CN100374544C - Ecological microorganism preparation for animals and birds and method for preparing same - Google Patents
Ecological microorganism preparation for animals and birds and method for preparing same Download PDFInfo
- Publication number
- CN100374544C CN100374544C CNB2006100100221A CN200610010022A CN100374544C CN 100374544 C CN100374544 C CN 100374544C CN B2006100100221 A CNB2006100100221 A CN B2006100100221A CN 200610010022 A CN200610010022 A CN 200610010022A CN 100374544 C CN100374544 C CN 100374544C
- Authority
- CN
- China
- Prior art keywords
- mixture
- ratio
- under
- water
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims description 19
- 238000000034 method Methods 0.000 title abstract description 3
- 241001465754 Metazoa Species 0.000 title abstract 4
- 244000005700 microbiome Species 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- 239000000203 mixture Substances 0.000 claims abstract description 47
- 239000008247 solid mixture Substances 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 19
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 19
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 15
- 235000009566 rice Nutrition 0.000 claims abstract description 15
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 15
- 238000010298 pulverizing process Methods 0.000 claims abstract description 14
- 241000894006 Bacteria Species 0.000 claims abstract description 12
- 241000186226 Corynebacterium glutamicum Species 0.000 claims abstract description 12
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 12
- 244000168141 Geotrichum candidum Species 0.000 claims abstract description 12
- 235000017388 Geotrichum candidum Nutrition 0.000 claims abstract description 12
- 230000029087 digestion Effects 0.000 claims abstract description 12
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
- 244000144972 livestock Species 0.000 claims description 15
- 244000144977 poultry Species 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 15
- 241000209094 Oryza Species 0.000 claims description 13
- 239000010903 husk Substances 0.000 claims description 13
- 239000002068 microbial inoculum Substances 0.000 claims description 12
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 10
- 230000000813 microbial effect Effects 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 8
- 238000011218 seed culture Methods 0.000 claims description 8
- 210000000582 semen Anatomy 0.000 claims description 7
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 6
- 239000010452 phosphate Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 230000007062 hydrolysis Effects 0.000 claims description 4
- 238000006460 hydrolysis reaction Methods 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract description 3
- 240000007594 Oryza sativa Species 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 238000009395 breeding Methods 0.000 abstract 2
- 230000001488 breeding effect Effects 0.000 abstract 2
- 238000001035 drying Methods 0.000 abstract 2
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 240000008042 Zea mays Species 0.000 abstract 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 235000005822 corn Nutrition 0.000 abstract 1
- 235000013312 flour Nutrition 0.000 abstract 1
- 230000036039 immunity Effects 0.000 abstract 1
- 206010012735 Diarrhoea Diseases 0.000 description 4
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 235000012631 food intake Nutrition 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 235000019733 Fish meal Nutrition 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 239000005862 Whey Substances 0.000 description 2
- 102000007544 Whey Proteins Human genes 0.000 description 2
- 108010046377 Whey Proteins Proteins 0.000 description 2
- 241000482268 Zea mays subsp. mays Species 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 235000008939 whole milk Nutrition 0.000 description 2
- 238000010171 animal model Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Abstract
The present invention provides a micro-ecological bacterial agent for animals and birds and a preparing method. The micro-ecological bacterial agent is composed of mixture of group A with the weight ratio of 65 to 70 percent and mixture of group B with the weight ratio of 30 to 35 percent, wherein the mixture of group A is solid mixture of wheat bran, bean pulp and rice hull; firstly, the solid mixture is added with water for breeding secondary level seed liquid of candida utilis and geotrichum candidum, and then after culturing, drying and pulverizing, the mixture of group A is obtained. The mixture of group B is the mixture of high-temperature bean pulp, wheat bran, corn flour and rice hull; firstly, the mixture is added with water for breeding the seed liquid of corynebacterium glutamicum, digesting bacterium lacticum and bacillus subtilis liquid, and then after culturing, drying and pulverizing, the mixture of group B is obtained. The product of the present invention can strengthen the digestion and absorption capability of animals and birds, strengthen organism immunity, and effectively promote the growth of animals and birds.
Description
(1) technical field
What the present invention relates to is a kind of microbial preparation, the invention still further relates to the preparation method of this microbial preparation.
(2) background technology
Can cause the diarrhoea of livestock and poultry for the common feed of livestock and poultry feeding after weaning,, influence the food consumption of livestock and poultry, cause the weightening finish of livestock and poultry slow etc. symptom.The major cause that causes this phenomenon is that the intravital beneficial flora of livestock and poultry is unbalance.Also do not have at present a kind ofly can regulate livestock and poultry, particularly the fodder additives of beneficial flora in the livestock and poultry young baby body.
(3) summary of the invention
The object of the present invention is to provide and a kind ofly can effectively reduce the diarrhea rate of livestock and poultry, improve food consumption, improve the livestock and poultry microecological microbial agent of the weight that increases day by day.The present invention also aims to provide a kind of preparation method of livestock and poultry microecological microbial agent.
The object of the present invention is achieved like this: the related per-cent among the present invention is the mass/volume ratio except that other has indicating.
It is that weight ratio is A group mixture and the 30-35%B group mixture composition of 65-70%; Wherein A group mixture is that weight ratio is a wheat bran: dregs of beans: rice husk=(90-100): (8-10): solid mixture (1-3), add the water that accounts for solid mixture weight 15-20%, be added with 0.1% the potassium primary phosphate that accounts for water and 0.05% sal epsom in the described water that adds solid mixture to, its per-cent is the mass/volume ratio, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, at 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 45-50 ℃ of temperature, be dried to water content below 15%, the mixture that pulverizing obtains; B group mixture is that weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): solid mixture (1-2), add the water that accounts for solid mixture weight 15-20%, be added with 0.5% the sodium-chlor that accounts for water in the described water that adds solid mixture to, its per-cent is the mass/volume ratio, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 45-50 ℃ of temperature, the mixture that pulverizing obtains.
Product of the present invention is to adopt such method to prepare:
1, the preparation of A group mixture
1.1, with 8 degree Beaume worts 121 ℃ the sterilization 20 minutes, be cooled to 28 ℃, ratio by weight 10% inserts first order seed, constant temperature culture is 18 hours under 28 ℃ of temperature, 10% ratio inserts secondary seed by weight again, enlarged culturing obtained secondary seed solution in 24 hours under 28 ℃ of temperature, and described seed is respectively Candida utilis, geotrichum candidum seed;
1.2, according to weight ratio is wheat bran: dregs of beans: rice husk=(90-100): (8-10): mixed (1-3) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.1% the potassium primary phosphate that accounts for water and 0.05% sal epsom in the described water that adds solid mixture to, its per-cent is the mass/volume ratio, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, at 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 40-60 ℃ of temperature, be dried to water content below 15%, pulverizing obtains A group mixture;
2, the preparation of B group mixture:
2.1 according to glucose 1%, hydrolysis bean cake powder 1%, VB
2The mixed of 5mg/L, 0.5% sodium-chlor and excess water is made liquid seed culture medium, per-cent wherein is weight percentage, liquid seed culture medium was sterilized 20 minutes under 121 ℃ of temperature, be cooled to 35 ℃, according to weight ratio is 10% ratio inoculation first order seed, 35 ℃ of constant temperature culture 20 hours, according to weight ratio is that 10% ratio inserts secondary seed, 35 ℃ of enlarged culturing 36 hours, obtain secondary seed solution, described seed is respectively Corynebacterium glutamicum, digestion Bacterium lacticum, subtilis;
2.2 according to weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): mixed (1-2) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.5% the sodium-chlor that accounts for water in the described water that adds solid mixture to, its per-cent is the mass/volume ratio, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 40-60 ℃ of temperature, pulverizing obtains B group mixture;
3) finished product preparation
Be that the A group mixture of 65-70% is packed with the ratio thorough mixing final vacuum of the B group mixture of 30-35% and made finished product according to weight ratio.
It is carrier with high temperature dregs of beans, wheat bran, Semen Maydis powder, rice husk and adequate nutrition salt that the present invention adopts biotechnology, cultivates 5 probiotics under optimal temperature respectively, is mixed in proportion packing after the pulverizing and makes product.Product of the present invention can strengthen the ability of the digesting and assimilating while enhancing body immunizing power of livestock and poultry, can effectively promote the growth of livestock and poultry.
Prove effect of the present invention below by concrete experiment
(1). experimental animal is selected:
Select each 20 of wean one all piglets, be divided into two groups, establish test group and control group.
(2). test method:
Weaning begins to enter preliminary trial period the last week, weans and weighs in back second day, and grouping enters trial period.
(3). feed form and feed formulation: adopt the particulate material free choice feeding, addition 10% adds after eating up immediately, can not fracture.
Control group: popcorn 50%, expanded soybean 15.5%, whole milk powder 8%, whey powder 7%, swelling soya dreg 6%, fish meal 5.5%, sucrose 3%, additive 5%.
Test group: popcorn 50%, expanded soybean 15.5%, whole milk powder 8%, whey powder 7%, microecological microbial agent 6%, fish meal 5.5%, sucrose 3%, additive 5%
(4). test-results (10 days)
| Just average heavy Kg | The average heavy Kg in end | Average daily gain g | The material anharmonic ratio | The diarrhoea ratio |
| Control group 8.9 | 11.8 | 290 | 1.4∶1 | 1∶10 |
| Test group 8.85 | 12.6 | 375 | 1.35∶1 | Do not have |
(5). interpretation of result
Product of the present invention can effectively reduce the diarrhea rate of piglet, improves food consumption, improves to increase day by day weight more than 20%.
(4) embodiment
For a more detailed description to the present invention for example below:
Embodiment one
5 probiotics that the present invention adopts are all purchased in Chinese agriculture microbial strains preservation administrative center, are respectively: Candida utilis (ACCC20060), geotrichum candidum (ACCC20016), Corynebacterium glutamicum (ACCC10159), digestion Bacterium lacticum (ACCC10173), subtilis (ACCC10114).
1, the preparation of A group mixture
1.1, with 8 degree Beaume worts 121 ℃ the sterilization 20 minutes, be cooled to 28 ℃, ratio by weight 10% inserts first order seed, constant temperature culture is 18 hours under 28 ℃ of temperature, 10% ratio inserts secondary seed by weight again, enlarged culturing obtained secondary seed solution in 24 hours under 28 ℃ of temperature, and described seed is respectively Candida utilis, geotrichum candidum seed;
1.2, according to weight ratio is wheat bran: dregs of beans: rice husk=(90-100): (8-10): mixed (1-3) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.1% potassium primary phosphate in the water, 0.05% sal epsom, its per-cent is the mass/volume ratio, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, at 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 40-60 ℃ of temperature, be dried to water content below 15%, pulverizing obtains A group mixture;
2, the preparation of B group mixture:
2.1 according to glucose 1%, hydrolysis bean cake powder 1%, VB
2The mixed of 5mg/L, 0.5% sodium-chlor and excess water is made liquid seed culture medium, per-cent wherein is weight percentage, liquid seed culture medium was sterilized 20 minutes under 121 ℃ of temperature, be cooled to 35 ℃, according to weight ratio is 10% ratio inoculation first order seed, 35 ℃ of constant temperature culture 20 hours, according to weight ratio is that 10% ratio inserts secondary seed, 35 ℃ of enlarged culturing 36 hours, obtain secondary seed solution, described seed is respectively Corynebacterium glutamicum, digestion Bacterium lacticum, subtilis;
2.2 according to weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): mixed (1-2) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.5% sodium-chlor in the water, its per-cent is the mass/volume ratio, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 40-60 ℃ of temperature, pulverizing obtains B group mixture;
3, finished product preparation
Be that the A group mixture of 65-70% is packed with the ratio thorough mixing final vacuum of the B group mixture of 30-35% and made finished product according to weight ratio.
Embodiment two
1, the preparation of A group mixture
1.1, with 8 degree Beaume worts 121 ℃ the sterilization 20 minutes, be cooled to 28 ℃, ratio by weight 10% inserts first order seed, constant temperature culture is 18 hours under 28 ℃ of temperature, 10% ratio inserts secondary seed by weight again, enlarged culturing obtained secondary seed solution in 24 hours under 28 ℃ of temperature, and described seed is respectively Candida utilis, geotrichum candidum seed;
1.2, according to weight ratio is wheat bran: dregs of beans: the mixed of rice husk=90: 8: 2 evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.1% potassium primary phosphate in the water, 0.05% sal epsom, its per-cent is the mass/volume ratio, under 100 ℃ temperature, steam 35 minutes, be cooled to 28 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of 20% Candida utilis and 20% adds microbial inoculum, at 28 ℃, suitably cultivated 35 hours under the airy condition, under 50 ℃ of temperature, be dried to water content below 15%, pulverizing obtains A group mixture;
2, the preparation of B group mixture:
2.1 according to glucose 1%, hydrolysis bean cake powder 1%, VB
2The mixed of 5mg/L, 0.5% sodium-chlor and excess water is made liquid seed culture medium, per-cent wherein is weight percentage, liquid seed culture medium was sterilized 20 minutes under 121 ℃ of temperature, be cooled to 35 ℃, according to weight ratio is 10% ratio inoculation first order seed, 35 ℃ of constant temperature culture 20 hours, according to weight ratio is that 10% ratio inserts secondary seed, 35 ℃ of enlarged culturing 36 hours, obtain secondary seed solution, described seed is respectively Corynebacterium glutamicum, digestion Bacterium lacticum, subtilis;
2.2 according to weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=20: 60: 18: 2 mixed evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.5% sodium-chlor in the water, its per-cent is the mass/volume ratio, under 100 ℃ of temperature, steam 25-35 minute, be cooled to 35 ℃, be Corynebacterium glutamicum by weight, each ratio of 15% of digestion Bacterium lacticum and subtilis liquid seeds liquid adds microbial inoculum, under 35 ℃ of suitable airy conditions, cultivated 48 hours, be dried to water content below 15% under 50 ℃ of temperature, pulverizing obtains B group mixture;
3, finished product preparation
To be 65% A group mixture with the ratio thorough mixing final vacuum of 35% B group mixture pack makes finished product according to weight ratio.
Claims (2)
1. livestock and poultry microecological microbial agent is characterized in that: it is that weight ratio is the A group mixture and 30-35%B group mixture composition of 65-70%; Wherein A group mixture is that weight ratio is a wheat bran: dregs of beans: rice husk=(90-100): (8-10): solid mixture (1-3), add the water that accounts for solid mixture weight 15-20%, be added with 0.1% the potassium primary phosphate that accounts for water and 0.05% sal epsom in the described water that adds solid mixture to, its per-cent is the mass/volume ratio, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, at 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 45-50 ℃ of temperature, be dried to water content below 15%, the mixture that pulverizing obtains; B group mixture is that weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): solid mixture (1-2), add the water that accounts for solid mixture weight 15-20%, be added with 0.5% the sodium-chlor that accounts for water in the described water that adds solid mixture to, its per-cent is the mass/volume ratio, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 45-50 ℃ of temperature, the mixture that pulverizing obtains.
2. the preparation method of a livestock and poultry microecological microbial agent is characterized in that:
(1), the preparation of A group mixture
(1.1), 8 degree Beaume worts were sterilized 20 minutes at 121 ℃, be cooled to 28 ℃, ratio by weight 10% inserts first order seed, constant temperature culture is 18 hours under 28 ℃ of temperature, 10% ratio inserts secondary seed by weight again, enlarged culturing obtained secondary seed solution in 24 hours under 28 ℃ of temperature, and described seed is respectively Candida utilis, geotrichum candidum seed;
(1.2), according to weight ratio is wheat bran: dregs of beans: rice husk=(90-100): (8-10): mixed (1-3) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.1% the potassium primary phosphate that accounts for water and 0.05% sal epsom in the described water that adds solid mixture to, its per-cent is the mass/volume ratio, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, at 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 40-60 ℃ of temperature, be dried to water content below 15%, pulverize and obtain A group mixture;
(2), the preparation of B group mixture:
(2.1) according to glucose 1%, hydrolysis bean cake powder 1%, VB
2The mixed of 5mg/L, 0.5% sodium-chlor and excess water is made liquid seed culture medium, per-cent wherein is weight percentage, liquid seed culture medium was sterilized 20 minutes under 121 ℃ of temperature, be cooled to 35 ℃, according to weight ratio is 10% ratio inoculation first order seed, 35 ℃ of constant temperature culture 20 hours, according to weight ratio is that 10% ratio inserts secondary seed, 35 ℃ of enlarged culturing 36 hours, obtain secondary seed solution, described seed is respectively Corynebacterium glutamicum, digestion Bacterium lacticum, subtilis;
(2.2) be the high temperature dregs of beans according to weight ratio: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): mixed (1-2) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.5% the sodium-chlor that accounts for water in the described water that adds solid mixture to, its per-cent is the mass/volume ratio, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 40-60 ℃ of temperature, pulverizing obtains B group mixture;
(3), finished product preparation
Be that the A group mixture of 65-70% is packed with the ratio thorough mixing final vacuum of the B group mixture of 30-35% and made finished product according to weight ratio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100100221A CN100374544C (en) | 2006-05-10 | 2006-05-10 | Ecological microorganism preparation for animals and birds and method for preparing same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100100221A CN100374544C (en) | 2006-05-10 | 2006-05-10 | Ecological microorganism preparation for animals and birds and method for preparing same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1844349A CN1844349A (en) | 2006-10-11 |
| CN100374544C true CN100374544C (en) | 2008-03-12 |
Family
ID=37063319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100100221A Active CN100374544C (en) | 2006-05-10 | 2006-05-10 | Ecological microorganism preparation for animals and birds and method for preparing same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100374544C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757277B (en) * | 2012-07-31 | 2013-12-04 | 长春华御实业集团有限公司 | Paddy biofertilizer, preparation method thereof and bacillus subtilis |
| CN104605181A (en) * | 2015-01-06 | 2015-05-13 | 铁岭新东方饲料有限公司 | Environment-friendly compound feed for pig and preparation method of environment-friendly compound feed |
| CN104839454B (en) * | 2015-05-28 | 2018-02-16 | 河南双成生物科技有限公司 | A kind of high protein feed production method rich in valine |
| CN105076708A (en) * | 2015-09-22 | 2015-11-25 | 河南科技大学 | Poultry yeast culture and preparation method thereof |
| CN108911879A (en) * | 2018-08-23 | 2018-11-30 | 北京市植物保护站 | A kind of bioactive functions reduction disinfection particle and sterilization method |
| CN114921399A (en) * | 2022-06-30 | 2022-08-19 | 哈尔滨市灵扩科技发展有限公司 | Preparation method of efficient culture livestock and poultry intestinal probiotics |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060621C (en) * | 1997-10-24 | 2001-01-17 | 天津市农业生物工程研究中心 | Biogenic additives for fermentation of stalks and preparation thereof |
| CN1552233A (en) * | 2003-05-26 | 2004-12-08 | 哈尔滨中冠生物工程有限公司 | Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation |
-
2006
- 2006-05-10 CN CNB2006100100221A patent/CN100374544C/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060621C (en) * | 1997-10-24 | 2001-01-17 | 天津市农业生物工程研究中心 | Biogenic additives for fermentation of stalks and preparation thereof |
| CN1552233A (en) * | 2003-05-26 | 2004-12-08 | 哈尔滨中冠生物工程有限公司 | Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation |
Non-Patent Citations (2)
| Title |
|---|
| 微生物在饲料开发中的应用. 谭周进等.常德高等专科学校学报，第11卷第2期. 1999 * |
| 饲用微生态制剂的应用研究进展. 海存秀.青海大学学报（自然科学版），第24卷第1期. 2006 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1844349A (en) | 2006-10-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102168045B (en) | Bacillus subtilis preparation and preparation method thereof | |
| CN101558823B (en) | Fermented liquid feed for Pig and poultry as well as preparation method and application thereof | |
| CN102232998B (en) | Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof | |
| CN103667222B (en) | Feed compound enzyme-containing dedicated enzyme for growing pigs and preparation method of feed compound enzyme-containing dedicated enzyme | |
| CN102488084B (en) | Method for preparing protein feed by fermentation on straw and cake using aerobic mixed bacteria | |
| CN100542417C (en) | Pig is used fitting-type concentrated feed and production technology and feeding method | |
| CN101019609A (en) | Solid fermentation process for producing natto bacillus feed additive with bean dregs | |
| CN104054966B (en) | Pig is fattened with biological fodder and its preparation method in a kind of Wuzhi Mountain | |
| CN104970252A (en) | Fermented liquid suckling pig feed and preparation method thereof | |
| CN103829042B (en) | A kind of production method of polyvitmin active protein cassava feed | |
| CN102559545A (en) | Composite micro-ecological preparation for aquatic product and premix thereof | |
| CN102293346A (en) | Fermented feed for piglets and preparation method thereof | |
| CN104186934A (en) | Creep feed specially used for piglet and preparation method thereof | |
| CN106333060A (en) | Microbial fermentation feed and preparation method and application thereof | |
| CN100374544C (en) | Ecological microorganism preparation for animals and birds and method for preparing same | |
| CN107683972B (en) | Improve the fermented feed and its preparation method and application of milking sow production performance | |
| CN108576374A (en) | Fermented feed raw material and preparation method thereof, purposes and the complete feed comprising it | |
| CN101120719B (en) | Microorganism ferment purple sweet potato and preparation method thereof | |
| CN103859155A (en) | Refining method for probiotics-containing cassava feed | |
| CN105941899A (en) | Production method for compound micro-ecological preparation for weaned piglets | |
| CN106234755A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with bagasse | |
| CN1934989A (en) | Microbial fodder additive | |
| CN102960538B (en) | Unicellular protein feed prepared from fermented dragon fruit peel and production method of unicellular protein feed | |
| CN101664101A (en) | Method for preparing biological fodder for pigs | |
| CN105994959A (en) | Preparation method of lactobacillus acidophilus feed additive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: HARBIN QINGHE SCIENCE AND TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: CHEN CHENG Effective date: 20091113 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20091113 Address after: No. 28, Honghu Road, Yingbin Road, Harbin Development Zone, Heilongjiang, China Patentee after: Harbin Qinghe Technology Co., Ltd. Address before: No. 3, unit 35, building 703, Minjiang District, Nangang District, Heilongjiang, Harbin Patentee before: Chen Cheng |
|
| C56 | Change in the name or address of the patentee |
Owner name: GUSHI AGRICULTURAL AND PASTORAL GROUP CO., LTD. Free format text: FORMER NAME: HARBIN QINGHE TECHNOLOGY CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 150090 No. 28, Honghu Road, Yingbin Road, Harbin Development Zone, Heilongjiang, China Patentee after: Gushi Agricultural and Pastoral Group Co., Ltd. Address before: 150090 No. 28, Honghu Road, Yingbin Road, Harbin Development Zone, Heilongjiang, China Patentee before: Harbin Qinghe Technology Co., Ltd. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 150090 No. 28, Honghu Road, Yingbin Road, Harbin Development Zone, Heilongjiang, China Patentee after: Gushi biological Group Co., Ltd Address before: 150090 No. 28, Honghu Road, Yingbin Road, Harbin Development Zone, Heilongjiang, China Patentee before: GUSHI AGRICULTURE AND ANIMAL HUSBANDRY GROUP Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |