CN1844349A - Ecological microorganism preparation for animals and birds and method for preparing same - Google Patents
Ecological microorganism preparation for animals and birds and method for preparing same Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 241001465754 Metazoa Species 0.000 title abstract 3
- 238000000034 method Methods 0.000 title description 3
- 244000005700 microbiome Species 0.000 title 1
- 239000000203 mixture Substances 0.000 claims abstract description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 15
- 239000010903 husk Substances 0.000 claims abstract description 15
- 235000009566 rice Nutrition 0.000 claims abstract description 15
- 241000186226 Corynebacterium glutamicum Species 0.000 claims abstract description 12
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 12
- 230000029087 digestion Effects 0.000 claims abstract description 12
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 239000008247 solid mixture Substances 0.000 claims description 36
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 17
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 17
- 244000144972 livestock Species 0.000 claims description 17
- 244000144977 poultry Species 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 15
- 241000209094 Oryza Species 0.000 claims description 13
- 238000010298 pulverizing process Methods 0.000 claims description 13
- 235000015099 wheat brans Nutrition 0.000 claims description 13
- 239000002068 microbial inoculum Substances 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 11
- 244000168141 Geotrichum candidum Species 0.000 claims description 11
- 235000017388 Geotrichum candidum Nutrition 0.000 claims description 11
- 230000000813 microbial effect Effects 0.000 claims description 11
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 8
- 238000011218 seed culture Methods 0.000 claims description 8
- 210000000582 semen Anatomy 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 230000007062 hydrolysis Effects 0.000 claims description 4
- 238000006460 hydrolysis reaction Methods 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 abstract description 5
- 235000010469 Glycine max Nutrition 0.000 abstract description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract description 3
- 240000007594 Oryza sativa Species 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 241000186660 Lactobacillus Species 0.000 abstract 1
- 240000008042 Zea mays Species 0.000 abstract 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 235000013312 flour Nutrition 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 235000009973 maize Nutrition 0.000 abstract 1
- 206010012735 Diarrhoea Diseases 0.000 description 4
- 239000000654 additive Substances 0.000 description 3
- 235000012631 food intake Nutrition 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 235000019733 Fish meal Nutrition 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 239000005862 Whey Substances 0.000 description 2
- 102000007544 Whey Proteins Human genes 0.000 description 2
- 108010046377 Whey Proteins Proteins 0.000 description 2
- 241000482268 Zea mays subsp. mays Species 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 235000008939 whole milk Nutrition 0.000 description 2
- 238000010171 animal model Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
This invention provides a micro-ecological bacterial agent of domestic animals and the preparation method thereof. The agent is composed by 65-70% mixture A and 30-35% mixture B by weight ratio, wherein: the mixture A is obtained following the steps of mixing water with solid compounds of bran, soya bean watse, and rice husk, inoculated with second strain seed liquid of Candida utilis and Cryytococcus neoformans, cultured, dryed and disintegrated; the mixture B is obtained following the steps of mixing water with solid compounds of soya bean watse, bran, maize flour and rice husk,inoculated with the seed liquid of Corynebacterium glutamicum, Lactobacillus. Alimentarius, and bacillus subtilis, cultured, dryed and disintegrated. The product of this invention is characterized in that it can improve the digestion and absorption capacity, enhance the body immune competence and efficiently stimulate the growth of domestic animals.
Description
(1) technical field
What the present invention relates to is a kind of microbial preparation, the invention still further relates to the preparation method of this microbial preparation.
(2) background technology
Can cause the diarrhoea of livestock and poultry for the common feed of livestock and poultry feeding after weaning,, influence the food consumption of livestock and poultry, cause the weightening finish of livestock and poultry slow etc. symptom.The major cause that causes this phenomenon is that the intravital beneficial flora of livestock and poultry is unbalance.Also do not have at present a kind ofly can regulate livestock and poultry, particularly the fodder additives of beneficial flora in the livestock and poultry young baby body.
(3) summary of the invention
The object of the present invention is to provide and a kind ofly can effectively reduce the diarrhea rate of livestock and poultry, improve food consumption, improve the livestock and poultry microecological microbial agent of the weight that increases day by day.The present invention also aims to provide a kind of preparation method of livestock and poultry microecological microbial agent.
The object of the present invention is achieved like this: the related per-cent among the present invention is the mass/volume ratio except that other has indicating.
It is that weight ratio is A group mixture and the 30-35%B group mixture composition of 65-70%; Wherein A group mixture is that weight ratio is a wheat bran: dregs of beans: rice husk=(90-100): (8-10): solid mixture (1-3), add the water that accounts for solid mixture weight 15-20%, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 45-50 ℃ of temperature, be dried to the mixture that water content 15% below, pulverizing obtain; B group mixture is that weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): solid mixture (1-2), add the water that accounts for solid mixture weight 15-20%, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 45-50 ℃ of temperature, the mixture that pulverizing obtains.
Product of the present invention is to adopt such method to prepare:
1, the preparation of A group mixture
1.1, with 8 degree Beaume worts 121 ℃ the sterilization 20 minutes, be cooled to 28 ℃, ratio by weight 10% inserts first order seed, constant temperature culture is 18 hours under 28 ℃ of temperature, 10% ratio inserts secondary seed by weight again, enlarged culturing obtained secondary seed solution in 24 hours under 28 ℃ of temperature, and described seed is respectively Candida utilis, geotrichum candidum seed;
1.2, according to weight ratio is wheat bran: dregs of beans: rice husk=(90-100): (8-10): mixed (1-3) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, at 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 40-60 ℃ of temperature, be dried to water content below 15%, pulverizing obtains A group mixture;
2, the preparation of B group mixture:
2.1 according to glucose 1%, hydrolysis bean cake powder 1%, 5mgVB
2The mixed of/L, 0.5% sodium-chlor and excess water is made liquid seed culture medium, per-cent wherein is weight percentage, liquid seed culture medium was sterilized 20 minutes under 121 ℃ of temperature, be cooled to 35 ℃, according to weight ratio is 10% ratio inoculation first order seed, 35 ℃ of constant temperature culture 20 hours, according to weight ratio is that 10% ratio inserts secondary seed, 35 ℃ of enlarged culturing 36 hours, obtain secondary seed solution, described seed is respectively Corynebacterium glutamicum, digestion Bacterium lacticum, subtilis;
2.2 according to weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): mixed (1-2) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 40-60 ℃ of temperature, pulverizing obtains B group mixture;
3, finished product preparation
Be that the A group mixture of 65-70% is packed with the ratio thorough mixing final vacuum of the B group mixture of 30-35% and made finished product according to weight ratio.
Method of the present invention can also comprise:
1, add 0.1% potassium primary phosphate that accounts for water in the preparation process of A group mixture in the water that adds solid mixture to, 0.05% sal epsom, its per-cent are the mass/volume ratio.
2, add 0.5% sodium-chlor that accounts for water in the preparation process of B group mixture in the water that adds solid mixture to, its per-cent is the mass/volume ratio.
It is carrier with high temperature dregs of beans, wheat bran, Semen Maydis powder, rice husk and adequate nutrition salt that the present invention adopts biotechnology, cultivates 5 probiotics under optimal temperature respectively, is mixed in proportion packing after the pulverizing and makes product.Product of the present invention can strengthen the ability of the digesting and assimilating while enhancing body immunizing power of livestock and poultry, can effectively promote the growth of livestock and poultry.
Prove effect of the present invention below by concrete experiment
(1). experimental animal is selected:
Select each 20 of wean one all piglets, be divided into two groups, establish test group and control group.
(2). test method:
Weaning begins to enter preliminary trial period the last week, weans and weighs in back second day, and grouping enters trial period.
(3). feed form and feed formulation: adopt the particulate material free choice feeding, addition 10% adds after eating up immediately, can not fracture.
Control group: popcorn 50%, expanded soybean 15.5%, whole milk powder 8%, whey powder 7%, swelling soya dreg 6%, fish meal 5.5%, sucrose 3%, additive 5%.
Test group: popcorn 50%, expanded soybean 15.5%, whole milk powder 8%, whey powder 7%, microecological microbial agent 6%, fish meal 5.5%, sucrose 3%, additive 5%
(4). test-results (10 days)
| Just average heavy Kg | The average heavy Kg in end | Average daily gain g | The material anharmonic ratio | The diarrhoea ratio |
| Control group 8.9 | 11.8 | 290 | 1.4∶1 | 1∶10 |
| Test group 8.85 | 12.6 | 375 | 1.35∶1 | Do not have |
(5). interpretation of result
Product of the present invention can effectively reduce the diarrhea rate of piglet, improves food consumption, improves to increase day by day weight more than 20%.
(4) embodiment
For a more detailed description to the present invention for example below:
Embodiment one
5 probiotics that the present invention adopts are all purchased in Chinese agriculture microbial strains preservation administrative center, are respectively: Candida utilis (ACCC20060), geotrichum candidum (ACCC20016), Corynebacterium glutamicum (ACCC10159), digestion Bacterium lacticum (ACCC10173), subtilis (ACCC10114).
1, the preparation of A group mixture
1.1, with 8 degree Beaume worts 121 ℃ the sterilization 20 minutes, be cooled to 28 ℃, ratio by weight 10% inserts first order seed, constant temperature culture is 18 hours under 28 ℃ of temperature, 10% ratio inserts secondary seed by weight again, enlarged culturing obtained secondary seed solution in 24 hours under 28 ℃ of temperature, and described seed is respectively Candida utilis, geotrichum candidum seed;
1.2, according to weight ratio is wheat bran: dregs of beans: rice husk=(90-100): (8-10): mixed (1-3) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.1% potassium primary phosphate in the water, 0.05% sal epsom, its per-cent is the mass/volume ratio, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, at 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 40-60 ℃ of temperature, be dried to water content below 15%, pulverizing obtains A group mixture;
2, the preparation of B group mixture:
2.1 according to glucose 1%, hydrolysis bean cake powder 1%, 5mgVB
2The mixed of/L, 0.5% sodium-chlor and excess water is made liquid seed culture medium, per-cent wherein is weight percentage, liquid seed culture medium was sterilized 20 minutes under 121 ℃ of temperature, be cooled to 35 ℃, according to weight ratio is 10% ratio inoculation first order seed, 35 ℃ of constant temperature culture 20 hours, according to weight ratio is that 10% ratio inserts secondary seed, 35 ℃ of enlarged culturing 36 hours, obtain secondary seed solution, described seed is respectively Corynebacterium glutamicum, digestion Bacterium lacticum, subtilis;
2.2 according to weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): mixed (1-2) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.5% sodium-chlor in the water, its per-cent is the mass/volume ratio, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 40-60 ℃ of temperature, pulverizing obtains B group mixture;
3, finished product preparation
Be that the A group mixture of 65-70% is packed with the ratio thorough mixing final vacuum of the B group mixture of 30-35% and made finished product according to weight ratio.
Embodiment two
1, the preparation of A group mixture
1.1, with 8 degree Beaume worts 121 ℃ the sterilization 20 minutes, be cooled to 28 ℃, ratio by weight 10% inserts first order seed, constant temperature culture is 18 hours under 28 ℃ of temperature, 10% ratio inserts secondary seed by weight again, enlarged culturing obtained secondary seed solution in 24 hours under 28 ℃ of temperature, and described seed is respectively Candida utilis, geotrichum candidum seed;
1.2, according to weight ratio is wheat bran: dregs of beans: the mixed of rice husk=90: 8: 2 evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.1% potassium primary phosphate in the water, 0.05% sal epsom, its per-cent is the mass/volume ratio, under 100 ℃ temperature, steam 35 minutes, be cooled to 28 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of 20% Candida utilis and 20% adds microbial inoculum, at 28 ℃, suitably cultivated 35 hours under the airy condition, under 50 ℃ of temperature, be dried to water content below 15%, pulverizing obtains A group mixture;
2, the preparation of B group mixture:
2.1 according to glucose 1%, hydrolysis bean cake powder 1%, 5mgVB
2The mixed of/L, 0.5% sodium-chlor and excess water is made liquid seed culture medium, per-cent wherein is weight percentage, liquid seed culture medium was sterilized 20 minutes under 121 ℃ of temperature, be cooled to 35 ℃, according to weight ratio is 10% ratio inoculation first order seed, 35 ℃ of constant temperature culture 20 hours, according to weight ratio is that 10% ratio inserts secondary seed, 35 ℃ of enlarged culturing 36 hours, obtain secondary seed solution, described seed is respectively Corynebacterium glutamicum, digestion Bacterium lacticum, subtilis;
2.2 according to weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=20: 60: 18: 2 mixed evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, be added with 0.5% sodium-chlor in the water, its per-cent is the mass/volume ratio, under 100 ℃ of temperature, steam 25-35 minute, be cooled to 35 ℃, be Corynebacterium glutamicum by weight, each ratio of 15% of digestion Bacterium lacticum and subtilis liquid seeds liquid adds microbial inoculum, under 35 ℃ of suitable airy conditions, cultivated 48 hours, be dried to water content below 15% under 50 ℃ of temperature, pulverizing obtains B group mixture;
3, finished product preparation
To be 65% A group mixture with the ratio thorough mixing final vacuum of 35% B group mixture pack makes finished product according to weight ratio.
Claims (4)
1, a kind of livestock and poultry microecological microbial agent is characterized in that: it is that weight ratio is A group mixture and the 30-35%B group mixture composition of 65-70%; Wherein A group mixture is that weight ratio is a wheat bran: dregs of beans: rice husk=(90-100): (8-10): solid mixture (1-3), add the water that accounts for solid mixture weight 15-20%, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 45-50 ℃ of temperature, be dried to the mixture that water content 15% below, pulverizing obtain; B group mixture is that weight ratio is the high temperature dregs of beans: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): solid mixture (1-2), add the water that accounts for solid mixture weight 15-20%, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 45-50 ℃ of temperature, the mixture that pulverizing obtains.
2, a kind of preparation method of livestock and poultry microecological microbial agent is characterized in that:
(1), the preparation of A group mixture
(1.1), 8 degree Beaume worts were sterilized 20 minutes at 121 ℃, be cooled to 28 ℃, ratio by weight 10% inserts first order seed, constant temperature culture is 18 hours under 28 ℃ of temperature, 10% ratio inserts secondary seed by weight again, enlarged culturing obtained secondary seed solution in 24 hours under 28 ℃ of temperature, and described seed is respectively Candida utilis, geotrichum candidum seed;
(1.2), according to weight ratio is wheat bran: dregs of beans: rice husk=(90-100): (8-10): mixed (1-3) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, under 95-105 ℃ temperature, steam 25-35 minute, be cooled to 28-30 ℃, the ratio that according to weight ratio is the geotrichum candidum secondary seed solution of the Candida utilis of 20-25% and 20-25% adds microbial inoculum, at 28-30 ℃, suitably cultivated 30-35 hour under the airy condition, under 40-60 ℃ of temperature, be dried to water content below 15%, pulverizing obtains A group mixture;
(2), the preparation of B group mixture:
(2.1) according to glucose 1%, hydrolysis bean cake powder 1%, 5mgVB
2The mixed of/L, 0.5% sodium-chlor and excess water is made liquid seed culture medium, per-cent wherein is weight percentage, liquid seed culture medium was sterilized 20 minutes under 121 ℃ of temperature, be cooled to 35 ℃, according to weight ratio is 10% ratio inoculation first order seed, 35 ℃ of constant temperature culture 20 hours, according to weight ratio is that 10% ratio inserts secondary seed, 35 ℃ of enlarged culturing 36 hours, obtain secondary seed solution, described seed is respectively Corynebacterium glutamicum, digestion Bacterium lacticum, subtilis;
(2.2) be the high temperature dregs of beans according to weight ratio: wheat bran: Semen Maydis powder: rice husk=(15-20): (50-60): (18-20): mixed (1-2) evenly obtains solid mixture, in solid mixture, add the water that accounts for solid mixture weight 15-20%, under 95-105 ℃ of temperature, steam 25-35 minute, be cooled to 30-35 ℃, be Corynebacterium glutamicum by weight, the ratio of digestion Bacterium lacticum and each 15-20% of subtilis liquid seeds liquid adds microbial inoculum, under 30-35 ℃ of suitable airy condition, cultivated 45-50 hour, be dried to water content below 15% under the 40-60 ℃ of temperature, pulverizing obtains B group mixture;
(3), finished product preparation
Be that the A group mixture of 65-70% is packed with the ratio thorough mixing final vacuum of the B group mixture of 30-35% and made finished product according to weight ratio.
3, the preparation method of a kind of livestock and poultry microecological microbial agent according to claim 2, it is characterized in that: in the water that adds solid mixture to, add 0.1% potassium primary phosphate that accounts for water in the preparation process of A group mixture, 0.05% sal epsom, its per-cent are the mass/volume ratio.
4, according to the preparation method of claim 2 or 3 described a kind of livestock and poultry microecological microbial agents, it is characterized in that: add 0.5% sodium-chlor that accounts for water in the preparation process of B group mixture in the water that adds solid mixture to, its per-cent is the mass/volume ratio.
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| CN1222219C (en) * | 2003-05-26 | 2005-10-12 | 哈尔滨中冠生物工程有限公司 | Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation |
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| CN102757277A (en) * | 2012-07-31 | 2012-10-31 | 长春华御实业集团有限公司 | Paddy biofertilizer, preparation method thereof and bacillus subtilis |
| CN104605181A (en) * | 2015-01-06 | 2015-05-13 | 铁岭新东方饲料有限公司 | Environment-friendly compound feed for pig and preparation method of environment-friendly compound feed |
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| CN104839454B (en) * | 2015-05-28 | 2018-02-16 | 河南双成生物科技有限公司 | A kind of high protein feed production method rich in valine |
| CN105076708A (en) * | 2015-09-22 | 2015-11-25 | 河南科技大学 | Poultry yeast culture and preparation method thereof |
| CN108911879A (en) * | 2018-08-23 | 2018-11-30 | 北京市植物保护站 | A kind of bioactive functions reduction disinfection particle and sterilization method |
| CN114921399A (en) * | 2022-06-30 | 2022-08-19 | 哈尔滨市灵扩科技发展有限公司 | Preparation method of efficient culture livestock and poultry intestinal probiotics |
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