.0%, expanded
Semen sojae atricolor 10.0%, fish flour 5.0%, whey powder 6.0%, calcium hydrogen phosphate 1.2%, stone powder 0.6%,
Salt 0.3%, lysine 0.1%, premix material 1.0%, the method as claimed in claim 1 of 0.4
The compound micro-ecological preparation produced.
On the one hand, the present invention provides a kind of ablactational baby pig mixed feed, wherein, this feedstuff
Become to be grouped into by following: Semen Maydis 60.8%, bean cake 15.0%, puffing Semen sojae atricolor 10.0%, fish flour
5.0%, whey powder 6.0%, calcium hydrogen phosphate 1.2%, stone powder 0.6%, salt 0.3%, lysine
0.1%, premix material 1.0%, the composite microbial that the method as claimed in claim 1 of 0.4% produces
State preparation.
Beneficial effects of the present invention
1, Bacillus coagulans and Lactobacillus plantarum are first prepared as seed by present invention process respectively
After, then by they combined inoculations by a certain percentage, carry out hybrid solid fermentation.When condensing spore
When bacillus is fermented, a certain amount of amylase and protease can be produced, can be by the albumen in bean cake
Decompose with the starch in defatted rice bran, generate the small-molecule substance such as aminoacid and monosaccharide, this
Sample Lactobacillus plantarum is with regard to the protein that can utilize and the aminoacid of amylolysis and monosaccharide etc.
Small-molecule substance, the efficient growth breeding for Lactobacillus plantarum can continuously provide required battalion
Support material；Secondly, Bacillus coagulans growth and produce while forming spore protease,
The bioactive substances such as amylase, these materials can be with Bacillus coagulans spore, plant breast bar
After bacterium and metabolite thereof add feedstuff simultaneously as additive, the most more can promote the health of poultry
With strengthening intestinal, nutrient substance is digested and assimilated, thus also improve mixed fermentation of the present invention and produce
The application advantage of product.
2, the present invention is easy to get with bean cake, defatted rice bran etc., cheap agricultural byproducts instead of in the past
Such as the fermentation raw material of Carnis Bovis seu Bubali cream, peptone, yeast extract, glucose etc., condense spore producing
Produce Lactobacillus plantarum while bacillus, and use unsterilised raw material mixed fermentation method,
Significantly reduce the production cost of product, can promote this product in the feedstuff such as poultry, Aquatic product
Application.
Detailed description of the invention
By following example, the present invention is described in further detail, but its summary of the invention is also
Do not placed restrictions on by these contents.
The bacterial strain of Bacillus coagulans (Bacillus coagulans) involved in the present invention
Numbered 00403, Lactobacillus plantarum (Lactobacillus plantarum) strain number
Be 11016 all from Chinese agriculture Microbiological Culture Collection administrative center (ACCC) introduce.
The production method 1 of an embodiment 1:(ablactational baby pig compound micro-ecological preparation)
Concrete technology of the present invention is as follows:
(1) Bacillus coagulans (Bacillus coagulans strain number ACCC
00403) seed liquor is cultivated: Bacillus coagulans slant strains accessed in triangular flask culture medium,
500ml triangular flask culture medium loading amount is 100ml, through 35～38 DEG C, and 180～200rpm shaking tables
Triangular flask liquid seeds is become after cultivating 14～18h, standby；The seed culture of described triangular flask
Base is (g/L): lactose 1, peptone 1, beef extract 0.5, sodium chloride 0.5, and tune pH is
7.0～7.5,121 DEG C of sterilizing 15min；
(2) Bacillus coagulans aerobic solids fermentation culture: solid fermentation culture medium is bean cake,
The water adding bean cake weight 0.6 times is formulated, is pressed by every bottle of triangular flask liquid seeds of step (1)
Every bottle of seed: the ratio inoculation of 20 kilograms of bean cake in terms of solid content weight, it is not necessary to sterilizing,
After loading koji tray is 5～7cm to fermentation materials thickness, in 35～38 DEG C of room temperature condition bottom fermentations
Cultivation 20～24h, standby；
(3) Lactobacillus plantarum (Lactobacillus plantarum strain number ACCC
11016) (Lactobacillus plantarum (Lactobacillus plantarum) involved in the present invention
Strain number be 11016, from Chinese agriculture Microbiological Culture Collection administrative center (ACCC)
Introduce) seed liquor cultivate: in 500ml triangular flask load 300ml liquid MRS culture medium,
Access Lactobacillus plantarum strain, after 35～38 DEG C are cultivated 12～14h, in 0～4 DEG C of preservation,
Standby；Described MRS culture medium is (g/L): glucose 2, peptone 1, beef extract 1,
Yeast extract 0.5, dipotassium hydrogen phosphate 0.2, dibasic ammonium citrate 0.2, sodium acetate 0.5, tween
0.1, magnesium sulfate 0.058, manganese sulfate 0.025, adjusting pH is 5.8～6.2,121 DEG C of sterilizings
15min；
(4) Bacillus coagulans and the fermentation of Lactobacillus plantarum hybrid solid: hybrid solid ferments
Culture medium is: defatted rice bran: step (2) Bacillus coagulans aerobic solids fermentation culture medium
(before fermentation based on bean cake weight): calcium carbonate by weight 90: 10: 10 proportions and
Become, and by 0.5 times of addition tap water of the weight of defatted rice bran, nothing in this fermentation medium
Need sterilizing, by the Lactobacillus plantarum seed liquor of step (3), by this mixed fermentive culture medium
1% inoculation of the weight of defatted rice bran, mixes thoroughly, directly proceeds to, in plastic bag, tighten plastic bag,
Drive the air in bag away, after continuing to cultivate 30～36h under 35～38 DEG C of condition of culture as far as possible
Stop fermentation；After fermented product being dried, is ground into 40 mesh under the conditions of not higher than 50 DEG C, inspection
Test, be packaged into product.
The production method 2 of an embodiment 2:(ablactational baby pig compound micro-ecological preparation)
In this example, step (2) Bacillus coagulans aerobic solids fermentation culture: solid fermentation
Culture medium is bean cake, and the water proportions adding bean cake weight 0.6 forms, by step (1) every bottle
Triangular flask liquid seeds is by every bottle of seed: the ratio of 25 kilograms of bean cake in terms of solid content weight
Inoculation, after loading koji tray is 5～7cm to fermentation materials thickness, in 35～38 DEG C of room temperature conditions
Bottom fermentation cultivation 20～24h, standby；
Step (4) Bacillus coagulans and the fermentation of Lactobacillus plantarum hybrid solid: hybrid solid
Fermentation medium is: defatted rice bran: the fermentation training of step (2) Bacillus coagulans aerobic solids
Support thing (based on bean cake weight before fermentation): calcium carbonate by weight 80: 20: 7 ratio mixes,
And by 0.6 times of addition tap water of the weight of defatted rice bran in this fermentation medium, it is not necessary to go out
Bacterium, by the Lactobacillus plantarum seed liquor of step (3) by defatted rice bran in this fermentation medium
2% inoculation of weight, mixes thoroughly, directly proceeds to, in plastic bag, tighten plastic bag, drive away as far as possible
Air in Dai, stops fermentation after continuing to cultivate 30～36h under 35～38 DEG C of condition of culture；
After fermented product being dried, is ground into 40 mesh under the conditions of not higher than 50 DEG C, check, be packaged into
Product.
Remaining technique, step are with embodiment 1.
The production method 3 of an embodiment 3:(ablactational baby pig compound micro-ecological preparation)
In this example, step (2) Bacillus coagulans aerobic solids fermentation culture: solid fermentation
Culture medium is bean cake, and the water proportions adding bean cake weight 0.6 forms, by step (1) every bottle
Triangular flask liquid seeds is by every bottle of seed: the ratio of 30 kilograms of bean cake in terms of solid content weight
Inoculation, after loading koji tray is 5～7cm to fermentation materials thickness, in 35～38 DEG C of room temperature conditions
Bottom fermentation cultivation 20～24h, standby；
Step (4) Bacillus coagulans and the fermentation of Lactobacillus plantarum hybrid solid: hybrid solid
Fermentation medium is: defatted rice bran: the fermentation training of step (2) Bacillus coagulans aerobic solids
Support thing (based on bean cake weight before fermentation): calcium carbonate by weight 70: 30: 4 ratio mixes,
And by 0.7 times of addition water of the weight of defatted rice bran in this fermentation medium, it is not necessary to sterilizing, will
The Lactobacillus plantarum seed liquor of step (3) is by the weight of defatted rice bran in this fermentation medium
3% inoculation, mixes thoroughly, directly proceeds to, in plastic bag, tighten plastic bag, drive the sky in bag as far as possible away
Gas, stops fermentation after continuing to cultivate 30～36h under 35～38 DEG C of condition of culture；The highest
After fermented product being dried, is ground into 40 mesh under the conditions of 50 DEG C, check, be packaged into product.
Remaining technique, step are with embodiment 1.
Embodiment 4 (ablactational baby pig feeding effect contrast test)
The premix material used in this example: produced by Zhejiang Academy of Agricultural Science animal and veterinary institute
And provide.Select 35 age in days wean three way cross piglets 120, be divided into three process groups,
Often group 40:
Negative control group (without antibiotic), positive control group (antibiotic group) and test group is (no
Added with antibiotic, adds product of the present invention).Free choice feeding during test, freely drinks water.Calculate
The initial average weight of test, off-test average weight, average daily gain, average daily ingestion amount,
Feedstuff-meat ratio, every day, observed and recorded diarrhoea situation, calculated diarrhoea probability.
Negative control group Diet Formula is: Semen Maydis 60.8%, bean cake 15.0%, puffing Semen sojae atricolor 10.0%,
Fish flour (Peru) 5.0%, whey powder 6.0%, calcium hydrogen phosphate 1.2%, stone powder 0.6%, salt 0.3%,
Lysine 0.1%, premix material (some trace element, vitamin, aminoacid, inserts carrier
Mixture) 1.0% (not added with antibiotic).
Positive control group (antibiotic group) is that to add antibiotic on the basis of negative control group daily ration (anti-
Raw element is bambermycin and chlortetracycline) (the negative control daily ration of 400 grams/1000 kilograms is joined) (first and
Even batch mixing mixes, and is then mixing with other component).
On the basis of test group (adding product of the present invention) Diet Formula is negative control group Diet Formula
Adding the fermented product of 0.4% embodiment 1, respectively at adding, latter 30 days statistical survey piglets are raw
Long performance.The results are shown in Table 1.
Interpretation of result: product of the present invention, with compared with antibiotic group, is increasing weight and diarrhea rate
Aspect is significantly improved, and compared with using antibiotic group, preventing and treating diarrhea rate makes moderate progress,
Weightening finish aspect is close to the level of antibiotic.Product energy substitute antibiotics of the present invention is on ablactational baby pig
Application.
Table 1: the compound micro-ecological preparation impact (examples of implementation 1) on Production Performance of Weaning Pigs.
It addition, according to above method, be negative control to test group (adding product of the present invention) Diet Formula
The fermented product of 0.4% embodiment 2 or 3 is added, after adding 30 on the basis of group Diet Formula
It statistical survey piglet growth performance, result also indicates that, the average daily gain (kg/d) of experimental group
It is 523.14 ± 134.91 (embodiments 2) and 521.14 ± 134.91 (embodiments 3), diarrhoea point
It is not 3.1 and 3.5%.
Equally, when Bacillus coagulans fermentation or the Lactobacillus plantarum using the independent present invention
(i.e. in examples of implementation 1, the amount of Lactobacillus plantarum is replaced with Bacillus coagulans, or handle
The amount of Bacillus coagulans replaces with Lactobacillus plantarum) tunning carry out such as examples of implementation 4
Same experiment, the formula of use is consistent, it is thus achieved that result as follows: condense bud when individually using
The when of spore bacillus fermentation product, the average daily gain (kg/d) of experimental group is 510.14 ±
140.91, diarrhoea is respectively 6.2%；When individually using Lactobacillus plantarum tunning when,
The average daily gain (kg/d) of experimental group is 494.14 ± 134.91, and diarrhoea is respectively 5.1%.
Thus Bacillus coagulans fermentation and the product of Lactobacillus plantarum mixed fermentation are described, it is possible not only to
Wanting improving average daily gain, and reduce diarrhea rate, further illustrating them has the most collaborative
Potentiation.Thus, the two the most together with the use of, be mixed together fermentation and can play him
Preferably replace the effect of antibiotic.
Claims (4)
1. a production method for ablactational baby pig compound micro-ecological preparation, the method sequentially includes the following steps:
(1) preserving number is Bacillus coagulans (Bacillus coagulans) the liquid seeds cultivation of ACCC No.00403 bacterial strain: Bacillus coagulans slant strains accessed in triangular flask culture medium, 500ml triangular flask culture medium loading amount is 100ml, through 35～38 DEG C, 180～200rpm shaking tables become triangular flask liquid seeds after cultivating 14～18h, standby；The seed culture medium of described triangular flask is (g/L): lactose 1, peptone 1, beef extract 0.5, sodium chloride 0.5, and adjusting pH is 7.0～7.5,121 DEG C of sterilizing 15min；
(2) Bacillus coagulans aerobic solids fermentation culture: solid fermentation culture medium is bean cake, the water adding bean cake weight 0.6 times is formulated, by every bottle of triangular flask liquid seeds of step (1) by every bottle of seed: the ratio inoculation of 20～30 kilograms of bean cake in terms of solid content weight, without sterilizing, after loading koji tray is 5～7cm to fermentation materials thickness, in 35～38 DEG C of room temperature condition bottom fermentations cultivations 20～24h, standby；
(3) preserving number be ACCC No.11016 bacterial strain Lactobacillus plantarum (Lactobacillus plantarum) seed liquor cultivate: in 500ml triangular flask load 300ml liquid MRS culture medium, access Lactobacillus plantarum strain, after 35～38 DEG C are cultivated 12～14h, in 0～4 DEG C of preservation, standby；Described MRS culture medium is (g/L): glucose 2, peptone 1, beef extract 1, yeast extract 0.5, dipotassium hydrogen phosphate 0.2, dibasic ammonium citrate 0.2, sodium acetate 0.5, tween 0.1, magnesium sulfate 0.058, manganese sulfate 0.025, adjusting pH is 5.8～6.2,121 DEG C of sterilizing 15min；
(4) Bacillus coagulans and the fermentation of Lactobacillus plantarum hybrid solid: hybrid solid fermentation medium is: defatted rice bran: step (2) Bacillus coagulans aerobic solids fermentation culture medium (based on bean cake weight before fermentation): calcium carbonate by weight 70～90: 10～30: 4～10 proportions forms, and by 0.5～0.7 times of addition tap water of the weight of defatted rice bran in this fermentation medium, without sterilizing, Lactobacillus plantarum seed liquor by step (3), by 1%～3% inoculation of the weight of defatted rice bran in this mixed fermentive culture medium, mix thoroughly, directly proceed in plastic bag, tighten plastic bag, drive the air in bag away as far as possible, fermentation is stopped after continuing to cultivate 30～36h under 35～38 DEG C of condition of culture；After fermented product being dried, is ground into 40 mesh under the conditions of not higher than 50 DEG C, check, be packaged into product.
2. the purposes being carried out substitute antibiotics by the compound micro-ecological preparation of method production described in claim 1 in preparation ablactational baby pig feedstuff.
Purposes the most according to claim 2, wherein, described feedstuff consists of the following composition: Semen Maydis 60.8%, bean cake 15.0%, puffing Semen sojae atricolor 10.0%, fish flour 5.0%, whey powder 6.0%, calcium hydrogen phosphate 1.2%, stone powder 0.6%, salt 0.3%, lysine 0.1%, premix material 1.0%, the compound micro-ecological preparation that the method as claimed in claim 1 of 0.4 produces.
4. an ablactational baby pig mixed feed, wherein, this feedstuff is become to be grouped into by following: Semen Maydis 60.8%, bean cake 15.0%, puffing Semen sojae atricolor 10.0%, fish flour 5.0%, whey powder 6.0%, calcium hydrogen phosphate 1.2%, stone powder 0.6%, salt 0.3%, lysine 0.1%, premix material 1.0%, the compound micro-ecological preparation that the method as claimed in claim 1 of 0.4% produces.
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| CN106924477A (en) * | 2017-03-17 | 2017-07-07 | 哈尔滨中科生物工程有限公司 | A kind of compound Chinese medicine fermentation preparation of mixed fermentation production and preparation method thereof |
| CN107594086A (en) * | 2017-09-28 | 2018-01-19 | 北京农学院 | A kind of composite microbial compositions and feed for nursery-age pig |
| CN109362942A (en) * | 2018-12-29 | 2019-02-22 | 广东旺大集团股份有限公司 | A kind of wean sucking pig fermented feed and preparation method thereof |
| CN109699812A (en) * | 2019-02-24 | 2019-05-03 | 浙江省农业科学院 | Solid state fermentation produces feeding saccharomyces cerevisiae-lactobacillus plantarum product mix method |
| CN109770077A (en) * | 2019-01-31 | 2019-05-21 | 天津市畜牧兽医研究所 | Lactobacillus plantarum ACCC11016 is improving the application in alfalfa ensilage crude fat content |
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| CN109770077A (en) * | 2019-01-31 | 2019-05-21 | 天津市畜牧兽医研究所 | Lactobacillus plantarum ACCC11016 is improving the application in alfalfa ensilage crude fat content |
| CN109699812A (en) * | 2019-02-24 | 2019-05-03 | 浙江省农业科学院 | Solid state fermentation produces feeding saccharomyces cerevisiae-lactobacillus plantarum product mix method |
| CN116491577A (en) * | 2023-05-08 | 2023-07-28 | 宁夏大学 | High-activity probiotic composite microecological preparation and preparation method and application thereof |
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Application publication date: 20160921 |